Leica DM300

User Manual
Congratulations!

Congratulations on purchasing the

Leica DM300 Compound Microscope. This
model’s exclusive design features and full range
of accessories make it a truly versatile, high
quality-instrument.

Though the reliability and robustness of

Leica microscopes is already practically prover-
bial, they still require a certain degree of care
and attention. Therefore, we recommend that
you read this User Manual. It contains all the
information you need regarding operation,
safety and maintenance. Simply observing a
few guidelines will ensure that even after years
of intensive use, your microscope will continue
to work as smoothly and reliably as on the very
first day.

We wish you the best of success in your work!

Leica DM300 User Manual  2

Contents

Important Notes Using the Leica DM300

Symbols Used in this User Manual 5 Switching on the Microscope 24
Important Safety Notes 6 Selecting an Objective 25
Instructions for Use 8 Preparing for Viewing 26
Health risks and dangers of use 10 Focusing27
Information for the Person Responsible for the Instrument 11 Adjusting the Binocular Tubes 28
Accessories, Maintenance and Repair 12 Adjusting the Eyecups 30
Electrical Data and Ambient Conditions 13 Oil Immersion Technique 31

Assembling the Leica DM300 Care of the Microscope

Unpacking15 Maintenance instructions 34
Identifying your Tube 16 General maintenance 35
Mounting the binocular tube 17
Installing the Monocular Tube 18 Dimensions
Removing and Inserting Objectives 19 Dimensions (all dimensios in mm, DM100 and DM300 identical) 38
Installing the Mirror (optional) 21
Installing the Polarizer Kit (optional) 22

Leica DM300 User Manual  3

Important Notes

Leica DM300 User Manual Important Notes4

Symbols Used in this User Manual

Warning! Safety hazard! Warning of hazardous electrical Voltage Important Information

 This symbol indicates especially impor-

tant information that is mandatory to
 
This symbol indicates information that
must be read and observed.   This symbol indicates additional infor-
mation or explanations that are intended
read and observe. to provide clarity.
Failure to comply can cause the following:
Failure to comply can cause the following: OO Hazards to personnel.

OO Hazards to personnel. OO Instrument malfunctions and damage.

OO Instrument malfunctions and damage.

Leica DM300 User Manual Important Notes5

Important Safety Notes

  Before installing, operating or using

the instrument, it is mandatory to read
this User Manual. In particular, please follow all
Non-intended Use

  Using the instrument in any way other

than that described in this User Manual
"Safety Concept" Brochure
The "Safety Concept" brochure contains addi-
tional safety information regarding service
safety instructions. may result in personal injury or damage to work, requirements and the handling of the
property. Doing so can impair the functions of microscope, the electrical and other accessories

 The instruments and accessories

described in this User Manual have been
the protective measures provided. as well as general safety instructions.

tested for safety and potential hazards.

  The responsible Leica affiliate must be
consulted whenever the instrument is
altered, modified or used in conjunction with
Original Condition
non-Leica components that are outside of the
To maintain the unit in its original condition
scope of this User Manual! Unauthorized altera-
and to ensure safe operation, the user must
tions to the instrument or noncompliant use
follow the instructions and warnings contained
shall void all rights to any warranty claims.
in this User Manual.

Leica DM300 User Manual Important Notes6

Important Safety Notes (continued)

Legal Regulations Disconnecting from the Power Supply

Adhere to general and local regulations relat-
ing to accident prevention and environmental
 
Always position the Leica DM300 so
that you can disconnect it from the
protection. power supply at any time. The power cable
must remain accessible at all times, because
EC Declaration of Conformity the power cable is intended as a power discon-
Electrically operated accessories are nect device.
constructed based on the state of the art of
technology and are provided with an EC Decla-
Plug
ration of Conformity.
 Never install any other plug
(NEMA 5-15P) or unscrew any mechani-
cal components unless expressly instructed to
do so in the instructions.

