LIST OF BIOLOGICALS 4, HEMORRHAGIC SEPTICAEMIA VACCINE (Alum Precipitated 2. HAGMORRHAGIC SEPTICAEMIA OIL ADJUVANT VACCINE 3, BLACK QUARTER DISEASE VACCINE 4. LISTERIOSIS VACCINE ‘5. CONT: CAPRINE PLEUROPNEUMONIA VACCINE (Freeze Dried). ‘6. ENTEROTOXAEMIA-CUM-LAMB DYSENTRY VACCINE 7. ANTHRAX SPORE VACCINE 8. SPIROCHAETOSIS VACCINE 8, RINDERPEST CELL CULTURE VACCINE (TCRV) 410. FOOT AND MOUTH DISEASE VACCINE (Cell Culture) ‘14, ANTI RABIES VACCINE (Semple’s) 12. ANTLRABIES VACCINE (Flury) LEP SINGLE SHOT 43. ANTI-RABIES VACCINE (Fulry HEP) SNGLE SHOT. 44, SHEEP POX CELL CULTURE VACCINE 415, GOAT POX CELL CULTURE VACCINE. 16. NEWCASTLE DISEASE VACCINE ‘17, FOWL POX VACCINE. 48, HYDROPERICARDIUM SYNDROME VACCINE 19, FOOT-AND-MOUTH DISEASE SERUM 20. MALLEIN (Ordinary and Concentrated) 21, TUBERCULIN P.P.D. (Avian and mammalian) 22, BRUCELLA ABORTUS AGGLUTINATION CONCENTRATE, 23, BRUCELLA ABORTUS MILK RING TEST ANTIGEN. 24, ROSE BENGAL PLATE TEST ANTIGEN. 25, SALMONELLA PULLORUM STAINED ANTIGEN DIRECTIONS FOR USE OF BIOLOGICALS Produced by VETERINARY RESEARCH INSTITUTE Zarrar Shaheed Road (ghazi Road) Lahore, Cantt -13. Telephones Director: ‘Supply Section oxzotat PABX Excharge No, s220136-8 2 Fax 9220142 Telegram: VetresBIOLOGICAL PROUDC: INTRODUCTION ‘The Veterinary Resesarch Insutive, Lahore Cant offers ‘means for proving the necessary neath cover for estock and Poully industry though manufacture of immune sera, vaccines = and diagnostic agents. The proudets are properly tested for ther — sterility. safely and potency and confoim to Intemational standards Vaccines: - Vaccines are preparations of antigenic material and are adminsrered wih the objective of inducing oF increasing an acive mnmulty in. the recipients. They may. contain Ive, Stienusted or dead bacteria or virus or ther by-product. ‘Vaccines produced at he Veterinary Research Insitute (VI) ate of two types: (A) Bacterat ©) Viet (A) Bacterial Vaccines. ‘The folowing Decteral vaccines, which are in common use, are being manufactured at Present (6) Fortwestock Hacmerragle Septcaemia Vacone(awant & oi 358 Black Quarter Disease Veccine Listerosis Vaccine Contagious Caprine Pleauropneumonia Vactine EEnterotoxsemie-cum-iamb Dyesenty Veccmne ‘Anthrax Spore Vaccine (©) For Poutey ‘ (0) Spirochaetesis Vaccine(8) Viral Vaccines (o) For Livestock (). Rinderpest Cet cuture Vaccine (TERY) (i) Foot-and:Mouth Disease Vaccine (Call Cuture) (ii) Ant-Rabies Vaccine (Semple's) (i) Ant-Rabies Vaccine (Fury) HEP & LEP (\) Sheep Pox col Cuture Vaccine (0) Goat Pox cell Culture Vaccing (@) For Pouty ()__ Neweaste Disease Vaccine (@) Fowl! Pox Vaccine (i) Hydropericardium Syadrome Vaccine Antisera Antisera. are native (unconcentates) sera, or preparation ftom native sera_containing antibodies obtained from hyperimmunised buffalo calves Thay af used for offering immediate passive enmuniy for ransiont pefied: With the advent of modein antibicctic therapy. the use of ‘most of the antisera has been cliscontved, However, Foot and: Mouth Disease Serum is sil in use ands Bang prouduced, Diagnostic Agents ‘The phenomenon of allergy in-vtvo and serological reactions inwiio (Specific antibody anigen reactions) are exploted to Giagnse some diseases in the clinical or subclinical stage through such agents ‘The folowing diagnostic agents are being produced () Matein (i) Tabereulin PPO (Avian & Memmatan) (it) Brucella sborus agalutnation antigen (conconirato) (iv