Supelco Columns for USP Methods

The official pharmaceutical analysis monographs in the United States Pharmacopeia (USP) detail the methods used by
pharmaceutical manufacturers for quality control of bulk drug substances and dosage form preparations. Each method
specifies a particular high pressure liquid chromatography (HPLC) or gas chromatography (GC) column or column type and
the conditions under which the analysis is performed. This poster lists the USP Codes for the phases and supports used in
these methods, descriptions of the columns, and information about the Supelco products that conform to these descriptions.

HPLC Packings USP Code

G14
Description
Polyethylene glycol (av. mol. wt. of 950 to 1,050)
Recommended Phase
• Carbowax® 1000
USP Code Description Recommended Packing
G15 Polyethylene glycol (av. mol. wt. of 3000 to 3,700) • Carbowax 4000
G16 Polyethylene glycol compound (av. mol. wt. about 15,000). • Carbowax 20M
L1 Octadecyl silane chemically bonded to porous or non- • Titan™ C18 • Discovery HS F5 A high molecular weight compound of polyethylene glycol • Omegawax® (capillary)
porous silica or ceramic micro-particles, 1.5 to 10 μm in • Ascentis® C18 • Discovery BIO Wide Pore C18 with a diepoxide linker. • SUPELCOWAX® 10 (capillary)
diameter, or a monolithic rod. • Ascentis Express C18 • SUPELCOSIL™ LC-18 G17 75% Phenyl-25% methylpolysiloxane • OV-25
• BIOshell ™ Peptide C18 • SUPELCOSIL LC-18-DB G18 Polyalkylene glycol • UCON™ LB-550-X • PAG (capillary)
• Discovery® C18 • SUPELCOSIL LC-318 • UCON LB-1800-X
L3 Porous silica particles, 1.5 to 10 μm in diameter, or a • Ascentis Express HILIC • SUPELCOSIL LC-Si G19 25% Phenyl-25% cyanopropyl-50% methylsilicone • OV-225
monolithic silica rod. • Ascentis Si • SUPELCOSIL LC-3Si • SPB-225 (capillary)
L7 Octylsilane chemically bonded to totally or superficially • Ascentis C8 • SUPELCOSIL LC-8 G20 Polyethylene glycol (av. mol. wt. of 380 to 420) • Carbowax 400
porous silica particles, 1.5 to 10 μm in diameter, or a • Ascentis Express C8 • SUPELCOSIL LC-8-DB
monolithic silica rod. • Discovery C8 • SUPELCOSIL LC-308 G21 Neopentyl glycol succinate • Neopentyl glycol succinate
• Discovery BIO Wide Pore C8 G22 Bis(2-ethylhexyl) phthalate • Bis(2-ethylhexyl)phthalate
L8 An essentially monomolecular layer of amino-propylsilane • SUPELCOSIL LC-NH2 G23 Polyethylene glycol adipate • Ethylene glycol adipate (EGA)
chemically bonded to totally porous silica gel support, 1.5 • SUPELCOSIL LC-NH2-NP G24 Diisodecyl phthalate • Diisodecyl phthalate
to 10 μm in diameter. G25 Polyethylene glycol compound TPA. A high molecular • Carbowax 20M-terephthalic • Free Fatty Acid Phase (FFAP)
L9 Irregular or spherical, totally porous silica gel having a • SUPELCOSIL LC-SCX weight compound of a polyethylene glycol and a acid • SPB-1000 (capillary)
chemically bonded, strongly acidic cation-exchange diepoxide that is esterified with terephthalic acid. • Carbowax 20M-TPA • Nukol™ (capillary)
coating, 3 to 10 μm in diameter. • SP-1000
L10 Nitrile groups chemically bonded to porous silica particles, • Ascentis ES Cyano • SUPELCOSIL LC-CN G27 5% Phenyl-95% methylpolysiloxane • SE-52 • Equity-5 (capillary)
1.5 to 10 μm in diameter. • BIOshell Peptide CN • SUPELCOSIL LC-PCN • SLB®-5ms (capillary) • SPB-5 (capillary)
• Discovery Cyano G28 25% Phenyl-75% methylpolysiloxane • DC-550
L11 Phenyl groups chemically bonded to porous silica particles, • Ascentis Phenyl • SUPELCOSIL LC-DP G29 3,3’-Thiodipropionitrile • ßß’-Thiodipropionitrile (TDPN)
1.5 to 10 μm in diameter. • Ascentis Express Phenyl-Hexyl • SUPELCOSIL LC-3DP G30 Tetraethylene glycol dimethyl ether • Tetraethylene glycol dimethyl ether
L13 Trimethylsilane chemically bonded to porous silica • SUPELCOSIL LC-1 G31 Nonylphenoxypoly(ethyleneoxy)ethanol (av. ethyleneoxy • IGEPAL® CO-880 (Nonoxynol)
particles, 3 to 10 μm in diameter. chain length is 30); Nonoxynol 30
L14 Silica gel having a chemically bonded, strongly basic • SUPELCOSIL SAX1 G32 20% Phenylmethyl-80% dimethylpolysiloxane • OV-7
quaternary ammonium anion-exchange coating, 5 to 10 • SPB-20 (capillary)
μm in diameter. G33 20% Carborane-80% methylsilicone • Dexsil® 300
L17 Strong cation-exchange resin consisting of sulfonated • SUPELCOGEL™ C-610H • Proteomix® WCX G34 Diethylene glycol succinate polyester stabilized with • DEGS-PS
cross-linked styrene-divinylbenzene copolymer in the • SUPELCOGEL H phosphoric acid
hydrogen form, 6 to 12 μm in diameter.