Leica DM300 User Manual Important Notes7

Instructions for Use

This User Manual describes the Leica DM300 Intended Use Avoid large temperature fluctuations, direct
and includes important instructions related to The Leica DM300 is an optical instrument for sunlight and vibrations. These conditions
operating safety, maintenance and accessories. improving the visibility of objects through can distort measurements and micrographic
magnification and illumination. It is used for images.
Your Leica DM300 includes an interactive observing.
CD-ROM with all relevant User Manuals in In warm and warm-damp climatic zones,
additional languages. Keep it in a safe place, Place of Use the individual components require
and readily accessible to the user. You can also The Leica DM300 microscope may be used only special care in order to prevent the build-up of
download User Manuals and updates from our in closed rooms and must be placed on a solid fungus.
website at www.leica-microsystems.com. surface.

The Leica DM300 can be used at +10 °C to

+40 °C. Protect the microscope from oil, chemi-
cals and extreme humidity. If it is used outdoors,
the microscope must be protected from dust
and moisture. Never use electrical instruments
outdoors and place them at least 10 cm away
from the wall and from flammable substances.

Leica DM300 User Manual Important Notes8

Instructions for Use (continued)

Accessories from third-party Suppliers Transport

You can combine individual system articles with If at all possible, use the original packaging for
articles from external suppliers. Please read the shipping or transporting individual modules.
User Manual and the safety instructions from
the supplier. In order to prevent damage from vibrations,
all moving parts that (according to the User
Integration in third-party products Manual) can be assembled and disassembled
When installing Leica products into third-party by the customer should be packed separately.
products, the manufacturer of the complete
system or its dealer is responsible for follow- Disposal
ing all applicable safety instructions, laws and Disposal must comply with locally applicable
guidelines. laws and regulations.

Leica DM300 User Manual Important Notes9

Health Risks and Dangers of Use

Health Risks The ergonomic optical design and construction Dangers during Use

 Workplaces with microscopes facili-

tate and improve the viewing task, but
they also impose high demands on the eyes
of the Leica microscopes are intended to reduce
the exertion of the user to a minimum.
OO The Leica DM300 may be connected to a

grounded socket only.

OO The Leica DM300 may be used when it is in
and hand muscles of the user. Depending on Danger of Infection proper working condition only.
the duration of uninterrupted work at such a Direct contact with eyepieces can be a
workplace, impaired vision and problems with potential transmission path for bacterial
LED Class 1 /LED 1 Laser Product
the musculoskeletal system may occur. For this and viral infections of the eye.
The illumination meets laser class 1 require-
reason, appropriate measures for reduction of

  The risk can be minimized by the use of ments in accordance with IEC 60825-1 "Safety
the workload must be taken:
personal eyecups. of Laser devices".
OO Optimum workplace layout
OO Frequent changes of activity Caution: Hazardous radiation may occur if
controls, adjustment jigs, or procedures other
OO Thorough training of the personnel, giving
than those outlined in this User Manual are
consideration to ergonomic and organiza-
used!
tional aspects

Class 1 LED Class 1

LED Product

Leica DM300 User Manual Important Notes10

Information for the Person Responsible for the Instrument

Information for the Person Responsible for OO Inform your Leica representative or Leica OO After service work or technical modifica-
the Instrument Microsystems (Schweiz) AG, Industry tions, the device must be readjusted in
OO Ensure that the Leica DM300 is used only by Division, 9435 Heerbrugg, Switzerland, accordance with our technical specifica-
persons qualified to do so. immediately of any product defect that tions.
OO Ensure that this User Manual is always avail- could potentially cause injury or harm. OO If the unit is modified or serviced by unau-
able at the place where the microscope is OO If you use accessories from other manu- thorized persons, is improperly maintained
in use. facturers with the microscope, make sure (as long as maintenance was not carried
OO Carry out regular inspections to make that these manufacturers confirm that out by Leica Microsystems), or is handled
certain that the authorized users are adher- the combination is safe to use. Follow the improperly, Leica Microsystems will not
ing to safety requirements. instructions in the User Manual for those accept any liability.
accessories. OO The building’s electrical wiring must
OO When instructing new users, do so thor-
oughly and explain the meanings of the OO Modifications to or service on the micro- comply with the relevant national stand-
warning signs and messages. scope may be carried out only by techni- ard; in other words, we recommend using a
cians who are explicitly authorized by Leica current-operated ELCB system (RCB).
OO Allocate responsibilities for commission-
to do so.
ing, operation and maintenance. Monitor
compliance with this. OO Only original Leica replacement parts may
be used in servicing the product.
OO Do not use the Leica DM300 unless it is in
perfect condition.

