Service Manual Beckman Coulter Maxm

COULTER® MAXM™ Hematology Analyzer
COULTER® MAXM™ Hematology Analyzer

with Autoloader
Service Manual
PN 4235961E (August 2000)

COULTER CORPORATION
A Beckman Coulter Company
Miami, Florida 33196-2500 USA
LEGAL NOTICES
Beckman Coulter, Inc. makes no representation that, upon furnishing this service manual, the holder of the manual will have
the necessary technical capabilities and know-how to properly troubleshoot and repair any of the equipment specified in the
manual. Beckman Coulter, Inc. assumes no liability whatsoever, including consequential and incidental damages, resulting
from improper operation of Beckman Coulter instruments after maintenance of Beckman Coulter instruments has been
performed by persons not employed by Beckman Coulter, Inc. Furthermore, Beckman Coulter, Inc. assumes no liability
whatsoever for any personal injury or property damage resulting from maintenance and/or repair of Beckman Coulter
instruments performed by persons not employed by Beckman Coulter, Inc.
READ ALL PRODUCT MANUALS AND CONSULT WITH BECKMAN COULTER-TRAINED PERSONNEL
BEFORE ATTEMPTING TO OPERATE INSTRUMENT.
HAZARDS AND OPERATIONAL PRECAUTIONS AND LIMITATIONS
WARNINGS, CAUTIONS, and IMPORTANTS alert you as follows:
WARNING - Might cause injury.

CAUTION - Might cause damage to the instrument.
IMPORTANT - Might cause misleading results.
Beckman Coulter, Inc. urges its customers to comply with all national health and safety standards such as the use of barrier
protection. This may include, but it is not limited to, protective eyewear, gloves, and suitable laboratory attire when
operating or maintaining this or any other automated laboratory analyzer.
"This Service Manual contains confidential information of Beckman Coulter, Inc. and its receipt or possession does not
convey any rights to reproduce, disclose its contents, or to manufacture, use, or sell anything it may describe.
Reproduction, disclosure, or use without specific written authorization of Beckman Coulter, Inc. is strictly forbidden."
Copyright © Beckman Coulter, Inc. 1992-2000

All rights reserved.
REVISION STATUS
Initial Issue, 8/92
Revision B, 3/94
Software Version 7C
Complete revision.
Revision C, 6/96
Software Version 8B
Complete revision.
Revision D, 10/99
Released by CN 027360-1079
Software Version 8D
Complete Revision. Rearranged the contents to follow the format used in the COULTER® HmX
Hematology Analyzer Service Manual, removed information pertaining to the DMS, expanded and
updated the illustrated parts list and the quick reference information, and incorporated information
released by Service Bulletins SB 1286, SB 1315, SB 1321, and SB 1336, and by Service Memos SM 1499,
SM 1538, SM 1564, SM 1570, SM SM 1575, SM 1585, SM 1611, SM 1621, SM 1624, SM 1627, SM 1630,
SM 1636, SM 1643, SM 1658, SM 1660, SM 1673, SM 1675, SM 1677, SM 1679, SM 1690, SM 1702,
SM 1735, SM 1744, SM 1752, SM 1758, SM 1762B, SM 1769, SM 1777, SM 1778, SM 1779, SM 1793,
SM 1802, SM 1805, SM 1808, SM 1810, SM 1812 and 1847.
Revision E, 8/00
Released by CN 027360-1116
Software Version 8D
Revision E is being released as change pages (PN 4277103). This revision updates all needle-drive cylinder
illustrations to include the hazard label and the Hall effect needle-position sensors, adds a procedure for
aligning the needle-position sensors, updates the parts lists, and incorporates information released by
Service Memos 1621, 1821, and 1883.
Changes were made on the following pages 1.1-1, 1.1-3, 1.1-4, 1.2-3, 1.2-4, 2.1-1, 2.1-2, 2.8-2, 2.9-2,
2.9-3, 2.9-4, 3.2-2, 3.2-5, 3.6-2, 3.6-3, 3.10-1 through 3.10-5, 4.9-3, 4.11-1, 4.12-2, 4.13-2 and 4.13-3,
4.20-4, 4.30-1, 4.31-2, 4.34-2, 4.35-1, 4.38-1, 4.40-1, 4.42-1, 4.43-2, 4.43-5, 4.44-1 through 4.44-3, 7.1-7
and 7.1-8, 7.1-13, 7.1-24 (deletions), 8.1-3, 8.1-4, 8.1-5, 8.1-6, 8.1-7, 8.1-11, 8.1-14, 8.1-18, 8.1-25,
8.1-26, 8.2-3, 8.2-5, 8.2-12, 8.2-13, 8.2-14, 8.2-15, 8.2-19, 8.2-21, 8.2-23, 8.2-29, 8.2-30, 8.2-31, 8.2-34,
8.2-41, 8.2-45, A.5-2, A.5-3 through A.5-7.
The change page packet also includes the latest revision of the Pneumatic/Hydraulic Layout,
DCN 6320510.
Changes that are part of the most recent revision are indicated in the printed copy by a bar in the margin of
the amended page.
This document applies to the latest software listed and higher versions. When a subsequent software version affects the
information in this document, the changes will be included on minor revision change pages or summarized on a Notice of
Information Update form and will be released by service memo.
PN 4235961E iii
REVISION STATUS
iv PN 4235961E
CONTENTS
LEGAL NOTICES
REVISION STATUS, iii
1 INTRODUCTION, 1.1-1
1.1 MANUAL DESCRIPTION, 1.1-1

Scope, 1.1-1
Intended Audience, 1.1-1
Organization, 1.1-2
Numbering Format, 1.1-2
Special Headings, 1.1-3
WARNING, 1.1-3
CAUTION, 1.1-3
IMPORTANT, 1.1-3
ATTENTION, 1.1-3
Note, 1.1-3
Conventions, 1.1-4
1.2 SAFETY PRECAUTIONS, 1.2-1

Laser, 1.2-1
Laser Beam Hazards, 1.2-1
Laser Radiation Hazards, 1.2-2
Electronic, 1.2-3
Biological, 1.2-3
Troubleshooting, 1.2-4
2 INSTRUMENT DESCRIPTION, 2.1-1
2.1 INTRODUCTION TO THE COULTER MAXM ANALYZER, 2.1-1

Function, 2.1-1
Description, 2.1-1
Main Components, 2.1-1
Systems, 2.1-4
Physical Specifications, 2.1-4
Performance Specifications and Characteristics, 2.1-4
Leakage Current Specifications, 2.1-5
Operation, 2.1-5
Modes of Operation, 2.1-5
Operating Procedures, 2.1-6
2.2 ELECTRONIC SYSTEM - OVERVIEW AND POWER SUPPLIES, 2.2-1

Electronic System Overview, 2.2-1
Electronic Power Supply, 2.2-1
Components, 2.2-1
Function, 2.2-2
Color Coding, 2.2-3
Connectors, 2.2-4
Specifications, 2.2-4
Voltage Configurations, 2.2-4
Fuses, 2.2-4
PN 4235961E v
CONTENTS
Laser Power Supply, 2.2-4

Function, 2.2-4
RF Power Supply, 2.2-4
Components, 2.2-4
Function, 2.2-4
Voltage Monitoring, 2.2-4
2.3 ELECTRONIC SYSTEM - SIGNAL FLOW BLOCK DIAGRAMS, 2.3-1
2.4 ELECTRONIC SYSTEM - ANALYZER MODULE, 2.4-1

Function, 2.4-1
Description, 2.4-1
376 CPU Card, 2.4-2
Description, 2.4-2
Software Download Sequence, 2.4-2
Jumpers for the 376 CPU Card, 2.4-3
376 CPU Card Inputs/Outputs, 2.4-3
RAM Timer Card, 2.4-3
Jumpers for the RAM Timer Card, 2.4-3
DILUTER INTERFACE Card, 2.4-4
Function, 2.4-4
Lyse Trigger Test Point, 2.4-4
Jumper, 2.4-4
DILUTER INTERFACE Card Inputs/Outputs, 2.4-4
I/O Card, 2.4-6
Function, 2.4-6
Jumpers, 2.4-7
I/O Card Inputs/Outputs, 2.4-7
VCS PROCESSOR Card, 2.4-8
Function, 2.4-8
Noise Levels, 2.4-9
Jumper, 2.4-9
VCS PROCESSOR Card Inputs/Outputs, 2.4-9
R/W/P PROC Card, 2.4-9
Function, 2.4-9
RBC and WBC Counting Sequences, 2.4-10
RBC and WBC Voting Criteria, 2.4-10
WBC, RBC and Plt Histogram Development, 2.4-10
Data Transfer Method for Histogram Development, 2.4-10
Voting Criteria for Histogram-Derived Parameters, 2.4-13
Noise Levels, 2.4-13
Jumpers, 2.4-13
R/W/P PROC Card Inputs/Outputs, 2.4-13
R/W PREAMP Card, 2.4-14
Function, 2.4-14
R/W PREAMP Card Inputs/Outputs, 2.4-14
vi PN 4235961E
CONTENTS
2.5 PNEUMATIC SYSTEM, 2.5-1

Pneumatic Power Supply, 2.5-1
Components, 2.5-1
Function, 2.5-1
Fuse, 2.5-2
Pressure and Vacuum Distribution, 2.5-2
60-psi Pressure Distribution, 2.5-2
Sheath Pressure Distribution, 2.5-3
Sample Pressure Distribution, 2.5-3
High Vacuum Distribution, 2.5-3
Low Vacuum Distribution, 2.5-3
Pneumatic Monitor Card, 2.5-3
2.6 REAGENT SYSTEM, 2.6-1

Reagent Input and Distribution, 2.6-1
Reagent Monitoring, 2.6-1
Fluid Detector/Ram Pressure Card, 2.6-1
Function, 2.6-2
Connectors, 2.6-2
Reagent Temperature Control, 2.6-3
Peltier Module Description, 2.6-3
Peltier Module Function, 2.6-3
Temperature Range Cycles, 2.6-4
Waste Removal and Monitoring, 2.6-5
2.7 SAMPLE HANDLER SYSTEM - SAMPLE HANDLER CARD, 2.7-1

Function, 2.7-1
LEDs, 2.7-1
Responses During Power Up, 2.7-1
Responses During Download, 2.7-3
Responses to the Carousel Position in the Rotary Cap-Pierce Module, 2.7-4
Response to a Power Fluctuation, 2.7-4
Ensuring the Instrument and the Sample Handler Configurations Match, 2.7-4
Avoiding Instrument Damage During a Download, 2.7-4
Jumpers, 2.7-5
Sample Handler Card Inputs/Outputs, 2.7-5
2.8 SAMPLE HANDLER SYSTEM - ROTARY CAP-PIERCE MODULE, 2.8-1

Function, 2.8-1
Description, 2.8-1
Summary of Operation, 2.8-3
RCP Junction Card, 2.8-4
Function, 2.8-4
RCP Junction Card Inputs/Outputs, 2.8-5
2.9 SAMPLE HANDLER SYSTEM - AUTOLOADER MODULE, 2.9-1

Function, 2.9-1
Description, 2.9-1
PN 4235961E vii
CONTENTS

Autoloader Interface Card, 2.9-7
Function, 2.9-7
Autoloader Interface Card Inputs/Outputs, 2.9-8
Rocker Bed Interface Card, 2.9-8
Function, 2.9-8
Rocker Bed Interface Card Inputs/Outputs, 2.9-9
2.10 SAMPLE HANDLER SYSTEM - AUTOSENSOR TEST CARD, 2.10-1
2.11 SAMPLE PROCESSING SYSTEM - DILUTER, 2.11-1

Function, 2.11-1
Description, 2.11-1
Solenoid Valves, 2.11-1
Blood Sampling Valve (BSV), 2.11-2
Blood/Bubble Detectors, 2.11-3
Sample Processing, 2.11-4
Primary-Mode Aspiration, 2.11-4
WBC/RBC Sample Delivery, 2.11-5
Diff Segmentation, 2.11-6
Diff Segment and Erythrolyse II Reagent Delivery, 2.11-6
Cross Rinse to the Baths, 2.11-7
Secondary-Mode Aspiration, 2.11-7
Pre-Prep/Latex Aspiration, 2.11-8
RBC Sample Delivery, Secondary Mode, 2.11-9
Backwash Functions, 2.11-10
2.12 SAMPLE ANALYSIS SYSTEM - CBC TECHNOLOGY, 2.12-1

Summary, 2.12-1
Applying the Coulter Principle, 2.12-1
Sensing Area, 2.12-1
Aperture Current, 2.12-1
Aperture Vacuum, 2.12-2
Analyzing the RBC, WBC, and Plt Data, 2.12-2
Determining the Hgb, 2.12-2
2.13 SAMPLE ANALYSIS SYSTEM - VCS TECHNOLOGY, 2.13-1

Summary, 2.13-1
VCS Measurements, 2.13-1
V - Volume (DC), 2.13-1
C - Conductivity (RF), 2.13-1
S - Light Scatter (LS), 2.13-1
Opacity (OP), 2.13-2
Rotated Light Scatter (RLS), 2.13-2
Linear Light Scatter (LLS), 2.13-2
Applying the VCS Technology, 2.13-2
DC and RF Currents, 2.13-2
Laser Light Source, 2.13-2
viii PN 4235961E
CONTENTS
Flow-Cell Hydraulics, 2.13-3

Analyzing the Data, 2.13-5
Preprocessing, 2.13-5
RF Detector Preamp Card, 2.13-5
Function, 2.13-5
RF Detector Preamp Card Inputs/Outputs, 2.13-6
LS Preamp 5 Module, 2.13-6
Primary Function, 2.13-6
Mode Select, 2.13-7
LS Offset Voltage, 2.13-7
LS Preamp 5 Module Inputs/Outputs, 2.13-8
3 INSTALLATION PROCEDURES, 3.1-1
PART A: INSTRUMENT INSTALLATION
3.1 PREINSTALLATION CHECK, 3.1-1

Customer Training, 3.1-1
Carton Arrival, 3.1-1
Space and Accessibility, 3.1-1
Electrical Input, 3.1-1
Ambient Temperature and Humidity, 3.1-2
Ventilation, 3.1-2
Drainage, 3.1-3
Supplies, 3.1-3
3.2 INITIAL SETUP, 3.2-1
3.3 CONNECTING ASSEMBLIES, 3.3-1

Electronic Cable Connections, 3.3-1
Tubing Connections, 3.3-4
3.4 TESTING/CONFIGURING ASSEMBLIES, 3.4-1

Data Management System (DMS), 3.4-1
Software Installation, 3.4-1
Software Options Installation, 3.4-1
Bar-Code Reader Decoder Card Configuration, 3.4-1
Setting Up the Optional Printers, 3.4-1
3.5 INITIAL SYSTEM SETUP, 3.5-1

Setting Up the Institution Information, 3.5-1
Reagent System Setup, 3.5-1
Priming Reagents, 3.5-1
Adjusting the Hemoglobin Blank (Hgb Blank), 3.5-1
Adjusting the Blood/Bubble Detector Gains, 3.5-2
3.6 SYSTEM TESTING, 3.6-1

Tools/Supplies Needed, 3.6-1
Performing System Test and Start Up, 3.6-1
PN 4235961E ix
CONTENTS
Verifying General Operation of the Primary Mode on Instruments with a Rotary

Cap-Pierce Module, 3.6-2
Verifying General Operation of the Primary Mode on Instruments with an
Autoloader Module, 3.6-3
Verifying General Operation of the Secondary Mode, 3.6-5
3.7 ADJUSTMENTS AND CALIBRATION, 3.7-1

Entering Calibration Factors, 3.7-1
Measuring the RMS Noise, 3.7-1
Verifying the Diluent and Lytic Reagent Dispense Timing, 3.7-1
Verifying the CBC Latex Calibration, 3.7-1
Adjusting the Clog Detector Circuit, 3.7-1
Measuring the LS Current/LS Offset Voltage, 3.7-1
Verifying the DC Count and VCS Flow Rate, 3.7-2
Verifying the Diff and Retic Latex Calibration, 3.7-2
Checking Reproducibility and Carryover in the Primary Mode, 3.7-2
Checking Reproducibility and Carryover in the Secondary Mode, 3.7-2
Checking Reproducibility and Carryover for the Retic Parameter, 3.7-3
Making Initial Primary-Mode Calibration Adjustments, 3.7-3
Verifying the Secondary Mode - to - Primary Mode Calibration, 3.7-3
Calibrating the Primary Mode with S-CAL® Calibrator, 3.7-4
Setting Up the Control Files and Running the Controls, 3.7-4
Completing the Installation Paperwork, 3.7-4
3.8 ACCOUNT/INSTRUMENT INFORMATION, 3.8-1

Purpose, 3.8-1
Account Information, 3.8-1
Installation Test Data Checklist, 3.8-1
(c = confirmed complete, * = Retic units only), 3.8-1
Installation Test Data Log Sheets, 3.8-2
A. System Test Data, 3.8-2
B. Startup Results, 3.8-3
C. RMS Noise Check Measurements (CBC), 3.8-4
D. RMS Noise Check Measurements (Diff and Retic), 3.8-4
E. CBC-Mode Latex Calibration, 3.8-5
F. Clog Detector Setup Results, 3.8-6
G. Light Current/LS Offset Measurements, 3.8-6
H. Five Patient Scatterplots Displaying DC Counts and Times, 3.8-7
I. Diff-Mode Latex Calibration (Five Consecutive Runs), 3.8-8
J. Retic-Mode Latex Calibration (Five Consecutive Runs), 3.8-9
K. Primary-Mode Reproducibility Run, 3.8-10
L. Primary-Mode Carryover Run, 3.8-11
M. Secondary-Mode Reproducibility Run, 3.8-12
N. Secondary-Mode Carryover Run, 3.8-13
O. Retic Mode-to-Mode Carryover Run, 3.8-14
P. Retic Within-Mode Carryover Run, 3.8-15
Q. Primary-Mode Initial Adjustment to 5C Normal Cell Control Cal
Factors, 3.8-16
R. Mode-to-Mode Calibration and Verification Runs/Secondary Mode
Calibration Factors, 3.8-17
x PN 4235961E
CONTENTS
S. S-CAL Calibrator Calibration Batch Tables/Cal Factors, 3.8-18

T. Control Results, 3.8-19
PART B: UPGRADES AND OPTIONS INSTALLATION
3.9 Setting Up the Optional Printers, 3.9-1

Graphic Printer Installation, 3.9-1
Anadex Ticket Printer Installation, 3.9-1
3.10 CONFIGURING THE MAXM ANALYZER FOR CYANIDE-FREE

REAGENTS, 3.10-1
Purpose, 3.10-1
Hardware Changes, 3.10-1
Procedure, 3.10-1
Verification of the Hardware Changes, 3.10-3
Reagent Changes, 3.10-3
Pre-Conversion Instrument Verification, 3.10-3
Reagent Conversion, 3.10-4
Post-Conversion Instrument Verification, 3.10-4
4 SERVICE AND REPAIR PROCEDURES, 4.1-1
4.1 GUIDELINES FOR SERVICING THE MAXM ANALYZER, 4.1-1

General, 4.1-1
Power Down/Power Up the System, 4.1-2
Purpose, 4.1-2
Power Down, 4.1-2
Power Up, 4.1-2
Reset the System, 4.1-2
Clear the CPU RAM, 4.1-3
Purpose, 4.1-3
Procedure, 4.1-3
4.2 USING THE SERVICE DISK, 4.2-1

Purpose, 4.2-1
Activating the DMS Service Options, 4.2-1
Deactivating the DMS Service Options, 4.2-2
Using the Service Disk Software on a DMS, 4.2-3
Using the Service Disk Software on a Hard Drive, 4.2-5
Installing the Software, 4.2-5
Running the Software, 4.2-5
4.3 WHOLE BLOOD VERIFICATION - CBC/DIFF, 4.3-1

Purpose, 4.3-1
Procedure, 4.3-1
PN 4235961E xi
CONTENTS
4.4 LATEX CALIBRATION AND VERIFICATION, 4.4-1

Purpose, 4.4-1
CBC Latex Calibration and Verification, 4.4-1
Procedure, 4.4-1
Diff and Retic Latex Calibration and Verification, 4.4-2
Procedure, 4.4-2
4.5 REPRODUCIBILITY CHECKS, 4.5-1

Purpose, 4.5-1
Verifying Reproducibility in the Primary Mode, 4.5-1
Procedure, 4.5-1
Verifying Reproducibility in the Secondary Mode, 4.5-2
Procedure, 4.5-2
Verifying Retic % Reproducibility (Sample Analysis), 4.5-3
Procedure, 4.5-3
4.6 CARRYOVER CHECKS, 4.6-1

Purpose, 4.6-1
Checking Carryover in the Primary Mode, 4.6-1
Procedure, 4.6-1
Checking Carryover in the Secondary Mode, 4.6-2
Procedure, 4.6-2
Retic Carryover Tests, 4.6-2
General Instructions, 4.6-3
Retic Mode-to-Mode Carryover, 4.6-3
Retic Within Mode, 4.6-3
4.7 INITIAL ADJUSTMENT OF PRIMARY-MODE CALIBRATION FACTORS, 4.7-1

Purpose, 4.7-1
Procedures, 4.7-1
4.8 SECONDARY MODE-TO-PRIMARY MODE CALIBRATION, 4.8-1

Purpose, 4.8-1
Procedure, 4.8-1
4.9 VCS OPTIMIZATION, 4.9-1

Purpose, 4.9-1
A. Preliminary Checks, 4.9-3
B. Count Ratio Check, 4.9-3
C. VCS Flow-Rate Adjustment, 4.9-4
xii PN 4235961E
CONTENTS
D. Clog Detector Circuit Adjustment, 4.9-4

E. RF Detector Preamp Card C1 Adjustments, 4.9-4
F. DC, RF, and LS Gains Adjustments, 4.9-4
G. DC, RF and LS Noise Measurements, 4.9-4
H. Erythrolyse II and StabiLyse Reagent Pumps Volume Adjustments, 4.9-4
I. Verification, 4.9-4
4.10 VCS FLOW-RATE ADJUSTMENT, 4.10-1

Purpose, 4.10-1
Sheath Pressure Adjustment, 4.10-1
Sample Pressure Adjustment, 4.10-1
Verification, 4.10-3
4.11 RF DETECTOR PREAMP CARD ADJUSTMENTS, 4.11-1

Purpose, 4.11-1
Clog Detector Circuit Adjustment, 4.11-1
Procedure, 4.11-1
C1 Adjustment, 4.11-2
Procedure, 4.11-2
4.12 RMS NOISE CHECKS, 4.12-1

Purpose, 4.12-1
CBC RMS Noise Checks, 4.12-1
Procedure, 4.12-1
Diff and Retic RMS Noise Checks, 4.12-1
Procedure, 4.12-1
4.13 FLOW-CELL CLEANING, 4.13-1

Purpose, 4.13-1
Procedure, 4.13-1
4.14 LENS BLOCK CLEANING, 4.14-1

Purpose, 4.14-1
Procedure, 4.14-1
4.15 LASER/FLOW CELL ALIGNMENT, 4.15-1

Purpose, 4.15-1
Flow-Cell Cleaning, 4.15-2
Flow-Cell Z-Axis (Focus) Alignment, 4.15-2
Flow-Cell X- and Y-Axis Plate Alignment, 4.15-4
PN 4235961E xiii
CONTENTS
Flow-Cell Y-Axis Final Alignment, 4.15-6

4.16 ERYTHROLYSE™ II AND STABILYSE™ REAGENT PUMPS ADJUSTMENT AND

REPLACEMENT, 4.16-1
Erythrolyse II and StabiLyse Reagent Pumps Adjustment, 4.16-1
Purpose, 4.16-1
Adjusting the Erythrolyse II Reagent Pumps Volume, 4.16-4
Adjusting the StabiLyse Reagent Pump Volume, 4.16-5
Optimizing the Erythrolyse II Reagent Pumps Volume, 4.16-7
Verifying Erythrolyse II and StabiLyse Reagent Pumps Volume
Adjustment, 4.16-8
Erythrolyse II Reagent Pumps Replacement, 4.16-8
Erythrolyse II Reagent Pumps Removal, 4.16-8
Erythrolyse II Reagent Pump Installation, 4.16-8
4.17 ASPIRATION PUMP VOLUME ADJUSTMENT, 4.17-1

Procedure, 4.17-1
4.18 CBC LYTIC REAGENT PUMPS VERIFICATION, 4.18-1

Procedure, 4.18-1
4.19 RBC/WBC DILUENT DISPENSERS VOLUME TESTING, 4.19-1

Purpose, 4.19-1
Measuring Diluent by Volume, 4.19-1
Procedure, 4.19-1
Measuring Diluent by Weight, 4.19-2
Procedure, 4.19-2
4.20 LYTIC REAGENT AND DILUENT DELIVERY TIMING CHECK, 4.20-1

Purpose, 4.20-1
Procedure, 4.20-1
4.21 BLOOD/BUBBLE DETECTORS GAIN ADJUSTMENT, 4.21-1

Procedure, 4.21-1
4.22 ELECTRONIC POWER SUPPLY REPLACEMENT, 4.22-1

Removal, 4.22-1
Installation, 4.22-1
xiv PN 4235961E
CONTENTS
4.23 VOLTAGE CHECKS AND ADJUSTMENTS, 4.23-1

System Voltage Checks, 4.23-1
Using the DMS to Check System Voltages, 4.23-1
Using an External DMM to Check and Adjust System Voltages, 4.23-1
Laser On Current/LS Offset Voltage Check, 4.23-3
Procedure, 4.23-3
System Voltage Checks Verification, 4.23-4
4.24 PNEUMATIC POWER SUPPLY REPLACEMENT, 4.24-1

Removal, 4.24-1
Setup of Pneumatic Power Supply Buck-Boost Transformer, 4.24-1
4.25 PRESSURE/VACUUM ADJUSTMENT, 4.25-1

General, 4.25-1
System Test (Pressure/Vacuum Verification), 4.25-1
Pressure Adjustments, 4.25-1
60-psi Pressure Adjustment, 4.25-1
Initial Sample Pressure Adjustment, 4.25-5
Vacuum Adjustments, 4.25-5
High Vacuum Verification, 4.25-5
Low Vacuum Adjustment, 4.25-6
4.26 SOLENOID VALVES INSPECTION AND REPLACEMENT, 4.26-1

Solenoid Inspection, 4.26-1
Solenoid Replacement, 4.26-6
Removal, 4.26-6
4.27 HEMOGLOBIN PREAMP MODULE REPLACEMENT, 4.27-1

Purpose, 4.27-1
Removal, 4.27-1
Hemoglobin Lamp Adjustment, 4.27-2
4.28 APERTURE MODULE AND BATH ASSEMBLY, 4.28-1

Removal, 4.28-1
4.29 BSV AND BSV HOUSING/ACTUATOR DISASSEMBLY/REPLACEMENT, 4.29-1

Purpose, 4.29-1
BSV Disassembly/Replacement, 4.29-1
Purpose, 4.29-1
PN 4235961E xv
CONTENTS

BSV Removal, 4.29-1
BSV Installation, 4.29-3
BSV Disassembly/Replacement Verification, 4.29-4
BSV Housing Replacement, 4.29-5
Purpose, 4.29-5
BSV Housing Removal, 4.29-5
BSV Housing Installation, 4.29-5
BSV Actuator Replacement, 4.29-6
Purpose, 4.29-6
BSV Actuator Removal, 4.29-6
BSV Actuator Installation, 4.29-7
BSV Alignment, 4.29-9
BSV and BSV Housing Verification, 4.29-11
Purpose, 4.29-11
Procedure, 4.29-11
4.30 CAROUSEL MOTOR REPLACEMENT, 4.30-1

Removal, 4.30-1
4.31 CODE WHEEL ALIGNMENT, 4.31-1

Procedure, 4.31-1
4.32 NEEDLE REPLACEMENT, 4.32-1

Needle Replacement - MAXM Analyzer with Autoloader Module, 4.32-1
Purpose, 4.32-1
Procedure, 4.32-1
Needle Replacement - MAXM Analyzer with Rotary Cap-Pierce Module, 4.32-5
Purpose, 4.32-5
Procedure, 4.32-5
4.33 RINSE BLOCK ADJUSTMENT, 4.33-1

Procedure, 4.33-1
4.34 AUTOLOADER MODULE REMOVAL, 4.34-1

Purpose, 4.34-1
Removal, 4.34-1
xvi PN 4235961E
CONTENTS
4.35 ROCKER BED ASSEMBLY REMOVAL, 4.35-1

Purpose, 4.35-1
Removal, 4.35-1
4.36 ELEVATOR PLATFORM STEPPER MOTOR REPLACEMENT, 4.36-1

Purpose, 4.36-1
Removal, 4.36-1
4.37 TUBE AVAILABLE SENSOR ASSEMBLY REPLACEMENT, 4.37-1

Purpose, 4.37-1
Removal, 4.37-1
4.38 BAR-CODE READER DECODER CARD CONFIGURATION, 4.38-1

Purpose, 4.38-1
Procedure, 4.38-1
Connecting the Bar-Code Communications Cable, 4.38-1
Selecting the Settings for the Microscan™ Bar-Code Reader Decoder
Card, 4.38-2
Reassembling the Instrument for Operation, 4.38-4
4.39 BAR-CODE SCANNER ALIGNMENT (FOR AUTOLOADER MODULE

ONLY), 4.39-1
Purpose, 4.39-1
Procedure, 4.39-1
Aligning the Bar-Code Scanner, 4.39-2
4.40 ROCKER BED LINKAGE ADJUSTMENT, 4.40-1

General, 4.40-1
Procedure, 4.40-2
4.41 SPECIMEN TUBE STOPPER PIERCE-PROXIMITY ADJUSTMENT, 4.41-1

General, 4.41-1
Purpose, 4.41-1
PN 4235961E xvii
CONTENTS
Preliminary Procedure, 4.41-2

Horizontal Pierce-Proximity Adjustments, 4.41-2
Vertical Pierce-Proximity Adjustments, 4.41-2
4.42 CASSETTE INDEX MOTOR/INDEX HUB GAP ADJUSTMENT, 4.42-1

Purpose, 4.42-1
Procedure, 4.42-1
4.43 SWITCH/SENSOR CHECK, 4.43-1

Purpose, 4.43-1
Procedure, 4.43-1
4.44 NEEDLE-POSITION SENSORS ADJUSTMENT, 4.44-1

Purpose, 4.44-1
Preliminary Check, 4.44-1
Adjustment, 4.44-2
5 MAINTENANCE PROCEDURES, 5.1-1
5.1 MAINTENANCE AND SYSTEM VERIFICATION RECOMMENDATIONS, 5.1-1

Required Service Forms, 5.1-1
Maintenance Schedule, 5.1-1
Verifying Instrument Performance, 5.1-1
Purpose, 5.1-1
Procedure, 5.1-1
Recording Reference Voltages, 5.1-1
6 SCHEMATICS, 6.1-1
6.1 ENGINEERING SCHEMATICS, 6.1-1

Schematics Included, 6.1-1
Schematics Not Included but Available, 6.1-1
7 TROUBLESHOOTING, 7.1-1
7.1 ERROR MESSAGES, 7.1-1
7.2 FLOW-CELL ERRORS FC, PC1, AND PC2, 7.2-1
7.3 DC, RF, OR LS NOISE PROBLEMS, 7.3-1

Purpose, 7.3-1
DC Noise Troubleshooting Checks, 7.3-1
RF Noise Troubleshooting Checks, 7.3-2
LS Noise Troubleshooting Checks, 7.3-7
xviii PN 4235961E
CONTENTS
7.4 DC, RF, OR LS LATEX CALIBRATION PROBLEMS, 7.4-1
7.5 REPORTED INSTRUMENT PROBLEMS AND SOLUTIONS, 7.5-1
8 PARTS LISTS, 8.1-1
8.1 MASTER PARTS LIST, 8.1-1
8.2 ILLUSTRATED PARTS, 8.2-1
A QUICK REFERENCE INFORMATION, A.1-1
A.1 TOLERANCES AND LIMITS, A.1-1

Calibration Tolerances, A.1-1
Electronic Noise Limits, A.1-2
Performance Specifications, A.1-2
Background Counts, A.1-2
Carryover Limits, A.1-2
Reproducibility/Precision Limits, A.1-3
Secondary Mode- to- Primary Mode Comparison, A.1-4
Pneumatic Tolerances, A.1-5
Power Input Specifications and Typical Consumption, A.1-5
Input Power, A.1-5
Input Line Interference, A.1-5
Power Consumption, A.1-6
Timing, A.1-7
VCS Optimization Limits, A.1-8
Voltages, A.1-9
Sample Handler Cards, A.1-9
System, A.1-9
Volumes, A.1-10
Aspiration Pump, Primary Mode, A.1-10
Diluent Dispensers, A.1-10
CBC Lytic Reagent Pumps’ Volume Tolerance, A.1-10
A.2 CIRCUIT CARDS - JUMPERS AND TEST POINTS, A.2-1

376 CPU Card, A.2-1
Jumper Settings, A.2-1
Autoloader Interface Card, A.2-2
Connectors, A.2-2
DILUTER INTERFACE Card, A.2-3
Jumper Setting, A.2-3
Test Point, A.2-3
Fluid Detector/Ram Pressure Card, A.2-4
Connectors, A.2-4
LEDs, A.2-4
I/O Card, A.2-5
Peltier Controller Card, A.2-6
Connectors and LEDs, A.2-6
PN 4235961E xix
CONTENTS
Pneumatic Monitor Card, A.2-7

Connector, A.2-7
Test Points, A.2-8
RAM Timer Card, A.2-9
Rocker Bed Interface Card, A.2-10
Connectors, A.2-10
R/W/P PROC Card, A.2-11
Test Points, A.2-11
Blood/Bubble Detector Adjustments and Test Points - Sample Handler I
Card, A.2-12
Connectors - Sample Handler I Card, A.2-12
Jumper Settings - Sample Handler I Card, A.2-13
LEDs - Sample Handler I Card, A.2-13
Blood/Bubble Detector Adjustments and Test Points- Sample Handler II
Card, A.2-14
Connectors - Sample Handler II Card, A.2-14
Jumper Settings- Sample Handler II Card, A.2-14
LEDs- Sample Handler II Card, A.2-15
VCS PROCESSOR Card, A.2-16
Test Points, A.2-16
A.3 POWER SUPPLIES - SWITCH SETTINGS; FUSE RATINGS; AND

CONNECTIONS, JUMPERS, AND TEST POINTS, A.3-1
Power Switch Settings, A.3-1
Fuse Ratings and Functions, A.3-1
Electronic Power Supply, A.3-2
Jumpers and Connections, A.3-2
Test Points and Adjustments, A.3-2
Laser Power Supply, A.3-4
Pneumatic Power Supply, A.3-4
A.4 SOFTWARE SETTINGS, A.4-1

Bar-Code Reader Decoder Card, A.4-1
A.5 DILUTER COMPONENT LOCATIONS AND FUNCTIONS, A.5-1
A.6 SOFTWARE MENU TREES, A.6-1
B DMS DATABASE FIELDS, B.1-1
B.1 FIELDS CONTAINED IN THE MAXM ANALYZER DATABASE, B.1-1

About the List, B.1-1
MAXM Analyzer 8D Database Fields, B.1-1
xx PN 4235961E
CONTENTS
ABBREVIATIONS, ABBREVIATIONS-1
INDEX, INDEX-1
TRADEMARKS
PN 4235961E xxi
CONTENTS
ILLUSTRATIONS
1.2-1 Triple Transducer Module Laser Warning and Certification Labels, 1.2-2
1.2-2 Bar-Code Reader Laser Warning Labels, 1.2-3
2.1-1 Main Unit with Rotary Cap-Pierce Module, Front View, Main Component
Locations, 2.1-2
2.1-2 Main Unit with Autoloader Module, Front View, Main Component
Locations, 2.1-2
2.1-3 Main Unit Rear View, Main Component Locations, 2.1-3
2.1-4 Main Unit Right-Side View, Main Component Locations, 2.1-3
2.1-5 Main Unit Left-Side View, Main Component Locations, 2.1-4
2.2-1 Electronic Power Supply, Front View, 2.2-2
2.2-2 Electronic Power Supply Components, Top View, Cover Removed, 2.2-3
2.3-1 Signal Flow Block Diagram (MAXM Analyzer with Rotary Cap-Pierce
Module), 2.3-1
2.3-2 Signal Flow Block Diagram (MAXM Analyzer with Autoloader Module), 2.3-2
2.4-1 Circuit Card Locations in the Card Cage, 2.4-1
2.4-2 Initial DMS to Instrument Download, 2.4-2
2.4-3 Subsequent DMS to Instrument Download, 2.4-3
2.4-4 DILUTER INTERFACE Card and Solenoid Junction Card Block Diagram, 2.4-5
2.4-5 I/O Card Block Diagram, 2.4-6
2.4-6 VCS PROCESSOR Card Block Diagram, 2.4-8
2.4-7 R/W/P PROC Card and R/W PREAMP Card Block Diagram, 2.4-11
2.5-1 Pneumatic Power Supply Components, Left-Side View, Compressor Sub-Panel
Removed, 2.5-1
2.5-2 Pressure and Vacuum Air Tank Locations, 2.5-2
2.5-3 Pneumatic Monitor Card Block Diagram, 2.5-4
2.5-4 Monitoring Sequence During Compressor Bleed, 2.5-5
2.6-1 Peltier Controller Card, 2.6-3
2.7-1 Sample Handler Card with Autoloader Module Block Diagram, 2.7-2
2.7-2 Sample Handler Card with Rotary Cap-Pierce Module Block Diagram, 2.7-3
2.8-1 Rotary Cap-Pierce Module Components, 2.8-2
2.9-1 Autoloader Module Components, Front View, Rocker Bed Forward, 2.9-3
2.9-2 Autoloader Module, Front View, Rocker Bed Backward, 2.9-4
2.9-3 Autoloader Module Components, Rear View, 2.9-4
2.10-1 Autosensor Test Card - LEDs Lit Following Power Up, 2.10-1
2.11-1 Solenoid and Tubing Label, 2.11-2
2.11-2 BSV Ports and Wire Markers, 2.11-3
2.11-3 BSV Flow Paths and Wire Markers, 2.11-3
2.11-4 Primary-Mode Aspiration, 2.11-5
2.11-5 WBC/RBC Sample Delivery, 2.11-5
2.11-6 Diff Segmentation, 2.11-6
2.11-7 Diff Segment and Erythrolyse II Reagent Delivery to Mixing Chamber, 2.11-6
2.11-8 Cross Rinse to the Baths, 2.11-7
2.11-9 Secondary-Mode Aspiration, 2.11-8
2.11-10Pre-Prep Latex Aspiration, 2.11-9
2.11-11Sample Delivery, Secondary-Mode, 2.11-9
2.11-12Backwash Functions, 2.11-10
2.12-1 Applying the Coulter Principle, 2.12-1
2.13-1 Applying the VCS Technology, 2.13-3
xxii PN 4235961E
CONTENTS
2.13-2 Light Scatter Path, 2.13-3

2.13-3 Hydrodynamically Focused Cells, 2.13-4
2.13-4 Flow Cell Hydraulics, 2.13-4
2.13-5 RF Detector Preamp Card Signal Flow, 2.13-6
2.13-6 LS Preamp 5 Module Block Diagram, 2.13-7
2.13-7 LS Sensor Regions, 2.13-7
3.2-1 MAXM Analyzer with Autoloader Module Main Component Layout, 3.2-1
3.2-2 TTM Baseplate Shipping Screws, 3.2-2
3.2-3 Compressor Shipping Pins, 3.2-3
3.2-4 Rocker Bed Lock-Down Screw, 3.2-5
3.3-1 Electronic Cable Connections, 3.3-1
3.3-2 Bar-Code Wand Connections, 3.3-2
3.3-3 DMS Line Voltage Select Switch, 3.3-3
3.3-4 Reagent and Drain Connections, 3.3-4
3.10-1 Reconfiguring the Instrument for the Cyanide-Free Reagent System, 3.10-2
4.1-1 Switches for Clearing the CPU RAM, 4.1-3
4.2-1 Control and Sample Files Available for Viewing, 4.2-4
4.2-2 Single-Parameter Graph, 4.2-5
4.2-3 Parameter vs. Parameter Graph, 4.2-6
4.2-4 Multiple-Parameter Graph, 4.2-6
4.2-5 Flag Graph, 4.2-6
4.2-6 Statistics Window, 4.2-7
4.2-7 Sequential (Points) Mode, 4.2-7
4.2-8 Zoom Mode, 4.2-7
4.2-9 Histogram Mode, 4.2-8
4.2-10 Date-Offset Mode, 4.2-8
4.2-11 Control-File Graph, 4.2-8
4.2-12 Service Disk Utilities Menu Tree, 4.2-9
4.9-1 Optimization Procedure Flow, 4.9-1
4.9-2 Histogram of Good Count Ratio Data, 4.9-3
4.9-3 Histogram of Poor Count Ratio Data, 4.9-3
4.10-1 Unacceptable Flow-Rate Graph, 4.10-2
4.13-1 Wetting Applicator with Alcohol, 4.13-2
4.13-2 Wiping Front of Flow Cell, 4.13-2
4.13-3 Wiping Rear of Flow Cell, 4.13-2
4.15-1 Laser/Flow-Cell Alignment Work Flow, 4.15-1
4.15-2 Flow-Cell Z-Axis Alignment, 4.15-3
4.15-3 Scatter Sensor Laser Diffraction, 4.15-4
4.15-4 Flow-Cell X- and Y-Axis Plate Alignment, 4.15-5
4.15-5 Laser Reflected Light Beam with Special Light Shield Installed, 4.15-6
4.15-6 Laser Diffraction Pattern on Paper, 4.15-7
4.16-1 Match Sheath Fluid to Sample Stream Conductivity, 4.16-1
4.16-2 Effect of Dilution Conductivity on Count Ratio, 4.16-2
4.16-3 Effect of Osmolality on NE DC mean, 4.16-2
4.16-4 Erythrolyse II Reagent and StabiLyse Reagent Pump Optimization Flow
Diagram, 4.16-3
4.16-5 Tubing from Mixing Chamber, 4.16-4
4.16-6 Volumetric Cylinder Calibration Marks, 4.16-4
4.16-7 Reading the Meniscus, 4.16-5
4.16-8 Diaphragm Pump Adjustment, 4.16-5
PN 4235961E xxiii
CONTENTS
4.16-9 Pump Module Mounting Bracket, Old Configuration, 4.16-8

4.16-10Pump Module Mounting Bracket, New Configuration, 4.16-8
4.17-1 Second Pull (Vacuum), 4.17-1
4.17-2 First Pull (Aspiration Pump)), 4.17-2
4.19-1 Reading a Meniscus, 4.19-2
4.20-1 WBC Bath Tubing Disconnect for Lytic Reagent/Diluent Delivery Timing
Hookup, 4.20-2
4.20-2 Test Box and Transducer Hookup for Lytic Reagent/Diluent Timing
Procedure, 4.20-2
4.20-3 Digital Pressure Meter Hookup for Lytic Reagent/Diluent Timing
Procedure, 4.20-3
4.21-1 Blood/Bubble Detector Test Points and Adjustments, 4.21-1
4.22-1 Electronic Power Supply Connectors, 4.22-1
4.23-2 Electronic Power Supply, Top View with Cover Removed, 4.23-2
4.24-1 Pneumatic Supply Removal, 4.24-1
4.25-1 60-psi Pressure Regulator, Old Configuration, 4.25-2
4.25-2 60-psi Pressure Regulator, New Configuration, 4.25-2
4.27-1 Hgb Preamp Module, 4.27-1
4.28-1 Aperture Module and Bath Assembly, 4.28-1
4.29-1 Retaining the Probe-Slide Mechanism, 4.29-1
4.29-2 Removing the Rinse Block Screw and Washer, 4.29-2
4.29-3 Moving the Air Cylinder, 4.29-2
4.29-4 Aligning the Air Cylinder, 4.29-3
4.29-5 Replacing the Rinse Block, 4.29-4
4.29-6 BSV Actuator (Configuration A) Allen Screw and Cylinder Seals, 4.29-6
4.29-7 BSV Actuator Removal, Configuration A, 4.29-7
4.29-8 BSV Actuator Removal, Configuration B, 4.29-7
4.29-9 Aligning the BSV Housing, 4.29-9
4.29-10Aspiration First Pull (Aspiration Pump), 4.29-11
4.29-11Aspiration Second Pull (Vacuum), 4.29-12
4.30-1 Carousel Motor and Carousel Assembly, 4.30-1
4.31-1 Code Wheel, 4.31-2
4.32-1 Cleaning Solution, 4.32-1
4.32-2 Cassette into Loading Bay, 4.32-1
4.32-3 Safety Clip Installation, 4.32-2
4.32-4 Needle Cartridge Removal, 4.32-2
4.32-5 Needle Assembly Location Grooves, 4.32-3
4.32-6 Needle Assembly Tubing, 4.32-3
4.32-7 Needle Tubing Quick Disconnects, 4.32-4
4.32-8 Needle Assembly Installation, 4.32-4
4.32-9 Safety Clip Removal, 4.32-4
4.32-10Cleaning Solution, 4.32-5
4.32-11Exit Tray Removal, 4.32-6
4.32-12Needle Tubing Quick Disconnects, 4.32-6
4.32-13LOCK Lever, 4.32-6
4.32-14Needle Assembly Removal, 4.32-7
4.32-15Needle Assembly Orientation, 4.32-7
4.32-16Needle Assembly Alignment, 4.32-7
xxiv PN 4235961E
CONTENTS
4.32-17Slots, 4.32-8
4.32-18Needle Assembly Installation, 4.32-8
4.32-19Needle Assembly Quick Disconnects, 4.32-8
4.32-20Exit Tray Reinstallation, 4.32-9
4.33-1 Rinse Block Adjusting Screw, Old BSV Configuration, 4.33-1
4.33-2 Rinse Block Adjusting Screw, New BSV Configuration, 4.33-2
4.34-1 Autoloader Module Guide Rails, 4.34-2
4.34-2 Autoloader Module Removal, 4.34-2
4.35-1 Rocker Bed Removal, 4.35-1
4.35-2 Rocker Bed Hardware, 4.35-2
4.39-1 Bar-Code Scanner Adjustment Screws, 4.39-2
4.40-1 Rocker Bed Linkage, 4.40-1
4.40-2 Rocker Bed Linkage Adjustment, 4.40-2
4.41-1 Specimen Tube Stopper Piercing Proximity, 4.41-1
4.42-1 Index Motor/Index Hub Gap Adjustment, 4.42-1
4.44-1 Needle-Position Sensor Orientation on the Needle Drive Cylinder, 4.44-1
7.3-1 Acceptable DC Noise Display, 7.3-1
7.3-2 Unacceptable DC Noise Display, 7.3-1
7.3-3 Acceptable Static RF Noise Display, 7.3-2
7.3-4 Unacceptable Static RF Noise Display, 7.3-2
7.3-5 Acceptable Dynamic RF Noise, 7.3-3
7.3-6 RF 60 Hz, 7.3-4
7.3-7 RF 60 Hz with Noise Spikes, 7.3-4
7.3-8 Normal RF Random Baseline Bounce, 7.3-4
7.3-9 RF Output with bubble Trapped in Sheath 2, 7.3-5
7.3-10 Normal RF Baseline While Cycling Diluent, 7.3-5
7.3-11 Noisy RF Baseline While Cycling Diluent, 7.3-5
7.3-12 Noisy RF Baseline While Cycling Diluent (RF Noise >100 mV), 7.3-6
7.3-13 LATRON Control Sample with a Good RF Tube and Box, 7.3-6
7.3-14 LATRON Control Sample with a Bad RF Tube and Box, 7.3-6
7.3-15 RF Output Running LATRON Control (Leak at QD7), 7.3-7
7.3-16 LATRON Control Run with DC Gain Drop, 7.3-7
7.3-17 Acceptable Static LS Noise Display, 7.3-8
7.3-18 Unacceptable Static LS Noise Display, 7.3-8
7.3-19 Acceptable Dynamic LS Noise Display, 7.3-9
8.2-1 Main MAXM Analyzer Components Illustrated, 8.2-1
8.2-2 CBC Module, Front View (See Table 8.2-2), 8.2-2
8.2-3 CBC Module, Rear View (See Table 8.2-3), 8.2-4
8.2-4 Mixing Module, Front View (See Table 8.2-4), 8.2-6
8.2-5 Mixing Module, Right Side View (See Table 8.2-5), 8.2-8
8.2-6 Mixing Module, Left Side View (See Table 8.2-6), 8.2-10
8.2-7 BSV Module with BSV that Uses Air Cylinders, Front View
(See Table 8.2-7), 8.2-12
8.2-8 BSV Module with BSV that Uses an Actuator, Front View (See Table 8.2-8), 8.2-14
8.2-9 BSV Module, Rear View (See Table 8.2-9), 8.2-16
8.2-10 BSV Module, Ball and Slide Assembly (See Table 8.2-10), 8.2-18
8.2-11 Pump Module, Old Configuration (See Table 8.2-11), 8.2-20
8.2-12 Pump Module, New Configuration (See Table 8.2-11), 8.2-22
8.2-13 Rotary Cap-Pierce Module (See Table 8.2-13), 8.2-24
8.2-14 Rotary Cap-Pierce Module, Carousel Assembly (See Table 8.2-14), 8.2-26
PN 4235961E xxv
CONTENTS
8.2-15 Autoloader Module, Front View (See Table 8.2-15), 8.2-28

8.2-16 Autoloader, Rear View (See Table 8.2-16), 8.2-30
8.2-17 Autoloader, Rocker Bed Hardware and Sensors (See Table 8.2-17), 8.2-32
8.2-18 Needle Assembly and Sensors (See Table 8.2-18), 8.2-34
8.2-19 Triple Transducer Module (See Table 8.2-19), 8.2-36
8.2-20 Flow Cell and Laser Assembly (See Table 8.2-20), 8.2-38
8.2-21 Main Diluter Module (See Table 8.2-21), 8.2-40
8.2-22 Electronic Power Supply, Top View (See Table 8.2-22), 8.2-42
8.2-23 Pneumatic Power Supply, Left-Side View (See Table 8.2-23), 8.2-44
8.2-24 Analyzer Module, Card Cage Components (See Table 8.2-24), 8.2-46
8.2-25 Wire Harness Components (See Table 8.2-25), 8.2-48
8.2-26 Electrical Connectors, 8.2-50
A.1-1 Diluent and Lyse Timing Cycle, A.1-7
A.2-1 376 CPU Card Jumper Locations, A.2-1
A.2-2 Autoloader Interface Component Locations, A.2-2
A.2-3 DILUTER INTERFACE Card Component Locations, A.2-3
A.2-4 Fluid Detector/Ram Pressure Card Component Locations, A.2-4
A.2-5 I/O Card Jumper Locations, A.2-5
A.2-6 Peltier Controller Card Connector and LED Locations, A.2-6
A.2-7 Pneumatic Monitor Card Component Locations, A.2-7
A.2-8 RAM Timer Card Jumper Locations, A.2-9
A.2-9 Rocker Bed Interface Card Connector Locations, A.2-10
A.2-10 R/W/P PROC Card Component Locations, A.2-11
A.2-11 Sample Handler I Card Component Locations, A.2-12
A.2-12 Sample Handler II Card Component Locations, A.2-14
A.2-13 VCS PROCESSOR Card Component Locations, A.2-16
A.5-1 BSV Module, Old Configuration, Front View, A.5-7
A.5-2 BSV Module, New Configuration, Front View, A.5-7
A.5-3 BSV Module, Rear View, A.5-8
A.5-4 CBC Module, Front View, A.5-8
A.5-5 CBC Module, Rear View, A.5-9
A.5-6 Main Diluter Module, Front View, A.5-9
A.5-7 Main Diluter Module, Right-Side View, A.5-10
A.5-8 Mixing Module, Front View, A.5-10
A.5-9 Mixing Module, Rear View, A.5-11
A.5-10 Pump Module, Old Configuration, A.5-11
A.5-11 Pump Module, New Configuration, A.5-12
A.6-1 MAXM Analyzer with Rotary Cap-Pierce Module Software Menu Tree, Revision
8D and Higher, A.6-1
A.6-2 MAXM Analyzer with Autoloader Module Software Menu Tree, Revision 8D and
Higher, A.6-2
A.6-3 Service Disk Software Utility Menu Tree, A.6-3
xxvi PN 4235961E
CONTENTS
TABLES
2.6-1 Fluid Detector/Ram Pressure Card LED Positions (Viewing from the Rear of the
Unit), 2.6-2
2.6-2 Ambient Versus Erythrolyse II Reagent Temperatures, 2.6-4
2.6-3 Temperature Range Cycles, 2.6-5
2.8-1 Rotary Cap-Pierce Module - Motors, Solenoids, and Sensors/Switches, 2.8-1
2.9-1 Autoloader Module - Motors, Solenoids, and Sensors/ Switches, 2.9-1
3.1-1 Fan and Vent Locations, 3.1-2
3.2-1 Deactivator Clip Locations, 3.2-2
4.2-1 DMS Service Menu Options, 4.2-2
4.2-2 Service Disk Utilities Selections/Explanations, 4.2-10
4.9-1 Purpose of VCS Optimization Procedures, 4.9-2
4.26-1 Solenoid Operations, 4.26-1
4.38-1 Moving Around the Microscan Screens, 4.38-3
4.43-1 Switch/Sensor Checks on the MAXM Analyzer with Autoloader Module, 4.43-1
4.43-2 Switch/Sensor Checks on the MAXM Analyzer with Rotary Cap-Pierce
Module, 4.43-5
6.1-1 Schematics Included in This Manual, 6.1-1
6.1-2 Schematics Available for the MAXM Analyzer but Not Included in This
Manual, 6.1-1
7.1-1 System Error Messages - Symbols and Numbers, 7.1-1
7.1-2 System Error Messages - A and B, 7.1-5
7.1-3 System Error Messages - C, 7.1-7
7.1-4 System Error Messages - D through H, 7.1-13
7.1-5 System Error Messages - I, 7.1-16
7.1-6 System Error Messages - J through P, 7.1-19
7.1-7 System Error Messages - R, 7.1-22
7.1-8 System Error Messages - S, 7.1-24
7.1-9 System Error Messages - T, 7.1-27
7.1-10 System Error Messages - U, 7.1-28
7.1-11 System Error Messages - V through Z, 7.1-30
7.1-12 Internal Instrument Codes, 7.1-32
7.2-1 Flow-Cell Errors, 7.2-1
7.3-1 RF Troubleshooting Table, 7.3-3
7.4-1 RF, DC, or LS Latex Calibration Troubleshooting Table, 7.4-1
7.5-1 Troubleshooting Reported Instrument Problems, 7.5-1
8.1-1 Part Categories, 8.1-1
8.1-2 Accessories, 8.1-1
8.1-3 Autoloader Module, 8.1-2
8.1-4 Bar-Code System, 8.1-4
8.1-5 BSV Module, 8.1-5
8.1-6 CBC Module, 8.1-7
8.1-7 Circuit Cards, 8.1-8
8.1-8 Covers/Doors, 8.1-9
8.1-9 Fuses, 8.1-9
8.1-10 Interconnect Cables, 8.1-10
8.1-11 Main Diluter Module, 8.1-11
8.1-12 Miscellaneous Hardware, 8.1-12
8.1-13 Mixing Module, 8.1-15
PN 4235961E xxvii
CONTENTS
8.1-14 Options/Upgrade Kits, 8.1-16

8.1-15 Pickup Tubes and Sensors, 8.1-16
8.1-16 Power Supplies, 8.1-17
8.1-17 Pump Module, 8.1-18
8.1-18 Rotary Cap-Pierce Module, 8.1-19
8.1-19 Shielded Reagent Lines, 8.1-21
8.1-20 Software, 8.1-21
8.1-21 Tools and Supplies, 8.1-22
8.1-22 Triple Transducer Module, 8.1-23
8.1-23 Tubing, 8.1-25
8.2-1 Illustrations Not Referenced from Figure 8.2-1, 8.2-1
8.2-2 CBC Module, Front View (See Figure 8.2-2), 8.2-3
8.2-3 CBC Module, Rear View (See Figure 8.2-3), 8.2-5
8.2-4 Mixing Module, Front View (See Figure 8.2-4), 8.2-7
8.2-5 Mixing Module, Right Side View (See Figure 8.2-5), 8.2-9
8.2-6 Mixing Module, Left Side View (See Figure 8.2-6), 8.2-11
8.2-7 BSV Module with BSV that Uses Air Cylinders, Front View (See
Figure 8.2-7), 8.2-13
8.2-8 BSV Module with BSV that Uses an Actuator, Front View (See
Figure 8.2-8), 8.2-15
8.2-9 BSV Module, Rear View (See Figure 8.2-9), 8.2-17
8.2-10 BSV Module, Ball and Slide Assembly (See Figure 8.2-10), 8.2-19
8.2-11 Pump Module, Old Configuration (See Figure 8.2-11), 8.2-21
8.2-12 Pump Module, New Configuration (See Figure 8.2-12), 8.2-23
8.2-13 Rotary Cap-Pierce Module (See Figure 8.2-13), 8.2-25
8.2-14 Rotary Cap-Pierce Module, Carousel Assembly (See Figure 8.2-14), 8.2-27
8.2-15 Autoloader Module, Front View (See Figure 8.2-15), 8.2-29
8.2-16 Autoloader, Rear View (See Figure 8.2-16), 8.2-31
8.2-17 Autoloader Module, Rocker Bed Sensors and Hardware (See
Figure 8.2-17), 8.2-33
8.2-18 Autoloader Module Needle Assembly and Sensors (See Figure 8.2-18), 8.2-35
8.2-19 Triple Transducer Module (See Figure 8.2-19), 8.2-37
8.2-20 Flow-Cell and Laser Assembly (See Figure 8.2-20), 8.2-39
8.2-21 Main Diluter Module (See Figure 8.2-21), 8.2-41
8.2-22 Electronic Power Supply, Top View (See Figure 8.2-22), 8.2-43
8.2-23 Pneumatic Power Supply, Left-Side View (See Figure 8.2-23), 8.2-45
8.2-24 Analyzer Module, Card Cage Components (See Figure 8.2-24), 8.2-47
8.2-25 Wire Harness Components (See Figure 8.2-25), 8.2-49
A.1-1 Acceptable Cal Factor Ranges, A.1-1
A.1-2 Acceptable CV Limits for Initial Adjustments to 5C Cell Control, A.1-1
A.1-3 CBC Latex Calibration, A.1-1
A.1-4 RMS Noise Checks - CBC, A.1-2
A.1-5 Primary/Secondary-Mode Background Counts, A.1-2
A.1-6 Primary/Secondary-Mode Carryover Limits, A.1-2
A.1-7 Retic Mode-to-Mode and Within-Mode Carryover Limits*, A.1-2
A.1-8 Acceptable Blood Parameter Limits for Reproducibility Specimen, A.1-3
A.1-9 Acceptable Manual Diff Limits for Reproducibility Specimen, A.1-3
A.1-10 CBC and Diff Reproducibility Limits, A.1-4
xxviii PN 4235961E
CONTENTS
A.1-11 Retics Reproducibility Limits, A.1-4

A.1-12 Secondary Mode-to-Primary Mode Comparison Limits, A.1-4
A.1-13 Pressure and Vacuum Tolerances, A.1-5
A.1-14 Input Line Interference Limits, A.1-5
A.1-15 Typical Power Consumption at 120 Vac, A.1-6
A.1-16 Diluent and Lyse Timing Acceptable Choke Combinations, A.1-7
A.1-17 LATRON™ Primer Background Limits, A.1-8
A.1-18 LATRON™ Control Calibration and Verification Limits, A.1-8
A.1-19 LS Offset Voltage/Laser On Current Checks, A.1-8
A.1-20 Noise Checks - Diff and Retic, A.1-8
A.1-21 Hgb Blank Voltage, A.1-9
A.1-22 Blood/Bubble Detectors Tolerances, A.1-9
A.1-23 System Voltage Ranges, A.1-9
A.1-24 Aspiration Pump Volume Tolerances, A.1-10
A.1-25 Diluent Dispenser Volume Specifications and Tolerances, A.1-10
A.2-1 376 CPU Card Jumper Settings (See Figure A.2-1), A.2-1
A.2-2 Autoloader Interface Card Connectors (See Figure A.2-2), A.2-2
A.2-3 Fluid Detector/Ram Pressure Card Connectors (See Figure A.2-4), A.2-4
A.2-4 Fluid Detector/Ram Pressure Card LED Positions (See Figure A.2-4), A.2-4
A.2-5 Peltier Controller Card Connectors and LEDs (See Figure A.2-6), A.2-6
A.2-6 Pneumatic Monitor Card Pneumatic Reading to Vdc Conversion (See
Figure A.2-7), A.2-8
A.2-7 Rocker Bed Interface Card Connectors (See Figure A.2-9), A.2-10
A.2-8 R/W/P PROC Card Jumper Settings (See Figure A.2-10), A.2-11
A.2-9 R/W/P PROC Card Test Points (See Figure A.2-10), A.2-11
A.2-10 Sample Handler I Card Connectors (See Figure A.2-11), A.2-12
A.2-11 Sample Handler I Card Jumper Settings (See Figure A.2-11), A.2-13
A.2-12 Sample Handler I Card LED Functions (See Figure A.2-11), A.2-13
A.2-13 Sample Handler II Card Connectors (See Figure A.2-12), A.2-14
A.2-14 Sample Handler II Card Jumper Settings (See Figure A.2-12), A.2-14
A.2-15 Sample Handler II Card LED Functions (See Figure A.2-12), A.2-15
A.2-16 VCS PROCESSOR Card Test Points (See Figure A.2-13), A.2-16
A.3-1 Input Power Switch Settings, A.3-1
A.3-2 Fuse Ratings and Functions, A.3-1
A.3-3 Electronic Power Supply Terminal Card Jumpers and Connections, A.3-2
A.3-4 Electronic Power Supply Test Points and Adjustments, A.3-2
A.3-5 Laser Power Supply Buck-Boost Transformer Jumpers and Connections, A.3-4
A.3-6 Pneumatic Power Supply Buck-Boost Transformer Terminal Card Jumpers and
Connections, A.3-4
A.4-1 Bar-Code Reader Decoder Card Code Types Default Settings, A.4-1
A.4-2 Bar-Code Reader Decoder Card Communications Default Settings, A.4-1
A.4-3 Bar-Code Reader Decoder Card Operations Default Settings, A.4-2
A.4-4 Bar-Code Reader Decoder Card User Outputs Default Settings, A.4-2
A.5-1 Diluter Component Location References and Functions, A.5-1
PN 4235961E xxix
CONTENTS
xxx PN 4235961E
CONTENTS
1
1 INTRODUCTION, 1.1-1
1.1 MANUAL DESCRIPTION, 1.1-1

Scope, 1.1-1
Intended Audience, 1.1-1
Organization, 1.1-2
Numbering Format, 1.1-2
Special Headings, 1.1-3
WARNING, 1.1-3
CAUTION, 1.1-3
IMPORTANT, 1.1-3
ATTENTION, 1.1-3
Note, 1.1-3
Conventions, 1.1-4
1.2 SAFETY PRECAUTIONS, 1.2-1

Laser, 1.2-1
Laser Beam Hazards, 1.2-1
Laser Radiation Hazards, 1.2-2
Electronic, 1.2-3
Biological, 1.2-3
Troubleshooting, 1.2-4
ILLUSTRATIONS
1.2-1 Triple Transducer Module Laser Warning and Certification Labels, 1.2-2
1.2-2 Bar-Code Reader Laser Warning Labels, 1.2-3
PN 4235961E 1-i
CONTENTS
1-ii PN 4235961E
1INTRODUCTION 1
1.1 MANUAL DESCRIPTION
Scope
This manual provides the reference information and procedures needed for servicing and
maintaining the Main Unit of the COULTER® MAXM™ analyzer and the COULTER MAXM
analyzer with Autoloader., both with and without the reticulocyte analysis option It is
available both online and in hard copy. The online manual is released on the Service Resource
Kit CD-ROM, PN 641747.
This manual is to be used in conjunction with the following COULTER MAXM Analyzer and
COULTER MAXM Analyzer with Autoloader customer documents and does not contain
information and procedures already covered in these documents:
r Operating Cues, PN 4235936

r Operator's Guide, PN 4235935 (PN 4237245 for United Kingdom)
r Reference Manual, PN 4235933 (PN 4237244 for United Kingdom)
r Special Procedures and Troubleshooting, PN 4235934 (PN 4237246 for
United Kingdom).
r Master Index, PN 4237155-6
To service the DMS, refer to the DMS Configuration Listing for STKS™, MAXM™, and HmX
Series Systems, PN 4276614.
Any service memo that affects the information in this manual will include either minor
revision change pages or a Notice of Information Update form for this manual. A Notice of
Information Update form will summarize the changes and will list the specific headings,
figures, and tables affected.
Intended Audience
To use this manual effectively, you need the following:
r An operator’s knowledge of the MAXM hematology analyzer
r Service training on the MAXM hematology analyzer
r A thorough understanding of -
t Basic electronic and pneumatic principles and devices
t Hematology terms and concepts
t The Coulter Principle
t VCS technology
t COULTER Histogram Differential and Interpretive Reporting
t Reagent systems
t Quality control
t Troubleshooting techniques.
r The ability to -
t Read pneumatic/hydraulic schematics and understand related terminology
t Read electronic schematics and understand related terminology
t Use a digital multimeter and an oscilloscope
t Use basic mechanical tools and understand related terminology.
PN 4235961E 1.1-1
INTRODUCTION
MANUAL DESCRIPTION
Organization
The information in this manual is organized into10 chapters. To make it easier to access the
information:
r In the online manual, each page or screen has -

t A Contents button linked to a master table of contents
t An Illustrations button linked to a list of illustrations
t A Tables button linked to a list of tables
t An Index button linked to an alphabetic index.
r In the printed manual, there is a master table of contents (including master lists of the
illustrations and the tables) at the beginning of the manual, a chapter-specific table of
contents at the beginning of each chapter, and an alphabetic index at the end of the
manual.
CHAPTER 1, INTRODUCTION, includes a brief description of this manual, a list of
supporting documents, and essential safety information.
CHAPTER 2, INSTRUMENT DESCRIPTION, includes the function and location of each

major MAXM analyzer component and an explanation of the instrument’s operation.
CHAPTER 3, INSTALLATION PROCEDURES, contains the procedures used to install the

MAXM analyzer.
CHAPTER 4, SERVICE AND REPAIR PROCEDURES, contains the procedures used to

service and repair the MAXM analyzer. When it is applicable, the reason for doing a
procedure and any special tools or equipment needed are listed before the instructions.
CHAPTER 5, MAINTENANCE PROCEDURES, contains the procedures used to maintain

the MAXM analyzer and to verify the system is operating correctly.
CHAPTER 6, SCHEMATICS, is provided for the insertion of MAXM analyzer schematics.
CHAPTER 7, TROUBLESHOOTING, contains problem descriptions, including a complete

list of error messages, with troubleshooting actions and tips. Use the information in this
chapter in conjunction with the procedures in Chapter 4 to locate and correct instrument
problems.
CHAPTER 8, PARTS LISTS, includes a master list of the MAXM analyzer field replaceable
components (FRC) and field replaceable units (FRU) and illustrations of these components.
CHAPTER 9, APPENDICES, is provided for the addition of reference material and includes
Appendix A, QUICK REFERENCE INFORMATION. Appendix A contains instrument
specifications and tolerances; circuit card and power supply jumper and switch settings and
test point values; and software menu trees.
CHAPTER 10 contains a list of ABBREVIATIONS used in this manual.
Numbering Format
Each chapter of this manual is further divided into topics, which are numbered sequentially,
beginning at one. The numbering format for the topic heading, which is called the primary
1.1-2 PN 4235961E
INTRODUCTION
MANUAL DESCRIPTION 1
heading, is chapter number, decimal point, topic number. For example, the primary heading
number for the fifth topic covered in Chapter 2 is 2.5.
The page, figure and table numbers are tied directly to the primary heading number. For
example, Heading 2.5 begins on page 2.5-1, the first figure under Heading 2.5 is Figure 2.5-1,
and the first table under Heading 2.5 is Table 2.5-1.
Note: Primary headings always begin on the top of a right-hand page.
Special Headings
Throughout this manual, WARNING, CAUTION, IMPORTANT, ATTENTION, and Note
headings are used to indicate potentially hazardous situations and important or
helpful information.
WARNING
A WARNING indicates a situation or procedure that, if ignored, can cause serious personal
injury. The word WARNING is in bold-faced text in the printed manual and is red in the
online manual.
CAUTION
A CAUTION indicates a situation or procedure that, if ignored, can cause damage to
equipment. The word CAUTION is in bold-faced text in the printed manual and is red in the
online manual.
IMPORTANT
An IMPORTANT indicates a situation or procedure that, if ignored, can result in erroneous
test results. The word IMPORTANT is in bold-faced text in the printed manual and is red in
the online manual.
ATTENTION
An ATTENTION contains information that is critical for the successful completion of a
procedure and/or operation of the instrument. The word ATTENTION is in bold-faced text in
the printed manual and is red in the online manual.
Note
A Note contains information that is important to remember or helpful in performing
a procedure. The word Note is in bold-faced text.
PN 4235961E 1.1-3
INTRODUCTION
MANUAL DESCRIPTION
Conventions
This manual uses the following conventions to make the material clearer and more concise,
or to enhance access speed in the electronic version of the manual. An example is given below
each explanation.
1. Messages that appear on a screen are in italics.