Buroalie abortus . Mik Ring Test Antigen |W) Rose Bengal pate test Anticen {) Saimoneia pullorum Staines Antigen INSTRUCTIONS FOR USE AND DOSAGE General + The vaccines are intended for prophylactic and not fer curative purpose + The bottle should be thoroughly shaken before each injection to ensure a unform suspension, ‘+The sysinges, needes and other instruments used should be ately steriised before use + The botles, ampoules, vels oto. must not be exposed to cirect sunlight or heat, Shelesfe depends on prope” storage. ‘+ Protective immunity evel can be aehleved and maintained through sdopting a planned snd systematic shedule of vaccination + Incase of any back reference, batch number and date of ‘manufacturer of the product, 28 mentoned on the label should be quoted + Live virus vaccines. should ct be demanded through cttinary post tis preferable to colect nem in ie fom the Insitute through a special courier + Detiled water for reconstitution of vaccinae must be stotized & chiles + Alter reconstition the incoulum must be Kept cool and ‘eee within 2 hours. ‘+ Empty ampoulesiials and ten-over inoculum should be propely disposed off 41. Haemorrhagie septicaenia vaccine (alum Precipitated) Itis ¢ formalized alum precipitated bacterin of selected local “tran of pasteurela multecisa Rober type-1. The vaccine Ie used ‘wes a years, preferably im Mayilune and Novermber/December Packing’ Bole containing 200m! each (60 doses) ‘Shetife: Six months i stored ina coo, ery datk place Dosage: Smi300kg body weigh to be given subcutaneously. Booster Dose:- Booster Cose is necessary ina Calves ater 4 weeks of fst vaccinationforthagic Septicaemia Oil Adjuvant Vaccine, Itis formalized kills il adjuvant vaccine prepared fiom local sirein of Pasteurelln multocida, Robert Type, The vaccine is ‘ceed once a yea Packing: Booties containing 300 ml each (60 doses) ‘Sheleife: Sx months i stored under retsgerator Dosage: Sl per animal to be given deep intramuscular, Booster Dose, Booster dose in calves in necessary after 12 ‘neck of fest vaccination Proce Re. 180 3. Black Quarter Disease Vaccine. sis on slum-preciptates formalised anacuitre ofc. chauvoo! Injection confers immunty for 6 months Vaccination i ser 15 days would confer solid immunity for one year. ‘5ml600 Ibs body weight Bottles containing 300 mi each (60-doses) ‘ron Fsloved in ¢ cool ry an dark aes. BQ. Vacoine accesionally gives allecic at the time of vaccination antvallemie drug should be kept ready as a precautionary messes Usteriosis Vaccine is 2 formalised bacterin of 2 local stain of Litera “Rerccytogenes, Vaccine is used twice @ year i March and ‘September. tis prepsred againts spectic demand. $Beceng: Bots containing 300m “Sbebie: 6 months stored ina coo, cry anc dank pace. ‘Besage: 57/600 Ibs body weight, subcutaneously Cont: Ceprine Pleuropneumonia Vaccine {tis a formalised cure of Mycoplasma myeoides var capri in 120% serum mycoplasma, broth Packing: 100 dose vals ‘Shel-ife: One year kept at °C Dosage. ‘mi subcutaneously 4 Reconstitution: The contents of the vial are suspended in 700m) sterile disiled water and thoroughly mixed before use. 66, Enterotoxaemia-cum-lamb dysentry vaccine It ie an alum preciptated formalised whole culture bactn preparad by incorporating equal quantiles of Perfinegens tyoes B& 0 Culture. Immunity taste for about 6 months. Vaccination i repeated aftr 410 6 waeks would confers sols immunity for o5€ years Packing: Bottes containing 300 ml ‘Shelf: 6 months if sored in a cool dry and dark place. ‘Dosage: Adult sheep 3m Lambs 1-2 mi lambs under 3 months ‘should nat be vaccinated. Ewe should be vaccinated 7 to 10 days before paruntan confers immunity in newborn lambs for 210 3 months 7. Anthrax Spore Vaccine Its a euspension of ive alenuated spores of non-cepsulated Bacilue anthracis in alycorne saline. it confers soli immunity for ys, Packing: Bote containing 200m ‘Shet-ife: One year if sored in cool. dry and dark place. Dosage: Cattle, Sufaloes and Horses 1mi subicut, Sheep and goals 0 5m 8. Spirochaetosis Vaccine ts a formaises whole blood cuture of a local strain of Borrelia anserna ‘Vaccine is used twice 2 year preferably in March and September Packing: Each ampoule conta 100 doses. ‘Shai: 6 months i kept at °C Dosage: imi subcutaneously. Reconsitution: Acd the contents 10 few mi of stenle distiled water’ and titurate in sterile peste and mortar ‘Then add move cistiled water to make 100 mi.9. Rinderpest Cell Culture Vaceine (TCRV) itis 8 ve attenusted strain of RBOK anderpest vis grown on cell cuture. Vaccine is safe for excte stock as well as Progrant animals. It shows no untoward. teaetion and produces soll immunity 2-3 weeks ater vaccination Packing: Vial of 100 doses. ‘Shelife:6 months if sored at °C , 24 months at-20°C Dosage: imi subcutaneously ‘Reconsttution. Reconsttute in 100ml stenleenlled NSS, The ate Vaccine is not supplied through ordinary mai collec it through special Goumrer who shouts bring thermosflask wth fo 410. Foot and Mouth Disease Vaccine (Cell Culture) contains Asia * & 0 types of FMD virus, grown on BHK cel line. The vaccine is cafe for alleges, Packing: 300m per bot ‘Sheteife:6 months a °C (Do not freeze) Dosage: Cate & Bufaloos Sel = Sheep & goat 2 5mi, subcutaneously Schesule of Vaccination: {stinoction... At6 months of age 2nd injection At 10 months. ‘Therestir one Injection every six months ‘Note: The vaccine is not supplied by ordinary post. Collect through special courier, who must feng an ie-box containing ies ‘1. Anti Rabios Vaccine (Semple’s) Itis phenclsed 6% suspension of sheep brain infected with Pasteur stain of Rabies fed Vitus. Packing: Bottes containing. 448m! 260m, 7Oml Shelhife: 6 montne a 4"6 6 Species ‘Guanily Daily No.of ofvaccine Dose Inaculat inm_inmi_ ions Cartel wo 60a Horse Mares Cattle Buffalo 430 a Mules and Donkeys 2 Calves 70 Fiipenasta ‘Sheep and Goats 70 eens Dogs above 30 bs a 10 Dogs under 30 Ios 35 sre Cats 35 sce Zoo animale. ‘According to “body weight 42. Anti-Rabies vaccine (Flury-LEP) Single Shot Itis a chick embryo adapted lve virus vaccine empoyed for prophylactic enmurizaton of dogs, Packing. Single dose sil Shofsife: 6 months f sored at °C and 2 years at 20°C Dosage: Full dose for adult, 1/2 for pups (2 to 6 months ok) siven intramuscular. Reconsttuton: Dissolve in Sm sterle chile cistiled water. 48. Anti Rabies Vaccine (FLURY-HEP) Single Shot. It is a chick embryo adapted lve vaccine employed for prophylactic immunization of Cate and leige anenals, Packing: Single dose Vial ‘Shelctte: 6 months if stored 4°C and two years 31-20% Reconstitution: Dissolve in 3 dstiled water or physiological saline 14 Sheep Pox Cell Culture Vaccine It is an attenuated lve views cell cuture vaccine. (RMLES suai) Packing: Vial of 100 doses. ‘Shettife 2 years at- 20°C, Dosage: ml. subcutaneously, Reconstitution: Reconstituie in 100m. of chiled distiled \wateripnysiological saline and use within 2 ous. y18, Goat Pox Cell Culture Vaccine, Its 2 ve atenuated stain of ‘grown on primary Kid kidney cel is safe for ‘katie flock 3s well as pregnant ‘no untoward feaction and proudees sabd mmmuniy vaccination for one year, Packing. vials o 100 doses, ‘Shoife: 28 months at- 20° Dosage: Imi, subcutaneously Reconsttution’ The contents of Wi 100ml ster a econstitvied in INS. Solution Note ‘The vaccine is na rough ordinary mal, Collect i courtier who should bring ee 16, Newcastle Disease Vaccine Iris 2 lve vius vacemne prepared bryo adapted local strain of virus Packing: 100 dose vials /500 dose vials. Stet: 2 year sed 20 ond 8 moms ato Bosoge, 1% doe ntacrorto Tos se 7 coccf ir ntornscaatanaimees 5 Seo rn nvamusel af foes Reconstr” ‘Recontte fe Commie ne amupul in 5.0 mi chiled distilled water for 2st dose and ino op 100 eed ited ta or 2" and 3" dose, 3 17. Fowi Pox Vaccine. Itis a chick embryo adapted live vitus wassine prepared fom a strain of reduced vinience. Packing: 100 doses vial Shefife: 2 years st -20% and 6 months ate Reconsttution: Dissolve the contents im 0m! coo! slerile ‘istiled water. Vaccinate by wing-web Puncture using @ 2-pronged needle, Use ‘ropared vaccine within 2 hours Note "Takes" must be examined 6-8 days after vaccination, 18, Hycropericarcium Syndrome Vacci Its a killed formalized Suspension of Adeno vius prepared from ver of fected chic Packing: 250 mi ott. ‘Shefelfe: 1-2 Months at &to 8% Dosage: 0.3m! to be inecied subct at the age of 10-13 days in broiler hicks. 419, Footand-Mouth Disease Serum Ik isa polyvalent serum obtained ftom buff calves hyperimmunised against various stains of FMD and is used for passive immunization against Footanc-Mouth Disease, Protection afforded is immediate but short ved, Packing: 300mi ett. ‘Sheife 6 months if sored ina coe, cry anc dark place. (o not freeze) Dosage: SOmi600 bs body weight 20, Malloin (ordinary and concentrated) Molin ordinary or concentrated, is an endotoxin, released by Pseudomonas malt in a sulabie flue meclum. Its used forthe diagnos of Ganders in Equine Packing: () Ordinary: Vial of Sml(S doses) ‘Dosage: (ji) Concentrated: Vial of Sl (25 doses) ‘Sholtife. 3 years stored at 4°c (D0 not freeze) Instruction for use LDP. Test: Inject 0.2ml concentrated malein into the dermis fof lower eyeld, 144 below tho eye lashes. Positive reaclon Becomes defined at 12 hours reaches ils maximum at 24 hours and persists upto 48-72 hours. The eye id becomes odematous, tender and hat; conjunctiva i injected, sulphur coloured exudate Aeposis on the edge of eyed and mucopuruient dscharge appears at inner canthus, ‘Sight oedema around the sito of incculstion and conlucivts ‘hat persists for 48.72 hours, i taken as doubtful, Repeat the test ion the other eye after an interval ofa week, ‘Sweilng that (8 netther tenger nor persistent ie t2¥e7 as negative reaction, aSubcutaneous Test Record the temperature of animal every 3 hours and if t remains normal for 12 hours inject ml ordinary mmallsin subcutanecusly at a clean and dsinfecied sie in tho mile ofthe neck. itis Convenient to inject mali at 800 p m and start recording temperature next morning st eur inlewal Starting from 6 00 am, upto 9.00 pm Poste reaction is manifested by a ise of 25°F er more ucto 24th hour which subsides slowly. Senultaneously a ees! reaction Appears upto 36 hour, at the ste of inoculation i ie for of inverted saucer