Leica DM300 User Manual Important Notes11

Accessories, Maintenance and Repair

Accessories Maintenance Repairs and Service Work

Only the following accessories may be used OO The Leica DM300 microscope is basically OO Only original Leica Microsystems spare

with the Leica DM300: maintenance-free. To ensure that it always parts may be used.
operates safely and reliably, we recommend OO Before opening the instruments, switch off
OO The Leica Microsystems accessories that you take the precaution of contacting the power and unplug the power cable.
described in this User Manual. the responsible service organization.
OO Avoid contact with powered electrical
OO Other accessories, provided that these have OO We recommend concluding a service circuits, which can lead to injury.
been expressly approved for use by Leica contract with Leica Microsystems.
Microsystems with regard to safety tech-
Service Address
nology.
In case of problems, please contact us as
follows:

Contact address
Leica Microsystems (Schweiz) AG
Industry Division
Max Schmidheiny Strasse 201
CH-9435 Heerbrugg (Switzerland)

Leica DM300 User Manual Important Notes12

Electrical Data and Ambient Conditions

Fuse Replacement

 Unplug the instrument before changing any fuses. The Leica

DM300 has two fuses, which are located behind the power cord
  In order to maintain this condition and to ensure safe operation,
the user must follow the instructions and warnings contained in
this instruction manual.
receptacle.

  Only use fuses of the following type: 5×20 mm, 1.0 A/250 V, high
breaking capacity (# 13RFAG30003)
Environment
Temperature for use +10 °C … +40 °C
Storage temperature -20 °C … +52 °C
Electrical Data +50 °F to +104 °F
Input: 100–240 V, 50/60 Hz, 5 W (3 W LED) Manipulation shock 25 mm on 50 mm hard wood
General safety notes Transport shock (unpacked) 100 g / 6 ms
This instrument of safety class 1 has been built and tested in accordance Transport shock (packed) 800 mm free fall
with the following safety requirements for electrical equipment for measu-
Transport vibrations (unpacked) 5–200 Hz / 1.5 g
rement, control, and laboratory use:
Air pressure during use and storage 500–1060 mBar
Humidity during use and storage 20–90 %
EN 61010-1: 2002-08 EN 55011: 2007+A2: 2010-05
Installation Category II (Overvoltage Category)
EN 61010-2: 2008-06 EN 60825-1: 2008-05
Pollution degree 2
IEC 61010-1: 2010-06 IEC 60825-1: 2007-03
EN 61326-1: 2006-10 LED Class 1
EN 61326-2-6: 2006-10

Leica DM300 User Manual Important Notes13

Assembling the Leica DM300

Leica DM300 User Manual Assembling the Leica DM30014

Unpacking

OO Carefully remove the microscope and any

components from the packaging.
OO Check the components against the planned
configuration.
OO Optional items such as contrast accessories,
camera adapters, cameras, and carrying
cases are not shipped as part of the stan-
dard equipment. These items are delivered
in separate packages.
OO Please do not discard any of the packing
materials. They should be used for safely
storing and transporting the instrument
should the need arise.

Leica DM300 User Manual Assembling the Leica DM30015

Identifying your Tube

Identifying your Tube

  There are three types of viewing tubes.

Identify which viewing tube you have:

Assembly

  Installation of the tube is described on

page 17 using the binocular "EZTube."
Other tubes, however, are installed exactly the Type 1: Monocular "EZTube" Type 2: Binocular "EZTube"
same way.

Orientation

 All tubes are connected via a dovetail

and can be installed in any orientation.