The instrument processes the request and displays S/A 2 °MODE ON in the System Status
field.
2. Selections that appear on a screen are boldfaced.
Select Waste = OFF if the unit is draining directly into a plumbing system.
3. Information that is to be typed is in Courier font.
Type the password SERVICE, then press Û.
4. Keys on the DMS are boxed.
Press Ò to save data and escape.
5. Keys that are to be pressed simultaneously are printed with a plus sign between the keys.
Press Î then Þ+Î to display the average histograms for both RBC and WBC.
6. The software path to a specific function or screen appears with the double solid-right
triangle ( tt ) symbol between succeeding screen options.
From the Special Functions menu, select Diagnostics tt Operator Options tt Fluidic Test tt
Disable Reagent Sensors.
7. When the word "select" is used in conjunction with the screen and its associated keys,
you are to press the key or keys necessary to display the desired screen or to initiate the
desired function.
Select the Calibration Reproducibility screen (Special Functions tt Calibration tt
Reproducibility).
8. In the electronic version of the manual:
a. Links to additional information are in blue and are underlined.
Activate the service options as directed under Heading 4.2, USING THE SERVICE
DISK.
To access the linked information, select the blue, underlined text.
b. The material is divided into many small sections (electronic files).
r Every primary heading is a separate file and whenever possible the amount of
material contained within one primary heading is limited to four to ten pages.
r If a primary heading must be large, such as the illustrated parts list, invisible
breaks are added to the electronic file to further divide it.
To move from one section (electronic file) to the next in an HTML version of the
manual, use the right and left arrows on the navigation bars displayed at the top and
bottom of each section.
9. Unless otherwise noted, left/right orientation to components is presented as if you are
looking at the front of the instrument.
1.1-4 PN 4235961E
INTRODUCTION
SAFETY PRECAUTIONS 1
1.2 SAFETY PRECAUTIONS
Laser
Laser Beam Hazards
WARNING Risk of personal injury. The laser beam can cause eye damage if viewed either directly or
indirectly from reflective surfaces (such as a mirror or shiny metallic surface). Avoid direct exposure to the
beam. Do not view it directly or with optical instruments except from special service tools as directed in
this manual.
Because the system contains a laser, the customer should keep it isolated from nonlaser
instruments. The customer should also keep a copy of ANSI standard 136.1, SAFE USE OF
LASERS, near the instrument for ready reference. Copies are available from:
American National Standards Institute

1430 Broadway
New York, NY 10018
The laser, located in the Triple Transducer module (TTM), is a unique light source that shows
characteristics different from conventional light sources. The safe use of the laser depends
upon familiarity with the instrument and with the properties of coherent, intense beams
of light.
Eye and skin damage, as well as instrument damage, can be caused by the laser beam. The
laser has enough power to ignite substances placed in the beam path, even at a distance.
Specular reflection results from indirect contact with the laser beam from reflective surfaces,
such as jewelry, and can cause damage. Follow these precautions when working near an
exposed laser:
r Never look directly into the laser light source or at scattered laser light from any
reflective surface.
r It is recommended that you wear proper laser safety glasses when performing service or
maintenance on the system. The glasses help prevent accidental exposure to the output
beam and its reflection.
r Do not use lasers in the presence of flammables or explosives; these include volatile
substances such as alcohol, solvents, and ether.
r Avoid direct exposure and indirect reflection of the laser beam to your skin.
r Assure that any spectators are not potentially exposed to a hazardous condition.
r Do not leave the laser unattended if there is a chance that an unauthorized person may
attempt to use it.
PN 4235961E 1.2-1
INTRODUCTION
SAFETY PRECAUTIONS
Laser Radiation Hazards
WARNING Risk of personal injury from radiation exposure. Performing procedures other than those
specified in this manual could result in hazardous radiation exposure. Do not use any controls, make any
adjustments, or perform any procedures other than those specified in this manual.
In its design and manufacture of the MAXM analyzer, Coulter Corporation has complied with
the requirements governing the use and application of a laser as stipulated in regulatory
documents issued by the U.S. Department of Health and Human Services, and by the Center
for Devices and Radiological Health (CDRH). In compliance with these regulatory
documents, every measure has been taken to ensure the health and safety of users, laboratory
personnel, and service personnel from the possible dangers of laser use. The laser is classified
as Class I when it is in the system with the protective housing in place.
Note: As installed in the Triple Transducer Module (TTM) safety fixture, the laser presents no
radiation hazard to users and the instrument complies with 21 CFR 1040.
CDRH-approved labels are placed near or on those covers that when removed might expose
laser radiation. Figures 1.2-1 and 1.2-2 show the Warning and Certification labels. The
MAXM analyzer is a Class I laser product per BS 60825.
Figure 1.2-1 Triple Transducer Module Laser Warning and Certification Labels
LASER RADIATIONIS
EMITTED FROMTHIS
CAUTION
APERTURE ELECTRIC SHOCK HAZARD.
A LASER RADIATION VOID
DIRECTEYE EXPOSURE
DISCONNECT UNIT FROM
AVOID EXPOSURE POWER SOURCE PRIOR
HELIUM - NEON/4m
CLASS IIIa LASER PRODUCT
TO SERVICING.
LASER RADIATION WHEN

OPEN AND INTERLOCK
DEFEATED
AVOID EYE OR SKIN

EXPOSURE TO DIRECT OR
SCATTERED RADIATION
2413943
LASER RA DIATION W HE N
OP EN AND INTERLOCK
DE FEATED
AVOID EYE OR SKIN

EXPO SU RE TO DIRE CT OR
SCA TTERE D RA DIATION
2413943
1096 Mellon Avenue

Manteca, CA 95337
MODEL
LASER RADIATION WHEN MANUFACTURED
OPEN AND INTERLOCK SERIAL NO.
DEFEATED
THIS LASER DOES NOT
AVOID EYE OR SKIN COMPLY WITH 21 CFR 1040.
EXPOSURE TO DIRECT OR USE ONLY AS A COMPONENT.
SCATTERED RADIATION
SEE INSTALLATION INSTRUCTIONS.
2413943
PATENT NOS MADE
4352,185 4631,727 IN
4750,182 4864,583 USA
5933002D
1.2-2 PN 4235961E
INTRODUCTION
SAFETY PRECAUTIONS 1
Figure 1.2-2 Bar-Code Reader Laser Warning Labels
C A U TI O N
DO N OT ST
T A RE
IGH
RL SE R
I
SE E LA IMUM CL
LA I OD AX
m D ATT M
CAUTION
0n IW
67 MILL
1 .0
LASER LIGHT DO NOT STARE IN TO BEAM
670 nm DIODE LASER

1.0 MILLIWATT MAXIMUM CLASS II LASER PRODUCT
5961298E
Electronic
WARNING Risk of personal injury. Rings or jewelry can contact exposed electronic components, causing
personal injury from electric shock. Remove rings and other metal jewelry before performing maintenance
or service on the electronic components of the instrument.
WARNING Risk of personal injury. Contacting exposed electronic components while the instrument is
attached to power can cause personal injury from electric shock. Power down completely before removing
covers to access electronic components.
CAUTION Risk of damage to electronic components. Connecting or disconnecting a circuit card, solenoid,
or any other electronic component while the power is ON can damage the component or the circuitry for the
component. Turn OFF the instrument before disconnecting or connecting an electronic component.
CAUTION Risk of damage to electronic components. Electrostatic discharge (ESD) can damage disk drives,
add-in circuit cards, and other electronic components. Perform any procedures where there is a possibility
of ESD damage at an ESD workstation, or wear an antistatic wrist strap attached to a metal part of the
chassis connected to an earth ground.
CAUTION Risk of fire hazard. Replacing a fuse of fuse holder with the incorrect type or rating can overheat
the wiring. Always replace a fuse or fuse holder with the type and rating specified for that circuit.
Biological
WARNING Risk of personal injury or contamination. If you do not properly shield yourself before servicing
the MAXM analyzer with the door open, you can be injured or contaminated. To prevent possible injury or
contamination, always wear gloves, a lab coat and eye protection when servicing the instrument with the
doors open.
PN 4235961E 1.2-3
INTRODUCTION
SAFETY PRECAUTIONS
WARNING Risk of contamination. Biohazardous contamination can occur from contact with the waste
container and its associated tubing if not handled with care. Wear protective gear. Avoid skin contact. Clean
up spills immediately. Dispose of the contents of the waste container in accordance with the local
regulations and acceptable laboratory procedures.
Take care when working with pathogenic materials. Means must be available to
decontaminate the instrument, to ventilate air, and to dispose of waste liquid. Refer to the
following publications for further guidance on decontamination.
r Biohazards Safety Guide, 1974, National Institute of Health.

r Classifications of Etiological Agents on the Basis of Hazards, 3d ed., July 1974, Center
for Disease Control, U.S. Public Health Service.
Troubleshooting
Bring the following warning to the customer’s attention before advising that customer to
perform any service, maintenance or troubleshooting procedures on the instrument.
WARNING Risk of personal injury or contamination. If you do not properly shield yourself while performing
service, maintenance and troubleshooting procedures, residual fluids in the instrument can injure or
contaminate you. Coulter recommends barrier protection, such as appropriate safety glasses, lab coat, and
gloves, be worn throughout the performance of service, maintenance and troubleshooting procedures to
avoid contact with cleaners and residual fluids in the instrument.
1.2-4 PN 4235961E
CONTENTS
2
2 INSTRUMENT DESCRIPTION, 2.1-1
2.1 INTRODUCTION TO THE COULTER MAXM ANALYZER, 2.1-1

Function, 2.1-1
Description, 2.1-1
Systems, 2.1-4
Physical Specifications, 2.1-4
Performance Specifications and Characteristics, 2.1-4
Leakage Current Specifications, 2.1-5
Operation, 2.1-5
Modes of Operation, 2.1-5
Operating Procedures, 2.1-6
2.2 ELECTRONIC SYSTEM - OVERVIEW AND POWER SUPPLIES, 2.2-1

Electronic System Overview, 2.2-1
Components, 2.2-1
Function, 2.2-2
Color Coding, 2.2-3
Connectors, 2.2-4
Specifications, 2.2-4
Fuses, 2.2-4
Laser Power Supply, 2.2-4
Function, 2.2-4
RF Power Supply, 2.2-4
Components, 2.2-4
Function, 2.2-4
Voltage Monitoring, 2.2-4
2.3 ELECTRONIC SYSTEM - SIGNAL FLOW BLOCK DIAGRAMS, 2.3-1
2.4 ELECTRONIC SYSTEM - ANALYZER MODULE, 2.4-1

Function, 2.4-1
Description, 2.4-1
376 CPU Card, 2.4-2
Description, 2.4-2
Software Download Sequence, 2.4-2
Jumpers for the 376 CPU Card, 2.4-3
376 CPU Card Inputs/Outputs, 2.4-3
RAM Timer Card, 2.4-3
Jumpers for the RAM Timer Card, 2.4-3
DILUTER INTERFACE Card, 2.4-4
Function, 2.4-4
Lyse Trigger Test Point, 2.4-4
Jumper, 2.4-4
DILUTER INTERFACE Card Inputs/Outputs, 2.4-4
PN 4235961E 2-i
CONTENTS
I/O Card, 2.4-6

Function, 2.4-6
Jumpers, 2.4-7
I/O Card Inputs/Outputs, 2.4-7
VCS PROCESSOR Card, 2.4-8
Function, 2.4-8
Noise Levels, 2.4-9
Jumper, 2.4-9
VCS PROCESSOR Card Inputs/Outputs, 2.4-9
R/W/P PROC Card, 2.4-9
Function, 2.4-9
RBC and WBC Counting Sequences, 2.4-10
RBC and WBC Voting Criteria, 2.4-10
WBC, RBC and Plt Histogram Development, 2.4-10
Data Transfer Method for Histogram Development, 2.4-10
Voting Criteria for Histogram-Derived Parameters, 2.4-13
Noise Levels, 2.4-13
Jumpers, 2.4-13
R/W/P PROC Card Inputs/Outputs, 2.4-13
R/W PREAMP Card, 2.4-14
Function, 2.4-14
R/W PREAMP Card Inputs/Outputs, 2.4-14
2.5 PNEUMATIC SYSTEM, 2.5-1

Pneumatic Power Supply, 2.5-1
Components, 2.5-1
Function, 2.5-1
Fuse, 2.5-2
Pressure and Vacuum Distribution, 2.5-2
Sheath Pressure Distribution, 2.5-3
Sample Pressure Distribution, 2.5-3
High Vacuum Distribution, 2.5-3
Low Vacuum Distribution, 2.5-3
Pneumatic Monitor Card, 2.5-3
2.6 REAGENT SYSTEM, 2.6-1

Reagent Input and Distribution, 2.6-1
Reagent Monitoring, 2.6-1
Fluid Detector/Ram Pressure Card, 2.6-1
Function, 2.6-2
Connectors, 2.6-2
Reagent Temperature Control, 2.6-3
Peltier Module Description, 2.6-3
Peltier Module Function, 2.6-3
Temperature Range Cycles, 2.6-4
Waste Removal and Monitoring, 2.6-5
2-ii PN 4235961E
CONTENTS
2
2.7 SAMPLE HANDLER SYSTEM - SAMPLE HANDLER CARD, 2.7-1
Function, 2.7-1
LEDs, 2.7-1
Responses During Power Up, 2.7-1
Responses During Download, 2.7-3
Responses to the Carousel Position in the Rotary Cap-Pierce Module, 2.7-4
Response to a Power Fluctuation, 2.7-4
Ensuring the Instrument and the Sample Handler Configurations Match, 2.7-4
Avoiding Instrument Damage During a Download, 2.7-4
Jumpers, 2.7-5
Sample Handler Card Inputs/Outputs, 2.7-5
2.8 SAMPLE HANDLER SYSTEM - ROTARY CAP-PIERCE MODULE, 2.8-1

Function, 2.8-1
Description, 2.8-1
RCP Junction Card, 2.8-4
Function, 2.8-4
RCP Junction Card Inputs/Outputs, 2.8-5
2.9 SAMPLE HANDLER SYSTEM - AUTOLOADER MODULE, 2.9-1

Function, 2.9-1
Description, 2.9-1
Autoloader Interface Card, 2.9-7
Function, 2.9-7
Autoloader Interface Card Inputs/Outputs, 2.9-8
Rocker Bed Interface Card, 2.9-8
Function, 2.9-8
Rocker Bed Interface Card Inputs/Outputs, 2.9-9
2.10 SAMPLE HANDLER SYSTEM - AUTOSENSOR TEST CARD, 2.10-1
2.11 SAMPLE PROCESSING SYSTEM - DILUTER, 2.11-1

Function, 2.11-1
Description, 2.11-1
Solenoid Valves, 2.11-1
Blood Sampling Valve (BSV), 2.11-2
Blood/Bubble Detectors, 2.11-3
Sample Processing, 2.11-4
Primary-Mode Aspiration, 2.11-4
WBC/RBC Sample Delivery, 2.11-5
Diff Segmentation, 2.11-6
Diff Segment and Erythrolyse II Reagent Delivery, 2.11-6
Cross Rinse to the Baths, 2.11-7
Secondary-Mode Aspiration, 2.11-7
Pre-Prep/Latex Aspiration, 2.11-8
RBC Sample Delivery, Secondary Mode, 2.11-9
Backwash Functions, 2.11-10
PN 4235961E 2-iii
CONTENTS
2.12 SAMPLE ANALYSIS SYSTEM - CBC TECHNOLOGY, 2.12-1

Summary, 2.12-1
Applying the Coulter Principle, 2.12-1
Aperture Current, 2.12-1
Aperture Vacuum, 2.12-2
Analyzing the RBC, WBC, and Plt Data, 2.12-2
Determining the Hgb, 2.12-2
2.13 SAMPLE ANALYSIS SYSTEM - VCS TECHNOLOGY, 2.13-1

Summary, 2.13-1
VCS Measurements, 2.13-1
V - Volume (DC), 2.13-1
C - Conductivity (RF), 2.13-1
S - Light Scatter (LS), 2.13-1
Opacity (OP), 2.13-2
Rotated Light Scatter (RLS), 2.13-2
Linear Light Scatter (LLS), 2.13-2
Applying the VCS Technology, 2.13-2
DC and RF Currents, 2.13-2
Laser Light Source, 2.13-2
Flow-Cell Hydraulics, 2.13-3
Analyzing the Data, 2.13-5
Preprocessing, 2.13-5
RF Detector Preamp Card, 2.13-5
Function, 2.13-5
RF Detector Preamp Card Inputs/Outputs, 2.13-6
LS Preamp 5 Module, 2.13-6
Primary Function, 2.13-6
Mode Select, 2.13-7
LS Offset Voltage, 2.13-7
LS Preamp 5 Module Inputs/Outputs, 2.13-8
ILLUSTRATIONS
2.1-1 Main Unit with Rotary Cap-Pierce Module, Front View, Main Component
Locations, 2.1-2
2.1-2 Main Unit with Autoloader Module, Front View, Main Component
Locations, 2.1-2
2.1-3 Main Unit Rear View, Main Component Locations, 2.1-3
2.1-4 Main Unit Right-Side View, Main Component Locations, 2.1-3
2.1-5 Main Unit Left-Side View, Main Component Locations, 2.1-4
2.2-2 Electronic Power Supply Components, Top View, Cover Removed, 2.2-3
2.3-1 Signal Flow Block Diagram (MAXM Analyzer with Rotary Cap-Pierce
Module), 2.3-1
2.3-2 Signal Flow Block Diagram (MAXM Analyzer with Autoloader Module), 2.3-2
2-iv PN 4235961E
CONTENTS
2
2.4-1 Circuit Card Locations in the Card Cage, 2.4-1
2.4-2 Initial DMS to Instrument Download, 2.4-2
2.4-3 Subsequent DMS to Instrument Download, 2.4-3
2.4-4 DILUTER INTERFACE Card and Solenoid Junction Card Block Diagram, 2.4-5
2.4-5 I/O Card Block Diagram, 2.4-6
2.4-6 VCS PROCESSOR Card Block Diagram, 2.4-8
2.4-7 R/W/P PROC Card and R/W PREAMP Card Block Diagram, 2.4-11
2.5-1 Pneumatic Power Supply Components, Left-Side View, Compressor Sub-Panel

Removed, 2.5-1
2.5-2 Pressure and Vacuum Air Tank Locations, 2.5-2
2.5-3 Pneumatic Monitor Card Block Diagram, 2.5-4
2.5-4 Monitoring Sequence During Compressor Bleed, 2.5-5
2.6-1 Peltier Controller Card, 2.6-3
2.7-1 Sample Handler Card with Autoloader Module Block Diagram, 2.7-2
2.7-2 Sample Handler Card with Rotary Cap-Pierce Module Block Diagram, 2.7-3
2.8-1 Rotary Cap-Pierce Module Components, 2.8-2
2.9-1 Autoloader Module Components, Front View, Rocker Bed Forward, 2.9-3
2.9-2 Autoloader Module, Front View, Rocker Bed Backward, 2.9-4
2.9-3 Autoloader Module Components, Rear View, 2.9-4
2.10-1 Autosensor Test Card - LEDs Lit Following Power Up, 2.10-1
2.11-1 Solenoid and Tubing Label, 2.11-2

2.11-2 BSV Ports and Wire Markers, 2.11-3
2.11-3 BSV Flow Paths and Wire Markers, 2.11-3
2.11-4 Primary-Mode Aspiration, 2.11-5
2.11-5 WBC/RBC Sample Delivery, 2.11-5
2.11-6 Diff Segmentation, 2.11-6
2.11-7 Diff Segment and Erythrolyse II Reagent Delivery to Mixing Chamber, 2.11-6
2.11-8 Cross Rinse to the Baths, 2.11-7
2.11-9 Secondary-Mode Aspiration, 2.11-8
2.11-10Pre-Prep Latex Aspiration, 2.11-9
2.11-11Sample Delivery, Secondary-Mode, 2.11-9
2.11-12Backwash Functions, 2.11-10
2.12-1 Applying the Coulter Principle, 2.12-1
2.13-1 Applying the VCS Technology, 2.13-3
2.13-2 Light Scatter Path, 2.13-3
2.13-3 Hydrodynamically Focused Cells, 2.13-4
2.13-4 Flow Cell Hydraulics, 2.13-4
2.13-5 RF Detector Preamp Card Signal Flow, 2.13-6
2.13-6 LS Preamp 5 Module Block Diagram, 2.13-7
2.13-7 LS Sensor Regions, 2.13-7
PN 4235961E 2-v
CONTENTS
TABLES
2.6-1 Fluid Detector/Ram Pressure Card LED Positions (Viewing from the Rear of the
Unit), 2.6-2
2.6-2 Ambient Versus Erythrolyse II Reagent Temperatures, 2.6-4
2.6-3 Temperature Range Cycles, 2.6-5
2.8-1 Rotary Cap-Pierce Module - Motors, Solenoids, and Sensors/Switches, 2.8-1
2.9-1 Autoloader Module - Motors, Solenoids, and Sensors/ Switches, 2.9-1
2-vi PN 4235961E
2INSTRUMENT DESCRIPTION 2
2.1 INTRODUCTION TO THE COULTER MAXM ANALYZER
Function
The MAXM analyzer is an automated hematology analyzer and leukocyte differential counter
For In Vitro Diagnostic Use in clinical laboratories. The MAXM analyzer reports a complete
blood count (CBC) and white blood cell differential from whole blood.
The CBC consists of a white blood cell count (WBC), red blood cell count (RBC),
hemoglobin (Hgb), mean corpuscular volume (MCV), hematocrit (Hct), mean corpuscular
hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), red cell
distribution width (RDW), platelet count (Plt) and mean platelet volume (MPV).
The WBC differential consists of the percent and number of the following WBC populations:
lymphocytes (LY% and LY#), monocytes (MO% and MO#), neutrophils (NE% and NE#),
basophils (BA% and BA#) and eosinophils (EO% and EO#).
The MAXM analyzer with Reticulocyte analysis also reports the percent and number of
reticulocytes (RET% and RET#) from prepared whole-blood samples.
In addition, the MAXM analyzer generates platelet and reticulocyte parameters that are “For
Research Use Only. Not For Use In Diagnostic Procedures.” These parameters are the
plateletcrit (Pct), and the platelet distribution width (PDW), the mean reticulocyte cell
volume (MRV), and the maturation index (MI).
For a description of the methodologies used to derive the CBC, and to derive the diff and retic
parameters, refer to Chapter 3 of the customer’s Reference manual.
Description
Main Components
The MAXM analyzer consists of the Main Unit and the Data Management System (DMS). The
Main Unit comprises the:
r Electronic Power Supply

r Pneumatic Power Supply
r Analyzer/Systems Control module (commonly referred to as the Analyzer module)
r Sample Handler. Two Sample Handler modules are available, the Rotary Cap-Pierce
module and the Autoloader module.
r Diluter. The Diluter is further divided into six modules -
t BSV module
t CBC module
t Main Diluter module
t Mixing module
t Flow-Cell module (commonly referred to as the Triple Transducer module or TTM)
t Pump module
Figures 2.1-1 through 2.1-5 show the locations of the main components/modules in the
Main Unit.
The DMS comprises the computer and its monitor, keyboard, and software. For details about
the DMS, see the DMS Configuration Listing for STKS™, MAXM™, and HmX Series Systems.
PN 4235961E 2.1-1
INSTRUMENT DESCRIPTION
INTRODUCTION TO THE COULTER MAXM ANALYZER
Figure 2.1-1 Main Unit with Rotary Cap-Pierce Module, Front View, Main Component Locations
Analyzer
module
CBC
module
BSV module
Electronic
(new configuration)
Power Supply
Diluter
CA
UT
IO
N
Rotary Cap-
Pierce module
Pump
L O
C K
MK6
MF7
module
5961240E
Figure 2.1-2 Main Unit with Autoloader Module, Front View, Main Component Locations
Analyzer CBC
module module
BSV module
Electronic (old configuration)
Power Supply
Diluter
16 17 18 19
MF2
Autoloader
module
Pump
MK6
MF7 module
M A N U ALS .
E R LY .R E FE R TO P R O D U C T
A S S Y . D IS P O S E O F P R O P -
H AN D LE O R S TO R E N EE D LE
IN J U R Y : U S E S A F E T Y C L IP
T O IN S T A L L . R E M O V E ,
B IO H A Z A R D /S H A R P
O B J E C T . T O A V O ID
5961241E
2.1-2 PN 4235961E
INTRODUCTION TO THE COULTER MAXM ANALYZER 2
Figure 2.1-3 Main Unit Rear View, Main Component Locations
Fluid Detector/ Laser

Ram Pressure card Power
(rear, closest to door) Supply
Pneumatic
Monitor
card (front)
POWER
switch
Electronic
Power
Supply
Pneumatic
Power
Supply
5961264D
Figure 2.1-4 Main Unit Right-Side View, Main Component Locations
Main Diluter
module
Mixing
module LASER
ON
Flow Cell
module
5961247D
PN 4235961E 2.1-3
Figure 2.1-5 Main Unit Left-Side View, Main Component Locations
Fuse
panel
Sample
Handler
Pneumatic card
Power
Supply
5961238D
Systems
The MAXM analyzer comprises several systems, each performing a specific function. This
chapter includes a description of the following systems.
r Electronic (See Headings 2.2, 2.3, and 2.4.)

r Pneumatic (See Heading 2.5.)
r Reagent (See Heading 2.6.)
r Sample Handler (See Headings 2.7, 2.8, 2.9, and 2.10.)
r Sample Processing (See Heading 2.11.)
r Sample Analysis (See Headings 2.12 and 2.13.)
Physical Specifications
Refer to Chapter 4 of the customer’s Reference manual for physical specifications and power
requirements.
Performance Specifications and Characteristics

See Appendix A, TOLERANCES AND LIMITS, for the performance specifications needed to
service the MAXM analyzer. For any other performance specifications or performance
characteristics (including interfering substances), refer to Chapter 4 of the customer’s
Reference manual.
2.1-4 PN 4235961E
INTRODUCTION TO THE COULTER MAXM ANALYZER 2
Leakage Current Specifications
Coulter instruments are evaluated and approved to Underwriters Laboratories Standard 1262,
Laboratory Equipment. Testing was performed by Electrical Testing Laboratories (ETL).
Leakage current is tested per Section 6.7 - EXCEPTION:
Equipment required to have primary circuit filtering to meet c (EMC) regulations may
have a Leakage Current at accessible parts of more than 500 µA, but must not exceed
5.0 mA.
Coulter instruments such as the MAXM analyzer and its peripherals incorporate primary
circuit filtering to meet EMC requirements, therefore the exception in UL Standard 1262,
Section 6.7 is applicable and allows a leakage current as high as 5.0 mA.
Computer systems, such as the DMS, and their peripherals are evaluated and approved to
Underwriters Laboratories Standard 478, Information Processing and Business Equipment,
prior to being approved for use with Coulter instruments. Leakage current is tested per
Section 28 A, paragraph 28 A.3, EXCEPTION:
A unit that requires electromagnetic interference filters or filtering capacitors or both for
functional performance or to meet EMC regulations (for example FCC regulations) may
have Leakage Current in excess of 500 µA but must not exceed 3.5 mA.
Computers and their peripherals used with the MAXM analyzer employ EMC filtering devices
as described in UL 478 Section 28 A, paragraph 28 A.3, EXCEPTION; therefore the maximum
allowable leakage current is 3.5 mA Diagnostic Products
Refer to Chapter 4 of the customer’s Reference manual for the diagnostics products used on
the MAXM analyzer.
Operation
Modes of Operation
In the Primary mode the MAXM analyzer uses one of two options to introduce sample from a
closed specimen tube into the system, a rotary cap-piercing mechanism (Rotary Cap-Pierce
module) or a specimen transporting and cap-piercing mechanism (Autoloader module).
Instruments that have the Rotary Cap-Pierce module operate when the specimen tube is
identified by a bar-code label. The label is read by presenting the specimen tube to a bar-code
reader before the tube is placed in the cap-pierce carousel. Only one specimen tube can be
placed in the cap-pierce carousel at a time.
Instruments that have the Autoloader module begin to function when a cassette containing
specimen tubes is placed in the loading bay. The Autoloader module transports the cassette to
the piercing station, where the specimen tube and cassette positions are identified by the
bar-code reader. When the labels have been read, the specimen tube cap is pierced by the
needle, and a sample is aspirated into the instrument.
In addition to the cap-piercing Primary mode, a Secondary mode is available for manually
submitting single, open-vial samples. On instruments with the reticulocyte option, this mode
is also used to analyze whole-blood samples prepared for reticulocyte analysis.
PN 4235961E 2.1-5
Operating Procedures
Refer to the customer’s Operator's Guide for the operating procedures.
2.1-6 PN 4235961E
ELECTRONIC SYSTEM - OVERVIEW AND POWER SUPPLIES 2
2.2 ELECTRONIC SYSTEM - OVERVIEW AND POWER SUPPLIES
Electronic System Overview
Input voltage is supplied to the Main Unit via the ac input cable on the rear of the Electronic
Power Supply. Turning ON the POWER switch on the rear of the Electronic Power Supply
routes the ac input voltage to the Electronic Power Supply.
In the Electronic Power Supply, the ac voltage is routed to the 5.6 V supply which provides
power for battery protection, and to the K1 relay. If the Standby/Ready switch is on
Standby (O), K1 is open, stopping flow. If the Standby/Ready switch is on Ready (I), K1 is
closed and the ac voltage is routed through K1 to the remaining power supplies and to the
peripheral outlet on the back of the Main Unit. For details, refer to the Power Supply Module
schematic, DCN 6320500 or DCN 6320750, in Chapter 6, SCHEMATICS.
The power supplies convert the ac input voltage to dc voltages which are routed from the
Electronic Power Supply, via connectors on the top of the Electronic Power Supply, to the TB
(terminal board) on the rear of the Analyzer module. From the terminal board the voltages
are distributed through the main wiring harness to the analog and digital backplanes in the
card cage and to the Diluter modules. For details, refer to the Interconnect Diagram
MAXM/HmX Systems, DCN6320619, in Chapter 6, SCHEMATICS.
Electronic Power Supply

The Electronic Power Supply (EPS) is a self-contained unit located below the Analyzer
module in the Main Unit. See Figures 2.1-1, 2.1-2, and 2.1-3 for location.
Components
The Electronic Power Supply consists of the following (see Figures 2.2-1 and 2.2-2 for
component locations):
r Five power supply modules:

t PS3: ±15 Vdc and +5 Vdc
t PS4: +24 Vdc
t PS5: RF +6.3 Vdc and +300 Vdc
t PS6: +12 Vdc
t PS7: +5.6 Vdc.
Note: All supply voltages are adjustable.
r Test points (J120) accessible at the front of the Electronic Power Supply for measuring
the dc voltages.
r A switch for 120/220 V selection.
r Connectors for all voltage inputs and outputs.
r A compressor solid-state relay, K2, that connects the input ac voltage to the compressor.
r An ac line filter that filters the input ac voltage before going to the transformer.
r A cooling fan.
PN 4235961E 2.2-1
ELECTRONIC SYSTEM - OVERVIEW AND POWER SUPPLIES
Function
The Electronic Power Supply converts the ac input voltage to the following dc voltages:
r +5 Vdc
r +5.6 Vdc
r +6.3 Vdc
r +12 Vdc
r ±15 Vdc
r +24 Vdc
r +240 Vdc
r +300 Vdc.
Figure 2.2-1 Electronic Power Supply, Front View

J113 J114 J107 J108 J112 J116 J115 J119 J105
VOLTAGE TEST
-15v
+5v +12v
+5.6v +6.3v
+15v GND
+24v
UNIT CONFIGURATION
100 120 220 240 CAUTION

VAC VAC VAC VAC ELECTRIC SHOCK
HAZARD. PANEL
TO BE OPENED
BY QUALIFIED
50/60 HZ SERVICE PER-
FRONT SECTION CENTER SECTION REAR SECTION ONEL ONLY.
J120 5961219D
2.2-2 PN 4235961E
ELECTRONIC SYSTEM - OVERVIEW AND POWER SUPPLIES 2
Figure 2.2-2 Electronic Power Supply Components, Top View, Cover Removed
Cooling
fan
DANGER
Voltage
connectors
12 10
12
3
3
J119
J114
J116
J108
TB6 and TB7 under
15
3
10
1
1
1
J105
connector
J115
6
3
6
3
J113
J112
13
1
TB1 panel
J107
1
1
4
4
1
1
120/220 V Aperture
selection Voltage/
switch Power Fail
Detect card
120V
PS5
RF Power
Supply
+6.3 Vdc
+300 Vdc
PS3 +5 Vdc
PS6 and ±15 Vdc
+12 Vdc
TB5 PS4 TB4 TB2 PS7 TB3

+24 Vdc +5.6 V 5961035D
Color Coding
Throughout the MAXM analyzer, the following wire colors are used for the Electronic Power
Supply voltages:
r Black = ground
WARNING Risk of personal injury. The blue and brown wires are used for both ac and dc voltages and
might carry high voltage. To avoid an electric shock:
r Use due care when working around any electronic components with the power ON.
r Power down completely before connecting or disconnecting electronic components.
r Blue = +12 Vdc (Blue is also used for the neutral line of the main ac wiring.)
r Brown = +24 Vdc (Brown is also used for the hot line of the main ac wiring.)
r Green = -15 Vdc
r Orange = +15 Vdc
r White = +5.6 Vdc
r Yellow = +5 Vdc
PN 4235961E 2.2-3
ELECTRONIC SYSTEM - OVERVIEW AND POWER SUPPLIES
Connectors
The power from the Electronic Power Supply is distributed to the rest of the Main Unit
through connectors on the top of the Electronic Power Supply (Figure 2.2-1). The voltages
supplied at each connector are listed in Heading A.3-4, Electronic Power Supply Test Points
and Adjustments.
Specifications
For the required operating temperature range and the storage temperature and range, refer to
Chapter 4 of the customer’s Reference manual.
Voltage Configurations
Refer to Table A.3-3, Electronic Power Supply Terminal Card Jumpers and Connections.
Fuses
Refer to Figure 2.1-5 for the location of the fuse panel on the Electronic Power Supply. The
fuse ratings and functions are listed in Table A.3-2, Fuse Ratings and Functions.
Laser Power Supply

The Laser Power Supply is mounted on the right rear door of the instrument (left/right
orientation from the front of the instrument). See Figure 2.1-3 for location.
Function
The Laser Power Supply has an input of 100/115/230 Vac (from the Electronic Power Supply)
and supplies high voltage (1350 Vdc) to operate the laser. Refer to Table A.3-5, Laser Power
Supply Buck-Boost Transformer Jumpers and Connections, for voltage configurations.
RF Power Supply
Components
The RF power supply, located inside the Electronic Power Supply housing (Figure 2.2-2),
consists of:
r The RF transformer, an assembly with two transformers, RF and aperture current

r The RF Power Supply card.
Function
The RF transformer converts the main ac input voltage to 150 Vac. The 150 Vac is then sent
to the RF Power Supply card where it produces 300 Vdc for the RF Detector Preamp card, if
the RF SUPPLY ON signal is low (active low).
The filament voltage (6.3 Vdc) for the vacuum tube in the RF Detector Preamp card is also
produced by the RF Power Supply card. The input voltage of +24 Vdc from the +24 Vdc
power supply is converted to an output of 6.3 Vdc.
Voltage Monitoring
The I/O card monitors all system voltages including the Laser Power Supply and RBC and
WBC aperture voltages.
2.2-4 PN 4235961E
ELECTRONIC SYSTEM - SIGNAL FLOW BLOCK DIAGRAMS 2
2.3 ELECTRONIC SYSTEM - SIGNAL FLOW BLOCK DIAGRAMS
Figure 2.3-1 illustrates the electronic signal flow for the MAXM analyzer with Rotary
Cap-Pierce module, Figure 2.3-2 for the MAXM analyzer with Autoloader module. Shading in
a block indicates the circuit card is on the data bus.
Figure 2.3-1 Signal Flow Block Diagram (MAXM Analyzer with Rotary Cap-Pierce Module)
DMS
VIA
DIGIBOARD
376 CPU
CARD
DILUTER SOLENOID
RF DETECTOR INTERFACE JUNCTION
NMI RF
PREAMP CARD CARD
POWER
CARD
SUPPLY
PNEUMATIC
POWER
PNEUMATIC SUPPLY
MONITOR
CARD FLOW CELL
DILUTER
FLUID SWITCHES
FAN DETECTOR/RAM R
PRESSURECARD R/W/P
PROC W
CARD P SOLENOIDS
PELTIER
AMBIENT MODULE
TEMP
APERTURE
SENSOR
LAMPS
HGB
PREAMP
SAMPLE
BLOOD
HANDLER
DETECTORS
ZAP CARD
LASER
POWER I/O CARD
SUPPLY BAR-CODE
READERDE RCP
CODER JUNCTION
R/W CARD
APER VOLT/
RETIC HGB PREAMP
LASER POWER FAIL
INTERFACE LAMP CARD
DETECT CARD
BAR-CODE
RBC WBC SCAN SENSORS
BATH BATH
VCS
LS SENSOR LS PREAMP PROCESSOR
CARD
CAROUSEL
LEDS
5961065D MOTOR
PN 4235961E 2.3-1
ELECTRONIC SYSTEM - SIGNAL FLOW BLOCK DIAGRAMS
Figure 2.3-2 Signal Flow Block Diagram (MAXM Analyzer with Autoloader Module)
DMS
VIA
DIGIBOARD
376 CPU
CARD
DILUTER SOLENOID
NMI RF DETECTOR INTERFACE JUNCTION
RF CARD
PREAMP CARD
POWER
CARD
SUPPLY
PNEUMATIC
POWER
PNEUMATIC
SUPPLY
MONITOR
CARD FLOW CELL
DILUTER
FLUID DETECTOR/ SWITCHES
FAN RAM PRESSURE R
CARD R/W/
PPROC W
CARD P SOLENOIDS
PELTIER
AMBIENT MODULE
TEMP
SENSOR APERTURE
HGB LAMPS
PREAMP
BLOOD
SAMPLE
DETECTORS
ZAP HANDLER
LASER CARD
POWER I/O CARD
SUPPLY
BAR-CODE
READER
DECODER AUTOLOADER
APER VOLT/ R/W INTERFACE
HGB
POWER FAIL PREAMP CARD
LASER RETIC LAMP
DETECT CARD CARD
INTERFACE
BAR-CODE
SCAN
RBC WBC SENSORS
BATH BATH
VCS
LS SENSOR LS PREAMP PROCESSOR
CARD ROCKER BED
MOTORS INTERFACE
5961112D CARD
For details on the components included in Figures 2.3-1 and 2.3-2, refer to the following
headings or tables.
For information on... See...

376 CPU card 376 CPU Card under Heading 2.4, ELECTRONIC SYSTEM - ANALYZER MODULE
Autoloader Interface card Autoloader Interface Card under 2.9, SAMPLE HANDLER SYSTEM - AUTOLOADER
MODULE
Blood detectors Main Components, Blood/Bubble Detectors, under Heading 2.11, SAMPLE
PROCESSING SYSTEM - DILUTER
DILUTER INTERFACE card DILUTER INTERFACE Card under Heading 2.4, ELECTRONIC SYSTEM - ANALYZER
MODULE
Fluid Detector/Ram Pressure card Fluid Detector/Ram Pressure Card under Heading 2.6, REAGENT SYSTEM
I/O card I/O Card under Heading 2.4, ELECTRONIC SYSTEM - ANALYZER MODULE
Laser Power Supply Laser Power Supply under Heading 2.2, ELECTRONIC SYSTEM - OVERVIEW AND
POWER SUPPLIES
LS Preamp module LS Preamp 5 Module under Heading 2.13, SAMPLE ANALYSIS SYSTEM - VCS
TECHNOLOGY
Peltier module Reagent Temperature Control, Peltier Module Description, under Heading 2.6,
REAGENT SYSTEM
2.3-2 PN 4235961E
ELECTRONIC SYSTEM - SIGNAL FLOW BLOCK DIAGRAMS 2
For information on... See...
Pneumatic Monitor card Pneumatic Monitor Card under Heading 2.5, PNEUMATIC SYSTEM
Pneumatic Power Supply Pneumatic Power Supply under Heading 2.5, PNEUMATIC SYSTEM
R/W PREAMP card R/W PREAMP Card under Heading 2.4, ELECTRONIC SYSTEM - ANALYZER
MODULE
R/W/P PROC card R/W/P PROC Card under Heading 2.4, ELECTRONIC SYSTEM - ANALYZER
MODULE
RCP Junction card RCP Junction Card under Heading 2.8, SAMPLE HANDLER SYSTEM - ROTARY
CAP-PIERCE MODULE
RF Detector Preamp card RF Detector Preamp Card under Heading 2.13, SAMPLE ANALYSIS SYSTEM - VCS
TECHNOLOGY
RF Power Supply RF Power Supply under Heading 2.2, ELECTRONIC SYSTEM - OVERVIEW AND
POWER SUPPLIES
Rocker Bed Interface card Rocker Bed Interface Card under 2.9, SAMPLE HANDLER SYSTEM - AUTOLOADER
MODULE
Sample Handler card Heading 2.7, SAMPLE HANDLER SYSTEM - SAMPLE HANDLER CARD
Sensors Table 4.43-2, Switch/Sensor Checks on the MAXM Analyzer with Rotary Cap-Pierce
Module, or Table 4.43-1, Switch/Sensor Checks on the MAXM Analyzer with
Autoloader Module
Solenoids Table 4.26-1, Solenoid Operations
VCS PROCESSOR card VCS PROCESSOR Card under Heading 2.4, ELECTRONIC SYSTEM - ANALYZER
MODULE
PN 4235961E 2.3-3
ELECTRONIC SYSTEM - SIGNAL FLOW BLOCK DIAGRAMS
2.3-4 PN 4235961E
ELECTRONIC SYSTEM - ANALYZER MODULE 2
2.4 ELECTRONIC SYSTEM - ANALYZER MODULE
Function
The Analyzer module controls the timing and sequencing of the operating cycles, receives
and analyzes the CBC and VCS pulses and raw data from the Diluter (counts, measures and
computes parameters), and sends the information to the DMS.
Note: While the components in the Analyzer module could be divided and discussed
separately as parts of the Electronic System, System Control, and the Sample Analysis system,
for convenience they are discussed here as a group under the Electronic System.
Description
The Analyzer module is located above the Electronic Power Supply in the Main Unit. See
Figure 2.1-1 or Figure 2.1-2 for location. The Analyzer module comprises an EMC shield, a
card cage, and six circuit cards inserted into the card cage (Figure 2.4-1).
The card cage includes the Digital backplane, the Analog backplane, and the Solenoid
Junction card, which provide the signal and power interfacing for the Analyzer module’s
circuit cards.
CAUTION Inserting a circuit card in the wrong slot in the card cage could damage the instrument. Use
caution when inserting cards.
Figure 2.4-1 Circuit Card Locations in the Card Cage The circuit cards inserted into
DILUTER VCS R/W/P the Analyzer module’s card cage
INTERFACE PROCESSOR PROC
are (Figure 2.4-1):
r 376 CPU (slot 2)

r DILUTER INTERFACE
W
(slot 22)
H
LYSE
TIMMING
A
P
T
r I/O (slot 32)
E
R
T r VCS PROCESSOR (slot 37)
WADJ
WIA r R/W/P PROC (slot 42)

CONTROL RIA
LYSE TRIG VOLUUM
RADJ r R/W PREAMP (slot 54).
V
MIX
H
C
GRD
L
S
R
GND E
GND
D
A
P
L PROBE E
R
T
376 CPU I/O R/W

PREAMP
5961036D
PN 4235961E 2.4-1
ELECTRONIC SYSTEM - ANALYZER MODULE
376 CPU Card

Refer to Figure 2.4-1 for the location of the 376 CPU card in the Analyzer card cage.
Description
The 376 CPU card has an 80376 CPU running at 16 MHz, with a 80387 math coprocessor. It
has a 1-MB EPROM, used as a boot PROM, and 1.3 MB of RAM. The 1.3 MB of RAM is
protected by a lithium battery that must have a value of approximately 3.6 V. Any value below
3.55 V indicates a weak battery.
The card uses an 82370 controller that controls the DMA (Direct Memory Access), Interrupts,
and System Timers.
Software Download Sequence

Download occurs between the DMS and the 376 CPU card. The status of the instrument’s
onboard RAM depends on the software that is loaded.
On the initial download, once the DMS has completed its boot sequence, the download
(Figure 2.4-2) begins in this order:
1. The DMS sends the instrument code to the 376 CPU card.
2. The DMS sends the instrument configuration to the 376 CPU card.
3. The DMS sends the Sample Handler code to the 376 CPU card.
4. The DMS sends the diluter tables to the 376 CPU card. At the same time, the 376 CPU
card sends the Sample Handler code to the Sample Handler card.
5. The DMS sends the Sample Handler configuration to the 376 CPU card.
Figure 2.4-2 Initial DMS to Instrument Download

1. INSTRUMENT CODE
2. INSTRUMENT CONFIGURATION
3. SAMPLE HANDLER CODE 376 CPU
DMS
CARD
4. DILUTER TABLES
5. SAMPLE HANDLER CONFIGURATION
4. SAMPLE
HANDLER
CODE
SAMPLE
HANDLER
CARD
5961113C
On subsequent reboots, once the DMS has completed its boot sequence, the download
(Figure 2.4-3) begins in this order:
1. The DMS sends the instrument configuration to the 376 CPU card.
2. The DMS sends the Sample Handler configuration to the 376 CPU card.
2.4-2 PN 4235961E
3. Since the Sample Handler code has been previously downloaded to the 376 CPU card
and stored in RAM, the 376 CPU card sends the Sample Handler code to the Sample
Handler card while the DMS is booting.
Figure 2.4-3 Subsequent DMS to Instrument Download
1. INSTRUMENT CONFIGURATION
376 CPU
DMS CARD
2. SAMPLE HANDLER CONFIGURATION
3. SAMPLE
HANDLER
CODE
SAMPLE
HANDLER
CARD
5961114C
Jumpers for the 376 CPU Card

The 376 CPU card has 22 jumper locations. See Figure A.2-1 and Table A.2-1 for the correct
jumper configuration.
376 CPU Card Inputs/Outputs
Inputs: Outputs:
r Electronic Power Supply r Digiboard in the DMS

r Digiboard in the DMS r I/O card
r Aperture Voltage/Power Fail Detect card r R/W/P PROC card
in the Electronic Power Supply r VCS PROCESSOR card
r I/O card r DILUTER INTERFACE card
r R/W/P PROC card r Solenoid Junction card
r VCS PROCESSOR card r Sample Handler card via the Solenoid
r DILUTER INTERFACE card Junction card
r Solenoid Junction card
r Sample Handler card via the Solenoid
Junction card
RAM Timer Card

The RAM Timer card contains additional RAM memory for the 376 CPU card. The RAM
Timer card sits on top of the 376 CPU card and is connected to it. Refer to Figure A.2-8.
Jumpers for the RAM Timer Card

The RAM Timer card has two jumper locations. See Figure A.2-8 for the correct jumper
configuration.
PN 4235961E 2.4-3
DILUTER INTERFACE Card

Refer to Figure 2.4-1 for the location of the DILUTER INTERFACE card in the Analyzer
card cage.
Function
Refer to the block diagram, Figure 2.4-4, as you read this section. Shading in a block indicates
the circuit card is on the data bus.
The DILUTER INTERFACE card controls:
r The 300 Vac to the RF Detector Preamp card.

r The COMPRESSOR ON signal to the Electronic Power Supply (which turns on the
Pneumatic Power Supply).
r The 72 solenoids through the Solenoid Junction card.
r The aperture-viewing lamps.
r The mix motor.
The DILUTER INTERFACE card monitors the FLOW DETECT, RF DETECT, and CLOG
DETECT signals from the RF Detector Preamp card.
Lyse Trigger Test Point

The test point used for triggering when performing diluent and lytic reagent timing, TP26, is
located on the front of the DILUTER INTERFACE card and is labeled LYSE TRIG. Refer to
Figure A.2-3.
Jumper
The DILUTER INTERFACE card has one jumper location. See Figure A.2-3 for the correct
DILUTER INTERFACE Card Inputs/Outputs
Inputs: Outputs:
r Electronic Power Supply r 376 CPU card

r 376 CPU card r Solenoid Junction card
r Solenoid Junction card r Pneumatic Power Supply
r RF Detector Preamp card r Solenoids
r Diluter switches r RF Detector Preamp card
r Aperture optic lamps
Troubleshooting tip: DILUTER INTERFACE Card Inputs/Outputs lists the direct inputs
and outputs for the card. Be aware however, that any defective circuit card on the Digital
backplane could affect the operation of the DILUTER INTERFACE card.
2.4-4 PN 4235961E
Figure 2.4-4 DILUTER INTERFACE Card and Solenoid Junction Card Block Diagram
67,68, and 70
DIGITAL GND
DIGITAL GND
DIGITAL GND
APER LAMP
CMPSR ON
LV 41-50
LV 35-52
LV 51-59
+24 VDC
LV 69
P21 1 10 11 12 13 21 23 29 30 31 32
P20 1
2 LV21-22
1 P19 5
LV1-8 LV25-30
10
+24 VDC for
8 11 LV31-34,
+24 VDC for
11 12 54-59, and 65
LV14-22
12 13
16 LV31-40
LV14-20
Solenoid Junction card 22
LV65 MIX ON
22 23
MIX MOTOR
24 LV66 RF ON
25 RF PWR SPLY
RF DETECT 29 26 LV67
RF/DC PREAMP LV68
CLOG DETECT 30
SHEATH TANK J24

31 WHOLE BLOOD
29 SWITCH
+24 VDC for 43 1 2 3
LV1-8 RINSE BLOCK
30
DOWN
44 RINSE BLOCK
31
HOME
+24 VDC
GND
+24 VDC
DIGITAL GND
DILUTER
INTERFACE
card
376 DIGITAL BACKPLANE DATA BUS

CPU
card J14 J13 J11 J10/J12
1 2 3 4 1 2 3 4 1 2 1 2 3
I/O VCS R/W/P

ANALOG GND
+15 VDC
ACLO (POWER FAIL)
+5.6 VDC
-15 VDC
+5 VDC
+5 VDC
+5 VDC
+5 VDC
DIGITAL GND
DIGITAL GND
DIGITAL GND
DIGITAL GND
PROCESSOR PROC
card card card
5961050D
PN 4235961E 2.4-5
I/O Card
Refer to Figure 2.4-1 for the location of the I/O card in the Analyzer card cage.
Function
Figure 2.4-5 I/O Card Block Diagram
I/O card
P7
+15 VDC
-15 VDC
+5 VDC
1
+5 VDC
2
3 +5 VDC
J13
DIGITAL GND
4
DIGITAL GND LASER MON +5.28 = +1350 VREF
4A
1 RF MON +4.7 VDC = 300 VREF
+5 VDC 5A
2 +6.3 VDC MON Laser
3 +5 VDC 6A Power
J14
DIGITAL GND SHEATH PRESS Supply
4 7A
DIGITAL GND 1 DIFF PRESS
+15 VDC 8A
3 30 PSI RF
+5 VDC 9A Pneumatic
6 Monitor Power
-15 VDC 60 PSI
7 10A card Supply
+240 VDC J18 LOW VAC
8 11A
ANALOG GND
2 HI VAC
12A
4 LS OFFSET VLTG LS
5 14A preamp
LYSE TEMP
2A
AMB TEMP
Analog backplane bus
DILUTER 3A
INTERFACE Peltier
COOL PELTIER Controller
card 16A
Digital backplane bus
HEAT PELTIER module

17A
PELTIER ENABLE
18A
HGB LAMP VLTG Hgb
19A
HGB LAMP GND lamp
20A
HGB SIGNAL
21A
DIFF/ HGB SHIELD GND Hgb
VCS 22A preamp
PROCESSOR RETIC +12 VDC MON
card SELECT 23A
PELTIER READY
25A
26A WASTE
R/W/P PAK LYSE
PROC 27A EPS
card PAK PRESERVE
28A
DILUENT Fluid
29A
ZAP LYSE Detector/ Waste
30A Ram Pressure
CLEANER float
RIA MON 31A card sensor
R/W OVERFLOW DET
PREAMP WIA MON 32A
card DIFF/RETIC SELECT
RIA ON 9C
+24 V MON Retic
WIA ON 31C interface
harness
1
J10 +15
2
& -15
3
J12 ANALOG GND
EPS
376
CPU
card
5961049D
2.4-6 PN 4235961E
The I/O card monitors and measures the following system status conditions and converts the
analog inputs to digital signals for use by the 376 CPU card:
r The system pressures and vacuums -

t 30 psi, 60 psi, sheath, and diff (sample) pressures
t High and low vacuums
r The optical or float reagent level sensors, waste sensor, and CBC overflow sensors
r The LS offset voltage
r The ambient temperature and the reagent temperature in the Peltier module’s ceramic
reagent manifold
r All system voltages including the Laser Power Supply, the RBC and WBC aperture, the
Hgb blank, and the Hgb sample voltages
The I/O card controls:
r Diff/Retic select
r Red and White aperture current control
r ZAP command
r Peltier module temperature using the AUTO, COOL, and HEAT signals
In addition, the I/O card houses the DAC (digital to analog converter) for the Hgb regulator.
The 376 CPU card controls the Hgb lamp through the I/O card.
Jumpers
The I/O card has two jumper locations. See Figure A.2-5 for the correct jumper configuration.
I/O Card Inputs/Outputs
Inputs: Outputs:
r Voltage from Electronic Power Supply r 376 CPU card
r 376 CPU card r VCS PROCESSOR card
r R/W PREAMP card r R/W PREAMP card
r Laser Power Supply r Hgb lamp
r RF Power Supply r Peltier module
r Pneumatic Monitor card r Retic interface harness
r Peltier module
r Light scatter preamp
r Hgb preamp
r Waste float sensor
r Fluid Detector/Ram Pressure card
Troubleshooting tip: I/O Card Inputs/Outputs lists the direct inputs and outputs for the
card. Be aware however, that any defective circuit card on the Digital backplane could affect
the operation of the I/O card.
PN 4235961E 2.4-7
VCS PROCESSOR Card

Refer to Figure 2.4-1 for the location of the VCS PROCESSOR card in the Analyzer card cage.
Function
Figure 2.4-6 VCS PROCESSOR Card Block Diagram

VCS PROCESSOR card
DC IN ANALOG
DC PEAK
DIGITAL
AMP DETECT
+15 VDC CONVERTER
+5 VDC
-15 VDC
ANALOG
RF IN RF PEAK DIGITAL
AMP DETECT CONVERTER
LS IN LS ANALOG
PEAK DIGITAL
AMP DETECT CONVERTER
1 DIFF RETIC
+15 VDC
3 SELECT
+5 VDC MON
-15 VDC 6 J18
ANALOG GND 8
2 1
4 +5 VDC
5 +5 VDC 2
3 J13
J55 DIGITAL GND
DIGITAL GND 4
RF-
3
+5 VDC 1
RF RF+
4 2
Detector DC- +5 VDC
3 J14
Preamp 5 DIGITAL GND
card DC+ DIGITAL GND 4
6
DILUTER
INTERFACE
J49 card
LS+
LS 1 I/O card
Preamp
LS-
module 2
R/W/P
PROC
card
R/W 376
PREAMP CPU
card card
5961052D
2.4-8 PN 4235961E
The VCS PROCESSOR card is interfaced to the backplane via control, address, and data
signals to and from the 376 CPU card.
The LS Preamp module and RF Detector Preamp card pass pulses to the VCS PROCESSOR
card, which further amplifies the signals with three separate amplifiers: one each for volume
and conductivity, and one for scatter (Diff or Retic). Only one scatter gain is in use at a time,
depending on whether Diff or Retic is selected. Three independent A/D converters convert the
peak pulses to a 12-bit value which is sent to the 376 CPU card via direct memory access
(DMA).
Noise Levels
Three test points on the front of the VCS PROCESSOR card (Figure A.2-13) allow
measurement of the electronic noise levels on the V, C, and S channels. Refer to Diff and Retic
RMS Noise Checks under Heading 4.12, RMS NOISE CHECKS, for the procedure.
Jumper
The VCS PROCESSOR card has one jumper location. See Figure A.2-13 for the correct
VCS PROCESSOR Card Inputs/Outputs
Inputs: Outputs:
r 376 CPU card r V, C, S test points on the front of the
r I/O card card
r LS Preamp module
r RF Detector Preamp card
Troubleshooting tip: VCS PROCESSOR Card Inputs/Outputs lists the direct inputs and
outputs for the card. Be aware however, that any defective circuit card on the Digital
backplane could affect the operation of the VCS PROCESSOR card.
R/W/P PROC Card

Refer to Figure 2.4-1 for the location of the R/W/P PROC card in the Analyzer card cage.
Function
The R/W/P PROC card contains circuitry both for counting RBCs and WBCs, and for
developing the RBC, WBC, and Plt histograms. While these circuits are contained on the
same circuit card and are used simultaneously, they function independently of each other.
PN 4235961E 2.4-9
RBC and WBC Counting Sequences

The R/W/P PROC card receives amplified RBC, WBC, and Plt pulses from the R/W
(Red/White) PREAMP card; see Figure 2.4-7. The counting circuits count RBC and WBC
particle pulses for 12 seconds, consisting of three 4-second count periods (Count Periods 1,
2, and 3).
The results from each count period are transferred via the data bus to the 376 CPU card where
they are coincidence-corrected, voted on, averaged, and multiplied by a calibration factor.
RBC and WBC Voting Criteria

The voting criteria for both the RBC and WBC parameters are:
r If the Hgb value is >1.0 g/dL, and if the result obtained from Count Period 1 is "voted
out," then the final parameter result is "-----," which indicates a total voteout of the
result. Similarly, if there is a total voteout of the three count periods (complete
disagreement among all count period results), then the final parameter result is "-----."
r The counts from at least two of the three count periods must agree to obtain a final result
(according to the above criteria).
r If the count from either count period 2 or 3 votes out, then the final parameter result
represents the average of the two remaining count periods.
r If a parameter value results from the average of only two count periods, then the result is
flagged with a "*V."
WBC, RBC and Plt Histogram Development

The MAXM analyzer develops the WBC, RBC, and Plt histograms separately from the WBC
and RBC counts, using the R/W PREAMP, the R/W/P PROC, and the 376 CPU cards.
r The R/W PREAMP card amplifies the WBC, RBC, and Plt particle pulses and outputs
them to the R/W/P PROC card.
r The R/W/P PROC card edits the WBC, RBC and Plt pulses based on an acceptable pulse
height and width criteria (pulse editing). The R/W/P PROC converts the edited analog
pulses to a digital address (analog-to-digital conversion), then transfers the digital data
to the 376 CPU card via the Digital backplane (data bus).
r The 376 CPU card stores the transferred digital data, formulates the WBC, RBC, and Plt
histograms, and provides histogram analysis necessary to determine the MCV, RDW, Plt,
MPV, and PDW parameters.
The DMS determines the percentages of the LY, MO, NE, BA and EO populations, and
calculates the absolute numbers of these populations.
Data Transfer Method for Histogram Development

For troubleshooting purposes, it is important to note that the particle pulse accumulation
method for the WBC, RBC, and Plt histograms does not follow the same 1, 2, and 3 Count
Period sequence as the WBC and RBC "count."
2.4-10 PN 4235961E
Figure 2.4-7 R/W/P PROC Card and R/W PREAMP Card Block Diagram
RIA MON
WIA MON
+5 VDC +5 VDC
+15 VDC +15 VDC
R/W/P
-15 VDC -15 VDC PROC
R/W PREAMP card
card +240 VDC
ZAP COMMAND
RED PULSES AMPLIFIED RED PULSES
PLT PULSES AMPLIFIED PLT PULSES
WHITE PULSES AMPLIFIED WHITE PULSES
RIA ON
+5 VDC
WIA ON
1
+5 VDC
2
+5 VDC J13
3
DIGITAL GND
DIGITAL GND 4
RBC WBC
BATH BATH
1
+5 VDC
2
+5 VDC J14
3
DIGITAL GND
DIGITAL GND 4
1
+15 VDC
3
+5 VDC
-15 VDC 6 J18 VCS
7 PROCESSOR
+240 VDC card
8
ANALOG GND
DILUTER
INTERFACE
card
ZAP COMMAND
RIA MON
WIA MON I/O
RIA ON card
WIA ON
376
CPU
5961051D card
For example, the RBC particle pulses accumulated during Count Period 1 (4 seconds) are not
used to exclusively formulate the corresponding RBC Histogram 1; similarly, the pulses
accumulated during Count Periods 2 or 3 are not used exclusively to formulate RBC
Histogram 2 or 3.
PN 4235961E 2.4-11
Instead, the MAXM analyzer uses a unique method of transferring particle pulse data to form
each parameter’s histogram. The method is as follows:
T = 1 2 3 4 5 6 7 8 9 10 11 12c
H = 1 2 3 1 2 3 1 2 3 1 2 3
where
T = Number of seconds within the standard accumulation period (1st second, 2d

second, and so forth)
H = Number of the corresponding histogram into which the accumulated data
is transferred.
The system always accumulates particle pulses for histogram development for a minimum of
12 seconds as illustrated above. However, additional accumulation time may be required to
meet the following criteria:
r Each WBC histogram must accumulate a minimum of 2,000 pulses per histogram, or
must have completely filled at least one channel within each 256-channel histogram.
r Each RBC histogram must accumulate a minimum of 5,000 pulses per histogram, or
must have completely filled at least one channel within each 256-channel histogram.
r Any Plt histogram accumulates a minimum of 800 pulses.
At the end of the initial 12-second accumulation time, if the above criteria are not met for any
given histogram, the MAXM analyzer proceeds into an extended accumulation phase:
T = 13 14 15c 16 17 18c 19 20 21c 22 23 24cc

H = 1 2 3 1 2 3 1 2 3 1 2 3
where
T = Number of seconds within the extended accumulation time frame (13th

second, 14th second, and so forth).
H = Number of the corresponding histogram into which the accumulated data is
transferred (WBC, RBC, and Plt histograms 1, 2, and 3).
c = Point at which the CPU checks to determine if the accumulation criteria have
been met. If the criteria are met, the extended accumulation sequence stops.
cc = Marks the maximum accumulation time allowed for the WBC histogram. The
WBC aperture current is turned off, and vacuum is no longer applied to the
WBC aperture.
2.4-12 PN 4235961E
If the RBC or Plt histogram accumulation criteria are not satisfied after 24 seconds, the
system continues the extended accumulation phase:
T = 25 26 27c 28 29 30c 31 32 33c 34 35 36cc

H = 1 2 3 1 2 3 1 2 3 1 2 3
where
T = Number of seconds within the extended accumulation time frame (25th

second, 26th second, and so forth).
H = Number of the corresponding histogram into which the accumulated data is
transferred (RBC and Plt histograms 1, 2, and 3).
c = Point at which the CPU checks to see if the accumulation criteria have been
met. If the criteria are met, then the extended accumulation sequence stops.
cc = Marks the maximum accumulation time allowed for the RBC and Plt
histograms. The RBC aperture current is turned off, and the vacuum is no
longer applied to the RBC aperture. Accumulation stops.
Voting Criteria for Histogram-Derived Parameters

The voting criteria for histogram-derived parameters, MCV, RDW, Plt, and MPV are similar to
the voting criteria for the RBC and WBC. The only exception is if the result obtained from the
corresponding histogram 1 votes out. Then the final result reflects the average of the
remaining two histogram results (no "-----" due to histogram 1 result vote out).
Noise Levels
Three test points and a ground on the front of the R/W/P PROC card (Figure A.2-10) allow
measurement of the electronic noise levels (RMS) on the RBC, WBC and Plt channels. Refer
to CBC RMS Noise Checks under Heading 4.12, RMS NOISE CHECKS, for the procedure.
The noise limits are specified in Electronic Noise Limits under Heading A.1, TOLERANCES
AND LIMITS.
Jumpers
The R/W/P PROC card has eleven jumper locations. See Figure A.2-10 for the correct jumper
configuration.
R/W/P PROC Card Inputs/Outputs
Inputs: Outputs:
r R/W PREAMP card r RED, WHT and PLT test points on the
r 376 CPU card front of the card
Troubleshooting tip: R/W/P PROC Card Inputs/Outputs lists the direct inputs and outputs
for the card. Be aware however, that any defective circuit card on the Digital backplane
could affect the operation of the R/W/P PROC card.
PN 4235961E 2.4-13
R/W PREAMP Card

Refer to Figure 2.4-1 for the location of the R/W PREAMP card in the Analyzer card cage.
Function
The R/W PREAMP card contains:
r The aperture current regulators.

r The burn circuitry (zap) for the apertures. The I/O card issues the ZAP COMMAND to
the R/W PREAMP card.
The R/W PREAMP card supplies the aperture current to the RBC and WBC baths. The RBC,
WBC, and Plt pulses are fed through the R/W PREAMP card where they are amplified to an
acceptable level to be processed by the R/W/P PROC card
R/W PREAMP Card Inputs/Outputs
Inputs: Outputs:
r Electronic Power Supply r R/W/P PROC card
r Aperture Voltage/Power Fail Detect card
r RBC bath
r WBC bath
r I/O card
2.4-14 PN 4235961E
PNEUMATIC SYSTEM 2
2.5 PNEUMATIC SYSTEM
The pneumatic system develops, monitors, and distributes the pressures and vacuums needed
to operate the system.
Pneumatic Power Supply

The Pneumatic Power Supply is a self-contained unit located at the bottom of the Main Unit
and accessible from both the rear (Figure 2.1-3) and the left side (Figure 2.1-5) of the
Main Unit.
Components
The Pneumatic Power Supply includes (see Figure 2.5-1 for component locations):
r A dual-head air compressor/vacuum pump driven by a single electric motor.

r A cooling fan that draws air over a condensing coil and away from the pump.
r An adjustable, 60 psi, pressure relief valve.
Figure 2.5-1 Pneumatic Power Supply Components, Left-Side View, Compressor Sub-Panel Removed
Air compressor/
vacuum pump
60 psi
regulator
pressure
relief valve
Cooling
Shipping pins
fan 5961032D
Function
The compressor/vacuum pump produces one adjustable level of air pressure, 60 psi, and one
nonadjustable level of vacuum, >22 in. of Hg. (The exact level of vacuum varies with
altitude.) The 60-psi air pressure and the vacuum are supplied to the system modules for
operation of the pneumatic and hydraulic components.
PN 4235961E 2.5-1
PNEUMATIC SYSTEM
Voltage Configurations
The Pneumatic Power Supply has a buck-boost transformer installed in series with the ac
input line. This transformer is used to adjust the ac input voltage to the optimum voltage
required by the Pneumatic Power Supply. See Table A.3-6, Pneumatic Power Supply
Buck-Boost Transformer Terminal Card Jumpers and Connections, for instructions on
configuring the buck-boost transformer wiring.
Fuse
The Pneumatic Power Supply has one fuse, located on the rear of Pneumatic Power Supply.
The fuse rating and function is listed in Table A.3-2, Fuse Ratings and Functions.
Pressure and Vacuum Distribution

For distribution details, refer to the Pneumatic/Hydraulic Layout, DCN 6320510, in
Chapter 6.
60-psi Pressure Distribution

Unregulated pressure from the compressor/vacuum pump is routed to a pressure-relief valve
in the Pneumatic Power Supply (Figure 2.5-1), which regulates the pressure to 60 psi. From
the pressure relief valve, the 60-psi pressure is routed through a water trap to an air tank (on
later model MAXM analyzers only, see Figure 2.5-2), which maintains the 60-psi pressure at
an acceptable level during peak loads. From the air tank, or from the water trap on
instruments without an air tank, the 60-psi pressure is routed to:
r The Pneumatic Monitor card for monitoring.

r The 30-psi regulator, RG2, for regulation to 30 psi.
r LV43 for dispensing any condensation collected in the water trap to waste.
Figure 2.5-2 Pressure and Vacuum Air Tank Locations
Right side door

(open)
Pressure
tank
High vacuum
tank
5961287D
2.5-2 PN 4235961E
PNEUMATIC SYSTEM 2
30-psi Pressure Distribution
The 30 psi pressure is routed to:

r Manifolds for distribution to solenoids and various pneumatic components. 30 psi is
used to activate pinch valves, move cylinders, dispense reagents from pumps, and drain
waste from the instrument.
r The sheath pressure regulator, RG3, for regulation of the sheath pressure.
r The sample pressure regulator, RG4, for regulation of the sample pressure.
Sheath Pressure Distribution

The sheath pressure is distributed to:

r The sheath tank to push the sheath fluid from the sheath tank through the flow cell,
creating the diluent sheath in the flow cell.
r Solenoids LV7 (WBC) and LV8 (RBC) to generate mixing bubbles in the aperture baths.
Sample Pressure Distribution

The sample pressure is distributed to:

r The Mixing module to push sample from the mixing chamber through the flow cell,
creating the sample stream in the flow cell.
High Vacuum Distribution

High, unregulated vacuum from the compressor/vacuum pump is routed through the vacuum
trap to a vacuum chamber for distribution to the:
r The Pneumatic Monitor card for monitoring and to a vacuum tank (Figure 2.5-2). The
vacuum tank maintains the high vacuum at an acceptable level during peak loads, even
at high altitudes.
r The low vacuum regulator, RG1, for regulation to 6.0 in. of Hg.
r Various pneumatic components. High vacuum is used to drain the aperture baths and the
mixing chamber, refill pumps, refill the sheath tank, and refill the bubble trap, VC2, for
the sweep-flow line.
Low Vacuum Distribution

r The vacuum isolator chambers. The vacuum in the vacuum isolator chambers pulls the
dilutions through the apertures, and pulls sweep-flow diluent behind the RBC aperture.
Pneumatic Monitor Card

The Pneumatic Monitor card is located on the upper rear Diluter door. See Figure 2.1-3 for
location.
PN 4235961E 2.5-3
PNEUMATIC SYSTEM
The Pneumatic Monitor card monitors the 60 psi, 30 psi, Hi Vac, Low Vac, sheath pressure,
and diff (sample) pressure. See Figure 2.5-3.
Note: Although the sample pressure is routed to the Pneumatic Monitor card, this card does
not actually monitor the sample pressure; it monitors the difference between the sheath
pressure and the sample pressure, the diff pressure. To accomplish this, sheath pressure is
routed to two ports on the Pneumatic Monitor card, each monitored by a transducer.
r One of the transducers compares the sheath pressure to atmosphere, to report the actual
sheath pressure.
r The second transducer compares the sheath pressure to the sample pressure routed to
the DIFF port, to report the diff pressure.
Figure 2.5-3 Pneumatic Monitor Card Block Diagram

P1
30 PSI
1
60 PSI
2
HI VAC
3
SHEATH P7
4
Pneumatic LOW VAC
Monitor 5 7A
DIFF
card 6 8A
8 -15 VDC 9A
I/O card
9 +15 VDC 10A
10 ANALOG GND 11A
12A
5961120D
Six test points (TP100 through TP600) on the card, corresponding to each pressure or
vacuum, provide access for external testing or monitoring. The test points are measured
in Vdc. Refer to Table A.2-6 for the conversion from psi to Vdc for each test point. Signals
from the Pneumatic Monitor card are sent to the I/O card.
The Pneumatic Monitor card monitors the 30-psi and 60-psi pressure during a compressor
bleed so that the instrument software can control solenoids relative to the bleeding sequence
and determine if the compressor is at a ready state for operation. Refer to Figure 2.5-4 for a
diagram detailing the sequence of events.
Note: During power up, the MAXM analyzer monitors vacuum and pressure. If these values
are out of the specified range, then the MAXM analyzer does not complete the power-up
sequence. The Download Not Successful error message appears. (A specific vacuum or pressure
error message does not appear.)
To determine the cause of the Download Not Successful message, externally monitor the
vacuum and pressure measurements at the test points on the Pneumatic Monitor card and
make the appropriate adjustments if the voltage value is out of range. Refer to Heading 4.25,
PRESSURE/VACUUM ADJUSTMENT, for measurement and adjustment procedures.
2.5-4 PN 4235961E
PNEUMATIC SYSTEM 2
Figure 2.5-4 Monitoring Sequence During Compressor Bleed
Turn
compressor
off.
Activate vacuum
bleed LV37
and
compressor bleed
LV68.
As compressor
bleeds, begin
monitoring
30/60 psi.
Deactivate bleed LV37 Did

and LV68. Generate 30/60 psi
COMPRESSOR DID NOT NO bleed down to 15 psi
BLEED error. Deactivate within 60
Peltier and discontinue seconds?
monitoring.
YES
Deactivate compressor
bleed LV68. Activate
(open) water trap
LV43 via LV70.
Deactivate (close) water Did

trap. Generate WATER 30/60 psi
TRAP DID NOT BLEED NO bleed below 2 psi
error. Deactivate within 60
Peltier and discontinue seconds?
monitoring.
YES
Software waits
additional 3 seconds then
deactivates vacuum bleed
LV37 and closes water trap
LV43 via LV70.
Activate compressor
bleed LV68, turn
compressor on.
Software waits
2 seconds then
deactivates bleed
LV68.
NO
Did Turn off compressor.