G35 A high molecular weight compound of polyethylene glycol • Carbowax 20M-terephthalic • Free Fatty Acid Phase (FFAP)
L19 Strong cation-exchange resin consisting of sulfonated • SUPELCOGEL Ca and a diepoxide that is esterified with nitroterephthalic acid acid • SPB-1000 (capillary)
cross-linked styrene-divinylbenzene copolymer in the • Proteomix SCX • Carbowax 20M-TPA • Nukol (capillary)
calcium form, about 9 μm in diameter. • SP-1000
L20 Dihydroxypropane groups chemically bonded to porous • Kromasil® Diol • SW mAb series G36 1% Vinyl-5% phenylmethylpolysiloxane • SE-54 • Equity-5 (capillary)
silica or hybrid particles, 1.5 to 10 μm in diameter. • SUPELCOSIL LC-Diol • SWxl • SLB-5ms (capillary) • SPB-5 (capillary)
• SuperSW • TSKgel QC-PAK GFC G38 Phase G1 containing a small percentage of a tailing inhibitor • SP-2100 + 0.1% Carbowax 1500
• SW • SP-2100 + 0.2% Carbowax 1500
L21 A rigid, spherical styrene-divinylbenzene copolymer, 3 to • Hhr • SuperHZ G40 Ethylene glycol adipate • Ethylene glycol adipate (EGA)
10 μm in diameter. • PRP-1 • SuperMultiporeHZ series G41 Phenylmethyldimethylsiloxane (10% phenyl-substituted) • OV-3
• SuperH • TSKgel Hxl
L22 A cation-exchange resin made of porous polystyrene gel • SUPELCOGEL C-610H • PRP-X200 G42 35% Phenyl-65% dimethylpolysiloxane (percentages refer • OV-11
with sulfonic acid groups, about 10 μm in size. • SUPELCOGEL H • PRP-X300 to molar substitution) • SPB-35 (capillary)
L23 An anion-exchange resin made of porous polymethacrylate • Discovery BIO PolyMA-WAX G43 6% cyanopropylphenyl-94% dimethylpolysiloxane • OV-1301 • SPB-624 (capillary)
or polyacrylate gel with quaternary ammonium groups,
(percentages refer to molar substitution) • OVI-G43 (capillary)
7 to 12 μm in size. G44 2% low molecular weight petrolatum hydrocarbon grease • Apiezon® L + 1% KOH
and 1% solution of potassium hydroxide
L26 Butyl silane chemically bonded to totally porous silica • BIOshell Protein C4
particles, 1.5 to 10 μm in diameter. • SUPELCOSIL LC-304 G45 Divinylbenzene-ethylene glycol-dimethylacrylate • HayeSep® A • Porapak™ N
L27 Porous silica particles, 30 to 50 μm in diameter. • Discovery DSC-Si • Pelliguard™ LC-Si • HayeSep N
• Supelclean™ LC-Si G46 14% Cyanopropylphenyl-86% methylpolysiloxane • OV-1701
L32 A chiral ligand-exchange resin packing-L-proline copper • Astec® CLC-D • Equity-1701 (capillary)
complex covalently bonded to irregularly shaped silica • Astec CLC-L G47 Polyethylene glycol (av. mol. wt. of about 8,000) • Carbowax 8000
particles, 5 to 10 μm in diameter. G48 Highly polar, partially cross-linked cyanopolysiloxane • SP-2380
L34 Strong cation-exchange resin consisting of sulfonated • SUPELCOGEL Pb • SP-2380 (capillary)
cross-linked styrene-divinylbenzene copolymer in the lead
form, about 7 to 9 μm in diameter.
