Operation Manual Number : LAC-3011VE(3)

Edited : 28.Aug.2009
Revised : 26.October.2011
15. December.2011
June 2017

Bench Top Autoclave / Sterilizer

Model LAC-3011V/3021V
With Pre-Vacuum & Dry System

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 Thank you very much for purchasing DAIHAN LABTECH CO., LTD products.
In order to use the product correctly and most appropriately, please carefully read and fully understand instructions in
this manual and keep it in a convenient location for further reference.
In order to protect the human life and property, please pay attention to the following sings.

Warning
WARNING
This mark indicates precautions that must be taken if there is danger of electric shock, fire, etc.
which could result in loss of life or injury

Caution
CAUTION
This mark indicates that if these precautions and operation procedures are not taken, damage
to the equipment may result.

Note
This mark indicates that all precautions should be taken for safe usage.

Hot Surface

HOT This mark indicates that burning injury by hot surface may result.

Explosive
This mark indicates that explosion by high pressure may result.

WARNING

 This equipment must be used in accordance with the specifications to prevent fire or damage to equipment.
 Equipment not suitable for use in the presence of a flammable anesthetic mixture with air or with oxygen or nitrous
oxide.
 Do not touch high-voltage connections such as power supply terminals, etc. to avoid electric shock.
 DAIHAN LABTECH is not responsible if this equipment is repaired, modified of disassembled by other than
factory-approved personnel.
 Clogging lines, Equipment malfunction of Failure in the steam supply may cause the autoclave chamber to fill with
scalding water. If the water leaks from any part of the autoclave, do not open the chamber door burns from scalding
water may otherwise result.
 Potential exposure to infectious agents through either airborne, droplet or contact transmission.
 Do not open the chamber door at any time until the pressure gauge indicating ‘0’.

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 CAUTION

 All precautions described in this manual should be taken to avoid damage to the equipment.
 All wiring must be in accordance with local codes and regulations.
 Prevent metal fragments or lead wire scraps from falling inside equipment case to avoid electric shock, fire of
malfunction.
 For proper operation of this equipment, provide adequate ventilation for heat dispensation.
 Turn off the power supply before cleaning the equipment.
 Do not use a volatile solvent such as paint thinner to clean the equipment. Deformation of discoloration will occur.
Use a soft, dry cloth to remove stains from the equipment.
 To avoid damage to equipment display, do not rub with an abrasive material or Press front panel with a hard object.
 Do not refill the water in the reservoir in operation.
 When processing the Liquids, use only flasks and self-venting automatic sealing stoppers recommended by the
manufacturer. This will help to prevent liquid in the glass containers from rupturing.
 Waste that contains bleach may harm an autoclave.
 Carefully prepare items for autoclaving loosely cover or cap containers to avoid over-pressurization.
 Keep loads appropriately – overloading hinder the steam penetration.
 Bags should be opened, contained within the stainless steel tray or basket.
 Place an autoclave on the plat and leveled surface.
 Be sure that connect the drain and steam ventilation valve to the drain sink.
 Use distilled water only to protect the heater and sensor.
 Change the water in the chamber frequently to prevent contamination and odor

HOT

 Do not touch the part of the top of the autoclave or the cover.
- Caused by contact with the outside of autoclave
- Caused by contact when removing autoclaved items
- Caused by contact with pressurized steam
 Keep away from the autoclave at least 50 ㎝ during operation to prevent burning caused by leakage of the steam.
 After ventilated completely, open the chamber door about one centimeter and allow ten minutes to let the load cool
before removing tray.
 All steel parts of an autoclave are hot. Do not touch during operation or sterilization process.

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 The figures, diagrams and numeric values used in this manual are only for the purpose of illustration.
 DAIHAN LABTECH is not responsible for any damage or injury that is caused as a result of using this equipment,
equipment failure of indirect damage.
 No portion of this document may be reprinted, copied, stored, and retrieved through any electronic, optical or other
means without prior written approval from DAIHAN LABTECH.
 Do not remove the ground prong(third pin) from the power cord, or use an ungrounded adapter. The autoclave
requires a 3-wire receptacle. If one is not available, consult with an electrician for installation.
 Your autoclaves are 220/230VAC 50/60HZ SINGLE PHASE.
 To reduce the risk of electric shock, do not open the body, no user serviceable parts inside.