Is this the Generate COMPRESSOR
Discontinue YES 30/60 psi recover NO second attempt to YES
monitoring and to operating range PRESSURE ERROR or
recover the 30/60 psi 30 PSI OUT OF RANGE error.
activate Peltier within 60 operating
seconds? Deactivate Peltier and
range? discontinue monitoring.
5961119D
PN 4235961E 2.5-5
PNEUMATIC SYSTEM
2.5-6 PN 4235961E
REAGENT SYSTEM 2
2.6 REAGENT SYSTEM
Reagent Input and Distribution
The MAXM analyzer uses five reagents. To fully understand the function of each of these
reagents, read the Method History section and the Reagents section in Chapter 1 of the
customer’s Reference Manual.
r Diluent - to dilute the blood for analysis and to rinse the system between samples
r CBC lytic reagent - to disrupt the RBCs for WBC analysis and to convert the Hgb for
measurement
r Diff lytic reagent - to disrupt the RBCs while maintaining the WBCs in a near native state
for analysis of the WBC subpopulations
r Diff leukocyte preservative - to preserve the WBCs in a near native state for analysis of
the WBC subpopulations
r Cleaning agent - to clean the system and prevent protein buildup on the apertures.
One end of each reagent input line is attached to a pickup tube inserted into the reagent
container, the other end to a fitting on the rear of the Main Unit. During the instrument
cycles, the reagents are pulled from the reagent containers into the back of the instrument
and to the respective reagent pumps or tanks.
Reagent Monitoring
Older models of the MAXM analyzer use pickup tubes with float sensors in the diluent and
cleaning agent to monitor the reagent levels, and cycle counters to keep track of the CBC lytic
reagent and PAK reagent usage.
When the diluent or cleaning agent drops too low, activating the float sensor, or the
instrument cycle counter for the CBC lytic reagent or PAK counts down to five remaining
cycles, the instrument generates a 5 Cycles Left warning message, then 4 Cycles Left, etc. until
it halts after 1 Cycle Left. At that point, the operator must change the reagent, update the
reagent log, and prime the instrument to resume operating.
On newer models of the MAXM analyzer, the reagents flow through optical sensors mounted
behind the reagent panel, inside the Main Diluter module. Reagents in the optical sensors
transmit light, but air prevents light transmission. When the instrument runs out of a reagent
and starts pulling bubbles through the reagent’s optical sensor, the instrument halts
immediately and generates a "reagent out" error message. No warning messages are
generated. The operator must change the reagent, update the reagent log, and prime the
instrument to resume operating.
Fluid Detector/Ram Pressure Card

The Fluid Detector/Ram Pressure card is mounted between the rear Diluter door and the
Pneumatic Monitor card. See Figure 2.1-3 for location. The component side of the card faces
the rear Diluter door.
PN 4235961E 2.6-1
REAGENT SYSTEM
Function
The Fluid Detector/Ram Pressure card contains circuitry to support the five optical reagent
sensors. The Fluid Detector/Ram Pressure card monitors the status of the following:
r RBC bath overflow

r WBC bath overflow
r CBC lytic reagent
r PAK LYSE (Erythrolyse II reagent)
r PAK PRESERVE (StabiLyse reagent)
r Diluent
r Cleaning agent
A bank of LEDs (seen through the vent slots on the upper rear Diluter door) is mounted on
the component side of the Fluid Detector/Ram Pressure card. See Table 2.6-1.
Table 2.6-1 Fluid Detector/Ram Pressure Card LED Positions (Viewing from the Rear of the Unit)
Position 10 9 8 7 6 5 4 3 2 1
Function Not used Not used Spare Cleaning Diluent StabiLyse Erythrolyse II CBC lytic WBC RBC
agent reagent reagent reagent bath bath
These LEDs can be used to visually verify the status of each of the optical reagent sensors and
bath overflow detectors.
LED ON = Reagent is sensed or bath overflow condition not detected.
LED OFF = Reagent is not sensed or bath overflow condition is detected.
To troubleshoot a status discrepancy, after verifying liquid is present in the respective sensor,
determine if the card or sensor is at fault by swapping connections of sensor inputs with a
known working sensor.
r If the status discrepancy follows the sensor, replace the sensor.

Note: If a new sensor is not immediately available, you can bypass the reagent sensor via
software to make the instrument operational. If you do bypass the reagent sensor, ensure
the operator is aware the reagent level is not being monitored and restore the reagent
monitoring function as soon as possible.
r If the status discrepancy does not follow the sensor, replace the Fluid Detector/Ram
Pressure card.
Note: You must reboot the instrument each time to display the new status.
Connectors
The Fluid Detector/Ram Pressure card connectors are shown in Figure A.2-4 and described in
Table A.2-3.
2.6-2 PN 4235961E
REAGENT SYSTEM 2
Reagent Temperature Control
Peltier Module Description

The Peltier module, PLB1, is located on the rear of the BSV module. See Figure A.5-3 for
location.
The Peltier module consists of:
r A Peltier Controller card. See Figure A.5-3 for location. See Figure 2.6-1 for connectors,
signals, and LEDs.
r A ceramic reagent manifold with input and output fittings.
r A set of Peltier cells mounted against the ceramic reagent manifold.
r A thermistor mounted inside the ceramic reagent manifold.
r An ambient temperature sensor mounted on the front of the BSV module. See
Figure A.5-1 or Figure A.5-2 for location.
Figure 2.6-1 Peltier Controller Card

Current output to
Peltier cells biased
for heating or
cooling functions.
ENABLE AUTO
from I/0 card J
Peltier 2
Controller
ENABLE HEAT card
or COOL from
I/O card
Ambient
temperature J
from ambient 1
temperature
sensor
Ceramic reagent
manifold temperature
from thermistor J
CR1 CR3 CR2 3
CR1 = Not ready, stabilizing

CR2 = Heating
CR3 = Cooling 5961163D
Peltier Module Function

The Peltier module allows the instrument to monitor and adjust the temperature of the
reagents used in the diff mixing chamber to obtain an optimum chemical reaction in the
mixing chamber.
PN 4235961E 2.6-3
REAGENT SYSTEM
The Erythrolyse II reagent and the mixing chamber rinse (diluent from the sheath tank) are
routed through the ceramic reagent manifold on their way to the diff mixing chamber. When
the Peltier cells are electronically activated into either the Heating mode or the Cooling mode,
the Peltier cells heat or cool the ceramic reagent manifold which, in turn, heats or cools the
reagents flowing through it.
The ambient temperature sensor monitors the ambient room temperature. The Peltier module
tracks the ambient room temperature and heats or cools the reagents in the ceramic reagent
manifold in inverse proportion to changes in that temperature; when the ambient room
temperature decreases, the Peltier module heats the reagents in the ceramic reagent manifold,
and when the ambient room temperature increases, the Peltier module cools the reagents.
The instrument software compares the temperature in the ceramic reagent manifold to the
ambient room temperature to determine if the Peltier module is performing correctly.
Table 2.6-2 shows the temperature that the Erythrolyse II reagent should be, ±2.8°C,
depending on the ambient room temperature.
Table 2.6-2 Ambient Versus Erythrolyse II Reagent Temperatures
Erythrolyse II Erythrolyse II Erythrolyse II Erythrolyse II

Ambient Reagent Ambient Reagent Ambient Reagent Ambient Reagent
Temp °C Temp °C Temp °C Temp °C Temp °C Temp °C Temp °C Temp °C
12.8 47.5 20.0 31.7 25.6 19.5 32.8 3.6
13.3 46.3 20.6 30.5 26.1 18.3 33.3 2.4
13.9 45.1 21.1 29.3 26.7 17.1 33.9 1.2
14.4 43.9 21.7 28.0 27.2 15.8 34.4 0.0
15.0 42.7 22.2 26.8 27.8 14.6 35.0 -1.2
15.6 41.4 21.7 28.0 28.3 13.4 34.4 0.0
16.1 40.2 22.2 26.8 28.9 12.2 35.0 -1.2
16.7 39.0 22.8 25.6 29.4 11.0 35.6 -2.5
17.2 37.8 23.3 24.4 30.0 9.7 36.1 -3.7
17.8 36.6 23.9 23.2 30.6 8.5 36.7 -4.9
18.3 35.3 24.4 21.9 31.1 7.3 37.2 -6.1
18.9 34.1 25.0 20.7 31.7 6.1 37.8 -7.3
19.4 32.9 32.2 4.9
Temperature Range Cycles

The Peltier Controller card, I/O card, and 376 CPU card work together to control the
operation of the Peltier module based on the ambient room temperature. The software
controlling the Peltier module includes different mixing cycles for four temperature ranges.
Table 2.6-3 lists the four temperature ranges and describes the differences in the
mixing cycles.
2.6-4 PN 4235961E
REAGENT SYSTEM 2
Table 2.6-3 Temperature Range Cycles
Temperature
Range No. °C °F Temperature Range Cycles

1 13.3 - 20.5 56 - 69 Before sample delivery, the mixing chamber receives a pre-rinse of Erythrolyse II
reagent and a full rinse of heated diluent via the Peltier module.
The mixing cycle is 2.5 seconds.
reagent via the Peltier module.
reagent and a half rinse of cooled diluent via the Peltier module.
reagent and a full rinse of cooled diluent via the Peltier module.
Waste Removal and Monitoring

During the cycle, waste is collected in chambers in the Main Unit and drained through the
waste line on the rear of the Main Unit.
When a waste container is used, the I/O card monitors a waste float sensor located inside the
pickup tube in the waste container to prevent backup into the instrument. When waste is
routed directly to a drain, the waste level sensor is bypassed with a jumper.
PN 4235961E 2.6-5
REAGENT SYSTEM
2.6-6 PN 4235961E
SAMPLE HANDLER SYSTEM - SAMPLE HANDLER CARD 2
2.7 SAMPLE HANDLER SYSTEM - SAMPLE HANDLER CARD
The Sample Handler card is located in the left compartment of the MAXM analyzer. See
Figure 2.1-5 for location.
Either of two Sample Handler cards, the Sample Handler I or the Sample Handler II card can
be used, depending on the Sample Handler module installed.
r The original Sample Handler I card can only be used on the MAXM analyzer with Rotary
Cap-Pierce module. It is not compatible with the MAXM analyzer with Autoloader
module.
r The newer Sample Handler II card can be used on either the MAXM analyzer with
Autoloader module or the MAXM analyzer with Rotary Cap-Pierce module, but the
MAXM analyzer with Rotary Cap-Pierce module must have the RCP Junction II card.
Function
Refer to the block diagrams, Figure 2.7-1 and Figure 2.7-2. Shading in a block indicates the
circuit card is on the data bus.
The Sample Handler card contains an 80196 micro-controller that communicates with the
376 CPU card via an RS-422 serial connection.
The Sample Handler card:
r Interfaces the blood/bubble detectors and the Bar-Code Reader Decoder card to the
376 CPU card.
r Controls the Autoloader or Rotary Cap-Pierce module.
LEDs
Four LEDs on the side of the Sample Handler card light for a designated purpose during
power up, reset, instrument cycles, and download. From top to bottom, the four LEDs are:
r CPU (CR4)
r ROM (CR3)
r RAM (CR2)
r I/O (CR1).
Responses During Power Up

On power up, the four LEDs light, indicating the on-board tests have begun. Starting at the
top with CPU, each LED extinguishes as the on-board tests pass. If an LED fails to extinguish,
it indicates a failure of this portion of the card, and the card locks up. For example, if the
ROM LED does not extinguish, the card has failed the ROM on-board tests. If the card locks
up, reboot the system.
If the on-board power-up tests pass, the I/O LED flashes, indicating the Sample Handler card
has entered the "wait for download mode." The I/O LED flashes at approximately one flash
per second indicating that it does not have the Sample Handler code, and that it is waiting for
the DMS to download the Sample Handler code.
PN 4235961E 2.7-1
SAMPLE HANDLER SYSTEM - SAMPLE HANDLER CARD
Figure 2.7-1 Sample Handler Card with Autoloader Module Block Diagram
376
CPU
card
Solenoid Junction
card
P22 RS-422 Serial

P33 interface Front
+12 VDC blood/bubble
1
GND J13 detector
2
J3 +24 VDC 5
J14
GND 6
Rear
ANLG GND Sample Handler card blood/bubble
1 detector
+5 VDC
3
+12 VDC
J2 +15 VDC
4
-15 VDC 5 -12 VDC Bar-Code
Reader
DIGTL GND 6 J5 +5 VDC
Decoder
J15 P43 RS232 P44 card
Load stack J6
empty sensor S15 J2 P16
J9 J19
Autoloader Interface Card J10
Unload stack Bar-code
J4 J11
full sensor J20 scanner
S11 J3 J5 J6 J14 J15 J18 J17
Needle Stop switch

Tube ram Rocker bed
home sensor S4
sensor S10 motor, M1
S0
LED ON = home
LV83
Needle Door
tube ram
LV81 interlock
forward sensor
tube return S5
S1
LV82
Rocker bed pierce needle
Tube horizontal position
LV84 forward sensor J13 sensor S17
bed lock S14 J16
Rocker bed
pierce position
Tube available J12 sensor S16
sensor S7
J31 Cassette index
J42
Stepper motor J34 motor M2
J32 Rocker Bed Interface card
unloading elevator
J33
motor B2 J39 Full cassette
J37 J35 J41 J38 J43 J40 index rotator
Stepper motor sensor S9
loading elevator Load elevator Cassette position 0 Cassette position 2
motor B1 down sensor S8 sensor S12 sensor S3
Unload elevator Cassette position 1 Cassette position 3

down sensor S6 sensor S2 sensor S13 5961221D
2.7-2 PN 4235961E
Figure 2.7-2 Sample Handler Card with Rotary Cap-Pierce Module Block Diagram
376
CPU
card
Solenoid Junction
card
P22 RS-422 Serial

P23 interface Front
+12 VDC blood/bubble
1 J1 J13 detector
GND
2
J3 +24 VDC J14
5
GND Rear
6 blood/bubble
detector
ANLG GND Sample Handler card
1
+12 VDC
+5 VDC 3
-12 VDC Bar-Code
J2 +15 VDC
4 +5 VDC Reader
-15 VDC 5 Decoder
RS232
DIGTL GND 6 J5 J6 card
P43 P44
J15
RCP LEDS P16

green J2 J3 Bar-code
RCP Junction card scanner
RCP LEDS
J4 J1
red
LV24
needle cylinder Normal pierce
Stepper sensor U3
motor
LV23
tube clamp Oversize pierce
Needle forward sensor U4
sensor U2
Door
Hand detector LEDs
interlock
J8 P8 Needle home DS1 transmit and
sensor U1 Q2 receive
Bar code detector LEDs

Tube in position DS2 transmit and
sensor U5 Q1 receive 5961222D
Responses During Download

When the Sample Handler card detects the start of a flash download, the I/O and RAM LEDs
light indicating the Sample Handler card I/O is receiving the download from the DMS and is
placing the code into RAM.
During the download if a communication problem develops, the CPU and I/O LEDs light
indicating the Sample Handler card software is attempting to abort the download. The Sample
Handler card remains in this mode until the DMS aborts the download.
PN 4235961E 2.7-3
During or after download if the lower front door is opened, the CPU LED flashes and the
instrument is immediately put into a protect mode.
After the download, if a cap-pierce sensor is defective or out of alignment, the LEDs go out
and the Sample Handler card locks up until the sensor problem is corrected. On MAXM
analyzers with a Rotary Cap-Pierce module, the red LED near the tube entry port lights,
indicating system halt. If a lock-up occurs, reboot the system.
After the download is successfully completed, on MAXM analyzers with a Rotary Cap-Pierce
module the LEDs are used as described below in Responses to the Carousel Position in the
Rotary Cap-Pierce Module.
Responses to the Carousel Position in the Rotary Cap-Pierce Module

After the Sample Handler code is downloaded, the LEDs indicate the different locations of the
carousel by lighting the LEDs according to the carousel location as it rotates.
r Top LED - carousel is at HOME position.

r Second LED - carousel is between HOME and PIERCE position.
r Third LED - carousel is at PIERCE position.
r Bottom LED - carousel is between PIERCE and HOME position.
Response to a Power Fluctuation

If a power fluctuation occurs, the AC LOW DETECT signal is asserted. This change of state
resets the 376 CPU card. The 376 CPU card simultaneously sends a RESET command to the
Sample Handler card. After the Sample Handler card resets, it performs the functions listed
above under Responses During Power Up, causing it to lose the Sample Handler code stored
in RAM. The I/O LED flashes at approximately one flash per second, indicating that it does
not have the Sample Handler code. The 376 CPU card automatically downloads the Sample
Handler code whenever there is a power problem.
Ensuring the Instrument and the Sample Handler Configurations Match

When the system is booted, the status and/or presence of the STOP switch on the lower front
door indicates to the Sample Handler card if the hardware of the MAXM analyzer is
configured as an Autoloader or a Rotary Cap-Pierce module. During system initialization, this
"Hardware Signature" must match the Sample Handler "Style" entered at the DMS when the
enhanced software package was initially installed. If the two do not match, the system fails to
operate and the following error message appears on the DMS during software installation:
Inconsistent Sample Handler Hardware. Check hardware -- Reinstall Software.
Avoiding Instrument Damage During a Download

During a download, while the system is booting and before completion of the Sample Handler
code download, a Gate Array Logic (GAL) chip, U11, on the Sample Handler card provides
hazard control features to prevent potential damage to the Autoloader or Rotary Cap-Pierce
modules. For example, if the needle is not retracted during the download, the GAL does not
allow moving parts to cycle; instead, the needle retracts.
2.7-4 PN 4235961E
Jumpers
The Sample Handler I card has 27 jumper locations (X1 - X27). See Figure A.2-11 for the
correct jumper configuration.
The Sample Handler II card has 7 jumper locations (X28 - X34). See Figure A.2-12 for the
correct jumper configuration.
Sample Handler Card Inputs/Outputs

Inputs: Outputs:
r Electronic Power Supply r Solenoid Junction card

r Solenoid Junction card r Bar-Code Reader Decoder card
r Front blood detector r On MAXM analyzer with Autoloader
r Rear blood detector module: Autoloader Interface card
r Bar-Code Reader Decoder card r On MAXM analyzer with Rotary
Cap-Pierce module: RCP Junction card
r On MAXM analyzer with Autoloader
module: Autoloader Interface card
r On MAXM analyzer with Rotary
Cap-Pierce module: RCP Junction card
PN 4235961E 2.7-5
2.7-6 PN 4235961E
SAMPLE HANDLER SYSTEM - ROTARY CAP-PIERCE MODULE 2
2.8 SAMPLE HANDLER SYSTEM - ROTARY CAP-PIERCE MODULE
Function
The Rotary Cap-Pierce module is a closed vial, cap-piercing mechanism, capable of accepting
one specimen tube at a time for introducing a sample into the instrument. For the location of
the Rotary Cap-Pierce module, see Figure 2.1-1.
Description
The Rotary Cap-Pierce module consists of:
r A bar-code reader for identifying the specimen tubes. See Figure 2.8-1
r A carousel assembly for receiving the specimen tubes from the operator and moving the
specimen tubes into position for piercing. See Figure 2.8-1
r A needle assembly for piercing the specimen tubes for sample aspiration. See
Figure 2.8-1
r A tube ejector and exit tray for collecting the specimen tubes for retrieval by the
operator. See Figure 2.8-1
r Motors, solenoids, cylinders, and sensors/switches for performing and monitoring the
functions of the Rotary Cap-Pierce module.
All the motors (B = stepper motors), solenoids (LV), and sensors/switches (U) associated
with the Rotary Cap-Pierce module functions, except the blood/bubble detectors, are
listed in Table 2.8-1, Rotary Cap-Pierce Module - Motors, Solenoids, and Sensors/
Switches. The locations of these components are shown in Figure 2.8-1.
For a description of the blood/bubble detectors, see Blood/Bubble Detectors under
Heading 2.11, SAMPLE PROCESSING SYSTEM - DILUTER.
r An interface card, the RCP Junction card, for interfacing the components in the Rotary
Cap-Pierce module with the Sample Handler card. The Rotary Cap-Pierce module is
controlled via the Sample Handler card.
t For details about the RCP Junction card, see RCP Junction Card at the end of this
section.
t For details about the Sample Handler card, see Heading 2.7, SAMPLE HANDLER
SYSTEM - SAMPLE HANDLER CARD.
Table 2.8-1 Rotary Cap-Pierce Module - Motors, Solenoids, and Sensors/Switches
Functional
Item Description Function Style Location
B1 Carousel motor Rotates carousel position determined by Stepper motor Figure 2.8-1, carousel
U1 - U5. assembly
LV23 Tube clamp solenoid Controls position of the clamp cylinder, CL4. Solenoid Figure 2.8-1, lower left
front of module
LV24 Needle cylinder Controls position of the needle cylinder, CL5. Solenoid Figure 2.8-1, lower left
solenoid front of module
DS1/ Hand detector LEDs This two-part sensor, DS1 transmit (on the Transmitter/ Figure 2.8-1, carousel
Q2 right) and Q2 receive (on the left), checks for receiver assembly
objects blocking carousel rotation before the
carousel is rotated.
PN 4235961E 2.8-1
SAMPLE HANDLER SYSTEM - ROTARY CAP-PIERCE MODULE
Table 2.8-1 Rotary Cap-Pierce Module - Motors, Solenoids, and Sensors/Switches (Continued)
Functional
Item Description Function Style Location
DS2/ Bar-code detector This two part sensor, DS2 transmit (on the Transmitter/ Figure 2.8-1, carousel
Q1 LEDs left) and Q1 receive (on the right), senses the receiver assembly
presence of a tube and activates the bar-code
laser scanner.
U1 Needle home Senses when the needle is retracted to the Optical sensor Figure 2.8-1, next to
home position. or the needle assembly
Hall effect (optical) or on CL5
sensor (Hall effect).
U2 Needle forward Senses when the needle is extended. Optical sensor Figure 2.8-1, next to
or the needle assembly
Hall effect (optical) or on CL5
sensor (Hall effect).
U3 Normal position Senses a normal sized specimen tube is in the Optical sensor Figure 2.8-1, on code
pierce position. wheel
U4 Oversized position Senses an oversized specimen tube is in the Optical sensor Figure 2.8-1, on code
pierce position. wheel
U5 Tube available Detects when a specimen tube is in one of the Optical sensor Figure 2.8-1, carousel
tube slots of the carousel. assembly
S18 Door interlock Senses the front door is opened/closed. Magnetic Figure 2.8-1, on upper
sensor left corner of module
Figure 2.8-1 Rotary Cap-Pierce Module Components
B1, RCP Junction DS1/Q2, hand DS2/Q1, bar-code

carousel card detector LEDs detector LEDs
motor Carousel
assembly
LV24
Bar-code
reader
Needle
LV23
assembly
U2, needle
forward sensor
U1, needle
home sensor
Tray Tube
U2
ejector
U5, tube
available
CL5 CL4, CL5, needle-drive sensor U3, normal U4, oversized
U1 clamp cylinder position sensor position sensor
(configuration B) cylinder (configuration A) 5961285E
2.8-2 PN 4235961E
Summary of Operation
To initiate a cycle in the Rotary Cap-Pierce module, the operator begins at the DMS, selecting
the Primary mode of operation and the size of the specimen tube to be processed.
Note: The Rotary Cap-Pierce module can process two sizes of specimen tubes, normal and
oversized. The tube-size selection at the DMS determines to which position the carousel
assembly homes.
Next the operator places the specimen tube in front of the scanning window, with the
bar-code label facing the reader. The bar-code detector LEDs, DS2/Q1, detect the presence of
the tube and turn the bar-code reader on. After the bar-code label is read, a beeper sounds on
the Bar-Code Reader Decoder card and the green LED flashes for three to four seconds. The
operator must insert the tube into the carousel assembly before the green LED stops flashing.
When the operator inserts the specimen tube into the carousel assembly:
1. The specimen tube activates the tube available sensor, U5, indicating a specimen tube is
in a tube slot.
2. The red LED lights and remains on until the tube is ejected.
3. The instrument checks the condition of:
r The door interlock, S18, to ensure the lower door is closed.
r The needle home sensor, U1, to ensure the needle is retracted.
r The hand detector LEDs, DS1/Q2, to ensure nothing is blocking the rotation of the
carousel assembly.
4. The carousel motor, B1, rotates the carousel assembly to the piercing position.
5. The appropriate sensor on the code wheel, normal position, U3, or oversized position,
U4, indicates when to stop.
When the specimen tube is in the piercing position:
1. LV23 is energized, activating CL4 to clamp the tube in position.

2. LV24 is energized, activating CL3 to extend the needle, piercing the cap of the tube.
3. The instrument checks the needle forward sensor, U2, to ensure the needle is fully
extended.
4. The instrument aspirates a sample from the specimen tube.
After aspiration:
1. LV24 is de-energized and CL3 retracts the needle.

2. LV23 is de-energized and CL4 withdraws the clamp from the tube.
3. The instrument checks the blood/bubble detectors to ensure the aspiration was good. If
the blood/bubble detectors sense a partial aspiration, the instrument rinses the aspiration
pathway and repierces the specimen tube for a second aspiration.
After the instrument starts analyzing the sample, the carousel assembly rotates the specimen
tube to the tube ejector position where the tube ejector forces the tube out into the exit tray.
Then the carousel assembly rotates back to the home position, ready for the next
specimen tube.
PN 4235961E 2.8-3
RCP Junction Card

Function
The RCP Junction card is mounted on the front of the Rotary Cap-Pierce. See Figure 2.8-1
for location.
Either of two RCP Junction cards, the RCP Junction I or the RCP Junction II card can be used,
depending on the Sample Handler card in use. The RCP Junction I card must be used with the
Sample Handler I card, the RCP Junction II card with the Sample Handler II card.
The RCP Junction card interfaces the entire Rotary Cap-Pierce module to the Sampler
Handler card via a ribbon cable from J3/P16 on the RCP Junction card to P15/J15 on the
Sample Handler card. The RCP Junction card interfaces:
r Eight sensors:
t DS1/Q2, hand detector LEDs
t DS2/Q1, bar-code detector LEDs
t S18, door interlock
t U1, needle home
t U2, needle forward
t U3, normal position
t U4, oversized position
t U5, tube available
r The carousel stepper motor, B1
r Two solenoids:
t LV23, tube clamp
t LV24, needle cylinder
For details about these components, refer to Table 2.8-1, Rotary Cap-Pierce Module - Motors,
Solenoids, and Sensors/Switches.
2.8-4 PN 4235961E
RCP Junction Card Inputs/Outputs
Inputs: Outputs:
r Electronic Power Supply r Sample Handler card

r Sample Handler card r LV23, tube clamp
r Rotary Cap-Pierce module sensors: r LV24, needle cylinder
t DS1/Q2, hand detector r B1, carousel motor
t DS2/Q1, bar-code detector
t S18, door interlock
t U1, needle home
t U2, needle forward
t U3, normal position
t U4, oversized position
t U5, tube available
PN 4235961E 2.8-5
2.8-6 PN 4235961E
SAMPLE HANDLER SYSTEM - AUTOLOADER MODULE 2
2.9 SAMPLE HANDLER SYSTEM - AUTOLOADER MODULE
Function
The Autoloader module is a specimen-tube transport system with a closed vial, cap-piercing
mechanism, capable of transporting and processing up to 25 specimen tubes for introducing
samples into the instrument. For the location of the Autoloader module, see Figure 2.1-2.
Description
The Autoloader module consists of:
r A loading bay for holding the cassettes full of unprocessed specimen tubes. See
Figures 2.9-1 and 2.9-2.
r A rocker bed for transporting the cassettes of specimen tubes from the loading bay to the
piercing station and from the piercing station to the unloading bay, and for mixing
(rocking) the specimen tubes. See Figures 2.9-1 and 2.9-2.
r A bar-code reader for identifying the cassette, the tube position in the cassette, and the
specimen tubes (optional). See Figures 2.9-1 and 2.9-2.
r A needle assembly for piercing the specimen tubes for sample aspiration. See
Figures 2.9-1 and 2.9-2.
r An unloading bay for collecting the cassettes full of processed specimen tubes for
retrieval by the operator. See Figures 2.9-1 and 2.9-2.
r Motors, solenoids, cylinders, and sensors/switches for performing and monitoring the
functions of the Autoloader module.
All the motors (B = stepper motors, M = dc motors), solenoids (LV), and sensors/
switches (S) associated with the Autoloader module functions, except the blood/bubble
detectors, are listed in Table 2.9-1, Autoloader Module - Motors, Solenoids, and Sensors/
Switches. The locations of these components are shown in Figures 2.9-1 through 2.9-3.
For a description of the blood/bubble detectors, see Blood/Bubble Detectors under
Heading 2.11, SAMPLE PROCESSING SYSTEM - DILUTER.
r Two interface cards, the Rocker Bed Interface card for interfacing the components on the
rocker bed to the Autoloader Interface card, and the Autoloader Interface card for
interfacing the components in the Autoloader module with the Sample Handler card.
The Autoloader module is controlled via the Sample Handler card.
t For details about the interface cards, see Autoloader Interface Card and Rocker Bed
Interface Card at the end of this section.
t For details about the Sample Handler card, see Heading 2.7, SAMPLE HANDLER
SYSTEM - SAMPLE HANDLER CARD.
Table 2.9-1 Autoloader Module - Motors, Solenoids, and Sensors/ Switches
Item Functional Description Function Style Location

B1 Load elevator motor Raises and lowers the right elevator (lift) to Stepper motor Figure 2.9-2, on
place a cassette onto the rocker bed. rocker bed
B2 Unload elevator motor Raises and lowers the left elevator to remove Stepper motor Figure 2.9-2, on
a cassette from the rocker bed. rocker bed
PN 4235961E 2.9-1
SAMPLE HANDLER SYSTEM - AUTOLOADER MODULE
Table 2.9-1 Autoloader Module - Motors, Solenoids, and Sensors/ Switches (Continued)

LV81 Tube return Controls the tube return [into cassette] Solenoid Figure 2.9-3, on
cylinder, CL9. CL9 returns the specimen tube module housing
into the cassette after sampling.
LV82 Pierce needle Returns the tube ram cylinder, CL7, in Solenoid Figure 2.9-3, on
conjunction with LV83, and controls the Autoloader Interface
needle drive cylinder, CL6. card
Note: On the Autoloader Interface card, LV82
is labeled SW2.
LV83 Tube ram Controls the tube ram cylinder, CL7, in Solenoid Figure 2.9-3, on
conjunction with LV82. CL7 pushes the Autoloader Interface
specimen tube out of the cassette. card
Note: On the Autoloader Interface card, LV83
is labeled SW3.
LV84 Bed lock Controls the bed-lock cylinder, CL8. CL8 Solenoid Figure 2.9-3, on
locks the rocker bed in appropriate positions. module housing
M1 Rocker bed motor Controls rocking of the rocker bed. DC motor Figure 2.9-3, on
module housing
M2 Cassette index motor Drives the cassette index mechanism DC motor Figure 2.9-2, on
rocker bed
S0 Needle home Senses the needle is fully retracted to the Hall effect Figure 2.9-1, on CL6
home position. sensor or (Hall effect) or
Optical sensor Figure 2.9-2, at
Piercing station
(optical)
S1 Needle forward Senses the needle is forward in the piercing Hall effect Figure 2.9-1, on CL6
position. sensor or (Hall effect)
Optical sensor Figure 2.9-2, at
Piercing station
(optical)
S2 Cassette position 1 Senses a cassette has advanced the first time, Optical sensor Figure 2.9-1, on
cassette position 1. rocker bed
S3 Cassette position 2 Senses that a cassette is overlapping the Optical sensor Figure 2.9-1, on
elevator platform, cassette position 2. rocker bed
S4 STOP switch Immediately stop Autoloader mechanism. Switch On lower door
S5 Door interlock Senses the front door is opened/closed. Magnetic Figure 2.9-1, on
sensor module housing
S6 Unload elevator down Senses when the left elevator platform is all Optical sensor Figure 2.9-1, on
the way down. rocker bed
S7 Tube available Senses the presence of a specimen tube in Optical sensor Figure 2.9-1, on
the cassette at the piercing station. rocker bed
S8 Load elevator down Senses when the right elevator platform is all Optical sensor Figure 2.9-1, on
the way down. rocker bed
S9 Full cass index rotation Senses when the cassette indexing Optical Figure 2.9-1, on
mechanism is inactive. This sensor becomes Sensor rocker bed
not true when the cassette indexing
mechanism is advancing the cassette.
2.9-2 PN 4235961E
Table 2.9-1 Autoloader Module - Motors, Solenoids, and Sensors/ Switches (Continued)

S10 Tube ram Senses the tube ram cylinder is fully retracted Hall effect Figure 2.9-3, on tube
to the home position. sensor ram
S11 Unload stack full Senses the unloading bay is full - five Optical sensor Figure 2.9-1, on
cassettes are present. module housing
S12 Cassette position 0 Senses a cassette is placed on the rocker bed Optical sensor Figure 2.9-1, on
and is oriented correctly, cassette position 0. rocker bed
S13 Cassette position 3 Senses that a cassette is over the elevator Optical sensor Figure 2.9-1, on
platform, cassette position 3. rocker bed
S14 Tube forward Senses a specimen tube is present and Optical sensor Figure 2.9-2, at
pushed against the stop in the piercing Piercing station
station, ready for piercing.
S15 Load stack empty Senses the loading bay is empty. Optical sensor Figure 2.9-1, on
module housing
S16 Pierce position Senses when the rocker bed is in the forward, Optical sensor Figure 2.9-2, on
piercing position. module housing
S17 Horizontal position Senses when the rocker bed is in the level, Optical sensor Figure 2.9-2, on
cassette loading and unloading position. module housing
Figure 2.9-1 Autoloader Module Components, Front View, Rocker Bed Forward
S11, unload stack Bar-code reader

full sensor
Unloading bay Loading bay

ION INTO BEAM
CT
PRODU
II LASER
STARE
DO NOT
LASER UM CLASS
CAUT
DIODE MAXIM
LIGHT
ATT
1.0 MILLIW
LASER
670 nm
Rocker bed S15, load stack

empty sensor
S3, cassette
position 2 sensor S2, cassette
position 1 sensor
S13, cassette S12, cassette
position 3 sensor position 0 sensor
Unload Load elevator
elevator
S7, tube available
S6, unload elevator sensor
down sensor S8, load elevator
down sensor
S9, full cas index
rotation sensor Needle assembly
S5, door S1, needle

interlock forward sensor
M A N U A LS .
E R LY .R E FE R TO P R O D U C T
H A N D LE O R S TO R E N E E D LE
CL6, needle
S0, needle drive cyclinder

5961279E home sensor (configuration B)
PN 4235961E 2.9-3
Figure 2.9-2 Autoloader Module, Front View, Rocker Bed Backward
S11, unload stack Bar-code reader

full sensor
Loading bay
Unloading bay
CL7, tube ram Cassette shelf
cylinder
S15, load stack
Cassette shelf
empty sensor
Rocker Bed
Rocker bed Interface card
B2, unload B1, load elevator

elevator motor motor
M2, cassette S17, horizontal
index motor position sensor
(in front of CL8)
S16, pierce position
S1, needle
sensor (behind CL8)
forward sensor
CL8, bed-lock
cylinder
S0, needle S14, tube
home sensor CL6, needle- Needle
CL9, tube- drive cylinder assembly forward
return cylinder (configuration A) sensor
5961277E
Figure 2.9-3 Autoloader Module Components, Rear View
S10, tube ram

sensor
LV84
CL7, tube ram

cylinder
LV82
LV81
LV83
M1, rocker bed

motor
Autoloader
Interface card
5961278D
2.9-4 PN 4235961E
Summary of Operation
To process samples using the Autoloader module, the operator inserts the specimen tubes
into bar-code labeled cassettes (up to five tubes per cassette), and places the cassettes (up to
five) in the loading bay.
Placing a cassette on the cassette shelf in the loading bay activates the load stack empty
sensor, S15, indicating a cassette is available for cycling. To initiate a Primary-mode cycle, the
operator must both place a cassette in the loading bay to activate S15 and select the Primary
mode of operation at the DMS - in either order.
After the operator initiates the cycle, the instrument:
1. Checks the condition of:

r The door interlock, S5, to ensure the lower door is closed.
r The needle home sensor, S0, to ensure the needle is retracted.
2. Homes the rocker bed to the horizontal position and engages the bed lock.
r The rocker bed motor, M1, moves the rocker bed.
r One of two rocker bed position sensors, the horizontal position sensor, S17, senses
when the rocker bed is horizontal.
r The bed lock consists of a solenoid activated cylinder, CL8, and a lock wheel with
two notches, one for the horizontal position of the rocker bed, and the other for the
pierce position. To engage the bed lock, LV84 is energized, which extends CL8 into
one of the notches in the lock wheel.
After the rocker bed homes:
1. The right elevator ascends, pushing the bottom cassette in the loading bay above the
cassette shelf and pushing the cassette shelf rollers out of the way, into the side walls of
the loading bay. The load elevator stepper motor, B1, raises and lowers the right elevator.
2. The right elevator descends, lowering the bottom cassette to the rocker bed.
3. After the right elevator passes the cassette shelf, the cassette shelf rollers springs back
into position so that only one cassette is lowered onto the rocker bed.
After the cassette is lowered onto the rocker bed:
1. The instrument checks:

r The load elevator down sensor, S8, to ensure the elevator is all the way down.
r The cassette position 0 sensor, S12, to ensure the cassette is at the rocker bed and is
oriented correctly.
2. The cassette index motor, M2, drives the cassette index mechanism which moves two
index fingers in and out of the top of the rocker bed. The index finger nearest the loading
bay hooks onto one of the rails on the bottom of the cassette and advances the cassette
from right to left on the rocker bed.
3. The full cassette index rotation sensor, S9, monitors the rotation of the cassette index
mechanism; nine rotations advance a cassette from the loading bay to the unloading bay.
4. As the cassette advances, four cassette position sensors on the top rear edge of the rocker
bed monitor its progress.
PN 4235961E 2.9-5
5. When the cassette simultaneously activates the cassette position 0 sensor, S12, and the
cassette position 1 sensor, S2, the rocker bed starts rocking to mix the specimen tubes
and the cassette index mechanism advances the cassette to the Piercing station.
6. The cassette index mechanism continues to advance the cassette until the first specimen
tube in the cassette pushes down on the spring-loaded flag of the tube available sensor,
S7, indicating a tube is present in the Piercing station.
Note: The cassette position 1 sensor, S2, and cassette position 2 sensor, S3, in
conjunction with the tube available sensor, S7, monitor the advancement of the cassette
through the Piercing station.
At the Piercing station:
1. The cassette index mechanism stops advancing the cassette, but the rocker bed
continues to rock.
2. The bar-code reader scans the cassette/position label on the cassette and the specimen
tube label. If the bar-code reader is unable to read the cassette/position label, the
Autoloader module stops and the instrument generates an error.
Note: The scanner only turns ON if a specimen tube is detected at the Piercing station.
3. If the bar-code reader successfully reads the bar-code labels, the rocker bed is tilted
forward to the pierce position, and locked into position. The pierce position sensor, S16,
senses when the rocker bed is in the pierce position.
4. When the rocker bed is locked in the pierce position, LV83 is energized to extend the
tube-ram cylinder, CL7. CL7 pushes the specimen tube out of the cassette and holds it
against the dead plate at the Piercing station.
5. The tube forward sensor, S14, senses when the tube is in position for piercing.
6. LV82 is energized, activating the needle drive cylinder, CL6. CL6 extends the needle,
piercing the cap of the tube.
7. The instrument checks the needle forward sensor, S1, to ensure the needle is fully
extended, and then aspirates a sample from the specimen tube.
After aspiration:
1. LV82 is de-energized and CL6 retracts the needle. The needle home sensor, S0, senses
when the needle is fully retracted.
2. The instrument checks the blood/bubble detectors to ensure the aspiration was good. If
the blood/bubble detectors sense a partial aspiration, the instrument rinses the aspiration
pathway and repierces the specimen tube for a second aspiration.
3. LV83 is de-energized and CL7 retracts, releasing its hold on the specimen tube. The tube
ram sensor, S10, senses when the tube ram is fully retracted.
4. LV81 is energized, activating the tube return cylinder, CL9. CL9 operates a small arm
that pushes the specimen tube back into the cassette.
5. The rocker bed resumes rocking and the bar-code reader scans the cassette/position label
on the cassette and the specimen tube label a second time. If the bar-code readings do
not match the readings taken before aspiration, the Autoloader module stops and the
instrument generates an error.
2.9-6 PN 4235961E
6. If the bar-code readings taken before and after piercing are identical (a positive
patient ID), the rocker bed continues to rock and the cassette index mechanism resumes
operation, advancing the cassette until the tube available sensor detects the next
specimen tube.
Note: When the fourth tube position in a cassette reaches the Piercing station, if cassettes
remain in the loading bay, the next cassette is lowered onto the rocker bed.
When the cassette reaches the unloading bay:
1. The cassette simultaneously activates the cassette position 2 sensor, S3, and the cassette
position 3 sensor, S13, which stops and locks the rocker bed in the horizontal position
and activates the left elevator. The unload elevator stepper motor, B2, raises and lowers
the left elevator.
2. The left elevator ascends, lifting the cassette off the rocker bed, and pushing it above the
cassette shelf in the unloading bay.
Note: When the cassette reaches the cassette shelf in the unloading bay, it pushes the
cassette shelf rollers out of the way, into the side walls of the unloading bay.
3. The cassette shelf rollers spring back into position, holding the cassette in the unloading
bay for retrieval by the operator, and the left elevator descends.
4. When the load elevator down sensor, S6, senses the elevator is down, if cassettes remain
on the rocking bed, the rocker bed and cassette index mechanism resume operation.
5. The Autoloader module continues to process cassettes until the unloading bay is full and
the top cassette activates the unload stack full sensor, S11, or until all the cassettes in the
loading bay are processed, whichever comes first.
Autoloader Interface Card
Function
The Autoloader Interface card, mounted on the rear of the Autoloader module, interfaces the
entire Autoloader module to the Sampler Handler card via connector J9 and a 50-pin ribbon
cable in concert with the Rocker Bed Interface card.
The Autoloader Interface card directly interfaces:
r Ten of the 18 sensors associated with the Autoloader module.

t S0, needle-home sensor
t S1, needle-forward sensor
t S4, STOP switch
t S5, door-interlock switch
t S10, tube-ram sensor
t S11, unload-stack full sensor
t S14, tube-forward sensor
t S15, load-stack empty sensor
t S16, pierce-position sensor
t S17, horizontal-position sensor
PN 4235961E 2.9-7
r The rocker bed motor, M1, that drives the rocker bed rocking mechanism.
r The four solenoids on the Autoloader module.
t LV81 tube return
t LV82 pierce needle
t LV83 tube ram
t LV84 bed lock
Note: Of the solenoids interfaced by the Autoloader Interface card, LV82 and LV83 are
located directly on the card. LV84 and LV81 are located on the left rear.
For details about these components, refer to Table 2.9-1, Autoloader Module - Motors,
Solenoids, and Sensors/ Switches.
Autoloader Interface Card Inputs/Outputs
Inputs: Outputs:
r Electronic Power Supply r Sample Handler card

r Sample Handler card r Rocker Bed Interface card
r Rocker Bed Interface card r LV81 tube return
r Autoloader module sensors r LV82 pierce needle
t S0, needle-home sensor r LV83 tube ram
t S1, needle-forward sensor r LV84 bed lock
t S4, STOP switch r M1, rocker bed motor
t S5, door-interlock switch
t S10, tube-ram sensor
t S11, unload-stack full sensor
t S14, tube-forward sensor
t S15, load-stack empty sensor
t S16, pierce-position sensor
t S17, horizontal-position sensor
Rocker Bed Interface Card
Function
The Rocker Bed Interface card, located under the rocker bed, interfaces the following to the
Autoloader Interface card via connector J20 and a 30-pin Hi-flex ribbon cable.
r Eight out of the total 18 Autoloader module related sensors.

t S2, cassette position 1 sensor
t S6, unload elevator down sensor
t S7, tube available sensor
t S8, load elevator down sensor
2.9-8 PN 4235961E
t S9, full cass index rotation sensor
r Three of the four motors.
t B1, load (right) elevator motor
t B2, unload (left) elevator motor
t M2, cassette-index motor
For details about these components, refer to Table 2.9-1, Autoloader Module - Motors,
Solenoids, and Sensors/ Switches.
Rocker Bed Interface Card Inputs/Outputs
Inputs: Outputs:
r Electronic Power Supply r Autoloader Interface card

r Autoloader Interface card r B1, load (right) elevator motor
t Autoloader module sensors S2, r B2, unload (left) elevator motor
cassette position 1 sensor r M2, cassette-index motor
t S6, unload elevator down sensor
t S7, tube available sensor
t S8, load elevator down sensor
t S9, full cass index rotation sensor
PN 4235961E 2.9-9
2.9-10 PN 4235961E
SAMPLE HANDLER SYSTEM - AUTOSENSOR TEST CARD 2
2.10 SAMPLE HANDLER SYSTEM - AUTOSENSOR TEST CARD
The Autosensor Test card monitors the status of every sensor on the Autoloader or Rotary
Cap-Pierce module and lights LEDs to indicate the status. Figure 2.10-1 shows which LEDs
are lit when the instrument is turned on.
The Autosensor Test card is not a part of the instrument; it is a separate component connected
to the Sample Handler card via a ribbon cable. The part number is listed in Table 8.1-21,
Tools and Supplies.
Figure 2.10-1 Autosensor Test Card - LEDs Lit Following Power Up

J1 To Sample Handler card J15
J2 To Autoloader Interface card J8 U1 U2 U3

C1
or
To RCP Interface card J1
* *
AL AND RCP AL AND RCP
CR8 CR11 CR17 CR5

AL-PIERCE POS AL-STOP SWITCH
AL-TUBE AVAIL AL-TUBE RAM
RCP-HAND DETECT RCP-SPARE 04
(SWITCH 7) (SWITCH 10)
(SWITCH 16) (SWITCH 4)
* AL
CR15 CR1 CR2 CR6
AL-TUBE FWD AL-NEEDLE HOME AL-NEEDLE FWD AL-DOOR INTLX
(SWITCH 14) RCP-NEEDLE HOME RCP-NEEDLE FWD RCP-DOOR INTLX
(SWITCH 0) (SWITCH 1) (SWITCH 5)
* AL * AL * AL AND RCP
CR7 CR12 CR16 CR9 CR18
AL-UNLOAD AL-UNLOAD AL-LOAD STACK AL-LOAD AL-HORIZ POS
ELEVATOR DOWN STACK FULL EMPTY ELEVATOR DOWN RCP-BAR-CODE DET
(SWITCH 6) (SWITCH 11) (SWITCH 15) (SWITCH 8) (SWITCH 17)
CR14 CR4 CR3 CR13 CR10

AL-CASS POS 3 AL-CALL POS 2 AL-CASS POS 1
(SWITCH 13) RCP-OVS PIERCE RCP-NORM PIERCE AL-CASS POS 0 AL-FULL INDEX
(SWITCH 3) (SWITCH 2) (SWITCH 12) (SWITCH 9)
* LED turns on when condition is true = LED ON

AL = Autoloader module = LED OFF
RCP = Rotary Cap-Pierce module 5961165D
PN 4235961E 2.10-1
SAMPLE HANDLER SYSTEM - AUTOSENSOR TEST CARD
2.10-2 PN 4235961E
SAMPLE PROCESSING SYSTEM - DILUTER 2
2.11 SAMPLE PROCESSING SYSTEM - DILUTER
Function
The Diluter aspirates, pipets, dilutes, mixes, lyses and senses the blood sample in preparation
for analysis. To review these functions, read the Sample Flow section in Chapter 3 of the
Reference manual. To better understand how these functions are performed, read this section
and study the Pneumatic/Hydraulic Layout, DCN 6320510, in Chapter 6.
Description
The Diluter comprises six modules, accessible either from the front or the right-side of the
Main Unit:
r BSV (Sample Valve). For location, refer to Figure 2.1-1, Main Unit with Rotary
Cap-Pierce Module, Front View, Main Component Locations, or Figure 2.1-2, Main Unit
with Autoloader Module, Front View, Main Component Locations.
r CBC. For location, refer to Figure 2.1-1, Main Unit with Rotary Cap-Pierce Module,
Front View, Main Component Locations, or Figure 2.1-2, Main Unit with Autoloader
Module, Front View, Main Component Locations.
r Flow-Cell. For location, refer to Figure 2.1-4, Main Unit Right-Side View, Main
Component Locations.
r Main Diluter. For location, refer to Figure 2.1-4, Main Unit Right-Side View, Main
r Mixing Chamber. For location, refer to Figure 2.1-4, Main Unit Right-Side View, Main
r Pump. For location, refer to Figure 2.1-1, Main Unit with Rotary Cap-Pierce Module,
Front View, Main Component Locations, or Figure 2.1-2, Main Unit with Autoloader
Module, Front View, Main Component Locations.
The Diluter modules are interconnected with each other and with the Sample Handler
module (Rotary Cap-Pierce or Autoloader) via color-coded tubing through quick disconnects.
A label (Figure 2.11-1) on the inside of the Main Unit’s right-side door lists the color coding
for the instrument tubing.
Three of the Diluter modules, the BSV, CBC, and Pump modules, can be removed from the
front of the Diluter, or repositioned, to provide access for maintenance.
Main Components
The main components in the Diluter are identified in Figures A.5-1 through A.5-10 and, with
the exception of the solenoids and pinch valves, described in Table A.5-1, Diluter Component
Location References and Functions.
Solenoid Valves
The solenoids are mounted directly on pressure and vacuum manifolds in each respective
Diluter module. The DILUTER INTERFACE card controls all the solenoids except those
located on the Sample Handler modules which are controlled by the Sample Handler card.
A complete list of the solenoids and the devices they control can be found in Table 4.26-1,
Solenoid Operations, and inside the right-side door of the Main Unit (Figure 2.11-1).
PN 4235961E 2.11-1
SAMPLE PROCESSING SYSTEM - DILUTER
Figure 2.11-1 Solenoid and Tubing Label
SOLENOID NUMBERS / NAMES TUBING

CBC MODULE MAIN DILUTER MODULE FUNCTION COLOR CODE
MF 1 MF 10
PRESSURE [30 PSI] GREEN/CLEAR
01 CBC WASTE-PRESSURE 35 WBC/RBC DISPENSER
02 RBC COUNT 36 CBC HIVAC PRESSURE [30 PSI INTERRUPTED] GREEN/BLACK/CLEAR
03 BATH DRAIN 37 BLEACH ASP
04 CBC LYSE 38 BLEACH MIX CHAMBER SHEATH PRESSURE [6 PSI] GRAY/CLEAR
05 WBC COUNT 39 BLEACH ISOLATOR
06 BLEACH BATH 40 PROBE VAC SAMPLE PRESSURE [7 PSI] GRAY/BLACK/CLEAR
41 BSV CLEAN
42 DETERGENT VENT / AIR PUMP BLACK/CLEAR
43 DIFF WASTE PRESSURE
MF 8 44 ERYTHROLYSE-1 PRESSURE [6 PSI INTERRUPTED] BLACK
45 DIFF WASTE VAC
07 WBC BUBBLE MIX 46 BACKWASH HIGH VACUUM YELLOW/CLEAR
08 RBC BUBBLE MIX 47 CBC WASTE DRAIN
48 CLEANER LOW VACUUM YELLOW/BLACK/CLEAR
49 SAMPLE PRESSURE
50 FLUSH UPPER HIGH VAC/LOW VAC ORANGE/CLEAR
SAMPLE VALVE MODULE 51 RUN
52 EXIT SAMPLE HIGH VAC/LOW VAC [30 PSI] ORANGE/BLACK/CLEAR
MF 3 67 SHEATH REFILL
14 AIR PUMP 68 COMPRESSOR BLEED HIGH VAC [30 PSI] RED/CLEAR
15 BUBBLE MAKE
20 PROBE CLEAN HIGH VAC/VENT [6 PSI] RED/BLACK/CLEAR
21 SECOND ASP
22 PRE-PREP ASP DILUENT WHITE/CLEAR
53 BUBBLE MAKE CLAMP
CLEANER CLEAR
MF 2 70 WATER TRAP DISINFECTANT CLEAR

16 PROBE ROTATE CLEANER/DISINFECTANT/DILUENT VIOLET/CLEAR
17 PROBE RETURN MIXING CHAMBER MODULE
18 RETURN VALVE CBC LYSE BROWN/CLEAR
19 SEGMENT MF 15
PAK LYSE E.V.A. TUBING
31 PRE-PREP MIX CHAMBER
32 RINSE MIX CHAMBER PAK QUENCH STABILIZE E.V.A. TUBING
PUMP MODULE 33 DRAIN MIX CHAMBER
MF 5 34 ERYTHROLYSE DISABLE WASTE/BIOHAZARD CLEAR
54 FLUSH LOWER
25 55 EXIT UPPER
ASPIRATE PIERCER 56
26 ASPIRATE PROBE STABILYSE
27 57 SAMPLE DELIVER
ERYTHROLYSE 58
28 NEEDLE RINSE EXIT LOWER
29 59 STABILYSE ENABLE
BELLOWS DRAIN
30 NEEDLE AIR DRY
65 MIX MOTOR
PIERCER MODULE AUTOLOADER MODULE NOTES:

MF 16
1. MF = MANIFOLD
23 CLAMP TUBE 84 BED LOCK
2. = DC MOTOR
81 TUBE RETURN
24 PIERCE NEEDLE 82 PIERCE NEEDLE 83 CLAMP TUBE
5961015D
Blood Sampling Valve (BSV)

Blood is aspirated through the BSV, either directly through the aspirator tip in the Secondary
mode or indirectly through the piercing needle and front blood/bubble detector in the
Primary mode.
The BSV is self-cleaning and consists of three sections (also referred to as “pads”): left, center,
and right. The left section rotates, for Primary- and Secondary-mode changes, to direct the
blood sample, diluent, and rinse liquids to the appropriate ports in the valve. The center
section rotates to segment the blood samples (1.6-µL RBC, 28-µL WBC, and 28-µL diff) into
accurately measured amounts. The right section is fixed in position and does not rotate.
An illustration of the BSV sections (Figure 2.11-2) is affixed to the front panel of the
Electronic Power Supply. Figure 2.11-3 further illustrates the BSV’s flow paths and
wire markers.
2.11-2 PN 4235961E
Figure 2.11-2 BSV Ports and Wire Markers
WM 12 WM 11
WM 1 WM 2 WM 6 ASP/BACKWASH TO MIXING
SELF-CLEAN RBC PUMP PAK LYSE CHAMBER
WM 13
GROOVE (PIERCE) AIR PUMP WM 10
SELF-CLEAN
WM 7 GROOVE
WBC PUMP
WM 3
SAMPLE IN
(PIERCE)
WM 16
WM 5 WM 4 WM 9 WM 8 WM 14
RBC PUMP
SELF-CLEAN RBC PUMP TO WBC WBC PUMP TO RBC BATH
(X-RINSE)
GROOVE (PROBE) BATH (X-RINSE) WM 15
SELF-CLEAN GROOVE
LEFT SECTION (PAD) CENTER SECTION (PAD) RIGHT SECTION (PAD)

5961172C
Figure 2.11-3 BSV Flow Paths and Wire Markers

LEFT SECTION (PAD) CENTER SECTION (PAD) RIGHT SECTION (PAD)
WM 12
WM 6 WM 11
WM 13
WM 1 WM 10
WM 7
WM 14
WM 2
WM 4 WM 16
WM 5
WM 3
WM 15
WM 8
WM 9
SILVER
ALIGNMENT
BAR
5961198C
Blood/Bubble Detectors
In the Primary mode, two optical sensors, BD1 and BD2, monitor aspiration of the blood
sample through the BSV. See Figure A.5-1, BSV Module, Old Configuration, Front View, or
Figure A.5-2, BSV Module, New Configuration, Front View, for the location of the
blood/bubble detectors. The instrument checks the blood/bubble detectors six times during
aspiration to verify good aspiration.
1. Diluent comparison (front and rear blood/bubble detectors) - At the initiation of
aspiration, the instrument checks for diluent (>70% transmittance) at both blood/bubble
detectors and compares the readings to ensure the readings are within ±4% of each other.
If this check fails, the cycle is immediately stopped and the instrument generates a
DILUENT COMPARISON OUT OF LIMITS error message.
Note: This is the only blood/bubble detector check that causes the cycle to
immediately stop.
PN 4235961E 2.11-3
2. Air check (front blood/bubble detector) - Next the instrument checks the front
blood/bubble detector for the air gap (30% - 50% transmittance). When the air gap is
passing the front blood/bubble detector, the rear blood/bubble detector should still be
detecting diluent.
3. Blood check (front blood/bubble detector) - After the air gap passes, the instrument
checks the front blood/bubble detector for blood (<20% transmittance). This value is
stored. The rear blood/bubble detector should still be detecting diluent.
Note: The front blood/bubble detector should detect blood for the remainder of
aspiration.
4. Air check (rear blood/bubble detector) - Next the instrument checks the rear
blood/bubble detector for the air gap (30% - 50% transmittance). After the air gap passes
the rear blood/bubble detector, the instrument removes the high vacuum from the
aspiration pathway.
5. Blood check (rear blood/bubble detector) - After the air gap passes, the instrument
checks the rear blood/bubble detector for blood (<20% transmittance) and verifies the
blood reached the rear blood/bubble detector within 6.5 seconds. This value is stored.
6. Blood check (front and rear blood/bubble detectors) - At the end of aspiration (the blood
stops just beyond the rear blood/bubble detector), the instrument checks for blood
(<20% transmittance) at both blood/bubble detectors and compares the values stored in
steps 3 and 5 to ensure the values are within ±4% of each other.
If the aspiration fails the checks in steps 2, 3, 5, or 6 fail, the instrument backwashes and
pierces the specimen tube a second time. If the second aspiration fails, the cycle is completed
and the instrument generates a PARTIAL ASPIRATION error message.
The gains for the blood/bubble detectors can be measured and adjusted on the Sample
Handler card. See Heading 4.21, BLOOD/BUBBLE DETECTORS GAIN ADJUSTMENT.
Sample Processing
Primary-Mode Aspiration
Operation begins with both the front and center sections of the BSV rotated fully to the
clockwise position (with respect to the BSV knob) and pressing against the underside of the
silver alignment bar. Refer to Figure 2.11-3.
The 185-µL Primary-mode aspiration pump connects to the right section of the BSV, via the
rear blood/bubble detector, at wire marker 12 (WM12). See Figure 2.11-4. The sample line
from the piercing needle, through the front blood/bubble detector, connects to the left section
of the BSV at WM3.
When the 185-µL Primary-mode aspiration pump is actuated, whole blood is aspirated from
the piercing needle toward the front blood/bubble detector, but not directly into the BSV.
When the piercing needle retracts from the tube, the aspirated aliquot of blood (185 µL) is
pulled by vacuum (applied to the aspiration system by VL10) into fitting WM3 on the BSV.
The blood is pulled through the BSV, filling the 1.6-µL RBC segment in the BSV center
section, the 28-µL WBC loop in the right section, and the 28-µL diff segment loop in the
center section. The blood then continues out the right section at WM12 and through the rear
blood/bubble detector toward the 185-µL Primary-mode aspiration pump.
2.11-4 PN 4235961E
Figure 2.11-4 Primary-Mode Aspiration
28 µL
DIFF LOOP
TO 185 µL
WM 12 ASPIRATION
REAR PUMP
DETECTOR
1.6 µL RBC TO 3 mL
SEGMENT BACKWASH
PUMP
NEEDLE 28 µL WBC LOOP

FRONT WM 3
DETECTOR
5961057C
The only portions of the aspirated blood used for testing are those contained in the
1.6-µL RBC segment in the center section of the BSV and the two 28-µL loops, WBC and diff.
WBC/RBC Sample Delivery

When blood is detected by both the front and rear blood/bubble detectors, the BSV center
section rotates counterclockwise until it presses against the top side of the silver alignment
bar (Figure 2.11-3) to segment off the blood samples contained in the 1.6-µL RBC segment in
the center section of the BSV and the two 28-µL loops, WBC and diff. See Figure 2.11-5.
Figure 2.11-5 WBC/RBC Sample Delivery

FROM WBC
DILUENT DISPENSER
FROM RBC
WM 7
DILUENT
DISPENSER
1.6 µL WM 14
WM 2 TO RBC
RBC SEGMENT BATH
28 µL WBC LOOP
TO WBC WM 9
BATH 5961058C
The RBC diluent dispenser connects to the left section of the BSV at fitting WM2. The output
to the RBC bath connects to fitting WM14 on the right section of the BSV. The RBC diluent
dispenser delivers 10 mL of diluent into WM2, through the 1.6-µL RBC segment in the center
section, and outputs the diluent and blood, via WM14, to the RBC bath.
The WBC diluent dispenser connects to the center section of the BSV at fitting WM7. The
output to the WBC bath connects to fitting WM9 on the center section. The WBC diluent
dispenser delivers 6.0 mL of diluent into WM7, through the 28-µL WBC loop in the right
section, and outputs the diluent and blood, via WM9, to the WBC bath.
PN 4235961E 2.11-5
Diff Segmentation
When the BSV rotates to segment the RBC and WBC dilution segments, it also segments off
the sample in the 28-µL diff loop.
The 64-µL air pump connects to the BSV right section at fitting WM13. See Figure 2.11-6.
The input line to the mixing chamber connects to the BSV right section at fitting WM11. The
input line to the mixing chamber is left primed with Erythrolyse II reagent from the
previous cycle.
Figure 2.11-6 Diff Segmentation

28 µL
DIFF LOOP
TO MIXING
CHAMBER
WM 11 FITTING #3
WM 13 FROM 64 µL
AIR PUMP
5961059C
When the 64-µL air pump operates, air enters WM13 pushing the 28 µL of segmented blood,
followed by about 6.35 mm (0.25 in.) of air, out of the loop and into the input line to the
mixing chamber via WM11. The displacement of Erythrolyse II reagent in the mixing
chamber input line [28 µL of blood plus 6.35 mm (0.25 in.) of air] forces about 64 µL of
Erythrolyse II reagent into the mixing chamber through fitting number 3 of the mixing
chamber.
Diff Segment and Erythrolyse II Reagent Delivery

The BSV center section rotates back to the home position pressing against the underside of
the silver alignment bar. See Figure 2.11-3.
The 300-µL (total volume) Erythrolyse II reagent pumps are connected via the Peltier module
to the BSV center section at fitting WM6 (Figure 2.11-7). The mixing chamber input line is
connected to the BSV right section at fitting WM11. The mixing chamber input line contains
the 28-µL diff segment, Erythrolyse II reagent, and about 6.35 mm (0.25 in.) of air.
Figure 2.11-7 Diff Segment and Erythrolyse II Reagent Delivery to Mixing Chamber
FROM PAK
LYSE VIA
PELTIER
MODULE WM 6
AIR 28 µL
GAP OF BLOOD
TO MIXING
WM 11 CHAMBER
FITTING #3
5961060C
2.11-6 PN 4235961E
When the Erythrolyse II reagent pumps operate, Erythrolyse II reagent is delivered via the
Peltier module into the BSV center section at WM6, and is routed through the BSV to pick up
the air and 28-µL segment of blood and deliver them to the mixing chamber. The mixing
chamber input line is left primed with Erythrolyse II reagent for the next cycle. In addition,
133 µL of StabiLyse is delivered to the mixing chamber (via fitting #1 on the side of the
chamber) where the it is mixed with the blood, Erythrolyse II reagent dilution.
Cross Rinse to the Baths

For a cross rinse to the baths, the BSV center section is in the home position, pressing against
the underside of the silver alignment bar. See Figure 2.11-3.
The RBC diluent dispenser is connected to the BSV right section at fitting WM16
(Figure 2.11-8). The WBC bath input line connects to the BSV center section at WM9. The
RBC diluent dispenser delivers 10 mL of diluent to fitting WM16, through the BSV center
section, exiting to the WBC bath via fitting WM 9.
Figure 2.11-8 Cross Rinse to the Baths

From WBC
diluent
dispenser
WM14
To RBC bath
From RBC
WM8 diluent
dispenser
WM16
WM9
To WBC 5961061C
bath
The WBC diluent dispenser is connected to the BSV center section at fitting WM8
(Figure 2.11-8). The RBC bath input line connects to the BSV right section at WM14. The
WBC diluent dispenser delivers 6 mL of diluent to fitting WM8, through the BSV right
section, exiting to the RBC bath via fitting WM14.
Secondary-Mode Aspiration
For Secondary-mode aspiration, the BSV left section rotates counterclockwise with respect to
the BSV knob, pressing against the top of the silver alignment bar. The BSV center section is
clockwise (home position), pressing against the underside of the silver alignment bar. See
Figure 2.11-3.
PN 4235961E 2.11-7
The 125-µL Secondary-mode aspiration pump connects to the BSV right section, via the rear
blood/bubble detector, at fitting WM12 (see Figure 2.11-9). When the pump is activated,
whole blood is aspirated into the BSV left section via the whole blood aspirator tip, through
the 1.6-µL RBC segment in the center section, through the 28-µL WBC loop in the right
section, back through the 28-µL diff segment loop in the center section, and continues out
the right section through fitting WM12 and the rear blood/bubble detector toward the 125-µL
aspiration pump.
Figure 2.11-9 Secondary-Mode Aspiration

REAR BLOOD
28 µL
DIFF LOOP DETECTOR
TO 125 µL
ASPIRATION
PUMP
WM 12
1.6 µL RBC
SEGMENT
28 µL WBC LOOP
T
I
P
5961062C
The BSV center section then rotates clockwise to segment off two 28-µL segment and one
1.6-µL segment blood samples for the sample dilutions. The methods of dilution remain the
same as performed in the Primary mode with the exception of the RBC diluent and
sample delivery.
Note: Blood/bubble detectors are not active during Secondary-mode aspiration.
Pre-Prep/Latex Aspiration
For pre-prep samples or latex aspiration, the BSV left section rotates counterclockwise with
respect to the BSV knob, pressing against the top of the silver alignment bar. The BSV center
section is clockwise (home position), pressing against the underside of the silver alignment
bar. Refer to Figure 2.11-3.
High vacuum from the mixing chamber and VC7 is applied directly to the BSV right section
via the rear blood/bubble detector at fitting WM12. See Figure 2.11-10.
When high vacuum is directed to the BSV, pre-prep reticulocyte or latex samples are aspirated
into the BSV left section via the aspirator tip, through the 1.6-µL RBC segment in the center
section, through the 28-µL WBC loop in the right section, back through the 28-µL diff
segment loop in the center section, and continue out the right section through fitting WM12
and the rear blood/bubble detector, eventually ending up in the mixing chamber.
2.11-8 PN 4235961E
Figure 2.11-10 Pre-Prep Latex Aspiration
REAR BLOOD
28 µL
DETECTOR
DIFF LOOP
TO MIXING
CHAMBER
WM 12
1.6 µL RBC
SEGMENT
28 µL WBC LOOP
T
I
P
5961109C
Aspiration is performed in three subcycles:
1. Aspiration (through the BSV to the rear blood/bubble detector).