L38 A methacrylate-based size exclusion packing for water-
soluble samples.
• TSKgel PWxl
• PW
• Alpha
• SuperAW series GC Supports
• PWxl-CP • SuperMultiporePW USP Code Description Recommended Support
L40 Cellulose tris-3,5-dimethylphenylcarbamate coated porous • Astec Cellulose DMP S1A Siliceous earth for gas chromatography has been flux- • SUPELCOPORT®
silica particles, 5 to 20 μm in diameter. • Kromasil® CelluCoat® calcined by mixing diatomite with Na2CO3 flux and • Chromosorb® W AW
L41 Immobilized α1-acid glycoprotein on spherical silica • Chiral-AGP calcining above 900 °C. The siliceous earth is acid-washed, • Chromosorb W HP
particles, 5 μm in diameter. then water-washed until neutral, but not base-washed. The
L43 Pentafluorophenyl groups chemically bonded to silica • Ascentis Express F5 siliceous earth may be silanized by treating with an agent
particles by a propyl spacer, 5 to 10 μm in diameter. • Discovery HS F5 such as dimethyldichlorosilane to mask surface silanol
L45 Beta cyclodextrin bonded to porous silica particles, 5 to • Astec CYCLOBOND® I 2000 Series groups. Note: Unless otherwise specified in the individual
10 μm in diameter. monograph, silanized support is intended.
L49 A reversed-phase packing made by coating a thin layer of • Discovery Zr-PBD S1AB The siliceous earth as described above is both acid- and • SUPELCOPORT BW
polybutadiene onto spherical porous zirconia particles, 3 to base-washed. Note: Unless otherwise specified in the individual
10 μm in diameter. monograph, silanized support is intended.
L52 A strong cation exchange resin made of porous silica with • SUPELCOSIL LC-SCX S1C A support prepared from crushed firebrick and calcined or • Chromosorb P AW
sulfopropyl groups, 5 to 10 μm in diameter. burned with a clay binder above 900 °C with subsequent • Chromosorb P AW-DMCS
L59 Packing for the size-exclusion separations of proteins • TSKgel: G2000SW, G3000SW, G4000SW, G2000SWxl, acid-wash. It may be silanized.
(separation by molecular weight) over the range of 5 to G3000SWxl, G4000SWxl, SuperSW2000, SuperSW3000, S1D A support prepared from crushed firebrick and calcined or • Chromosorb P NAW
7,000 kDa. It is spherical (1.5 to 10 μm), silica or hybrid SuperSW mAb HR and UltraSW Aggregate burned with a clay binder above 900 °C, not acid washed.
packing with a hydrophilic coating. • Sepax: SRT SEC-100, SRT SEC-150, SRT SEC-300, SRT SEC-500 It may be silanized.
L60 Spherical, porous silica gel, 10 μm or less in diameter, the • Ascentis RP-Amide • SUPELCOSIL ABZ+PLUS S1NS The siliceous earth is untreated. • Chromosorb W NAW
surface of which has been covalently modified with alkyl • Ascentis Express RP-Amide • SUPELCOSIL LC-ABZ S2 Styrene-divinylbenzene copolymer having a nominal • Chromosorb 101
amide groups and endcapped. • Discovery RP-AmideC16 surface area of less than 50 m2 per g and an average pore
L63 Glycopeptide teicoplanin linked through multiple covalent • Astec CHIROBIOTIC® T • Astec CHIROBIOTIC TAG diameter of 0.3 to 0.4 μm.
bonds to a 100 Å units spherical silica. • Astec CHIROBIOTIC T2 S3 Copolymer of ethylvinylbenzene and divinylbenzene • HayeSep Q
L67 Porous vinyl alcohol copolymer with a C18 alkyl group • apHera™ C18 having a nominal surface area of 500 to 600 m2 per g and • Porapak Q
attached to the hydroxyl group of the polymer, 2 to 10 μm an average pore diameter of 0.0075 μm. • Super Q
in diameter. S4 Styrene-divinylbenzene copolymer with aromatic –O and • HayeSep R
L68 Spherical, porous silica, 10 μm or less in diameter, the • Suplex™ pKb-100 –N groups, having a nominal surface area of 400 to 600 m2 • Porapak R
surface of which has been covalently modified with alkyl per g and an average pore diameter of 0.0076 μm.
amide groups and not endcapped. S5 40- to 60-mesh, high molecular weight tetrafluorethylene • Chromosorb T
L88 Glycopeptide vancomycin linked through multiple • Astec CHIROBIOTIC V polymer.
covalent bonds to a 100 Å spherical silica. • Astec CHIROBIOTIC V2 S6 Styrene-divinylbenzene copolymer having a nominal • HayeSep P
surface area of 250 to 350 m2 per g and an average pore • Porapak P
diameter of 0.0091 μm. • Chromosorb 102
GC Phases S7 Graphitized carbon having a nominal surface area of
12 m2 per g.
