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Fish Oil

The document discusses a study on extracting fish oil from milkfish satay byproduct using dry rendering extraction. The study tested extraction at 3 temperatures (40°C, 50°C, 60°C) and analyzed yield, free fatty acids, peroxide value, p-anisidine value and total oxidation of the extracted oils. The best extraction conditions were 50°C, yielding an oil with 6.88% yield, 4.89% free fatty acids, 29.35 mEq/kg peroxide value, 4.61 mEq/kg p-anisidine value and 63.53 mEq/kg total oxidation.
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0% found this document useful (0 votes)
78 views7 pages

Fish Oil

The document discusses a study on extracting fish oil from milkfish satay byproduct using dry rendering extraction. The study tested extraction at 3 temperatures (40°C, 50°C, 60°C) and analyzed yield, free fatty acids, peroxide value, p-anisidine value and total oxidation of the extracted oils. The best extraction conditions were 50°C, yielding an oil with 6.88% yield, 4.89% free fatty acids, 29.35 mEq/kg peroxide value, 4.61 mEq/kg p-anisidine value and 63.53 mEq/kg total oxidation.
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Fitriani, Food

et. al.ScienTech Journal Vol. 3 (2) 2021 ISSN : 2685-4279 DOI:10.33512/fsj.v3i2.12906

PRELIMINARY STUDY OF FISH OIL FROM MILKFISH SATAY BY PRODUCT


USING DRY RENDERING EXTRACTION
Hanifah Fitriani, Aris Munandar, Dini Surilayani, Sakinah Haryati, Ginanjar Pratama,
Bhatara Ayi Meata, Afifah Nurazizatul Hasanah, and Rifki Prayoga Aditia*

Fisheries Science Study Program, Sultan Ageng Tirtayasa University, Indonesia


*E-mail: [email protected]

ABSTRACT

Milkfish satay processing has been left viscera waste that may causes environmental
pollution. The viscera waste has contained omega-3 which can be extracted as fish oil.
Dry rendering is a method of fish oil extracting using temperature without water
addition. The temperature of extraction greatly affects to quality of fish oil. The purpose
of this study was to determine the optimal temperature of extraction and characterized
fish oil quality extracted from milkfish viscera. This study used dry rendering extraction
methode with three different temperatures (40ºC, 50ºC, and 60ºC) and tested the yield,
free fatty acids, peroxide value, p-anisidine and total oxidation, for the best fish oil will
be tested for its fatty acid profile. The best treatment for extracting fish oil from milkfish
viscera used extraction temperature of 50ºC with yield (6.88%), free fatty acid (4.89%)
peroxide value (29.35 mEq/kg), anisidine value (4.61 mEq/kg), and total oxidation
(63.53 mEq/kg). The fatty acid profile of fish oil was dominated by palmitic acid
(31.17%) and also contains omega-3 such as linoleic acid, docosahexaenoate acid
(DHA), and eicosapentaenoate acid (EPA).

Keywords: dry rendering extraction, milkfish visceral, temperature

INTRODUCTION 3 such as Eicosapentaenoic Acid (EPA) and


Serang City, Banten is one of the Docosahexaenoic Acid (DHA).
centers for milkfish product precessing with Fish oil is a fat component in fish body
milkfish raw material that needs 133.14 tissue and produced by extraction, one of the
tons/year (DKP 2017). One of the populer fish oil extraction methods is the dry
products is milkfish satay. The milkfish satay rendering method which uses temperature
processing produced viscera waste. treatment without the addition of water
According to DKP (2017) the amount of (Estiasih 2009). According to Putra (2018),
viscera waste that produced in milkfish satay temperature greatly affects the fish oil
processing in Serang were of 49.6 kg/day. extraction process and the use of high
Milkfish satay processing waste has not temperatures (>70ºC) can cause quality
used optimally, if not managed properly will degradation that affects the quality of fish oil
have a negative impact on the environment. that does not comply with the International
The most effective and efficient alternative to Fish Oil Standard (IFOS (2011). The purpose
solve this problem is optimizing the of this study was to determine the optimal
utilization of viscera waste to be fish oil. temperature and characterize the quality of
Suseno (2014) viscera of fish contain omega- fish oil extraction from milkfish viscera.