2. Transport and position (transport sample through the 0.030 in. i.d. pre-prep lines with
the first part of the sample to the mixing chamber, then drains).
3. Transport and deliver (the rest of the sample to the mixing chamber).
RBC Sample Delivery, Secondary Mode

The BSV center section rotates to the segment position, pressing against the top of the silver
alignment bar. Refer to Figure 2.11-3.
The RBC diluent dispenser is connected to the BSV left section at fitting WM 4 (see
Figure 2.11-11). The RBC bath input line connects to the BSV right section at fitting WM14.
The RBC diluent dispenser delivers 10 mL of diluent into fitting WM 4, through the 1.6-µL
RBC segment in the center section, and out to the RBC bath through fitting WM14.
Figure 2.11-11 Sample Delivery, Secondary-Mode
TO
RBC
WM 4 BATH
WM 14
1.6 µL RBC
SEGMENT
FROM RBC
DILUENT 5961063C
DISPENSER
PN 4235961E 2.11-9
Backwash Functions
The 3-mL backwash pump routes diluent through the entire aspiration path of both the
Primary and Secondary modes. The diluent enters the BSV right section at fitting WM12 for
both modes. The backwash liquid is collected in the needle bellows in the Primary mode or
by the rinse block in the Secondary mode, depending on the position of the left section of the
BSV. See Figure 2.11-12.
Figure 2.11-12 Backwash Functions
FROM 3 mL FROM 3 mL
28 µL BACKWASH 28 µL BACKWASH
DIFF LOOP PUMP DIFF LOOP WM 12 PUMP
WM 12
TO 125 µL
ASPIRATION
1.6 µL TO 185 µL 1.6 µL PUMP
RBC ASPIRATION RBC
SEGMENT PUMP SEGMENT
28 µL WBC LOOP 28 µL WBC LOOP

T
WM 3 I
NEEDLE P
PRIMARY MODE SECONDARY MODE 5961064C
2.11-10 PN 4235961E
SAMPLE ANALYSIS SYSTEM - CBC TECHNOLOGY 2
2.12 SAMPLE ANALYSIS SYSTEM - CBC TECHNOLOGY
Summary
The MAXM analyzer uses the Coulter Principle to count and measure the RBCs and Plts in
the RBC bath and the WBCs in the WBC bath, and measures Hgb photometrically in the WBC
bath. Electronic cards in the Analyzer measure and count the pulses produced by the cells,
and develop histograms for a graphical representation of size versus number. The remaining
CBC parameters (Hct, MCH, MCHC, RDW, Pct, and PDW) are calculated from the directly
measured parameters. See Chapter 3 in the customer’s Reference manual for a description of
the methods used to obtain the CBC parameters.
Applying the Coulter Principle
Sensing Area
Both aperture module and bath assemblies house an external electrode, an aperture, and an
internal electrode for applying aperture current across the aperture. See Figure 2.12-1.
Figure 2.12-1 Applying the Coulter Principle
Aperture
current
Vacuum
Internal
electrode
Aperture
module
and bath
assembly
Aperture
Sweep-flow line
(RBC aperture only)
External
electrode
5961220D
Aperture Current
Coaxial cables connect the internal electrodes to the R/W PREAMP card, which supplies the
aperture current to the apertures.
PN 4235961E 2.12-1
SAMPLE ANALYSIS SYSTEM - CBC TECHNOLOGY
Aperture Vacuum
Regulated low vacuum from the vacuum isolator chambers pulls the dilutions from the baths
through the apertures for sensing. In the RBC aperture module and bath assembly, the
vacuum also pulls diluent from the sweep-flow line, up behind the RBC aperture, to prevent
RBCs from swirling back into the sensing area and being recounted as platelets.
The low vacuum is regulated by the low vacuum regulator, RG1. Refer to Table A.1-13,
Pressure and Vacuum Tolerances, for the low vacuum specifications.
Each dilution is counted three times to obtain the final results. For details on the counting
times and sequences, see R/W/P PROC Card under Heading 2.4, ELECTRONIC SYSTEM -
ANALYZER MODULE.
Analyzing the RBC, WBC, and Plt Data

See R/W PREAMP Card and R/W/P PROC Card under Heading 2.4, ELECTRONIC SYSTEM -
ANALYZER MODULE, for a description of the cards used to analyze the RBC, WBC, and
Plt data.
Determining the Hgb

When the CBC lytic reagent lyses the RBCs in the WBC bath, it releases the hemoglobin. The
hemoglobin reacts with the CBC lytic reagent to form cyanmethemoglobin, a stable pigment.
To do the Hgb analysis the instrument takes two Hgb readings, one on clean diluent in the
WBC bath for the blank (provided by the cross rinse from the RBC diluent dispenser in the
previous cycle), and the other on the WBC dilution.
Incandescent light from the Hgb lamp passes through the WBC bath and then through a filter
with a transmission wavelength of 525 nm. A light sensitive diode generates current from the
transmitted light, which is converted to a voltage at the op-amp in the Hgb preamp. The
higher the Hgb concentration, the darker the dilution, the less light that can pass through the
dilution, and the lower the Hgb voltage.
The Hgb preamp routes the voltage to the I/O card where the analog output from the Hgb
preamp is converted to a digital value.
2.12-2 PN 4235961E
SAMPLE ANALYSIS SYSTEM - VCS TECHNOLOGY 2
2.13 SAMPLE ANALYSIS SYSTEM - VCS TECHNOLOGY
Summary
The MAXM analyzer uses the VCS technology to sense and analyze the WBC differential, and
on instruments with the Retics option, the reticulocyte population. See Chapter 3 in the
customer’s Reference manual for a description of the methods used to obtain the WBC
differential and reticulocyte parameters.
This section briefly describes the VCS Measurements taken by the MAXM analyzer to
determine the WBC differential (and the reticulocyte population, if applicable) and briefly
describes the two circuit cards used in sensing the VCS parameters, the RF Detector Preamp
Card, and the LS Preamp 5 Module. For a description of the circuit card used to analyze the
VCS measurements, see VCS PROCESSOR Card under Heading 2.4, ELECTRONIC SYSTEM
- ANALYZER MODULE.
VCS Measurements
To obtain the WBC differential or reticulocyte data, the MAXM analyzer simultaneously
measures three parameters on each cell: volume (DC), conductivity (RF) and light scatter
(LS); and derives three additional parameters, opacity (OP), rotated light scatter (RLS) and
linear light scatter (LLS).
V - Volume (DC)
Volume is measured using the Coulter Principle. Direct current (DC) is applied to two
electrodes to measure the impedance across the aperture of the flow cell. As a cell passes
through the aperture, the impedance increases, generating a pulse that is directly proportional
to the cell volume.
C - Conductivity (RF)
Conductivity is measured by applying a radio-frequency current (RF) to the electrodes.
Unlike the DC (low-frequency) current which flows around the cells, this high frequency
current flows through the cells. This measurement is a function of the cell volume and
internal conductivity, which is related to the physical and chemical contents of the cell.
S - Light Scatter (LS)

Light scatter is measured by passing the cells through a point focused laser beam. As a cell
passes through the laser beam in the flow cell, the cell scatters or reflects the light. The
scattered light is collected at angles between 10 degrees and 70 degrees, the range known as
median angle light scatter (MALS). The light is converted into a voltage pulse proportional to
the total amount scattered. These pulses define the shape and surface characteristics of
each cell.
PN 4235961E 2.13-1
SAMPLE ANALYSIS SYSTEM - VCS TECHNOLOGY
Opacity (OP)
Opacity is a transformation of the data derived from the ratio of the RF and DC components
obtained during data acquisition. It is calculated for every individual cell measurement or
event. Opacity has the effect of removing the size component, yielding a measurement that is
more closely related to the internal contents of the cell.
RF
OP ≈ --------
DC
Rotated Light Scatter (RLS)

Rotated light scatter is a transformation of the data derived from the ratio of the log of LS to
the DC pulse peak information. It has the effect of removing the size component, yielding a
measurement that is more closely related to the internal structure of the cell. RLS is used in
the analysis of the diff parameters.
Log ( LS )
10
RLS ≈ ----------------------------
DC
Linear Light Scatter (LLS)

Linear light scatter is a transformation of the data derived from a ratio of the LS and DC
components obtained during data acquisition. It has the effect of removing the size
component, yielding a measurement that is more closely related to the internal structure of
the cell. LLS is used in the analysis of the retic parameters.
LS
LLS ≈ --------
DC
Applying the VCS Technology

The components for sensing the VCS parameters are located in the Flow-Cell module
(commonly referred to as the Triple Transducer module or the TTM). Refer to Figure 2.1-4
for the location of the TTM, refer to Figure 2.13-1 for the location of the components within
the TTM.
Sensing Area
The flow cell in the TTM contains two electrodes and the aperture where the DC, RF, and LS
parameters are sensed for each cell. See Figure 2.13-1.
DC and RF Currents
The electrodes are connected to the RF Detector Preamp card (Figure 2.13-1) which supplies
the DC and the RF currents via a coaxial cable.
Laser Light Source

The TTM also houses the laser, a lens block, and a light scatter sensor. See Figure 2.13-1.
2.13-2 PN 4235961E
Figure 2.13-1 Applying the VCS Technology
RF Detector
Preamp card
Laser
LS Preamp module
Lens block
(-) Electrode
LS sensor Aperture
(+) Electrode
7535026A
Light from the laser shines through the lens block which focuses the light onto the aperture
in the flow cell. See Figure 2.13-2. The light scatter (LS) sensor collects the scattered light
from the flow cell.
Figure 2.13-2 Light Scatter Path
Lens block
LS sensor
Laser beam
Lens
Flow cell
5961284D
5915281H
Flow-Cell Hydraulics
Sheath fluid from the sheath tank flows through the flow cell at a fixed pressure to
hydrodynamically focus the sample stream through the center of the aperture, one cell at a
time. See Figure 2.13-3.
PN 4235961E 2.13-3
Figure 2.13-3 Hydrodynamically Focused Cells
FLOW CELL
SHEATH-FLUID IN
SAMPLE DILUTION
SENSING
AREA SHEATH FLUID
SHEATH-FLUID IN
SAMPLE IN 7253191A
Sample pressure, applied to the mixing chamber, pushes the dilution from the mixing
chamber into the sheath fluid stream in the flow cell for sensing. The sample pressure is
regulated higher than the sheath pressure to overcome the higher resistance of the
sample path.
The sheath fluid pressure is regulated by the sheath pressure regulator, RG3; the sample
pressure by the sample pressure regulator, RG4. Refer to Table A.1-13, Pressure and Vacuum
Tolerances, for the pressure specifications for the sheath pressure and the diff pressure (the
difference between the sheath and sample pressures).
Between samples, the flow cell is rinsed with diluent. See Figure 2.13-4 for ports used and
their function during sample flow and rinsing.
Figure 2.13-4 Flow Cell Hydraulics

SAMPLE MODE RINSE MODE
PORT 1
PORT 2
PORT CLOSED
PORT 3 PORT 3
PORT CLOSED
APERTURE
APERTURE PORT 4
PORT 5
PORT 5
7253269A
PORT 6
2.13-4 PN 4235961E
Analyzing the Data
As a sample passes through the aperture of the flow cell, each cell simultaneously:
r Impedes the DC current flow, yielding DC data.

r Impedes the RF current flow, yielding RF data.
r Scatters the laser light, yielding LS data.
See VCS PROCESSOR Card under Heading 2.4, ELECTRONIC SYSTEM - ANALYZER
MODULE, for a description of the card used to analyze the data.
Preprocessing
A unique feature of the MAXM analyzer is preprocessing. Preprocessing removes invalid
volume, conductivity, or scatter pulses during accumulation. As a result, MAXM analyzer
scatterplots have a higher number of points for evaluation and fewer points in the debris area.
When you activate the Service Disk, you automatically deactivate preprocessing. This allows
you to more easily troubleshoot diff or chemistry-related problems. It is important that you
use the Service Disk. See Heading 4.2, USING THE SERVICE DISK.
RF Detector Preamp Card

The RF Detector Preamp card is connected directly to the flow cell in the TTM module and is
calibrated as a unit with the laser and the flow cell. See Figure 2.13-1 for location.
Function
Figure 2.13-5 illustrates the signal flow within the RF Detector Preamp card.
The RF Detector Preamp card:
r Generates the DC and RF current applied to the flow cell.

r Separates and amplifies the DC and RF signals through its circuitry, and routes these
signals to the VCS PROCESSOR card.
r Checks the RF oscillator operation by means of the RF Detect circuit. The RF Detect
circuit sends the RF DETECT signal to the DILUTER INTERFACE card.
r Checks for a clogged flow cell and informs the DILUTER INTERFACE card if a clog
exists.
PN 4235961E 2.13-5
Figure 2.13-5 RF Detector Preamp Card Signal Flow
DC DC
J73-5,6
Preamp
Flow
cell
J67 RF
Generator RF RF
J73-3,4
300 VDC circuit Preamp
P69-1 C1
6.3 VDC
P69-3
RF RF DETECT
TP1 J104-1,2
Detect
(WHT)
DILUENT
Conductivity CLOG
TP3 DETECT
Voltage
TP2 Comparator J104-3,4

R44 (GRY)
Clog
Detect
Threshold TP4
Address (BLK)
5961223D
RF Detector Preamp Card Inputs/Outputs
Inputs: Outputs:
r RF Power Supply r Flow cell

r Flow cell r VCS PROCESSOR card
r DILUTER INTERFACE card r DILUTER INTERFACE card
LS Preamp 5 Module
Primary Function
The primary function of the LS Preamp 5 module is to convert the current signal from the
scatter sensor to voltage and amplify it to a level usable by the VCS PROCESSOR card. See
Figure 2.13-6.
2.13-6 PN 4235961E
Figure 2.13-6 LS Preamp 5 Module Block Diagram
J66 J113
1 +15 VDC LS OFFSET

1 TO I/O
EPS 2 -15 VDC OUT CARD
2
3 GND
GND
J62
REGION 1
1 LS CURRENT
GND DIFF
LS 2 TO
SENSOR J78
SENSOR 3 VOLTAGE AMP
SELECT LS OUT TO
4 CONVERTER 1
REGION 2 DIFF/RETIC GND VCS
2
GAIN SHIELD 3 PROCESSOR
J109 CARD
SELECT
+5 VDC
1 TP1 TP3 RETIC
RETIC DIFF/RETIC GAIN LS OFFSET AMP
INTERFACE SELECT
2
3
DIFF/RETIC SELECT 5961162D
Mode Select
Depending on which mode is selected, Diff or Retic, the output from signals from the scatter
sensor requires different amplification to provide appropriate signals to the VCS PROCESSOR
card. To accomplish this there are separate amplifiers for the Diff and the Retic modes. The
Diff/Retic Gain Select circuitry selects the appropriate amplifier and sends the LS OUT signal
to the VCS PROCESSOR card.
The scatter sensor has three regions which are isolated from each other. The Retic mode uses
Region 1. The Diff mode uses Regions 1 and 2. Region 3 is not used. Depending upon the
mode selected, the LS Sensor Select circuitry selects the appropriate region. See Figure 2.13-7.
Figure 2.13-7 LS Sensor Regions

3
2 1
5961194C
LS Offset Voltage
LS offset voltage is measured across TP1 and TP3 on the LS Preamp module. The ground or
negative input to the voltage meter being used must be connected to TP3. The voltage at
these test points is directly proportional to the dark/light current.
PN 4235961E 2.13-7
The LS offset voltage is routed from the LS Preamp module, connector J113, to the I/O card,
via the analog backplane. The I/O card monitors the LS offset voltage; the DMS displays the
voltage value during a System Test.
LS Preamp 5 Module Inputs/Outputs
Inputs: Outputs:
r -15 Vdc from Electronic Power Supply r I/O card

r +15 Vdc from Electronic Power Supply r VCS PROCESSOR card
r +5 Vdc from I/O card (via the Retic
Interface card)
r Retic Interface card
r LS sensor
2.13-8 PN 4235961E
CONTENTS
3
3 INSTALLATION PROCEDURES, 3.1-1
3.1 PREINSTALLATION CHECK, 3.1-1

Customer Training, 3.1-1
Carton Arrival, 3.1-1
Space and Accessibility, 3.1-1
Electrical Input, 3.1-1
Ambient Temperature and Humidity, 3.1-2
Ventilation, 3.1-2
Drainage, 3.1-3
Supplies, 3.1-3
3.2 INITIAL SETUP, 3.2-1
3.3 CONNECTING ASSEMBLIES, 3.3-1

Electronic Cable Connections, 3.3-1
Tubing Connections, 3.3-4
3.4 TESTING/CONFIGURING ASSEMBLIES, 3.4-1

Data Management System (DMS), 3.4-1
Software Installation, 3.4-1
Software Options Installation, 3.4-1
Bar-Code Reader Decoder Card Configuration, 3.4-1
Setting Up the Optional Printers, 3.4-1
3.5 INITIAL SYSTEM SETUP, 3.5-1

Setting Up the Institution Information, 3.5-1
Reagent System Setup, 3.5-1
Priming Reagents, 3.5-1
Adjusting the Hemoglobin Blank (Hgb Blank), 3.5-1
Adjusting the Blood/Bubble Detector Gains, 3.5-2
3.6 SYSTEM TESTING, 3.6-1

Performing System Test and Start Up, 3.6-1
Verifying General Operation of the Primary Mode on Instruments with a Rotary
Cap-Pierce Module, 3.6-2
Verifying General Operation of the Primary Mode on Instruments with an
Autoloader Module, 3.6-3
Verifying General Operation of the Secondary Mode, 3.6-5
3.7 ADJUSTMENTS AND CALIBRATION, 3.7-1

Entering Calibration Factors, 3.7-1
Measuring the RMS Noise, 3.7-1
Verifying the Diluent and Lytic Reagent Dispense Timing, 3.7-1
Verifying the CBC Latex Calibration, 3.7-1
Adjusting the Clog Detector Circuit, 3.7-1
Measuring the LS Current/LS Offset Voltage, 3.7-1
Verifying the DC Count and VCS Flow Rate, 3.7-2
PN 4235961E 3-i
CONTENTS
Verifying the Diff and Retic Latex Calibration, 3.7-2

Checking Reproducibility and Carryover in the Primary Mode, 3.7-2
Checking Reproducibility and Carryover in the Secondary Mode, 3.7-2
Checking Reproducibility and Carryover for the Retic Parameter, 3.7-3
Making Initial Primary-Mode Calibration Adjustments, 3.7-3
Verifying the Secondary Mode - to - Primary Mode Calibration, 3.7-3
Calibrating the Primary Mode with S-CAL® Calibrator, 3.7-4
Setting Up the Control Files and Running the Controls, 3.7-4
Completing the Installation Paperwork, 3.7-4
3.8 ACCOUNT/INSTRUMENT INFORMATION, 3.8-1

Purpose, 3.8-1
Account Information, 3.8-1
Installation Test Data Checklist, 3.8-1
(c = confirmed complete, * = Retic units only), 3.8-1
Installation Test Data Log Sheets, 3.8-2
A. System Test Data, 3.8-2
B. Startup Results, 3.8-3
C. RMS Noise Check Measurements (CBC), 3.8-4
D. RMS Noise Check Measurements (Diff and Retic), 3.8-4
E. CBC-Mode Latex Calibration, 3.8-5
F. Clog Detector Setup Results, 3.8-6
G. Light Current/LS Offset Measurements, 3.8-6
H. Five Patient Scatterplots Displaying DC Counts and Times, 3.8-7
I. Diff-Mode Latex Calibration (Five Consecutive Runs), 3.8-8
J. Retic-Mode Latex Calibration (Five Consecutive Runs), 3.8-9
K. Primary-Mode Reproducibility Run, 3.8-10
L. Primary-Mode Carryover Run, 3.8-11
M. Secondary-Mode Reproducibility Run, 3.8-12
N. Secondary-Mode Carryover Run, 3.8-13
O. Retic Mode-to-Mode Carryover Run, 3.8-14
P. Retic Within-Mode Carryover Run, 3.8-15
Q. Primary-Mode Initial Adjustment to 5C Normal Cell Control Cal
Factors, 3.8-16
R. Mode-to-Mode Calibration and Verification Runs/Secondary Mode
Calibration Factors, 3.8-17
S. S-CAL Calibrator Calibration Batch Tables/Cal Factors, 3.8-18
T. Control Results, 3.8-19
3.9 Setting Up The Optional Printers, 3.9-1

Graphic Printer Installation, 3.9-1
Anadex Ticket Printer Installation, 3.9-1
3.10 CONFIGURING THE MAXM ANALYZER FOR CYANIDE-FREE

REAGENTS, 3.10-1
Purpose, 3.10-1
3-ii PN 4235961E
CONTENTS
3
Hardware Changes, 3.10-1
Procedure, 3.10-1
Verification of the Hardware Changes, 3.10-3
Reagent Changes, 3.10-3
Pre-Conversion Instrument Verification, 3.10-3
Reagent Conversion, 3.10-4
Post-Conversion Instrument Verification, 3.10-4
ILLUSTRATIONS
3.2-1 MAXM Analyzer with Autoloader Module Main Component Layout, 3.2-1
3.2-2 TTM Baseplate Shipping Screws, 3.2-2
3.2-3 Compressor Shipping Pins, 3.2-3
3.2-4 Rocker Bed Lock-Down Screw, 3.2-5
3.3-1 Electronic Cable Connections, 3.3-1

3.3-2 Bar-Code Wand Connections, 3.3-2
3.3-3 DMS Line Voltage Select Switch, 3.3-3
3.3-4 Reagent and Drain Connections, 3.3-4
3.10-1 Reconfiguring the Instrument for the Cyanide-Free Reagent System, 3.10-2
TABLES
3.1-1 Fan and Vent Locations, 3.1-2
3.2-1 Deactivator Clip Locations, 3.2-2
PN 4235961E 3-iii
CONTENTS
3-iv PN 4235961E
3INSTALLATION PROCEDURES 3
3.1 PREINSTALLATION CHECK

ATTENTION: Japanese DMS configurations differ from normal configurations. Refer to the DMS
Configuration Listing for STKS™, MAXM™, and HmX Series Systems.
Ensure the following conditions are met before installing the instrument.
Customer Training
Verify that at least one person from the customer laboratory is scheduled to be trained.
Carton Arrival
1. Verify that all cartons arrived (based on customer order).
2. Verify that the cartons are undamaged. If damaged, confirm whether a claim was filed
with the carrier.
Space and Accessibility

Determine adequate space and accessibility as follows:
r Verify an island or movable table is available at the installation site to ensure easy access
for maintenance and servicing.
r Ensure the installation table can adequately support the MAXM analyzer which weighs
about 121 kg (266 lb).
r Verify the table is a comfortable work height and has sufficient space for the
individual units. Refer to Figure 3.2-1 for a typical layout.
t The Main Unit is 63.75 cm (25.10 in.) wide x 64.0 cm (25.20 in.) deep.
t The dimensions of the DMS, Ticket Printer, and Graphic Printer may vary,
depending on the models currently used.
r Verify a minimum clearance of 30 cm (12 in.) for access to the rear doors, plus sufficient
room for work space.
r Verify a minimum clearance of 30 cm (12 in.) on all sides.
Electrical Input
CAUTION Either of these two hazards can occur if you use an extension cord:
r Introduction of electrical interference can occur and cause instrument performance problems (frequent
lock ups and resets).
r Overheating, melting, and burning of the extension cord can occur.
Plug the primary power cable directly into the electrical outlet. Position the system close enough to an
electrical outlet so you do not need to use an extension cord.
r Verify that the female ac outlet that supplies power to the MAXM analyzer Electronic
Power Supply is located within 1.8 m (6 ft) of the space designated for the instrument.
r Verify with the house maintenance person that the socket is a three-wire outlet supplying
one of the following:
PN 4235961E 3.1-1
INSTALLATION PROCEDURES
PREINSTALLATION CHECK
t 100, 115, or 120 Vac (nominal voltages ±10%) at 20 A, 50/60 Hz, single-phase input
power
t 220, 230, or 240 Vac (nominal voltages ±10%) at 10 A, 50/60 Hz, single-phase input
power
r Verify with the house maintenance person that the ground path is capable of carrying the
full current of the circuit (that is, a confirmed third-wire earth ground).
r Verify that the circuit is independent and protected. To check for a dedicated line, do one
of the following:
t Ask the house maintenance person.
ATTENTION: Be sure to ask the customer’s permission before turning off any circuit
breakers.
t Plug a unit into the circuit and turn off the circuit breaker at the panel to see if
anything else is turned off. If anything else is turned off, the line is not dedicated.
r Verify that the neutral-to-ground potential does not exceed 0.5 Vac.
Ambient Temperature and Humidity

r Verify that the typical ambient temperature at the designated installation site is between
16° and 32°C (60° and 90°F).
r Verify that the humidity of the room is consistently no higher than 95% without
condensation.
r If the environment is air conditioned, verify that an additional 5,000 Btu is available to
compensate for the heat generated by the system.
Ventilation
Verify that all ventilation intake fans and exhaust fans would be at least 12 cm (5 in.) away
from any walls or obstructions that could interfere with the flow of air. The fans are located as
indicated in Table 3.1-1
Table 3.1-1 Fan and Vent Locations
Unit Fan and Vent Locations

Main Unit 1 exhaust fan on rear Analyzer module door and 1 intake fan at the
Pneumatic Power Supply. Intake vents all at rear of Main Unit.
Electronic Power Supply 1 exhaust fan on rear of the Electronic Power Supply.
DMS 1 exhaust fan on rear of the computer base.
Monitor (DMS) Models vary. At installation, check for location of fan and vents.
Graphic Printer (optional) Models vary. At installation, check for location of fan and vents.
3.1-2 PN 4235961E
PREINSTALLATION CHECK 3
Drainage
r If the waste from the instrument will drain into an open drain instead of a waste
container, verify that the drain is chemically resistant and is appropriate for
biohazardous waste.
r Verify that the drain or the waste container will be within 3.7 m (12 ft) of the Main Unit.
r Verify that the drain or the waste container is located so that the drain tubing will run in
a continuous downward slope to the drain, and the drain will be below the level of the
waste fittings.
Supplies
r Ensure the necessary paper supplies, printer paper, bar-code labels, and 10 mm to
16 mm (2 mL to "fat" 7 mL) evacuated blood collection tubes are available.
r Ensure the recommended reagents, calibrators and controls are present and within
expiration limits. Refer to Chapter 4 of the customer’s Reference manual for a list of
recommended reagents, controls, and calibrator.
PN 4235961E 3.1-3
PREINSTALLATION CHECK
3.1-4 PN 4235961E
INITIAL SETUP 3
3.2 INITIAL SETUP
1. Inspect all boxes for damage. Notify shipping of any external damage.
2. Uncrate the Electronic Power Supply module and the Main Unit. Place the Main Unit in
its proper location (Figure 3.2-1).
Note: You will install the Electronic Power Supply module in a later step.
3. Ensure the Electronic Power Supply and Main Unit are intact, with no broken, cracked,
or visually defective parts.
4. Uncrate the DMS, the Ticket Printer (optional) and the Graphic Printer (optional). Place
them in their proper locations (Figure 3.2-1).
IMPORTANT Positioning the DMS too close to the right side of the Main Unit can cause electronic
interference, which can affect platelet and white blood cell counts. If the customer prefers to have the DMS
on the right side of the Main Unit, position the DMS monitor at least 30 cm (1 ft) from the Main Unit.
Figure 3.2-1 MAXM Analyzer with Autoloader Module Main Component Layout
Main Unit
DMS
RBC
WBC
V REL#
O
L
U
M
E fL
50 100 200 300
PLT
REL#
DF 1 fL
ID#1: WBC 5.5 2 10 20
RBC
30
5.29
ID#2: % H
HGB 10.4
NE 53.6 3.8 HCT 44.0
Data: 01/09/89 LY 38L6 2/1 MCV 83.1
Time: 10:10:09 MD 4.6 0.9 MCH 31.9 H
ED 2.0 0.1 MCHC 41.9 H
BA 1.2 0.1 RDW 15.2 H
PLT 21.4
MPV 9.0
®
F3- Run F4-Print F5- Other F6-DF F7-AP F8-DB F9-Menu

F1 Help F2-Ticket
Graphic Printer
5961224D
5. Ensure that the DMS and the Printers are intact, with no broken, cracked, or visually
defective parts.
6. Open the front, right-side, and right rear doors, covers, and panels of the Main Unit to
access the pinch valves and the TTM.
7. Check the tubing in the pinch valves and remove the deactivator clips:
a. Remove the Main Diluter module support bracket and loosen the two 5/16-in. hex
nuts that secure the top of the Main Diluter module.
b. Inspect all the I-beam tubing using a dental mirror to make sure that the tubing is
properly installed.
Note: Unfastening the captive screws for the CBC module and placing the module
in its mounting hinges makes it easier to check this tubing.
PN 4235961E 3.2-1
INITIAL SETUP
c. Remove all pinch valve deactivator clips (49 single-action valves, 9 triple-action
valves).
Note: The pinch valve deactivator clips listed in Table 3.2-1 are not conspicuous.
Ensure that they are removed.
Table 3.2-1 Deactivator Clip Locations
Pinch Valve Located on the...

PV20 Pump module, behind the decorative snap-on panel. See Figure A.5-10, Pump Module, Old
PV70 Configuration, or Figure A.5-11, Pump Module, New Configuration.
PV8 Rear of the CBC module. See Figure A.5-5, CBC Module, Rear View.
PV66
PV67
PV49 Top of the backwash pump bracket. See Figure A.5-7, Main Diluter Module, Right-Side
View.
PV61 Bracket on the inside of the upper rear Diluter door. See Figure A.5-7, Main Diluter Module,
PV62 Right-Side View.
PV63
PV64
PV65
d. Reinstall the Main Diluter module support bracket, tighten the two 5/16-in. hex
nuts that secure the top of the Main Diluter module, and reinstall the CBC module if
it is out.
8. Remove the TTM shipping materials:
a. Remove the plastic TTM splash shield, the flow-cell cover, and the laser cover to
access the shipping materials.
b. Remove the three shipping screws from the TTM baseplate. See Figure 3.2-2.
Figure 3.2-2 TTM Baseplate Shipping Screws

SIDE VIEW REAR VIEW
COVERS
LASER RADIATION WHEN

OPEN AND INTERLOCK
DEFEATED
AVOID EYE OR SKIN
EXPOSURE TO DIRECT OR
SCATTERED RADIATION
2413943
SHIPPING SHIPPING SHIPPING

5961030C
PAD SCREWS SCREW
3.2-2 PN 4235961E
INITIAL SETUP 3
c. Lift up on the TTM baseplate, and pull out the styrofoam shipping pad.
d. Reinstall the flow-cell cover, laser cover, and the TTM splash shield.
9. Close the front, right-side, and right rear doors, covers, and panels of the Main Unit.
10. Remove the shipping pins from the Pneumatic Power Supply, and configure the
Pneumatic Power Supply for the correct ac input voltage:
a. Remove the screws that secure the Pneumatic Power Supply to the rear of the
Main Unit.
b. Pull out the Pneumatic Power Supply.
c. Remove the two shipping pins from the compressor support posts (Figure 3.2-3).
Figure 3.2-3 Compressor Shipping Pins
Compressor
support posts
(8)
Shipping pins
7535041A
d. Measure the ac line voltage at the socket into which the unit will be plugged, and
record the voltage on the Instrument Reference Form.
e. Configure the Pneumatic Power Supply Buck-Boost Transformer Terminal card to
the appropriate configuration for the ac input line voltage measured in step d. Refer
to Table A.3-6, Pneumatic Power Supply Buck-Boost Transformer Terminal Card
Jumpers and Connections.
f. If you changed the configuration of the Pneumatic Power Supply Buck-Boost
Transformer Terminal card, replace the UNIT CONFIGURATION label on the left
side of the Pneumatic Power Supply with one of the new UNIT CONFIGURATION
labels included in the installation kit and mark the new label appropriately.
g. Slide the Pneumatic Power Supply back into place, and reinstall the screws to secure
the Pneumatic Power Supply in position.
PN 4235961E 3.2-3
INITIAL SETUP
11. Unpack the reagent wash bottles (in accessory kit) and leave them with the customer.
12. Remove the shipping materials from the BSV:
a. Loosen the BSV knob.
b. Remove all the spacers and the instruction tag from the BSV.
c. Rinse between the faces of the BSV with distilled water.
d. Tighten the BSV knob.
13. Inspect, configure, and install the Electronic Power Supply.
a. Remove the Electronic Power Supply cover.
b. Verify that all Electronic Power Supply Terminal card (TB) jumpers and
connections are configured for the ac input line voltage measured in step 10 d. Refer
to Table A.3-3, Electronic Power Supply Terminal Card Jumpers and Connections.
c. If you changed the configuration of the Electronic Power Supply Terminal card,
replace the UNIT CONFIGURATION label on the rear of the Electronic Power
Supply and mark the new label appropriately.
d. Reinstall the Electronic Power Supply cover.
e. Open the rear Analyzer module doors.
CAUTION The opening for the Electronic Power Supply contains wiring harness connectors which could be
jammed behind the Electronic Power Supply. As you slide in the Electronic Power Supply, access the wiring
harness connectors from the rear of the Main Unit and move them as needed to avoid jamming.
f. At the front of the Main Unit, position the Electronic Power Supply on the guide
rails in its designated opening. Carefully push and slide the Electronic Power
Supply into the opening.
g. Connect the ground wire (green with yellow stripe) to E8 on the top rear edge of the
Electronic Power Supply chassis.
Note: Push the Electronic Power Supply slightly forward to expose the ground
E-Point.
h. Connect wiring harness plugs P105, P107, P108, P112, P113, P114, P115, and P116
to their respective connectors on the Electronic Power Supply. (P119 is not
connected.)
i. Ensure all the connectors on the rear of the Analyzer module are seated correctly.
j. Secure the Electronic Power Supply retaining bracket at the front left of the
Electronic Power Supply.
14. Configure the Laser Power Supply for the correct ac input voltage:
a. Remove the cover of the Laser Power Supply, mounted on the inside of the right rear
Analyzer module door.
b. Verify that the Laser Power Supply buck-boost transformer jumpers and wiring
connections are configured for the ac input line voltage measured in step 10 d. Refer
to Table A.3-5, Laser Power Supply Buck-Boost Transformer Jumpers and
Connections.
3.2-4 PN 4235961E
INITIAL SETUP 3
c. If you changed the configuration of the Laser Power Supply buck-boost transformer,
replace the UNIT CONFIGURATION label under the Laser Power Supply and mark
the new label appropriately.
d. Reinstall the Laser Power Supply cover.
15. Close all the rear doors of the Main Unit.
16. On instruments with an Autoloader module, remove the shipping screw from the
Autoloader module:
a. Ensure that the upper and lower front doors of the Main Unit and the front
decorative snap-on panels are opened or removed.
b. Using the safety clip, remove the piercing needle cartridge and rest the cartridge on
the Pump module.
c. Locate the Autoloader module retaining brackets and loosen the two corresponding
Phillips-head screws that secure the brackets and module to the chassis. See
Figure 3.2-4.
Figure 3.2-4 Rocker Bed Lock-Down Screw
Retaining
brackets
M A N U A LS .
E R LY .R E FE R T O P R O D U C T
H A N D LE O R S TO R E N E E D LE
Lock-down
screw 7535042B
d. Gently pull the Autoloader module forward enough to expose the rocker bed
lock-down screw located on the right side of the Autoloader module. See
Figure 3.2-4.
e. Remove the Phillips-head lock-down screw to release the rocker bed.
f. Reinstall the Autoloader module, the needle cartridge, and the decorative panels.
17. Close the lower and upper front doors of the Main Unit.
18. Remove the shipping materials from the Graphic Printer. Refer to the Graphic Printer
User’s Manual.
19. Use the Installation Report Form to report anything that has an adverse effect on the
installation or instrument performance.
PN 4235961E 3.2-5
INITIAL SETUP
3.2-6 PN 4235961E
CONNECTING ASSEMBLIES 3
3.3 CONNECTING ASSEMBLIES
Electronic Cable Connections
IMPORTANT Improperly fastened interconnecting cable mounting hardware, such as screws or spring
clips, can induce electronic interference into the system. Ensure that all interconnecting cable mounting
hardware is properly fastened at the Main Unit and at the DMS.
1. Verify that all assemblies are turned off and disconnected from any power source.
2. Inspect all the cables, and ensure that the pins are straight and seated properly.
3. Connect the electronic cables as shown in Figure 3.3-1. If installing a second Graphic
Printer, see Parallel Card (LPT2) Installation in the DMS Configuration Listing for
STKS™, MAXM™, and HmX Series Systems.
Note: The exact location and type of connection on the back of the device may vary
depending on the specific device.
a. Connect one end of the video signals cable to the monitor and the other end to the
rear of the DMS computer.
b. Connect the monitor power cable to the monitor connector on the DMS computer
and to the power connector on the video display monitor.
Figure 3.3-1 Electronic Cable Connections

J1
COMPUTER AC Monitor power cable
Video signal cable
DMS Digital Communication box

P4
To bar-code wand
FJFNDKLDLNC
P1 P3
To host computer
Fsjcnlonl'pclsd, mcddcm
xxjk bb,Nhi hKJbkjjk kj
vhbjhjkbkbnlnnlkl
tyiu()*jknLONLK
P2
Graphic Ticket
Printer Printer
LPT1 Digiboard
To
keyboard Graphic
Printer
Power
cord signal
cable
5961001D
PN 4235961E 3.3-1
CONNECTING ASSEMBLIES
c. Connect the Graphic Printer signal cable to the parallel port (LPT1) on the DMS
computer and to the connector on the Graphic Printer.
d. Connect the main keyboard cable to the keyboard input connector on the rear of the
DMS computer. The auxiliary keyboard adapter cable may be required.
4. Locate the Digital Communication box and connect its cable to the Digiboard jack on
the DMS.
5. Connect three of the four outputs on the Digital Communication box as follows:
a. P1 to J1 on the right rear Analyzer door of the Main Unit
b. P2 to the single-ticket Printer (if applicable)
c. P3 to the host computer (if applicable).
6. Install the bar-code wand assembly:
a. Obtain the bar-code wand system components from the accessories kit:
r Bar-code wand assembly
r Bar-code wand holder
r P4 cable
b. Connect the P4 cable to the Digital Communication box.
c. Connect the D-25 type connector of the wand to the P4 cable on the Digital
Communication box. See Figure 3.3-2.
Figure 3.3-2 Bar-Code Wand Connections
Bar-code 2
wand 4
To DMS To Digital
To
keyboard Communication
keyboard
port PS-2 to XT box, P4
adapter
Wire ties
Beeper
(may be built into the wand
in newer versions) 5961273D
d. Connect the XT type connectors of the bar-code wand to the computer and to the
keyboard or the keyboard adapter if one is required. This connection provides
power to the wand.
3.3-2 PN 4235961E
e. Secure the connector and plug with wire ties as shown in Figure 3.3-2.
f. Obtain the double-sided adhesive strip provided with the wand stand, peel off the
paper on both sides to expose the adhesive, and affix the wand stand (larger end up)
to the side of the DMS at a location desired by the customer.
CAUTION Setting the line voltage select switch incorrectly can cause severe damage to the DMS. Be careful
to set the line voltage select switch correctly.
7. Verify that the DMS line voltage select switch (Figure 3.3-3) is set correctly to match the
voltage source being used, 115 Vac or 230 Vac.
Figure 3.3-3 DMS Line Voltage Select Switch
SWITCHED
115 PERIPHERAL
POWER OUTLET AC LINE INPUT
230
5961002C
8. Connect the DMS power cable to the ac line input connector on the DMS. Connect the
other end to the Main Unit’s Electronic Power Supply (COMPUTER AC) socket on the
rear of the Electronic Power Supply.
9. Connect the Graphic Printer/Plotter power cable from the Graphic Printer/Plotter to the
designated ac wall outlet (Figure 3.3-1).
10. If installing a single-ticket Printer, connect a power cable between the Printer and an ac
wall outlet (Figure 3.3-1).
11. Enable the on-board battery on the 376 CPU card:
a. Remove the shield from the Analyzer module.
b. Remove the 376 CPU card from the Analyzer module’s card cage.
c. Install jumpers X2, X3, and X4 on the 376 CPU card.
d. Reinsert the 376 CPU card into the card cage and secure it properly.
e. Reinstall the shield on the Analyzer module.
12. Ensure the POWER switch on the rear of the Electronic Power Supply is in the
OFF position.
13. Connect the ac input cable from the Electronic Power Supply to the designated ac socket
at the wall outlet.
PN 4235961E 3.3-3
Tubing Connections
IMPORTANT Reagent lines can introduce electronic interference into the system. To prevent electronic
interference from the reagent lines:
r Use the provided shielded lines for diluent, cleaning agent, CBC lytic reagent, PAK LYSE, PAK
PRESERVE, and waste.
r Separate the lines carrying reagents from electrical wires, ensuring the reagent lines do not contact
any power cables.
CAUTION Incomplete drainage and overflow into the vacuum system can occur if the waste line is too long,
or if the drain or waste container is elevated too high. For correct drainage, ensure:
r The waste line is 3.7 m (12 ft) or less.
r The drain or waste container is below the level of the waste fittings on the Main Unit.
1. Connect the reagent input lines and associated ground wires to the Main Unit as shown
in Figure 3.3-4.
Note: If connecting the waste line to an open drain instead of a waste container, install a
bypass jumper on the waste level sensor.
Figure 3.3-4 Reagent and Drain Connections
Ground J32
wires
LYSE
Waste level
sense
PAK Ground
LYSE wires
PAK
PRESERVE FJFNDKLDLNC
Fsjcnlo nl'pclsd, m cddcm
xxjkbb,N hi hKJbk j kkj
vhbjhjkb kbnlnn lkl
WASTE
tyiu()*jknLO NLK
DILUENT CLEANING
AGENT
Lytic PAK PAK

reagent LYSE PRESERVE
J34
P34
Diluent Waste Cleaning

agent
5961067D
3.3-4 PN 4235961E
2. Locate the labels for the reagent input lines and apply them to the unattached ends of
the lines.
Note: If you have sufficient labels, label both ends of the reagent input lines.
IMPORTANT Incorrectly installed reagents can affect reagent flow, and consequently instrument results. To
ensure proper reagent pickup and delivery, ensure that:
r Each tubing fits on its pickup tube at least 1/4 in. and that it is secured tightly with no leaks or kinks.
r The COULTER SCATTER PAK® (or MAXM PAK) and LYSE S® III diff lytic reagent containers are at
tabletop level.
r The waste container is clearly labeled WASTE and that the waste pickup tube is correctly installed.
3. Install the corresponding pickup tubes for the following reagents:

r Cleaning agent
r Diluent
r CBC Lytic reagent
r Cleaning agent
r PAK LYSE
r PAK PRESERVE
r Waste (if collecting the waste in a waste container)
4. If using a 500 mL or 1 L container of CBC lytic reagent, place the container in its
base holder.
5. Place a clear plastic reagent container neck support bracket on each of the following
containers: the diluent, the cleaning agent, and the waste (if collecting the waste in a
waste container).
.
IMPORTANT Allowing the waste line to enter the drain trap might introduce electronic noise into the
system, affecting the instrument results. When securing the waste tube in the drain, make sure the end of
the tube is not in the trap.
6. If you are connecting the waste line to an open drain, mechanically secure the waste tube
in the drain so the tube cannot accidentally come out of the drain. This prevents spillage.
7. If you installed a cyanide-free reagent system and if the BSV assembly on the instrument
uses air cylinders to rotate the BSV, reconfigure the Diluter hardware as directed under
Heading 3.10, CONFIGURING THE MAXM ANALYZER FOR CYANIDE-FREE
REAGENTS.
PN 4235961E 3.3-5
3.3-6 PN 4235961E
TESTING/CONFIGURING ASSEMBLIES 3
3.4 TESTING/CONFIGURING ASSEMBLIES
1. Turn ON the POWER switch on the rear of the Electronic Power Supply.
2. Turn ON (I) the Standby/Reset switch.
3. Verify that the fans on the rear of the Electronic Power Supply and on the rear door of the
Main Unit are working.
Data Management System (DMS)

1. Turn ON the computer. The computer performs a self-test which takes approximately
15 seconds.
2. If the DMS does not arrive at the C: prompt after it is turned ON, refer to the User’s
Guide or to the DMS Configuration Listing for STKS™, MAXM™, and HmX Series
Systems for the correct setup information.
Software Installation
Refer to the DMS Configuration Listing for STKS™, MAXM™, and HmX Series Systems for
software installation.
Software Options Installation

Refer to the DMS Configuration Listing for STKS™, MAXM™, and HmX Series Systems for
software installation.
Bar-Code Reader Decoder Card Configuration

Refer to Heading 4.38, BAR-CODE READER DECODER CARD CONFIGURATION.
Setting Up the Optional Printers

Refer to Heading 3.9, Setting Up The Optional Printers, for Printer installation.
PN 4235961E 3.4-1
TESTING/CONFIGURING ASSEMBLIES
3.4-2 PN 4235961E
INITIAL SYSTEM SETUP 3
3.5 INITIAL SYSTEM SETUP
Setting Up the Institution Information
1. From the Access screen, select Main Menu tt Special Functions tt Set Up tt System Set Up tt
Institution.
2. Enter the appropriate information requested on the screen.
3. Press Ò to save data and escape.
Reagent System Setup

1. From the System Set Up screen, select Reagents.
2. Use the numeric keys to enter the correct reagent lot numbers and appropriate
expiration dates based on open vial stability requirements for each
recommended reagent:
r ISOTON III® diluent = expiration date on container
r LYSE S III diff lytic reagent = 60 days for 1 liter and 5 liter
r LYSE S III diff lytic reagent = 30 days for 500 mL
r COULTER CLENZ® cleaning agent = 90 days
r COULTER SCATTER PAK (or MAXM PAK), PAK LYSE and PAK PRESERVE
reagents = 60 days.
3. Press Ò to save data and escape.
4. Press ÈÈ to return to the Special Functions menu.
5. From the Special Functions menu, select Diagnostics tt Operator Options tt Fluidic Test tt
Disable Reagent Sensors.
6. Ensure all reagent sensors are ON.
Note: Select Waste = OFF if unit is draining directly into a plumbing system.
7. Press Û to save data and escape.
8. Press Ñ to return to the Main menu.
Priming Reagents
ATTENTION: More than one priming routine may be required to completely prime a new
system.
1. From the Main menu, select Diluter Functions tt Prime Reagents tt All to prime all reagents
(<10 minutes).
2. As reagents are priming, verify that each reagent input line fills and that no leaks occur
within the Diluter system.
3. If air appears in the respective reagent systems, repeat steps 1 and 2.
Adjusting the Hemoglobin Blank (Hgb Blank)

1. Do the Hemoglobin Lamp Adjustment procedure under Heading 4.27, HEMOGLOBIN
PREAMP MODULE REPLACEMENT.
PN 4235961E 3.5-1
INITIAL SYSTEM SETUP
2. Print out (online manual) or copy (printed manual) Heading 3.8,

ACCOUNT/INSTRUMENT INFORMATION. This section includes the Installation Test
Data Checklist and the Installation Test Data Log Sheets.
3. On the Installation Test Data Checklist, check off Hgb Lamp Adjustment.
Note: As you complete each test in the installation procedure, check off the procedure on
the Installation Test Data Checklist and, when appropriate, attach printouts or record the
data on the Installation Test Data Log Sheets.
Adjusting the Blood/Bubble Detector Gains

1. Adjust the blood/bubble detector gains as directed under Heading 4.21, BLOOD/BUBBLE
DETECTORS GAIN ADJUSTMENT.
2. On the Installation Test Data Checklist, check off Bubble/Blood Detectors Gain
Adjustment.
3.5-2 PN 4235961E
SYSTEM TESTING 3
3.6 SYSTEM TESTING
Tools/Supplies Needed
B One bar-code labeled specimen tube with clean diluent
B On instruments with a Rotary Cap-Pierce module, one bar-code labeled specimen tube of
whole blood
B On instruments with an Autoloader module, ten bar-code labeled specimen tubes of
whole blood
Performing System Test and Start Up

1. Bypass the lower front door switch with a magnet.
2. Perform a System Test and log the results.
a. From the Main menu, select Special Functions tt Diagnostics tt Operator Options tt
System Test to access the System Test Run screen.
b. Press Ë RUN to initiate the System Test and adjust appropriate pressure and/or
vacuum regulators as required to ensure correct levels.
c. Rerun the System Test and verify that all electronic and pneumatic readouts pass.
d. Print out the System Test data and attach the printout (or record the data) as item A
on the Installation Data Log Sheets.
e. On the Installation Test Data Checklist, check off System Test Data.
f. Press Ñ to return to the Main menu.
ATTENTION: If the upper front door is open, the background results may be out of limits.
3. Perform a Startup cycle and print out the results.

a. Ensure the upper front door is closed.
b. From the Main menu, select Diluter Functions.
c. Press S to select Start Up, and then press Û to initiate the cycle.
ATTENTION: Under certain fail conditions the instrument does not complete the Startup
cycle; it stops and displays an error message.
d. When the Startup cycle is completed, verify all startup results Pass.
Note: At the end of the Startup cycle, the instrument automatically prints the
startup results. You can check the results on the printouts or on the screen. Press
Ê to access the Detailed Startup Results screen from the Pass/Fail screen.
r If all startup results Pass, log the results; go to step 5.
r If startup results do not Pass, correct the problem; go to step 4.
4. If the startup results did not pass:
a. Review the startup results to determine the cause of the Fail status.
b. Locate and correct the problem.
PN 4235961E 3.6-1
SYSTEM TESTING
c. Perform a System Test:

1) Select Special Functions tt Diagnostics tt Operator Options tt System Test to access
the System Test Run screen.
2) Press Ë RUN to initiate the System Test.
d. Repeat from step a until the all the system tests Pass.
e. Repeat step 3 above to recheck the Startup cycle.
5. Attach the startup test results printout (or record the data) as item B on the Installation
Data Log Sheets.
6. On the Installation Test Data Checklist, check off Startup Test Data.
Verifying General Operation of the Primary Mode on Instruments with a Rotary Cap-Pierce
Module
1. From the Main menu (press Ñ to return to the Main menu if necessary), select Sample
Analysis tt Run Samples.
2. Ensure DIFF is ON.
3. Press Ê Start Primary. The instrument processes the request and displays S/A 1 °MODE
ON in the lower right corner (System Status field) of the DMS.
4. Cycle a sample of clean diluent and verify the system is free of liquid and air leaks:
a. Disable the blood/bubble detector (Main tt Special Functions tt Diagnostics tt Operator
Options tt BSV Tests tt Blood Detector).
b. Hold the tube’s label in front of the reader.
c. As soon as the instrument displays the green light and beeps, place the tube in the
entry port of the carousel assembly to initiate the cycle.
d. Ensure the system is not leaking, paying particular attention to the aspiration path.
ATTENTION: You may need to perform steps 5 and 6 more than once to complete
all observations.
5. Cycle a sample of whole blood:
a. Mix the specimen tube.
b. Hold the tube’s label in front of the reader.
c. As soon as the instrument displays the green light and beeps, place the tube in the
entry port of the carousel assembly to initiate the cycle.
ATTENTION: Step 6 assumes a knowledge of correct instrument operation.
6. Observe the cycles and verify the following:

r At the bar-code reader and carousel assembly -
t Bar-code reader turns on when a tube is placed in front of the scanner.
t Green and red LEDs change state.
t Carousel assembly advances the tube to the correct piercing position.
t Carousel assembly unloads the specimen tube into the exit tray.
3.6-2 PN 4235961E
SYSTEM TESTING 3
t Green and red LEDs change state.
t No Rotary Cap-Pierce errors occur.
r At the Piercing Station and the BSV and CBC modules -
t Needle pierces and retracts correctly.
t Needle bellows drain completely each time.
t Needle vent line is rinsed and air-dried properly.
t Whole blood is aspirated correctly with no aspiration errors.
t BSV center section rotates smoothly and completely.
t Aperture baths and waste chamber drain properly.
t Aperture baths fill to appropriate sample levels accompanied by mixing
bubbles.
t Lytic reagent is delivered to the WBC bath.
t Count pinch valves open completely providing proper aperture flow rate.
t Aspiration lines are completely backwashed.
t Cross rinse to the baths occurs in correct proportions.
r At the Mixing Chamber module -
t Mixing chamber drains and rinses completely.
t Mixing chamber mixes at the proper rate and times.
t Erythrolyse II reagent and sample delivery appears sufficient.
t StabiLyse reagent delivery appears sufficient.
r At all modules -
t No liquid leaks occur within the system.
t No air leaks occur within the system.
7. If the above checks pass, continue to the section, Verifying General Operation of the
Secondary Mode. If not, troubleshoot and correct the problems before continuing.
Verifying General Operation of the Primary Mode on Instruments with an Autoloader

Module
a. Disable the blood/bubble detector (Main tt Special Functions tt Diagnostics tt Operator
b. Load a cassette with the tube of diluent, positioning the tube to expose the bar-code
label through the top opening in the cassette position.
c. Place the cassette in the loading bay to initiate the cycle.
d. Ensure the system is not leaking, paying particular attention to the aspiration path.
PN 4235961E 3.6-3
SYSTEM TESTING
5. Load two cassettes with five blood specimen tubes each, positioning the tubes to expose
the bar-code labels through the top opening in each cassette position.
ATTENTION: You may need to perform steps 6 and 7 more than once to complete
all observations.
6. Place the cassettes in the loading bay to initiate the cycle.
ATTENTION: Step 7 assumes a knowledge of correct instrument operation.
7. Observe the cycles and verify the following:

r At the rocker bed and cassette mechanism -
t Right elevator moves up and down, properly placing each cassette onto rocker
bed.
t Rocker bed rocks smoothly and freely.
t Specimen tubes are properly positioned at the piercing station.
t Cassettes are transported across the rocker bed completely.
t Left elevator moves up and down, placing each cassette into unloading bay.
t Bar-code scanner turns ON and reads each label for ID#1 and Cass/Pos#.
t No Autoloader transport station errors occur.
r At the Piercing Station and the BSV and CBC modules -
t Needle pierces and retracts correctly.
t Needle bellows drain completely each time.
t Needle vent line is rinsed and air-dried properly.
t Whole blood is aspirated correctly with no aspiration errors.
bubbles.
t Aspiration lines are completely backwashed.
r At all modules -
3.6-4 PN 4235961E
SYSTEM TESTING 3
8. If the above checks pass, continue to the section, Verifying General Operation of the
Secondary Mode. If not, troubleshoot and correct the problems before continuing.
Verifying General Operation of the Secondary Mode

1. Press ËË to change to the Secondary mode. The instrument processes the request
and displays S/A 2 °MODE ON in the System Status field.
2. Type in a patient ID number at the DMS keyboard and then press Û to identify the
specimen before processing a sample.
Note: All specimens must be identified to cycle the instrument.
a. Uncap the specimen tube of diluent
b. Immerse the BSV aspirator tip in the tube.
c. Press the sample bar to begin the cycle.
d. Ensure the system is not leaking, paying particular attention to the aspiration
pathway.
ATTENTION: It may be necessary to repeat step 4 more than once to complete all observations.
4. Cycle a sample of whole blood:

a. Repeat step 2 to identify the specimen.
b. Mix and uncap the specimen tube.
c. Immerse the BSV aspirator tip in the tube.
d. Press the sample bar to begin the cycle.
5. Observe and verify the following during the Secondary-mode cycle:
r At the BSV module -
t Whole blood is properly aspirated into the BSV.
t Rinse block moves down and back up the aspirator tip smoothly, completely
washing the outside of the tip by the end of the cycle.
t Rinse block does not leak.
t Aspirator lines are completely backwashed by the end of the cycle.
t Aspirator tip does not drip anytime during the cycle.
r At the CBC module -
bubbles.
PN 4235961E 3.6-5
SYSTEM TESTING

r At all modules -
6. If the checks in step 5 pass, check the background count. Go to step 7. If the checks did
not pass, troubleshoot and correct the problems before continuing.
7. Check the background count:
a. Cycle clean diluent in the Secondary-mode.
b. Ensure the background results are within the limits specified in Table A.1-5,
Primary/Secondary-Mode Background Counts.
c. On the Installation Test Data Checklist, check off Secondary-Mode Background.
3.6-6 PN 4235961E
ADJUSTMENTS AND CALIBRATION 3
3.7 ADJUSTMENTS AND CALIBRATION
Entering Calibration Factors
1. From the Main menu, select Special Functions tt Calibration tt Enter Calibration Factors.
2. Enter the Primary-mode calibration factors provided in the documents shipped with the
instrument.
Measuring the RMS Noise

1. Measure the RMS noise for CBC. Refer to CBC RMS Noise Checks under Heading 4.12,
RMS NOISE CHECKS.
2. Record the results in the table under item D on the Installation Test Data Log Sheets.
3. Measure the RMS noise for Diff, and for Retic if applicable. Refer to Diff and Retic RMS
Noise Checks, under Heading 4.12, RMS NOISE CHECKS.
4. Record the results in the table under item E on the Installation Test Data Log Sheets.
5. On the Installation Test Data Checklist, check off RMS Noise Measurements.
Verifying the Diluent and Lytic Reagent Dispense Timing

1. Perform the procedure under Heading 4.20, LYTIC REAGENT AND DILUENT
DELIVERY TIMING CHECK.
2. On the Installation Test Data Checklist, check off Diluent/Lytic Reagent Dispense
Timing.
Verifying the CBC Latex Calibration

1. Perform the CBC Latex Calibration and Verification under Heading 4.4, LATEX
CALIBRATION AND VERIFICATION.
2. Print out the RBC and WBC counts, modes and aperture current, and attach the printout
as item J on the Installation Test Data Log Sheets.
3. On the Installation Test Data Checklist, check off CBC Latex Calibration.
Adjusting the Clog Detector Circuit

1. Adjust the clog detector circuit as directed in Clog Detector Circuit Adjustment under
Heading 4.11, RF DETECTOR PREAMP CARD ADJUSTMENTS.
2. Record the ambient temperature and TP2 voltage as item C on the Installation Test Data
Log sheets.
3. On the Installation Test Data Checklist, check off Clog Detector Setup Results.
Measuring the LS Current/LS Offset Voltage

1. Perform the Laser On Current/LS Offset Voltage Check procedure under Heading 4.23,
VOLTAGE CHECKS AND ADJUSTMENTS.
2. Record the results in the table under item F on the Installation Test Data Log Sheets.
3. On the Installation Test Data Checklist, check off Light Current/LS Offset Measurement.
PN 4235961E 3.7-1
ADJUSTMENTS AND CALIBRATION
Verifying the DC Count and VCS Flow Rate

1. Adjust the VCS flow rate as directed under Heading 4.10, VCS FLOW-RATE
ADJUSTMENT.
2. Print out five patient scatterplots with DC counts and times and attach the printouts as
item G on the Installation Test Data Log Sheets.
3. On the Installation Test Data Checklist, check off Flow-Rate/DC Count Verification.
Verifying the Diff and Retic Latex Calibration

1. Perform the Diff and Retic Latex Calibration and Verification procedure under
Heading 4.4, LATEX CALIBRATION AND VERIFICATION.
2. Attach the printouts from the five runs in the Diff-Latex Calibration mode as item H on
the Installation Data Test Log Sheets.
3. If the MAXM analyzer has the Retic option, attach the printouts from the five runs in the
Retic-Latex Calibration mode as item I on the Installation Test Data Log Sheets.
4. On the Installation Test Data Checklist, check off Diff Latex Calibration and Retic Latex
Calibration.
Checking Reproducibility and Carryover in the Primary Mode

1. Perform the Verifying Reproducibility in the Primary Mode procedure under
Heading 4.5, REPRODUCIBILITY CHECKS.
2. Print out the completed reproducibility table and attach it as item K on the Installation
Test Data Log Sheets.
3. Perform the Checking Carryover in the Primary Mode procedure under Heading 4.6,
CARRYOVER CHECKS.
4. Print out the Primary-mode carryover results and attach the printout as item L on the
Installation Test Data Log Sheets.
5. On the Installation Test Data Checklist, check off Primary-Mode Reproducibility and
Primary-Mode Carryover.
Checking Reproducibility and Carryover in the Secondary Mode

1. Perform the Verifying Reproducibility in the Secondary Mode procedure under
2. Print out the completed reproducibility table and attach it as item M on the Installation
3. Perform the Checking Carryover in the Secondary Mode procedure under Heading 4.6,
CARRYOVER CHECKS.
4. Print out the Secondary-mode carryover results and attach the printout as item N on the
5. On the Installation Test Data Checklist, check off Secondary-Mode Reproducibility and
Secondary-Mode Carryover.
3.7-2 PN 4235961E
ADJUSTMENTS AND CALIBRATION 3
Checking Reproducibility and Carryover for the Retic Parameter
1. Determine if the customer plans to use the Retic mode and if the reagents for preparing
blood samples for retic analysis on the instrument are available in the laboratory.
r If the customer will use the Retic mode and the reagents are available, go to step 2 to
check reproducibility in the Retic mode.
r It the customer will not use the Retic mode, or if the reagents are not available, go to
Making Initial Primary-Mode Calibration Adjustments below.
2. Perform the Verifying Retic % Reproducibility (Sample Analysis) procedure under
3. Perform the Retic Mode-to-Mode Carryover procedure under Heading 4.6, CARRYOVER
CHECKS, Retic Carryover Tests.
4. Attach the printouts of the Retic mode-to-mode carryover results as item O on the
5. Perform the Retic Within Mode procedure under Heading 4.6, CARRYOVER CHECKS,
Retic Carryover Tests.
6. Attach the printouts of the Retic within mode carryover results as item P on the
7. On the Installation Test Data Checklist, check off the Retic-Mode Reproducibility and
Retic-Mode Carryover.
Making Initial Primary-Mode Calibration Adjustments

1. Run 5C® normal cell control and verify the results fall within the expected ranges.
r If the results are within the expected ranges, go to Verifying the Secondary Mode -
to - Primary Mode Calibration below.
r If the results are not within the expected ranges, go to step 2 to perform the initial
adjustments to the Primary-mode calibration factors.
2. Perform the procedure under Heading 4.7, INITIAL ADJUSTMENT OF
PRIMARY-MODE CALIBRATION FACTORS.
3. Print out the calibration data and attach the printout as item Q on the Installation Test
Data Log Sheets.
4. On the Installation Test Data Checklist, check off Initial Calibration Adjustment/Cal
Factors.
Verifying the Secondary Mode - to - Primary Mode Calibration

1. Perform the procedure under Heading 4.8, SECONDARY MODE-TO-PRIMARY MODE
CALIBRATION.
2. Print out the calibration runs data and attach the printout as item R on the Installation
3. Print out the verification runs data and attach the printout as item R on the Installation
4. On the Installation Test Data Checklist, check off Mode-to-Mode Calibration/Cal
Factors.
PN 4235961E 3.7-3
ADJUSTMENTS AND CALIBRATION
Calibrating the Primary Mode with S-CAL® Calibrator

1. Assist the customer in performing the S-CAL calibrator calibration procedure as outlined
in the operator’s Special Procedures and Troubleshooting Manual.
2. Ensure that a copy of the calibration data is placed into a log book prepared for the
instrument and indicate the importance of maintaining an accurate calibration
factor record.
3. Print out the calibration batch tables and attach the printouts as item S on the
4. On the Installation Test Data Checklist, check off S-CAL Calibrator Batch Tables/Cal
Factors.
Setting Up the Control Files and Running the Controls

1. Help the customer set up the control files for 5C cell control, 4C® PLUS cell control,
Retic-C™ control, and LATRON™ control as outlined in the Software Menu section of
the Operator's Guide and in the Training Guide.
2. Help the customer run the controls, printout the results, and verify the results fall within
the expected ranges.
3. Attach the printouts of the control results as item T on the Installation Test Data Log
Sheets.
4. On the Installation Test Data Checklist, check off Control Results.
Completing the Installation Paperwork

1. Complete the Instrument Installation Report form.
2. Complete the Account/Instrument Information and Instrument Data Log Sheets and
insert them into the Installation section of the Instrument’s Certification Book.
3. Using the data obtained during installation, complete the COULTER® MAXM Analyzer
Instrument Reference Form.
3.7-4 PN 4235961E
ACCOUNT/INSTRUMENT INFORMATION 3
3.8 ACCOUNT/INSTRUMENT INFORMATION
Purpose
Complete all tables and attach required printouts as indicated within the following pages.
Obtain the customer’s signature upon completion, and place this document in the Installation
section of the Instrument’s Certification Book.
Account Information
Account Name:
Contact:
Address:
Telephone #:
Date of Installation:
Installer Name and Employee #:
Component Model and Serial #

Analyzer
Data Management System (DMS)
Ticket Printer
Graphic Printer
Installation Test Data Checklist

(c = confirmed complete, * = Retic units only)
Test Data (Item on the Log Sheets) Test Data (Item on the Log Sheets)
Hgb Lamp Adjustment Diff Latex Calibration - five runs (Item I)
Blood/Bubble Detector Gain Adjustment Retic Latex Calibration - five runs* (Item J)
System Test Data (Item A) Primary-Mode Reproducibility (Item K)
Startup Test Data (Item B) Primary-Mode Carryover (Item L)
Secondary-Mode Background Secondary-Mode Reproducibility (Item M)
Primary-Mode Calibration Factor Entry Secondary-Mode Carryover (Item N)
RMS Noise Measurements (Items C, D) Retic-Mode Reproducibility*
Lytic Reagent/Diluent Delivery Timing Retic-Mode Carryover* (Items 0, P)
Check
CBC Latex Calibration (Item E) Initial Calibration Adjustments/Cal Factors
(Item Q)
Clog Detector Setup Results (Item F) Mode-to-Mode Calibration/Cal Factors
(Items R)
Light Current/LS Offset Measurement S-CAL® Calibrator Batch Tables/Cal Factors
(Item G) (Item S)
Flow-Rate/DC Count Verification - five Control Results (Item T)
patient scatterplots (Item H)
PN 4235961E 3.8-1
ACCOUNT/INSTRUMENT INFORMATION
Installation Test Data Log Sheets

Attach printouts or log data in the areas below.
A. System Test Data
3.8-2 PN 4235961E
B. Startup Results
PN 4235961E 3.8-3
C. RMS Noise Check Measurements (CBC)
TP* Measurement
RBC
WBC
PLT
* Read with a true RMS meter while the instrument is in the idle state.
D. RMS Noise Check Measurements (Diff and Retic)
Diff Mode Measurements Retic Mode Measurements
RMS Noise TP Static Dynamic Static Dynamic

V
C
S
3.8-4 PN 4235961E
E. CBC-Mode Latex Calibration
PN 4235961E 3.8-5
F. Clog Detector Setup Results

Ambient temperature: °F or °C
TP2 = Vdc
G. Light Current/LS Offset Measurements
Test Point Reference Diff Mode Measurement Retic Mode Measurement

LS Preamp 5 TP1-TP3
LS Sensor Light current:
Dark current:
3.8-6 PN 4235961E
H. Five Patient Scatterplots Displaying DC Counts and Times
PN 4235961E 3.8-7
I. Diff-Mode Latex Calibration (Five Consecutive Runs)
3.8-8 PN 4235961E
J. Retic-Mode Latex Calibration (Five Consecutive Runs)
PN 4235961E 3.8-9
K. Primary-Mode Reproducibility Run
3.8-10 PN 4235961E
L. Primary-Mode Carryover Run
PN 4235961E 3.8-11
M. Secondary-Mode Reproducibility Run
3.8-12 PN 4235961E
N. Secondary-Mode Carryover Run
PN 4235961E 3.8-13
O. Retic Mode-to-Mode Carryover Run
3.8-14 PN 4235961E
P. Retic Within-Mode Carryover Run
PN 4235961E 3.8-15
Q. Primary-Mode Initial Adjustment to 5C Normal Cell Control Cal Factors
3.8-16 PN 4235961E
R. Mode-to-Mode Calibration and Verification Runs/Secondary Mode Calibration Factors
PN 4235961E 3.8-17
S. S-CAL Calibrator Calibration Batch Tables/Cal Factors
3.8-18 PN 4235961E
T. Control Results
Customer Signature:
Coulter Representative Signature:
PN 4235961E 3.8-19
3.8-20 PN 4235961E
Setting Up The Optional Printers 3
3.9 Setting Up The Optional Printers

Graphic Printer Installation
Refer to that printer’s user guide for instructions for the Graphic Printer.
Anadex Ticket Printer Installation

1. Remove the cover of the Printer and set the internal DIP switches on the Printer Logic
Control card to:
r Switch 1; position 1 ON, all others OFF
r Switch 2; positions 1, 3, 4, and 5 ON, all others OFF.
2. Verify operation of the Printer:
a. Turn ON the Printer.
b. Insert a ticket into the Printer and verify the ticket properly feeds into the Printer.
c. Turn OFF the Printer.
d. Locate the black self-test switch, labeled TEST SW, in the upper left corner of the
Printer Logic Control card.
e. Press and hold the self-test switch and turn ON the Printer. Do not release the
self-test switch until the ticket fully ejects.
Note: The top line displays Test Failed if pins 2 and 3 on the rear input connector
are not jumped with a paper clip; this is normal.
PN 4235961E 3.9-1
Setting Up The Optional Printers
3.9-2 PN 4235961E
CONFIGURING THE MAXM ANALYZER FOR CYANIDE-FREE REAGENTS 3
3.10 CONFIGURING THE MAXM ANALYZER FOR CYANIDE-FREE REAGENTS
Purpose
Use the procedures in this section to convert the MAXM analyzer to a cyanide-free reagent
such as:
r LYSE S® 4 lytic reagent

r ISOTON® 4 diluent
Note: LYSE S 4 lytic reagent, the CBC lytic reagent without cyanide, must be used in
combination with ISOTON 4 diluent only.
Currently two different BSV assemblies are in use in the field; one uses air cylinders to rotate
the BSV, the other uses an actuator.
.
IMPORTANT On instruments using a BSV assembly with air cylinders, if a new cyanide-free reagent system
is installed with the current hardware configuration microbubbles can interfere with the Hgb background
and reproducibility results. Before installing the cyanide-free reagent system, reconfigure the hardware to
reduce the production of microbubbles.
r On instruments that use a BSV assembly with air cylinders, use the Hardware Changes
procedure to reconfigure the hardware before installing the cyanide-free reagents.
Note: The hardware changes described in this procedure reduce the microbubbles in the
WBC bath by slowing down the movement of diluent from the RBC diluent dispenser
into the WBC bath.
IMPORTANT On instruments using a BSV assembly with an actuator, reconfiguring the Diluter hardware
before installing a cyanide-free reagent system could increase the Hgb background and the diff flagging.
When converting one of these instruments, DO NOT reconfigure the Diluter hardware.
r On instruments that use a BSV assembly with an actuator, go directly to the Reagent
Changes procedure.
Hardware Changes
B 30.5 cm (1 ft) tubing, polyurethane, 0.113 i.d., PN 3202038
B One Y-fitting, 0.085 i.d., PN 1018245 (replacement for FY8)
B One choke, pneumatic, black metal, 0.010 orifice, PN 6213011 (replacement for CK32)
B One union fitting, 0.125 to 0.062 i.d., PN 6232564
B 5C cell control, all levels
Procedure
1. Open the front door of the instrument and check the configuration of the BSV assembly.
r If the BSV assembly uses air cylinders, go to step 2.
r If the BSV assembly uses an actuator, skip to the Reagent Changes procedure.
PN 4235961E 3.10-1
CONFIGURING THE MAXM ANALYZER FOR CYANIDE-FREE REAGENTS
2. At the CBC module, remove the tubing between port 5 on the WBC bath and the
feed-through fitting FF27-1 and replace it with a 22 cm (8.5 in.) piece of the new
0.113 i.d. polyurethane tubing. See Figure 3.10-1.
3. Open the right-side door of the instrument to access the rear of the CBC module and the
Main Diluter module.
4. Disconnect and discard the Y-fitting FY8 and the tubing that connected FY8 to FF27-2.
Figure 3.10-1 Reconfiguring the Instrument for the Cyanide-Free Reagent System
Special
tubing
CBC
module
6 cm ( 2.25 in.)
0.113 i.d. tubing
2cm (0.75 in.)
2
0.113 i.d. tubing
1 3
Port 5
FF27
FY8 0.125-0.062 i.d. Existing
2 tubing
0.085 i.d. uniom fitting
1 Y-fitting
WBC
bath
22 cm (8.5 in.)
5961286D
0.113 i.d. tubing
ATTENTION: Ensure all tubing is installed correctly. If fittings are barbed, the tubing must cover
the barbs.
5. Attach new tubing to the replacement Y-fitting for FY8:
a. Connect one end of a 6 cm (2.25 in.) piece of the new 0.113 i.d. polyurethane
tubing to location 1 on the new 0.085 i.d. Y-fitting. Refer to Figure 3.10-1.
b. Connect one end of a 2 cm (0.75 in.) piece of the new 0.113 i.d. polyurethane
tubing to location 3 on the 0.085 i.d. Y-fitting, and connect the other end of the
tubing to the 0.125 i.d. end of the union fitting. Refer to Figure 3.10-1.
6. Install the new FY8 and associated tubing:
a. Connect the tubing attached to location 1 on FY8 to FF27-2. Refer to Figure 3.10-1
b. Connect the special tubing previously attached to location 2 on the old Y-fitting to
location 2 on the new Y-fitting.
c. Trim 0.5 cm (0.25 in.) from the tubing previously attached to location 3 on the old
Y-fitting and connect the tubing to the 0.062 i.d. end of the union fitting. Refer to
Figure 3.10-1.
3.10-2 PN 4235961E
7. Locate CK32 in the pneumatic line to the RBC diluent dispenser and replace the blue
choke with the 0.010 orifice black choke.
8. If you are doing this procedure as part of instrument installation, continue with the
installation procedure. Go to Heading 3.4, TESTING/CONFIGURING ASSEMBLIES.
If not, go to step 9.
9. Verify the instrument is working correctly before installing the new reagent system. Go
to Verification of the Hardware Changes.
Verification of the Hardware Changes