 Never sterilize the nitrocellulose tubes – they can explode!

 Do not put flammable, volatile or explosive materials inside of the chamber.

Safety Sticker for safety

a. Warning of hot surface

 This mark indicates that burning injury by hot surface may result.
 Do not touch this surface. This area can make burning injury.
 Do not take off safety cover during operation.
 Do repair or inspect after cooling down the unit.

Size

27mm

30mm

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b. Warning of electric shock

Size

27mm

30mm
c. Caution

The cover of unit has to be fixed with screw bolts during operation.
! So it needs continuous management to prevent from unexpected accidents.
.

It has frame structure but it can be damaged with weight over 50kg on top.
! So don’t put any kind of heavy products on top of unit.

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1. Tables of Contents

1. Tables of Contents ···································································································6

2. Beginning ·············································································································6
3. Product Overview····································································································7
4. Product Specifications ······························································································8
5. Names and Functions································································································9
6. Installation ·········································································································· 13
7. Operation ··········································································································· 14
8. Trouble Shooting ·································································································· 19
9. Maintenance ········································································································ 20
10. Limited Warranty and statements ················································································ 21
APPENDIX Ⅰ(P&I schematic) ······················································································· 22
APPENDIX Ⅱ(Electric circuit) ······················································································· 23
APPENDIX Ⅲ(Inspection Log) ······················································································· 24
APPENDIX Ⅳ(Use List) ······························································································· 25

2. Beginning

Dear First Time User,

Your Autoclave/Sterilizer using high temperature and pressure steam.
If you are not trained for Autoclave usage or not familiar with Autoclave, please do not operate before you are
trained or completely read this manual and understand about your Autoclave for your health and safety and to
prevent personal injury.
If you do not understand, please contact your laboratory supervisor or technician for further training cycle or
advise.

Autoclaves are such a familiar feature in many laboratories that it is easy to forget what hazards they can pose. The
autoclave’s job is to render its contents sterile, or free of any living organisms. If it fails to do so, serious health hazards
can result. The hot, pressurized steam (135℃, 2.65 kg/㎠ in gauge) that autoclaves generate to do this job makes them
serious burn hazards as well. And, because conditions created inside steam autoclaves are so extreme, autoclaves can
easily malfunction if they are not carefully.
Before using DAIHAN LabTech LAC-Series autoclave at first, read and understand the operating manual thoroughly.
If you have not kept the manual, contact a local distributor or us directly to have a copy.

DAIHAN LABTECH CO., LTD. IS NOT RESPONSIBLE FOR ANY DAMAGE OF

PROPERTY OR PERSONAL INJURY FOR ABUSE OR USAGE FOR WRONG PURPOSE
OF AUTOCLAVE.
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3. Product Overview
 Class Ι equipment
 Ordinary equipment
 Continuous operation

 Intelligence self auto-tuned PID controller

The unique LabTech controller computes the PID parameter every sterilization process and it stores the optimized
parameter at the EPROM memory automatically.
It performs high precision temperature control at any circumstance.
It can be stabilized more and more when used.

 Full automatic operation system

Easy operation with full automatic system by micro-processor, auto-water supply and ejection.

 Automatic power cut-off safety system

When the malfunction and errors occur, it can stop working with automatic ventilation process for maximum
safety.

 Self-diagnostic system
Self-test diagnose whether the function is good. When the malfunction occurs in the operating, it sounds and
display error or error number and stop working automatically at the same time.

 Safety system
Over pressure release valve prevents over pressure in the chamber.
Over heating & over temperature protector.
Low water protection device
Leakage & over current circuit breaker
Normal open solenoid valve makes equal the pressure of chamber and the atmosphere.

 Easy Maintenance without Disassembly

Pressing a button, could check and remove the obstacle in the solenoid valve.