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Preliminary study of fish oil ..

MATERIALS AND METHODS Fish oil weight (g)


% Yield= × 100%
Materials Viscera weight (g)
Viscera of milkfish collected from the
processing industry of milkfish satay, located Determination of Peroxide Value (BSN,
at Serang, Banten. The chemical material 2018)
used in this study are ethanol 96%, n-heksan Peroxide value was calculated based on
(Merck), phenolphthalein indicator, aquades, the following equation:
NaOH (Merck) 0.1 N, chloroform (Merck), S × M × 1000
Peroxide value (mEq/kg) =
acetic acid glacial (Merck), anisidine reagent Sample weight (g)
(Aldrich chemistry), Bromine Cresol Green-
Methyl Red indicator, KOH (Merck) 0.1 N, S = Amount of sodium thiosulfate (mL)
KI, starch 1%. The tools used in this study are M = Sodium thiosulfate concentration (0,01
oven, sieve, blender, knife, measuring cup, N)
tray, alumunium foil and others.
Determination of Free Fatty Acid (AOAC,
Sample Preparation 2005)
The viscera of milkfish were taken Free fatty acid was calculated based on
from the milkfish satay processing industry. the following equation:
The viscera transported to the laboratory by A×N×M
% Free fatty acid =
being put into a cool box with the addition of 10G
ice (1:1 w/w). The sample washed with water
and crushed using blender, then weighed. A = Amount of KOH titration (mL)
N = Normality of KOH (0,1 N)
Extraction Methods G = Sample weight (g)
This extraction used dry rendering M = Dominant fatty acid molecular weight
extraction method according to Rozi et al.
(2016). Viscera milk fish was heated in an Determination of p-anisidine value
oven with a temperature variation of 40°C, (Watson, 1994)
50°C and 60°C for 8 hours and then pressed Determination of anisidine value was
for collected the fish oil. The extract of fish calculated based on the following equation:
oil was stored in the refrigerator for 5 minutes
25 x (1,2 A2 - A1)
at 15 °C, then the fish oil was centrifuged at Anisidine value (mEq/kg) =
weight of sample
4.000 rpm for 15 minutes. After that, the fish
oil was weighed and put into a dark glass
A1 = Absorbance of test solution 1
bottle that had been coated with aluminum
A2 = Absorbance of test solution 2
foil, then stored at low temperature. The
optimum extraction temperature was
Determination of Total Oxidation Value
determined from the results of the yield test,
(AOCS, 1998)
peroxide value (PV), free fatty acid (FFA),
The total oxidation value was obtained
anisidine value (p-AV) and total oxidation.
by adding twice the peroxide number plus the
The best temperature treatment would be
anisidine value. The total oxidation value is
characterized by the fatty acid profile.
calculated based on the following equation:
Total oxidation = 2PV + p-AV
Determination of Yield
PV = Peroxide value (mEq/kg)
The fish oil yield was calculated based
p-AV = Anisidine value (mEq/kg)
on the following equation:

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Fitriani, et. al.

