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Shaheed Mohta Shaheed Mohtarma Benazir Bhutto Lab Lyari Lab Lyari

This document is a lab report from the Molecular Pathology Laboratory at Shaheed Mohtarma Benazir Bhutto Medical College in Lyari, Karachi. It reports that a SARS-CoV-2 RNA test for a 52-year-old male named M. Suleman was negative. The test used real-time PCR to detect SARS-CoV-2 RNA in a nasopharyngeal/oropharyngeal swab sample. Possible reasons for a negative result in an infected individual are listed.

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Muhammad Tahir
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102 views1 page

Shaheed Mohta Shaheed Mohtarma Benazir Bhutto Lab Lyari Lab Lyari

This document is a lab report from the Molecular Pathology Laboratory at Shaheed Mohtarma Benazir Bhutto Medical College in Lyari, Karachi. It reports that a SARS-CoV-2 RNA test for a 52-year-old male named M. Suleman was negative. The test used real-time PCR to detect SARS-CoV-2 RNA in a nasopharyngeal/oropharyngeal swab sample. Possible reasons for a negative result in an infected individual are listed.

Uploaded by

Muhammad Tahir
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Shaheed Mohta

Mohtarma Benazir Bhutto Lab Lyari


Molecular Pathology Laboratory
Add: New Building Shaeed Mohtrma Benazir Bhutto Medical Collage
Collage,, Tennery Road,
Lyari, Karachi.

Name: M.SULEMAN S/o, W/o, D/o: GOHAR AMAN CNIC: 42301-2083548-9

Age/Gender: 52/ MALE MR/ LAB NO: 6046-11-2020 Sample 13-12-2020


Received:
Reporting 12-12-2020 Referred By: Prof Dr. Anjum Location: SMBBMC LYARI
Date: Rehman
Principal SMBBMC

19) = “NEGATIVE”
Result : SARS-COV-2 RNA (COVID-19)

Test Name : SARS-CoV-2 RNA(( COVID


COVID-19) RNA DETECTION BY PCR (QUALITATIVE)

Sample : Nasopharyngeal/Oropharyngeal Swab

Principle: The Real –Time


Time PCR Coronavirus (COVID
(COVID-19) assay is an in Vitro diagnostic test based on

Fluorescence-based
based detection of specific SARS
SARS-CoV—2 targets in the viral genome.

Method:

The test was performed after RNA extraction using automated RNA extraction machines and amplified
using real-time
time PCR machines ( BioRad CFX
CFX-96/ABI
96/ABI QauntStudio 5). The Following controls were included
include :
RNA extraction control, PCR positive and negativ
negative controls.

Comments: A number of factors could lead to a negative result in an infected individual including,

 Poor Quality of specimen, containing little patient material.


 The Specimen was collected late or very early in infection.
 The specimen was not handle
handled and shipped appropriately.
 Technical reasons inherent
erent in the test, e.g. Virus Mutations in Primer Probe target region.

This is a System generated report does no


not required signature

Printed by: Muhammad TABISH JAVEED

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