Scribd Logo
Upload

Welcome to Scribd!

  • 7 views

    Microscopy and Cells

    Uploaded by

    Farah Saleh
    The document discusses an experiment where radioactive amino acids were used to track protein trafficking between organelles in a tissue over time. The percentages of radioactivity found in vesicles, the rough endoplasmic reticulum (rER), and the Golgi apparatus were recorded at several time points. Based on the data, it was determined that the vesicles containing radioactivity came from the Golgi apparatus rather than the rER. The decrease in total radioactivity between the 5 and 10 minute time points was attributed to radioactive amino acids being in transit between organelles. Reliability of the data is limited since measurements were not continuous.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Microscopy and Cells

    Uploaded by

    Farah Saleh
    7 views6 pages
    The document discusses an experiment where radioactive amino acids were used to track protein trafficking between organelles in a tissue over time. The percentages of radioactivity found in vesicles, the rough endoplasmic reticulum (rER), and the Golgi apparatus were recorded at several time points. Based on the data, it was determined that the vesicles containing radioactivity came from the Golgi apparatus rather than the rER. The decrease in total radioactivity between the 5 and 10 minute time points was attributed to radioactive amino acids being in transit between organelles. Reliability of the data is limited since measurements were not continuous.

    Original Title

    microscopy and cells

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    The document discusses an experiment where radioactive amino acids were used to track protein trafficking between organelles in a tissue over time. The percentages of radioactivity found in vesicles, the rough endoplasmic reticulum (rER), and the Golgi apparatus were recorded at several time points. Based on the data, it was determined that the vesicles containing radioactivity came from the Golgi apparatus rather than the rER. The decrease in total radioactivity between the 5 and 10 minute time points was attributed to radioactive amino acids being in transit between organelles. Reliability of the data is limited since measurements were not continuous.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    7 views6 pages

    Microscopy and Cells

    Uploaded by

    Farah Saleh
    The document discusses an experiment where radioactive amino acids were used to track protein trafficking between organelles in a tissue over time. The percentages of radioactivity found in vesicles, the rough endoplasmic reticulum (rER), and the Golgi apparatus were recorded at several time points. Based on the data, it was determined that the vesicles containing radioactivity came from the Golgi apparatus rather than the rER. The decrease in total radioactivity between the 5 and 10 minute time points was attributed to radioactive amino acids being in transit between organelles. Reliability of the data is limited since measurements were not continuous.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 6

    Dr.

    Jastrow Electron microscopy atlas

    http://www.drjastrow.de/WAI/EM/EMAtlas.html
    One way to investigate protein trafficking is to use radioactive amino acids.
    A tissue was soaked in a solution of radioactive amino acids for a short period of time and then transferred to a solution with non-radioactive
    amino acids.
    The table below shows the amount of radioactivity as a percentage of the total radioactivity of the total radioactivity in the cells of this tissue,
    found in three organelles, at three different times being transferred to the non-radioactive solution.

    Percentage of total radioactivity in cells after tissue transferred to non-


    Organelle radioactive solution
    5 min 10 min 45 min
    Vesicles 0 5 60
    rER 80 10 5
    Golgi apparatus 10 80 30
    Total radioactivity 90 95 95

    (a) The vesicles containing radioactivity can either come from rER or the Golgi apparatus. Using evidence from the table, suggest whether
    these vesicles come from rER or the Golgi apparatus.
    (b) Suggest an explanation for the difference in the total radioactivity between 5 minutes and 10 minutes.
    (c) Comment on the reliability of the data collected.
    (a)

    • Little radioactivity in vesicles at 5 and 10 minutes


    • High radioactivity in rER at 5 minutes and in Golgi apparatus at 10 minutes
    • Vesicles therefore come from Golgi apparatus

    (b)
    Data was collected at large intervals rather than continuously, hence some radioactive amino acids were in vesicles from in their way from rER
    to Golgi apparatus and were not sampled.
    Total is not 100% due to background losses

    (c)
    The data is collected at large intervals rather than continuously, so the data cannot be reliable enough. Use of a data logger may improve the
    reliability and capture radioactivity fully at all times.

    You might also like