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How To Make Calibration Curve

The document provides instructions for creating a calibration curve from concentration and absorbance data: 1) Enter the concentration and absorbance data into a table 2) Select the data and insert a scatter chart 3) Change the chart title to "Calibration Curve" and add axis titles for concentration and absorbance 4) Insert a trendline and include the equation and R2 value to analyze the linearity of the calibration curve.

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Adibah Adawiyah
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101 views3 pages

How To Make Calibration Curve

The document provides instructions for creating a calibration curve from concentration and absorbance data: 1) Enter the concentration and absorbance data into a table 2) Select the data and insert a scatter chart 3) Change the chart title to "Calibration Curve" and add axis titles for concentration and absorbance 4) Insert a trendline and include the equation and R2 value to analyze the linearity of the calibration curve.

Uploaded by

Adibah Adawiyah
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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1 Insert data for x and y axis in a table

Concentration
A250 (y)
ug/ml (x)
0 0.002
Do not include
10 0.168 data for sample as
20 0.329 you need to
30 0.508 calculate it later.
40 0.66
50 0.846

2 Highlight all the data in the table > Insert >


Charts (Scatter)

Chart Title
1
0.8
0.6
0.4
0.2
0
0 10 20 30 40 50 60
3 Change Chart Title by clicking on the "Chart Title" and type the actual title.

Calibration Curve
Absorbance (250 nm) 1

0.8

0.6

0.4

0.2

0
0 10 20 30 40 50 60
Concentration, ug/ml

4 Insert Axis Title


Click Chart > Design > Add Chart Element > Axis Title > Primary Horizontal
& Primary Vertical

Calibration Curve
Absorbance (250 nm)

0.5

0
0 10 20 30 40 50 60
Concentration, ug/ml
5 Insert Trendline, Equation and R2
Click chart > Click + on the right > Click Trendline > Click arrow and More
Options > Scroll down and Click Equation and R2

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