Determination of Fatty Acid profile on the value of free fatty acids in the fish oil
(AOAC, 2005) produced (Table 2). The value of free fatty
Analysis of the fatty acid profile using acids of fish oil in this study does not meet
a gas chromatograph. This tool uses the the standard of free fatty acids in
principle of converting fatty acids into consumption fish oil set by IFOS (2011),
methyl esters which are more volatile. The which is <1.13%. Free fatty acids are
transformation of this research sample was produced due to hydrolysis of triglycerides so
carried out through methylation to obtain that fatty acids are released from bonds with
Fatty Acid Methyl Ester (FAME) which was glycerol and also caused by splitting and
then analyzed by gas chromatography. oxidation of fatty acid double bonds (Crexi et
al. 2010; Deepika et al. 2014). Increased
Data Analysis hydrolysis can increase the potential for
The experiment was run in triplicate damage oil so that the oil smells rancid
with a completely randomized design. Fish (Kamini et al. 2016).
oil data from dry rendering extraction (yield,
PV, FFA, p-anisidine, total oxidation value) Table 1. Yield of viscera fish oil
were subjected to analysis of variance Temperature (oC) Yield (%)
(ANOVA). The differences among the data 40 2,88 ± 0,35a
were determined using Duncan's multiple 50 6,88 ± 0,32b
range test (DMRT). 60 7,54 ± 0,81b
*Different superscript letters in the same column indicate a
significant difference (p<0,05)
RESULTS AND DISCUSSION
Yield
Table 2. Free fatty acid value of viscera fish oil
The results showed that the difference
Temperature (oC) Free Fatty Acid
in extraction temperature had a significant
Value (FFA) (%)
effect (p<0.05) on the percentage of fish oil
40 10,47 ± 0,36c
yield (Table 1). The higher of extraction
50 4,89 ± 0,36a
temperature would increase the yield of fish
60 5,86 ± 0,18b
oil produced. This is presumably because the *Different superscript letters in the same column indicate a
temperature in the heating process affects the significant difference (p<0,05)
protein denaturation of the raw material.
According to Nugroho et al. (2014) high Table 3. Peroxide value of vicera fish oil
temperature heating can damage protein and Temperature (oC) Peroxide Value
cell membranes, making it easier for
(mEq/kg)
deposited fat to come out more easily.
According to Triyono (2010) protein 40 25,58 ± 0,26a
denaturation will occur in the heating process 50 29,35 ± 0,76b
at a temperature of 50ºC-80ºC and every 60 46,50 ± 0,88c
10ºC increase in temperature the protein *Different superscript letters in the same column indicate a
significant difference (p<0,05)
denaturation rate can reach 600 times, so the
higher the temperature in the extraction
process the greater the yield of oil produced. Peroxide Value
The results showed that the extraction
Free Fatty Acid Value (FFA) temperature had a significant effect (p<0.05)
The results showed that the extraction on the the peroxide value of the fish oil
temperature had a significant effect (p<0.05) produced (Table 3). The higher of extraction
temperature will affect increasing peroxide

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Food Scientech Journal 3(2) 2021
Preliminary study of fish oil ..

value. The fish oil peroxide value from IFOS (2011) standard, which is 15 mEq/kg.
milkfish satay waste does not meet the The anisidine value is an indicator of
consumption fish oil standard set by IFOS secondary oxidation, so the higher the
(2011), which is 3.75 mEq/kg. peroxide value produced from the primary
According to Aidos et al. (2002) and oxidation process, the faster it decomposes
Suseno et al. (2015) temperature is one of the into secondary oxidation products. (Panagan
supporting factors that can accelerate the et al. 2012, Deepika et al. 2014).
oxidation process in fish oil. The value of
peroxide is very dependent on the extraction Total Oxidation Value
temperature, the higher the temperature used, The results showed that the extraction
the faster the oxidation process occurs. The temperature had a significant effect (p<0,05)
higher peroxide value indicates the level of on the total oxidation value of fish oil (Table
damage to fish oil. Peroxide levels are closely 5). The higher of extraction temperature
related to the quantity of hydroperoxides. causes increasing of total oxidation value.
Hydroperoxides can occur due to the The total oxidation value in this study did not
presence of double bonds (unsaturated fatty meet the consumption fish oil standard set by
acids) in the oil that binds oxygen from the IFOS (2011), which is 20 mEq/kg. According
surrounding air. Other factors that affect the to Kamini et al. (2016), the total oxidation
peroxide value are the type and freshness of value was used to estimate lipid oxidative
the raw materials used, the length of storage damage. The total oxidation value is
and the extraction method used. influenced by the primary oxidation value
(peroxide number) and secondary oxidation
Table 4. Anisidine value of viscera fish oil value (anisidine). The higher of peroxide
Temperature (°C) Anisidine Value value and anisidine value causes increasing
(mEq/kg) of total oxidation value.
40 10,67 ± 0,87b
50 4,61 ± 0,04a Fatty Acid Profile
60 13,68 ± 0,94c The fish oil selected for the fatty acid
*Different superscript letters in the same column indicate a profile test was fish oil extracted using a
significant difference (p<0,05)
temperature of 50 oC. This selection was
based on the fact that extraction at 50 oC
Table 5. Total oxidation value of fish oil
produced the best yield, free fatty acids and
Temperature (°C) Total oxidation
anisidine values. The fatty acid profile of
value (mEq/kg)
viscera fish oil showed in Table 6. The fatty
40 61,90 ± 0,48a
acid composition of milkfish viscera fish oil
50 63,53 ± 0,96b
consists of saturated fatty acids (SFA),
60 107,36 ± 0,83c
*Different superscript letters in the same column indicate a
monounsaturated fatty acids (MUFA) and
significant difference (p<0,05) polyunsaturated fatty acids (PUFA). The
fatty acid composition of viscera fish oil was
Anisidine Value dominated by SFA>MUFA>PUFA.
The results showed that temperature SFA are dominated by palmitic acid
had a significant effect (p<0.05) on the with the highest percentage of 31.17%.
anisidine value (Table 4). The lowest Estiasih (2009) stated that palmitic acid is
anisidine value was obtained at the extraction one of the two dominant fatty acids and is
temperature of 50 oC. The anisidine value in most easily obtained in fish bodies with high
all fish oil produced in this study met the

