National Pharmaceutical Control Bureau

MINISTRY OF HEALTH MALAYSIA

Analytical Method Validation

Common Problem 2

Centre for Quality Control

National Pharmaceutical Control Bureau
Lot 36, Jalan Universiti, 46200 Petaling Jaya, Selangor

DL: +6.03.78835400 | F: +6.03.79567075 |

WS : www.bpfk.gov.my |
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OUTLINE

1) Linearity & Common Problems

2) Accuracy & Common Problems
3) Precision & Common Problems
• Method precision
• Intermediate precision
 Type of analytical Identification Testing For Impurities Assay
procedure Quantitation Limit - dissolution
characteristics (measurement only)
- content/ potency
Accuracy - + - +

Precision
Repeatability - + - +

Interm. Precision - + (1) - + (1)

Specificity (2) + + + +

Detection Limit - - (3) + -

Quantitation Limit - + - -

Linearity - + - +

Range - + - +

- signifies that this characteristic is not normally evaluated

+ signifies that this characteristic is normally evaluated
(1) in cases where reproducibility has been performed, intermediate precision is not needed
(2) lack of specificity of one analytical procedure could be compensated by other supporting analytical
procedure(s)
(3) may be needed in some cases
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Linearity and Range

Linearity Range
 The linearity of an analytical  … the interval between the

procedure is its ability upper and lower

(within a given range) to concentration (amounts) of
obtain test results which are analyte in the sample
directly proportional to the (including these
concentration (amount) of concentrations) for which it
analyte in the sample has been demonstrated that
the analytical procedure has
a suitable level of precision,
accuracy and linearity

ICH Q2(R1) Guideline, 1996

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Linearity and Range

• Assay of a drug substance or a finished (drug)

product: normally from 80 to 120 percent of the
test concentration;
• Dissolution testing: +/-20 % over the specified
range;
• Determination of an impurity: from the reporting
level of an impurity to 120% of the specification;
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Linearity and Range - Assay

 Standard stock solution (S1)  Requirement: 80 -120%
methanol
of the test concentration
25mg X 100mL  80% ??
 Standard working solution = 0.01 mg/mL x 80%
methanol = 0.008 mg/mL
2mL S1 50mL  120% ??
 Calculate 100% conc., = 0.01 mg/mL x 120%
= 0.0012 mg/mL
25 2
x = 0.01 mg/mL
100 50
Range: 0.008 - 0.0012 mg/ml
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Linearity and Range – Impurity

• Requirement: LOQ to 120% of the specification

• For example, specification of Imp A = NMT 0.5%
• Working concentration = 1 mg/mL
• 100% = specification of Imp A, 0.5% (in this case)
• What is the concentration of 120% ??
• = 0.5% x (120/100)% x 1mg/mL
• = 0.6 mg/mL
• Range: LOQ - 0.6 mg/ml
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Linearity and Range – Assay + Impurity

 Standard stock solution (S1)  LOQ
methanol  120% ??
25mg X 100mL = 0.01 mg/mL x 120%
 Standard working solution = 0.0012 mg/mL
methanol
2mL S1 50mL Range: LOQ - 0.0012 mg/ml
 Calculate 100% conc.,

25 2
x = 0.01 mg/mL
100 50
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Linearity and Range – Dissolution

 NLT 75% of the LC dissolved in  Requirement: +/-20 %
30 minutes. over the specified range
 Standard stock solution (S1)
 75% - 20% = 55%
methanol = 0.01 mg/mL x 55%
25mg X 100mL
= 0.0055 mg/mL
 Standard working solution
 75% + 20% = 95%
methanol
= 0.01 mg/mL x 95%
2mL S1 50mL
= 0.0095 mg/mL
 Calculate 100% conc.,
25 2
x = 0.01 mg/mL Range: 0.0055 - 0.0095 mg/mL
100 50
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Linearity
Data Required Testing Method
 Testing Method  Minimum of 5 concentrations

 Acceptance criteria over a suitable range

 Data for linear regression  dilute stock solutions or

equation, Y-intercept, slope, separate weighings

r2 and linearity graph.
Acceptance criteria
 visual – straight line graph.
2
 r > 0.995

 Y - intercept at 100%
working concentration ≤ 2%
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Example of Linearity
Data and Curve
1) Common problem 1

• Testing method not

given – preparation
of each target
concentration ??
2) Common problem 2

• Y – intercept at
100% not given
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Example of Linearity Data

and Curve

How to calculate y-intercept

at 100%?

