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Research Plan Extraction of Sweet Potato

Research

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Grace Saagundo Dubduban
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17 views4 pages

Research Plan Extraction of Sweet Potato

Research

Uploaded by

Grace Saagundo Dubduban
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Research Plan

Chapter 1.

A. Statement of the Problem

The main problem of the study is to test the antibacterial property of sweet
potato leaves to Staphylococcus Aureus and Escherichia Coli. This research is used to
compare the extracts of sweet potato leaves and chloramphenicol in terms of inhibition
to E. Coli and S. Aureus.

Specifically it sought to answer the following queries:

1.Is the extract of sweet potato an effective agent against E. Coli and S.

Aureus?

2. Is the extract of sweet potato comparable to chloramphenicol?

3. Is there a significant difference between the potato leaves extract in the zone of
inhibition to E. Coli and S. Aureus?

B. Statement of hypotheses:

H1:

1. The extract of sweet potato is an effective antibacterial agent to E. Coli and S.


Aureus.

2. The extract of sweet potato is comparable to chloramphenicol.

3. There is a significant difference between the sweet potato leaves extract in


terms of inhibition to E. Coli and S. Aureus.

Ho:

1. There is no significant difference between the sweet potato leaves extract in


terms of inhibition to E. Coli and S. Aureus.

C. Conceptual Framework

A comparative study of the antibacterial potency of sweet potato and


pharmaceutical medicine, chloramphenicol against Staphylococcus aureus and
Escherichia Coli.

Research Plan

Extraction of Sweet Potato


Quality culture of E. Coli and Antibacterial potency in
S. Aureus Phytochemical Screening terms of inhibition and
bacterial time source of
E.coli and S. Aureus

D. Significance of the study


Result of the Study
Plants are important to the lives of people throughout the world. We
depend upon plants to satisfy some of our basic human needs as food, clothing, shelter,
and health care. Increasing attention has also been given to the role of plants in disease
management and alternative synthetic medicines. Different pathogens and bacteria are
now immune to a wide range of synthetic medicines, thus alternative medicines from
plants are greatest concern to replace it. The investigation stresses the possibility of
extraction a new, cheaper and readily available herbal medicines from Ipomea Batatas
which is not very known for its any pharmacological importance in the country,
therefore provide great savings on the part of Filipinos mostly in poverty line. Similarly,
it could provide potential source of income when large scale production if feasible.

E. Scope and Limitations

This study aims to assess the antibacterial potency of sweet potato against
two bacteria E. Coli and S. Aureus.

F. Definition of terms

Sweet potato- The sweet potato is a dicotyledonous plant that belongs to the morning
glory family Convolvulaceae. Its large, starchy, sweet-tasting, tuberous roots are a root
vegetable

Antibacterial- active against bacteria.

Staphylococcus aureus- Staphylococcus aureus is a gram-positive, round-shaped


bacterium that is a member of the Firmicutes, and is frequently found in the nose,
respiratory tract, and on the skin.

Escherichia Coli- Escherichia coli is a Gram-negative gammaproteobacterium commonly


found in the lower intestine of warm-blooded organisms.

Chapter 2
Review of related Literature

Sweet potato plant, which is native to tropical America, is cultivated on sandy or


loamy soils and exists as an important food staple in a number of countries. It is
planted primarily for its thick, edible roots, called sweet potatoes. Two main
types are commonly cultivated: a dry, mealy type; and a soft, light-to-deep-
yellow, moist-fleshed type. The species called wild sweet potato vine, man root,
or man-of-the-earth is not edible, but it is cultivated as an ornamental vine. The
sweet potato yields an important starch, which is used commercially in
laundries, in sizing textiles and papers, and in manufacturing adhesives. The
pink and yellow varieties are rich in carotene, the precursor of vitamin A. A 100
g root is reported to contain from 108 to 121 calories, 68.5 g to 72.3 g water, 1 g to
1.7 g protein, 0.2 g to 0.4 g fat, 25.6 g to 31.0 g total carbohydrate, 0.7 g to 1.0 g
ash, 21 mg to 36 mg Ca, 38 mg to 56 mg P, 0.7 mg to 2.0 mg Fe, 10 mg to 36 mg
Na, 210 mg to 304 mg K, 35 u.g to 5,280 lig beta-carotene equivalent, 0.09 mg to
0.14 mg thiamine, 0.04 mg to 0.06 mg ribo-flavin, 0.6 mg to 0.7 mg niacin, and 21
mg to 37 mg ascorbic acid. The peptic substances (total, 0.78: soluble, 0.43
percent) present in fresh tubers contain: uronic acid, 60; and methoxyl, 4 percent
to 5 percent. Other constituents in the tubers: phytin (1.05 percent), two mono-
amino-phosphatides (probably lecithin and cephalin), organic acids (oxalic acid),
phytosterolin, phytosterol, resins, tan-nins, and coloring matter. Sweet potato
contains calcium, 30; magnesium, 24: potassium, 373; sodium, 13; phospho-rus,
49; chlorine, 85; sulphur, 26: iron 0.8mg/100g; iodine, 4.51.1g/kg; man-ganese,
copper and zinc are present in traces (Hug et al., 1983). Sweet potato contains
raffinose. one of the sugars responsible for flatulence. Three of the sugars that
occur in plant tissues—raffinose, stchyose, and verbascose—are not digested in
the upper digestive tract, and so fer-mented by colon bacteria to yield flatus
gases. hydrogen, and carbon dioxide. (Palmer. 1982) S. aureus is found in the
skin and nostrils of many healthy individuals. These bacteria often give rise to
minor superficial diseases, such as the formation of pustules or boils in hair
follicles. Much more rarely, S. aureus can give rise to more serious infections;
these normally occur when the resistance of a tissue or the host is reduced. S.
aureus infection is char-acterized by the presence of pus and formation of
abscesses. This form Of staphylococcus is responsible for skin pustules, boils and
carbuncles, impeti-go, infections of the wound and burns, breast abscesses,
whitlow, osteomycli-tis, bronchopneumonia, septicemia, acute endocaditis, food
poisoning, and scalded skin syndrome. E. coli, a normal inhabitant in human
intestinal tract, is the most thoroughly studied organism. Studies of the
mechanisms of genetic exchange and the biology of plasmids and bactriophages
of E. coli have been crucial in understanding many aspects of DNA replication
and the expression of genetic material. These studies have led to the ability to
insert DNA from unrelated organisms into E. coli plas-mids and bacteriophages,
and to have that DNA replicated by the bacteria, with the genetic information it
contains expressed by the bacteria. It is thus possible for bacteria to become
living factories for scarce biological products such as human insulin, interferon,
and growth hormone (Microsoft Encarta Encyclopedia: 2002).

Chapter 3

Methodology

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