• Carbopack™ C
USP Code Description Recommended Phase S8 Copolymer of 4-vinyl-pyridine and styrene-divinylbenzene. • HayeSep S
G1 Dimethylpolysiloxane oil • SP®-2100 • Equity®-1 (capillary) • Porapak S
• OV®-101 • SPB®-1 (capillary) S9 A porous polymer based on 2,6-diphenyl-p-phenylene oxide. • Tenax® TA
• SE-30 S10 A highly polar cross-linked copolymer of acrylonitrile and • HayeSep C
divinylbenzene.
G2 Dimethylpolysiloxane gum • SP-2100 • Equity-1 (capillary)
• OV-1 • SPB-1 (capillary) S11 Graphitized carbon having a nominal surface area of • 3% SP-1500 on 80/120 Carbopack B
• SE-30 100 m2 per g modified with small amounts of petrolatum
and polyethylene glycol compound.
G3 50% Phenyl-50% methylpolysiloxane • SP-2250 • SPB-50 (capillary)
• OV-17 S12 Graphitized carbon having a nominal surface area of • Carbopack B
100 m2 per g.
G4 Diethylene glycol succinate polyester • Diethylene glycol succinate (DEGS)
Supelco’s Molecular sieve 5A GC material meets USP/NF criteria for analysis of nitrogen purity: “…a molecular sieve prepared from a synthetic alkali-metal aluminosilicate
G5 3-Cyanopropylpolysiloxane • SP-2340 • SP-2340 (capillary) capable of absorbing molecules having diameters of up to 0.5 nm, which permit complete separation of oxygen from nitrogen.”
• Silar 10 CP • SP-2560 (capillary)
G6 Trifluoropropylmethylpolysiloxane • SP-2401 Contact our Technical Service Department for expert answers to your questions.
• OV-210 Phone: 800-325-5832; Fax: 314-286-7828; email: [email protected]
G7 50% 3-Cyanopropyl-50% phenylmethylsilicone • SP-2300 • SPB-225 (capillary) Footnotes:  Indicates availability of material(s) matching the description. Supelco is not necessarily the manufacturer of the material.
• Silar 5 CP  Unless otherwise specified, mesh sizes of 80 to 100 or, alternatively, 100 to 120 are intended.
G8 80% Bis(3-cyanopropyl)-20% 3-cyanopropylphenyl- • SP-2330 Reference: United States Pharmacopeia 37, National Formulary 32, (November 1, 2013). Request from United States
polysiloxane (percentages refer to molar substitution) • SP-2330 (capillary) Pharmacopeial Convention, Inc., 12601 Twinbrook Parkway, Rockville, MD USA 20852 (tel. 800-227-8772).
G9 Methylvinylpolysiloxane • OV-1 • Equity-1 (capillary)
• UCW 98 • SPB-1 (capillary) For more information, visit
G11 Bis(2-ethylhexyl)sebacate polyester • Di(2-ethylhexyl)sebacate sigma-aldrich.com/analytical
G12 Phenyldiethanolamine succinate polyester • Phenyldiethanolamine succinate
G13 Sorbitol • Sorbitol

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apHera, BIOShell, Carbopack, Nukol, Pelliguard, Solutions within, Supelclean, SUPELCOGEL, SUPELCOSIL, Suplex and Titan are trademarks of Sigma-Aldrich Co. LLC. Apiezon is a registered trademark of M & I Materials Ltd. Carbowax is a registered trademark of Union Carbide. Ucon is a trademark of Union Carbide. CelluCoat and Kromasil are FWK
registered trademarks of EKA Chemicals AB. Chromosorb is a registered trademark of Imerys Minerals California, Inc. Dexsil is a registered trademark of Dexsil Corp. HayeSep is a registered trademark of Hayes Separation Inc. IGEPAL is a registered trademark of Rhodia Operations. OV is a registered trademark of Ohio Valley Specialty Chemical Co. 11237 / T403109E
PoraPak is a trademark of Waters. Proteomix is a registered trademark of Sepax Technologies. Tenax is a registered trademark of Buchem B.V. Supelco brand products are sold by affiliated Sigma-Aldrich distributors. 1114