Medical Laboratory Report: MR S.Dileep
Medical Laboratory Report: MR S.Dileep
Medical Laboratory Report: MR S.Dileep
MOLECULAR BIOLOGY
SARS-CoV-2(COVID-19),Qualitative
(Method: Real Time-Polymerase Chain Reaction(RT-PCR))
Note:1)< 35 Ct Detected, > 35 Ct Not Detected. Test sample whose Ct value is beyond 35 must be
re-purified and repeat the test after 3-5 days with fresh sample for confirmation.
2)Test sample whose Ct value is nearly 35 must be re-purified and repeat the test after 3-5 days with
fresh sample for confirmation. Kindly correlate clinically.
CoVid-19 Real Time PCR Detection Kit is designed for the diagnosis of SARS-CoV-2 in respiratory
samples. The detection is done in one step real time Reverse Transcriptase format where the reverse
transcription and the subsequent amplification of specific target sequence occur in the same reaction
well. The isolated RNA target is transcribed generating complementary DNA by reverse transcriptase
which is followed by the amplification of a conserved region of ORF1ab and N genes for SARS-CoV-2
using specific primers and a fluorescent-labeled probe.In a real time PCR assay a positive reaction is
detected by accumulation of a fluorescent signal.Cycle Threshold value(Ct value).Ct levels are
inversely proportional to the amount of target nucleic acid in the sample (ie the lower the Ct level the
greater the amount of target nucleic acid in the sample)
Dr.S.M.DEVARA
Ch.Samuel
MD MICROBIOLOGIST
Manager Technical
This is an electronically authenticated report. Report printed date : 25/05/2021 22:33 Page 1 of 1
NOTE : Assay results should be correlated clinically with other clinical findings and the total clinical status of the patient.
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