Flow EZ MAN - LU - 005-User-Manual-LineUp
Flow EZ MAN - LU - 005-User-Manual-LineUp
MANUAL
LINEUPTM SERIES
QUICK START GUIDE
Push-Pull
Ariaquick
The start guidesystem
is a perfusion which
is a forward automates
to the LineUpTMperfusion ormanual
Series user's timed
injection protocols. It allows for the sequential delivery of up to 10
different solutions at the desired flow rate into a microfluidic chip,
perfusion chamber or petri dish.
QUICK START GUIDE
2 3
4
5
2. This regulated (outlet) pressure pressurizes the reservoir,
driving the liquid up through the tubing and into the
microfluidic setup.
3. Controlling the outlet pressure allows one to control the rate
at which fluid is injected into the microfluidic setup.
3
QUICK START GUIDE
What is needed
Contained in the package
4
QUICK START GUIDE
5
QUICK START GUIDE
Power on
To wall socket
Once powered on, the Flow EZTM or
Push-Pull will display the "Inlet
pressure check window", giving the
measured pressure at the inlet
(P inlet) and the supply pressure
required to operate the pressure
controller (P target).
6
QUICK START GUIDE
Always wear safety protection
when manipulating pressure.
Pressure on
Pressure supply
7000 7100
-69
Different Flow EZ ranges
TM
-800
require different supply -345
pressures (shown to the right). -800
Note : If one intends to use compressed gas other than air, or if the pressure
supply is out of the pressure range requirements, please contact Fluigent.
7
QUICK START GUIDE
Push-Pull specificities
The Push-Pull module works the same way as other Flow EZTM modules,
except that it can output pressure (above atmospheric pressure)
and vacuum (below atmospheric pressure) through the same port.
1000 1100
-800 -800
To do so, it needs two inlets: the positive one, uses the standard pressure
supply of the Flow EZTM, and the negative one, utilizes an additional
connector located at the front between the FLOW UNIT port and the
"unlock" handle.
Special attention
should be taken to
Vacuum prevent liquid from
supply inlet flowing back into
the module.
8
QUICK START GUIDE
Note: The pressure supply is mandatory for the use of the Push-Pull even
in vacuum mode only. The vacuum supply is optionnal.
Please, never stack a Push-Pull module with a negative Flow EZTM as it
would not work.
9
QUICK START GUIDE
When connecting the pressure supply, if its value (P inlet) is not set at
the required value (P target), the Flow EZTM or Push-Pull will continue to
display the "Inlet pressure check window" (with the exception of the 7000
mbar range and the negative ranges which don't have this window)
When the two values are close enough, the P inlet value will turn green
and the display on the Flow EZTM or Push-Pull will transition to "Operation
window"
10
QUICK START GUIDE
Operation window
Press again to
Pmeas
recover pressure
Measurement of
Hold to enter sleep
the pressure in the
mode
reservoirs
If the system isn't calibrated, the outlet measured pressure (Pmeas) may
be incorrect.
11
QUICK START GUIDE
1 An example of 2
Pmeas before
calibration
10
3 4
Calibrating ...
1. Press "Menu"
12
QUICK START GUIDE
Aria is a connect
One can perfusion systemUNIT
a FLOW which automates
(Fluigent perfusion
flow sensor) or timed
between the
injection protocols.
reservoir and It allows for
the microfluidic the sequential
setup. delivery
(See page 22 of up
for more to 10
details)
different solutions at the desired flow rate into a microfluidic chip,
perfusion chamber or petri dish.
13
QUICK START GUIDE
When one has finished using the Flow EZTM or Push-Pull, hold
the "P=0" button in order to put the device into sleep mode.
If one needs to disconnect the power supply (e.g. to move or store the
unit), please make sure the Flow EZTM or Push-Pull is already in sleep
mode before disconnecting the power.
For a fully detailed shutdown procedure, please see page 32.
14
The Flow EZTM or Push-Pull is ready to be used,
Allowing one to get the most out of the LineUpTM series devices.
15
LINEUPTM SERIES
USER’S MANUAL
PRODUCT OVERVIEW18
SETTING UP21
A faster way to change the pressure21
Add a FLOW UNIT22
Flow rate control24
Inject a volume25
Flow-related setting 26
Liquid type27
SYSTEM EXPANSION28
Create a multi-channel configuration28
Mixing different pressure range29
LineUpTM Adapt30
COMPUTER CONNECTION31
LineUpTM Link31
LineUpTM Link COM32
SHUTDOWN PROCEDURE36
ADDITIONAL FEATURES37
FREQUENTLY ASQUED QUESTIONS (FAQ)38
WARRANTY TERMS40
TECHNICAL SUPPORT41
PRODUCT OVERVIEW
LineUp Flow EZTM
Pressure controller
2 4
18
PRODUCT OVERVIEW
LineUp Push-Pull
Pressure controller
2 4
19
PRODUCT OVERVIEW
1 2
3 4
5 6
1
1. Press the dial to
enter "command
off" mode (the
Pcmd value blinks).
3. When one
3 wants to apply the
pressure order,
press the dial again.
21
SETTING UP
The FLOW UNIT is Fluigent's flow rate sensor. When a FLOW UNIT is
added, one can use the Flow EZTM or Push-Pull to:
• Monitor the flow rate in the microfluidic set-up
• Directly control the flow rate locally
• Perform an injection, based on either volume or time
Connect the FLOW UNIT inline with the arrow indicating the
direction of the flow to get a positive value. This is important for
the flow rate control to work properly. Please pay extra attention
when using a Flow EZTM of negative range, or when using a vaccum
with a Push-Pull, in which case the values will be negative.