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4. Product Specifications

Model LAC-3011V LAC-3021V

Dimensions Overall Size (mm) W480xD720xH400 W530xD780xH420

Chamber (mm) 200x355(H) 250x455(H)
Capacity 11 Liters 22Liters
Reservoir Capacity 5 Liters
Power Consumption 1.5 KW+600W ( 2 heaters ) 2.0KW+600W ( 2 heaters)
Sterilization Temperature 105.0 ℃ ∼ 135.0 ℃
Pressure MAX. 0.22 ㎫ (2.16 kgf/㎠)
Dry System Post vacuum to remove steam vapor / 1 – 60 min
Chamber Stainless Steel (STS316 Option)
Material
Exterior Epoxy Resin Powder Coated Steel ( Option: stainless steel with coating)
Door One touch hinged door / Inter lock door locking system
Controller 3 Pre-Programmed and 2 user programmable micro-processor
Operation Mode 5 selectable mode
LED Display (Temperature , Timer , Program Cycle )
Display
LED Indicator ( Water, Heat, Sterilization, Dry, End cycle
Timer 0 ∼60 min(Sterilization & Dry time)
PC Communication RS-232C Interface(Option)

Over pressure release safety valve

Over heating and over temperature protector
Door open sensing & power cut-off
Safety
Safety door interlock
Low water prevention device
Leakage & over current circuit breaker

Standard Tray Stainless Steel 2 or 3 ea

Electric Supply AC 110V, 60HZ or AC 220/230V, 50/60 Hz

* The above mentioned articles will be able to alter without previous notice to customer for the purpose of more
high performance.

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5. Names and Functions
5.1 Part Names

① FRONT VIEW

Main Controller

Door Cover

Door Lock Handle Rubber adjustable Leg

② FRONT VIEW - DOOR OPEN

Front Panel
Tray & Heater Cover

Door

Main Power Switch

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③ LEFT VIEW – UNCOVERED

Vacuum Pump
with S/V

Earth Leakage Circuit Breaker

④ RIGHT VIEW - UNCOVERED

Control Panel Reservoi

r

Over Temperature Protector

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⑤ REAR VIEW - UNCOVERED

Reservoir Injection
Solenoid Valve/NO
Air filter

Solenoid Valve/NC

Drain Hose

⑥ REAR VIEW

Power cable

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5.2 Main Controller
▪ Pressure Gauge
ᆞ Displays the pressure in the chamber.

▪ Display
① Reservoir Level Indication LED[LOW(Red), MIDDLE & FULL(Green)]
ᆞ Indicates the water level in the reservoir.
ᆞ Lights the Middle & Full LED simultaneously (Full).
② Temperature Display
ᆞ Displays the temperature in the chamber.
ᆞ Displays the Error Code provided that the error occurs.
③ Process Indication LED[Green]
ᆞ Lights when the process has proceeded.
ᆞ Blinks when the process proceeds.
④ Remain Time Display
ᆞ Displays the remaining time of the sterilization or dry.

▪ Control Button
1. Solid Cycle Select Button(Indication LED)
2. Liquid Cycle Select Button(Indication LED)
3. Turbo Cycle Select Button(Indication LED)
4. User Cycle Select Button(Indication LED)
5. Manual Cycle Select Button(Indication LED)
6. Up Button
7. Down Button
8. Enter & Door Button
9. START/STOP Button(Indication LED)

▪ Earth Leakage Circuit Breaker(Left panel)

ᆞ Provides Leakage & Over current limit.

▪ Main Power Switch

ᆞ I = ON O = OFF

The main voltage must correspond with the voltage given on the name-plate.
Place Incubator on the flat and level surface.
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6. Installation
In order to use the sterilizer correctly and most appropriately, please observe in the following statement.

 For proper operation of this equipment, provide adequate ventilation for heat dispensation.
ᆞ The rear & side parts of this equipment should be 15 ㎝ apart from the wall.

 Place an autoclave on the plat and leveled surface.

ᆞ The incline of the sterilizer affects the quantity of water injected in the chamber.

 Be sure that connect the drain and steam ventilation valve to the drain sink.
ᆞ If not, user may be burning injury by the steam or hot water ejection.

 Use distilled water only to protect the heater and sensor.

ᆞ The hard water may give rise to the shortening of heater life, to trouble of the equipment.