staped., hot, tender, oedematous Swating of 6 Inches diameter of more. The sweling recedes slawiyin @ Berd of se days < Doubtful reactions are less matked, and the retested after 3 wooks. Very sight rise in te repicly dsapnearing sweling Is taken as negative 21. Tuberculin PP. (Avian and Mammalian) 1s @ puried protein dervatve obtaines Mycobacterium tuberculosis, Packing: Vial of 50 doses. ‘ShetPife: 36 months i stored in cool. dry and dare Dosage: 0 tml intradermally Instructions for use: ‘On one side of the neck, avian and mary injected ata dstence of § to Som The results aie hours ae 3 When therein no non specific reaction and ineiease ef 4mm ‘oF mare is regarded as postive. An increase 3 38 mm 2 oubdul, Ivespectve of measurement any cifluse sweling regarded as posiive 22, Brucella Abortus Agalutination Concentrate Itie@ kild phonalsad cuspension of Srucela abortus, stain 99, used fr serological tests fo the diagnosis of Brueslosis, Packing: SOm| totes. ‘Shette, 3 years if stored at °c (Do not feaze) Note Ik is. supplied to laboratories dealing in agnostic work where serological ests can be conduct, 40 23, Brucella Abortus Milk ring Test Antigen Itis a stained suspension of Brucella about stain 99, which s used for Abortus Bang Ring (ABR) Test for the diagnosis of Briceloss Packing: 25m\ bot ial ‘Sheff: 3 year stored at 4c (Do not freeze) Instructions for use: ‘Add | drop of antigen in 1 ml of whole flesh mik in @ tube ‘Shake well and keep inthe incubator at 37° for hour A deep blue coloured ring appearing on top is incicatwe of postive 24, Rose Bengal plato test Antgen: ‘The antgen consists of Brucella ces (St. 98) stainad wih se Bengal and suspended in buter at PH 26 and « used for the diagnosis of Brucellosis, Packing. Sm Vial SShotife: The antigen remains stable for long period when stored at 4°c, it deteriorates. when constantly removed ftom the ‘refrigerator and if standing on the table fr prolonged periods. Inotevetion fr Use: “The lest essentialy consists of mixing equal volume of antigen and serum (0.08mi) and observing far agglutination unt the and ofthe 4 minuie’s period 24, Salmonella Pullorum Stained Antigen Itis stained suspension of selmonelia pulorum (St. 11 & St. Veriant) used for rapid’ whole blood. diagnostic test of Salmonellosis in fowls, Packing: 25m bottle Shot: 3 year if stored at (Do not freeze) Dosage, im, subcutaneously, Instructions for use: | drop of antigen Is mixed with | drop of blood on @ side, Blood of postive bird agglutinates within 30 fo 60 seconds. Delay In agglutnaion or appear ance of re cut ike particles indicates Fetosting ater a week, "PRICE OF VACCINE/MEDICINES Taerarabie OI Aol Vcsne aemecai Sepeseria indent Vacs (CRY pane Fase Preumonia Vac ann Concent 2%) Mate ovina. rate AborueAnigen AR) rea Abrus Ange (Con) Bote of 0 WEDS] bot of 3c0n (9 Dose) ote of ton (D0 Doses) ow ot 00 ane] ote of 00 roses) Bot of 00 ase Bent of to 6 eee) Bot of (6 ome) Bont ot ir 10 aoe) Bot 10 anaena 12 mo ose Sune! Artgen. Mal ote See on ot C0 doses. ‘nt Rae Vaccine famole') Bott of at fr ert, - Bett of 35efor doit B‘Monty January Febeuary Maren Aor May June “uly August October November December s ‘TENTATIVE VACCINATION SCHEDULE Calla & Burfaloes FMD Ba Hs a) Rinderpest HS 14 Sheep Enterotoxaemia ‘Anthax ‘Sheep Pox FMD Pleuropeumonia Sheep Pox FMD Pieuropreumoni Enterotoxsemia. * Optional ‘Anthian Gost Pox EMO. Prouropneumenia Pleuropneumenia Enterotoxemia