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Fitriani, et. al.

enough levels, exceeding 30% or more in all Table 6. Fatty acid profile of viscera fish oil
types of fish.

Fatty acid Percentage (%)


Capric Acid, C10:0 0,04
Lauric Acid, C12:0 1,60
Myristic Acid, C14:0 2,55
Pentadecanoic Acid, C15:0 0,27
Palmitic Acid, C16:0 31,17
Heptadecanoic acid, C17:0 0,20
Stearic Acid, C18:0 6,59
Arachidic Acid, C20:0 0,20
Heneichocylic Acid, C21:0 0,02
Behenic Acid, C22:0 0,08
Tricosanoic Acid, C23:0 0,02
Total SFA 42,74
Myristolic Acid, C14:1 0,05
Palmitoleic Acid, C16:1 5,42
Cis-10-Heptadecanoic Acid, C17:1 0,23
Elaidic Acid, C18:1n9t 1,14
Oleic Acid, C18:1n9c 23,56
Cis-11-Eicocenoic Acid, C20:1 2,08
Erucic Acid, C22:1n9 0,13
Nervonic Acid, C24:1 0,04
Total MUFA 32,65
Linoleic Acid, C18:2n6c 6,38
Ƴ-Linolenic Acid, C18:3n6 0,15
Linolenic Acid, C18:3n3 0,83
Cis-11,14- Eicosedienoate Acid, C20:2 0,57
Cis-8,11,14- Eikocetrienoate Acid, C20:3n6 1,12
Arachidonic Acid, C20:4n6 0,52
Cis-13,16- Docosadienoate Acid, C22:2 0,03
Cis-5,8,11,14,17- Eicosapentaenoate Acid, C20:5n3 0,74
Cis-4,7,10,13,16,19- Docosahexaenoate Acid, C22:6n3 0,32
Total PUFA 10,66
Total identified fatty acids 86,04
Total unidentified fatty acids 13,96
Total omega-3 1,89
Total omega -6 8,17
Total omega -9 24,83

MUFA are dominated by oleic acid lowering LDL blood cholesterol and
(23.56%) which is included in omega 9 fatty increasing HDL blood cholesterol (Sartika
acids. Oleic acid (MUFA) has a better role 2008).
than omega 3 and omega 6 in lowering blood Omega 3 and omega 6 are included in
cholesterol levels. Oleic acid plays a role in PUFA fatty acids. Omega 6 is dominated by

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Food Scientech Journal 3(2) 2021
Preliminary study of fish oil ..

linoleic acid with a percentage of 6.38%. AOCS [American Oil Chemists Society].
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