 The sterilizer requires a 3-wire receptacle. If one is not available, consult with an electrician for installation.

 Do not use the same outlet for the other instrument simultaneously.

 Requirement of using this sterilizer

ᆞ Door opening
- Do not open the chamber door at any time until the pressure gauge indicating ‘0’.
- Do not make the face or hands close to the upper part of the door.
ᆞ Door closing
- User should close the door certainly, or not, may occurring the error with “door” message and sound.
ᆞ Water Supplementing
- Use distilled water only to protect the heater and sensor.
- The hard water may give rise to the shortening of heater life, to trouble of the equipment.
- Do not supplement the reservoir in operation. May occur overflow at the reservoir during ventilation.
ᆞ Water replacing
- Replace the water frequently(1time/2weeks recommended).
- Using the hard water especially, replace the water more frequently.
ᆞ Sterilized goods loading
- Place the goods vertically(reduce a contact surface of goods as possible).
ᆞ Do not place the goods on the external cover or injector of the reservoir
ᆞ In order to use the sterilizer continually, use the sterilizer after cooled at least ten minutes.

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7. Operation

7.1 Operating
1) Turn the circuit breaker ON which located on the left panel.

2) Turn the main power switch ON located on the main controller.

3) Check up the reservoir water level.

- If press the START Button under Low level alert LED on, may occur the alarm(Er10).
- Please, remove moisture from the chamber level sensor fully.
 Leave the equipment around after sterilization, may occur moisture on the inner side of the chamber.
 May occur the over-heating error(Er02) by malfunction of the chamber level sensor.

4) Place the tray in the chamber that contained the sterilization material.

5) By pressing the ENTER/DOOR Button, can shift the door lock Handle for 10 sec approximately.
- Can not shift the door lock Handle after 10 sec.
- The door is open in case that the Handle direction is the straight horizontal.
- The door is close in case that the Handle direction is the straight vertical.
- Easy door close method
 Place the door lock Handle on straight horizontal.
 Push the door lock Handle in order that place the door parallel with the front panel.
 Push the ENTER/DOOR Button.
 Turn the door lock Handle on straight horizontal.
- If the door is not closed certainly, please repeat the step 5.

6) Select the appropriate cycle correspond to the sterilization material.

CYCLE STER. TEMP. (℃) STER. TIME (M) DRY TIME (M)
SOLID 132 10 20
LIQUID 121 30 0
TURBO 132 5 10
USER 105 ∼ 135 5 ∼ 60 1 ∼ 60
MANUAL 105 ∼ 135 5 ∼ 60 1 ∼ 60
※ Refer to the following table for User & Manual Cycle.

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USER CYCLE  Frequently set value is programmed by user purpose.
① Press the User Cycle Select Button.
② Displays the inner temperature in the chamber.
③ If changing the sterilization temperature and time, press the User Cycle Select Button twice.
④ Blinks the sterilization temperature display brightly.
- Press the UP/DOWN(▲/▼) Button to set what want(range 105 ~ 135 ℃).  press the Enter Button.
⑤ Blinks the sterilization time display brightly and lights the sterilization LED.
- Press the UP/DOWN(▲/▼) Button to set what want(range 5 ~ 60 min).  press the Enter Button.
⑥ Blinks the dry time display brightly and lights the dry LED.
- Press the UP/DOWN(▲/▼) Button to set what want(range 1 ~ 60 min).
⑦ Press the Enter Button.
- If press the START Button in ② step, the autoclave will be operated by previous setting value.
MANUAL CYCLE  When the instant set value is required
① Press the Manual Cycle Select Button.
② Blinks the sterilization temperature display brightly.
- Press the UP/DOWN(▲/▼) Button to set what want(range 105 ~ 135 ℃).  press the Enter Button.
③ Blinks the sterilization time display brightly and lights the sterilization LED.
- Press the UP/DOWN(▲/▼) Button to set what want(range 5 ~ 60 min).  press the Enter Button.
④ Blinks the dry time display brightly and lights the dry LED.
- Press the UP/DOWN(▲/▼) Button to set what want(range 1 ~ 60 min).  press the Enter Button.
⑤ Displays the inner temperature in the chamber.
- If press the Manual Cycle Select Button once, can move the step ②
7) After selecting the cycle, press the START Button.
- The selected cycle starts.