Type 3: Open binocular tube

Leica DM300 User Manual Assembling the Leica DM30016

Mounting the Binocular Tube

Tools used Assembly 3. Turn the tube in the direction you desire.
OO Allen key 1. Loosen the setscrews at the left and right
4. Tighten the set screws on the left and right,
on the stand using the Allen key provided.
without applying too much force.

  This User Manual shows the binocular

tube. The installation of the monocu-
lar tube is shown on the next page. However,
handling the microscope is the same for both.
2. Insert the dovetail of the binocular tube
Eyepieces into the mount.

  The eyepieces are already integrated in

the Leica "EZTube" and preset; for this
reason, the eyepieces do not require adjust-
ment or assembly.

Leica DM300 User Manual Assembling the Leica DM30017

Installing the Monocular Tube

Tools used Assembly 3. Turn the tube in the direction you desire.
OO Allen key 1. Loosen the setscrews (on top of the stand)
4. Tighten the set screws on the left and right,
using the Allen key provided.
without applying too much force.

  This User Manual shows the binocular

tube from this point on. However, hand-
ling the microscope is the same for both. Possi-
ble deviations are mentioned on the respective
page. 2. Insert the dovetail of the monocular tube
into the mount.
Eyepieces

  The eyepieces are already integrated in

the Leica "EZTube" and preset; for this
reason, the eyepieces do not require adjust-
ment or assembly.

Leica DM300 User Manual Assembling the Leica DM30018

Removing and Inserting Objectives

  The objectives are already installed

on the Leica DM300 at delivery. The
following description shows how to (un)install
 Always turn the objective nosepiece by
its knurled ring. Never turn the objective
nosepiece by using an objective as a handle.
The proper Sequence

  When inserting the objectives, you can

begin with any available space on the
the objectives if they have to be removed for microscope. Continuation of the sequence,
cleaning or replaced by other objectives. however, has to be selected such that the
Removing Objectives
magnification always increases when turning

  Your Leica DM300 is delivered with

objectives with magnification levels 4×,
10× and 40×, possibly also with a 100× objec-
1. Unscrew the objectives to remove them.
clockwise:

tive. Otherwise, this can be retrofitted at any 100×

time.

4× 40×

10×

Continued on next page.

Leica DM300 User Manual Assembling the Leica DM30019

Removing and Inserting Objectives (continued)

Inserting Objectives
1. Screw the 4× objective into any available
space on the objective nosepiece.

2. Turn the objective nosepiece clockwise one

unit.

3. Screw in the next-largest objective.

4. Repeat steps 2 and 3 until all objectives

have been installed.

Leica DM300 User Manual Assembling the Leica DM30020

Installing the Mirror (optional)

The mirror is available as an optional Installing the Mirror Removing the Mirror
accessory. By means of this, the ambient 1. Position the microscope at a well-lit loca- 1. Simply pull the mirror out of the holder.
light can be used for illuminating the sample if tion, for example before a window.
no power source is available for the built-in LED
2. Press the mirror onto the mount of the illu-
illuminator.
minator.

 The mirror is equipped with a flat and

a concave surface. Try them out to see
which side works better.

3. Look through the eyepieces and align the

mirror so that it works best.

Leica DM300 User Manual Assembling the Leica DM30021

Installing the Polarizer Kit (optional)

  The polarizer kit is not included in the

delivery package, but available as an
optional accessory.
Assembly
1. If you have already installed the tube,
remove it (see page 17).
4. Place the polarizer onto the aperture of the
illuminator. The handle must come to rest
in the aperture with the white arrow poin-
ting upwards.
2. Place the analyzer with the flat side facing
down into the beam path. The two screws
have to rest in the two existing holes.

3. Install the tube.

5. Turn the polarizer to align it. The arrows

indicate the current orientation.

Leica DM300 User Manual Assembling the Leica DM30022

Using the Leica DM300

Leica DM300 User Manual Using the Leica DM30023

Switching on the Microscope

 For safety reasons, the power cable

is grounded via the microscope base.
Startup Procedure
1. Attach the power cable to the rear side of
3. Switch on the microscope using the toggle
switch on the rear side of the stand.
Never use an adapter between the power cable the microscope.
and the power supply; that would render the
2. Set the illumination to the lowest setting
grounding ineffective.
using the controller on the bottom left of
the stand.
Work Surface

 Always use the microscope on a hard,

stable surface.

