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Ultracentrifugation and electrophoresis are techniques used to separate molecules based on their properties. [1] Ultracentrifugation spins molecules at high speeds, separating them based on density and mass. [2] Electrophoresis uses an electric current to separate molecules like DNA, RNA, or proteins based on their size and electrical charge. Both techniques rely on the inherent properties of molecules to achieve separation.

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74 views2 pages

Graphic Organizer

Ultracentrifugation and electrophoresis are techniques used to separate molecules based on their properties. [1] Ultracentrifugation spins molecules at high speeds, separating them based on density and mass. [2] Electrophoresis uses an electric current to separate molecules like DNA, RNA, or proteins based on their size and electrical charge. Both techniques rely on the inherent properties of molecules to achieve separation.

Uploaded by

rheena espiritu
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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has

ULTRACENTRIFUG ELECTROPHORES
ATION IS

 Invented by Theodor  Developed by Arne


Svedberg in 1925 Tiselius during the 1930s
 Used to separate
molecules having different  Used to separate DNA,
 Use in biology,
densities by spinning them RNA, or protein
biochemistry and
in solution around an axis molecules based on their
polymer science
at high speed size and electrical charge.
 Needs their basic
 The denser a biological components to  Under the effect of steady
structure is, the faster it function. current, negatively charged
settles. On the other hand, DNA molecules migrate
if more massive a biological  Both of them has a towards the positive charge,
particle is, the faster it principle that can resulting in separation based
goes. affect the result of the on DNA mass and charge.
process.
 Ultracentrifugation basic  Electrophoresis basic
components are solution components are the mixture
based on their size and of DNA, RNA or proteins.
density
References:

 Mendes, A. (2020, December 5). Ultracentrifugation basics and applications. Conduct Science.
https://conductscience.com/ultracentrifugation-basics-and-applications
 Narwat, P. S. (n.d.). Electrophoresis. SlideShare. Retrieved June 16, 2021, from
https://www.slideshare.net/PradeepNarwat/electrophoresis-152440572
 Centrifugation - an overview | ScienceDirect Topics. (n.d.). Science Direct. Retrieved June 16, 2021, from
https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/centrifugation
 Electrophoresis. (n.d.). Genome.Gov. Retrieved June 16, 2021, from https://www.genome.gov/genetics-
glossary/Electrophoresis#:%7E:text=Electrophoresis%20is%20a%20laboratory%20technique,move%20faster%20than
%20larger%20molecules
 Chauhan, T. (2020, May 19). Agarose gel electrophoresis. Genetic Education. https://geneticeducation.co.in/agarose-gel-
electrophoresis
 Attention Required! | Cloudflare. (n.d.). Toppr. Retrieved June 16, 2021, from https://www.toppr.com/ask/question/what-is-
gel-electrophoresis-explain-how-dna-fragments-are-separated-and-detected-using-this

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