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Microbiology and Parasitology: Exercise No. 5

This document summarizes a microbiology student's laboratory exercise on bacterial culture media. The objectives were to understand the importance of bacterial culture media and demonstrate knowledge of preparation. Various bacterial culture media were identified and their uses discussed, including obtaining pure cultures for studying microbes. Quality media is necessary for accurate microbiology tests. Bacteria play important positive roles like producing antibiotics and supporting life. Growing bacteria in controlled pure cultures remains a key technique and different media contain nutrients to support bacterial growth and reproduction.

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Joshua James Mendez
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64 views4 pages

Microbiology and Parasitology: Exercise No. 5

This document summarizes a microbiology student's laboratory exercise on bacterial culture media. The objectives were to understand the importance of bacterial culture media and demonstrate knowledge of preparation. Various bacterial culture media were identified and their uses discussed, including obtaining pure cultures for studying microbes. Quality media is necessary for accurate microbiology tests. Bacteria play important positive roles like producing antibiotics and supporting life. Growing bacteria in controlled pure cultures remains a key technique and different media contain nutrients to support bacterial growth and reproduction.

Uploaded by

Joshua James Mendez
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Name: Joshua James Mendez 

            Date Performed:  April 22, 2021

Year and Course: 1st Year BSN   Date Submitted:  ____________

Subject title: Microbiology and Parasitology Code number: 9231

Name of Professor: Angeli Emborgo Score: ______________________

Exercise no. 5

                                          Bacterial Culture Media

INTRODUCTION

 Bacterial culture is essential for the study of its virulence, its antibiotic susceptibility, and its genome
sequence in order to facilitate the understanding and treatment of caused diseases.

OBJECTIVES:

At the end of the experiment, each student will be able to:

 a) know and understand the importance of Bacterial Culture Media 

b) Demonstrate knowledge on the bacterial culture media  preparation in    

    Microbiology. 

MATERIALS

              Laboratory Manual

              Microbiology Notes

 PROCEDURE

Identification and/or visual preparation of  Bacterial Culture Media 

 I. Bacterial Culture 

    a) Identification of the bacterial culture media in 

       Microbiology.

    b) Provide a picture/video of a Bacterial Culture Media preparation.

II. Importance and Uses of Bacterial Culture Media.

    a) A review on the different uses of bacterial culture media preparation 


        commonly used in microbiology. 

III. Post Laboratory Analysis

     a) Answer the questions related to the laboratory activity.

IV. ANALYSIS 

     a) Why is it necessary to know the importance and uses of the Bacterial 

           Culture in Microbiology?

To obtain pure cultures, grow and count microbial cells, and cultivate and select microorganisms, most
microbiological tests require culture media. Without high-quality media, the chances of obtaining
accurate, reproducible, and repeatable microbiological test results are reduced. 

V. CULTURE MEDIA PREPARATION

      

     Culture medium must contain adequate nutrients to support bacterial growth and this would include
organic compounds that can provide the building blocks necessary for cellular reproduction. 

   Since bacteria is a known cause of human disease, bacteria really should be far more famous for their
positive contributions than for their negative ones. 

     a) Below, list tat least three positive things that bacteria do for you

1. Bacteria can be used for making drugs like antibiotics and also vaccines

2. Bacteria are essential to human beings in terms for supporting life.

3. It help us prevent the invasion of diseases-causing bacteria.

  b) Identification of the Bacterial  Culture Media Preparation .

       (Please refer to the notes given to you ).

 
COLONY COLONY PICTURE COLONY PICTURE COLONY PICTURE
SIZE MARGIN FORMS ELEVATIO
   
N
Punctifor Entire Circular Flat
m

Small Undulate Rhizoid Raised

Medium Lobate Irregular Convex

Large Filamentou Filamentou Pulvinate


s s

Large Curled Umbonate

  

       
VI. CONCLUSION

Growing bacteria in pure culture is still one of the most popular microbiology techniques. Many
bacteria, particularly those that cause diseases and those that are used in scientific studies, are
heterotrophic, meaning they depend on organic compounds for food, energy, and carbon. A community
is a group of bacteria that has been developed in a laboratory. A pure culture contains only one type of
bacteria, while a mixed culture contains two or more types. If a bacterial culture is held in the same
media for too long, the cells deplete the nutrients available, excrete toxic metabolites, and eventually
die.

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