1. Do a Startup cycle (Main Menu tt Diluter Functions tt Start Up) and ensure:
a. The instrument passes all background checks.
b. No leaks occur within the Diluter system.
2. Close the instrument doors and check reproducibility in the Primary mode as directed in
Verifying Reproducibility in the Primary Mode under Heading 4.5, REPRODUCIBILITY
CHECKS.
3. Run controls and ensure the results fall within the expected ranges
4. Install the cyanide-free reagent system; go to the Reagent Changes procedure below.
Reagent Changes
Note: This procedure includes pre-conversion and post-conversion checks.
r The Pre-Conversion Instrument Verification ensures the instrument is calibrated and

working correctly before it is converted to the new reagent system.
r The Post-Conversion Instrument Verification ensures the instrument is calibrated and
working correctly after it is converted to the new cyanide-free reagent system.
B Clean paper towels
B 5C cell control, all levels
B A minimum of 20 whole-blood specimens less than 6 hours old
Pre-Conversion Instrument Verification

1. Do the CBC Latex Calibration and Verification procedure as directed under Heading 4.4,
LATEX CALIBRATION AND VERIFICATION. Make any necessary adjustments to the
aperture current voltages.
2. Do the Diff and Retic Latex Calibration and Verification procedure as directed under
Heading 4.4, LATEX CALIBRATION AND VERIFICATION. Make any necessary
adjustments to the RF and DC gains.
3. Do the LYTIC REAGENT AND DILUENT DELIVERY TIMING CHECK as directed
under Heading 4.20. Make any necessary modifications.
4. Do the reproducibility checks as directed under Heading 4.5, REPRODUCIBILITY
CHECKS. Ensure the results are within acceptable limits.
5. Do the carryover checks as directed under Heading 4.6, CARRYOVER CHECKS. Ensure
the results are within acceptable limits.
PN 4235961E 3.10-3
6. Run controls and verify the results fall within the expected ranges. If the controls do not
fall within the expected ranges, have the customer recalibrate the instrument according
to their laboratory’s protocol. Beckman Coulter recommends the use of S-CAL calibrator.
7. To establish a baseline of instrument performance, run 20 different, fresh (less than
6 hours old), whole-blood specimens using either of the following:
r The CBC Calibration screen (Special Functions tt Calibration tt CBC Calibration).
r The Reproducibility screen (Special Functions tt Calibration tt Reproducibility).
8. Print out the baseline results.
Reagent Conversion
1. Select the Disable Reagent Sensors screen (Main Menu tt Special Functions tt Diagnostics tt
Operator Options tt Fluidic Test tt Disable Reagent Sensors) and disable the LYSE and
DILUENT level sense alarms.
2. Place clean paper towels near the CBC lytic reagent container and the diluent container.
3. Being careful not to touch the reagent ends of the pickup tubes, remove the pickup tubes
from the CBC lytic reagent and diluent containers, tapping off any excess reagent into
the reagent containers, and place the pickup tubes on the clean paper towels.
4. Remove the CBC lytic reagent from the reagent lines:
a. From the Main menu of the DMS, select Diluter Functions tt Prime Reagents tt Lyse.
b. Press Û to initiate the Prime Lyse function.
c. Repeat the Prime Lyse function three more times.
5. Remove the diluent from the reagent lines:
a. From the Main menu of the DMS, select Diluter Functions tt Prime Reagents tt Diluent.
b. Press Û to initiate the Prime Diluent function.
c. Repeat the Prime Diluent function three more times.
6. Carefully install the pickup tubes in the CBC lytic reagent and diluent containers of the
new, cyanide-free reagent system.
7. Record the new reagent information:
a. From the Main menu of the DMS, select Special Functions tt Setup tt System Setup tt
Reagents.
b. Enter the lot numbers and expirations dates for the CBC lytic reagent and diluent.
c. Press Ò to save the changes and exit the screen.
8. Repeat step 4 to prime the CBC lytic reagent lines.
9. Repeat step 5 to prime the diluent lines.
Post-Conversion Instrument Verification

1. With the upper front door closed, do a Startup cycle (Main Menu tt Diluter Functions tt
Start Up) and verify all startup results Pass.
2. Do the CBC Latex Calibration and Verification procedure as directed under Heading 4.4,
LATEX CALIBRATION AND VERIFICATION.
3.10-4 PN 4235961E
3. Do the Diff and Retic Latex Calibration and Verification procedure as directed under
4. Using either the CBC Calibration or the Reproducibility screen, rerun the same 20
whole-blood specimens you ran in step 7 of the Pre-Conversion Instrument Verification
and print out the results.
5. Compare the parameter mean values on the post-conversion printout to the parameter
mean values on the baseline (pre-conversion) printout, and verify the values fall within
the mode-to-mode limits specified in Table A.1-12, Secondary Mode-to-Primary Mode
Comparison Limits.
r If the post-conversion results are not within the limits, troubleshoot the problem
and repeat from step 4.
r If the post-conversion results are within the limits, have the customer determine if
the results are acceptable.
6. If the post-conversion results are within the mode-to mode limits but are not acceptable
to the customer, have the customer recalibrate the instrument.
7. Instruct the customer to:
a. Set up control files using the assigned values for the new cyanide-free reagent
system.
b. Run their controls and ensure the results fall within the expected ranges.
PN 4235961E 3.10-5
3.10-6 PN 4235961E
CONTENTS
4
4 SERVICE AND REPAIR PROCEDURES, 4.1-1
4.1 GUIDELINES FOR SERVICING THE MAXM ANALYZER, 4.1-1

General, 4.1-1
Power Down/Power Up the System, 4.1-2
Purpose, 4.1-2
Power Down, 4.1-2
Power Up, 4.1-2
Reset the System, 4.1-2
Clear the CPU RAM, 4.1-3
Purpose, 4.1-3
Procedure, 4.1-3
4.2 USING THE SERVICE DISK, 4.2-1

Purpose, 4.2-1
Activating the DMS Service Options, 4.2-1
Deactivating the DMS Service Options, 4.2-2
Using the Service Disk Software on a DMS, 4.2-3
Using the Service Disk Software on a Hard Drive, 4.2-5
Installing the Software, 4.2-5
Running the Software, 4.2-5
4.3 WHOLE BLOOD VERIFICATION - CBC/DIFF, 4.3-1

Purpose, 4.3-1
Procedure, 4.3-1
4.4 LATEX CALIBRATION AND VERIFICATION, 4.4-1

Purpose, 4.4-1
CBC Latex Calibration and Verification, 4.4-1
Procedure, 4.4-1
Diff and Retic Latex Calibration and Verification, 4.4-2
Procedure, 4.4-2
4.5 REPRODUCIBILITY CHECKS, 4.5-1

Purpose, 4.5-1
Verifying Reproducibility in the Primary Mode, 4.5-1
Procedure, 4.5-1
Verifying Reproducibility in the Secondary Mode, 4.5-2
Procedure, 4.5-2
Verifying Retic % Reproducibility (Sample Analysis), 4.5-3
Procedure, 4.5-3
4.6 CARRYOVER CHECKS, 4.6-1

Purpose, 4.6-1
Checking Carryover in the Primary Mode, 4.6-1
PN 4235961E 4-i
CONTENTS

Procedure, 4.6-1
Checking Carryover in the Secondary Mode, 4.6-2
Procedure, 4.6-2
Retic Carryover Tests, 4.6-2
General Instructions, 4.6-3
Retic Mode-to-Mode Carryover, 4.6-3
Retic Within Mode, 4.6-3
4.7 INITIAL ADJUSTMENT OF PRIMARY-MODE CALIBRATION FACTORS, 4.7-1

Purpose, 4.7-1
Procedures, 4.7-1
4.8 SECONDARY MODE-TO-PRIMARY MODE CALIBRATION, 4.8-1

Purpose, 4.8-1
Procedure, 4.8-1
4.9 VCS OPTIMIZATION, 4.9-1

Purpose, 4.9-1
A. Preliminary Checks, 4.9-3
B. Count Ratio Check, 4.9-3
C. VCS Flow-Rate Adjustment, 4.9-4
D. Clog Detector Circuit Adjustment, 4.9-4
E. RF Detector Preamp Card C1 Adjustments, 4.9-4
F. DC, RF, and LS Gains Adjustments, 4.9-4
G. DC, RF and LS Noise Measurements, 4.9-4
H. Erythrolyse II and StabiLyse Reagent Pumps Volume Adjustments, 4.9-4
I. Verification, 4.9-4
4.10 VCS FLOW-RATE ADJUSTMENT, 4.10-1

Purpose, 4.10-1
Sample Pressure Adjustment, 4.10-1
4.11 RF DETECTOR PREAMP CARD ADJUSTMENTS, 4.11-1

Purpose, 4.11-1
Clog Detector Circuit Adjustment, 4.11-1
Procedure, 4.11-1
C1 Adjustment, 4.11-2
Procedure, 4.11-2
4-ii PN 4235961E
CONTENTS
4
4.12 RMS NOISE CHECKS, 4.12-1
Purpose, 4.12-1
CBC RMS Noise Checks, 4.12-1
Procedure, 4.12-1
Diff and Retic RMS Noise Checks, 4.12-1
Procedure, 4.12-1
4.13 FLOW-CELL CLEANING, 4.13-1

Purpose, 4.13-1
Procedure, 4.13-1
4.14 LENS BLOCK CLEANING, 4.14-1

Purpose, 4.14-1
Procedure, 4.14-1
4.15 LASER/FLOW CELL ALIGNMENT, 4.15-1

Purpose, 4.15-1
Flow-Cell Cleaning, 4.15-2
Flow-Cell Z-Axis (Focus) Alignment, 4.15-2
Flow-Cell X- and Y-Axis Plate Alignment, 4.15-4
Flow-Cell Y-Axis Final Alignment, 4.15-6

REPLACEMENT, 4.16-1
Erythrolyse II and StabiLyse Reagent Pumps Adjustment, 4.16-1
Purpose, 4.16-1
Adjusting the Erythrolyse II Reagent Pumps Volume, 4.16-4
Adjusting the StabiLyse Reagent Pump Volume, 4.16-5
Optimizing the Erythrolyse II Reagent Pumps Volume, 4.16-7
Verifying Erythrolyse II and StabiLyse Reagent Pumps Volume
Adjustment, 4.16-8
Erythrolyse II Reagent Pumps Replacement, 4.16-8
Erythrolyse II Reagent Pumps Removal, 4.16-8
Erythrolyse II Reagent Pump Installation, 4.16-8
4.17 ASPIRATION PUMP VOLUME ADJUSTMENT, 4.17-1

Procedure, 4.17-1
PN 4235961E 4-iii
CONTENTS
4.18 CBC LYTIC REAGENT PUMPS VERIFICATION, 4.18-1

Procedure, 4.18-1
4.19 RBC/WBC DILUENT DISPENSERS VOLUME TESTING, 4.19-1

Purpose, 4.19-1
Measuring Diluent by Volume, 4.19-1
Procedure, 4.19-1
Measuring Diluent by Weight, 4.19-2
Procedure, 4.19-2
4.20 LYTIC REAGENT AND DILUENT DELIVERY TIMING CHECK, 4.20-1

Purpose, 4.20-1
Procedure, 4.20-1
4.21 BLOOD/BUBBLE DETECTORS GAIN ADJUSTMENT, 4.21-1

Procedure, 4.21-1
4.22 ELECTRONIC POWER SUPPLY REPLACEMENT, 4.22-1

Removal, 4.22-1
4.23 VOLTAGE CHECKS AND ADJUSTMENTS, 4.23-1

System Voltage Checks, 4.23-1
Using the DMS to Check System Voltages, 4.23-1
Using an External DMM to Check and Adjust System Voltages, 4.23-1
Laser On Current/LS Offset Voltage Check, 4.23-3
Procedure, 4.23-3
System Voltage Checks Verification, 4.23-4
4.24 PNEUMATIC POWER SUPPLY REPLACEMENT, 4.24-1

Removal, 4.24-1
Setup of Pneumatic Power Supply Buck-Boost Transformer, 4.24-1
4.25 PRESSURE/VACUUM ADJUSTMENT, 4.25-1

General, 4.25-1
System Test (Pressure/Vacuum Verification), 4.25-1
Pressure Adjustments, 4.25-1
4-iv PN 4235961E
CONTENTS
4
Initial Sample Pressure Adjustment, 4.25-5
Vacuum Adjustments, 4.25-5
High Vacuum Verification, 4.25-5
Low Vacuum Adjustment, 4.25-6
4.26 SOLENOID VALVES INSPECTION AND REPLACEMENT, 4.26-1

Solenoid Inspection, 4.26-1
Solenoid Replacement, 4.26-6
Removal, 4.26-6
4.27 HEMOGLOBIN PREAMP MODULE REPLACEMENT, 4.27-1

Purpose, 4.27-1
Removal, 4.27-1
Hemoglobin Lamp Adjustment, 4.27-2
4.28 APERTURE MODULE AND BATH ASSEMBLY, 4.28-1

Removal, 4.28-1
4.29 BSV AND BSV HOUSING/ACTUATOR DISASSEMBLY/REPLACEMENT, 4.29-1

Purpose, 4.29-1
BSV Disassembly/Replacement, 4.29-1
Purpose, 4.29-1
BSV Removal, 4.29-1
BSV Installation, 4.29-3
BSV Disassembly/Replacement Verification, 4.29-4
BSV Housing Replacement, 4.29-5
Purpose, 4.29-5
BSV Housing Removal, 4.29-5
BSV Housing Installation, 4.29-5
BSV Actuator Replacement, 4.29-6
Purpose, 4.29-6
BSV Actuator Removal, 4.29-6
BSV Actuator Installation, 4.29-7
BSV Alignment, 4.29-9
BSV and BSV Housing Verification, 4.29-11
Purpose, 4.29-11
Procedure, 4.29-11
PN 4235961E 4-v
CONTENTS
4.30 CAROUSEL MOTOR REPLACEMENT, 4.30-1

Removal, 4.30-1
4.31 CODE WHEEL ALIGNMENT, 4.31-1

Procedure, 4.31-1
4.32 NEEDLE REPLACEMENT, 4.32-1

Needle Replacement - MAXM Analyzer with Autoloader Module, 4.32-1
Purpose, 4.32-1
Procedure, 4.32-1
Needle Replacement - MAXM Analyzer with Rotary Cap-Pierce Module, 4.32-5
Purpose, 4.32-5
Procedure, 4.32-5
4.33 RINSE BLOCK ADJUSTMENT, 4.33-1

Procedure, 4.33-1
4.34 AUTOLOADER MODULE REMOVAL, 4.34-1

Purpose, 4.34-1
Removal, 4.34-1
4.35 ROCKER BED ASSEMBLY REMOVAL, 4.35-1

Purpose, 4.35-1
Removal, 4.35-1
4.36 ELEVATOR PLATFORM STEPPER MOTOR REPLACEMENT, 4.36-1

Purpose, 4.36-1
Removal, 4.36-1
4.37 TUBE AVAILABLE SENSOR ASSEMBLY REPLACEMENT, 4.37-1

Purpose, 4.37-1
Removal, 4.37-1
4-vi PN 4235961E
CONTENTS
4
4.38 BAR-CODE READER DECODER CARD CONFIGURATION, 4.38-1

Purpose, 4.38-1
Procedure, 4.38-1
Selecting the Settings for the Microscan™ Bar-Code Reader Decoder
Card, 4.38-2
4.39 BAR-CODE SCANNER ALIGNMENT (FOR AUTOLOADER MODULE

ONLY), 4.39-1
Purpose, 4.39-1
Procedure, 4.39-1
Aligning the Bar-Code Scanner, 4.39-2
4.40 ROCKER BED LINKAGE ADJUSTMENT, 4.40-1

General, 4.40-1
Procedure, 4.40-2
4.41 SPECIMEN TUBE STOPPER PIERCE-PROXIMITY ADJUSTMENT, 4.41-1

General, 4.41-1
Purpose, 4.41-1
Preliminary Procedure, 4.41-2
Horizontal Pierce-Proximity Adjustments, 4.41-2
Vertical Pierce-Proximity Adjustments, 4.41-2
4.42 CASSETTE INDEX MOTOR/INDEX HUB GAP ADJUSTMENT, 4.42-1

Purpose, 4.42-1
Procedure, 4.42-1
4.43 SWITCH/SENSOR CHECK, 4.43-1

Purpose, 4.43-1
Procedure, 4.43-1
PN 4235961E 4-vii
CONTENTS
4.44 NEEDLE-POSITION SENSORS ADJUSTMENT, 4.44-1

Purpose, 4.44-1
Preliminary Check, 4.44-1
Adjustment, 4.44-2
ILLUSTRATIONS
4.1-1 Switches for Clearing the CPU RAM, 4.1-3
4.2-1 Control and Sample Files Available for Viewing, 4.2-4

4.2-2 Single-Parameter Graph, 4.2-5
4.2-3 Parameter vs. Parameter Graph, 4.2-6
4.2-4 Multiple-Parameter Graph, 4.2-6
4.2-5 Flag Graph, 4.2-6
4.2-6 Statistics Window, 4.2-7
4.2-7 Sequential (Points) Mode, 4.2-7
4.2-8 Zoom Mode, 4.2-7
4.2-9 Histogram Mode, 4.2-8
4.2-10 Date-Offset Mode, 4.2-8
4.2-11 Control-File Graph, 4.2-8
4.2-12 Service Disk Utilities Menu Tree, 4.2-9
4.9-1 Optimization Procedure Flow, 4.9-1

4.9-2 Histogram of Good Count Ratio Data, 4.9-3
4.9-3 Histogram of Poor Count Ratio Data, 4.9-3
4.10-1 Unacceptable Flow-Rate Graph, 4.10-2
4.13-1 Wetting Applicator with Alcohol, 4.13-2

4.13-2 Wiping Front of Flow Cell, 4.13-2
4.13-3 Wiping Rear of Flow Cell, 4.13-2
4.15-1 Laser/Flow-Cell Alignment Work Flow, 4.15-1

4.15-2 Flow-Cell Z-Axis Alignment, 4.15-3
4.15-3 Scatter Sensor Laser Diffraction, 4.15-4
4.15-4 Flow-Cell X- and Y-Axis Plate Alignment, 4.15-5
4.15-5 Laser Reflected Light Beam with Special Light Shield Installed, 4.15-6
4.15-6 Laser Diffraction Pattern on Paper, 4.15-7
4.16-1 Match Sheath Fluid to Sample Stream Conductivity, 4.16-1

4.16-2 Effect of Dilution Conductivity on Count Ratio, 4.16-2
4.16-3 Effect of Osmolality on NE DC mean, 4.16-2
4.16-4 Erythrolyse II Reagent and StabiLyse Reagent Pump Optimization Flow
Diagram, 4.16-3
4.16-5 Tubing from Mixing Chamber, 4.16-4
4.16-6 Volumetric Cylinder Calibration Marks, 4.16-4
4.16-7 Reading the Meniscus, 4.16-5
4.16-8 Diaphragm Pump Adjustment, 4.16-5
4.16-9 Pump Module Mounting Bracket, Old Configuration, 4.16-8
4-viii PN 4235961E
CONTENTS
4
4.16-10Pump Module Mounting Bracket, New Configuration, 4.16-8
4.17-1 Second Pull (Vacuum), 4.17-1

4.17-2 First Pull (Aspiration Pump)), 4.17-2
4.19-1 Reading a Meniscus, 4.19-2

4.20-1 WBC Bath Tubing Disconnect for Lytic Reagent/Diluent Delivery Timing
Hookup, 4.20-2
4.20-2 Test Box and Transducer Hookup for Lytic Reagent/Diluent Timing
Procedure, 4.20-2
4.20-3 Digital Pressure Meter Hookup for Lytic Reagent/Diluent Timing
Procedure, 4.20-3
4.21-1 Blood/Bubble Detector Test Points and Adjustments, 4.21-1
4.22-1 Electronic Power Supply Connectors, 4.22-1

4.23-2 Electronic Power Supply, Top View with Cover Removed, 4.23-2
4.24-1 Pneumatic Supply Removal, 4.24-1
4.25-1 60-psi Pressure Regulator, Old Configuration, 4.25-2

4.25-2 60-psi Pressure Regulator, New Configuration, 4.25-2
4.27-1 Hgb Preamp Module, 4.27-1
4.28-1 Aperture Module and Bath Assembly, 4.28-1
4.29-1 Retaining the Probe-Slide Mechanism, 4.29-1

4.29-2 Removing the Rinse Block Screw and Washer, 4.29-2
4.29-3 Moving the Air Cylinder, 4.29-2
4.29-4 Aligning the Air Cylinder, 4.29-3
4.29-5 Replacing the Rinse Block, 4.29-4
4.29-6 BSV Actuator (Configuration A) Allen Screw and Cylinder Seals, 4.29-6
4.29-7 BSV Actuator Removal, Configuration A, 4.29-7
4.29-8 BSV Actuator Removal, Configuration B, 4.29-7
4.29-9 Aligning the BSV Housing, 4.29-9
4.29-10Aspiration First Pull (Aspiration Pump), 4.29-11
4.29-11Aspiration Second Pull (Vacuum), 4.29-12
4.30-1 Carousel Motor and Carousel Assembly, 4.30-1
4.31-1 Code Wheel, 4.31-2
4.32-1 Cleaning Solution, 4.32-1

4.32-2 Cassette into Loading Bay, 4.32-1
4.32-3 Safety Clip Installation, 4.32-2
4.32-4 Needle Cartridge Removal, 4.32-2
4.32-5 Needle Assembly Location Grooves, 4.32-3
PN 4235961E 4-ix
CONTENTS
4.32-6 Needle Assembly Tubing, 4.32-3

4.32-7 Needle Tubing Quick Disconnects, 4.32-4
4.32-8 Needle Assembly Installation, 4.32-4
4.32-9 Safety Clip Removal, 4.32-4
4.32-10Cleaning Solution, 4.32-5
4.32-11Exit Tray Removal, 4.32-6
4.32-12Needle Tubing Quick Disconnects, 4.32-6
4.32-13LOCK Lever, 4.32-6
4.32-14Needle Assembly Removal, 4.32-7
4.32-15Needle Assembly Orientation, 4.32-7
4.32-16Needle Assembly Alignment, 4.32-7
4.32-17Slots, 4.32-8
4.32-18Needle Assembly Installation, 4.32-8
4.32-19Needle Assembly Quick Disconnects, 4.32-8
4.32-20Exit Tray Reinstallation, 4.32-9
4.33-1 Rinse Block Adjusting Screw, Old BSV Configuration, 4.33-1

4.33-2 Rinse Block Adjusting Screw, New BSV Configuration, 4.33-2
4.34-1 Autoloader Module Guide Rails, 4.34-2

4.34-2 Autoloader Module Removal, 4.34-2
4.35-1 Rocker Bed Removal, 4.35-1

4.35-2 Rocker Bed Hardware, 4.35-2
4.39-1 Bar-Code Scanner Adjustment Screws, 4.39-2
4.40-1 Rocker Bed Linkage, 4.40-1

4.40-2 Rocker Bed Linkage Adjustment, 4.40-2
4.41-1 Specimen Tube Stopper Piercing Proximity, 4.41-1
4.42-1 Index Motor/Index Hub Gap Adjustment, 4.42-1
4.44-1 Needle-Position Sensor Orientation on the Needle Drive Cylinder, 4.44-1
TABLES
4.2-1 DMS Service Menu Options, 4.2-2
4.2-2 Service Disk Utilities Selections/Explanations, 4.2-10
4.9-1 Purpose of VCS Optimization Procedures, 4.9-2
4.26-1 Solenoid Operations, 4.26-1
4.38-1 Moving Around the Microscan Screens, 4.38-3
4.43-1 Switch/Sensor Checks on the MAXM Analyzer with Autoloader Module, 4.43-1
4.43-2 Switch/Sensor Checks on the MAXM Analyzer with Rotary Cap-Pierce
Module, 4.43-5
4-x PN 4235961E
4SERVICE AND REPAIR PROCEDURES 4
4.1 GUIDELINES FOR SERVICING THE MAXM ANALYZER
General
1. Review and heed the general safety warnings and cautions listed under Heading 1.2,
SAFETY PRECAUTIONS.
2. Ensure there is adequate space to work and to access the instrument components safely.
Note: You can remove the CBC, BSV and Pump modules from the front of the Diluter, or
reposition them, to more easily access components.
3. Before removing panels to access electronic components, power down the instrument.
See Power Down/Power Up the System in this section.
CAUTION Risk of damage to electronic components. Removing or replacing a circuit card or other
electronic component while the power is ON can damage the component. To prevent damage to circuit
cards and other electronic components, always turn off the power before removing or replacing the
component.
4. Before disconnecting or reconnecting any electronic component (including solenoid

valves), turn off the instrument. See Power Down/Power Up the System in this section.
5. To keep the power on but ensure the MAXM analyzer will not cycle while you are
servicing it, or to keep the power on but turn the Pneumatic Power Supply off, set the
Standby/Reset switch to Standby (0).
6. To operate the instrument with the lower front door open, use a magnet to bypass the
lower door switch.
Note: Use the magnet connected to the lower door if a separate magnet is not available.
7. To avoid electronic noise when operating the MAXM analyzer with the upper front door
open, install the electromagnetic shield over the sensing area in the CBC module.
8. When resetting the MAXM analyzer, be sure to reset the entire system. See Reset the
System in this section.
Note: Because the DMS controls and monitors all the MAXM analyzer functions, for
correct instrument operation the DMS must maintain constant communication with the
Main Unit. Resetting either the DMS or the Main Unit individually instead of resetting
the entire system could interrupt that communication.
9. To clear the CPU RAM so that the DMS can reload the software, do the Clear the CPU
RAM procedure at the end of this section.
10. You can do most of the procedures in this chapter using the tools in a standard Service
Tool Kit, a digital voltmeter, and an oscilloscope. Any special tools or equipment and any
supplies needed are listed under “Tools/Supplies Needed” at the beginning of the
procedure.
PN 4235961E 4.1-1
SERVICE AND REPAIR PROCEDURES
GUIDELINES FOR SERVICING THE MAXM ANALYZER
11. Unless otherwise noted, the procedures in this chapter are presented as if you are looking
at the front of the MAXM analyzer.
12. When you have finished servicing the instrument, always verify instrument performance
as follows:
a. On instruments without a pressure tank, check the 30 and 60 psi as directed under
Heading 4.25, PRESSURE/VACUUM ADJUSTMENT.
b. On instruments with a BSV that uses air cylinders, do the BSV and BSV Housing
Verification procedure as directed under Heading 4.29, BSV AND BSV
HOUSING/ACTUATOR DISASSEMBLY/REPLACEMENT.
c. Do a Start Up or System Test.
d. Check reproducibility as instructed under Heading 4.5, REPRODUCIBILITY
CHECKS.
e. Run controls.
Power Down/Power Up the System
Purpose
Following the Power Down procedure ensures all power is removed from the instrument,
preventing personal injury from electric shock.
Power Down
ATTENTION: Always place the MAXM analyzer in Standby before turning off the power at the
main POWER switch. Selecting Standby puts the instrument through a controlled power down
sequence that ensures the battery-protected memory is protected.
1. Set the Standby/Reset switch to Standby (O).
2. Turn OFF the POWER switch on the rear of the Electronic Power Supply.
3. Unplug the ac power cord from the ac wall outlet.
Power Up
1. Plug the ac power cord into the ac wall outlet.
2. Turn ON the POWER switch on the rear of the Electronic Power Supply.
3. Set the Standby/Reset switch to Ready (I).
Reset the System

1. Set the Standby/Reset switch to Standby (O).
2. Wait at least 15 seconds.
Note: Waiting allows the compressor head to partially bleed off pressure.
3. Set the Standby/Reset switch to Ready (I).
4.1-2 PN 4235961E
GUIDELINES FOR SERVICING THE MAXM ANALYZER 4
Clear the CPU RAM
Purpose
Clearing the CPU RAM allows the DMS to reload the instrument software.
Procedure
1. Open the upper front door of the Main Unit.
2. Simultaneously press the RESET button on the 376 CPU card and the sample bar
(whole-blood switch). See Figure 4.1-1.
Figure 4.1-1 Switches for Clearing the CPU RAM

376 CPU
card
SCAN
SCAN
LED
RESET Sample
RESET bar
button
5961200D
3. Release the RESET button, but continue to press the sample bar. The CPU SCAN LED
goes off and then on.
4. When the CPU SCAN LED goes off a second time release the sample bar.
5. If the CPU SCAN LED remains off the RAM is erased. Erasing the RAM enables the DMS
to reload the instrument software.
PN 4235961E 4.1-3
GUIDELINES FOR SERVICING THE MAXM ANALYZER
4.1-4 PN 4235961E
USING THE SERVICE DISK 4
4.2 USING THE SERVICE DISK
Purpose
This procedure explains how to correctly use the Service Disk 2 and is valid for all series of
personal computers on the MAXM analyzer. Use this procedure whenever you need to:
r Access one of the DMS service options or one of the Service Disk 2 software options to
perform a troubleshooting, alignment, verification or calibration procedure on
the instrument.
Note: See Heading A.6, SOFTWARE MENU TREES for illustrations of all the MAXM
analyzer software menu trees.
r Display an enhanced printout for a sample.
The original Service Disk was simply a "key" to open the door to the DMS service options in
the DMS program software. The Service Disk 2, however, also contains software for diagnostic
programs, tools to help you locate instrument problems.
The procedures under Activating the DMS Service Options show you how to use the Service
Disk as a “key” to the DMS service options. The procedures under Using the Service Disk
Software on a DMS show you how to access the software options on Service Disk, and the
procedures under Using the Service Disk Software on a Hard Drive show you how to install
those software options onto the hard drive of your laptop or personal computer.
Use the Deactivating the DMS Service Options procedure when you have completed your
tasks and be very careful not to leave the disk at a customer account.
B Service Disk 2, PN 6415262(3.5 in.)
Activating the DMS Service Options

1. Ensure the instrument is ON.
2. Insert the Service Disk into drive A.
Note: There is no prompt to advise you to insert the disk.
3. From the Main menu on the DMS, select Special Functions tt Diagnostics tt Service Options.
ATTENTION: The Service Disk must be in the drive bay while you type the password.
4. Type the password SERVICE, then press Û. The Service Options menu appears on the
DMS screen. The options available on the menus are described in Table 4.2-1.
5. To select one of the service options, highlight the option and press Û.
Note: To select a service option with fewer key strokes, press the highlighted "hot key."
6. To display the enhanced printout of a sample:
a. Press Ñ to return to the Main menu.
b. Select Sample Analysis tt Run Samples.
7. To access the WBC histograms:
a. Press Ñ to return to the Main menu.
PN 4235961E 4.2-1
USING THE SERVICE DISK
b. Select Sample Analysis tt Run Samples.

c. Press Î then Þ+Î to display the average histograms for both RBC and WBC.
d. Press Ï then Þ+É, Þ+Ê, and Þ+Ë to display the single-aperture
histograms for both RBC and WBC.
Deactivating the DMS Service Options

ATTENTION: Always deactivate the service options after you complete your tasks.
1. Remove the Service Disk from drive A.

2. Turn the DMS OFF then ON to reboot the computer and deactivate the DMS
service options.
Table 4.2-1 DMS Service Menu Options
Menu Options Description

Certification Allows access to routines for determining the reproducibility and carryover results in
the Primary, Secondary, and Retic modes.
Latex Checks Allows you to run latex particles and display the data for various test and adjustments.
The options available are:
r Red & White Channel Calibration - used for calibrating the aperture current
voltage in the CBC mode.
r MPV Latex - not currently used.
r Diff Latex - used for adjusting the LS, DC and RF gains in the Diff mode.
r Retic Latex - used for adjusting the LS, DC and RF gain settings in the Retic mode.
WBC Histogram Check Prints the WBC histogram, with an overlay check, from the last transmitted results.
Switch/Sensor Check Provides a screen for monitoring the status of the switches and sensors in the Main
Unit. For the procedure, see Heading 4.43, SWITCH/SENSOR CHECK.
Test Pulses Not available on the MAXM analyzer.
Enter Secondary Cal Factors Allows you to manually enter the Secondary-mode calibration factors.
Init Cal Factors Resets the Cal factors to a default setting of 1.000 in either the Primary or the
Secondary mode.
Solenoid Test Allows you to energize one solenoid or to select and energize a group of solenoids
simultaneously. In this function a solenoid remain energized until you de-energize it or
for 10 minutes, whichever comes first. For the procedure, see Heading 4.26,
SOLENOID VALVES INSPECTION AND REPLACEMENT.
Also allows access to the following Í Cycles:
r Erythrolyse Pump Dispense - activates the Erythrolyse II reagent pump once for
checking operation of the pump or for priming the Erythrolyse II reagent lines.
r StabiLyse Pump Dispense - activates the StabiLyse reagent pump once for
checking operation of the pump or for priming the StabiLyse reagent lines.
r Erythrolyse Pump Volume Test (X5) - activates the Erythrolyse II reagent pump
five times for verifying the volume dispensed.
r StabiLyse Pump Volume Test (X7) - activates the StabiLyse reagent pump seven
times for verifying the volume dispensed.
4.2-2 PN 4235961E
Table 4.2-1 DMS Service Menu Options
Menu Options Description

Raw Data Allows you to enable the raw data capture option. When this option is enabled, the raw
differential data from subsequent samples is saved to the hard drive so that it can be
down loaded to an external drive. A Technical Support representative would generate
the request for this raw data.
Secondary Mode Cal Accesses a routine for establishing calibration factors for the Secondary mode.
Autoloader Tests Allows you to access four diagnostic routines for testing the Autoloader module:
r Autoloader Test Routine - does an entire Autoloader module cycle from taking a
cassette out of the loading bay to placing the cassette in the unloading bay. Used
to check the function of the Autoloader module.
r One Position Cassette Advance - advances a cassette on the rocker bed to the
next cassette position sensor.
r Needle Pierce Test - moves the cassette to the tube available sensor, pierces the
tube, and removes the needle from the tube. Used to check the piercing function
and needle alignment.
r Lift and Load/Unload -picks up a cassette in the loading bay, moves the cassette
across the rocker bed, and places the cassette in the unloading bay. Used to check
the loading and unloading functions.
Cassette and Position Number Displays the instrument configuration for the number of positions in a cassette.
Using the Service Disk Software on a DMS

ATTENTION: Use this procedure each time you need to access the Service Disk software
options on a DMS. Do not install the Service Disk software onto the hard drive of the DMS.
1. Insert the Service Disk onto drive A.
2. Turn the DMS OFF then ON to reboot the computer.
3. When the prompt Only control files? appears, to access:
r Control file data only, press Y then press Û. The control files are the files with
the prefix 5CII, Latron or Retic-C.
Note: It is much faster to display only the control files.
r Sample data or sample and control data, press N then press Û. The files you can
view with this option are shown in Figure 4.2-1.
4. To graph one or more of the parameter options displayed on the Main Screen:
a. Using è, é, æ, and ç, highlight the desired option and then press ß to
“tag” the option. You can tag up to four options.
Note: Figures 4.2-2 through 4.2-5 are examples of the types of graphs available. A
description accompanies each figure.
b. Press Û.
Note: The tagged graphs are displayed simultaneously.
5. To enlarge a graph to full size, press Ù until the box around the graph is highlighted,
then press Û.
6. To determine the functions of the F-keys for the displayed graph, press É.
PN 4235961E 4.2-3
7. To determine the statistics for the selected graph (Figure 4.2-6), press Ë.
Figure 4.2-1 Control and Sample Files Available for Viewing

CBC: WBC Results FLAG: Blasts DIFF: DF2 Summary
CBC: WBC (all apertures) FLAG: Imm Grans DIFF: Count Ratio
CBC: RBC Results FLAG: Variant Lymphs DIFF: Flow Rate
CBC: RBC (all apertures) DIFF: LY% Values 5CII: NE DC Mean
CBC: HGB Results DIFF: MO% Values 5CII: NE DC SD
CBC: HCT Results DIFF: NE% Values 5CII: NE OP Mean
CBC: MCV Results DIFF: EO% Values 5CII: NE OP SD
CBC: MCV (all apertures) DIFF: BA% Values 5CII: NE RLS Mean
CBC: MCH Results DIFF: LY# Values 5CII: NE RLS SD
CBC: MCHC Results DIFF: MO# Values 5CII: NE PVR
CBC: RDW Results DIFF: NE# Values Latron: Volume
CBC: PLT Results DIFF: EO# Values Latron: Conductivity
CBC: PLT (all apertures) DIFF: BA# Values Latron: Scatter
CBC: MPV Results DIFF: NE DC Mean Latron: Retic Volume
CBC: MPV (all apertures) DIFF: NE DC SD Latron: Retic Conductivity
CBC: H & H (HCT&HGB*3) DIFF: NE OP Mean Latron: Retic Scatter
FLAG: Partial Aspiration DIFF: NE OP SD RETIC: Retic %
FLAG: HGB Incomplete DIFF: NE RLS Mean RETIC: Retic #
FLAG: Flowcell Full Clog DIFF: NE RLS SD RETIC: Retic + RBC
FLAG: Flowcell PC2 DIFF: NE PVR Retic-C: Retic%
FLAG: Flowcell PC1 DIFF: NE Pop Dist
[ESC] - Menu DIFF: DF1 Summary
8. To change a single-parameter graph from Sequential (Points) mode (Figure 4.2-7) to an

alternate graph mode, use the F-keys indicated below.
Note: Use the same F-key to deselect the mode and return to the Sequential mode.
a. To select Zoom mode (Figure 4.2-8) press Ì. In this mode -
r To scroll the selector bar through the points, use è or é.
r To accelerate the selector bar, use Ý+è or Ý+é.
b. To select Histogram mode (Figure 4.2-9) press Í.
c. To select Date Offset mode (Figure 4.2-10) press Î. In this mode, to scroll
through the data points -
1) Press Ì to select the Zoom mode.
2) Use è or é to move the selector bar, use Ý+è or Ý+é to accelerate the
selector bar.
9. To change a control-file display (Figure 4.2-11) to another control file, use æ and .è
Note: You can only select control files that have data for the parameter selected.
10. To access the Service Disk utilities, press È. Figure 4.2-12 is a menu tree of the utility
options, Heading 4.2-2 describes the options and procedures for using the utility
options.
11. When you have completed your task, remove Service Disk from the DMS and turn the
DMS OFF then ON again to reboot the computer.
4.2-4 PN 4235961E
Using the Service Disk Software on a Hard Drive
Installing the Software

ATTENTION: Use this procedure to install the Service Disk software onto the hard drive of your
personal computer or laptop only. Do not install the Service Disk software onto the hard drive
of a DMS.
1. Insert the Service Disk into drive A.
2. At the A:> prompt, type INSTALL and press Û.
Note: This batch file creates a directory on your C drive and copies the Service Disk
utilities to this subdirectory.
Running the Software

ATTENTION: Your computer must have at least 485 K of conventional memory for all the
functions to work properly.
1. At the C:> prompt, type CD\DMSGR and press Û.
2. At the C:\DMSGR> prompt, type DMSGRAPH and press Û.
Note: In addition to using the utilities and viewing the graphs as described under Using
the Service Disk Software on a DMS, you can now retrieve and store multiple captured
databases on your personal computer or laptop.
3. Select Exit to exit this software and return to the DOS prompt.
Figure 4.2-2 Single-Parameter Graph On a single-parameter graph the Y-axis represents

value and the X-axis represents the sequence of the
data collection. Each plot point represents a single
value.
Examples of single-parameter graphs are:
r WBC Result
r Hgb Blank Voltage
r Count Ratio.
Single-parameter graphs can also be the result of a
function of one or more parameters, such as flow rate
where the Y-axis represents WBC result x time.
Single-parameter graphs can be displayed in Zoom,

Histogram and Date Offset mode.
PN 4235961E 4.2-5
Figure 4.2-3 Parameter vs. Parameter Graph On a parameter vs. parameter graph, any single
parameter is plotted on the X-axis vs. any other single
parameter on the Y-axis.
An example of this is the H & H graph where the Hct

result is plotted against 3 x the Hgb result.
Figure 4.2-4 Multiple-Parameter Graph On a multi-parameter graph, up to four parameters

are plotted simultaneously. Each parameter is plotted
in a different color: yellow, green, red or white.
Multi-parameter graphs are built from

single-parameter or parameter vs. parameter plots.
The following are built from single-parameter plots:
r RBC Result (all apertures)

r MCV Result (all apertures).
The DF1 Summary graph, on the other hand, is built
from three parameter vs. parameter plots: LY DC
Mean vs. LY RLS Mean, MO DC Mean vs. MO RLS
Mean and NE DC Mean vs. NE RLS Mean.
Figure 4.2-5 Flag Graph On a flag graph the Y-axis represents the number of
data points, the X-axis represents the state of the flag.
On all flag graphs except the one for the Imm Grans
flag, there are two channels (states) on the X-axis.
r The left channel is OFF (no flag)

r The right channel is ON.
On the Imm Grans flag graph, there are three
channels (states) on the X-axis.
r The left channel is OFF (no flag)

r The middle channel is Imm Grans 1
r The right channel is Imm Grans 2.
4.2-6 PN 4235961E
Figure 4.2-6 Statistics Window The statistics window shows the number of records
in the database or control file, the number of points
used for building the graph, and the Min, Max, Mean,
Mode, SD (standard deviation) and CV for the
displayed points.
Not all fields are in all records. For example, a CBC

Only mode sample does not have a Count Ratio. The
points used to build the graph are less than the total
points in the database.
Figure 4.2-7 Sequential (Points) Mode The Sequential (Points) mode is the default mode
when you first display a graph. The Y-axis represents
the value of the parameter, the X-axis represents the
order in which the sample runs are stored in
the database.
Some Sequential mode graphs are displayed with

colored bars on the Y-axis representing ranges for that
parameter. Typically:
r Yellow is low
r Green is within range
r Red is high
r White is undefined.
You can select the Zoom mode from the Sequential
mode graph.
Figure 4.2-8 Zoom Mode In the Zoom mode, the graph is split vertically into
two sections.
r The section on the right displays the graph with

a selector bar.
r The section on the left displays a magnified view
showing the five points on either side of the
point selected by the selector bar. To scroll
through the points, use è or é. To accelerate
scrolling, use Ý+è or Ý+é.
r The area of the graph frame below the X-axis
displays the date, time and value of the
selected point.
You can enable the Zoom mode from either the
Sequential or the Date Offset mode.
PN 4235961E 4.2-7
Figure 4.2-9 Histogram Mode In the Histogram mode, the X-axis is divided into
256 channels and the data points are tallied for each
channel.
Figure 4.2-10 Date-Offset Mode In the Date Offset mode the Z-axis changes to reflect
the date and time the sample was run. A line is drawn
to connect the data points.
You can select the Zoom mode from the Date Offset
mode graph to scroll through the data points.
Figure 4.2-11 Control-File Graph On a control file graph, the graph title includes the
parameter displayed, the lot number of the control
and the control file name. The numbers on the right
edge of the graph (1 to 15 or 16 to 30) represent the
different control files available. The number of the
selected file is highlighted.
By default, data points are white unless you enter and

save the assay values for the control. When the assay
values are available, the points on the graph are:
r Yellow if they are below assay limits.

r Green if they are within assay limits.
r Red if they are above assay limits.
Use ç and æ to select other control files.
4.2-8 PN 4235961E
Figure 4.2-12 Service Disk Utilities Menu Tree
SERVICE PARAMETERS ONLY
BACKUP TO FLOPPY COMPLETE DATABASE
FILES GET FLOPPY BACKUP †
SELECT DATA SET †
5C PHASE II ASSAY
RETIC-C ASSAY
LATRON ASSAY
SETUP* LATRON
LATRON OFFSETS
PRINTER PORT
PRINTER SETUP
SERVICE PRINTER TYPE
DISK
GRAPH RANGE
UTILITIES
MENU
ENABLE MAXM SUSPECT FLAGS

UTILITIES COMMUNICATION
HARD DISK INFORMATION
EXIT * The Setup menu contains items for configuring features of the Service Disk
and this configuration is stored on the disk itself. Therefore, do not write
protect this disk.
† Only available when the Service Disk software is installed on a hard

5961291D drive.
PN 4235961E 4.2-9
Table 4.2-2 Service Disk Utilities Selections/Explanations

Selection Description Procedures
Files tt Backup to Allows you to save: To Store Service Parameter Only Data on a
Floppy tt Service r Parameters viewable with Service Disk. Floppy Diskette
Parameters Only Refer to Figure 4.2-1. 1. From the Service Disk Utilities menu, select
r All the control files. Files tt Backup to Floppy tt Service
Parameters Only.
r Other selected files.
2. Follow the directions on the screen.
See Appendix B, DMS DATABASE FIELDS, for an
exact list of what is saved.
This option creates a file (DMSDB.CSV) which:
r Can be restored and viewed using the
Service Disk software installed on a hard
drive. (See To Restore the Data and To Select
a Data Set below in this table.)
r Is compatible with any spreadsheet that
reads “Comma Separated Value” or CSV
files.
You can store several Service Parameter Only
databases on a single high-density floppy
diskette.
Files tt Backup to Allows you to save all: To Store a Complete Database on a Floppy
Floppy tt Complete r Database parameters. Diskette
Database 1. From the Service Disk Utilities menu, select
r Control files.
Files tt Backup to Floppy tt Complete
See Appendix B, DMS DATABASE FIELDS, for an
Database.
exact list of what is saved.
2. Follow the directions on the screen.
This option creates three files. The first record of
each file contains the column headers for that
file.
r RDBALL_A.CSV contains sample results.
r RDBALL_B.CSV contains sample flags and
demographics.
r RDBALL_C.CSV contains graphics which
must be reconstructed for display. This
utility is currently not available.
The data in these files:
r Can be restored and viewed using the
Service Disk software installed on a hard
drive. (See To Restore the Data and To Select
a Data Set below in this table.) Use this
software to display the Service Parameters.
r Is compatible with any spreadsheet that
reads “Comma Separated Value” or CSV
files. Use this type of software to view or
graph the other parameters.
4.2-10 PN 4235961E
Table 4.2-2 Service Disk Utilities Selections/Explanations (Continued)
Files tt Get Floppy ATTENTION: Only available using Service Disk To Restore the Data
Backup software installed on a hard drive. 1. From the Service Disk Utilities menu, select
Allows you to restore data that was archived on Files tt Get Floppy Backup.
a floppy diskette using the Service Parameters 2. Follow the directions on the screen. A list of
Only option. the data sets available on the floppy diskette
is displayed.
3. Use çæ until the desired archive is
highlighted, then press Û.
4. If no error appears on the screen, the
procedure is complete; the database and
control files are extracted and are now
stored on the hard drive in a subdirectory of
C:\DMSGR. To view the data, see the next
procedure, To Select a Data Set below.
Files tt Select Data ATTENTION: Only available using Service Disk To Select a Data Set
Set software installed on a hard drive. 1. From the Service Disk Utilities menu, select
Allows you to select a data set stored on the Files tt Select Data Set. A list of the data
hard drive. When you first start the hard drive sets stored on the hard drive is displayed.
version of the Service Disk software, the 2. Use çæ until the desired data set is
message No Data Set Selected appears. highlighted, then press Û. A list of
parameters available for viewing is
displayed.
3. To select a new data set, press È and then
repeat steps 1 and 2.
To delete a data set, use çæ until the data set
is highlighted, then press á.
Setup tt 5C Phase Allows you to enter and save control file To Add a New Lot Number - 5C Cell Contol
II Assay information for 5C cell control parameters. 1. From the Service Disk Utilities menu, select
When you graph one of these parameters for a
Setup tt 5C Phase II Assay.
particular lot number:
2. Press à.
r If you have entered the assay values for that
3. Type in the values and press Û.
lot number, colored limits are superimposed
on the graph. The points on the graph are - 4. To change the expected range, press Ù to
select that field.
t Green when results are within limits.
t Red when results are above limits. ATTENTION: Check your entries carefully before
t Yellow when results are below limits. saving them. Once a lot number is saved you
cannot edit it.
r If you have not entered the assay values for
that lot number, all the points on the graph 5. Press Ò to save the entries.
are white. To delete a lot number, use çæ until the lot
number is highlighted, then press á.
PN 4235961E 4.2-11

Setup tt Retic-C Allows you to enter and save control file To Add a New Lot Number - Retic C
Assay information for Retic-C control parameters. 1. From the Service Disk Utilities menu, select
When you graph one of these parameters for a
Setup tt Retic-C Assay.
particular lot number:
2. Press à.
r If you have entered the assay values for that
lot number, colored limits are superimposed 3. Type in the values and press Û.
on the graph. The points on the graph are: 4. To change the expected range, press Ù to
t Green when results are within limits. select that field.
t Red when results are above limits. ATTENTION: Check your entries carefully before
t Yellow when results are below limits. saving them. Once a lot number is saved you
cannot edit it.
r If you have not entered the assay values for
that lot number, all the points on the graph 5. Press Ò to save the entries.
are white. To delete a lot number, use çæ until the lot
number is highlighted, then press á.
Setup tt Latron tt Allows you to enter and save LATRON control To Enter the LATRON Assay Values
Latron Assay assay values and limits. 1. From the Service Disk Utilities menu, select
When graphing a LATRON control file, colored Setup tt Latron tt Latron Assay.
limits are superimposed on the graph. The 2. Type in the values and press Û.
points on the graph are:
3. To change the expected range, press Ù to
r Green when results are within limits. select that field.
r Red when results are above limits. 4. Press È to save the entries and return to
r Yellow when results are below limits. the Main Screen menu.
Setup tt Latron tt Allows you to enter the intrument’s mean To Set the LATRON Offsets
Latron Offsets recovered values for LATRON control so that the 1. From the Service Disk Utilities menu, select
instrument can generate limits for the LATRON
Setup tt Latron tt Latron Offsets.
control that are adjusted for the instrument’s
gain setting. 2. Enter the mean recovered values for
LATRON control.
Note: Since the LATRON control parameters are
directly affected by gain, the limits must be 3. Press È to return to the Main Screen
adjusted whenever the LATRON control mean menu.
results are not nominal.
The new, gain-adjusted limits are highlighted on
the graph; the original limits are dimmed.
Setup tt Printer Allows you to select the Printer port that is used To Change the Printer Port
Setup tt Printer when you press Õ to print the display. The 1. From the Service Disk Utilities menu, select
Port available options are LPT1 and LPT2. LPT1 is
Setup tt Printer Setup tt Printer Port.
the default setting.
2. Press ß to switch to the alternate
option.
3. Press È to return to the Main Screen
menu.
4.2-12 PN 4235961E
Setup tt Printer Allows you to select the Printer type that is used To Change the Printer Type
Setup tt Printer when you press Õ to print the display. The 1. From the Service Disk Utilities menu, select
Type available options are Epson (typically dot matrix)
Setup tt Printer Setup tt Printer Type.
and HP3 (Hewlett Packard Laser Jet III). Epson
is the default setting. 2. Press ß to switch to the alternate
option.
menu.
Setup tt Graph Allows you to limit the number of points To Change the Number of Days of Data Included
Range graphed to a set number of days from the date in the Graphs
of the last record in the database. The default 1. From the Service Disk Utilities menu, select
setting is 120 days. Setup tt Graph Range.
Note: This option affects only the graphing of 2. Type in the number of days of data you want
data points. It does not affect the capturing of included in the graphs and press Û.
data using the Backup to Floppy option.
menu.
Utilities tt Enable Enables or disables the MAXM analyzer Suspect To Enable or Disable the Suspect Flags
MAXM Suspect Flags. Menu selection displayed varies 1. From the Service Disk Utilities menu, select
Flags according to the disposition of the Suspect
Utilities.
Flags.
2. If Suspect Flags are disabled, select Enable
r If the Suspect Flags are disabled, the menu MAXM Suspect Flags to enable them.
selection is Enable MAXM Suspect Flags.
3. If Suspect Flags are enabled, select Disable
r If the Suspect Flags are enabled, the menu MAXM Suspect Flags to disable them.
selection is Disable MAXM Suspect Flags.
Utilities tt Selecting this menu item starts the SIMPCOM 1. Connect the appropriate communications
Communication communications program. When the program cable to the Bar-Code Reader Decoder card.
first loads, the default settings are the settings Refer to Connecting the Bar-Code
required for communication to the Bar-Code Communications Cable under Heading 4.38,
Reader Decoder card. BAR-CODE READER DECODER CARD
CONFIGURATION.
2. From the Service Disk Utilities menu, select
Utilities tt Communication.
3. Type < D > to display the Microscan Main
Menu.
4. Reconfigure the Bar-Code Reader
Decoder card. Refer to Selecting the Settings
for the Microscan™ Bar-Code Reader
Decoder Card under Heading 4.38,
BAR-CODE READER DECODER CARD
CONFIGURATION.
PN 4235961E 4.2-13

Utilities tt Hard On computers with an IDE-type hard drive,
Disk Information displays information about the hard drive, such
as type, serial number and formatted capacity.
Note: If the computer has an MFM drive, such
as the one used on the Intel 301Z, it generates
an error.
4.2-14 PN 4235961E
WHOLE BLOOD VERIFICATION - CBC/DIFF 4
4.3 WHOLE BLOOD VERIFICATION - CBC/DIFF
Purpose
Use this procedure to determine the CBC and diff instrument performance.
Note: This procedure allows you to check the instrument’s reproducibility, plus the
differential counts and the scatterplots for the specimen. The reproducibility procedure
(Heading 4.5, REPRODUCIBILITY CHECKS) only checks the instrument’s reproducibility.
B Service Disk
B 5 mL of normal whole blood drawn in K3EDTA within the last 5 hours
Procedure
1. Activate the service options as directed under Heading 4.2, USING THE SERVICE DISK.
2. Ensure that Auto print is turned on via Í Optns.
3. Ensure the Diff mode is enabled via Ë Run, Î DIFF ON/OFF.
4. Select the Calibration Reproducibility screen (Special Functions tt Calibration tt
Reproducibility). Delete the file before running this test.
ATTENTION: Fresh whole blood must be used for all parameters. Run this test carefully and
rapidly to minimize excessive data drifts. If the test is interrupted, it will cause a five-part diff
result to appear as though there were a system failure, when, in fact, there was not a failure.
5. Aspirate 11 samples from the whole blood specimen, and delete the first one.
6. From the Reproducibility screen, print a matrix copy of the file. Ensure that the data in
the file falls within the limits specified in Table A.1-10, CBC and Diff Reproducibility
Limits.
7. Ensure the scatterplots appear similar and do not have obvious differences.
8. Examine each scatterplot for good separation. Good separation means:
r In DF1 the LY and MO populations are separated from the NE population, the NE
population is centered in its region, and the LY population is fairly rounded and
well-defined.
r The LS histogram has a deep valley.
Note: If the optical axis is not focused properly, separation is lost, the LY population is
distorted, and the LS histogram has a shallower valley. The LATRON control is not
sensitive to optical defocusing; it could still have a very good CV, narrow histogram and
small gain reduction.
PN 4235961E 4.3-1
WHOLE BLOOD VERIFICATION - CBC/DIFF
ATTENTION: Do not attempt to correct optical focusing by adjusting the LS gain. Realign the
optical axis. Always use normal blood for verification of the optical adjustment.
9. If the separation of the five-part diff populations is poor, try another specimen to rule out
the possibility of a "bad" specimen. If the separation on the second specimen is also
poor, this is probably an instrument problem. Refer to Heading 4.9, VCS
OPTIMIZATION.
Note: If the CV% for V, C, and S are passing specification but still produce poor
separation, investigate and take corrective action.
10. Ensure the cell count is >7,750 for at least eight of the blood samples. Two samples may
fall below 7,750 as long as they are >7,000.
11. Ensure that the count time is within the proper time limits for the sample used.
12. If the instrument fails the criteria by a small increment, then repeat steps 5 and 6. If the
instrument fails again, review the data for possible failures and take corrective action.
4.3-2 PN 4235961E
LATEX CALIBRATION AND VERIFICATION 4
4.4 LATEX CALIBRATION AND VERIFICATION
Purpose
This section includes two latex calibration and verification procedures.
r Use the CBC Latex Calibration and Verification procedure to verify the aperture voltages
are adjusted correctly.
r Use the Diff and Retic Latex Calibration and Verification procedure to verify the DC, RF,
and LS gains are adjusted correctly.
CBC Latex Calibration and Verification
B Service Disk
CAUTION Deteriorated latex particles can clog the apertures. Latex particles deteriorate when exposed to
direct sunlight or to temperatures above 45°C (113°F). DO NOT use latex particles that have been exposed
to these conditions.
IMPORTANT Making adjustments with insufficient data could produce erroneous results. The particle
concentration of LATRON control is low. DO NOT use LATRON control for this procedure.
B 94.3 fL latex particles, PN 6857371
Procedure
2. From the Service Options menu, select Latex Checks tt Red & White Channel Calibration.
3. Press Ê to enter the assay value for the latex particles. Use the numeric keypad to enter
the assay value indicated on the vial, then press Û.
4. Press Ë to prepare the unit for latex sampling. The System Status field displays
R/W LTX CAL ON when ready.
5. Immerse the aspirator tip in a well-mixed vial of the latex particles, and press the
sample bar.
6. Verify that the counts on the DMS screen are within the limits specified in Table A.1-3,
CBC Latex Calibration.
7. Verify that the RBC Mode and WBC Mode values are within the limits specified in
Table A.1-3, CBC Latex Calibration.
8. Verify that the RBC and WBC aperture current voltages are within the limits specified in
Table A.1-3, CBC Latex Calibration.
9. If the modes are unacceptable:
a. Adjust either the RBC or WBC aperture current voltage at the R/W Preamp card
using the computed voltage displayed on the DMS, ensuring that they are within the
limits specified in Table A.1-3, CBC Latex Calibration.
b. Repeat from step 5.
PN 4235961E 4.4-1
LATEX CALIBRATION AND VERIFICATION
10. If the modes and aperture current voltages are acceptable and you did this procedure as
part of installation, print out the results and attach the printouts to the installation test
data log sheets as directed in Verifying the CBC Latex Calibration, step 2, under
Heading 3.7, ADJUSTMENTS AND CALIBRATION.
11. Press Ò to stop the CBC-Latex Calibration routine.
Diff and Retic Latex Calibration and Verification
B Service Disk
B LATRON primer, PN 7546915
B LATRON control, PN 7546914
B LATEX PARTICLES DC/RF, PN 6605419 (for DC and RF gain adjustments only)
Procedure
1. Ensure that the system and the laser have been on for at least 15 minutes.
3. From the Service Options menu, select Latex Checks tt Diff Latex Calibration.
4. Press Ë Secondary. The System Status field displays DIFF LTX CAL ON when ready.
5. Immerse the aspirator tip in a vial of LATRON primer, and press the sample bar.
Maintain the aspirator tip in the vial until a beep sounds at the DMS.
6. When the DMS displays the message, To stop rerun, press aspiration switch now, press the
sample bar to quit the continuous counts function.
7. Verify that the count is within the limits specified for service in Table A.1-17,
LATRON™ Primer Background Limits. Repeat if necessary.
8. Prepare the LATEX PARTICLES DC/RF as directed in the Product Insert for the LATEX
PARTICLES DC/RF.
IMPORTANT Doing continuous counts on the large LATEX PARTICLES DC/RF allows those particles to
collect in the flow cell, which shifts the RF gain and can plug the flow cell. To gather LATEX PARTICLES
DC/RF data, always do single counts on samples of the prepared LATEX PARTICLES DC/RF suspension.
9. Cycle a sample of the LATEX PARTICLES DC/RF suspension. When the DMS displays
the message, To stop rerun, press aspiration switch now, press the sample bar to quit the
continuous counts function.
10. Ensure the DC and RF results are within the limits specified on the Product Insert for the
LATEX PARTICLES DC/RF.
11. If the DC and RF results not within limits, adjust the gains for DC (R17) and RF (R26)
on the RF Detector Preamp card until the mean channels for DC and RF are the assigned
assay values on the Product Insert for the LATEX PARTICLES DC/RF.
12. Repeat steps 9 through 11 until the DC and RF results are within limits.
4.4-2 PN 4235961E
LATEX CALIBRATION AND VERIFICATION 4
13. Cycle five samples of the LATEX PARTICLES DC/RF suspension, doing single counts on
each, and ensure the average of the DC and RF results are within the limits specified on
the Product Insert for the LATEX PARTICLES DC/RF.
14. If the DC and RF results not within limits, correct the problem and repeat this procedure.
Go to Heading 7.4, DC, RF, OR LS LATEX CALIBRATION PROBLEMS.
15. Adjust the LS gain in the Diff mode.
a. Cycle one sample of LATRON primer:
1) Immerse the aspirator tip in a vial of LATRON primer, and press the sample
bar. Maintain the aspirator tip in the vial until a beep sounds at the DMS.
2) When the DMS displays the message, To stop rerun, press aspiration switch now,
press the sample bar to quit the continuous counts function.
Note: It is good practice to flush the system with LATRON primer whenever you
change the particle types.
b. Verify that the count is within the limits specified for service in Table A.1-17,
LATRON™ Primer Background Limits.
c. Cycle one sample of LATRON control and ensure that the count is 8192 and that the
diff LS mean result is within the limits specified in Table A.1-18, LATRON™
Control Calibration and Verification Limits.
d. If the LS mean criterion is not met, adjust the LS gain as needed. Use R8 (the left
potentiometer) on the LS Preamp 5 module to adjust the LS gain in the Diff mode.
e. Repeat steps c and d until the diff LS mean is within limits.
16. Verify the DC, RF, and LS gains in the Diff mode.
a. Cycle five samples of LATRON control, doing single counts on each, and print out
the results of each count.
b. Ensure the mean channels, CVs, and mean to mode differences for each set of
results, and the average of the diff LS mean results are within the limits specified in
Table A.1-18, LATRON™ Control Calibration and Verification Limits.
c. If the DC, RF, or LS results not within limits, correct the problem and repeat this
procedure. Go to Heading 7.4, DC, RF, OR LS LATEX CALIBRATION PROBLEMS.
d. If you checked the gains in the Diff mode as part of installation, attach printouts of
the five sets of results to the Installation Test Data Log Sheets as directed in Verifying
the Diff and Retic Latex Calibration, step 2, under Heading 3.7, ADJUSTMENTS
AND CALIBRATION.
17. If the MAXM hematology analyzer is a Retic analyzer, adjust the LS gain in the
Retic mode.
a. From the Service Options menu, select Latex Checks tt Retic Latex Calibration.
b. Press Ë Secondary. The System Status field displays RET LTX CAL ON when ready.
c. Cycle one sample of LATRON control in the Retic mode and verify that the count is
8192 and that the retic LS mean result is within the limits specified in Table A.1-18,
LATRON™ Control Calibration and Verification Limits.
d. If the LS mean criterion is not met, adjust the LS gain as needed. Use R16 (the right
potentiometer) on the LS Preamp 5 module to adjust the LS gain in the Retic mode.
e. Repeat steps c and d until the retic LS mean result is within limits.
PN 4235961E 4.4-3
LATEX CALIBRATION AND VERIFICATION
18. If the MAXM hematology analyzer is a Retic analyzer, verify the DC, RF, and LS gains in
the Retic mode.
a. Cycle five samples of LATRON control, doing single counts on each, and print out
b. Ensure the mean channels, CVs, and mean to mode differences for each set of
results, and the average of the retic LS mean results are within the limits specified in
Table A.1-18, LATRON™ Control Calibration and Verification Limits.
c. If the DC, RF, or LS results are not within limits, correct the problem and repeat this
procedure. Go to Heading 7.4, DC, RF, OR LS LATEX CALIBRATION PROBLEMS.
d. If you checked the gains in the Retic mode as part of installation, attach printouts of
the five sets of results to the Installation Test Data Log Sheets as directed in Verifying
the Diff and Retic Latex Calibration, step 3, under Heading 3.7, ADJUSTMENTS
AND CALIBRATION.
19. If you checked or adjusted the LS gains as part of the VCS Optimization procedure,
continue with that procedure. Measure the noise levels as directed in Diff and Retic RMS
Noise Checks under Heading 4.12, RMS NOISE CHECKS.
4.4-4 PN 4235961E
REPRODUCIBILITY CHECKS 4
4.5 REPRODUCIBILITY CHECKS
Purpose
Use the procedures in this section to check the reproducibility of the instrument in the
Primary mode, Secondary mode, and Reticulocyte mode (if applicable). For more detailed
procedures, refer to the Special Procedures and Troubleshooting manual.
Verifying Reproducibility in the Primary Mode
B A minimum of two tubes of normal, whole-blood with parameter values within the limits
specified in Tables A.1-8 and A.1-9.
Note: If you are doing this procedure as part of Secondary mode - to - Primary mode
calibration, you may need additional blood from the same donor.
Procedure
1. Select Reproducibility (Special Functions tt Calibration tt Reproducibility).
2. If running a MAXM analyzer with Rotary Cap-Pierce module:
a. Press Ê Start Primary to initiate a Primary-mode Reproducibility cycle. The System
Status displays REPRO 1° ON when ready.
r If the DMS displays MODE REQUIRES EXISTING RUNS TO BE DELETED. ARE
YOU SURE/: NO, press ßÛ to delete the reproducibility table.
r If the reproducibility table contains data, press ÐßÛ to delete
the data.
b. Cycle a sample from one of the specimen tubes as the prime sample.
c. Cycle the other specimen tube 10 times.
3. If running a MAXM analyzer with Autoloader module:
a. Set the number of aspirations per tube to one.
b. Press Ê Start Primary to initiate a Primary-mode Reproducibility cycle. The System
Status displays REPRO 1° ON when ready.
r If the DMS displays MODE REQUIRES EXISTING RUNS TO BE DELETED. ARE
YOU SURE/: NO, press ßÛ to delete the reproducibility table.
r If the reproducibility table contains data, press ÐßÛ to delete the
data.
c. Place the cassette in the loading bay and run the prime sample.
d. When the instrument completes the prime cycle, press Ë Run Ñ Stop and then
reset the number of aspirations per tube to 10.
e. Press Ê Start Primary to initiate a Primary-mode Reproducibility cycle.
f. Insert the remaining tube into an empty cassette and place the cassette in the
loading bay. The instrument automatically processes the specimen 10 times.
4. Observe the instrument while it is cycling and ensure proper operation.
PN 4235961E 4.5-1
REPRODUCIBILITY CHECKS
5. When the cycles are completed, compare the results to the limits specified in
Table A.1-10, CBC and Diff Reproducibility Limits.
Note: The results of the prime run are automatically deleted from the calculations.
6. If any CV or maximum range is out of tolerance, the reproducibility has failed. Correct
the problem and repeat the test.
7. If the results are within the tolerances and if you did this procedure as a preliminary step
in mode-to-mode calibration and/or as part of installation, save the remainder of the
normal whole-blood specimen and a copy of the reproducibility table.
8. If you did this procedure as part of installation, print out and attach the completed
reproducibility table to the Installation Test Data Log Sheets as directed in Checking
Reproducibility and Carryover in the Primary Mode, step 2, under Heading 3.7,
ADJUSTMENTS AND CALIBRATION.
9. Press ËÑ to return the System Status field to Select Function, and select the desired
mode.
10. Press ÈÑ to access the Main menu.
Verifying Reproducibility in the Secondary Mode
B One normal, whole-blood specimen with parameter values within the limits specified in
Tables A.1-8 and A.1-9.
Procedure
1. Select Reproducibility (Special Functions tt Calibration tt Reproducibility).
2. If the System Status field is not displaying Select Function, press Ñ Stop to change the
System Status to Select Function.
3. Press Ë Secondary. The System Status field displays REPRO 2° ON when ready.
r If the DMS displays MODE REQUIRES EXISTING RUNS TO BE DELETED. ARE YOU
SURE/: NO, press ßÛ to delete the reproducibility table.
r If the reproducibility table contains data, press ÐßÛ to delete the data.
4. Run eleven samples of the specimen in the Secondary mode.
Note: The first of the eleven samples is the prime sample.
a. Mix and uncap the fresh, normal whole-blood specimen.
b. Immerse the aspirator tip in the specimen and press the start bar.
c. Repeat steps a and b for a total of 11 times.
5. Compare the results to the limits specified in Table A.1-10, CBC and Diff Reproducibility
Limits.
6. If any CV or maximum range is out of tolerance, the reproducibility has failed. Correct
the problem and repeat the test.
7. If the results are within the tolerances and if you did this procedure as part of
installation, print out and attach the completed reproducibility table to the Installation
Test Data Log Sheets as directed in Checking Reproducibility and Carryover in the
Secondary Mode, step 2, under Heading 3.7, ADJUSTMENTS AND CALIBRATION.
4.5-2 PN 4235961E
REPRODUCIBILITY CHECKS 4
mode.
Verifying Retic % Reproducibility (Sample Analysis)
B One tube of normal, whole-blood with sufficient quantity to pipet a minimum of 500 µL
B Reagent A
B Reagent B with assembled Statmatic dispenser
B Two air displacement pipets fixed or adjustable, one calibrated to 50 µL and the other
to 2 µL
B Test tubes
Procedure
1. Prepare the whole-blood specimen with the ReticPrep™ reagents using the procedure on
the package insert of the ReticPrep reagent kit.
2. After 5 minutes incubation, and one at a time, prepare 10 clearing solution samples
(Reagent B) from the one stained sample, analyzing each after 30 seconds as described in
the ReticPrep procedure.
3. From the DMS Main menu, select Special Functions tt Calibration tt Reproducibility.
4. From the Run screen, press Ì Retic.
5. Cycle the samples prepared in step 2, 11 consecutive times, deleting the first result.
6. Verify that the CV % passes. See Table A.1-11, Retics Reproducibility Limits.
7. If you checked Retic % reproducibility as part of installation, go back to Checking
Reproducibility and Carryover for the Retic Parameter under Heading 3.7,
ADJUSTMENTS AND CALIBRATION, and begin at step 3.
PN 4235961E 4.5-3
REPRODUCIBILITY CHECKS
4.5-4 PN 4235961E
CARRYOVER CHECKS 4
4.6 CARRYOVER CHECKS
Purpose
Use the procedures in this section to check the carryover of the instrument in the Primary
mode, Secondary mode, and Reticulocyte mode (if applicable).
Checking Carryover in the Primary Mode
B Service Disk
B Two normal, whole-blood specimens (only one is needed on instruments with a Rotary
Cap-Pierce module)
B Three specimen tubes with clean diluent
Procedure
2. From the Service Options menu, select Certification tt Carryover.
4. If the carryover table contains data, delete the data.
5. Press Ê Start Primary. The System Status field displays CARRYOVER 1° ON when ready.
6. If running a MAXM with Autoloader module:
a. Insert the two normal, whole-blood specimen tubes into positions 1 and 2 of a
cassette, and insert the three tubes of clean diluent into positions 3 through 5.
b. Place the cassette in the loading bay to initiate the cycle.
7. If running a MAXM with Rotary Cap-Pierce module:
a. Cycle the tube of normal whole-blood twice.
b. Cycle the three tubes of clean diluent.
8. Observe the instrument while cycling and ensure the cycle finishes correctly.
9. Ensure the carryover results are within the limits specified in Heading A.1-6,
Primary/Secondary-Mode Carryover Limits.
10. If the results are out of tolerance, the carryover has failed. Correct the problem and
repeat this test.
11. If the results are within tolerance and you did this procedure as part of installation, print
out and attach the completed carryover table to the Installation Test Data Log Sheets as
directed in Checking Reproducibility and Carryover in the Primary Mode, step 4, under
mode.
PN 4235961E 4.6-1
CARRYOVER CHECKS
Checking Carryover in the Secondary Mode
B Service Disk
B One normal, whole-blood specimen
B Diluent in a clean vial or specimen tube
Procedure
2. From the Service Options menu, select Certification tt Carryover.
4. If the carryover table contains data, delete the data.
5. Press Ë to select the Secondary mode. The System Status field displays
CARRYOVER 2° ON when ready.
6. Cycle two samples from the specimen tube in the Secondary mode, then cycle diluent
three times to obtain the carryover results.
7. Ensure that the carryover results are within limits specified in Heading A.1-6,
Primary/Secondary-Mode Carryover Limits.
8. If the results are out of tolerance, the carryover has failed. Correct the problem and
repeat this test.
9. If the results are within the tolerances and if you did this procedure as part of
installation, print out and attach the carryover table to the Installation Test Data Log
Sheets as directed in Checking Reproducibility and Carryover in the Secondary Mode,
step 4, under Heading 3.7, ADJUSTMENTS AND CALIBRATION.
mode.
Retic Carryover Tests
B Normal whole blood, sufficient quantity to pipet and aspirate a minimum of 1 mL
B Reagent A
B Reagent B with assembled Statmatic dispenser
B One air displacement pipet, fixed or adjustable, calibrated to 50 µL
B One air displacement pipet, fixed or adjustable, calibrated to 2 µL
B Test tubes
B Service Disk
4.6-2 PN 4235961E
CARRYOVER CHECKS 4
IMPORTANT Performing the Retic carryover test procedures out of order can affect the results. Always
perform the Retic carryover test procedures in this order:
1. General Instructions
2. Mode-to-Mode
3. Within Mode.
General Instructions
1. Ensure that background counts are within limits before performing this test.
3. Ensure that the parallel printer is on-line and auto print is enabled.
Retic Mode-to-Mode Carryover