Leica DM300 User Manual Using the Leica DM30024

Selecting an Objective

Using the Condenser Selecting an Objective 2. Rotate the condenser diaphragm below the

  The condenser is furnished with an iris

diaphragm, which has to be adjusted to
match the effective numerical aperture of the
1. Rotate the desired objective over the
sample.
specimen stage so that the displayed value
matches the selected objective.

individual objectives.

Leica DM300 User Manual Using the Leica DM30025

Preparing for Viewing

Preparation 2. Insert the specimen slide and slowly release 3. Using the X/Y control, position the speci-
1. Open the specimen holder using the the tension lever. men slide such that a part of the specimen
tension lever. is under the objective used.

Leica DM300 User Manual Using the Leica DM30026

Focusing

Focusing 2. Move the specimen stage upwards as far as 3. Look into the eyepieces and adjust the illu-
1. Use the knurled ring to rotate the objective it will go using the coarse adjustment knob. mination to a comfortable intensity.
nosepiece in such a way that the objec-
4. Bring the specimen into focus using the
tive with the lowest magnification level is
fine adjustment knob.
turned into the working position.

Leica DM300 User Manual Using the Leica DM30027

Adjusting the Binocular Tubes

1. Adjust the tubes to your interpupillary

distance. Fold or unfold the eyetubes to
decrease or increase the distance between
  The viewing tubes maintain a constant
tube length for all interpupillary settings.
This means that a change of interpupillary
Open Binocular Tube
If you are using an open standard tube, you
need to make some adjustments.
the eyepieces until you see one illuminated distance does not affect parfocality, magnifica-
circle. tion, or calibrations that depend on magnifica- 1. Set the focusing eyetube (left side) to "0".
tion.

Leica EZ tubes
If you are using a Leica EZ viewing tube, which
already has the eyepieces integrated with the
eyetubes, no additional adjustments are neces-
sary. Be sure you are wearing your eyeglasses or
contact lenses.

Continued on next page

 

Leica DM300 User Manual Using the Leica DM30028

Adjusting the Binocular Tubes (continued)

 corrective
If you are comfortable wearing your 5. Now, switch to an objective with a higher
lenses (contact lenses or magnification level (no oil objective) and
eyeglasses) for microscope viewing, leave them bring the specimen into focus while looking
on and your adjustments will be minimal. through the eyepieces with both eyes.

 magnification
2. Bring the specimen into focus using 10× The depth of field is lower for higher
magnification and the fine adjustment levels. Thus, you will
knob while looking through only the right notice that if you switch to low magnification
eyepiece. To help focus, cover or close your after bringing the microscope into focus with
left eye. great magnification, a minor adjustment of the
fine focus may be required. Continue with the
3. Now look with your left eye through the
"Oil immersion" section on page 31.
left eyetube (focusing eyepiece). Now focus
using the focus function of the eyetube.

  When doing so, do not change the

height of the specimen stage.

4. Rotate the knurled ring on the left eyetube,

until the specimen is sharply focused for
the left eye. This corrects for any vision
differences between your right eye and left
eye.

Leica DM300 User Manual Using the Leica DM30029

Adjusting the Eyecups

  If you wear eyeglasses for microscope

viewing, keep the rubber eyecups folded
down. If you do not wear eyeglasses, you may
Potential Sources of Infection

  Direct contact with eyepieces is a poten-

tial transmission method for bacterial
find it useful to unfold the rubber eyecups in and viral infections of the eye. The risk can be
order to help block out ambient room light. minimized by the use of individual eyecups.
Eyecups can be ordered separately. Please
contact your Leica partner.

Leica DM300 User Manual Using the Leica DM30030

Oil Immersion Technique

Oil Immersion Technique 3. Add a drop of immersion oil from Leica 5. Slowly move the specimen stage upwards
1. Search for the area on the specimen slide to the area of the specimen slide you are using the coarse adjustment knob until
that you want to study. studying. the oil drop on the slide comes into direct
contact with the lens of the oil immersion
2. Move the specimen stage down to the
objective.
lowest position using the coarse adjust-
ment knob.

