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Semen Analysis Manner of Collection

Semen analysis is used to assess fertility and involves examining a semen sample's volume, viscosity, pH, sperm concentration, motility, and morphology. It is important that the sample be collected properly via masturbation in a sterile container and kept at body temperature during transport to the lab for analysis within an hour. Parameters like appearance, volume, viscosity, and pH are checked initially, followed by examining sperm concentration, motility, and percentage of normal forms under a microscope to identify any abnormalities.

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384 views2 pages

Semen Analysis Manner of Collection

Semen analysis is used to assess fertility and involves examining a semen sample's volume, viscosity, pH, sperm concentration, motility, and morphology. It is important that the sample be collected properly via masturbation in a sterile container and kept at body temperature during transport to the lab for analysis within an hour. Parameters like appearance, volume, viscosity, and pH are checked initially, followed by examining sperm concentration, motility, and percentage of normal forms under a microscope to identify any abnormalities.

Uploaded by

lucky mark navarro
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Semen Analysis o Specimen awaiting analysis should be

kept at 37C
Seminal fluid or semen: Complex body fluid
used to transport sperm or spermatozoa Manner of Collection
o Masturbation
Uses of Semen Analysis o Non lubricating rubber or polyurethane
o Use to asses in advance of andrology condoms
o Concerns over fertility
o Post vasectomy semen analysis All specimen are possible reservoirs of infection
o Forensic analyses Specimens are discarded as biohazardous
waste
Semen Composition Sterile materials and techniques must be used:
o Spermatozoa: 5% o Semen culture
o Seminal Fluid: 60 – 70% o Bioassay
o Prostate Fluid: 20 – 30% o Intra uterine insemination
o Bulbourethral Glands: 5% o In vitro fertilization

Specimen Collection and Handling Parameters


o Most sperm contained first portion of o Appearance
ejaculate o Volume
o First portion MISSING = Sperm count o Viscosity
decrease, pH falsely decrease, no o pH
liquefaction o Sperm concentration and count
o Last portion MISSING = Semen volume o Motility and morphology
decrease, sperm count increase, pH
falsely decrease, no coagulation Appearance
o Specimen collected following sexual Normal: Gray white color
abstinence (atleast 2 days, not >7 days) Low sperm conc: Almost clear
o Prolong abstinence = higher volume, WBC, infection in urinary tract: White turbidity
decrease motility (spx culturing prior to continuing SA)
o Fertility testing WHO INCREASE RBC: Red semen
2 – 3 samples should be collected not less than INCREASE urine contamination, prolong
7 days and not more than 3 weeks apart abstinence and medication: Yellow semen
2 abnormal samples are considered significant
Liquefaction
Collection 30 – 60 minutes normal liquefaction time
o Sterile glass or plastic container Failure to liquefy may be caused by a deficiency
o Private room (spx must be kept at room in prostatic enzymes
temp and deliver within 1 hr) If after 2 hours, the specimen has not liquefy,
o Basic Information: Name, Birth date, you can use equal volume
Period of sexual abstinence, Dulbecco’s phosphate buffered saline
Completeness of sample, Difficulties Alpha chymotrypsin or Bromelain
with collection, Specimen receipt Jellylike granules are of no clinical significance
Volume Sperm Morphology
Normal: 2 – 5 mL >30% of normal forms: ROUTINE CRITERIA
INCREASE VOLUME: Prolong abstinence >14% of normal forms: STRICT CRITERIA
DECREASE VOLUME: Infertility, improper
functioning of semen producing organs, Head:
incomplete specimen collection Acrosome: Contain digestive enzymes
Nucleus: Contain23 chromosome
Viscosity
Refers to the consistency of the fluid & may be Middle piece
related to specimen liquefaction Collar: Containing many mitochondria
Incompletely liquefied spx are clumped and
highly viscous Tail:
Normal semen should be easily drawn into a Flagellum: Causes sperm to swim
pipette
Droplets that form >2cm is considered highly Additional testing
viscous
Abnormal Possible Test
pH Result Abnormalit
Should be measured within 1 hour y
Normal pH: 7.2 – 8.0 Decreased Vitality Eosin nigrosine
INCREASE: Infection of reproductive tract motility with stain
DECREASE: Prostatic fluid, ejaculatory duct normal count
obstruction, poorly developed seminal vesicles Decreased Lack of Fructose level
pH pad of urinalysis reagent trip can be used count seminal
vesicle
Sperm Concentration and Sperm Count support
Normal concentration: >20 million/mL medium
Borderline: 10 – 20 million/mL Decreased Male Mixed
Total sperm count = sperm concentration x motility with antisperm agglutination
specimen volume >40 million/ejaculate clumping antibodies reaction &
Dilution of the spx is essential because it immunobead
immobilized the sperm prior to counting test

Sperm Motility Sperm


4.0 A Rapid, straight line motility agglutination
3.0 B Slower speed, some lateral with male serum
movement Normal Female Sperm
2.0 B Slow forward progression, analysis with antisperm agglutination
noticeable lateral movement continued antibodies with female
1.0 D No forward progression motility serum
0 D No movement

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