1. From the Main menu, select Sample Analysis tt Run Samples.
2. At the Run screen:
a. Ensure that Diff is enabled. If it is not, press Î Diff On/Off.
b. Press Ê Start Primary.
3. Cycle three samples of whole blood in the Primary mode. Ensure that the cell count for
the Diff is within the limits specified in the footnote in Table A.1-7, Retic Mode-to-Mode
and Within-Mode Carryover Limits*.
4. From the Run screen, press Í Retic.
5. Dispense 2 mL of Reagent B into six clean test tubes.
Note: Set three of these tubes aside for the Retic Within Mode procedure below.
6. Aspirate three Reagent B samples (rinses) in the Retic mode and print out the results of
each count.
7. Verify that the results are within the tolerances specified for mode-to-mode carryover in
Table A.1-7, Retic Mode-to-Mode and Within-Mode Carryover Limits*.
r If the results are out of tolerance, the carryover has failed. Correct the problem and
repeat this test.
r If the results are within the tolerances and if you did this procedure as part of
installation, attach the printouts to the Installation Test Data Log Sheets as directed
in Checking Reproducibility and Carryover for the Retic Parameter, step 4, under
8. Check the Retic within-mode carryover. See Retic Within Mode below.
Retic Within Mode

1. Prepare whole blood specimen with ReticPrep™ reagents using procedure on package
insert of ReticPrep reagent kit.
a. Allow stained specimen to incubate 5 minutes.
b. One at a time, prepare three clearing solution samples (Reagent B) from the one
stained sample.
2. Aspirate three Retic samples followed by three Reagent B samples (rinses) and print out
PN 4235961E 4.6-3
CARRYOVER CHECKS
3. Verify that the results are within the tolerances specified for within mode carryover in
Table A.1-7, Retic Mode-to-Mode and Within-Mode Carryover Limits*.
r If the results are out of tolerance, the carryover has failed. Correct the problem and
repeat this test.
r If the results are within the tolerances and if you did this procedure as part of
installation, attach the printouts to the Installation Test Data Log Sheets as directed
in Checking Reproducibility and Carryover for the Retic Parameter, step 6, under
4.6-4 PN 4235961E
INITIAL ADJUSTMENT OF PRIMARY-MODE CALIBRATION FACTORS 4
4.7 INITIAL ADJUSTMENT OF PRIMARY-MODE CALIBRATION FACTORS
Purpose
You adjust the Primary-mode calibration factors to ensure accuracy of the instrument’s
results. Use this procedure to make the initial adjustment of the Primary-mode calibration
factors. The laboratory is responsible for the final adjustment of the calibration factors using
S-CAL calibrator or a whole blood calibration procedure.
B 5C Normal cell control
Procedures
1. Select Special Functions tt Calibration tt CBC Calibration.
2. If running a MAXM analyzer with Autoloader module, set the number of aspirations per
tube to 11.
Note: The maximum number that can be entered is 12.
3. Press Ê Start Primary to select the Primary-mode cycle. The System Status field displays
CALIBRATION ON when ready.
4. Obtain the assay sheet for 5C Normal cell control, and enter the MAXM analyzer assay
values for WBC, RBC, Hgb, MCV, Plt, and MPV into the Ref. Values field on the CBC
Calibration screen.
5. Press Ê Run/Ref to place the cursor on the run line for sample number one.
6. Allow the controls to come to room temperature and then mix according to control
package insert.
7. Cycle the 5C Normal cell control 11 times. If running a MAXM analyzer with Autoloader
module, place the tube of cell control into a 5-mL tube-size cassette and place the
cassette into the loading bay. The system automatically processes the specimen 11 times
based on what was entered in step 2.
8. Observe the instrument and ensure proper operation of the system while cycling.
9. When the instrument completes the cycles, review the data:
a. Check results for trending. Take appropriate action if trending is present.
b. Verify results fall within the following maximum CV% for each parameter specified
in Table A.1-2, Acceptable CV Limits for Initial Adjustments to 5C Cell Control.
c. Verify the "New Cal Factors" are within the ranges specified in Table A.1-1,
Acceptable Cal Factor Ranges.
10. Press Ñ Stop to return the System Status to Select Function.
11. Select all parameters for calibration:
a. Press Í Optns.
b. Select Select Parameters.
c. Set YES for all parameters. Use the ß to switch between NO and YES.
d. Press È.
PN 4235961E 4.7-1
INITIAL ADJUSTMENT OF PRIMARY-MODE CALIBRATION FACTORS
12. Select Transmit Factors to transmit the new factors. If the optional Graphic Printer is
installed, when prompted press ß to answer YES and then press Û to print
the data.
Note: If a Graphic Printer is not installed, record the data. When you transmit the new
calibration factors, the calibration batch table is deleted and the OLD CAL Fac field
displays the new, current calibration factors.
13. If you did this procedure as part of installation, attach the printout (or record the data)
on the Installation Test Data Log Sheets as directed in Making Initial Primary-Mode
Calibration Adjustments, step 3, under Heading 3.7, ADJUSTMENTS AND
CALIBRATION.
14. Press È to exit the calibration table and display the Calibration menu.
4.7-2 PN 4235961E
SECONDARY MODE-TO-PRIMARY MODE CALIBRATION 4
4.8 SECONDARY MODE-TO-PRIMARY MODE CALIBRATION
Purpose
Use this procedure to calibrate the Secondary-mode results to ensure they match the
Primary-mode results.
ATTENTION: If the whole-blood specimen and results from the Primary-mode reproducibility
check are not available, do the Verifying Reproducibility in the Primary Mode procedure
under Heading 4.5, REPRODUCIBILITY CHECKS, and save the specimen and results for use
with this procedure.
B Service Disk
B The normal, whole-blood specimen used for the Primary-mode reproducibility check
and the results obtained from that check.
Procedure
2. From the Service Options menu, select Secondary Mode Cal.
3. Press Ë to select the Secondary mode. The System Status field displays
SEC MODE CAL ON when ready.
4. In the reference value fields, enter the mean value for each parameter (WBC, RBC, Hgb,
MCV, Plt and MPV) from the Primary-mode reproducibility check printout. Press Û
after each entry.
5. In the Secondary mode, run eleven samples of the whole-blood specimen used in the
Primary-mode reproducibility check:
a. Mix and uncap the fresh, normal whole-blood specimen.
b. Immerse the aspirator tip in the specimen and press the sample bar.
c. Repeat steps a and b for a total of 11 times.
6. Ensure that the New Cal Factors displayed are within the limits specified for the
Secondary mode in Table A.1-1, Acceptable Cal Factor Ranges.
7. Select the parameters to be calibrated:
a. Press Í Optns.
b. Select Select Parameters.
c. Set YES for parameters that need calibration, NO for the others. Use the ß to
switch between YES and NO.
d. Press È.
8. Select Transmit Factors to transmit the new factors. If the optional Graphic Printer is
installed, when prompted press ß to answer YES and then press Û to print
the data.
Note: If a Graphic Printer is not installed, record the data. When you transmit the new
calibration factors, the calibration batch table is deleted and the OLD CAL Fac field
displays the new, current calibration factors.
PN 4235961E 4.8-1
SECONDARY MODE-TO-PRIMARY MODE CALIBRATION
9. If you did this procedure as part of installation, attach the printout (or record the data)
on the Installation Test Data Log Sheets as directed in Verifying the Secondary Mode - to
- Primary Mode Calibration, step 2, under Heading 3.7, ADJUSTMENTS AND
CALIBRATION.
10. Repeat steps 5 through 6 for five whole blood cycles to verify calibration.
11. Compare the mean values obtained in the Secondary mode to the reference values. They
should be within the limits specified in Table A.1-12, Secondary Mode-to-Primary Mode
Comparison Limits.
12. If you did this procedure as part of installation, print out the verification data and attach
the printout (or record the data) on the Installation Test Data Log Sheets as directed in
Verifying the Secondary Mode - to - Primary Mode Calibration, step 3, under
13. Press ÈÑ to return to the Main menu.
4.8-2 PN 4235961E
VCS OPTIMIZATION 4
4.9 VCS OPTIMIZATION
Purpose
Many factors can affect blood results obtained using VCS technology. You use this procedure
to optimize the instrument-related factors, thereby minimizing the impact of factors beyond
Coulter’s control, such as specimen handling and environmental problems.
Figure 4.9-1 Optimization Procedure Flow Perform this procedure whenever you replace a
VCS technology component, which includes:
Perform preliminary
checkout. r Flow cell
r LS sensor
Check count ratio for r RF Detector Preamp card
general unit state. r RF Power Supply
r RF transformer
r LS Preamp module
Set sample and sheath
pressures using flow rate. r Laser REF 2 card
r Laser Power Supply.
Adjust Clog
The overall VCS Optimization procedure is
Detector circuit. further divided into nine procedures.
Figure 4.9-1 shows the recommended procedure
flow for VCS optimization; Table 4.9-1 gives the
purpose of each procedure and lists the tools and
Adjust DC, RF,
and LS gains.
supplies needed. You should be completely
familiar with the entire VCS Optimization
procedure before you begin.
Measure DC, RF, The procedures within the VCS Optimization

and LS noise. procedure are presented linearly because that is
the simplest way to approach them. Because of
the close interrelationship of many of the
Adjust Erythrolyse II and adjustments however, that approach does not
StabiLyse reagent pumps. always work. For example:
r You cannot successfully align the TTM if the

flow rate is incorrect, or if there is excess
Do noise on one of the channels. Therefore, you
LATRON control,
must correct any grossly misadjusted items
NO 5C II cell control, laser
on current/LS offset voltage, before attempting the overall optimization.
noise checks, flow rate, r After you have completed certain
and count ratio adjustments, you may have to go back and
pass?
redo, or at least fine tune, previous
YES adjustments.
TTM is
optimized
5961274D
PN 4235961E 4.9-1
VCS OPTIMIZATION
See Table 4.9-1.
Table 4.9-1 Purpose of VCS Optimization Procedures
Perform... To Ensure... Tools/Supplies Needed

A. Preliminary Checks No obvious fluidic or electronic
sources of noise.
B. Count Ratio Check Overall performance of instrument is B Service Disk
good.
C. VCS Flow-Rate Sheath and sample pressures are set B LATRON primer, PN 7546915
Adjustment for accurate, precise flow through B LATRON control, PN 7546914
flow cell aperture.
B Service Disk
B Fresh, normal, whole blood (if adjustments are
needed)
D. Clog Detector Circuit Clog Detector circuitry detects clogs B Digital voltmeter
Adjustment and starts an “autoclearing” cycle B Thermometer accurate to ±1°F or ±0.5°C
when it does.
E. RF Detector Preamp Minimum noise on RF channel. B True RMS meter
Card C1 Adjustments B Latex particles DC/RF, PN 6605419
F. DC, RF, and LS Gains DC, RF, and LS gains are set for B Service Disk
Adjustments optimum positioning of WBC B Latex particles DC/RF, PN 6605419
subpopulations on the scatterplot.
G. DC, RF and LS Noise Minimum noise on the DC, RF, and B True RMS meter
Measurements LS channels.
H. Erythrolyse II and Reagent volumes used produce a B ACCUVETTE II® vial or equivalent
StabiLyse Reagent final dilution with conductivity and
B Service Disk
Pumps Volume osmolality needed to achieve best
Adjustments count ratio and cell positioning. B Volumetric Cylinder, PN 5415374
B Normal whole blood specimen mixed for at least
30 minutes before using. See the Specimen
Collection section of the Operator’s Guide for
anticoagulant and storage specifications.
B 5C Normal cell control with 5C II cell control
Assay Sheet
I. Verification Static and dynamic noise in both Diff B LATRON primer, PN 7546915
and Retic modes are within B LATRON control, PN 7546914
acceptable limits.
B 5C Normal cell control with 5C II cell control
Instrument is performing to Assay Sheet
specifications. B Normal whole-blood specimen mixed for at least
30 minutes before using. See Specimen
Collection section of the Operator’s Guide for
anticoagulant and storage specifications.
4.9-2 PN 4235961E
VCS OPTIMIZATION 4
A. Preliminary Checks
1. Run a Startup cycle.
2. Check the TTM area for leaks.
3. Check the sheath lines, flow cell, and Erythrolyse II and StabiLyse reagent pumps and
tubing for trapped bubbles.
4. Ensure that the interconnecting electrical cables within the TTM are properly dressed
and connected.
5. Ensure that the LS amplifier output signal cable, the braided grounding strap, and the
green grounding wire:
r Have smooth radius bends.
r Do not prevent the laser base mount assembly from floating freely.
6. Ensure that the base mounts are intact and that the TTM assembly "floats" freely.
B. Count Ratio Check

1. Access the Service Disk software as directed in Using the Service Disk Software on a DMS
under Heading 4.2, USING THE SERVICE DISK.
2. Display the count ratio data in the Histogram mode.
Note: The Histogram mode gives a better view of the data than the Sequential mode.
3. Determine if the count ratio for the instrument is acceptable.
Note: On instruments that are properly optimized and that analyze primarily normal
samples, the count ratio should be >7750 on >90% of the samples. See Figure 4.9-2 and
Figure 4.9-3 for an example of a good and a poor count ratio.
Figure 4.9-2 Histogram of Good Count Ratio Data Figure 4.9-3 Histogram of Poor Count Ratio Data
4. If the count ratio is not acceptable, resolve the problem before continuing. Any of the
following can adversely affect the count ratio:
r Incorrect gain settings
r Electronic noise from any of the VCS components
r Reagent conductivity noise due to sheath/sample imbalance (pump volumes)
r Lysing noise from improper RBC lysing
PN 4235961E 4.9-3
VCS OPTIMIZATION
r Incorrect flow rate and/or flow noise from partial plugs or bubbles
r Mechanical failures
r High incidence of abnormal specimens
r Specimen mishandling
r Extreme temperatures.
Note: If the count ratio is still poor after you have performed this optimization
procedure, the most likely cause is sample handling/preparation problems.
C. VCS Flow-Rate Adjustment

Set the sample and sheath pressures as directed under Heading 4.10, VCS FLOW-RATE
ADJUSTMENT.
D. Clog Detector Circuit Adjustment

Adjust the Clog Detector circuit as directed in Clog Detector Circuit Adjustment under
Heading 4.11, RF DETECTOR PREAMP CARD ADJUSTMENTS.
E. RF Detector Preamp Card C1 Adjustments

Adjust C1 on the RF Detector Preamp card as directed in C1 Adjustment under Heading 4.11,
RF DETECTOR PREAMP CARD ADJUSTMENTS.
F. DC, RF, and LS Gains Adjustments

Adjust the DC, RF, and LS gains as directed in Diff and Retic Latex Calibration and
Verification under Heading 4.4, LATEX CALIBRATION AND VERIFICATION.
G. DC, RF and LS Noise Measurements

Verify the DC, RF, and LS noise levels are minimal as directed in Diff and Retic RMS Noise
Checks under Heading 4.12, RMS NOISE CHECKS.
H. Erythrolyse II and StabiLyse Reagent Pumps Volume Adjustments

Adjust the Erythrolyse II reagent and StabiLyse reagent pumps as directed in Erythrolyse II
and StabiLyse Reagent Pumps Adjustment under Heading 4.16, ERYTHROLYSE™ II AND
STABILYSE™ REAGENT PUMPS ADJUSTMENT AND REPLACEMENT.
I. Verification
1. Verify the static noise and the dynamic noise in the Diff mode, and Retic mode if
applicable, are within acceptable limits as directed in Diff and Retic RMS Noise Checks
under Heading 4.12, RMS NOISE CHECKS.
2. Run 5C Normal cell control and ensure that 5C II cell control parameters are within
limits.
3. From the Main menu of the DMS, select Special Functions tt Calibration tt Reproducibility.
Empty the file.
4.9-4 PN 4235961E
VCS OPTIMIZATION 4
4. Set the oscilloscope:
Input Ch. 1
Impedance 1M DC
Vertical 200 mV/div.
Horizontal 0.5 mS/div.
Synch Auto - Ch. 1
5. Connect the scope to the DC channel on the VCS PROCESSOR card.

6. Run one whole blood prime sample.
7. Run five samples of a fresh, well mixed whole-blood specimen.
8. During count, look at the DC channel’s conductivity (baseline) and ensure that the
conductivity:
r Does not exceed 200 mV peak-to-peak.
r Does not change from run-to-run.
Note: The mixing chamber, Erythrolyse II reagent pumps, and StabiLyse reagent pump
are possible causes of these failures.
9. Check the scatterplots for good separation in each run. That is:
r In DF1 the LY and MO populations are separated from the NE population, the NE
population is centered in its region and the LY population is fairly well rounded and
well defined.
r The LS histogram has a deep valley.
10. If you have problems with diff and retic flagging and you did not align the laser and flow
cell as part of VCS optimization, align the laser and flow cell now as directed under
Heading 4.15, LASER/FLOW CELL ALIGNMENT.
11. Verify instrument performance:
CHECKS.
e. Run controls.
PN 4235961E 4.9-5
VCS OPTIMIZATION
4.9-6 PN 4235961E
VCS FLOW-RATE ADJUSTMENT 4
4.10 VCS FLOW-RATE ADJUSTMENT
Purpose
You set the sample and sheath pressures to ensure that sufficient data is accumulated on
abnormally low WBC specimens, which in turn ensures that the diff parameters are accurate
and that the throughput criterion is met.
This procedure uses the Diff: Flow Rate graph of the database to set the sample flow. The Diff:
Flow Rate graph is the product of the WBC count and the five-part diff count time and is a
relatively constant measurement because samples with a high concentration of WBCs are
counted faster than samples with a low concentration.
The flow rate is adversely affected by partial or full plugs in the aperture of the flow cell.
B Service Disk
B Fresh, normal, whole blood specimen (if adjustments are required)
Sheath Pressure Adjustment

2. From the Service Options menu, select Latex Checks tt Diff Latex Calibration.
3. Run one sample of LATRON primer and one sample of LATRON control in the
Diff mode.
4. Note the CV for DC.
5. Adjust the sheath pressure regulator and run LATRON control until you obtain the
minimum CVs for DC.
Tip: Using an oscilloscope, monitor the DC output on the VCS PROCESSOR card during
the cycle for reduction of the amplitude variation (lower CVs).
Sample Pressure Adjustment

1. If the database is empty, run five specimens with a normal WBC (5.0 - 10.0) as patient
samples under Sample Analysis so that they go into the DMS database.
2. Access the current Service Disk’s software options as directed in Using the Service Disk
Software on a DMS under Heading 4.2, USING THE SERVICE DISK.
3. Look at the Diff: Count Ratio and Diff: Flow Rate graphs of the database.
r If the count ratio is good (>90% >7750 when most of the samples are normal) and
the majority of data points on the flow-rate graph are within limits, go to
Verification.
r If the count ratio is poor, correct that problem before setting the flow rate. See
B. Count Ratio Check under Heading 4.9, VCS OPTIMIZATION.
PN 4235961E 4.10-1
VCS FLOW-RATE ADJUSTMENT
r If the majority of the data points on the Diff: Flow Rate graph are not between the
limits, like the example shown in Figure 4.10-1, check the standard deviation (the
spread) of the data points.
Note: Standard deviation refers to the amount of deviation from the mean; the
greater the spread of the data points, the higher the standard deviation.
t If the standard deviation is low, go to step 4.
t If the standard deviation is high, go to step 5.
Figure 4.10-1 Unacceptable Flow-Rate Graph This instrument had flow-rate problems.
The cause of the high standard deviation appears to have
been resolved about two thirds of the way through the data.
The sample pressure still appears to be too high, however,
since the last data points, while tight, are too low.
ATTENTION: A pressure regulator setting is more stable if you adjust the regulator from a low
setting to a high setting. To ensure maximum stability when you need to adjust the pressure
down, over adjust the pressure down and then adjust it back up.
4. If the standard deviation of the data points is low:
a. Adjust the sample pressure.
b. Run several whole blood specimens with a normal WBC count (5.0 - 10.0) as
patient samples under Sample Analysis so that they go into the DMS database.
c. Ensure that sample and sheath pressures do not vary more than 0.02 psi during
COUNT.
d. Check the position of the last data points on the flow-rate graph.
e. Repeat steps a through d until the data points fall within the limits.
f. Verify the instrument is working correctly. Go to Verification.
5. If the standard deviation of the data points is high, check the sample population for
abnormal WBCs.
Note: Abnormal WBC populations can widen the dispersion of data points on the
flow-rate graph.
6. If the sample population has abnormal WBCs and that is the cause of the high standard
deviation:
a. Run several normal samples as patient samples under Sample Analysis so that they
go into the DMS database.
b. Adjust the sample pressure as described under step 4.
4.10-2 PN 4235961E
VCS FLOW-RATE ADJUSTMENT 4
7. If the sample population for WBCs is mostly normal, or if you determine the high
standard deviation is not the result of analyzing abnormal WBC samples, find and
correct the problem.
Troubleshooting Tip: Use a digital pressure meter to determine if the sheath and sample
pressure readings are correct.
8. Verify the instrument is working correctly. Go to Verification.
Verification
1. Verify that the sheath and sample pressures are within the limits specified in
Table A.1-13, Pressure and Vacuum Tolerances.
2. If you did this procedure as part of installation, no further verification is needed at this
time. Print out five patient scatterplots displaying DC count and time and attach the
printouts to the Installation Test Data Log Sheets as directed in Verifying the DC Count
and VCS Flow Rate, step 2, under Heading 3.7, ADJUSTMENTS AND CALIBRATION.
3. If you did this procedure as part of the VCS Optimization procedure, continue with that
procedure. Go to Clog Detector Circuit Adjustment under Heading 4.11, RF DETECTOR
PREAMP CARD ADJUSTMENTS.
CHECKS.
e. Run controls.
PN 4235961E 4.10-3
VCS FLOW-RATE ADJUSTMENT
4.10-4 PN 4235961E
RF DETECTOR PREAMP CARD ADJUSTMENTS 4
4.11 RF DETECTOR PREAMP CARD ADJUSTMENTS
Purpose
Use the following procedures after replacing the RF Detector Preamp card or the RF Detector
Preamp vacuum tube, and as part of VCS optimization.
r Use the Clog Detector Circuit Adjustment procedure to adjust the Clog Detector circuit
for the ambient temperature of the laboratory to compensate for small, temperature
related changes in diluent flow through the flow cell.
r Use the C1 Adjustment procedure to obtain the lowest RMS noise value in the entire
360-degree range of C1.
In addition, check the Clog Detector Circuit Adjustment:
r As part of instrument installation.

r After replacing the -
t Flow cell
t Flow-cell harness
t RF Power Supply.
r Whenever the instrument reports an abnormal number of FC errors (about 5% of all
samples generate an FC error).
Clog Detector Circuit Adjustment
B Digital voltmeter
B Diluent for cycling
B Thermometer accurate to ±1°F or ±0.5°C
Procedure
4. Disable the blood/bubble detector (Main tt Special Functions tt Diagnostics tt Operator
5. Set the digital voltmeter to VOLTS DC and connect it across TP1 and TP4 (ground) on
the RF Detector Preamp card.
6. Measure the voltage at TP1 while cycling diluent in the Primary mode. Record the peak
voltage reading obtained immediately after the RF aperture current is turned ON.
7. With the thermometer, measure and record the ambient room temperature.
PN 4235961E 4.11-1
RF DETECTOR PREAMP CARD ADJUSTMENTS
8. Calculate and record the voltage for TP2 using the appropriate formula below:
Vtp2 = ({[(Taf - 55) x 0.01] + 1} x Vtp1) + 0.225
or
Vtp2 = ({[(Tac - 12.8) x 0.018] + 1} x Vtp1) + 0.225
where:
Vtp1 = Voltage at TP1

Vtp2 = Voltage at TP2
Taf = Ambient temperature Fahrenheit
Tac = Ambient temperature Centigrade
9. Adjust R44 on the RF Detector Preamp card to set the voltage at TP2 to the voltage
calculated in step 8 ±0.05 V.
10. Disconnect the digital voltmeter.
11. Disconnect the RF cable from J67 and run a sample of diluent. Ensure that the clog
detector is triggered and the instrument goes through an "Auto-Clearing" cycle.
12. Reconnect the RF cable.
13. If you did this procedure as part of VCS optimization, go to C1 Adjustment. If not, go to
Verification below.
Verification
1. If you adjusted the Clog Detector circuit as part of installation, no further verification is
needed at this time. Record the results on the Installation Test Data Log Sheets as
directed in Adjusting the Clog Detector Circuit, step 2, under Heading 3.7,
ADJUSTMENTS AND CALIBRATION.
2. Verify instrument performance by checking reproducibility as directed under
C1 Adjustment
B True RMS meter
B Service Disk
B LATEX PARTICLES DC/RF, PN 6605419
Procedure
1. Connect a true RMS meter to the RF (C) channel on the VCS PROCESSOR card.
2. At the Analyzer:
a. Verify that the Diff mode is enabled.
b. Activate the service options as directed in Heading 4.2, USING THE SERVICE
DISK.
4.11-2 PN 4235961E
RF DETECTOR PREAMP CARD ADJUSTMENTS 4
c. From the Service Options menu, select Latex Checks tt Diff Latex Calibration to display
the Diff Calibration screen.
ATTENTION: It may be necessary to aspirate more than one sample of diluent while making
adjustments. If the sample runs out, check for bubbles in the flow cell and perform a purge if
necessary.
3. Cycle a sample of diluent in the Diff-Latex Calibration mode.
ATTENTION: Between count periods the RMS noise will increase. Ensure readings are taken
during the count periods.
4. While the system is in count observe the RMS voltage.
ATTENTION: Use a plastic Trimmer Pot Tool to adjust C1 on the RF Detector Preamp card to
eliminate the interference caused by a metal shaft.
5. Using a plastic Trimmer Pot Tool, make slight adjustments to C1, moving through the
entire 360 degree range. After each adjustment allow the voltage to settle. This will take
time and a lot of patience. Note the lowest reading.
6. Adjust C1 to the lowest RMS value noted in step 5.
7. Verify the gain adjustment is correct. Go to Verification below.
Verification
1. If you did this procedure as part of VCS optimization, continue with that procedure. Go
to Diff and Retic Latex Calibration and Verification under Heading 4.4, LATEX
CALIBRATION AND VERIFICATION.
2. To verify that the RF channel gain is correct, prepare and cycle the LATEX PARTICLES
DC/RF as directed in the product insert for the latex particles, and ensure the results
meet the specifications listed on the product insert.
PN 4235961E 4.11-3
RF DETECTOR PREAMP CARD ADJUSTMENTS
4.11-4 PN 4235961E
RMS NOISE CHECKS 4
4.12 RMS NOISE CHECKS
Purpose
This section includes two procedures for checking noise.
r Use the CBC RMS Noise Checks procedure to ensure the noise on the RBC, WBC, and
Plt channels is within specifications.
r Use the Diff and Retic RMS Noise Checks procedure to ensure the static noise and
dynamic noise on the DC, RF, and LS channels are within specifications.
CBC RMS Noise Checks
B True RMS meter
Procedure
1. Check the instrument and ensure:
r The instrument is ON but not performing any cycles.
r The RBC and WBC baths are filled.
r The apertures are primed.
2. Close all the instrument doors and panels to prevent outside noise interference.
3. Using a true RMS meter, read the test points on the R/W/P PROC card.
a. Open the upper front door of the Main Unit and connect the negative lead to the
ground on the R/W/P PROC card.
b. With the positive lead, read the RBC, WBC and PLT test points, closing the door
before taking each reading to prevent outside noise interference.
4. Ensure the noise level readings for RBC, WBC and PLT are within the limits specified in
Table A.1-4, RMS Noise Checks - CBC.
5. Close the upper front door of the Main Unit.
6. If you measured the RMS noise on the CBC channels as part of installation, record the
results on the Installation Test Data Log Sheets as directed in Measuring the RMS Noise,
step 2, under Heading 3.7, ADJUSTMENTS AND CALIBRATION.
Diff and Retic RMS Noise Checks
B Service Disk
B True RMS meter
Procedure
1. Ensure the instrument is in the Diff-Latex Calibration mode. (From the Service Options
menu, select Latex Checks tt Diff Latex Calibration.)
2. Press Ë Secondary.
PN 4235961E 4.12-1
RMS NOISE CHECKS
3. Ensure all the instrument doors and panels are closed to prevent outside noise
interference.
4. While the instrument is idle, use a true RMS meter to measure the RMS noise for the DC,
RF, and LS channels on the VCS PROCESSOR card.
a. Open the upper front door of the Main Unit and connect the negative lead to the
ground on the VCS PROCESSOR card.
b. With the positive lead, read the V (DC), C (RF), and S (LS) test points, closing the
door before taking each reading to prevent outside noise interference.
5. Ensure that the noise for each of the channels does not exceed the limits given for the
static conditions listed in Table A.1-20, Noise Checks - Diff and Retic.
ATTENTION: Dirt, debris or bubbles increase the noise readings. Ensure that the sample is clean
and the system is free of bubbles.
6. Cycle a clean sample of diluent in the selected Latex Calibration mode.
7. While the instrument is counting, measure the RMS noise for DC, RF, and LS channels
on the VCS PROCESSOR card.
8. Ensure that the noise for each of the channels does not exceed the limits given for the
dynamic conditions listed in Table A.1-20, Noise Checks - Diff and Retic.
9. If the MAXM analyzer is a Retic analyzer, measure the static and dynamic noise in the
Retic mode:
a. Change the Latex Calibration mode to Retic-Latex Calibration mode. (From the
Service Options menu, select Latex Checks tt Retic Latex Calibration.)
b. Press Ë Secondary.
c. Repeat steps 4 through 8 in the Retic-Latex Calibration mode.
10. Close the upper front door of the Main Unit.
11. If any of the static or dynamic noise measurements are out of specifications, correct the
problem. Go to Heading 7.3, DC, RF, OR LS NOISE PROBLEMS.
12. If you measured the RMS noise on the Diff and Retic channels as part of installation,
record the results on the Installation Test Data Log Sheets as directed in Measuring the
RMS Noise, step 4, under Heading 3.7, ADJUSTMENTS AND CALIBRATION.
13. If you measured the RMS noise on the Diff and Retic channels as part of VCS
optimization, continue with that procedure.
ATTENTION: You check RMS noise on the Diff and Retic channels twice during VCS
optimization, before and after checking the volumes of the Erythrolyse II reagent and
StabiLyse reagent pumps.
r If you are doing this noise check before checking the volumes of the Erythrolyse II
reagent and StabiLyse reagent pumps, check those volumes now as directed under
Heading 4.16, ERYTHROLYSE™ II AND STABILYSE™ REAGENT PUMPS
ADJUSTMENT AND REPLACEMENT.
r If you are doing this noise check as the first step in verifying instrument operation
after checking the volumes of the Erythrolyse II reagent and StabiLyse reagent
pumps, continue with the verification procedure. Go to I. Verification, step 2, under
Heading 4.9, VCS OPTIMIZATION.
4.12-2 PN 4235961E
FLOW-CELL CLEANING 4
4.13 FLOW-CELL CLEANING
Purpose
Perform this procedure:
r Before performing the LASER/FLOW CELL ALIGNMENT procedure, to ensure that the
flow-cell aperture is clean and free of debris.
r When the LS Offset voltage is failing specifications in the Diff or Retic mode.
r When the LS CV% is failing specifications in the Diff or Retic mode.
Note: A dirty flow cell can also contribute to problems with NE RLS Mean, NE RLS SD, NE
PVR, and LATRON control mean and mean-to-mode.
B Isopropyl alcohol (ChromAR), analytical grade 99.5% purity or better, PN 1606138
ATTENTION: Use lint-free paper or gauze to avoid contaminating the alcohol with dust.
B Lint-free paper or gauze

B Applicator stick, PN 2527713
B Tamperproof screwdriver, PN 5415219
B Tape, Scotch type or masking
Procedure
indirectly from reflective surfaces (such as a mirror or shiny metallic surface). To avoid accidental exposure
to the laser beam while cleaning the flow cell, always turn off the instrument before doing this procedure.
1. Power down the instrument as directed in Power Down/Power Up the System under
Heading 4.1, GUIDELINES FOR SERVICING THE MAXM ANALYZER.
2. Using the tamperproof screwdriver, carefully remove the screws that hold the laser
flow-cell cover.
3. Check for leakage around the flow cell aperture area. If a leak exists replace the flow cell.
4. Remove the flow-cell shield from the flow cell and place it in a clean area while cleaning
the flow cell.
5. Secure a piece of transparent tape on the front of the lens-block assembly to avoid
splashing into the lens.
WARNING Personal injury from broken glass. Breaking off the tip of the ampule of alcohol without
protecting your fingers could lead to cuts from broken glass. Cover the ampule with lint-free paper or gauze
to protect your fingers when opening the ampule.
6. Cover the ampule of alcohol with lint-free paper or gauze to protect your fingers and
break off the tip.
PN 4235961E 4.13-1
FLOW-CELL CLEANING
Figure 4.13-1 Wetting Applicator with Alcohol

CAUTION Alcohol can soften the paint used on the
S# u
CA Man flow cell, permanently damaging the flow cell. To avoid
67
damaging the flow cell, ensure the applicator stick

160
does not contact the painted surfaces of the flow cell

O
P/N
PR
ISO
and do not reuse the applicator stick.
7. Carefully moisten the applicator tip with

isopropyl alcohol without over saturating it
(Figure 4.13-1).
5961123D
Figure 4.13-2 Wiping Front of Flow Cell 8. Place the applicator tip flat on the front of
the flow-cell aperture and pull it out
toward the outside of the instrument (in a
one-way motion only). See Figure 4.13-2.
5961122C
Figure 4.13-3 Wiping Rear of Flow Cell 9. Obtain a new applicator stick and repeat
step 7.
10. Place the applicator flat on the rear of the
flow-cell aperture and pull it out toward
the outside of the instrument (in a one-way
motion only). See Figure 4.13-3.
11. Also clean the side of the flow-cell aperture
facing the outside of the instrument.
12. Repeat steps 7 through 11 if necessary.
5961124C
13. Remove the tape from the lens block and install the shield back onto the flow cell.
14. Power up the instrument.
15. Check for correct alignment of the scattered beam on the sensor’s mask.
4.13-2 PN 4235961E
FLOW-CELL CLEANING 4
Verification
1. If you cleaned the flow cell as part of the laser/flow cell alignment procedure, no
additional verification is needed. Leave the laser flow-cell cover off and go back to
2. Reinstall the laser flow-cell cover.
3. Check the laser on current/LS offset voltage as directed in Laser On Current/LS Offset
Voltage Check under Heading 4.23, VOLTAGE CHECKS AND ADJUSTMENTS.
b. On instruments with a BSV with air cylinders, do the BSV and BSV Housing
CHECKS.
e. Run controls.
PN 4235961E 4.13-3
FLOW-CELL CLEANING
4.13-4 PN 4235961E
LENS BLOCK CLEANING 4
4.14 LENS BLOCK CLEANING
Purpose
ATTENTION: Replace any field-cleaned lens block assembly as soon as possible.
Use this procedure only as the last step if you are unable to achieve the LS specifications when
performing the TTM alignment procedure. Processes for assembling this lens block assembly
have been optimized to ensure contamination of the lens surface does not occur, but once
contaminated, cleaned lenses do not stay clean long. Replace a field-cleaned lens block
assembly as soon as possible.
B Lens paper, PN 3814123
B Analytical grade acetone (1 ppm), PN 1615361
B Powderless gloves
Procedure
CAUTION Powder and skin oils will damage the lenses. Clean your hands with an alcohol prep pad or wash
them with soap and water before performing this procedure. Wear powder-free gloves to prevent skin oils
from coming in contact with the lens surfaces.
indirectly from reflective surfaces (such as a mirror or shiny metallic surface). To avoid accidental exposure
to the laser beam while cleaning the lens block, always turn off the instrument before doing this procedure.
2. Using the tamperproof screwdriver, carefully remove the screws that hold the laser
flow-cell cover.
3. Remove the hardware securing the lens block to the TTM.
4. Place a new sheet of lens paper on a clean surface.
5. Cut another piece of lens paper about the same width as the lens or slightly smaller.
6. Carefully loosen the lens cover screws. Loosen the screws 1/4-turn at a time to prevent
breaking the lens.
7. Remove the lenses from the block and place them on the new piece of lens paper.
8. Holding one of the lenses in gloved hand, drape the cut piece of lens paper over the lens
and drop one drop of acetone near the edge of the lens onto the lens paper.
9. Slide the lens paper off the lens slowly sideways, dragging the drop of acetone to the side
of the lens.
10. Drape the same lens paper over the opposite side of the lens and drag it across the lens as
in step 9 above.
11. Look at the acetone drop to verify complete evaporation. If the lens surface remains wet
with acetone, continue dragging lens paper until lens is dry.
PN 4235961E 4.14-1
LENS BLOCK CLEANING
ATTENTION: Do not use compressed air to remove dust particles because moisture and
propellant in the air may contaminate the lens surface, defeating the cleaning process.
12. Look at the cleaned lens surface from the side to determine if all contamination has been
removed. If it has not:
a. Remove dust particles with an aperture brush.
b. If any contamination remains, repeat the cleaning procedure for the surface once.
c. If the second cleaning does not remove the contamination, replace the lens block
assembly.
13. Carefully place the lens in the lens block.
CAUTION Tightening the screws incorrectly damages the lens cover. Tighten the screws alternately to
prevent warping the lens cover. Do not overtighten.
14. Carefully install the lens cover and install the screws. Tighten the screws until the cover
just touches the lens. Continue tightening the screws alternately 1/4-turn at a time.
15. Repeat the procedure for the other lens.
16. Once both lenses have been cleaned, install the lens block in the TTM.
ATTENTION: If after completion of the LASER/FLOW CELL ALIGNMENT procedure, the
instrument fails the Diff and Retic Latex Calibration and Verification procedure for LS,
replace the lens block and repeat the LASER/FLOW CELL ALIGNMENT procedure.
18. Align the laser/flow cell and verify instrument performance as directed under
4.14-2 PN 4235961E
LASER/FLOW CELL ALIGNMENT 4
4.15 LASER/FLOW CELL ALIGNMENT
Purpose
Use this procedure when you cannot obtain acceptable LS results during diff and retic latex
calibration.
Like the overall VCS procedure, the laser/flow-cell alignment procedure is further divided
into several procedures and you may have to work back and forth, repeating adjustments,
until all measurements fall within specifications. See Figure 4.15-1.
Figure 4.15-1 Laser/Flow-Cell Alignment The goal is to achieve:

Work Flow
r Minimum laser on current/LS offset voltage.
Laser/ r Minimum LATRON CVs.
Flow-Cell
Alignment r Minimum LATRON mean-to-mode difference.
procedure
r NE RLS Mean assay value on 5C II cell
control.
Clean r Maximum NE peak-to-valley ratio on whole
flow cell blood and NE peak within 5C assay limits.
r Correct scatterplot distribution and
population positioning for 5-part diff and
Align retics.
Z-axis plate
(focus) r Correct alignment of mechanical
characteristics of TTM assembly.
Align ATTENTION: If you encounter problems performing
X-axis plate
and flow-cell
the laser/flow-cell alignment procedure, ensure the
perpendicularity following components are the current revision
level.
Align
Y-axis (tilt) plate r Flow cell, coated (black painted)
and LS DC r Light shield
phase
r Scatter sensor for MAXM analyzer without
Retics - (LS sensor)
Adjust LS
r Scatter sensor for MAXM analyzer with Retics
gain
- (3-element LS sensor)
r Y-axis (tilt) plate (flow-cell mounting plate)
Check for r Lens block
LS noise r Horizontal bolt plate.
Laser/
Flow-cell
is
aligned 5961214C
PN 4235961E 4.15-1
LASER/FLOW CELL ALIGNMENT
B 20 MHz dual-trace oscilloscope
B Z-axis gauge, PN 5401245
B Dark Current Test Plug, PN 2121422 (only for instruments that do not have an
LS Preamp 5 module)
B Light Shield, PN 5401269
B White paper
Flow-Cell Cleaning
ATTENTION: Always ensure the flow cell is clean before aligning the laser and the flow cell.
Perform the flow-cell cleaning as instructed under Heading 4.13, FLOW-CELL CLEANING.
Flow-Cell Z-Axis (Focus) Alignment

Input Ch. 1 and Ch. 2

Impedance 1M DC
Vertical 500 mV/div.
Horizontal 5 microseconds/div.
Synch Normal - Ch. 1
2. Connect Channel 1 of the scope to the DC (V) channel on the VCS PROCESSOR card.
3. Connect Channel 2 of the scope to the LS (S) channel on the VCS PROCESSOR card.
ATTENTION: Torquing the lock-down screws without shifting the adjustment is very
technique dependent.
4. Loosen, then torque, all alignment lock-down screws, the lens-block screws and the
laser-mount screws. See Figure 4.15-2 for location of the screws. To torque the screws
correctly:
a. Grip the wrench by the knurled portion of the handle. Failure to do so will cause
improper torque.
b. Tighten each screw sequentially, 1/32 to 1/16 turn each time, until torqued.
5. Loosen each set of lock-down screws (X, Y and Z - Figure 4.15-2, C, D, E, F, G, H, I)
equal amounts and just enough to allow adjustments to each axis.
6. To adjust the Z-axis to its preliminary setting, place the 1.360 focus side of the Z-axis
gauge between the center of the tilt plate and the center of the lens block as shown in
Figure 4.15-2 and turn the Z-adjust screw (Figure 4.15-2, A) until the gauge just fits.
4.15-2 PN 4235961E
7. Set up a DMM to measure the laser on current or the LS offset voltage.
Note: Laser on current readings are proportional to LS offset voltage readings.
a. If the MAXM analyzer has an LS Preamp 5 module:
1) Set the DMM to measure volts.
2) Connect the DMM to TP1 and TP3 on the LS Preamp 5 module.
b. If the MAXM analyzer has an LS Preamp 3 module:
1) Disconnect the leads of the LS sensor from the LS Preamp 3 module.
2) Connect the Dark Current Test Plug to the LS sensor leads.
3) Connect the DMM to the Dark Current Test Plug.
4) Set the DMM to read µA.
5) After reading the current, disconnect the LS sensor leads from the Dark
Current Test Plug and reconnect them to the LS Preamp 3 module.
ATTENTION: As you do the rest of this procedure, repeat step 7 b above whenever you need to
read the laser on current.
Figure 4.15-2 Flow-Cell Z-Axis Alignment
Y-AXIS PLATE
(TILT PLATE)
J Y-ADJUST
LENS
BLOCK
I Y-LOCK DOWN
Z-AXIS
GAUGE
H Y-LOCK DOWN
X-AXIS PLATE
G X-LOCK DOWN
A Z-ADJUST
HORIZONTAL
BOLT PLATE
F X-LOCK DOWN
B X-ADJUST
E Z-LOCK DOWN
D Z-LOCK DOWN C Z-LOCK DOWN
Note: The Y-LOCK DOWN screws (H and I) may look different depending on the revision level of the hardware. 5961281D
8. Cycle a sample of LATRON control in the Diff-Latex Calibration mode.

9. Adjust X- and Y-adjust screws (Figure 4.15-2, B and J) to obtain an LS pulse on the
scope.
PN 4235961E 4.15-3
10. Remove the flow cell cover, shine a light on the LS sensor and ensure that the scattered
laser beam is centered vertically on the sensor’s mask and that it is free of dust or other
foreign material. See Figure 4.15-3.
Figure 4.15-3 Scatter Sensor Laser Diffraction

Laser LS Sensor
beam sensor mask
Laser diffraction
in center of white
mark
5961280D
11. Reinstall the flow-cell cover.

12. Using the X- and Y-adjust screws, fine tune the X-axis for maximum LS amplitude with
minimum variations; then turn the Y-adjust screw until you achieve minimum laser on
current (LS Preamp 3 module) or LS offset voltage (LS Preamp 5 module).
13. Note the laser on current or LS offset voltage.
14. Turn the Z-adjust screw clockwise 1/4 to 1/2 turn.
15. Repeat steps 12 through 14.
r If the laser on current or LS offset voltage decreases, continue repeating steps 12
through 14 until the current or offset voltage starts to increase.
r If the laser on current or LS offset voltage increases, turn the Z-adjust screw
counterclockwise to set the XY assembly past the preliminary setting, then repeat
steps 12 through 14.
16. Select QUIT to stop the LATRON control flow through the flow cell.
17. Torque the Z-lock down screw C (refer to Figure 4.15-2), then loosen it again. This
squares up the XY assembly before final torquing of the Z-axis.
18. Tighten each Z-lock down screw (Figure 4.15-2, C, D, E) sequentially, a little at a time,
until torqued.
Flow-Cell X- and Y-Axis Plate Alignment

1. Cycle a sample of LATRON control in the Diff-Latex Calibration mode.
2. Using the X- and Y-adjust screws (Figure 4.15-4, B, J), fine tune the X-axis for maximum
LS amplitude with minimum variations; then adjust the Y-axis screw until you achieve
minimum Laser On Current/LS Offset Voltage.
ATTENTION: When torquing the Y-(vertical) lock down screws, a SLIGHT change in alignment
is acceptable at this time, but you must ensure that it does not change drastically.
4.15-4 PN 4235961E
3. Torque the Y-lock down screws (Figure 4.15-4, H, I).
4. Remove the flow-cell cover and the lens-block assembly.
Figure 4.15-4 Flow-Cell X- and Y-Axis Plate Alignment

TOP VIEW
TILT PLATE
Y-LOCK DOWN
X-LOCK DOWN
J
Y-ADJUST
(VERTICAL
ADJUSTMENT)
Y-AXIS PLATE
(TILT PLATE)
X-AXIS PLATE Y-ADJUST FLOW CELL
I Y-LOCK DOWN
H Y-LOCK DOWN
G X-LOCK DOWN
B X-ADJUST
HORIZONTAL
BOLT PLATE
F X-LOCK DOWN
Note: The Y-LOCK DOWN screws (H and I) may look different depending on the revision level of the hardware. 5961282D
5. Remove the light shield from the flow cell and insert the special light shield.
6. Pull the light shield out and adjust its position until the laser’s main beam just touches
the far side of the hole and the reflected beam is at its brightest. Ensure the laser’s
reflected beam is centered on the vertical white line directly below the main beam. See
Figure 4.15-5.
a. If the reflected beam is not centered, loosen and then retorque the Z-lock down
screws (refer to Figure 4.15-2, D, E).
b. If the reflected beam cannot be aligned, ascertain which parts are defective, replace
the parts and perform the laser alignment procedure from the beginning.
7. Without shifting the reflected beam off the vertical white line, tighten each
X-(horizontal) lock-down screw (refer to Figure 4.15-4, F, G) sequentially, a little at a
time, until they are torqued.
8. Remove the special light shield from the flow cell and reinstall the regular light shield.
9. Reinstall the lens-block assembly on its positioning pin on the laser mount and torque
into place.
10. Visually realign the flow cell.
11. Reinstall the flow-cell cover.
PN 4235961E 4.15-5
Figure 4.15-5 Laser Reflected Light Beam with Special Light Shield Installed
Lens
block
assembly
Special
light
shield
Laser
GOOD BAD
5961283D
Flow-Cell Y-Axis Final Alignment

ATTENTION: This is the final step in the physical alignment of the laser to the flow cell.
Alignment of Y-axis (tilt) plate affects the laser on current/LS offset voltage, LATRON CVs
and mean-to-mode, and 5C II cell control NE RLS MN and NE PVR. You may need to readjust
the X- or Z-axis plates to find the best alignment of the Y-axis plate.
1. Loosen the Y-(vertical) lock down screws (Figure 4.15-4, H, I) just enough to allow the
Y-axis (tilt) plate to be adjusted.
2. Adjust the Y-(vertical) adjust screw (Figure 4.15-4, J) for minimum laser on current/LS
offset Voltage.
3. Without shifting the laser alignment, tighten each Y-lock down screw (Figure 4.15-4, H,
I) sequentially, a little at a time, until the screws are torqued.
4. Verify that the laser on current/LS offset voltage is still within range.
5. It the laser on current/LS offset voltage is not within range:
a. Reclean the flow cell as directed under Heading 4.15, LASER/FLOW CELL
ALIGNMENT and realign the TTM as directed in this section.
b. Replace the flow cell and realign the TTM as directed in this section.
4.15-6 PN 4235961E
c. Replace the lens block and realign the TTM as directed in this section.
d. As a last resort if a new lens block is not immediately available, clean the current
lens block as directed under Heading 4.14, LENS BLOCK CLEANING and realign
the TTM.
6. Run a LATRON control in the Diff-Latex Calibration mode. Verify that:
a. LS CVs are within range. If not, realign the X-axis; go to Flow-Cell X- and Y-Axis
Plate Alignment.
b. LS Mean-to-Mode differences are within range. If not -
1) Check for noise, leaks, bubbles or contamination.
2) If the problem persists, replace the flow cell.
7. Remove the flow-cell cover, shine a light on the LS sensor and ensure that the scattered
laser beam is centered vertically on the sensor’s mask. Refer to Figure 4.15-3.
8. Slide a white piece of paper between the flow cell’s aperture and the LS sensor. Ensure
that the diffracted laser beam is a fine line without any vertical lines and that the cones
are minimized. See Figure 4.15-6.
Figure 4.15-6 Laser Diffraction Pattern on Paper
9. Remove the paper, ensuring that no lint is left behind.

10. Reinstall the flow-cell cover and secure it with its mounting screws.
11. If the laser alignment shifts after tightening the flow-cell cover, check the following for
proper fitting:
r Screws holding the laser to the base plate
r Coaxial cable
r Flow-cell tubing bracket
r Flow-cell cover
Verification
Verify the RF, DC and LS gains settings are correct. Go to Diff and Retic Latex Calibration and
PN 4235961E 4.15-7
4.15-8 PN 4235961E
ERYTHROLYSE™ II AND STABILYSE™ REAGENT PUMPS ADJUSTMENT AND REPLACEMENT 4
REPLACEMENT
Erythrolyse II and StabiLyse Reagent Pumps Adjustment
Purpose
The adjustment of the Erythrolyse II and StabiLyse reagent pumps optimizes count ratio and
cell positioning by varying the conductivity and osmolality of the final dilution.
The count ratio can be degraded by a mismatch between the conductivity of the sheath fluid
(diluent) and the conductivity of the sample stream (blood + Erythrolyse II reagent +
StabiLyse reagent) in the flow cell. See Figure 4.16-1. You can vary the conductivity of the
sample by changing the volume of Erythrolyse II and StabiLyse reagents in the dilution.
Varying the StabiLyse reagent pump volume has a greater affect on the final sample
conductivity than varying the Erythrolyse II reagent pump by the same volume.
Figure 4.16-1 Match Sheath Fluid to Sample Stream Conductivity
Conductivity of sheath fluid = Conductivity of blood + Erythrolyse ll reagent + StabiLyse reagent
FLOW CELL
Sheath-fluid in
Sheath-fluid in
5961271D
Sample in
The correct positioning of cell populations within the VCS "cube" is dependent upon the
osmolality of the dilution. Varying the Erythrolyse II reagent pump volume varies the
osmolality of the dilution.
PN 4235961E 4.16-1
ERYTHROLYSE™ II AND STABILYSE™ REAGENT PUMPS ADJUSTMENT AND REPLACEMENT
Figure 4.16-2 Effect of Dilution Conductivity on In the following procedure:

Count Ratio
r To obtain the best count ratio, adjust
8000 the StabiLyse reagent pump volume
until you obtain the center value of the
6000
count ratio plateau. See Figure 4.16-2.
4000
2000
CONDUCTIVITY
COUNT RATIO 5961210C
Figure 4.16-3 Effect of Osmolality on NE DC mean r To obtain the best cell positioning,
adjust the Erythrolyse II reagent pump
170 volume until you obtain the 5C II
160
Normal cell control assay value for the
150
NE DC Mean. See Figure 4.16-3.
140
130
120
110
100
OSMOLALITY
5C II CELL CONTROL NE DC MEAN 5961211C
Note: Reagent "potency" due to aging and temperature also affects conductivity and cell
positioning. This procedure optimizes the instrument to the current reagents and
temperature.
Since count ratio and cell positioning are both affected by the volume of the Erythrolyse II
reagent and the StabiLyse reagent pumps, you must adjust both pumps back and forth until
you find the ideal settings for both. Figure 4.16-4 is a flow diagram for optimizing the pump
adjustments.
B ACCUVETTE II vial or equivalent
B Service Disk
B Volumetric Cylinder, PN 5415374
B Normal whole blood specimen mixed for at least 30 minutes before using. See the
Specimen Collection section of the customer’s Operator’s Guide for anticoagulant and
storage specifications.
B 5C Normal cell control with 5C II cell control assay sheet (if available)
4.16-2 PN 4235961E
Figure 4.16-4 Erythrolyse II Reagent and StabiLyse Reagent Pump Optimization Flow Diagram
Set Erythrolyse Il reagent

pump volume to
within limits on
calibrated vial.
Find lower StabiLyse reagent

pump volume by
monitoring count
ratio on normal blood
sample.
Find upper StabiLyse reagent

pump volume by
monitoring count
ratio on normal blood
sample.
Adjust StabiLyse reagent

pump to middle
of range.
Run 5C Normal control

and note NE DC mean
value recovered.
Is NE DC NO Adjust Erythrolyse Il Is count NO

mean within reagent pump volume ratio still
assayed range to achieve NE DC good
? mean value. ?
YES YES
Adjust StabiLyse reagent pump

5 µL in opposite direction
Erythrolyse ll reagent pump
was adjusted and rerun
normal blood sample.
Is count NO
ratio still
good
?
YES
Setting
of pump Adjust StabiLyse reagent
volume is pump back 5 µL.
complete.
5961272D
PN 4235961E 4.16-3
Adjusting the Erythrolyse II Reagent Pumps Volume
Figure 4.16-5 Tubing from Mixing Chamber 1. Disconnect the Erythrolyse II reagent tubing from
the mixing chamber. See Figure 4.16-5.
Disconnect from
mixing chamber 2. Place the tube into an ACCUVETTE II vial.
3. From the Service Options menu, select Solenoid
Test tt Í Cycles tt Erythrolyse Pump Dispense to
activate the Erythrolyse II reagent pumps. Ensure
the tubing is primed with Erythrolyse II reagent.
4. Touch the tube against the side of the vial to
remove the drop from the end then remove the
tube from the vial.
5961205C
Figure 4.16-6 Volumetric Cylinder Calibration Marks 5. Place the tube in the Volumetric Cylinder.
6. From the Service Options menu, select Solenoid
Calibration
Test tt Í Cycles tt Erythrolyse Pump Volume
Erythrolyse II reagent Test (X5) to activate the Erythrolyse II reagent
marks
pumps five times, dispensing Erythrolyse II
Pump reagent five times into the Volumetric Cylinder.
volume 7. Hold the Volumetric Cylinder in a vertical
acceptable
position and ensure that the meniscus is between
Pump the minimum and maximum markings for
volume
acceptable Erythrolyse II reagent. See Figure 4.16-6.
Calibration StabiLyse reagent

marks
5961206C
4.16-4 PN 4235961E
Figure 4.16-7 Reading the Meniscus 8. Take the reading at the bottom of the meniscus as
shown in Figure 4.16-7.
MENISCUS
Meniscus
READ
Read
HERE
here
M
A
X
M
5961207C
5961207D
Figure 4.16-8 Diaphragm Pump Adjustment 9. If the meniscus is not between the markings:
DIAPHRAGM-STYLE a. Adjust the pump. See Figure 4.16-8.
PUMP
Note: Two Erythrolyse II reagent pumps are
used to dispense the total reagent volume.
You can adjust either pump to change the
5 µL 1/8 TURN volume.
ATTENTION: After each measurement, empty the
Volumetric Cylinder and dry with a cotton swab.
5961208C
b. Empty and dry the Volumetric Cylinder.
c. Repeat steps 2 through 8.
10. Reattach the tubing disconnected from the
mixing chamber in step 1.
Adjusting the StabiLyse Reagent Pump Volume

ATTENTION: An example of analyzing data to determine the StabiLyse reagent pump
adjustment follows this procedure. Refer to the example if you need clarification on analyzing
the data.
1. Use the Service Disk to obtain enhanced printouts as directed in Activating the DMS
Service Options under Heading 4.2, USING THE SERVICE DISK.
2. Run a sample of the well-mixed (30 minutes) normal whole-blood specimen as a patient
sample under Sample Analysis so that it goes into the DMS database and record the
count ratio.
Note: To make data analysis easier, record the information in a table like the one shown
in the example box that follows.
PN 4235961E 4.16-5
3. Decrease the StabiLyse reagent pump volume in 5-µL increments (refer to

Figure 4.16-8), running blood and recording the count ratio after each adjustment, until
the count ratio begins to degrade.
4. Return the pump volume to its original setting.
5. Increase the StabiLyse reagent pump volume in 5-µL increments, running blood and
recording the count ratio after each adjustment, until the count ratio begins to
degrade again.
6. Referring to the example box below as needed, determine the final pump adjustment:
a. Find the amount the pump volume was decreased before count ratio degradation.
(Minimum)
b. Find the amount the pump volume was increased before count ratio degradation.
(Maximum)
c. Add the values from step a and step b and divide the sum by two.
Minimum + Maximum
------------------------------------------------------- = Adjustment
2
7. Adjust the pump back to the maximum pump volume where the count ratio was still
good. Then adjust the pump down the amount determined in step 6 c.
Note: The final adjustment of the pump should always be half way between the upper
(maximum) and lower (minimum) pump-volume limits where the count ratios are
still good.
Determining the StabiLyse Reagent Pump Volume Adjustment, Example Data
1. The amount decreased before count

Run # Pump Adjust Diff from Start Count Ratio degradation is 10 µL (run #3). [Minimum]
1 Start Start 8012 2. The amount increased before count
degradation is 20 µL (run #10). [Maximum]
2 ↓ 5 µL ↓ 5 µL 8004
3. (10 µL + 20 µL) ÷ 2 = 15 µL [(Min)+(Max)/2]
3 ↓ 5 µL ↓ 10 µL 7997
4. Adjust the pump volume to ↑ 20 µL from the
4 ↓ 5 µL ↓ 15 µL 6871 Start setting, then adjust the volume ↓ 15 µL.
5 ↓ 5 µL ↓ 20 µL 3217 The final setting should be ↑ 5 µL from Start
as shown in this figure.
6 Return to Start 8117
30
Start
25
7 ↑ 5 µL ↑ 5 µL 8009 20
15
8 ↑ 5 µL ↑ 10 µL 7984 10
µL
9 ↑ 5 µL ↑ 15 µL 7989 5
0
10 ↑ 5 µL ↑ 20 µL 7992 -5
-10
11 ↑ 5 µL ↑ 25 µL 5798 -15
12 ↑ 5 µL ↑ 30 µL 2087 -20
1 2 3 4 5 6 7 8 9 10 11 12 13
13 Final From Start 8017 Pump Adjust Lower Limit Upper Limit
Adjust (Min+Max)/2
5915414H
4.16-6 PN 4235961E
Optimizing the Erythrolyse II Reagent Pumps Volume
ATTENTION: Do this procedure as the final adjustment of the Erythrolyse II reagent pumps if
5C Normal cell control assayed for service parameters is available.
1. Use the Service Disk to obtain enhanced printouts as directed in Activating the DMS
Service Options under Heading 4.2, USING THE SERVICE DISK.
2. Run 5C Normal cell control in the Primary mode with the bar-code label turned away
from the bar-code reader so that the results are stored in the database.
3. Compare the value of the NE DC Mean channel printed on the enhanced printout to the
value on the assay sheet.
r If the value recovered is within the assay range, proceed to Verifying Erythrolyse II
and StabiLyse Reagent Pumps Volume Adjustment.
r If the value recovered is outside the assay range, go to step 4.
ATTENTION: For every channel of NE DC Mean change desired, adjust the pump volume
approximately 5 µL. Refer to Figure 4.16-8.
4. Adjust the Erythrolyse II reagent pump, noting the direction and amount of adjustment,
then rerun the control.
r If the value recovered is below the assay range, increase the reagent volume.
r If the value recovered is above the assay range, decrease the reagent volume.
5. Continue adjusting the Erythrolyse II reagent pump and running the control until the
value recovered for NE DC Mean is within the assay range.
6. Check the count ratio.
r If the count ratio is still acceptable, go to step 8.
r If the count ratio has degraded as a result of adjusting the Erythrolyse II reagent
pump, go to step 7.
7. Readjust the Erythrolyse II reagent and StabiLyse reagent pumps:
a. Set the Erythrolyse II reagent pump as close as possible to achieving the NE DC
Mean assay.
b. Go back to Adjusting the StabiLyse Reagent Pump Volume, and repeat that
procedure using the new Erythrolyse II reagent pump volume as a starting point.
c. Repeat this procedure, starting at step 2.
8. Adjust the StabiLyse reagent pump volume 5 µL in the opposite direction from the
adjustment on the Erythrolyse II reagent pump volume.
Note: Since the Erythrolyse II reagent volume has some affect on the final dilution
conductivity, perform this adjustment to ensure the new volume does not bring the
count ratio too close to the edge of the "count ratio plateau."
9. Rerun the normal whole-blood specimen used for setting the StabiLyse reagent pump
volume and note the count ratio.
r If the count ratio is still good (>7750), reset the StabiLyse reagent pump back 5 µL
to the original setting. The pump optimization is complete.
r If the count ratio is degraded, repeat from step 2, starting with the new
Erythrolyse II pump setting.
PN 4235961E 4.16-7
Verifying Erythrolyse II and StabiLyse Reagent Pumps Volume Adjustment

Verify the pump volumes are set for optimal whole blood results as directed in I. Verification
under Heading 4.9, VCS OPTIMIZATION.
Erythrolyse II Reagent Pumps Replacement
Erythrolyse II Reagent Pumps Removal

2. Remove the tubing from both the top and bottom fittings of the Erythrolyse II reagent
pumps.
3. Remove the two Phillips-head screws that secure the pumps’ mounting bracket to the
Pump module’s panel (Figure 4.16-9 or Figure 4.16-10).
4. Remove the pumps.
Figure 4.16-9 Pump Module Mounting Bracket, Figure 4.16-10 Pump Module Mounting Bracket,
Old Configuration New Configuration
Screw Screw
Pump Pump
mounting mounting
bracket
bracket
5961292D
5961044D
Erythrolyse II Reagent Pump Installation

1. Install the new pumps on the pump mounting bracket.
2. Secure the pump mounting bracket to the Pump module panel with the two
Phillips-head screws.
3. Connect the tubing to the top and bottom fittings of the pumps.
5. From the Main menu select Diluter Functions tt Prime Reagents tt PAK, and observe the
pump and associated tubing for leaks or bubbles.
IMPORTANT New Erythrolyse II reagent pumps may change the dilution ratios, requiring optimization.
Check the volume dispensed by the new pumps.
6. Adjust the volume of the Erythrolyse II reagent pumps and verify that adjustment. Refer
to Erythrolyse II and StabiLyse Reagent Pumps Adjustment at the beginning of
this section.
4.16-8 PN 4235961E
ASPIRATION PUMP VOLUME ADJUSTMENT 4
4.17 ASPIRATION PUMP VOLUME ADJUSTMENT
B Enhanced 5C Normal cell control, all levels, or 4C PLUS cell control, Abnormal High
and Abnormal Low, if Enhanced 5C cell control is not available
B Five different whole blood specimens
Procedure
1. In the Primary mode, run a sample of Enhanced 5C Normal cell control or 4C PLUS
Abnormal High cell control.
2. Observe the aspiration line at the front blood/bubble detector BD1-2 (Figure 4.17-1).
Figure 4.17-1 Second Pull (Vacuum)
Blood FF44
Air BSV
Front Rear
blood blood
detector detector
X 2 1 3 12 2 1
BD1 BD2
Tygon tubing Tygon tubing Tygon tubing

7.5 cm (3 in.) 5 cm (2 in.) 8.9 cm (3.5 in)
Tygon® tubing
42 cm (16.5 in.)
5961138D
3. Ensure that on the second pull (the vacuum pull) of the sample, the distance X between
the trailing edge of the sample and the end of the fitting of BD1-2 is within the tolerance
specified in Table A.1-24, Aspiration Pump Volume Tolerances.
r If X is within tolerance, go to step 5.
r If X is not within tolerance, adjust the pump. Go to step 4.
4. Adjust the aspiration pump. Turn the top of the pump:
r Clockwise to decrease the volume [shorten the distance (X)]
r Counterclockwise to increase the volume [lengthen the distance (X)].
Note: When adjusting the pump, rotate the top back and forth a few times while turning
to properly seat the pump diaphragm.
5. Repeat steps 1 through 3 four times and verify that any differences between the distance
X measurements are within the tolerance specified for replicates in Table A.1-24,
Aspiration Pump Volume Tolerances.
r If the replicate measurements for X are within tolerance, go to step 6.
r If the replicate measurements for X are not in tolerance, troubleshoot the aspiration
system, and repeat pump verification after resolving the problem.
PN 4235961E 4.17-1
ASPIRATION PUMP VOLUME ADJUSTMENT
6. Run all levels of control and five different patient specimens and ensure that on the first
pull (aspiration pump pull) of the sample, blood does not enter the front blood/bubble
detector (Figure 4.17-2). If the blood samples enter the front blood/bubble detector on
the first pull:
r Troubleshoot the aspiration system.
r Repeat pump verification after resolving the problem.
Figure 4.17-2 First Pull (Aspiration Pump))
FF44
FF44
Blood
BLOOD
Air
AIR
Front Rear
FRONT REAR
Diluent
DILUENT blood
BLOOD blood
BLOOD
DETECTOR
detector DETECTOR
detector
Diluent
DILUENT
2 2 11 33 12
12
12
12 22 11
BD1
BD1 BD2
BD2
BUBBLE
Bubble BSV
BSV
3 in. (7.5
Tygon cm)
tubing 2 in.tubing
Tygon (5 cm) 3 in.tubing
Tygon (7.5 cm)
7.5 cmTYGON
(3 in.) 5 cmTYGON
(2 in.) 8.9 cm TYGON
(3.5 in.)
16.5 in. (42 cm) TUBING TUBING TUBING
Tygon tubing
TYGON
42TUBING
cm (16.5 in.)
Blood
BLOOD 5961139D
5961139C
Verification
1. If you did this procedure following replacement of the BSV, check the diluent delivery
timing. Go to Heading 4.20, LYTIC REAGENT AND DILUENT DELIVERY TIMING
CHECK.
2. If you adjusted the aspiration pump, verify calibration.
a. Do Heading 4.7, INITIAL ADJUSTMENT OF PRIMARY-MODE CALIBRATION
FACTORS.
b. Advise the customer that you have changed the calibration and that they need to
verify calibration of the Primary mode as outlined in the Special Procedures and
Troubleshooting manual.
CHECKS.
e. Run controls.
4.17-2 PN 4235961E
CBC LYTIC REAGENT PUMPS VERIFICATION 4
4.18 CBC LYTIC REAGENT PUMPS VERIFICATION
B ACCUVETTE II vial or equivalent
B Scale accurate within 0.5 g
B Clear, polyurethane tubing, 0.082 i.d., PN 3202036
Procedure
1. Weigh a clean, dry, empty vial and record the weight for future reference.
2. Ensure that the CBC lytic reagent pumps are primed.
3. Disconnect the CBC lytic reagent tubing from FF1 by PV1.
4. Connect 15 cm (6 in.) of the clear, polyurethane tubing to FF1.
6. From the Service Options menu, select Solenoid Test.
7. Place the other end of the tubing attached to FF1 into the weighed vial and energize LV4
twelve times to dispense CBC lytic reagent into the vial twelve times.
8. Weigh the vial of CBC lytic reagent and subtract the weight recorded in step 1 to
determine the weight of the reagent.
9. Ensure that the weight of the lytic reagent is within the tolerances specified for Volumes,
CBC Lytic Reagent Pumps’ Volume Tolerance, under Heading A.1, TOLERANCES AND
LIMITS.
r If the weight is not within tolerance, replace both CBC lytic reagent pumps and
repeat from step 1.
r If the weight is within tolerance, go to step 10.
10. Remove the tubing installed in step 4 and reconnect the original tubing at FF1.
11. Prime the lytic reagent.
Verification
IMPORTANT Because the CBC lytic reagent pumps measure and dispense the lytic reagent used in the WBC
dilutions, changing a CBC lytic reagent pump can affect the instrument results. Always check the CBC lytic
reagent delivery timing and the instrument calibration after replacing a CBC lytic reagent pump.
1. If you did this procedure following replacement of a CBC lytic reagent pump, check the
CBC lytic reagent and diluent delivery timing. Go to Heading 4.20, LYTIC REAGENT
AND DILUENT DELIVERY TIMING CHECK.
PN 4235961E 4.18-1
CBC LYTIC REAGENT PUMPS VERIFICATION

CHECKS.
e. Run controls.
4.18-2 PN 4235961E
RBC/WBC DILUENT DISPENSERS VOLUME TESTING 4
4.19 RBC/WBC DILUENT DISPENSERS VOLUME TESTING
Purpose
Use the procedures in this section to test the volume dispensed by the RBC and WBC diluent
dispensers, and to adjust the diluent dispensers if necessary. Two methods for determining the
volume dispensed are provided, Measuring Diluent by Volume and Measuring Diluent by
Weight.
Measuring by volume requires a graduated cylinder; measuring by weight requires a scale.