22
SETTING UP
1
Qmeas H20
The measured flow rate
87.00
2 M µL/min
(Qmeas) is only monitoring
Pmeas 4
432.0mbar purposes. To directly control
Pcmd the flow rate, see next page
432.0mbar 5 (Flow rate control)
3 Set Q Ctrl Menu
1 Qmeas H20
M
87.00
µL/min 1
Measured flow rate
Units displayed can be chosen
Pmeas
432.0mbar 2
Flow rate command
To be set by the user
Qcmd
Go back to pressure control
2 87.00 3
mode
µL/min
Set P Ctrl Menu
The user can directly control the flow rate, by setting the flow rate
command (Qcmd)
Although the control mode is in flow rate, the live pressure section
value in the reservoir (Pmeas) is still displayed in the middle,
giving information on the fluidic set-up. Abnormal flow rates may
reflect problems in the microfluidic set-up (leakage, clogging, etc.)
Direct flow rate control works only when the value of measured flow rate
(Qmeas) is positive. If it is not the case, please verify that the FLOW UNIT
is connected in the direction with the arrow indicating the fluid path.
24
SETTING UP
Inject a volume
When a FLOW UNIT is connected, one can inject a certain volume
into the microfluidic set-up. To do so, select "Injection method" in
the menu.
1. Turn the dial to set the "Injection flow rate" to the desired
injection speed.
Injection flow-rate
1 The "Injection method" described is
5.00 µL/min
only available when the Flow EZTM or
Target Target
volume time Push-Pull is in stand-alone mode. When
connected to a PC, the injection operations
2
need to be initiated by Fluigent software.
Back OK
25
SETTING UP
Response tim e
(nominal use case)
The response time is valid for
Turn the dial to set a longer response time (between 2 seconds and
1000 seconds)
Liquid type
The type of liquid is set manually using the menu. Selecting the corresponding
liquid type makes the flow rate measurements more accurate:
Note:
In the case of range M FLOW UNIT, additional liquid types are possible:
• FC40
27
SYSTEM EXPANSION
Creating a multi-channel configuration
The Flow EZ TM and Push-Pull have a hot plug & play feature:
28
SYSTEM EXPANSION
However, if a Push-Pull 69
150
is integrated, it needs to 25
h ave a vacuum supply
-25
in order to perform
-69
negative pressure orders.
-800
-345
A Push-Pull cannot be stacked
-800
in with a negative FlowEZTM
29
SYSTEM EXPANSION
LineUpTM Adapt
If the ranges have different required supply pressures, one can
still have them in the same chain by inserting an Adapt module
between them.
1 2 3 4
30
COMPUTER CONNECTION
LineUpTM Link
The LineUpTM Link allows to connect the
LineUpTM series modules to a computer.
Th e L i n k a l l o w s t h e p r e s s u r e c o n t r o l l e r t o b e n e f i t f u l l
a d va n ce d f u n c t i o n a l i t i e s f r o m Fluigent's sof tware s u i t e .
31
COMPUTER CONNECTION
Stop Bits 1
Parity No parity
32
COMPUTER CONNECTION
For all commands (no question mark “?”), the data column represents the
required parameters to be sent to the instrument following the string in
the command column. Any command that requires multiple parameters
must have the parameters separated by commas “,”. In case of error in
the commands spelling, the command is ignored by the instrument
without error code returned.
33
COMPUTER CONNECTION
SYST
Change baudrate
:SETBR:<value> 0=9600, 1=19200, 2=38400, 3=57600,
4=115200
34
COMPUTER CONNECTION
CHAN
1 Press and hold (for a few seconds) the "P=0" button in order to:
Use the "Chain to Chain Kit" (sold separately) to give more
flexibility when placing the multichannel configuration on the lab
bench.
37
FREQUENTLY
ASKED QUESTIONS
The Flow EZTM or Push-Pull cannot achieve the requested
pressure or flow rate, what should I do?
38
FREQUENTLY ASKED QUESTIONS
Some older FLOW UNITs are not supported by the Flow EZTM or Push-
Pull. In this case, please contact Fluigent at [email protected]
Can I use the LineUpTM Push-Pull even if one of the two inlets
is not supplied, as a regular positive or negative Flow EZTM?
39
WARRANTY
TERMS
What this warranty covers
This warranty is granted by Fluigent and applies in all countries. The Fluigent product is guaranteed for one
year from the date of delivery at the laboratory against defects in materials and workmanship. If found to
be defective within the warranty period, the Fluigent product will be repaired or replaced free of charge.
This warranty does not cover routine maintenance, or damage resulting from the failure to maintain the
product in accordance with instructions provided by Fluigent. This warranty also does not cover damage
that arises from accidental or intentional misuse or abuse, alteration or customization, or repairs by
unauthorized persons.
If there is a problem, please contact the Fluigent sales office from where one purchased the product(s).
Arrange a mutually convenient time for Fluigent service representative to discuss and find a solution to
fix the issue. Repairs will be made remotely whenever possible. If more action is needed, the system will
need to be sent back to Fluigent offices (for no additional cost, only if it is under warranty).
Warranty conditions
Do not open any LineUpTM series device (opened devices will not be charged by the customer support)
Do not use cables and power supplies other than the one provided by Fluigent
For negative ranges of pressure, please do not apply any positive pressure
Prevent any corrosive liquid from coming in contact with the device
40 For questions about specific uses, please contact Fluigent support team at [email protected]
TECHNICAL
SUPPORT
Still have questions? E-mail us at:
For a fully detailed FAQ for all Fluigent products, please visit:
http://www.fluigent.com/faqs/
http://www.fluigent.com
For tutorial videos about the LineUpTM series, please visit Fluigent on YouTube
Fluigent
41
42
43
VERSION
JAN. 2021