In this case,

= 0.8% (A.C. = NMT 2%)

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Common problem 3:
Do not provide data within the specified range
Linearity data for Cyanoguanidine
(an impurity of Metformin HCL)

Product with more than 1 strength ??

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Common problem 4:
Do not provide sufficient data (1)

 Insufficient linearity data

 minimum of 5 concentrations
over a suitable range
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Common problem 4:
Do not provide sufficient data (2A)

 No peak area value for each

point of linearity in tabulated
form
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Common problem 4:
Do not provide sufficient data (2B)

• No peak area value for each point of linearity in tabulated form

 Type of analytical Identification Testing For Impurities Assay
procedure Quantitation Limit - dissolution
characteristics (measurement only)
- content/ potency
Accuracy - + - +

Precision
Repeatability - + - +

Interm. Precision - + (1) - + (1)

Specificity (2) + + + +

Detection Limit - - (3) + -

Quantitation Limit - + - -

Linearity - + - +

Range - + - +

- signifies that this characteristic is not normally evaluated

+ signifies that this characteristic is normally evaluated
(1) in cases where reproducibility has been performed, intermediate precision is not needed
(2) lack of specificity of one analytical procedure could be compensated by other supporting analytical
procedure(s)
(3) may be needed in some cases
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Accuracy
The accuracy of an analytical procedure expresses the
closeness of agreement between the value which is
accepted either as a conventional true value or an
accepted reference value and the value found

“Trueness”

Accuracy should be established across the specified range of

the analytical procedure

ICH Q2(R1) Guideline, 1996

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Accuracy
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Accuracy
Data Required Acceptance criteria
 Testing Method  % recovery by the assay of

 Acceptance criteria known added amount of

analyte in the sample (95 –
 Raw data in tabulated form
105%)
 % recovery or mean OR
difference and confidence
interval should be reported  difference between the
mean and the accepted true
value (± 2%)
Testing Method
 Minimum three (3) levels of
concentration in triplicates
covering the specified range
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Common Problem 1
• Insufficient data
• Minimum three (3)
levels of concentration
in triplicates covering
the specified range

Common Problem 2
• No confidence
interval
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√
 Type of analytical Identification Testing For Impurities Assay
procedure Quantitation Limit - dissolution
characteristics (measurement only)
- content/ potency
Accuracy - + - +

Precision
Repeatability - + - +

Interm. Precision - + (1) - + (1)

Specificity (2) + + + +

Detection Limit - - (3) + -

Quantitation Limit - + - -

Linearity - + - +

Range - + - +

- signifies that this characteristic is not normally evaluated

+ signifies that this characteristic is normally evaluated
(1) in cases where reproducibility has been performed, intermediate precision is not needed
(2) lack of specificity of one analytical procedure could be compensated by other supporting analytical
procedure(s)
(3) may be needed in some cases
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Precision

The precision of an analytical procedure expresses

closeness of agreement (degree of scatter) between
a series of measurements obtained from multiple
sampling of the same homogeneous sample under
the prescribed conditions

ICH Q2(R1) Guideline, 1996

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Precision
• Same operating conditions
Repeatability • Over a short period of time
• intra-assay precision

• Within laboratory variations:

Intermediate • *diff analyst
precision • *diff days
(Ruggedness) • *diff equipment

Reproducibility • between laboratories

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Precision
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Repeatability
Data Required Testing Method
 Testing Method  Minimum three (3) levels

 Acceptance criteria of concentration in

triplicates covering the
 Raw data in tabulated
specified range
form
OR
 standard deviation,
relative standard  minimum six (6) replicates

deviation (coefficient of at 100% of the working

variation) and confidence concentration
interval should be
reported for each type of Acceptance criteria
precision investigated
 RSD ≤ 2.0% (sample soln)
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Common Problem 1
• Insufficient data
• minimum six (6)
replicates at
100% of the
working
concentration

Common Problem 2
• No confidence
interval
Confidence interval ???
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Intermediate Precision
Data Required Testing Method
 Testing Method  Variation of analyst, date,

 Acceptance criteria equipment (at least 2

parameter
 Raw data in tabulated
form
 standard deviation, Acceptance criteria
relative standard  RSD ≤ 2.0%
deviation (coefficient of
variation) and confidence
interval should be
reported for each type of
precision investigated
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Common Problem 1

• Intermediate precision not provided

 Common
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Problem 2
• Insufficient
data

Common
Problem 3
• Confidence
interval not
provided
Confidence interval ???
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Accuracy vs Precision
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Thank you