The method you choose depends on which tool is available in your customer’s laboratory and
personal preference. Measuring by weight is easier because you do not have to read a
meniscus like you do when measuring by volume. Measuring by weight is also quicker
because you dispense diluent only once instead of the 10 times required for measuring by
volume.
The basic procedures for measuring the volumes for the RBC and WBC diluent dispensers are
the same. The only differences are:
r The tubing you disconnect (FF26 for the RBC diluent dispenser, FF27 for the WBC).
r The amount of total volume required after dispensing the diluent 10 times (refer to
Table A.1-25, Diluent Dispenser Volume Specifications and Tolerances).
Measuring Diluent by Volume
B Service Disk
B Hemostats
B One vial, such as ACCUVETTE® II vial, for waste
B Graduated cylinder, >100 mL
Procedure
1. Ensure that the instrument and the DMS are ON.
3. Ensure the graduated cylinder is clean and dry.
4. Ensure the diluent dispensers are primed.
5. Pinch the pressure tubing to the appropriate diluent dispenser (RBC or WBC) with
hemostats, as necessary.
6. Disconnect the tubing from the appropriate connection on the CBC module:
r For the RBC diluent dispenser - FF26.
r For the WBC diluent dispenser - FF27.
7. Connect 15 cm (6 in.) of tubing to the open connection:
PN 4235961E 4.19-1
RBC/WBC DILUENT DISPENSERS VOLUME TESTING

9. Place the other end of the tubing attached to FF26 or FF27 in step 7 into a waste vial,
and energize LV35 to prime the tubing with diluent.
10. Move the tubing from the waste vial into the graduated cylinder and energize LV35 10
times to dispense diluent into the graduated cylinder:
11. Determine the volume of the diluent from the position of the meniscus.
12. Verify the diluent volume is within the tolerances listed for Measured by Volume in
Table A.1-25, Diluent Dispenser Volume Specifications and Tolerances.
Figure 4.19-1 Reading a Meniscus
EYE LEVEL
Read here
7253210A
13. If the diluent volume is not within the tolerance, adjust the appropriate diluent dispenser
or replace it if necessary, and repeat steps 3 through 11.
14. Disconnect the test tubing from FF26 (RBC diluent dispenser) or FF27 (WBC diluent
dispenser) on the CBC module, and reconnect the original tubing:
15. Remove the hemostats installed in step 5.
16. If you want to test both diluent dispensers, repeat steps 3 through 15 for the other
diluent dispenser.
Measuring Diluent by Weight
B Service Disk
B Hemostats
B Two vials, such as ACCUVETTE II vials
B Scale accurate within 0.05 g
Procedure
3. Weigh a clean, dry, empty vial and record the weight.
4. Ensure that the diluent dispensers are primed.
5. Pinch the pressure tubing to the appropriate diluent dispenser (RBC or WBC) with
hemostats, as necessary.
4.19-2 PN 4235961E
RBC/WBC DILUENT DISPENSERS VOLUME TESTING 4
6. Disconnect the tubing from the appropriate connection on the CBC module:
7. Connect 15 cm (6 in.) of tubing to the open connection:
9. Place the other end of the tubing attached to FF26 or FF27 in step 7 into a waste vial,
and energize LV35 to prime the tubing with diluent.
10. Move the tubing from the waste vial into the weighed vial and energize LV35 to dispense
diluent into the vial.
11. Weigh the vial of diluent and subtract the weight of the vial recorded in step 3, to
determine the weight of the diluent.
12. Verify the diluent weight is within the tolerances listed for Measured by Weight in
Table A.1-25, Diluent Dispenser Volume Specifications and Tolerances.
13. If the diluent weight is not within the tolerance, adjust the appropriate diluent dispenser
or replace it if necessary, and repeat steps 3 through 11.
14. Disconnect the test tubing from FF26 (RBC diluent dispenser) or FF27 (WBC diluent
dispenser) on the CBC module, and reconnect the original tubing:
15. Remove the hemostats installed in step 5.
16. If you want to test both diluent dispensers, repeat steps 3 through 15 for the other
diluent dispenser.
Verification
IMPORTANT Because the diluent dispensers measure and dispense the diluent used in the RBC and WBC
dilutions, changing a diluent dispenser can affect the instrument results. Always check the instrument
calibration after replacing an RBC diluent dispenser, and check both the diluent delivery timing and the
instrument calibration after replacing a WBC diluent dispenser.
1. If you did this procedure following replacement of an RBC diluent dispenser, verify
calibration.
FACTORS.
2. If you did this procedure following replacement of a WBC diluent dispenser, check the
CBC lytic reagent and diluent delivery timing. Go to Heading 4.20, LYTIC REAGENT
AND DILUENT DELIVERY TIMING CHECK.
PN 4235961E 4.19-3
RBC/WBC DILUENT DISPENSERS VOLUME TESTING

CHECKS.
e. Run controls.
4.19-4 PN 4235961E
LYTIC REAGENT AND DILUENT DELIVERY TIMING CHECK 4
4.20 LYTIC REAGENT AND DILUENT DELIVERY TIMING CHECK
B Storage scope with probes
B Coaxial cable with BNC connectors
B Pressure transducer and test box, PN 2906535, or a digital pressure meter, such as a
Marsh Meter, PN 2906639
B Hemostat
Purpose
Use this procedure to verify the lytic reagent and diluent delivery time:
r At installation.
r If the instrument exhibits any of the following conditions -
t Increased differential flagging, "false positives"
t Decreased differential flagging, "false negatives"
t An abnormal number of "incomplete computations" (....) on differential results.
r Whenever you replace a component that could affect the timing, such as -
t WBC diluent dispenser
t Lytic reagent pumps
t Blood sampling valve.
Procedure
ATTENTION: If you are checking the lyse and diluent delivery timing as part of instrument
installation, you do not need to check the volume dispensed by the CBC lytic reagent pumps
and the WBC diluent dispenser. Begin at step 3.
1. Verify that the CBC lytic reagent pumps are delivering the correct volume as directed
under Heading 4.18, CBC LYTIC REAGENT PUMPS VERIFICATION.
2. Verify that the WBC diluent dispenser is delivering the correct volume as directed under
Heading 4.19, RBC/WBC DILUENT DISPENSERS VOLUME TESTING.
CAUTION Connecting or disconnecting trigger cables to the DILUTER INTERFACE card with the power on
can damage the card. Turn off the instrument before connecting and disconnecting trigger cables to the
DILUTER INTERFACE card.
4. Disconnect the Y-fitting for the CBC lytic reagent and diluent input line to the WBC
bath. See Figure 4.20-1.
5. Connect the external trigger probe to LYSE TRIG on the DILUTER INTERFACE card
and the trigger probe ground to chassis ground.
6. Connect the transducer and the test equipment as shown in Figure 4.20-2 or
Figure 4.20-3, ensuring there are no crimps or pinched tubing.
PN 4235961E 4.20-1
LYTIC REAGENT AND DILUENT DELIVERY TIMING CHECK
Figure 4.20-1 WBC Bath Tubing Disconnect for Lytic Reagent/Diluent Delivery Timing Hookup
Disconnect
FF27 Y-fitting from
here
WBC
bath
5961268D
Figure 4.20-2 Test Box and Transducer Hookup for Lytic Reagent/Diluent Timing Procedure
Storage
scope
Trigger
probe
DILUTER INTERFACE card
Chassis ground
ON
WBC bath
OFF
+
-
Output Sensor
FF27
Transducer
5961266D
4.20-2 PN 4235961E
LYTIC REAGENT AND DILUENT DELIVERY TIMING CHECK 4
Figure 4.20-3 Digital Pressure Meter Hookup for Lytic Reagent/Diluent Timing Procedure
Storage
scope
Trigger
probe
DILUTER INTERFACE card
Chassis ground
WBC bath
Digital
Pressure
Meter
FF27
5961267D
7. Configure scope settings as shown:

.
Function Setting
Source DC
Timebase 0.5 sec/div
Capture Single and hold
Trigger Negative external trigger
Channel Single channel
Voltage 20 mV, 50 mV or 0.1 V/div
AC-DC-GD DC

9. Adjust the 30-psi pressure to 30 psi ±0.1.
10. Prime the transducer to eliminate bubbles in the tubing.
a. From Sample Analysis menu, press Ð Rinse which drains and rinses baths.
b. Unclamp hemostats when baths are filling to prime sensor.
c. Place gauze or a vial under tubing to catch diluent.
d. Clamp hemostats during diluent flow to prevent bubbles.
e. Repeat steps a through d as necessary until lines are bubble-free.
11. Cycle the instrument in the Secondary mode to prime.
PN 4235961E 4.20-3
LYTIC REAGENT AND DILUENT DELIVERY TIMING CHECK
12. Cycle the instrument in the Secondary mode again to display a waveform on the scope.
13. Compare the waveform display to the example in Figure A.1-1 and verify that the timing
of the waveform is within the given tolerances.
Note: T0 - T4 should be as close to the nominal value specified in Figure A.1-1 as
possible to stay within the specifications if the 30-psi pressure increases or decreases.
14. If the timing is not correct:
a. Change or adjust the choke according to Table A.1-16, Diluent and Lyse Timing
Acceptable Choke Combinations
b. Repeat from step 12 until the tolerances are met.
16. Disconnect the trigger cables and transducer hookup.
17. Reconnect the Y-fitting to the CBC lytic reagent and diluent input line for the WBC bath.
18. Power up the instrument and verify it is working correctly. Go to Verification.
Verification
1. If you did this procedure as part of installation, no further verification is needed at this
time. Go back to Heading 3.7, ADJUSTMENTS AND CALIBRATION, and begin at step 2
under Verifying the Diluent and Lytic Reagent Dispense Timing.
2. If you did this procedure following replacement of a BSV, a CBC lytic reagent pump, or a
WBC diluent dispenser, verify calibration.
FACTORS.
3. If you did this procedure as part of the procedure for converting the instrument to
cyanide-free reagents, go back to Pre-Conversion Instrument Verification under
Heading 3.10, CONFIGURING THE MAXM ANALYZER FOR CYANIDE-FREE
REAGENTS, and begin at step 4.
CHECKS.
e. Run controls.
4.20-4 PN 4235961E
BLOOD/BUBBLE DETECTORS GAIN ADJUSTMENT 4
4.21 BLOOD/BUBBLE DETECTORS GAIN ADJUSTMENT
B DMM voltmeter
B Trim pot adjustment tool, PN 5415364
B Blood specimens
Procedure
1. Remove the left-side panel and, if the Sample Handler card is covered, remove the cover
to access the Sample Handler card. See Figure 2.1-5 for location of the card.
2. Ensure the instrument is ON.
3. Do a Startup cycle. Verify that no bubbles are left in the sample path from the pierce
needle to FF44 on the BSV module.
4. Adjust and record the voltage for the front blood/bubble detector:
a. Connect the DMM negative lead to TP5 on the Sample Handler card. See
Figure 4.21-1.
b. Connect the DMM positive lead to TP23 on the Sample Handler card. See
Figure 4.21-1.
c. Adjust potentiometer R64 (Figure 4.21-1) until the measured voltage on the DMM
equals the voltage labeled on the front detector cable ± the limits specified in
Table A.1-22, Blood/Bubble Detectors Tolerances.
d. Record the voltage for future reference.
Figure 4.21-1 Blood/Bubble Detector Test Points and Adjustments
TP5 (ground)
TP23 (front detector voltage)
R77 (rear detector adjustment)
TP19 (rear detector voltage)
R64 (front detector adjustment)
5961048D
PN 4235961E 4.21-1
BLOOD/BUBBLE DETECTORS GAIN ADJUSTMENT
5. Adjust and record the voltage for the rear blood/bubble detector:
a. Leave the DMM negative lead connected to TP5 on the Sample Handler card. See
Figure 4.21-1.
b. Remove the DMM positive lead from TP23 and connect it to TP19. See
Figure 4.21-1.
c. Adjust potentiometer R77 (Figure 4.21-1) until the measured voltage on the DMM
equals the voltage labeled on the rear detector cable ± the limits specified in
Table A.1-22, Blood/Bubble Detectors Tolerances.
d. Record the voltage for future reference.
IMPORTANT The circuitry on the Sample Handler card is set up for matched sets of blood detectors, and
may not work correctly with a nonmatched set. Never replace a single blood detector out of a pair. Always
replace both blood detectors with a factory matched set.
6. If voltages measured in steps 4 and 5 cannot be adjusted to match level labeled:

a. Prime the instrument. From the Main menu of the DMS, select Diluter Functions tt
Prime Reagents All.
b. Repeat steps 3 through 5.
c. If the condition still exists, replace the blood detectors.
Verification
1. Select Run Samples from the Sample Analysis menu in the Primary-mode.
2. Run a number of blood samples:
a. Ensure that the blood samples are positioned between the front and rear blood
detectors during BSV segmentation and that no aspiration errors occur.
b. Ensure the backwash appears good.
c. Measure the voltage at TP19 and TP23 (Figure 4.21-1) and ensure that within
2 minutes after the backwash the voltages return to the same value recorded in
steps 4 and 5 ± the limits specified in Table A.1-22, Blood/Bubble Detectors
Tolerances.
3. Install the Sample Handler card cover and the left-side panel.
4. If adjusting the blood/bubble detector gains as part of installation, no further verification
is needed at this time. Go back to Heading 3.5, INITIAL SYSTEM SETUP, and begin at
step 2 under Adjusting the Blood/Bubble Detector Gains.
4.21-2 PN 4235961E
BLOOD/BUBBLE DETECTORS GAIN ADJUSTMENT 4
CHECKS.
e. Run controls.
PN 4235961E 4.21-3
BLOOD/BUBBLE DETECTORS GAIN ADJUSTMENT
4.21-4 PN 4235961E
ELECTRONIC POWER SUPPLY REPLACEMENT 4
4.22 ELECTRONIC POWER SUPPLY REPLACEMENT
Removal
covers to electronic components.
2. Open the rear Analyzer module doors.
3. Disconnect P105, P115, P116, P112, P108, P107, P113, and P114 from their respective
connectors on the rear of the Electronic Power Supply. See Figure 4.22-1.
Note: P119 is not used.
Figure 4.22-1 Electronic Power Supply Connectors

P119
P105 (Not used) P115 P116 P112 GND P108 P107 P113 P114
J113
J107
1
6 4
1
J105
J108
12 10
J116
12 10
J114
J119
3 1
15
3 1
3
5961037D
4. Disconnect the ground wires.

5. Open the upper front cover.
6. Remove the Electronic Power Supply mounting bracket.
7. Pull out the Electronic Power Supply from the front of the instrument.
Installation
1. Inspect the new Electronic Power Supply and reconfigure it if necessary:
a. Remove the Electronic Power Supply cover.
b. Verify that all Electronic Power Supply Terminal card (TB) jumpers and
connections are configured for the correct input line voltage. Refer to Table A.3-3,
Electronic Power Supply Terminal Card Jumpers and Connections.
c. Reinstall the Electronic Power Supply cover.
PN 4235961E 4.22-1
ELECTRONIC POWER SUPPLY REPLACEMENT
CAUTION The opening for the Electronic Power Supply contains wiring harness connectors which could be
jammed behind the Electronic Power Supply. As you slide in the Electronic Power Supply, access the wiring
harness connectors from the rear of the Main Unit and move them as needed to avoid jamming.
2. At the front of the Main Unit, position the Electronic Power Supply on its guide rails,
and carefully slide the Electronic Power Supply into the opening.
3. Connect wiring harness plugs P105, P107, P108, P112, P113, P114, P115, and P116 to
their respective connectors on the Electronic Power Supply. (P119 is not connected.)
4. Connect the ground wire (green) to the top rear edge of the Electronic Power Supply
chassis.
Note: Push the Electronic Power Supply slightly forward to expose the ground E-Point.
5. Close the rear Analyzer module doors.
6. Install the Electronic Power Supply mounting bracket at the front of the instrument.
7. Close the upper front cover.
9. Check the voltages and adjust if necessary as directed under Heading 4.23, VOLTAGE
CHECKS AND ADJUSTMENTS.
4.22-2 PN 4235961E
VOLTAGE CHECKS AND ADJUSTMENTS 4
4.23 VOLTAGE CHECKS AND ADJUSTMENTS
This section includes two voltage check procedures.
r Use the System Voltage Checks to verify, and adjust if necessary, the system voltages:
t At instrument installation.
t After replacing the Electronic Power Supply.
t When troubleshooting system voltage error messages.
r Use the Laser On Current/LS Offset Voltage Check to verify the laser on current/LS
voltage offset is correct:
t At instrument installation.
t When troubleshooting light scatter problems.
System Voltage Checks
Using the DMS to Check System Voltages

1. Select Special Functions tt Diagnostics tt Operator Options tt System Test.
2. Press Ë to start the System Test.
3. Ensure the voltages are within the limits specified in Table A.1-23, System Voltage
Ranges.
4. If necessary, adjust the voltages as directed below in Using an External DMM to Check
and Adjust System Voltages.
5. Verify the instrument is working correctly. Go to System Voltage Checks Verification at
the end of this section.
Using an External DMM to Check and Adjust System Voltages

1. Open the upper front door to access the front of the Electronic Power Supply
(Figure 4.23-1).
2. Read the voltages at the test points (J120) and ensure that the voltages are within the
limits specified in Table A.1-23, System Voltage Ranges.
3. If the voltages are not within limits, verify the Electronic Power Supply is configured
correctly, and adjust the +5, +5.6, +12, +24, +6.3, +300, +15, or -15 Vdc as necessary.
covers to electronic components.
a. Power down the instrument and remove the Electronic Power Supply as directed
under Heading 4.22, ELECTRONIC POWER SUPPLY REPLACEMENT.
b. Remove the cover from the Electronic Power Supply.
c. Verify that all Electronic Power Supply Terminal card (TB) jumpers and
connections are configured for the correct input line voltage. Refer to Table A.3-3,
Electronic Power Supply Terminal Card Jumpers and Connections.
d. Insert a jumper between pins 5 and 6 of J108. See Figure 4.23-2.
PN 4235961E 4.23-1
VOLTAGE CHECKS AND ADJUSTMENTS
Figure 4.23-1 Electronic Power Supply, Front View

J113 J114 J107 J108 J112 J116 J115 J119 J105
VOLTAGE TEST
-15v
+5v +12v
+5.6v +6.3v
+15v GND
+24v
UNIT CONFIGURATION
100 120 220 240 CAUTION

VAC VAC VAC VAC ELECTRIC SHOCK
HAZARD. PANEL
TO BE OPENED
BY QUALIFIED
50/60 HZ SERVICE PER-
FRONT SECTION CENTER SECTION REAR SECTION ONEL ONLY.
J120 5961219D
Figure 4.23-2 Electronic Power Supply, Top View with Cover Removed
J108 J116 J112 J119
J107
DANGER J115
J114 J105
12 10
12
3
3
J119
J114
J116
J108
15
J113
10
1
1
1
J105
J115
6
3
6
3
J113
J112
13
1
J107
+6.3 Vdc R1
1
4
4
1
1
+300 Vdc R2
+15 Vdc
R27
-15 Vdc
R31
120V
+24 Vdc
R11
+12 Vdc
R11 +5.6 Vdc
R6
J120 +5 Vdc
R10
5961226D
4.23-2 PN 4235961E
VOLTAGE CHECKS AND ADJUSTMENTS 4
e. Power up the instrument. Refer to Power Up under Heading 4.1, GUIDELINES FOR
SERVICING THE MAXM ANALYZER.
.
WARNING Lethal voltages are present in the Electronic Power Supply. Use extreme caution when making
voltage adjustments.
f. Connect the DMM to the appropriate test points on the Power Supply modules to be
adjusted. Refer to Figure 4.23-2 and to Table A.3-4, Electronic Power Supply Test
Points and Adjustments).
Note: +300 Vdc must be turned ON to measure.
CAUTION Misadjusting the Power Supply potentiometers could damage electronic components or cause
the instrument to sense the voltages incorrectly. DO NOT adjust any potentiometer that is not listed in
Table A.3-4, Electronic Power Supply Test Points and Adjustments. When adjusting a Power Supply voltage,
adjust it to the value specified in Table A.3-4, Electronic Power Supply Test Points and Adjustments.
g. While observing the voltages at the test points, adjust the potentiometers as
required (Figure 4.23-2 and Table A.3-4, Electronic Power Supply Test Points and
Adjustments).
h. Power down the instrument as directed in Power Down/Power Up the System under
i. Remove the jumper from J108 (Figure 4.23-2).
j. Install the cover on the Electronic Power Supply.
k. Install the Electronic Power Supply in the Main Unit and power up the instrument.
Refer to Installation under Heading 4.22, ELECTRONIC POWER SUPPLY
REPLACEMENT.
4. Close the upper front door and verify the instrument is working correctly. Go to System
Voltage Checks Verification at the end of this section.
Laser On Current/LS Offset Voltage Check

B Dark Current Test Plug, PN 2121422 (for instruments with an LS Preamp 3 module)
B Service Disk
Procedure
Note: Laser on current readings are proportional to LS offset voltage readings.
1. Open the right-side door of the Main Unit to access the LS Preamp module.
WARNING The laser beam can cause eye damage if viewed either directly or indirectly from reflective
surfaces (such as a mirror or shiny metal surface). Ensure the laser cover is on and the front doors of the
Main Unit are closed while you do the Laser On Current/LS Offset Voltage Check.
2. Ensure the laser cover is on and the front doors of the Main Unit are closed.
3. If the MAXM analyzer has an LS Preamp 5 module, set up the DMM as follows:
a. Set the DMM to measure volts.
b. Connect the DMM to TP1 and TP3 on the LS Preamp 5 module.
PN 4235961E 4.23-3
VOLTAGE CHECKS AND ADJUSTMENTS
4. If the MAXM analyzer has an LS Preamp 3 module, set up the DMM as follows:
a. Disconnect the leads of the LS sensor from the LS Preamp 3 module.
b. Connect the Dark Current Test Plug to the LS sensor leads.
c. Connect the DMM to the Dark Current Test Plug.
d. Set the DMM to read µA.
6. Read the laser on current (LS Preamp 3 module) or LS offset voltage (LS Preamp 5
module) in the Diff Latex-Calibration mode.
a. From the Service Options menu, select Latex Checks tt Diff Latex Calibration.
ATTENTION: Take the LS offset reading before the DMS displays the message, To stop rerun,
press aspiration switch now, to avoid noise interference.
c. Cycle diluent and measure the laser on current or LS offset voltage while the
instrument is "counting."
d. Ensure the current/voltage reading in the Diff mode is within the limits specified in
Table A.1-19, LS Offset Voltage/Laser On Current Checks.
e. If you installed the Dark Current Test Plug, disconnect the LS sensor leads from the
Dark Current Test Plug and reconnect them to the LS Preamp 3 module.
7. If the MAXM analyzer has the Retic option, read the LS offset voltage in the Retic
Latex-Calibration mode.
a. From the Service Options menu, select Latex Checks tt Retic Latex Calibration.
c. Cycle diluent and measure the LS offset voltage while the instrument is "counting."
d. Ensure the voltage reading in the Retic mode is within the limits specified in
Table A.1-19, LS Offset Voltage/Laser On Current Checks.
8. If you measured the laser on current/LS offset voltage as part of installation, record the
results on the Installation Test Data Log Sheets as directed in Measuring the LS
Current/LS Offset Voltage, step 2, under Heading 3.7, ADJUSTMENTS AND
CALIBRATION.
System Voltage Checks Verification

Verify instrument performance:
1. On instruments without a pressure tank, check the 30 and 60 psi as directed under
2. On instruments with a BSV that uses air cylinders, do the BSV and BSV Housing
3. Do a Start Up or System Test.
4. Check reproducibility as instructed under Heading 4.5, REPRODUCIBILITY CHECKS.
5. Run controls.
4.23-4 PN 4235961E
PNEUMATIC POWER SUPPLY REPLACEMENT 4
4.24 PNEUMATIC POWER SUPPLY REPLACEMENT
Removal
2. At the rear of the instrument, remove the eight screws that secure the Pneumatic Power
Supply to the instrument. See Figure 4.24-1.
Figure 4.24-1 Pneumatic Supply Removal
Remove
screws
(8)
5961237D
3. Pull the Pneumatic Power Supply partially out from the rear of the instrument.
4. Disconnect the pressure and vacuum tubing from the instrument.
5. Disconnect J90 from the left front of the Pneumatic Power Supply (left/right orientation
from the front of the instrument).
6. Disconnect the ground wire for the Pneumatic Power Supply from the chassis.
7. Remove the Pneumatic Power Supply.
Setup of Pneumatic Power Supply Buck-Boost Transformer

1. Measure and record the ac line voltage at the socket designated for the instrument.
2. Configure the Pneumatic Power Supply Buck-Boost Transformer Terminal card to the
appropriate configuration for the line voltage measured. Refer to Table A.3-6, Pneumatic
Power Supply Buck-Boost Transformer Terminal Card Jumpers and Connections.
PN 4235961E 4.24-1
PNEUMATIC POWER SUPPLY REPLACEMENT
Installation
1. Slide the Pneumatic Power Supply partially into the instrument.
2. Connect the ground wire to the chassis.
3. Connect J90 to the Pneumatic Power Supply. Refer to Figure 4.24-1
4. Connect the pressure and vacuum lines to the instrument.
5. Slide the Pneumatic Power Supply fully into the instrument.
6. Install the eight screws that secure the Pneumatic Power Supply to the instrument.
8. Verify the pressure and vacuum settings are correct as directed under Heading 4.25,
PRESSURE/VACUUM ADJUSTMENT.
4.24-2 PN 4235961E
PRESSURE/VACUUM ADJUSTMENT 4
4.25 PRESSURE/VACUUM ADJUSTMENT
General
You can use a DMS or an external DMM to monitor and adjust pressure and vacuum settings.
r If you are using the DMS, use the pressure and vacuum readings on the DMS screen in
the following procedures.
r If you are using a DMM, use the voltage readings on the DMM in these procedures.
r Refer to Table A.2-6 to convert the pneumatic readings to Vdc readings and vice versa.
System Test (Pressure/Vacuum Verification)

1. Select Special Functions tt Diagnostics tt Operator Options tt System Test.
2. Press Ë to start the System Test.
3. Ensure the vacuum and pressure readings are within the limits specified in Table A.1-13,
Pressure and Vacuum Tolerances.
4. If the readings are not within limits, make the necessary adjustments as directed under:
r 60-psi Pressure Adjustment
r 30-psi Pressure Adjustment
r Sheath Pressure Adjustment
r Initial Sample Pressure Adjustment
r High Vacuum Verification
r Low Vacuum Adjustment
Pressure Adjustments
B A digital pressure meter, such as a Marsh Meter, PN 2906639 (For 60-psi pressure
adjustment)
60-psi Pressure Adjustment

1. Remove the left-side panel of the Main Unit and remove the Pneumatic Power Supply’s
side access panel to access the 60-psi regulator (pressure relief valve). See Figure 4.25-1
or Figure 4.25-2.
IMPORTANT The voltage potentiometers on the Pneumatic Monitor card are preset and if misadjusted
might cause the instrument to sense the pneumatic signals incorrectly. Do NOT adjust the voltage
potentiometers on the Pneumatic Monitor card.
2. If you are using a DMM:

a. Remove the cover from the Pneumatic Monitor card. For the location of the
Pneumatic Monitor card, refer to Figure 2.1-3.
b. Connect the DMM to TP200 and TP2 (ground). Refer to Figure A.2-7.
3. Unlock the 60-psi regulator:
a. If the regulator is configured as shown in Figure 4.25-1, pull up the locking collar.
b. If the regulator is configured as shown in Figure 4.25-2, pull up the knob.
PN 4235961E 4.25-1
PRESSURE/VACUUM ADJUSTMENT
Figure 4.25-1 60-psi Pressure Regulator, Old Configuration
Adjustment
knob
Locking
collar
5961040D
Figure 4.25-2 60-psi Pressure Regulator, New Configuration
Adjustment
knob
5961225D
4. Perform the System Test (Special Functions tt Diagnostics tt Operator Options tt System Test).
5. Turn the adjustment knob until the DMS reads 60 psi or the DMM reads 8.57 Vdc.
6. After adjustment, push down the locking collar (old configuration) or push down the
adjustment knob (new configuration) to lock the knob in position, preventing it from
turning.
4.25-2 PN 4235961E
7. Verify the compressor is supplying adequate pressure at peak load:
a. Disconnect the 60 psi line from the Pneumatic Monitor card and attach the line to a
Y- or T-fitting.
b. Attach 5 cm (2 in.) pieces of tubing to the remaining two legs of the Y- or T-fitting.
c. Connect one leg of the Y- or T-fitting to the Pneumatic Monitor card and the other
leg to the digital pressure meter.
d. Do a normal whole blood cycle and ensure that throughout the cycle the 60-psi
pressure never drops below the minimum acceptable peak load pressure specified in
Table A.1-13, Pressure and Vacuum Tolerances.
e. Disconnect the Y- or T-fitting and reconnect the 60-psi line to the Pneumatic
Monitor card.
8. Reinstall the side access panel on the Pneumatic Power Supply and the left-side panel on
the Main Unit.
9. If you are using a DMM, disconnect the DMM and install the cover on the Pneumatic
Monitor card.
a. On instruments with a BSV that uses air cylinders, do the BSV and BSV Housing
b. Do a Start Up or System Test.
c. Check reproducibility as instructed under Heading 4.5, REPRODUCIBILITY
CHECKS.
d. Run controls.
30-psi Pressure Adjustment
IMPORTANT The voltage potentiometers on the Pneumatic Monitor card are preset and if misadjusted
might cause the instrument to sense the pneumatic signals incorrectly. Do NOT adjust the voltage
potentiometers on the Pneumatic Monitor card.
1. Open the right-side door of the Main Unit to access the 30 psi regulator (pressure relief
valve), RG2. For the location of RG2, refer to Figure A.5-7.
a. Remove the cover from the Pneumatic Monitor card. For the location of the
Pneumatic Monitor card, refer to Figure 2.1-3.
b. Connect the DMM to TP100 (pin 7) and to TP2 (ground). Refer to Figure A.2-7.
4. Turn the 30-psi regulator adjustment knob until the DMS reads 30 psi or the DMM reads
8.57 Vdc.
5. Do a normal whole blood cycle and observe the RBC and WBC diluent dispensers.
Ensure each diluent dispenser reaches top-of-stroke, pushing the entire sample through
to the bath, before the center section of the BSV rotates back to the home position.
6. Close the right-side door.
PN 4235961E 4.25-3
Monitor card.
a. On instruments without a pressure tank, check the 60 psi as directed in 60-psi
Pressure Adjustment above.
CHECKS.
e. Run controls.
Sheath Pressure Adjustment

1. Open the right-side door of the Main Unit to access the sheath pressure regulator, RG3.
For the location of RG3, refer to Figure A.5-7.
a. Remove the cover from Pneumatic Monitor card. For the location of the Pneumatic
Monitor card, refer to Figure 2.1-3.
b. Connect a DMM to TP400 (pin 2) and to TP2 (ground). Refer to Figure A.2-7.
ATTENTION: The sheath-pressure regulator is a non-relieving regulator. To adjust pressure
down, turn the knob well below the desired pressure setting and bleed the line on the
regulated side. After bleeding the line, adjust the pressure up to the desired value.
4. Turn the sheath pressure regulator adjustment knob until the DMS reads 6.0 psi or the
DMM reads 4.286 Vdc.
Monitor card.
a. On instruments without a pressure tank, check the 30 and 60 psi as directed in
60-psi Pressure Adjustment and 30-psi Pressure Adjustment above.
CHECKS.
e. Run controls.
4.25-4 PN 4235961E
Initial Sample Pressure Adjustment
1. Open the right-side door of the Main Unit to access the sample pressure regulator, RG4.
For the location of RG4, refer to Figure A.5-7.
ATTENTION: The sample-pressure regulator is a non-relieving regulator. To adjust pressure
down, turn the knob well below the desired pressure setting and bleed the line on the
regulated side. After bleeding the line, adjust the pressure up to the desired value.
4. Turn the sample pressure regulator adjustment knob until the DMS reads 0.8 psi or the
DMM reads 5.33 Vdc.
Monitor card.
CHECKS.
e. Run controls.
8. Refer to Heading 4.10, VCS FLOW-RATE ADJUSTMENT for final sample pressure
adjustment.
Vacuum Adjustments
High Vacuum Verification

Note: The high vacuum system cannot be adjusted. This test is to verify that the Hi Vac
reading is at the proper value. High vacuum varies depending on the altitude at which the
instrument is located.

PN 4235961E 4.25-5
3. Verify the high vacuum reading on the DMS is within the limits specified in
Table A.1-13, Pressure and Vacuum Tolerances, or the reading on the DMM is within the
equivalent voltages specified on Table A.2-6.
Monitor card.
CHECKS.
e. Run controls.
Low Vacuum Adjustment

1. Open the right-side door of the Main Unit to access the low vacuum regulator, RG1. For
the location of RG1, refer to Figure A.5-7.
4. Turn the low vacuum regulator adjustment knob until the DMS reads 6.0 in. Hg. or the
DMM reads 5.0 Vdc.
Monitor card.
CHECKS.
e. Run controls.
4.25-6 PN 4235961E
SOLENOID VALVES INSPECTION AND REPLACEMENT 4
4.26 SOLENOID VALVES INSPECTION AND REPLACEMENT
B Service Disk
Solenoid Inspection
CAUTION Manually energizing LV70 without disconnecting the waste line to the waste container will
pressurize the container. Disconnect the waste line from the waste container before energizing LV70.
3. Use the arrow keys to select the desired solenoid.

4. Press Û to highlight the chosen solenoid.
5. Press Ê to energize the solenoid. Check the instrument for the actions listed in
Table 4.26-1, Solenoid Operations.
Note: The solenoid LED lights to indicate solenoid operation. The solenoid is energized
for 10 minutes.
6. If components associated with the solenoid do not function, troubleshoot the system.
Table 4.26-1 Solenoid Operations
Location Components
LV Reference Activated Normal State Operated State
1 Figure A.5-5 PV4 Provides a path for low vacuum to VC1, Closes low vacuum path. Opens 30 psi
VC10, and VC11. pressure path to VC1, VC10, and VC11.
2 Figure A.5-5 PV2, PV66, Closes path from RBC aperture to VC1. Opens path from RBC aperture to VC10;
PV67, PV68, applies low vacuum to VC10 and vent to
PV69 VC2 for sweep flow; and closes path
from VC10, VC11 to VC1.
3 Figure A.5-5 PV5, PV6 VL5 closes drain from RBC bath to VC1. VL5 opens drain from RBC bath to VC1.
VL6 closes drain from WBC bath to VL6 opens drain from WBC bath to VC1.
VC1.
4 Figure A.5-5 PV1, PV7 VL1 provides path to fill CBC lytic VL1 closes fill path for PM1 and PM2
reagent pumps (PM1 and PM2). and opens CBC lytic reagent path to
WBC and BSV.
VL7 provides path for vacuum to PM1 VL7 closes vacuum path to PM1 and
and PM2. PM2 and opens pressure path to
dispense CBC lytic reagent from pumps.
5 Figure A.5-5 PV3 Closes path from WBC aperture to VC1. Opens path from WBC aperture to VC1.
6 Figure A.5-5 PV8 Provides paths from RBC and WBC Applies vacuum to RBC and WBC baths.
baths to overflow cuvette. Opens path for bleach to RBC and WBC
baths.
7 Figure A.5-5 None Provides low pressure mixing bubble air
to WBC bath.
PN 4235961E 4.26-1
SOLENOID VALVES INSPECTION AND REPLACEMENT
Table 4.26-1 Solenoid Operations (Continued)
Location Components
8 Figure A.5-5 None Provides low pressure mixing bubble air
to RBC bath.
9 None LV9 is not the number of a solenoid; it is
the number of the software signal used
to control the cycle counter.
10 Figure A.5-7 PV62 Provides vacuum from VC7 to FD7,
cleaning agent optical sensor.
11 Figure A.5-7 PV63 Provides vacuum from VC7 to FD6,
diluent optical sensor.
12 Figure A.5-7 PV64 Provides vacuum from VC7 to FD5, CBC
lytic reagent optical sensor.
13 Figure A.5-7 PV61, PV65 Provides vacuum from VC7 to FD3 and
4, PAK LYSE and PAK PRESERVE
optical sensor.
14 Figure A.5-3 PV12, PV13 VL12 provides path for vacuum to air VL12 provides a path for 30-psi
pump PM3. pressure to PM3.
VL13 provides vent path to fill PM3. VL13 provides a path for air pressure
from PM3 to BSV.
15 Figure A.5-3 PV14 Opens path from BSV to Primary-mode Closes path from BSV to Primary-mode
aspiration pump. aspiration pump.
16 Figure A.5-1 Applies air pressure to BSV aspirator tip
Figure A.5-2 air cylinder, CL1 (old BSV
configuration), or to BSV actuator (new
BSV configuration) to rotate aspirator
tip to aspirate position.
17 Figure A.5-1 Applies air pressure to CL1 (old BSV
Figure A.5-2 configuration), or to BSV actuator (new
BSV configuration) to return aspirator
tip to home position.
18 Figure A.5-1 Applies air pressure to BSV segment air
Figure A.5-2 cylinder, CL2 (old BSV configuration),
or to BSV actuator (new BSV
configuration), to return center section
of BSV to aspirate position.
19 Figure A.5-1 Applies air pressure to air cylinder CL2
Figure A.5-2 (old BSV configuration), or BSV
actuator (new BSV configuration) to
rotate center section of BSV to segment
position.
20 Figure A.5-3 PV42 Vents probe clean cylinder, CL3. Routes 30-psi pressure to CL3 to
extend the probe rinse block, and
activates VL42 which opens the path for
pressurized diluent or cleaning agent to
the aspirator tip.
4.26-2 PN 4235961E
Location Components
21 Figure A.5-3 PV10 Opens path for backwash diluent to
waste.
22 Figure A.5-3 PV11 Opens path for bleach through sample
valve module.
23 Figure 2.8-1 None Retracts tube clamping cylinder, CL4. Extends CL4.
Note: Applicable to MAXM analyzer with Note: Applicable to MAXM analyzer with
Rotary Cap-Pierce module only. Rotary Cap-Pierce module only.
24 Figure 2.8-1 None Retracts tube pierce cylinder, CL5. Extends CL5.
Note: Applicable to MAXM analyzer with Note: Applicable to MAXM analyzer with
Rotary Cap-Pierce module only. Rotary Cap-Pierce module only.
25 Figure A.5-10 PV23, PV24 VL23 opens aspiration path from VL23 opens aspiration path from PM4
Figure A.5-11 Primary-mode aspiration pump, PM4, to needle.
to vent chamber, VC4.
VL24 opens vacuum path to PM4. VL24 opens path for 30 psi pressure to
PM4.
26 Figure A.5-10 PV25, PV26 VL25 opens path from Secondary-mode VL25 opens aspiration path from VL25
Figure A.5-11 aspiration pump, PM5, to vent chamber, to aspirator tip.
VC4.
VL26 opens vacuum path to PM5. VL26 opens path for 30-psi pressure to
PM5.
27 Figure A.5-10 PV27 VL27 opens Erythrolyse II reagent path VL27 opens Erythrolyse II reagent path
Figure A.5-11 to Erythrolyse II reagent pumps, PM6 from PM6 and PM7 to sheath tank and
and PM7. mixing chamber.
28 Figure A.5-10 PV22 Provides rinse path from needle to VC4. VL22 opens path from pressurized
Figure A.5-11 diluent for needle rinse.
29 Figure A.5-10 PV21 Opens path from needle bellows to Opens drain path from VC3 to VC7.
Figure A.5-11 waste, VC3.
30 Figure A.5-10 PV20 Opens vent path to VC4. Opens path from pierce needle to low
Figure A.5-11 vacuum, VC3, and from VC4 drain to
VC3.
31 Figure A.5-9 PV59 Opens path for sample pressure to diff Opens path from MC1 to diff waste,
mix chamber, MC1. VC7.
32 Figure A.5-9 PV52 Opens path from sheath tank, ST1, to
MC1.
33 Figure A.5-9 PV57 Opens drain path from MC1 to diff
waste chamber, VC7.
34 Figure A.5-9 PV51 Opens path from BSV to MC1.
35 Figure A.5-7 PV30 Opens vent path to WBC and RBC Opens path for 30 psi pressure to PM9
diluent pumps, PM9 and PM11. Opens and PM11. Opens diluent path from
path for diluent to PM9 and PM11. PM9 and PM11 to BSV.
PN 4235961E 4.26-3
Location Components
36 Figure A.5-7 PV31 Provides open path for low vac to low Maintains vacuum path to VC6. Opens
vac chamber, VC6. Provides open low drain path from VC6 to CBC module
vac path from VC6 to CBC module VC1. VC1.
37 Figure A.5-7 PV32 Provides vent path for bleach vacuum Closes VC5 vent path. Opens vacuum
chamber, VC5. path from VC5 to manual bleach probe.
Opens vacuum path from MF9 to VC5.
Opens path for pressurized diluent from
MF11, via VL46, to BSV.
38 Figure A.5-7 PV33 Opens bleach path from VC5 to mix
chamber module.
39 Figure A.5-7 PV34 Opens path from cleaning agent supply
to VC5.
40 Figure A.5-7 PV35 Opens waste path from probe rinse
block to VC7 via VL49.
41 Figure A.5-7 PV36 Opens waste path from BSV to VC7.
42 Figure A.5-7 PV37 Provides open vacuum path to PM10. Opens 30 psi pressure path to PM10.
Provides open cleaning agent path to Opens cleaning agent path to
PM10. Provides open pressurized pressurized diluent manifold, MF11.
diluent path from sheath tank to MF11.
43 Figure A.5-7 PV38 VL38 opens path from bleach probe to
BSV module.
43 Figure A.5-7 None Closes path between water trap and Purge solenoid. Opens path to dispense
waste. the water built up in the water trap to
waste. Software signal LV70 is used to
control this solenoid.
44 Figure A.5-7 PV28 Opens vacuum path to PM6 and PM7 Closes vacuum path and opens
pressure path to PM6 and PM7.
45 Figure A.5-7 PV40 Provides open drain path from VC7 to Opens vacuum path from vacuum
waste. manifold, MF13, to VC7 via FMT1.
46 Figure A.5-7 PV49 Provides open path for vacuum and Opens path for 30-psi pressure to PM8.
diluent to PM8. Provides open vacuum Opens diluent path for needle
path from probe wiper to VC7 via VL35. backwash.
47 Figure A.5-7 PV43 Opens drain path from low vacuum and
waste chamber, VC1.
48 Figure A.5-7 PV48 Provides open path from diluent supply Opens backwash path from cleaning
to PM11 via VL30. agent supply to PM11 via VL30.
49 Figure A.5-7 PV41 Provides open vent path for diff mixing Opens path from sample pressure
chamber, MC1, via VL59. regulator, RG4, to MC1 via VL59.
50 Figure A.5-7 PV44 Opens path for pressurized diluent to
flush upper half of flow cell
51 Figure A.5-7 PV45 Opens path for pressurized diluent
(sheath fluid) to flow cell.
4.26-4 PN 4235961E
Location Components
52 Figure A.5-7 PV46 Opens path for pressurized diluent to Opens drain path from flow cell to VC7.
the BSV. Opens vent path for FMT2.
53 Figure A.5-3 PV15 Opens path from BSV to Primary-mode Closes path from BSV to Primary-mode
aspiration pump. aspiration pump.
54 Figure A.5-9 PV54 Opens path for pressurized diluent to
flush lower half of flow cell.
55 Figure A.5-9 PV60 Opens drain path from flow cell to VC7.
56 Figure A.5-9 PV50 VL50 provides open StabiLyse reagent Opens StabiLyse reagent path from
path to StabiLyse reagent pump, PM12. PM12 to diff mixing chamber, MC1, via
VL53.
57 Figure A.5-9 PV55 Closes path from diff mixing chamber,
MC1, to flow cell.
58 Figure A.5-9 PV56 Opens drain path from flow cell to VC7.
59 Figure A.5-9 PV53, PV58 VL53 opens path from PM12 to MC1.
VL58 provides open path from vacuum VL58 opens path from 30-psi pressure
manifold, MF13, to StabiLyse reagent manifold, MF15, to PM12.
pump, PM12.
60 - Spare
64
65 None LV65 is not the number of a solenoid; it
is the number of the software signal
used to turn mix motor on.
used to turn RF on (300 Vdc).
67 Figure A.5-7 PV47 Provides open path from sheath tank to Opens path from diluent manifold,
pressurized diluent manifold, MF11. MF12, to sheath tank. Opens drain path
Provides open low pressure path from from sheath tank to VC7.
sheath regulator, RG3, to sheath tank.
68 Figure A.5-7 Bleeds the compressor.
69* None *LV69 is not the number of a solenoid;
it is the number of one of the software
signals used to turn the compressor on.
used to energize the water trap
solenoid, LV43.
71* None *LV71 is not the number of a solenoid;
it is the number of one of the software
signals used to turn the compressor on.
81 Figure 2.9-3 CL9 Not activated. Extends CL9 to push the specimen tube
back into the cassette.
PN 4235961E 4.26-5
Location Components
82 Figure 2.9-3 CL7 (with Retracts the tube ram cylinder, CL7, in Extends CL6 to pierce the specimen
LV83), CL6 conjunction with LV83, and retracts the tube, and works in conjunction with
needle drive cylinder, CL6. LV83 to extend CL7 to push the
Note: LV82 vents port 1 of CL7 and CL6 specimen tube out of the cassette.
and applies pressure to port 2 of CL7 Note: LV82 vents port 2 of CL6 and
and CL6 to retract both cylinders. applies pressure to port 1 of CL6 to
LV83 vents port 1 of CL7 to allow extend CL6 and vents port 2 of CL7 to
retraction of CL7. allow extension of CL7.
LV83 applies pressure to port 1 of CL7
to extend CL7.
83 Figure 2.9-3 CL7 (with Retracts the tube ram cylinder, CL7, in Extends CL7 in conjunction with LV82
LV82) conjunction with LV82. to push the specimen tube out of the
For details, see LV82 above. cassette.
For details, see LV82 above.
84 Figure 2.9-3 CL8 Retracts the bed-lock cylinder, CL8, Extends CL8 to lock the rocker bed in
allowing the rocker bed to rock. position.
* Solenoid software signals 69 and 71 must be activated simultaneously to turn the compressor on.
Solenoid Replacement
Removal
CAUTION The solenoid wires are fragile and break easily. Handle the solenoid wires carefully when
disconnecting the wires from the solenoid.
2. Disconnect the solenoid wires from the solenoid.

3. Remove the two screws that secure the solenoid to its manifold.
4. Remove the tubing from the solenoid.
5. Remove the defective solenoid from the manifold.
Installation
1. Connect the wires and tubing to the new solenoid.
2. Install the solenoid on its manifold with two mounting screws.
3. Power up the instrument and go to Verification.
4.26-6 PN 4235961E
Verification
1. Verify correct solenoid operation as directed under the previous heading, Solenoid
Inspection, steps 1 through 5.
CHECKS.
e. Run controls.
PN 4235961E 4.26-7
4.26-8 PN 4235961E
HEMOGLOBIN PREAMP MODULE REPLACEMENT 4
4.27 HEMOGLOBIN PREAMP MODULE REPLACEMENT
Purpose
Use this procedure to replace the Hgb Preamp module as a complete assembly.
Removal
2. Open the upper front door and the right-side panel of the Main Unit.
3. In the right-side of the Main Unit, disconnect the Hgb Preamp module cable, P26,
from J26.
4. Remove the nuts and associated washers from the rear of the two mounting screws that
secure the Hgb Preamp module to the panel. See Figure 4.27-1 for the location of the
Hgb Preamp module mounting screws.
Figure 4.27-1 Hgb Preamp Module
Optic
lamps
Hgb Preamp
module
Hgb lamp mounting screws
screw
Thumbscrews Hgb Preamp

module
Optic lamp Hgb lamp Optic lamp

connector connector connector 5961043C
5. While supporting the Hgb Preamp module, remove the mounting screws.
6. Remove the Hgb Preamp module, and pull cable P26 through the mounting hole.
Installation
1. Feed the cable on the new Hgb Preamp module from the front of the panel, through the
mounting hole, to the rear of the panel.
2. Position the new Hgb Preamp module on the front of the panel, and reinstall the
mounting screws and associated washers and nuts.
PN 4235961E 4.27-1
HEMOGLOBIN PREAMP MODULE REPLACEMENT
3. Connect P26 to J26.

4. Close the front and side panel doors.
6. Allow the instrument to warm up for 30 minutes. Then adjust Hgb-blank as instructed
below under Hemoglobin Lamp Adjustment
Hemoglobin Lamp Adjustment

1. From the Main menu, select Special Functions tt Diagnostics tt Operator Options tt HGB Lamp
Adjust.
2. If Hgb Lamp Adjustment: OK is displayed, go to Verification. Otherwise, press ÈÛ to
repeat the Hgb adjustment.
Note: It may require up to three attempts to adjust the Hgb lamp.
Verification
5. Run controls.
4.27-2 PN 4235961E
APERTURE MODULE AND BATH ASSEMBLY 4
4.28 APERTURE MODULE AND BATH ASSEMBLY
Removal
1. Drain the aperture baths.
3. Open the upper front door of the Main Unit.
4. Remove the two screws securing the electromagnetic shield to the CBC module and lift
off the shield.
WARNING The optic lamp is hot. You can get burned if you handle the lamp while it is hot. Let the optic
lamp cool before you remove it.
5. Unscrew the thumbscrew that holds the optic lamp (Figure 4.28-1) to the bath and
remove the lamp.
6. Disconnect the external electrode.
7. If removing the WBC bath, remove the Hgb Preamp module. Refer to Heading 4.27,
HEMOGLOBIN PREAMP MODULE REPLACEMENT.
8. Disconnect the bath from the bath holder by removing the retaining screw on the bottom
of the bath (Figure 4.28-1).
Figure 4.28-1 Aperture Module and Bath Assembly
Aperture
module Electrode
Aperture
Retaining
screws
Bath
Aperture
block
Optic lamp
O-rings
Hose
locking
screw
Electrode
Thumbscrew
Retaining screw
Optic lamp
jack
5961227D
PN 4235961E 4.28-1
APERTURE MODULE AND BATH ASSEMBLY
9. Separate the bath from the aperture housing with a gently rocking motion.
10. To remove the aperture block from the Aperture module.
a. Loosen the fitting at the base of the Aperture module (Figure 4.28-1).
b. Pull out the aperture block. Keep your fingers away from the aperture disk.
CAUTION Lubricant could damage the O-rings. Do not use any lubricant on the O-rings. Wet the O-rings
with distilled water during reassembly.
11. If replacing the aperture block:

a. Install new O-rings on the aperture block.
b. Install the new aperture block in the aperture module.
c. Go to Installation.
12. Inspect the aperture block O-rings and the bath mount O-ring, and replace if necessary.
13. To remove the Aperture module and bath assembly:
a. Label and remove the tubing from the bath.
b. Remove the tubing from the fittings on the Aperture module.
c. Remove the retaining screws securing the Aperture module (Figure 4.28-1).
d. Disconnect the signal cable from the Preamp card (Figure 4.28-1).
e. Disconnect ground cable J25 (RBC) or J24 (WBC).
f. Push the pins out of the connector.
g. Cut any tie wraps holding the signal cables together.
h. Pull the Aperture module forward.
14. Remove the objective lens from the rear of the module, and install it on the new module.
Installation
CAUTION Lubricant could damage the O-rings. Do not use any lubricant on the O-rings. Wet the O-rings
with distilled water during reassembly.
1. Reassemble and install the components in the reverse order of removing them. Ensure
that the junction points are free of salt deposits and that all the O-rings are in good
condition.
2. If you removed the Hemoglobin Preamp module, reinstall it. Refer to Installation under
Heading 4.27, HEMOGLOBIN PREAMP MODULE REPLACEMENT.
3. Reinstall the electromagnetic shield.
4. Close the front door and power up the instrument.
4.28-2 PN 4235961E
APERTURE MODULE AND BATH ASSEMBLY 4
Verification
1. If you replaced the aperture block:
a. Do the CBC Latex Calibration and Verification procedure as directed under
b. Do Heading 4.7, INITIAL ADJUSTMENT OF PRIMARY-MODE CALIBRATION
FACTORS.
c. Advise the customer that you have changed the calibration and that they need to
CHECKS.
e. Run controls.
PN 4235961E 4.28-3
APERTURE MODULE AND BATH ASSEMBLY
4.28-4 PN 4235961E
BSV AND BSV HOUSING/ACTUATOR DISASSEMBLY/REPLACEMENT 4
4.29 BSV AND BSV HOUSING/ACTUATOR DISASSEMBLY/REPLACEMENT
Purpose
Currently two different BSV assemblies are in use in the field; one uses air cylinders to rotate
the BSV (has BSV housing), the other uses an actuator. Use the procedures in this section for:
r BSV Disassembly/Replacement
r BSV Housing Replacement
r BSV Actuator Replacement
r BSV Alignment
r BSV and BSV Housing Verification
BSV Disassembly/Replacement
Purpose
IMPORTANT The BSV sections are tested as a set to ensure alignment of the ports. Mixing sections from
different sets could reduce control recovery and instrument performance. Replace all three sections of the
BSV as a complete assembly.
Use this procedure to disassemble and remove a BSV from either BSV assembly.
B Paper towels
B Lint-free tissues
BSV Removal
1. Open the upper front door of the Main Unit to access the BSV.
.
Figure 4.29-1 Retaining the Probe-Slide 2. At the DMS, select Special Functions tt
Mechanism Diagnostics tt Operator Options tt BSV
Tests tt BSV Removal.
3. Press Û to initiate the function.
4. Power down the instrument as directed
in Power Down/Power Up the System
under Heading 4.1, GUIDELINES FOR
Place 5. Fully extend the rinse block.
screwdriver
here 6. Insert a screwdriver in the hole behind
Sample
the sample bar to keep the probe-slide
bar mechanism out of the way. See
Figure 4.29-1.
5961215D SIDE VIEW
PN 4235961E 4.29-1
BSV AND BSV HOUSING/ACTUATOR DISASSEMBLY/REPLACEMENT
Figure 4.29-2 Removing the Rinse Block Screw 7. Remove the rinse block screw and
and Washer washer:
a. At the left side of the rinse block
(Figure 4.29-2), remove the
retaining screw and nylon washer.
b. Set the hardware aside.
c. Slide the rinse block off the end of
the aspirator tip.
8. Move the blood detector out of the way:
a. Remove the thumbscrew that holds
the bracket.
b. Position the blood detector over to
5934138C
the left, out of the way. Be careful
not to kink the tubing.
Figure 4.29-3 Moving the Air Cylinder 9. If the BSV assembly uses air cylinders, go
to step 10. If the BSV assembly uses an
actuator, skip to step 11.
10. Move the air cylinder out of the way. See
Figure 4.29-3.
a. Lift up the sleeve, and at the same
time, press the inside shaft down
with the needle-nosed pliers until
you see the ball.
b. Push the shaft back to free the ball.
c. Use your fingers to push the air
cylinder shaft up inside.
5934139C
IMPORTANT Damage to this aspirator tubing could cause partial aspirations. DO NOT bend, kink, crimp, or
crush the tubing when disassembling the BSV.
11. Disassemble the BSV:

a. Spread several layers of paper towels under the BSV.
b. Unscrew the knob on the BSV shaft. Remove the knob.
CAUTION The end of the shaft could scratch the inside surfaces of the BSV sections. As you remove the
BSV sections, slide them off carefully, ensuring that the inside surfaces do not contact the end of the shaft.
c. Use your left hand to very carefully slide the left section of the BSV off the shaft; let
it hang from its tubing.
4.29-2 PN 4235961E
CAUTION Pulling on the aspirator tip or on the metal loop of the BSV could damage the BSV. As you
remove and separate the right and center sections of the BSV, avoid pulling on the aspirator tip and the
metal loop.
d. Slide the center and right sections off together.

Note: Before you can get the BSV sections off, you may have to slightly rotate or
wiggle the sections to align the grooves in the center shaft.
e. Separate the center and right sections and let them hang from their tubing. DO NOT
remove the arm or bushing from the actuator shaft.
12. If you are replacing the BSV, remove the tubing from the BSV ports.
BSV Installation
1. Assemble the BSV:
a. If you are replacing the BSV, connect the tubing to the BSV ports.
b. Align the right section of the BSV. Line up the notch with the guide post.
c. Push the right section all the way to the right on the mounting and guide posts. Be
sure that none of the tubing is pinched.
d. Align the center section of the BSV. Line up the key on the center section with the
keyway on the guide post and the pin with the groove in the shaft.
e. Push the center section back to its position on the mounting post.
f. Line up the notch on the left section of the BSV with the guide post and, on
instruments with a BSV assembly that uses an actuator, line up the ball stud with the
left section bracket. Put the aspirator tip inside the sample bar.
g. Push the left section back to its position on the mounting post.
h. Screw the knob on until it does not turn any more; this takes many turns.
i. Use lint-free tissues to dry off all wet portions, particularly around the BSV.
Figure 4.29-4 Aligning the Air Cylinder 2. If the BSV assembly uses air cylinders, go
to step 3. If the BSV assembly uses an
actuator, skip to step 4.
3. Reassemble the air cylinder junction:
a. Position the aspirator tip straight up
and down.
b. Pull down the air cylinder shaft and
align the end:
1) Line up the slot in the sleeve
with the hole in the shaft.
2) Position this behind the ball.
3) Raise the sleeve with your
fingers until you see the entire
hole (Figure 4.29-4).
5934153C c. Place the ball in the hole in the
sleeve. Let the spring sleeve down
to encase the ball.
PN 4235961E 4.29-3
Figure 4.29-5 Replacing the Rinse Block 4. Screw the blood-detector bracket back
on firmly.
5. Reinstall the rinse block (Figure 4.29-5):
a. Slide the rinse block back onto the
aspirator tip.
b. Install the nylon washer and
retaining screw. Make the screw
finger tight, then tighten it 1/8 turn
more with a screwdriver. Do not
over tighten.
6. Remove the screwdriver you used to
immobilize the area.
7. Verify that all tubing:
r Is firmly attached
5934151C
r Goes under the shaft and guide post
r Is not twisted or pinched.
9. Verify the BSV assembly is working correctly:

r If you replaced the BSV and the BSV assembly uses air cylinders, go to the BSV
Alignment procedure.
r If you did not replace the BSV or if the BSV assembly has an actuator instead of air
cylinders, go to the BSV Disassembly/Replacement Verification below.
BSV Disassembly/Replacement Verification

1. From the Main menu, select Diluter Functions tt Start Up.
2. Press Û to initiate the Startup cycle.
3. Check the BSV during the Startup cycle and verify:
r The BSV is not leaking.
r The BSV sections rotate smoothly.
r The rinse block moves smoothly.
4. Close the upper front door.
Verification procedure as directed under Heading 4.1, GUIDELINES FOR
CHECKS.
e. Run controls.
4.29-4 PN 4235961E
BSV Housing Replacement
Purpose
If the BSV housing is corroded or malfunctions, use this procedure to replace the BSV housing
subassembly independently from the BSV and fit it with the BSV from the defective housing.
BSV Housing Removal

1. Remove the BSV. See BSV Disassembly/Replacement above.
2. Disconnect the BSV center section drive air cylinder from the BSV housing assembly.
3. Remove the two screws that hold the BSV center section drive arm to the BSV housing,
and remove the drive arm.
4. Remove the three socket-head screws and associated washers (one locking, one flat and
one black), that secure the BSV housing assembly to the mounting bracket.
5. Pull the BSV housing assembly from the mounting bracket.
BSV Housing Installation

1. Make sure a nylon spacer is in position on the BSV housing, and slide the housing into
the mounting bracket.
2. Secure the BSV assembly in the bracket with three socket-head screws and associated
washers (one locking, one flat and one black).
3. Install the BSV center section drive arm to the BSV housing.
4. Connect the BSV center section drive air cylinder to the BSV housing assembly.
5. Moisten the mating surfaces of the BSV sections with distilled water to form a wet seal.
6. Reinstall the BSV on the instrument. See BSV Installation under BSV
Disassembly/Replacement above.
8. Select BSV Tests tt Cycle BSV and check the BSV for free rotation.
9. Verify the BSV is correctly aligned. Go to the BSV Alignment procedure.
PN 4235961E 4.29-5
BSV Actuator Replacement

Purpose
Currently two configurations of the BSV actuator (configuration A and configuration B) are
in use. Use this procedure to replace either configuration.
B Four 0.093 i.d. fittings, PN 6232277
B Four O-rings, PN 2523062
CAUTION In configuration A of the BSV actuator assembly (Figure 4.29-6):

r The Allen screw is factory installed to a predetermined torque specification. Random adjustments can
cause incomplete rotation of the BSV sections and leaking between the sections.
r The cylinder seals are tested for pressure leaks at the factory. Removing the seals can cause pressure
leaks, compromising the operation of the BSV.
When replacing this configuration of the BSV actuator, do not adjust the Allen screw or remove the
cylinder seals.
Figure 4.29-6 BSV Actuator (Configuration A) Allen Screw and Cylinder Seals
Do not
remove Do not
adjust
7535022B
BSV Actuator Removal

1. Remove the BSV. See BSV Disassembly/Replacement above.
2. Remove the shaft bushing from the shaft of the actuator. For configuration A of the BSV
actuator, see Figure 4.29-7. For configuration B, see Figure 4.29-8.
3. Remove the left section segmenting bracket from the shaft of the actuator. For bracket
location, see Figure 4.29-7 (configuration A) or Figure 4.29-8 (configuration B).
4. Label the four tubings connected to the top of the actuator and disconnect the tubings
from the actuator. For tubing identification, see Figure 4.29-7 (configuration A) or
Figure 4.29-8 (configuration B).
5. Remove the three nuts (Figure 4.29-7) or screws (Figure 4.29-8) securing the alignment
bar to the actuator and remove the alignment bar.
6. If the BSV actuator is configuration B (Figure 4.29-8), remove the three centering
bushings from the BSV module.
7. Remove the actuator.
8. Return the actuator, the knob, the left section segmenting bracket, the shaft bushing
(configuration B only) and the centering bushings (configuration B only) for repair.
4.29-6 PN 4235961E
Figure 4.29-7 BSV Actuator Removal, Configuration A
LV17 LV18
BSV mounting
LV16 LV19
bracket
Left section
segmenting Alignment
bracket bar
Shaft Alignment bar

bushing install position 7535023A
Figure 4.29-8 BSV Actuator Removal,

g Configuration B
LV17 LV18
BSV mounting LV16 LV19

bracket
Centering
Left section Alignment bushings
segmenting bar (3)
bracket
Knob
Shaft Alignment bar

bushing install position 7535046A
BSV Actuator Installation

1. Remove the four plastic screws from the new BSV actuator assembly and replace each
with an O-ring and a 0.093 i.d. fitting.
2. Remove the knob and the shaft bushing (configuration B only) from the new BSV
actuator assembly.
PN 4235961E 4.29-7
3. Note the position of the left section segmenting bracket and the alignment bar on the
new BSV actuator assembly and then remove the following from the assembly:
r Left section segmenting bracket
r Alignment bar
r Three screws (configuration B only)
r Three centering bushings (configuration B only)
ATTENTION: While installing the shaft of the new BSV actuator assembly, hold the BSV tubing
so that it will be located on the front side of the BSV shaft (the side closest to you and furthest
away from the left section segmenting bracket and the BSV module panel).
4. Position the new BSV actuator assembly in the BSV module:
a. Insert the shaft of the new BSV actuator assembly through the large hole in the BSV
mounting bracket on the BSV module. Refer to Figure 4.29-7 (configuration A) or
b. On configuration A of the BSV actuator assembly (Figure 4.29-7), align the three
bolts extending from the BSV actuator assembly with the existing holes in the BSV
module bracket.
c. On configuration B of the BSV actuator assembly (Figure 4.29-8):
1) Align the three holes in the new actuator assembly with the three holes in the
BSV module.
2) Insert the three centering bushings in the holes in the BSV module.
d. Ensure the BSV tubing is located in front of the BSV shaft.
5. Slide the alignment bar onto the actuator shaft with the bar between the 8 and 9 o’clock
positions as shown in Figure 4.29-7 (configuration A) or Figure 4.29-8
(configuration B), and secure the alignment bar with the three nuts (configuration A) or
three screws (configuration B) provided with the new actuator assembly.
6. Ensure the cutouts in the BSV sections are free of crystallized diluent and cleaning agent.
7. Install the left section segmenting bracket supplied with the new BSV actuator assembly.
ATTENTION: On configuration A of the BSV actuator assembly (Figure 4.29-7), the opening of
the shaft bushing that faces the actuator and rests on the shoulders of the actuator shaft is
larger. If the orientation of this bushing is reversed, the bushing will not fit properly. On
configuration B the shaft bushing does not have a specific orientation.
8. With the larger diameter opening of the shaft bushing facing the actuator
(configuration A), slide the bushing onto the shaft and push it snug against the bracket.
9. Connect the four tubings labeled and removed in step 4 under BSV Actuator Removal, to
the top of the actuator. For tubing identification, see Figure 4.29-7 (configuration A) or
10. Reinstall the BSV on the instrument. See BSV Installation under BSV
Disassembly/Replacement above.
11. Verify the aspiration pump volume is correct as directed under Heading 4.17,
ASPIRATION PUMP VOLUME ADJUSTMENT.
4.29-8 PN 4235961E
BSV Alignment
ATTENTION: You do not need to do this BSV Alignment procedure on a BSV assembly that uses
an actuator instead of air cylinders to rotate the BSV sections.
1. Loosen and remove manifold MF2.
2. Loosen the three Allen screws that secure the BSV housing and the two Phillips-head
screws that secure the BSV shaft’s rear bushing. See Figure 4.29-9.
Figure 4.29-9 Aligning the BSV Housing

BSV
stiffener brackets
A A
MF2 20 21 22
Housing HOUSING
A B
BSV
5961203C
3. Remove the front BSV stiffener bracket (Figure 4.29-9) by removing the two
holding screws.
4. Inspect the cutouts of the BSV sections and the silver alignment bar to ensure that they
are free of any buildup.
5. Manually rotate the left section of the BSV while observing the air cylinder sleeve and the
ball stud (Figure 4.29-4). The sleeve should not come in contact with the narrow portion
of the ball stud. If it does, replace the air cylinder or BSV to satisfy this requirement.
6. Rotate the center and left sections of the BSV in both directions, to the silver alignment
bar. The movement should feel smooth. If it does not, replace the air cylinder or find the
cause of the restriction and correct it.
7. Disconnect each air cylinder and manually segment the BSV. Manually extend the
associated air cylinder to make sure that the cylinder is not at its extreme end of travel. If
the cylinder is at its extreme end of travel, replace the cylinder.
PN 4235961E 4.29-9
8. Reconnect all the air cylinders.

