Properties of Lipids Eclevia

NUEVA ECIJA UNIVERSITY OF SCIENCE AND TECHNOLOGY
COLLEGE OF ARTS AND SCIENCES

COLLEGE OF NURSING
BIOCHEMISTRY (MC2) LABORATORY
POST LABORATORY REPORT IN
PROPERTIES OF LIPIDS
ROXANNE S. ECLEVIA
BSN 1 – A
OCTOBER 29, 2019
INTRODUCTION
Lipids are organic compounds that contain hydrogen, carbon, and oxygen atoms, which forms the
framework for the structure and function of living cells. These organic compounds are nonpolar molecules,
which are soluble only in nonpolar solvents and insoluble in water because water is polar molecules. In the
human body, these molecules can be synthesized in the liver and are and generally found in the oil, butter,
whole milk, cheese, fried foods, and also in some red meats. The difference between fats and oils is on the
basis of their physical states at room temperature. It is customary to call a lipid a fat if it is solid at 25°C, and an
oil if it is a liquid at the same temperature.
It is established that lipids play extremely important roles in the normal functions of a cell. Not only do
lipids serve as highly reduced storage forms of energy, but they also play an intimate role in the structure of
cell membrane and organellar membranes. Lipids perform many functions, such as: Energy Storage, Making
Biological Membranes, Insulation, Protection – e.g. protecting plant leaves from drying up, Boyancy, Acting as
hormones, Act as the structural component of the body and provide the hydrophobic barrier that permits
partitioning of the aqueous contents of cell and sub cellular structures. Lipids are major sources of energy in
animals and high lipid-containing seeds and activators of enzymes.
METHODS
IN SEPARATE TEST TUBES

PLACE 1 ML OF WATER,
DILUTE HCl, DILUTE + 2 DROPS OF EACH TEST OBSERVE IN WHAT
NaOH, ETHYL ALCOHOL, SAMPLE IN EACH TEST SOLVENT IS THE OIL
CHLOROFORM, ETHER TUBE SOLUBLE.
AND CARBON
TETRACHLORIDE
Figure 1. Solubility Test
ON THE OTHER SIDE OF AFTER 15 MINS, NOTE

THE SAME FILTER PAPER, THE APPERANCE OF THE
PUT A DROP OF TEST
PUT A DROP OF ETHYL FILTER PAPER. NOTE
SOLUTION ON ONE SIDE
ALCOHOL AND ON THE WHICH WILL LEAVE A
OF FILTER PAPER.
OTHER CORNER A DROP SPOT AND WHICH WILL
OF ETHER. LABEL EACH. NOT.
POST LABORATORY REPORT IN BIOCHEMISTRY (MC2) rtae

Figure 2. Spotting Effect
PLACE 1 ML OF ETHYL
TEST THE SOLUTION
ALCOHOL IN SEPARATE
WITH RED AND BLUE
TEST TUBES + 5 DROPS
LITMUS PAPER
OF TEST SAMPLES
Figure 3. Reaction with Litmus Paper
PLACE 1 ML OF TEST HEAT IN WATER BATH

+ A PINCH OF
SAMPLE IN SEPARATE OBSERVE AND DESCRIBE
POTASSIUM BISULFATE
TEST TUBES THE ODOR PRODUCED
Figure 4. Acrolein Test
+ DROPWISE OF A
SOLUTION OF Br2 IN CCl4
PLACE 5 DROPS OF TEST
TO EACH, COUNT THE
SAMPLES IN 1ML OF
NO. OF DROPS REQUIRED
CHLOROFORM
TO PRODUCE REDDISH
SOLUTION
Figure 5. Test for Unsaturation
+ 15 DROPS OF ACETIC
PLACE 5 DROPS OF TEST ANHYDRIDE, MIX
SAMPLES AND + MIX AND NOTE THE
CHLOROFORM UNTIL IT IS + 5 DROPS OF COLOR CHANGES
DISSOLVED CONCENTRATED
SULFURIC ACID
Figure 6. Liebermann-Burchard Test
RESULTS
TEST DILUTE DILUTE ETHYL CHLOROFORM ETHER CARBON WATER

SAMPLE HCl NaOH ALCOHOL TETRACHLORIDE
Butter Not Not Not Soluble Soluble Soluble Not
Soluble Soluble Soluble Soluble
Canola Not Not Not Soluble Soluble Soluble Not
Coconut Not Not Not Not Soluble Soluble Soluble Not
Palm Not Not Not Soluble Soluble Soluble Not
Lauric Not Not Not Soluble Soluble Soluble Not
Table 1. Solubility Test Results

TEST SAMPLE SPOTTING LIEBERMANN- REACTION WITH ACROLEIN TEST
EFFECT BURCHARD TEST LLITMUS PAPER
Butter Spot is Visible DARK GREEN BLUE: No No burnt smell
(Change in Color)Changes
RED: No Changes
Canola Spot is Visible GREEN (Change BLUE: No No burnt smell
in Color) Changes
RED: No Changes
Coconut Spot is Visible GREEN (Change BLUE: No No burnt smell
in Color) Changes
RED: No Changes
Palm Spot is Visible DARK GREEN BLUE: No No burnt smell
(Change in Color) Changes
RED: No Changes
Lauric Spot is Visible GREEN (Change BLUE: No No burnt smell
in Color) Changes
RED: No Changes
Water Not Visible GREEN (Change BLUE: No No burnt smell
in Color) Changes
RED: No Changes
Table 2. Results of other tests performed.
CONCLUSION
DISCUSSION AND INTERPRETATION OF THE DATA GATHERED IN RELATION TO THE GIVEN
BACKGROUND IN THE INTRODUCTION MUST BE REFLECTED IN THIS PART. A LOT ONE PARAGRAPH
EACH FOR EVERY PART OF THE EXPERIMENT. THERE IS NO LIMITATION AS TO HOW MANY
SENTENCES MAY BE USED. BE COHERENT IN CONSTRUCTING YOUR CONCLUSION.
REFERENCE:
To
https://microbenotes.com/lipids-properties-structure-classification-and-functions/
https://byjus.com/biology/lipids/

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NUEVA ECIJA UNIVERSITY OF SCIENCE AND TECHNOLOGY

COLLEGE OF ARTS AND SCIENCES

oil if it is a liquid at the same temperature.

animals and high lipid-containing seeds and activators of enzymes.

IN SEPARATE TEST TUBES

Figure 1. Solubility Test

ON THE OTHER SIDE OF AFTER 15 MINS, NOTE

POST LABORATORY REPORT IN BIOCHEMISTRY (MC2) rtae

Figure 3. Reaction with Litmus Paper

PLACE 1 ML OF TEST HEAT IN WATER BATH

Figure 4. Acrolein Test

Figure 5. Test for Unsaturation

Figure 6. Liebermann-Burchard Test

TEST DILUTE DILUTE ETHYL CHLOROFORM ETHER CARBON WATER

POST LABORATORY REPORT IN BIOCHEMISTRY (MC2) rtae

DISCUSSION AND INTERPRETATION OF THE DATA GATHERED IN RELATION TO THE GIVEN

SENTENCES MAY BE USED. BE COHERENT IN CONSTRUCTING YOUR CONCLUSION.

POST LABORATORY REPORT IN BIOCHEMISTRY (MC2) rtae

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