4. Rotate the 100× objective to the working

position.
6. Hold the knurled ring on the objective
nosepiece and swing the objective back
and forth to eliminate air bubbles. Then
bring the oil objective into the final posi-
tion, so that the oil drop is between the
front lens of the objective and the speci-
men slide.

Leica DM300 User Manual Using the Leica DM30031

Oil Immersion Technique (continued)

7. Look through the microscope and slowly

rotate the fine adjustment knob, so that the
specimen stage moves upwards until the
specimen is in focus.

8. If you are finished w orking w ith t he o il

immersion objective, clean the front side
of the objective, the specimen slide, and all
other surfaces that have come into contact
with the oil, following the instructions in
the "Care of the Microscope" section on
page 33.

Leica DM300 User Manual Using the Leica DM300 32

Care of the Microscope

Leica DM300 User Manual Care of the Microscope33

Maintenance instructions

General Notes Cleaning coated Parts and plastic Parts

OO Protect the microscope from moisture, OO Dust and dirt particles should be removed

fumes and acids and from alkaline, caustic with a soft brush or lint-free cotton cloth.
and corrosive materials. Do not store chem- OO Remove coarse debris with a moistened
icals in the vicinity. disposable cloth.
OO Protect the microscope from oil and grease. OO Acetone, xylene or nitro-containing thin-
Never grease or oil mechanical parts or slid- ners must NOT be used.
ing surfaces.
OO Never use chemicals to clean colored
OO Follow the instructions of the disinfectant surfaces or accessories with rubberized
manufacturer. parts. This could damage the surfaces,
OO We recommend concluding a service and specimens could be contaminated by
contract with Leica Service. abraded particles.

Leica DM300 User Manual Care of the Microscope34

General Maintenance

General OO Keep all optical components clean. Clean- OO Optical surfaces should be cleaned using a

 Always use both hands to carry the

microscope.
liness is important for maintaining good
optical performance.
lint-free cloth with a commercially available
glass cleaner.
OO When the microscope is not in use, it OO Use solvent only very sparingly, so that it
should always be put away with protection does not flow out over the lens. No part
against dust. of the microscope is quite so vulnerable to
OO If any optical surface becomes coated with collecting dirt, dust, and oil as the front lens
dust or dirt, clean the surface by blowing it of the objective. Whenever you encounter
off with a syringe or brushing it off with a lack of contrast, cloudiness or poor defini-
camel hair brush before attempting to wipe tion, carefully check the condition of the
the surface clean. front lens with a magnifier.

Leica DM300 User Manual Care of the Microscope35

General Maintenance (continued)

OO The lower magnification objectives have Illumination

fairly large front lenses and can be cleaned OO The Leica DM300 uses an LED illuminator.

with a cloth or lens tissue wrapped around a Therefore, no lamp changing is required for
finger and moistened with window cleaner. the entire life of the microscope.
OO Cleaning 40× and 100× objectives requires
more care. To attain the typical flatness for
objectives with higher magnification, the
objective has a small concave front lens
with a very small radius and slight curva-
ture. The surface of this front lens can be
readily cleaned with a toothpick covered
with a cotton tip, or with a small cotton
swab. Wipe the front lens lightly without
applying undue force or scrubbing action.
Make sure that the cotton tip contacts the
concave lens surface. Check the objective
with a magnifier after cleaning.

Leica DM300 User Manual Care of the Microscope36

Dimensions

Leica DM300 User Manual Dimensions37

Dimensions (all Dimensions in mm, DM100 and DM300 identical)

OO side view with binocular tube OO front view with binocular tube OO side view with binocular tube 180° rotated
for storage

Leica DM300 User Manual Dimensions38

Dimensions (all Dimensions in mm, DM100 and DM300 identical)

OO side view with monocular tube OO front view with monocular tube OO side view with monocular tube 180°
rotated for storage

Leica DM300 User Manual Dimensions39

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