8) Filling.
- Blinks the fill LED and the Filling process starts.
- Supply the water in the reservoir to the chamber automatically(within 5 min).

9) Heating
- When the Filling process ends, blinks the heat LED and the Heating process starts.

10) Sterilization
- When the inner temperature in the chamber reaches the set temperature, blinks the Sterilization LED and
the Sterilization process starts.
- Displays the remaining time on the Remain Time Display

11) Ventilation
- When the Sterilization process ends, blinks the Ventilation LED and the Ventilation process starts.
- Displays the remaining time on the Remain Time Display.

12) Pre-Vacuum and Dry

- When the Ventilation process ends, blinks the Dry LED and the Dry process starts.
- Displays the remaining time on the Remain Time Display

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 13) End
- When the Dry process ends, blinks the End LED and buzzers 15 sec approximately.
- If you want to stop the buzzer at this time, press the START/STOP button.

14) Press the ENTER/DOOR Button. And then, a user may open the door and pull out the sterilization material.
- You can not open the door by the time the inner temperature in the chamber goes down under the 101 ℃.
- Before opening the door, a user should ensure that the pressure gauge indicate the ‘0’surely.

☞ The 7 ∼ 14 process is the automatic operation.

☞ When you want to stop the process in case of error and/or malfunction, press the STOP Button.
And then, the equipment can be stopped automatically after the Ventilation is finished.
☞ If you want to clear the error message, press the Enter button.
☞ In case of the above cycle, the water in the chamber may not be exhausted completely(It is not malfunction).

15) Continuous operation.

▪ potential electromagnetic waves.
This equipment has been tested and found to comply with the limits for medical devices in IEC 601-1-2 : 1994.
These limits are designed to provide reasonable protection against harmful interference in a typical medical
installation.
This equipment generates uses and can radiate radio energy and, if not installed and used in accordance with the
instructions, may cause harmful interference to other devices in the vicinity.
However, there is no guarantee that interference will not occur in a particular installation. It this equipment does
cause harmful interference to other devices, which can be determined by turning the equipment off and on, the user
is encouraged to try to correct the interference by one or more of the following measures;
- Reorient or relocate the receiving device.
- Increase the separation between the equipment.
- Connect the equipment into an outlet on a circuit different from that to which the other device(s) are
Connected – Consult the manufacturer or field service technician for help.

16) Principle of supplying water to chamber

- difference of pressure
* sequence of operation
- vacuum –water sol valve open after 9min– distilled water – heating – sterilization –
stop heating - water vent sol open – water out & steam out – water vent sol close – steam vent sol open –
vacuum pump on -dry – end

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17 )Environment for operation
▪ Transport & Storage condition

- The direction has to be as arrows. Avoid the moisture. Fragile. Do not use hook.
- Temperature of shipping: 0℃ ~ +70℃
- Moisture of shipping : relative humidity 0%~ 95%
- Pressure of atmosphere: 760mmHg

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 7.2 Maintenance Mode
To enter the Engineer Mode, Simply follow the next 3 steps.
First, turn off the system.
Second, in the state of pushing START/STOP SW, turn on the system
Third, remain in that status for 6 sec approximately.

① Each button operates different relays – output test

SOLID BUTTON : Heater 1 Relay on/off + LED 2 on/off

LIQUID BUTTON : Pump/Dry Relay on/off + LED 2 on/off
TURBO BUTTON : Water Fill Relay on/off + LED 2 on/off
USER BUTTON : VENT Relay on/off + LED 2 on/off
MAUNUAL BUTTON : HEATER 2 Relay on/off + LED 2 on/off
INC BUTTON : Water Vent Relay on/off + LED 2 on/off
DEC BUTTON : AIR FILTER Relay on/off + LED 2 on/off
ENTER BUTTON : Door Open Relay on/off + LED 1 on/off

