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Dialysis Techniques in Biochemistry Lab

This document summarizes a laboratory experiment on dialysis. The objectives are to determine the properties of water that make it suitable for biological systems and to demonstrate dialysis. Dialysis works by diffusion of molecules through a semipermeable membrane. Tests are described to show that water is a universal solvent and good medium for biochemical reactions by dissolving various substances. The experiment involves soaking a dialysis bag containing starch and NaCl solution, then testing the external water to see if chloride ions diffuse out, confirming dialysis occurred.

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Reñella Sapasap
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184 views2 pages

Dialysis Techniques in Biochemistry Lab

This document summarizes a laboratory experiment on dialysis. The objectives are to determine the properties of water that make it suitable for biological systems and to demonstrate dialysis. Dialysis works by diffusion of molecules through a semipermeable membrane. Tests are described to show that water is a universal solvent and good medium for biochemical reactions by dissolving various substances. The experiment involves soaking a dialysis bag containing starch and NaCl solution, then testing the external water to see if chloride ions diffuse out, confirming dialysis occurred.

Uploaded by

Reñella Sapasap
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Our Lady of Fatima University – Valenzuela Campus

College of Medical Laboratory Science

Biochemistry for Medical Laboratory Science – Laboratory


Reñella Sapasap | September 18, 2021 | 1st Semester – Prelim

❖ Dialysis is a common laboratory technique widely ❖ Water is important to all living systems. It serves
used for removing contaminants from solutions. as natural solvent for mineral ions and other
❖ Dialysis techniques is commonly used to remove substances. It is also the dispersion medium for
small unwanted molecules such as colloidal cells like protoplasm. It serves as the
➢ Salts medium for most biochemical reactions and is the
➢ Reducing agents most abundant component of the cells. Except for
➢ Preservatives bone tissues and enamel, water constitutes about
❖ Dialysis works by selective and passive diffusion 70 percent of the human body.
through a semipermeable membrane. ❖ The objective of this experiment is to determine
❖ Dialysis can also be used for buffer exchange. the properties of water that make it a suitable
➢ The buffer used for dialysis is called dialysate. medium for sustaining life in biological system.
❖ The materials needed for this experiment are
the following

1% NaCl in Starch
❖ Dialysis works by the principle of diffusion.
Solution Gelatin
❖ Diffusion is the migration of molecules randomly 10% Sucrose Solution Lard
from areas of higher concentration to lower 250 ml Beaker (2) 0.1M AgNO 3
concentration until equilibrium is reached. CCl4 NaCl
❖ In Dialysis, the migration of molecules occurs Cellophane NaHCO3 powder
through a semipermeable membrane, which allow Citric Acid powder String or Rubber Band
only small molecules to pass through restricting CuSO4 Sugar
the movement of large molecules like proteins. Deionized Water Test Tube Rack
Dialysis Bag Test Tubes (6)
Ethanol Thistle Tube

❖ Water: A Universal Solvent


➢ Put about 0.5 grams of the following
substances into six separate test tubes: NaCl,
sugar, gelatin, CuSO 4, lard, and ethanol. Add
1 ml of water to each test tube and shake
vigorously to dissolve the substances. For
substances that did not dissolve, add another 1
ml of water, and shake again. For the solids
that still did not dissolve, add another 1 ml
water and shake.
➢ On a six separate test tube, Repeat the
solubility test using CCl 4, instead of water.
➢ Describe solubility in both solvents as soluble,
slightly soluble, and insoluble. Record
observations in the table.
❖ Water: A Good Medium for Biochemical
Reactions
REÑELLA SAPASAP 1
|CHEM123 LAB| Dialysis |Prof. Shiela Mai S. Rocabo, RMT, MSMT|

➢ Mix 0.1 gram of dry, powdered citric acid, and


sodium bicarbonate (NaHCO3) in a dry test
tube. Observe if a chemical reaction occurs.
➢ Add about 10 ml of water to the mixture and
note what happens.
❖ Properties of Water Solutions
➢ Dialysis
▪ Obtain a dialysis bag about 20 to 25 cm
long and soak in clean water for about 10
minutes.
▪ Fill with 30 ml of 1% starch-NaCl mixture,
tie the bag and rinse thoroughly with
water. Put the bag in a beaker containing
deionized water.
▪ Adjust the setup such that the levels of
fluids inside and outside the bag are the
same.
▪ After 1 hour, test 1 ml of dialysate (water
in the beaker) with a few drops of 0.1M
AgNO3. Formation of a white precipitate
of AgCl confirms the presence of chloride
ions in dialysate.

REÑELLA SAPASAP 2

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