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Fastidious Gram-Negative Bacilli

This document discusses several fastidious gram-negative bacilli including Haemophilus, the HACEK group, Brucella species, Bordetella, Francisella tularensis, Pasteurella, and Legionella. It provides key details about their morphology, growth characteristics, and biochemical profiles to aid in identification. These organisms are challenging to isolate due to their fastidious growth requirements but understanding their phenotypes is important for clinical bacteriology.

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Nadene Kindred
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297 views18 pages

Fastidious Gram-Negative Bacilli

This document discusses several fastidious gram-negative bacilli including Haemophilus, the HACEK group, Brucella species, Bordetella, Francisella tularensis, Pasteurella, and Legionella. It provides key details about their morphology, growth characteristics, and biochemical profiles to aid in identification. These organisms are challenging to isolate due to their fastidious growth requirements but understanding their phenotypes is important for clinical bacteriology.

Uploaded by

Nadene Kindred
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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LICEO DE CAGAYAN UNIVERSITY

COLLEGE OF MEDICAL LABORATORY SCIENCE


CLINICAL BACTERIOLOGY LABORATORY

FASTIDIOUS GRAM-
NEGATIVE BACILLI

PRESENTED BY:
JOHN PATRICK B. TUDAS, RMT
HAEMOPHILUS
• FASTIDOUS, NON-MOTILE, CAPNOPHILIC AND
FACULTATIVELY ANAEROBIC BACTERIA.
• THEY CANNOT GROW ON PURE BLOOD AGARS
• BOTH CATALASE AND OXIDASE POSITIVE
(EXCEPT H.SEGNIS)
• GROWTH FACTORS: X (HEMIN) AND V
(NICOTINAMIDE ADENINE DINUCLEOTIDE OR
NAD) FACTORS, PORPHYRIN
HAEMOPHILUS
• GRAM-NEGATIVE,
SMALL, PLEOMORPHIC
COCCOBACILLI OR
RODS.
HACEK GROUP
1. HAEMOPHILUS SPP.
2. AGGREGATIBACTER
ACTINOMYCETEMCOMITANS
3. CARDIOBACTERIUM HOMINIS
4. EIKENELLA CORRODENS
5. KINGELLA SPP.
HACEK GROUP
• SMALL, NON-MOTILE, GRAM-NEGATIVE BACILLI
THAT CANNOT GROW ON MAC
• FASTIDIOUS (7-14 DAYS OF INCUBATION)
• ORGANISM UTILIZES AMINOLEVULINIC ACID
BRUCELLA SPP.
• OBLIGATE AEROBES AND INTRACELLULAR
PARASITES.
• NON-MOTILE, ASSACHAROLYTIC AND
NONCAPSULATED
• INCREASED CO2 FOR GROWTH (BRUCELLA
ABORTUS)
BRUCELLA SPP.
• SMALL
COCCOBACILLI THAT
ARE ARRANGED
SINGLY, IN PAIRS, OR
IN SHORT CHAINS,
AND HAVE A “SANDY
APPEARANCE”.
BRUCELLA SPP.
• COLONIES: SMALL, CONVEX, TRANSLUCENT,
YELLOWISH AND NON-HEMOLYTIC.
• CATALASE (+) AND OXIDASE (+)
• “RAPID UREASE PRODUCERS”
BORDETELLA
• OBLIGATE AEROBES, FASTIDIOUS GRAM-
NEGATIVE COCCOBACILLI.
• NON-CARBOHYDRATE FERMENTERS AND ARE
NON-MOTILE (EXCEPT B. BRONCHISEPTICA)
• BORDET-GENGOU AGAR
• CATALASE (+) , INDOLE (-)
• GROWTH FACTORS: NICOTINIC ACID, CYSTEINE
AND METHIONINE
FRANCISCELLA TULARENSIS
• SMALL, OBLIGATE AEROBIC, NON-MOTILE
COCCOBACILLI
• GROWTH FACTORS: CYSTEINE AND THIOSULFATE
• WEAK-POSITIVE CATALASE
• NEGATIVE OXIDASE
• ACRIDINE ORANGE STAIN
PASTEURELLA
• FACULTATIVE ANAEROBIC AND NON-MOTILE.
• GROWS ON SBA AND CHOC BUT MOST SPECIES
CANNOT GROW ON MAC.
• “SAFETY PINS” IN MICROSCOPY
• ALL POSITIVE FOR OXIDASE, CATALASE AND
INDOLE
• WEAK GLUCOSE FERMENTERS
LEGIONELLA
• FASTIDIOUS, AEROBIC, MOTILE AND NON-
CARBOHYDRATE FERMENTERS
• FAINTLY STAINING ON MICROSCOPY
• “RAINBOW COLOR” COLONIES IN BCYE
• INDIRECT FLUORESCENT ANTIBODY TEST (IFA)
LICEO DE CAGAYAN UNIVERSITY
COLLEGE OF MEDICAL LABORATORY SCIENCE
CLINICAL BACTERIOLOGY LABORATORY

END OF
DISCUSSION

PRESENTED BY:
JOHN PATRICK B. TUDAS, RMT

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