② Input test

By looking the Left digit on the Remain Time Display, you can perceive each Sensor Status.
Each display status is as following.
- If there is a malfunction, apply for a service.
a

f b
g

e c
h
d

Remain Time Display

Left Digit

The status of target correspond with the status of the Remain Time Display
bit Position Target Status(bit ON) Note
SEG bit-a Water sensor Good Reservoir Level LED also
*
SEG bit-b N/A - -
SEG bit-c Water level sensor ( Low ) Connection Link up = ON
SEG bit-d Water level sensor ( high ) Connection Link up = ON
*
SEG bit-e Chamber Thermostat(N/A ) connection Link up = ON
SEG bit-f Door sensor Close -
*
SEG bit-g N/A - -
*
SEG bit-h N/A - -
N/A*: Not Applicable

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 8. Trouble Shooting
A. General malfunction
Display Cause Countermeasures
No main power connection Check power supply cable & Plug firmly.
No display NONE
Power failure Check main circuit breaker & Turn circuit breaker on.
NONE Low electrical power supply Check the main voltage.
Circuit breaker trip
Electric Leakage or shortage of heater apply for a service
Low Over Temperature Protection Setting apply for a service
Heating failure NONE
Heater disconnection apply for a service
Excessive steam Malfunction of the solenoid valve apply for a service

B. Error Messages & Countermeasures

PROCESS Display Cause Countermeasures
door The door is not closed certainly. Close the Door certainly.
WHOLE Er02 The heater thermostat open apply for a service
Er04 Fault of the RTD sensor Check the connection to the RTD.
Er10 Lack of the water Refill the water
FILL Check the SV1 – perform the Maintenance
Er1 1 Fault of the SV1(filling time over)
Check the CLS – perform the Maintenance
Er20 Over temperature in the chamber apply for a service
HEAT Er21 The heater aging(heating time over) apply for a service
Er22 The temperature is not rising. apply for a service
Er30 Over temperature in the chamber apply for a service
STER
Er31 Temperature Control Failure(±1.0℃) apply for a service
Check the SV1 – perform the Maintenance
Er40 Drainage Failure
VENT Check the Drainage part.
Er41 Exhaust Failure apply for a service
DRY Er50 The temperature is not falling. Check the SV1 – perform Self-test
* All the above mentioned parameters are changeable for the purpose of improvement without prior notice.
* Do not open the chamber door at any time until the pressure gauge indicating ‘0’.
* When you want to stop the process in case of the error occurrence and/or the malfunction, press the stop button
And then, the equipment can be stopped automatically after the Ventilation is finished.
* In case of the above cycle, water in the chamber may not be exhausted completely(It is not a malfunction).
* If you couldn’t fix the equipment after doing all above mentioned countermeasures, please apply for a service to a
technical expert.
* During apply for a service to a technical expert, please let him know of the Error Code & Serial Number.

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9. Maintenance
9.1 Cleaning of the chamber

① Take the tray rack out from the chamber.

② Wipe the dust off the inside of chamber using by the soft cloth.

③ Remove the dust on the heater using by a soft brush.

④ Whenever the cleaning the door, please carefully handle the door gasket on the door.

⑤ After finish the cleaning, fix the tray rack on the chamber.

9.2 Maintenance

① Remove the water completely from the reservoir tank while unused condition of unit
For a long time.

② Turn off the breaker switch on the bottom of back side and then keep the main plug

After turn it off.

③ Keep this unit in a dry place.

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 APPENDIX Ⅰ
P&I SCHEMATIC

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APPENDIX Ⅱ
MAIN CIRCUIT

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 APPENDIX Ⅲ
Environmental Health and Safety Internal
Weekly Inspection Log
Check for the following ;
◈ Chemical indicators supply ◈ Biological indicators supply

◈ Make sure the heater cover at the bottom of the chamber or water in the chamber clean

◈ Leakage of water or steam in pipeline

Date Name and Signature of Inspector Comments

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 APPENDIX Ⅳ
Autoclave Use List
CHEMICAL
NUMBER OF PRINT YOUR
PHONE BIOWASTE INDICATOR
DATE TIME CYCLE BAGS NAME AND
EXT (YES/NO) (If biowaste accept
(If Biowaste) LABORATORY
or rejected)

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