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Experiment On Isolation of DNA

1. This document describes an experiment to extract DNA from plant material using common household chemicals and equipment. 2. The required materials include table salt, liquid detergent, distilled water, ethanol, test tubes, a beaker, and a water bath set to 90°C. 3. The procedure involves grinding plant material, adding salt solution, detergent, and ethanol, then heating and centrifuging the mixture to isolate and precipitate the extracted DNA.

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50% found this document useful (2 votes)
2K views3 pages

Experiment On Isolation of DNA

1. This document describes an experiment to extract DNA from plant material using common household chemicals and equipment. 2. The required materials include table salt, liquid detergent, distilled water, ethanol, test tubes, a beaker, and a water bath set to 90°C. 3. The procedure involves grinding plant material, adding salt solution, detergent, and ethanol, then heating and centrifuging the mixture to isolate and precipitate the extracted DNA.

Uploaded by

TRP Om
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Expt. No.

Page No Dated

EnpnumAnt Ne.
3

Tt _
AAALatsn Al DNA rLen awailahlL
plant matonial

T JAALato DNA Area. auoiLohla


oLant matuial wudh as Apinath
gnRAOALdspopaohonnna

Rqinomant
Bake table Ma l Liauuiddotinaat,
diatilld watuupipotto aLaALAninsa
lassALo ilto panu
tAt tube uan 90: thylalcoh sl2
PHadune
ul taok halanipandpulad
banana inte a bLakn. andodded
50meR tuatim Ruid (a54
tahlAalt + IDmALiquideeiA
nt 90 mldistilLd. watu.ul
LacsdL the haak i a wata hsta
t ath0°C AAL5 minutiuTAinu
tiuditAint uuih Lassned-
w ltaro5msceslod Lentant
Teacher's Signature
Expt. No. Page No Dated

ntsAslean tiut tub andadded


5mlAk Lold. 90/ than ol.
*OhAAAVatimA
AhinL h t DNA Can be ALgnat
th uactie al sALutia and
thanol.Tau DNAJLpawAnia h
DNA eund in plantcLA TKe.
2ADCIAA And.the DNdatA paacipitated
d A o shonisalt dtonoiat
*RAsAult
DAZApplanA As laitpRipie
tat AKuy i thuLads eapsa
Pcautiens.
E A h plant m a t i a s ahAuLd Jbe
ALd Jon AtHactimA DNA
2Th LasAuLAHLA aAAL SI theOugh
aansdandduld bala thi
pLnimontsAtat
Th nznA and heaicalsuALo
AtandLand. quality-
a uslddistilerd wat te aake
thau0lutied
Teacher's Signature:
Expt. No.
Page No Dated

5 hmisalA and Oluutiems ubsanld


upaLdcaneAally inSden te
audid. anu wastog

Teacher's Signature

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