9. Cycle the BSV to seat the BSV sections.
a. Select Special Functions tt Diagnostics tt Operator Options tt BSV Test tt Cycle BSV.
b. Press Û.
10. After the instrument completes the cycle, gently push the BSV left and center sections
clockwise with respect to the BSV knob, to remove any gap between the BSV and the
silver alignment bar.
11. Tighten the three Allen screws (Figure 4.29-9).
12. Cycle the BSV again and verify that the left and center BSV sections rotate freely and
bank properly on the silver alignment bar.
13. Turn the two Phillip-head screws until they contact the plate (Figure 4.29-9).
14. Tighten the two Phillips-head screws an additional 1/8 of a turn.
Note: If the screws are too tight, the shaft does not move freely.
15. Reinstall the front BSV stiffener bracket(Figure 4.29-9):
a. Install and tighten the bottom screw.
b. Install and tighten the top screw.
Note: At this point you should be able to remove the stiffener brackets without using
force. If you have to use force, find the source of pressure on the mounts for the stiffener
brackets and correct the problem.
16. Reinstall manifold MF2.
17. Cycle the instrument and verify that the BSV moves freely, banks properly, and has
no leaks.
18. From the DMS, select Diluter Functions tt Start Up.
19. Press Û to initiate the Startup cycle.
20. Check the BSV during startup and verify:
r The BSV is not leaking.
r The BSV sections rotate smoothly.
r The rinse block moves smoothly.
21. Close the upper front door.
IMPORTANT Because the BSV affects the flow of blood to the aspiration pump and diluent to the aperture
baths, and because the BSV measures the blood used for the dilutions, changing a BSV can affect the
instrument results. Always check the volume pulled by the aspiration pump, the diluent delivery timing, and
the instrument calibration after replacing a BSV.
22. Verify the aspiration pump volume is correct as directed under Heading 4.17,
ASPIRATION PUMP VOLUME ADJUSTMENT.
4.29-10 PN 4235961E
BSV and BSV Housing Verification
ATTENTION: You do not need to do this BSV and BSV Housing Verification procedure on a BSV
assembly that uses an actuator instead of air cylinders to rotate the BSV sections.
Purpose
On instruments using the BSV housing, do this procedure as part of system verification at the
end of each service call.
B Several specimen tubes of normal whole blood
B A specimen tube of clean diluent
Procedure
1. Visually check the BSV and housing assembly. Clean off any debris on the alignment bar
and BSV cutouts.
2. Manually rotate the center and front sections of the BSV in both directions to the
alignment bar.
3. Cycle the instrument in both the Primary and Secondary modes and verify that the BSV
cutouts contact the alignment bar in both directions of travel.
4. Loosen the BSV knob. It should take at least three turns of the knob to release the spring
tension. If the knob separates from the front section of the BSV immediately, the BSV
shaft is binding. Determine the cause and take corrective action.
5. Tighten the BSV knob.
6. Ensure all the BSV tubing is correctly connected to the BSV.
7. Do a Startup cycle and verify the BSV and housing assembly are not leaking.
8. Run several bloods in the Primary mode with the blood/bubble detectors enabled
and verify:
a. During aspiration the blood segment is free of air gaps.
b. During the first pull, the blood segment is pulled to within 2.54 cm (1 in.) of the
front blood/bubble detector, BD1, but does not enter BD1. See Figure 4.29-10.
Figure 4.29-10 Aspiration First Pull (Aspiration Pump)
Blood FF44
Air
Diluent
2 1 3 12
12 2 1
BD1 BD2
Ensure leading edge

BSV
is within 2.54 cm (1 in.)
of BD1-2 but does not
enter BD1
5961288D
PN 4235961E 4.29-11
c. During the second pull, the trailing edge of the blood segment does not enter BD1.
See Figure 4.29-11.
Figure 4.29-11 Aspiration Second Pull (Vacuum)
Blood FF44
Air BSV
2 1 3 12 2 1
Ensure trailing edge BD1 BD2
does not enter BD1
5961289D
d. No aspiration error messages are generated.

9. Run a sample of clean diluent in the Primary mode with the blood/bubble detectors
enabled and verify an aspiration error message is generated.
4.29-12 PN 4235961E
CAROUSEL MOTOR REPLACEMENT 4
4.30 CAROUSEL MOTOR REPLACEMENT
Removal
2. Open the lower front door and remove the front panel.
3. Disconnect J1 from the Rotary Cap Pierce Junction II card.
4. Remove the motor wires from J1, pins 37, 38, 39, and 40.
5. Loosen the upper setscrew on the upper end of the carousel motor coupling one-half
turn. See Figure 4.30-1.
Figure 4.30-1 Carousel Motor and Carousel Assembly

MOUNTING
SCREWS (4)
MOTOR
U
CA
SETSCREW
CA
UT
OI
N
L O
C K
5961045E
6. Remove the four mounting screws that hold the tube guides, nut plates, and motor to the
mounting bracket. See Figure 4.30-1.
7. Remove the motor and spacers.
PN 4235961E 4.30-1
CAROUSEL MOTOR REPLACEMENT
Installation
CAUTION Over-torquing the mounting screws can damage the spacers and prevent the motor from
"floating" in its mounting holes. When installing the mounting screws, be careful not to over-tighten them.
1. Install the motor (with the flat side of the motor shaft toward the coupling setscrew),
spacers, nut plates, and screws onto the mounting bracket. Refer to Figure 4.30-1.
Tighten the screws to 6 in. lb of torque.
2. Tighten the setscrew that holds motor shaft to coupling (Figure 4.30-1).
3. Install the wires from the new motor to J1:
Attach: To:
Wire 6 Pin 38
Wire 7 Pin 39
Wire 8 Pin 40
Wire 9 Pin 37
4. Reconnect J1 to the Rotary Cap Pierce Junction II card.

5. Reinstall the front panel and close the lower front door.
Verification
5. Run controls.
4.30-2 PN 4235961E
CODE WHEEL ALIGNMENT 4
4.31 CODE WHEEL ALIGNMENT
B Three empty specimen tubes
Procedure
1. Turn off the compressor:
a. From the Main menu, select Special Functions tt Diagnostics tt Operator Diagnostics tt
Fluidics Tests tt Compressor On/Off.
b. Follow the directions on the screen to turn the compressor OFF. Allow time for the
compressor to shut off.
2. Press È as often as necessary to return the DMS to the Main menu. (This prevents the
instrument from accidentally cycling while working in the cap-pierce carousel area.)
Input: Channel 1
Vertical: 1.0 V/div.
Horizontal: 20 ms/div.
Synch: Auto/normal
Probe: X1
4. Remove the left-side panel of the Main Unit to access the Sample Handler card.
5. Locate circuit chip U35 on the left side of the Sample Handler card, about half-way down
from the top of the card. Refer to Figure A.2-12 for the Sample Handler II card.
CAUTION Touching the oscilloscope lead across any pins on U35 could cause a short circuit. Be careful
when connecting the oscilloscope lead to pin 5 on U35.
6. Connect oscilloscope channel 1 to pin 5 (normal tube size) on U35 of the Sample
Handler card.
7. Verify that the normal size tube holder is in home position, approximately centered with
tube slot in instrument door.
8. Loosen the code wheel nut until the code wheel can be manually rotated. See
Figure 4.31-1.
CAUTION Forcing the needle tip against the carousel could damage the needle. If the needle does not pass
easily through the 0.076-in. alignment hole, verify proper carousel positioning.
9. Ensure that the normal size tube holder is in the proper position (as in step 7 above), and
push the needle up carefully and slowly until it passes through the 0.076-in. alignment
hole, located 180 degrees away from the normal size tube position.
PN 4235961E 4.31-1
CODE WHEEL ALIGNMENT
10. Hold the needle in position. Find the trailing edge of the code wheel (marked H) on the
side of the larger lobe (Figure 4.31-1).
a. Rotate the code wheel slowly clockwise (with respect to looking up at the code
wheel) until sensor U3 senses light and the oscilloscope signal goes low.
b. Maintain the position of the code wheel, and tighten the code wheel nut to about
4 in. lb.
Figure 4.31-1 Code Wheel
Code Normal
wheel position Code wheel
sensors sensor nut
Oversized
Pierce position
position Detail of sensor
slot code wheel
Code
wheel
5961046E
11. Retract the needle from the alignment hole.

12. Rotate the carousel clockwise (with respect to looking up from the code wheel toward
the stepper motor) until the signal goes high.
13. Continue rotating, stopping immediately when the signal goes low. The carousel is now
in the pierce position for normal size tubes.
14. Carefully extend the needle through the 0.188-in. diameter hole in the carousel.
15. Ensure that the needle does not rub against the edge of the 0.188 in. hole and that the
needle is approximately centered in the hole.
r If the needle is not centered in the hole, slightly adjust sensor U3, and repeat steps 7
through 14.
r If the needle is centered in the hole, go to step 16.
16. Retract the needle from the carousel.
17. Move the oscilloscope channel 1 to pin 9 (oversize tube) on U35 of the Sample
Handler card.
4.31-2 PN 4235961E
CODE WHEEL ALIGNMENT 4
18. Verify that the oversize tube holder (identified with a white insert) is in home position,
approximately centered with the slot in the instrument door.
19. Push the needle carefully through the 0.076-in. alignment hole, located 180 degrees from
the oversize tube slot.
20. Observe the oscilloscope signal.
r If the signal is high, loosen and adjust the oversize sensor until the signal goes low,
then tighten the sensor hardware.
r If the signal is low, go to step 21.
21. Retract the needle from the alignment hole.
22. Rotate the carousel clockwise until the signal goes high. Continue rotation until the
signal goes low. This is the oversize tube pierce position.
23. Extend the needle through the 0.188-in. tube pierce hole in the carousel bracket and
carrier.
24. Ensure that the needle does not rub against the edge of the 0.188-in. hole and that the
needle is approximately centered in the hole.
r If the needle is not centered in 0.188-in. hole, repeat from step 17.
r If the needle is centered, go to step 25.
25. Retract the needle.
26. Reinstall the left-side panel.
27. Turn the compressor ON (Special Functions tt Diagnostics tt Operator Diagnostics tt Fluidics
Tests tt Compressor On/Off) and verify the instrument is working correctly. Go to
Verification.
Verification
1. From the Main menu, select Sample Analysis tt Run Samples and select a Primary-mode
cycle.
2. Cycle the instrument with normal and oversize tube options to verify operation and
adjustment of code wheel and sensors.
CHECKS.
e. Run controls.
PN 4235961E 4.31-3
CODE WHEEL ALIGNMENT
4.31-4 PN 4235961E
NEEDLE REPLACEMENT 4
4.32 NEEDLE REPLACEMENT
Needle Replacement - MAXM Analyzer with Autoloader Module
Purpose
Use this procedure to replace a needle cartridge on a MAXM analyzer with Autoloader
module.
B Needle cartridge (safety clip included)
B Distilled water
B Cotton swabs
B High-quality, fragrance-free bleach (5% sodium hypochlorite)
B Clean specimen tube with a pierceable stopper
Procedure
WARNING Biohazardous material could be contained in components in this area and could cause
contamination unless handled with care. Wear protective gear and dispose of components in accordance
with your local regulations and laboratory mandated safety procedures.
Figure 4.32-1 Cleaning Solution 1. Is the needle you are replacing bent so that it
cannot pierce a specimen tube cap?
r If yes, skip to step 3.
r If no, clean the needle. Go to step 2.
ONE PART
BLEACH 2. Clean the needle:
ONE PART
DISTILLED a. Fill the clean specimen tube
WATER
(Figure 4.32-1) with a fresh solution of -
r One-part, high-quality,
fragrance-free bleach (5% sodium
5934111C hypochlorite)
r One-part, distilled water.
b. Cap the tube with a pierceable stopper.
Figure 4.32-2 Cassette into Loading Bay c. Insert the tube into a cassette and place
the cassette in the loading bay. See
Figure 4.32-2.
d. Select Special Functions tt Diagnostics tt
Operator Options tt Fluidic Tests tt Clean
Needle.
e. Follow the on-screen instructions.
f. Wait until SELECT FUNCTION appears
on the screen.
5934181F
PN 4235961E 4.32-1
NEEDLE REPLACEMENT
Figure 4.32-3 Safety Clip Installation 3. Bleed residual pressure from the system.
Select Special Functions tt Diagnostics tt
Operator Options tt Drain and Vent..
4. Power down the instrument as directed in
Power Down/Power Up the System under
Heading 4.1, GUIDELINES FOR SERVICING
THE MAXM ANALYZER.
5. Open the lower front doors.
WARNING The needle is very sharp and can inflict severe

injury. The used one could contain biohazardous material.
Handle the needle cartridge with extreme care. Always
use the safety clip to remove and install the needle
5934190G cartridge. It protects you from possible needle puncture.
B
A 6. Attach the safety clip to the front support of
the needle cartridge. See Figure 4.32-3.
a. Without squeezing the safety clip, fit the
right edge of the safety clip into the
groove on the right side of the front
support of the needle cartridge.
b. Slide the safety clip to the left until its
left edge snaps into the groove on the left
side of the front support of the needle
cartridge with a click.
c. Check that the safety clip is securely
attached to the needle assembly.
Figure 4.32-4 Needle Cartridge Removal 7. Pull out the needle cartridge. See
Figure 4.32-4.
6324001A
4.32-2 PN 4235961E
Figure 4.32-5 Needle Assembly Location 8. Use a cotton swab and distilled water to clean
Grooves the upper and lower grooves where the
needle assembly fits. See Figure 4.32-5.
5934-116
Figure 4.32-6 Needle Assembly Tubing

WARNING During this procedure, always direct the
needle cartridge opening away from you to prevent
accidental injury. A safety clip comes attached to the new
needle cartridge. If the safety clip is missing, attach one
to the new needle cartridge before proceeding.
9. Connect the aspiration line (Figure 4.32-6):

LINE 1 a. Remove the aspiration line (tube not
numbered) from the old needle
ASPIRATION assembly, using pliers to push the line up
LINE and off of its fitting. Be careful not to cut
LINE 3
the tubing.
5934192G
(FATTEST TUBING) b. Transfer the aspiration line to the new
needle assembly.
ATTENTION: The needle assembly is available both
with and without needle lines 1 and 3.
10. If the new needle assembly does not include
lines 1 and 3 -
a. Carefully remove needle lines 1 and 3
from the old needle cartridge
(Figure 4.32-6), using pliers to push
them up and off the fittings.
b. Transfer needle lines 1 and 3 to the
fittings on the new needle cartridge.
PN 4235961E 4.32-3
NEEDLE REPLACEMENT
Figure 4.32-7 Needle Tubing Quick 11. If the new needle assembly includes lines 1
Disconnects and 3 -
a. Disconnect the old needle assembly’s
lines 1 and 3 at the quick disconnects
located on the left side of the Pump
module. See Figure 4.32-7.
Note: Do not remove these lines from
the fittings at the back of the needle
cartridge.
5934164C b. Connect the new needle assembly’s lines
1 and 3 to their corresponding quick
disconnect fittings.
12. Dispose of the used needle assembly
according to the customer’s laboratory
protocol.
Figure 4.32-8 Needle Assembly Installation 13. Slide in the new needle cartridge until it
clicks into place (Figure 4.32-8).
Note: The lock spring at the bottom of the
needle assembly is slightly raised when the
needle assembly is properly inserted.
6324002A
Figure 4.32-9 Safety Clip Removal 14. Remove the safety clip (Figure 4.32-9):
a. Slightly squeeze the safety clip to move it
a little to the right to separate the left
side of the clip from the needle assembly.
A b. Continue to move the clip to the right to
free it from the needle assembly.
15. Close the lower front door and power up the
instrument.
16. Verify the instrument is working correctly. Go
to Verification at the end of this section.
B
5934194G
4.32-4 PN 4235961E
Needle Replacement - MAXM Analyzer with Rotary Cap-Pierce Module
Purpose
Use this procedure to replace a needle on a MAXM analyzer with a Rotary Cap-Pierce module.
B Needle assembly
B Distilled water
B High-quality, fragrance-free bleach (5% sodium hypochlorite)
B Clean specimen tube with a pierceable stopper
Procedure
WARNING Biohazardous material could be contained in components in this area and could cause
contamination unless handled with care. Wear protective gear and dispose of components in accordance
with your local regulations and laboratory mandated safety procedures.
Figure 4.32-10 Cleaning Solution 1. Is the needle you are replacing bent so that it
cannot pierce a specimen tube cap?
r If yes, skip to step 3.
r If no, clean the needle. Go to step 2.
ONE PART
BLEACH
2. Clean the needle:
ONE PART
DISTILLED
a. Fill the clean specimen tube
WATER (Figure 4.32-10) with a fresh
solution of -
r One-part, high-quality,
5934111C
fragrance-free bleach (5% sodium
hypochlorite)
r One-part, distilled water.
b. Cap the tube with a pierceable stopper.
c. Select Special Functions tt Diagnostics tt
Operator Options tt Fluidic Tests tt Clean
Needle.
d. Follow the on-screen instructions.
e. Wait until SELECT FUNCTION appears
on the screen.
PN 4235961E 4.32-5
NEEDLE REPLACEMENT
Figure 4.32-11 Exit Tray Removal 3. Bleed residual pressure from the system.
Select Special Functions tt Diagnostics tt
Operator Options tt Drain and Vent.
4. Power down the instrument as directed in
Power Down/Power Up the System under
Heading 4.1, GUIDELINES FOR SERVICING
THE MAXM ANALYZER.
5. Open the lower front door.
6. Remove the exit tray. See Figure 4.32-11.
a. Squeeze the clip under the exit tray.
5934119C
b. Slide the exit tray toward you and off the
bracket.
Figure 4.32-12 Needle Tubing Quick 7. Twist and disconnect the white fittings of
Disconnects
needle tubings 1 and 3 at the quick
disconnect panel. See Figure 4.32-12.
5934164C
Figure 4.32-13 LOCK Lever 8. Pull the LOCK lever toward you and down.
See Figure 4.32-13.
9. Push the flat steel spring clip down until it
stops.
595934166C
4.32-6 PN 4235961E
Figure 4.32-14 Needle Assembly Removal
WARNING The needle is very sharp and can inflict severe
injury. The used one could contain biohazardous material.
Handle the needle cartridge with extreme care. Always
use the safety clip to remove and install the needle
SPRING cartridge. It protects you from possible needle puncture.
CLIP
10. Slide the needle assembly out:

a. Grasp the bellows collar with the thumb
and forefinger of your right hand.
BELLOWS b. Grasp the white handle at the other end
COLLAR
of the bellows with the thumb and
forefinger of your left hand.
c. Pull the collar and white handle down
simultaneously. See Figure 4.32-14.
5934122G
Figure 4.32-15 Needle Assembly 11. Disconnect the aspiration line from the old
Orientation
needle assembly and attach the line to the
new needle assembly.
12. Dispose of the used needle assembly
according to the customer’s laboratory
protocol.
13. Orient the needle assembly correctly. Hold it
with the largest tubing on the bottom and the
white handle pointing down as shown in
5934124E
Figure 4.32-15.
Figure 4.32-16 Needle Assembly Alignment 14. Align the needle assembly with the
instrument slots:
a. Put the three tubing fittings in the large
center slot of the black base bracket.
b. Hold the white handle in your left hand.
Stretch the bellows until you are able to
align the white slide clip with its guide
slot. See Figure 4.32-16.
5934-125
PN 4235961E 4.32-7
NEEDLE REPLACEMENT
Figure 4.32-17 Slots 15. Slide both top and bottom ends of the bellows
up into their slots simultaneously. See
Figure 4.32-17.
r The white clip slides home and feels
firm. Make sure that both front and back
sides of clip are in their tracks.
r The bottom end of the needle assembly
slides in its slot. The needle is parallel
with the edge of the black bracket
mounting.
5934126E
Figure 4.32-18 Needle Assembly 16. Fix the needle assembly in place
Installation
(Figure 4.32-18):
a. Push up a little on the bottom part of the
steel spring clip to relieve its tension.
Make sure that two round braces are
positioned through the keyholes in the
bracket.
b. Slide the spring clip upward until it can
go no further.
c. Push the LOCK lever up and into place.
5934129E
Figure 4.32-19 Needle Assembly Quick 17. Connect the new needle tubing. Connect the
Disconnects fittings of needle tubings 1 and 3 to the quick
disconnect. See Figure 4.32-19.
5934164C
4.32-8 PN 4235961E
Figure 4.32-20 Exit Tray Reinstallation 18. Replace the tube exit tray. Squeeze open the
tray clip and slide onto the bracket until it
can go no further. See Figure 4.32-20.
19. Close the lower front door and power up the
instrument.
20. Verify the instrument is working correctly. Go
to Verification.
5934136C
Verification
1. Select Diluter Functions tt Prime Reagents tt Diluent.
2. When priming is finished, open the lower front door and check for possible tubing leaks.
CHECKS.
e. Run controls.
PN 4235961E 4.32-9
NEEDLE REPLACEMENT
4.32-10 PN 4235961E
RINSE BLOCK ADJUSTMENT 4
4.33 RINSE BLOCK ADJUSTMENT
B Service Disk
Procedure
3. If Select Function does not appear on the DMS, press Ñ to stop the active function.
5. Energize LV20, LV40 and LV45:
a. Use æç to select LV20 (Probe Clean), and press Û.
b. Use æç to select LV40 (Probe Vacuum), and press Û.
c. Use æç to select LV45 (Diff Waste), and press Û.
d. Press Ê to energize the solenoids. The solenoids remain energized for 10 minutes.
CAUTION Screwing in the Allen screw (Figure 4.33-1 or Figure 4.33-2) while the rinse block is pulled down
can groove the plastic stop that determines the final rinse block position, causing erratic movement of the
rinse block. DO NOT screw the Allen screw IN (clockwise) while the rinse block is pulled down.
Figure 4.33-1 Rinse Block Adjusting Screw, Old BSV Configuration
Allen
screw
A A
MF 2 20 21 22
Rinse
block
5961047D
PN 4235961E 4.33-1
RINSE BLOCK ADJUSTMENT
Figure 4.33-2 Rinse Block Adjusting Screw, New BSV Configuration
Allen
screw
Rinse
block
5961235D
6. Adjust the Allen screw in the front panel (Figure 4.33-1 or Figure 4.33-2) to align the
center tubing on the rinse block with the hole in the back side of the aspirator tip. Make
slight adjustments and check the position until the position is correct.
r Clockwise (IN) raises the position of the rinse block.
r Counterclockwise (OUT) lowers the position of the rinse block.
Verification
2. On instruments with an old BSV configuration, do the BSV and BSV Housing Verification
procedure as directed under Heading 4.29, BSV AND BSV HOUSING/ACTUATOR
DISASSEMBLY/REPLACEMENT.
5. Run controls.
4.33-2 PN 4235961E
AUTOLOADER MODULE REMOVAL 4
4.34 AUTOLOADER MODULE REMOVAL
Purpose
Use this procedure to remove and install an Autoloader module.
B Phillips-head screwdriver
B 5/16-in. hex-nut driver
Removal
2. Open the upper and lower front doors of the Main Unit, and remove the front decorative
snap-on panels from the Autoloader module.
3. Using the safety clip, remove the piercing-needle cartridge and rest it on the Pump
module.
4. Disconnect the green stripe tubing at the #2 Luer lock from the front quick disconnect,
QD14.
5. Loosen the two Phillips-head screws mounted along the front base of the Autoloader
module to release the retaining brackets that secure the module into the chassis.
6. Pull the Autoloader module forward and slide it out about half-way.
7. Loosen the captive screw located on the top rear of the bar-code scanner cover.
8. Remove the bar-code scanner cover and rest it to the left of the Main Unit.
9. Unplug the gray bar-code scanner cable at the top of the scanner mechanism and rest it
across the BSV module.
10. Remove the 5/16-in. hex nut securing a ground wire to E50 on the left side of the
Autoloader module, then disconnect the ground wire.
11. Gently pull the Autoloader module forward until it stops.
12. Locate the two guide rails underneath the Autoloader module, then press the spring clips
in (up) while continuing to pull the module forward. See Figures 4.34-1 and 4.34-2.
13. Carefully remove the Autoloader module from the chassis.
Note: You may have to disconnect the aspirate tubing from the piercing needle to allow
the module to come out all the way.
14. Disconnect the cables connected to the Autoloader Interface card at the rear of the
Autoloader Module:
r Unplug the 50-pin ribbon cable from J9 on the Autoloader Interface card linking the
Autoloader module to the Sample Handler card.
r Unplug the 2-pin cable at J17 on the Autoloader Interface card linking the STOP
switch to the Autoloader Interface card.
Installation
1. To reinstall the Autoloader module, follow steps 1 through 14 above, in reverse order.
PN 4235961E 4.34-1
AUTOLOADER MODULE REMOVAL
Figure 4.34-1 Autoloader Module Guide Rails
Guide
rails
Spring
clips
5961228D
Figure 4.34-2 Autoloader Module Removal
5961068E
Verification
4.34-2 PN 4235961E
AUTOLOADER MODULE REMOVAL 4
2. On instruments with a BSV with air cylinders, do the BSV and BSV Housing Verification
procedure as directed under Heading 4.29, BSV AND BSV HOUSING/ACTUATOR
DISASSEMBLY/REPLACEMENT.
5. Run controls.
PN 4235961E 4.34-3
AUTOLOADER MODULE REMOVAL
4.34-4 PN 4235961E
ROCKER BED ASSEMBLY REMOVAL 4
4.35 ROCKER BED ASSEMBLY REMOVAL
Purpose
Use this procedure to remove and install a rocker bed assembly.
B 1/4-in. hex-nut driver
B Fine needle-nose pliers or E-clip spreaders
Removal
1. Power down the instrument and remove the Autoloader module as instructed under
Heading 4.34, AUTOLOADER MODULE REMOVAL.
2. Push the right elevator up slightly and remove the small E-clip (Figure 4.35-1) that
holds the rocker bed linkage to the rocker motor shaft located under the rocker bed near
the base of the Autoloader housing.
3. Remove the rocker bed linkage (Figure 4.35-1) from the rocker motor shaft.
Figure 4.35-1 Rocker Bed Removal
ROCKER BED
LINKAGE
ROCKER
MOTOR
E-CLIP
PIVOT
HUB 5961071E
PN 4235961E 4.35-1
ROCKER BED ASSEMBLY REMOVAL
4. Locate and remove the three 1/4-in. hex nuts on the outer left side of the Autoloader
module, then pull out the silver rocker bed pivot pin. See Figure 4.35-2.
Note: The left end of the rocker bed contains a spring washer on the rocker bed pivot
pin. When removing this washer, pay close attention to its location.
Figure 4.35-2 Rocker Bed Hardware
SPRING
WASHER
PIVOT
PIN
PIVOT
PIN
5961201C
5. Locate and remove the three 1/4-in. hexnuts on the outer right side of the Autoloader
module, then pull out the silver rocker bed pivot pin. See Figure 4.35-2.
Note: The right end of the rocker bed does not contain a spring washer.
6. Unplug the black Hi-flex ribbon cable from P20 on the Autoloader Interface card.
7. Carefully slide the Autoloader Interface card out from underneath the left side of the
rocker motor assembly. Be careful not to damage the red and black wires connected to
the rocker motor.
8. Lift the rocker bed assembly forward and out of the Autoloader module.
4.35-2 PN 4235961E
ROCKER BED ASSEMBLY REMOVAL 4
Installation
1. To reinstall the rocker bed assembly, follow step 1 through 8 above, in reverse order.
Ensure that the curved (convex) side of the spring washer is closest to the rocker bed.
2. Reinstall the Autoloader module. See Installation under Heading 4.34, AUTOLOADER
MODULE REMOVAL.
3. Verify the instrument is working correctly. Go to Verification below.
Verification
5. Run controls.
PN 4235961E 4.35-3
ROCKER BED ASSEMBLY REMOVAL
4.35-4 PN 4235961E
ELEVATOR PLATFORM STEPPER MOTOR REPLACEMENT 4
4.36 ELEVATOR PLATFORM STEPPER MOTOR REPLACEMENT
Purpose
Use this procedure to remove and replace either the load or unload elevator platform stepper
motors.
B 5/16 in. hex-nut driver
Removal
2. Remove the rocker bed assembly as instructed under Heading 4.35, ROCKER BED
ASSEMBLY REMOVAL, beginning at step 2.
3. On the under side of the rocker bed, locate and remove the stepper motor from its
mounting bracket:
a. Unscrew and remove the two diagonally placed 5/16-in. hex nuts surrounding the
motor.
b. Unscrew and remove the corresponding Phillips-head screws. These screws are
accessed through two holes on the top of the respective elevator platform at the top
side of the rocker bed.
Note: It may be necessary to manually lift the elevator to view the screw heads.
c. Gently pull the motor down and off the lead-screw (threaded shaft) to remove
the motor.
Installation
1. Install the stepper motor in the rocker bed assembly following step 3 a through 3 c above
in reverse order.
2. Reinstall the rocker bed assembly. See Installation under Heading 4.35, ROCKER BED
ASSEMBLY REMOVAL
MODULE REMOVAL.
Verification
PN 4235961E 4.36-1
ELEVATOR PLATFORM STEPPER MOTOR REPLACEMENT

5. Run controls.
4.36-2 PN 4235961E
TUBE AVAILABLE SENSOR ASSEMBLY REPLACEMENT 4
4.37 TUBE AVAILABLE SENSOR ASSEMBLY REPLACEMENT
Purpose
Use this procedure to remove and replace the tube available sensor assembly.
B Small flathead screwdriver
Removal
2. Remove the rocker bed assembly as instructed under Heading 4.35, ROCKER BED
ASSEMBLY REMOVAL, beginning at step 2.
3. Remove the Rocker Bed Interface card to access the tube available sensor assembly:
a. Turn the rocker bed over to expose the under side.
b. Remove the two screws securing the Rocker Bed Interface card to the rocker bed
assembly.
c. Disconnect the Rocker Bed Interface card and remove it.
4. Locate the clip-in tube available sensor assembly and remove the assembly by either of
the following methods:
r Push the sensor from the top side of the rocker bed.
r Pry the sensor with a small flathead screwdriver from the under side of the
rocker bed.
Installation
1. On the under side of the rocker bed, press the tube available sensor assembly (with a
new sensor if needed) back into its keyed sensor hole.
2. Ensure that the sensor "springs" in and out smoothly.
3. Reconnect and reinstall the Rocker Bed Interface card.
4. Reinstall the rocker bed assembly. See Installation under Heading 4.35, ROCKER BED
ASSEMBLY REMOVAL.
MODULE REMOVAL.
Verification
PN 4235961E 4.37-1
TUBE AVAILABLE SENSOR ASSEMBLY REPLACEMENT
5. Run controls.
4.37-2 PN 4235961E
BAR-CODE READER DECODER CARD CONFIGURATION 4
4.38 BAR-CODE READER DECODER CARD CONFIGURATION
Purpose
Use the following procedure to reconfigure the Microscan™ Bar-Code Reader Decoder card
for MAXM analyzer customers under the following conditions.
r The customer has bar-code specifications that deviate from the current default settings
for code types. If that is the case, you must reconfigure the settings on the Bar-Code
Reader Decoder card according to the customer’s requirements.
r The customer has a COULTER STKS analyzer in the same institution and uses the
"COULTER CODABAR" Standard. If that is the case, you must reconfigure the Bar-Code
Reader Decoder card so that C/D Aim = DISABLED or C/D Type = MOD-16.
Note: Configuration changes apply only to Code Types. See Table A.4-1, Bar-Code Reader
Decoder Card Code Types Default Settings.
ATTENTION: The Communications, Operations, and User Outputs settings for the Bar-code
Decoder card are set at the factory and are NOT to be changed from the default settings.
These default settings are listed in the following tables for your reference:
r Table A.4-2, Bar-Code Reader Decoder Card Communications Default Settings
r Table A.4-3, Bar-Code Reader Decoder Card Operations Default Settings
r Table A.4-4, Bar-Code Reader Decoder Card User Outputs Default Settings
B Service Disk
B Bar-Code Reader Communications cable, PN 6028275
Procedure
Connecting the Bar-Code Communications Cable

2. Remove the left-side panel and, if the Sample Handler card is covered, remove the cover
to access the Sample Handler card area.
3. Check the area next to the blood/bubble detector connectors on the Sample Handler card
for a 9-pin serial connector.
r If a 9-pin serial connector is present, go to step 4.
r If a 9-pin serial connector is not present:
t If the instrument has a Rotary Cap-Pierce module, go to step 5.
t If the instrument has an Auotloader module, go to step 6.
PN 4235961E 4.38-1
BAR-CODE READER DECODER CARD CONFIGURATION
4. Install the Bar-Code Reader Communications cable:

a. Connect one end of the Bar-Code Reader Communications cable to the 9-pin serial
connector J8 next to the Sample Handler card.
b. Connect the other end of the Communications cable to the 9-pin serial connector
(COM1) on the rear of the DMS.
c. Go to step 9.
5. If the instrument has a Rotary Cap-Pierce module:
a. Open the lower front door to access the Bar-Code Reader Decoder card located
behind the panel of the Rotary Cap-Pierce module.
b. Go to step 7.
6. If the instrument has an Autoloader module
a. Partially remove the Autoloader module as instructed under Heading 4.34,
AUTOLOADER MODULE REMOVAL to access the Bar-Code Reader Decoder card.
Note: It is not necessary to completely remove the Autoloader module or to
disconnect the rear cables of the module.
b. Go to step 7.
7. Install the Bar-Code Reader Communications cable:
a. Connect one end of the Bar-Code Reader Communications cable to the 9-pin serial
port, J8, on the top, left side of the Bar-Code Reader Decoder card.
b. Carefully route the Communications cable out the left side (near the Sample
Handler card) of the instrument.
c. Connect the other end of the Bar-Code Reader Communications cable to the 9-pin
serial connector (COM 1) on the rear of the DMS.
8. On an instrument with an Autoloader module, slide the Autoloader module back into
the instrument, but do not secure it.
9. Close the lower front door if it is open, and power up the instrument.
Selecting the Settings for the Microscan™ Bar-Code Reader Decoder Card
1. Access the Service Disk’s software options as directed in Using the Service Disk Software
on a DMS under Heading 4.2, USING THE SERVICE DISK.
2. Press È to access the Main menu.
4.38-2 PN 4235961E
BAR-CODE READER DECODER CARD CONFIGURATION 4
3. Select Utilities tt Communications to start the SIMPCOM communications program.
a. When the program first loads, ensure the communications settings are set to these
default settings:
r COM 1
r BAUD RATE =1200
r PARITY = ODD
r DATA BITS = EIGHT
r STOP BITS = ONE
Note: These default communications settings are required for communication to the
Bar-Code Reader Decoder card.
b. If the settings are not correct, press Ê Params and select the appropriate settings.
4. Press Í Options, enable O-Show Outgoing Chars, and then press È.
ATTENTION: The Configuration Program is case sensitive when dealing with the
screen-movement keys. All the keys you press to move about the screen must be in upper
case. If you press a screen-movement key in lower case, the command is not processed.
5. Type <D> (the less than symbol, upper-case D, the greater than symbol) to bring up the
Microscan Systems, Inc. Configuration Program Main Menu. Table 4.38-1 shows you
what keys to use to move through the menus and make selections in the
Configuration Program.
Table 4.38-1 Moving Around the Microscan Screens
Entry Function
<D> Brings up Microscan Main Menu.
È The screen displays Exit or Main Menu (E, M).
Pressing E exits you from program. The prompt, Do you want to save
changes for power on? appears. Press Y. Y = Yes, N = No.
M Returns you to Microscan Main Menu.
Û (carriage return) Selects item indicated by cursor. This acts in a toggle fashion.
B Returns you to previous menu item.
ß or N Moves cursor to next menu item.
ATTENTION: With the Microscan program, any character or keyboard command that is typed is
processed. Consequently, if the wrong characters are entered, deleting/backspacing will not
initiate the program. If you make a keying error, you may need to reboot the DMS and
begin again.
6. Select CODE TYPES from the Microscan Main Menu (press ß until CODE TYPES
appears next to the cursor, then press Û)
The screen displays the Current Settings for Code Types menu. The default settings are
listed in Table A.4-1, Bar-Code Reader Decoder Card Code Types Default Settings.
7. Change the code-type settings as required.
PN 4235961E 4.38-3
BAR-CODE READER DECODER CARD CONFIGURATION
8. When you have changed all the necessary code-type settings, press È. The screen
displays Exit or Main Menu (E, M).
9. Select the appropriate choice from the two choices displayed.
ATTENTION: You do not receive a visual confirmation letting you know that your changes
were saved. The Bar-Code Reader Decoder card "beeps" when you use the save command
correctly and your changes are saved. If you do not hear the "beep," you did not do the
command correctly and you must start the program over again.
r Press E to exit the program and save your changes.
The screen displays Do you want to save changes for power on? Press Y. Make sure
you press the upper-case Y.
r Press M to return to the Main Menu without saving your changes.
10. When finished, remove the Service Disk from the drive.
11. To verify that the new settings were saved as the default settings for power up, turn the
Standby/Reset switch to Standby (0) for 15 seconds, then back to Ready (I).
12. To verify that the bar-code reader is operating correctly, cycle several bar-code labeled
specimen tubes and ensure the labels are read correctly.
13. If the bar-code reader is not reading the labels correctly, the new settings were not saved.
Repeat this procedure.
Reassembling the Instrument for Operation

2. Disconnect and remove the Bar-code Reader Communication cable from the Main Unit
and the DMS.
3. On an instrument with an Autoloader module and without the 9-pin serial connector J8
next to the Sample Handler card, install and secure the Autoloader module using steps 2
through 9 of the Removal procedure under Heading 4.34, AUTOLOADER MODULE
REMOVAL, in reverse order.
4. Reinstall the Sample Handler card cover and the left-side panel, and close the doors.
5. Power up the instrument and verify it is working correctly. Go to Verification.
Verification
5. Run controls.
4.38-4 PN 4235961E
BAR-CODE SCANNER ALIGNMENT (FOR AUTOLOADER MODULE ONLY) 4
4.39 BAR-CODE SCANNER ALIGNMENT (FOR AUTOLOADER MODULE ONLY)
Purpose
Use the following procedure to adjust the position of the bar-code scanner to provide proper
positioning of the laser beam across the sample tube and cassette position bar-code labels.
The bar-code labels are scanned as the rocker bed rocks to mix the sample at the pierce
position, thus the label is read on-the-fly.
This procedure should only be necessary if the scanner has been replaced or completely
removed then reinstalled. It should be verified if the unit is experiencing frequent No Read
errors.
B Service Disk
B Bar-Code Reader Communications cable, PN 6028275
B Cassette loaded with bar-code labeled tubes
Procedure
Connecting the Bar-Code Communications Cable

1. Power down the instrument and partially remove the Autoloader module to gain access
to the Bar-Code Reader Decoder card mounted on the rear wall of the autosampler cavity.
Follow steps 1 through 9 under Heading 4.34, AUTOLOADER MODULE REMOVAL.
Note: It is not necessary to completely remove the Autoloader module or to disconnect
the rear cables of the module.
2. Connect the Bar-Code Reader Communications cable:
a. Connect one end of the Communications cable to the 9-pin serial port, J8, on the
left side of the Bar-Code Reader Decoder card.
b. Remove the left-side panel of the instrument, and carefully route the
Communications cable out the left side (near the Sample Handler card) of the
instrument.
c. Connect the other end of the Communications cable to the top 9-pin serial
connector on the rear of the DMS or an equivalent laptop computer.
3. Reconnect the gray bar-code scanner cable to the top of the scanner mechanism.
4. Leave the Autoloader module pulled out far enough to access the three Phillips-head
adjustment screws that secure the bar-code scanner assembly to its support bracket on
the Autoloader housing. See Figure 4.39-1.
5. Bypass the lower front door switch with a door magnet (or the system cannot boot) and
power up the instrument.
PN 4235961E 4.39-1
BAR-CODE SCANNER ALIGNMENT (FOR AUTOLOADER MODULE ONLY)
Aligning the Bar-Code Scanner

1. After the DMS displays the MAXM analyzer picture, initiate the bar-code rate scan test:
a. Insert the current Service Disk into drive A, and press the RESET switch on the DMS,
or equivalent, to boot the current Service Disk.
b. At the A:\> prompt, the following appears:
Com = 1, baud = 1200, data = 8, parity = o, stop = 1
Type <Ctrl> Z to abort!
c. Press È. A small arrow (→) and the word Password appears.
d. Using all capital letters, type HADES to gain access to the Cap-Piercer Test menu.
e. At the Cap Piercer Test menu, press M to download the Sample Handler
Monitor c196 menu to the DMS.
f. Press 6 to download the Diagnostics menu.
g. Press 8 to enter the Test Barcode Reader option. The bar-code scanner turns ON.
Figure 4.39-1 Bar-Code Scanner Adjustment Screws
BAR-CODE
SCANNER
ADJUSTMENT
SCREWS
5961070C
2. Insert five bar-code labelled specimen tubes into a cassette and place the cassette onto
the rocker bed on top of the load elevator (elevator in the down position).
3. Using the cassette index mechanism, manually advance the cassette until the specimen
tube in position one is at the pierce position, relative to the end of the needle bellows and
dead plate on the piercing station.
4. Manually position the rocker bed 22.5 degrees forward, relative to the level and pierce
positions. This position is halfway between the horizontal and pierce positions of the
rocker bed. (The pierce position is 45 degrees from the horizontal position).
5. Slightly loosen the three Phillips-head adjustment screws that secure the bar-code
scanner assembly to its support bracket on the Autoloader housing (shown in
Figure 4.39-1), and loosen the small black Phillips-head screw (not shown) that secures
the scanner to its left-hand base support bracket.
4.39-2 PN 4235961E
BAR-CODE SCANNER ALIGNMENT (FOR AUTOLOADER MODULE ONLY) 4
6. Carefully shift the bar-code scanner assembly in its bracket until the red scanner beam
(scan line) is positioned:
r About in the middle of the gap on the top of the cassette that exposes the bar-code
label on the specimen tube.
r Through the center of the cassette label.
7. View the DMS screen, or equivalent, as the bar-code rate scan test is scrolling.
r The left- and right-hand percentage (%) columns indicate the capability of the
scanner to read both the specimen tube and cassette labels, respectively, at a given
position.
r A 100% indication on the DMS, or equivalent, is perfect and can easily be achieved
with the Interleave 2 of 5 specimen tube bar-code labels and Code 128 cassette
labels provided by Coulter Corporation.
Note: Remember that throughout this procedure the rocker bed should be maintained at
the 22.5 degree angle as described in step 4 above.
8. When the scan line is configured as described in steps 6 and 7, carefully tighten the three
screws loosened in step 5. Ensure that the scan line does not drift away from the middle
of the label gap on the cassette.
9. Ensuring the rocker bed remains positioned at 22.5 degrees forward, manually index the
cassette to the unload elevator.
a. Verify that as each tube progresses to the pierce position the scan line is positioned
as described in step 6.
b. Verify that the bar-code read rate displayed on the DMS, or equivalent, is consistent
from tube to tube ±10%.
c. If not, repeat from step 2 until the positioning of the bar-code scanner is acceptable.
Otherwise, continue to step 10.
10. At the DMS, or equivalent, press È as often as needed to exit the bar-code rate scan test
and the HADES or Cap-Piercer Test menus. The messages backdoor closed and/or G’day
Mate appear when the program is successfully exited.
11. If you are using a DMS (not a laptop):
a. Remove the current Service Disk from drive A.
b. Using the standby/reset switch, turn the instrument OFF and then back ON.
c. Allow the instrument to boot the MAXM analyzer software.
12. Perform a Start Up as needed by the instrument.
13. Using the Run Samples option of the DMS, cycle a minimum of three cassettes
containing bar-code labelled specimen tubes and verify that all bar-code labels are read
correctly. If not, repeat from step 2 until the labels are correctly read.
Reassembling the Instrument for Operation

2. Access and disconnect the Bar-Code Reader Communication cable from the Bar-Code
Reader Decoder card and the rear of the DMS, or equivalent.
PN 4235961E 4.39-3
BAR-CODE SCANNER ALIGNMENT (FOR AUTOLOADER MODULE ONLY)
3. Install and secure the Autoloader module using steps 2 through 9 of the Removal
procedure under Heading 4.34, AUTOLOADER MODULE REMOVAL, in reverse order.
4. Remove the bypass magnet and close the doors.
5. Power up the instrument and verify the instrument is working correctly. Go to
Verification.
Verification
5. Run controls.
4.39-4 PN 4235961E
ROCKER BED LINKAGE ADJUSTMENT 4
4.40 ROCKER BED LINKAGE ADJUSTMENT
General
The adjustable rocker bed linkage converts the rotational motion of the dc rocker motor into
the rocking motion of the bed (Figures 4.40-1 and 4.40-2).
Figure 4.40-1 Rocker Bed Linkage
ROCKER BED
LINKAGE
ROCKER
MOTOR
E-CLIP
PIVOT
HUB 5961071E
If the link is too long, the bed travels beyond the pierce position (greater than 45 degrees).
Under this condition, a tube extending beyond the cassette envelope interferes with the
operation of the dead plate and can cause the cassette to lift or walk. In addition, the piercing
needle may enter the stopper above its center because the specimen tube is positioned
improperly in front of the needle.
If the link is too short, the bed does not rock far enough to the forward position (45 degrees)
and may prevent the bed from locking into position. Occasionally, a shorter link allows the
bed to jam in the locked position, rendering it difficult to unlock the bed. Also, the piercing
needle may enter the stopper below its center because the sample tube is positioned
improperly in front of the needle.
An adequately adjusted link must be close to top-dead-center (Figure 4.40-2) when the bed is
locked. The allowable bed over-travel is one degree maximum and the under-travel is half a
degree maximum.
PN 4235961E 4.40-1
ROCKER BED LINKAGE ADJUSTMENT
Figure 4.40-2 Rocker Bed Linkage Adjustment

ROCKER BED
LINKAGE
CONNECTS TO
UNDERSIDE OF
ROCKER BED
1
PIVOT HUB
CONNECTS TO ADJUSTABLE
ROCKER MOTOR ROCKER BED
SHAFT AT CENTER LINK ("X")
OF HUB
E-CLIP SECURES
ROCKER BED LINK
TO PIVOT HUB
PIVOT HUB
3
2
A. IF "X" IS TOO LONG, BED TRAVELS BEYOND THE PIERCE POSITION (45 DEGREES).
B. IF "X" IS TOO SHORT, BED ROCKS LESS THAN 45 DEGREES AND MAY NOT LOCK IN PLACE.
C. IF "X" IS CORRECT, BED LINKAGE IS PERFECTLY ALIGNED WITH POINTS 1, 2,
AND 3 (TOP-DEAD-CENTER). 5961072C
B Ignition pliers
B Autosensor Test card and cable
B Small hemostats
B Service Disk
Procedure
2. Remove the left-side panel from the Main Unit to access the Sample Handler card. For
location of the Sample Handler card, see Figure 2.1-5.
3. Connect the Autosensor Test card:
a. Disconnect the ribbon cable plugged into J15 on the Sample Handler card.
b. Plug the P15 connector of the removed ribbon cable (step a) into the large
connector on the Autosensor Test card.
c. Connect the Autosensor Test card accessory cable to connector J15 on the Sample
Handler II card to connect the Autosensor Test card in series with the Autoloader
Interface and Sample Handler cards.
4.40-2 PN 4235961E
ROCKER BED LINKAGE ADJUSTMENT 4
4. Partially remove the Autoloader module as instructed under Heading 4.34,
AUTOLOADER MODULE REMOVAL, to gain access to the solenoids on the left rear of
the Autoloader housing.
Note: It is not necessary to completely remove the Autoloader module or to disconnect
the rear cables of the module.
5. Bypass the lower front door switch with a magnet and power up the Main Unit. Allow
the system to boot.
6. Manually rock the rocker bed forward until the pierce position (S16) LED on the
Autosensor Test card changes state (switches to OFF). Hold the rocker bed in this
position.
7. Use the bed lock solenoid, LV84, located on the rear of the Autoloader housing (top
solenoid on the left rear).
a. Press and hold the orange button on LV84 to activate the bed lock cylinder.
b. Using the hemostats, crimp the green stripe tubing at the bottom of the bed lock
cylinder to maintain pressure to the cylinder.
c. Release the orange button on LV84. The bed should stay locked in position.
8. Examine and adjust, if necessary, the rocker bed linkage for top-dead-center alignment
with respect to positions 1, 2, and 3 detailed in Figure 4.40-2.
9. Repeat steps 6 through 8 above as needed to obtain correct adjustment of the rocker
bed linkage.
11. From the Service Options menu, select Autoloader Tests tt Autoloader Test Routine.
12. Place a loaded cassette into the loading bay to initiate the Autoloader Test Routine and
monitor the instrument:
a. Ensure that the rocking, stopping, locking, and unlocking sequences of the rocker
bed are smooth.
b. Ensure that the bed lock does not jam and that extended tubes in the cassette do not
interfere with the dead plate.
c. Ensure that the Autoloader Test Routine is completed with no error codes. If not,
troubleshoot as required to resolve the problem and repeat the test.
13. Reassemble the instrument:
a. Power down the instrument.
b. Disconnect the Autosensor Test card from the Sample Handler card.
c. Reconnect the ribbon cable connector, P15, to J15 on the Sample Handler card.
d. Reinstall and secure the Autoloader module using steps 2 through 9 of the Removal
procedure under Heading 4.34, AUTOLOADER MODULE REMOVAL, in reverse
order.
e. Remove the bypass magnet from the lower door switch and close the doors.
14. Power up the instrument and ensure it is working correctly. Go to Verification.
PN 4235961E 4.40-3
ROCKER BED LINKAGE ADJUSTMENT
Verification
5. Run controls.
4.40-4 PN 4235961E
SPECIMEN TUBE STOPPER PIERCE-PROXIMITY ADJUSTMENT 4
4.41 SPECIMEN TUBE STOPPER PIERCE-PROXIMITY ADJUSTMENT
General
The positions of the piercing needle-drive cylinder and tube-ram cylinder, with respect to the
position of the rocker bed in the forward position, are major determining factors where
specimen-tube stopper is penetrated by the piercing needle. Refer to Figure 4.41-1 detailing
the positions of these components.
Figure 4.41-1 Specimen Tube Stopper Piercing Proximity

1. The tube ram and needle-drive
cylinder rods are directly across from
TUBE each other and respectively
RAM
perpendicular to the fixed rocker bed.
2. The mounting screws for the piercing
needle-drive cylinder provide limited
adjustment of the relationship
described above.
3. Although the mounting screws for
PERPENDICULAR the tube-ram cylinder also provide
ROCKER BED ASSEMBLY some "play" in its adjustment, the
piercing needle-drive cylinder has the
greatest effect on specimen-tube
pierce proximity.
TOP TOP TOP 4. The relationship of the above
TILTED TILTED
TOO FAR TOO FAR components affects only the
LEFT NEEDLE- RIGHT
DRIVE left-to-right (horizontal)
CYLINDER specimen-tube stopper pierce
BOTTOM
proximity, whereas the forward
position of the rocker bed affects the
ADJUSTED TOO ADJUSTED TOO up/down (vertical) pierce proximity.
FAR LEFT FAR RIGHT
5. The stopper should be pierced in the
center ± 0.79 mm (1/32 in.)
maximum deviation from the center.
A X B
SAMPLE TUBE STOPPER PIERCING PROXIMITY
X = PERFECT CENTER, NEEDLE-DRIVE CYLINDER ADJUSTMENT GOOD.

A = TOO FAR LEFT, NEEDLE-DRIVE CYLINDER TILTED OR ADJUSTED
TOO FAR TO LEFT.
B = TOO FAR RIGHT, NEEDLE-DRIVE CYLINDER TILTED OR ADJUSTED
TOO FAR TO RIGHT.
5961073C
Purpose
This procedure is required if the piercing needle-drive cylinder needs to be removed and/or
replaced, or if the unit has been experiencing piercing errors.
PN 4235961E 4.41-1
SPECIMEN TUBE STOPPER PIERCE-PROXIMITY ADJUSTMENT
B Fresh, unpierced, specimen tubes
B Service Disk
Preliminary Procedure
1. If replacement of the cylinder is required, prior to removing the existing needle-drive
cylinder, mark its position with a marker. (Obtain a footprint of its location).
2. As required, install the new cylinder using the footprint as a reference for positioning.
3. Ensure that the piercing needle is not bent.
4. Proceed with the Horizontal Pierce-Proximity Adjustments and Vertical Pierce-Proximity
Adjustments.
Horizontal Pierce-Proximity Adjustments

2. From the Service Options menu, select Autoloader Tests tt Needle Position Test.
3. Load a cassette with fresh, unpierced specimen tubes and place the cassette into the
loading bay. The cassette is processed to the pierce position.
4. Press Ê Pierce and observe the piercing location. Refer to Figure 4.41-1 for an example
of the correct proximity and details concerning the adjustment decisions.
5. Press Ë Retract to retract the needle. The cassette moves to the next position.
6. Make slight adjustments to the position of the needle-drive cylinder as needed.
7. Select Needle Position Test and repeat from step 3 as often as needed to obtain the correct
horizontal-pierce proximity. The stopper should be pierced in the center of the tube
± 0.79 mm (1/32-in.) deviation on the horizontal axis.
Vertical Pierce-Proximity Adjustments

2. From the Service Options menu, select Autoloader Tests tt Needle Position Test.
3. Load a cassette with fresh, unpierced specimen tubes and place the cassette into the
loading bay. The cassette is processed to the pierce position.
4. Press Ê Pierce and observe the piercing location. Refer to Figure 4.41-1 for an example
of the correct proximity and details concerning the adjustment decisions.
5. Press Ë Retract to retract the needle. The cassette moves to the next position.
6. Repeat steps 4 and 5 for all five tubes.
7. Ensure that each tube is pierced in the center of the tube ± 0.79 mm (1/32-in.) deviation
on the vertical axis.
4.41-2 PN 4235961E
SPECIMEN TUBE STOPPER PIERCE-PROXIMITY ADJUSTMENT 4
8. If adjustment to the vertical-pierce location is needed:
a. Loosen the two outer screws that mount the bed-lock cylinder, located on the inside
right of the Autoloader housing.
r Move the bed-lock cylinder toward the back of the unit to lower the pierce-hole
location.
r Move the bed-lock cylinder toward the front of the unit to raise the pierce-hole
location.
b. Tighten the screws while holding the cylinder in position.
9. Repeat from 2 as needed to align the vertical position.
10. Ensure the instrument is working correctly. Go to Verification.
Verification
1. If you adjusted the bed-lock cylinder, check the rocker bed linkage adjustment as
directed under Heading 4.40, ROCKER BED LINKAGE ADJUSTMENT.
2. If you did not adjust the bed-lock cylinder, verify instrument performance:
CHECKS.
e. Run controls.
PN 4235961E 4.41-3
SPECIMEN TUBE STOPPER PIERCE-PROXIMITY ADJUSTMENT
4.41-4 PN 4235961E
CASSETTE INDEX MOTOR/INDEX HUB GAP ADJUSTMENT 4
4.42 CASSETTE INDEX MOTOR/INDEX HUB GAP ADJUSTMENT
Purpose
This adjustment is required if either the cassette index motor or index hub has been replaced.
B Gap Adjustment Tool, PN 1021986
B Set of Allen wrenches
Procedure
1. Loosen the two Allen screws that secure the index hub onto the motor shaft. See
Figure 4.42-1.
Figure 4.42-1 Index Motor/Index Hub Gap Adjustment
Allen
screws
Gap
Adjustment 5961074E
Tool
2. Insert the 0.318 cm (0.125 in.) Gap Adjustment Tool between the cassette index motor
and the index hub, as shown in Figure 4.42-1, to set the distance between the index
motor and index hub.
3. When the correct distance is set, tighten the Allen screws.
4. Ensure the instrument is working correctly. Go to Verification.
Verification
PN 4235961E 4.42-1
CASSETTE INDEX MOTOR/INDEX HUB GAP ADJUSTMENT

5. Run controls.
4.42-2 PN 4235961E
SWITCH/SENSOR CHECK 4
4.43 SWITCH/SENSOR CHECK
B Service Disk
Purpose
Use this procedure to verify a switch or sensor is working correctly.
Procedure
1. Activate the service options as directed under Table 4.2, USING THE SERVICE DISK.
2. From the Service Options menu, select Switch/Sensor Check.
3. Press Í Run to display the states of the switches and sensors.
4. Activate the desired switch (or sensor) as directed in Table 4.43-1, Switch/Sensor Checks
on the MAXM Analyzer with Autoloader Module, or Table 4.43-2, Switch/Sensor Checks
on the MAXM Analyzer with Rotary Cap-Pierce Module.
5. Verify the display for that switch (or sensor) changes from 0 to 1, or 1 to 0.
6. If a switch (or sensor) does not change states correctly, fix the problem and repeat this
procedure.
Table 4.43-1 Switch/Sensor Checks on the MAXM Analyzer with Autoloader Module
Switch or Sensor/ Idle

Location Reference Type Function State Action to Change State
Whole Blood, S2-whole blood 0 Press the sample bar.
Backwash Safety, S3-backwash 0 Manually move the rinse block down to
the end of travel.
Rinse Block Arm, S4 1 Manually move rinse block assembly
from the home position.
Diluent, FD6 Float or Senses when the 1 Manually move the float sensor or
See Figure A.5-7 Optical diluent container is remove diluent from the optical chamber.
empty.
Cleaner, FD7 Float or Senses when the 1 Manually move the float sensor or
See Figure A.5-7 Optical cleaning agent remove cleaning agent from the optical
container is empty. chamber.
Waste Float Senses when the waste 1
container is full. WARNING Biohazardous contamination
could occur from contact with the waste
container and its associated tubing if
not handled with care. Wear protective
gear. Avoid skin contact. Clean up spills
immediately.
Manually move the float sensor.
PN 4235961E 4.43-1
SWITCH/SENSOR CHECK
Table 4.43-1 Switch/Sensor Checks on the MAXM Analyzer with Autoloader Module (Continued)

Sheath Tank,S5-tank Float Senses when the 0 Manually drain the sheath tank, allowing
sheath tank is empty. the float sensor to drop to its lower end
of travel.
CBC Lyse, FD5 Cycle Senses when the CBC 0 If the instrument has an optical sensor,
See Figure A.5-7 counter lytic reagent container remove CBC lytic reagent from the
or Optical is empty. optical chamber.
Erythrolyse, FD3 Cycle Senses when the 0 If the instrument has an optical sensor,
See Figure A.5-7 counter Erythrolyse II (PAK remove Erythrolyse II reagent from the
or Optical LYSE) reagent optical chamber.
container is empty.
Stabilyse, FD4 Cycle Senses when the 0 If the instrument has an optical sensor,
See Figure A.5-7 counter StabiLyse (PAK remove StabiLyse reagent from the
or Optical PRESERVE) reagent optical chamber.
container is empty.
Door Interlock, S5 Magnetic Senses the front door 1 Open the front door or manually activate
See Figure 2.9-1 is opened/closed the sensor with an external magnet.
STOP Switch, S4 Switch Stops instrument after 1 Press the STOP button.
current cycle.
Load Stack Empty, S15 Optical Senses the loading bay 0 Press the sensor actuator; it is located in
See Figure 2.9-1, Figure 2.9-2 is empty. the lower rear of the loading bay.
Unload Stack Full, S11 Optical Senses the unloading 0 Press the sensor actuator; it is located in
See Figure 2.9-1, Figure 2.9-2 bay is full - five the upper right of the unloading bay.
cassettes are present.
Needle Home, S0 Hall Senses the needle is 0
See Figure 2.9-1, on CL6 (Hall effect or fully retracted to the WARNING Biohazardous contamination
effect) or Figure 2.9-2, at Optical home position. could occur from contact with the
Piercing station (optical). needle and its associated tubing if not
handled with care. Wear protective gear
and avoid skin contact.
Disconnect the tubing from CL6 and

manually move the needle from the
home position.
Needle Forward, S1 Hall Senses the needle is 1
See Figure 2.9-1, on CL6 (Hall effect or forward in the piercing WARNING Biohazardous contamination
effect) or Figure 2.9-2, at Optical position. could occur from contact with the
Piercing station (optical). needle and its associated tubing if not
handled with care. Wear protective gear
and avoid skin contact.

manually move the needle to the fully
forward position.
4.43-2 PN 4235961E

Tube Ram, S10 Hall Senses the tube ram 1 Manually move the tube ram from the
See Figure 2.9-3 effect cylinder is fully home position.
retracted to the home
position.
Tube Forward, S14 Optical Senses a specimen 1
See Figure 2.9-2 tube is present and WARNING Biohazardous contamination
pushed against the could occur from contact with the
stop (dead plate) in the needle and its associated tubing if not
Piercing station, ready handled with care. Wear protective gear
for piercing. and avoid skin contact.
1. Attach the safety clip to the needle

cartridge and remove the cartridge.
2. Manually push on the dead plate.
Tube Available, S7 Optical Senses the presence of 0 Press the sensor actuator; it is located in
See Figure 2.9-1 a specimen tube in the the center of the rocker bed.
cassette at the piercing
station.
Load Elevator Down, S8 Optical Senses when the right 0 Manually rotate the rocker bed to the rear
See Figure 2.9-1 elevator platform is all position, then carefully raise the load
the way down. elevator by gently pushing the
mechanism.
Unload Elevator Down, S6 Optical Senses when the left 0 Manually rotate the rocker bed to the rear
See Figure 2.9-1 elevator platform is all position, then carefully raise the unload
the way down. elevator by gently pushing the
mechanism.
Full Cass Index Rotation, S9 Optical Senses when the 1 Manually rotate the rocker bed to the rear
See Figure 2.9-1 cassette advance position, then carefully rotate the
mechanism is inactive. cassette index mechanism.
This sensor becomes
not true when the
cassette indexing
mechanism is
advancing the cassette.
Pierce Position, S16 Optical Senses when the 0 Manually rotate the rocker bed to the
See Figure 2.9-2 rocker bed is in the front (pierce) position.
forward, piercing
position.
Horizontal Position, S17 Optical Senses when the 1 Manually rotate the rocker bed from front
See Figure 2.9-2 rocker bed is in the to rear. The sensor should go "high"
level, cassette loading when the rocker bed is in the horizontal
and unloading position. position.
PN 4235961E 4.43-3
SWITCH/SENSOR CHECK

Cassette position 0, S12 Optical Senses a cassette is 0 Manually activate the sensor actuator; it
See Figure 2.9-1 placed on the rocker is located on the rear edge of the rocker
bed and is oriented bed, the first sensor actuator in from the
correctly, cassette right.
position 0.
Cassette Position 1, S2 Optical Senses a cassette has 0 Manually activate the sensor actuator; it
See Figure 2.9-1 advanced the first time, is located on the rear edge of the rocker
cassette position 1. bed, the second sensor actuator in from
the right.
Cassette Position 2, S3 Optical Senses that a cassette 0 Manually activate the sensor actuator; it
See Figure 2.9-1 is overlapping the is located on the rear edge of the rocker
elevator platform, bed, the third sensor actuator in from the
cassette position 2. right.
Cassette Position 3, S13 Optical Senses that a cassette 0 Manually activate the sensor actuator; it
See Figure 2.9-1 is over the elevator is located on the rear edge of the rocker
platform, cassette bed, the fourth sensor actuator in from
position 3. the right (or the first one in from the left).
4.43-4 PN 4235961E
Table 4.43-2 Switch/Sensor Checks on the MAXM Analyzer with Rotary Cap-Pierce Module

Whole Blood, S2-whole blood 0 Press the sample bar.
Backwash Safety, S3-backwash 0 Manually move the rinse block down to
the end of travel.
Rinse Block Arm, S4 1 Manually move rinse block assembly
Diluent, FD6 Float or Senses when the 1 Manually move the float sensor or
See Figure A.5-7 Optical diluent container is remove diluent from the optical chamber.
empty.
Cleaner, FD7 Float or Senses when the 1 Manually move the float sensor or
See Figure A.5-7 Optical cleaning agent remove cleaning agent from the optical
container is empty. chamber.
Waste Float Senses when the waste 1
container is full. WARNING Biohazardous contamination
could occur from contact with the waste
container and its associated tubing if
not handled with care. Wear protective
gear. Avoid skin contact. Clean up spills
immediately.
Manually move the float sensor.

Sheath Tank, S5-tank Float Senses when the 1 Manually drain the sheath tank, allowing
sheath tank is empty. the float sensor to drop to its lower end
of travel.
Needle Home, U1 Optical or Senses the needle is 0
See Figure 2.8-1, next to the Hall fully retracted to the WARNING Biohazardous contamination
needle assembly (optical) or effect home position. could occur from contact with the
on CL5 (Hall effect). needle assembly if not handled with
care. Wear protective gear and avoid
skin contact.

manually move the needle assembly
Needle Forward, U2 Optical or Senses when the 1
See Figure 2.8-1, next to the Hall needle is extended. WARNING Biohazardous contamination
needle assembly (optical) or effect could occur from contact with the
on CL5 (Hall effect). needle assembly if not handled with
skin contact.

manually move the needle assembly to
the fully forward position.
PN 4235961E 4.43-5
SWITCH/SENSOR CHECK
Table 4.43-2 Switch/Sensor Checks on the MAXM Analyzer with Rotary Cap-Pierce Module (Continued)

Normal Position, U3 Optical Senses a normal sized 1
See Figure 2.8-1 specimen tube is in the WARNING Biohazardous contamination
pierce position. could occur from contact with the
carousel assembly if not handled with
skin contact.
Manually rotate the carousel from the

normal size position.
Oversized Pos., U4 Optical Senses an oversized 0
See Figure 2.8-1 specimen tube is in the WARNING Biohazardous contamination
pierce position. could occur from contact with the
carousel assembly if not handled with
skin contact.
Manually rotate the carousel to the

oversize position.
Hand Detector, DS1, Q2 (LEDs) Infrared This two-part sensor, 0 Manually block the light path between
See Figure 2.8-1 DS1 transmit (on the the hand detector transmitter and
right) and Q2 receive receiver (the lower pair of LED sensors
(on the left), senses mounted on the carousel bracket).
when a tube is placed
in the carousel and
checks for objects
blocking the carousel
rotation path before the
carousel is rotated.
Door Interlock, S18 Magnetic Senses the front door 1 Open the front door or manually activate
is opened/closed. the sensor with an external magnet.
Barcode Detector, DS2, Q1 Infrared This two part sensor, 0 Manually block the light path between
(LEDs) DS2 transmit (on the the bar-code detector transmitter and
See Figure 2.8-1 left) and Q2 receive (on receiver (the upper pair of LED sensors
the right), senses the mounted on the carousel bracket).
presence of a tube and
activates the bar-code
laser scanner.
Tube Available, U5 Infrared Detects when a 0 Manually activate the tube sensor pivot
See Figure 2.8-1 specimen tube is in one arm assembly.
of the tube slots of the
carousel.
4.43-6 PN 4235961E
NEEDLE-POSITION SENSORS ADJUSTMENT 4
4.44 NEEDLE-POSITION SENSORS ADJUSTMENT
Purpose
Use this procedure to adjust the Hall effect needle-position sensors (needle-home or
needle-forward) on a Rotary Cap-Pierce module or an Autoloader module whenever you
replace:
r A needle-drive cylinder, CL5 (Rotary Cap-Pierce module) or CL6 (Autoloader module).

r A needle-home sensor, U1 (Rotary Cap-Pierce module) or S0 (Autoloader module).
r A needle-forward sensor, U2 (Rotary Cap-Pierce module) or S1 (Autoloader module).
Preliminary Check
ATTENTION: To make the description of the sensor to cylinder orientation universal, it is based
on the example shown in Figure 4.44-1.
Figure 4.44-1 Needle-Position Sensor Orientation on the Needle Drive Cylinder
Needle-drive 1.5 mm
cylinder Needle area (1/16 in.)
Top
LED
Needle-forward
sensor
M A
E R
A S
H A
IN
T
J U R Y : U S E S A F E T Y C L IP
S Y . D IS P O S E O F P R O P -
L Y . R E F E R T O P R O D U C T
N D L E O R S T O R E N E E D L E
O IN S T A L L . R E M O V E ,
N U A L S .
Setscrew
Setscrew
Needle-home
sensor
LED
5961300E
Bottom
1. Using Figure 4.44-1 as a reference, check the location and orientation of both needle-
position sensors on the needle-drive cylinder.
a. Verify that the needle-home sensor (U1 or S0) is mounted on the left side of the
cylinder (below the pressure tubing), that its LED faces away from the needle area,
and that the LED end of the sensor is flush with the bottom of the cylinder.
b. Verify that the needle-forward sensor (U2 or S1) is mounted on the right side of the
cylinder, that its LED faces toward the needle area, and that the LED end of the
sensor extends about 1.5 mm (1/16 in.) past the top of the cylinder.
PN 4235961E 4.44-1
NEEDLE-POSITION SENSORS ADJUSTMENT
CAUTION Overtightening the setscrew on a Hall effect sensor can damage the setscrew if the setscrew is
plastic, or damage the needle-drive cylinder if the setscrew is metal. Do not overtighten the setscrew on a
Hall effect sensor.
WARNING Risk of personal injury or contamination. The needle is very sharp and can contain biohazardous
material. To avoid skin puncture, use care when working in the Piercing station.
2. If a needle-position sensor is not positioned correctly on the needle-drive cylinder:

a. Power down the instrument as directed in Power Down/Power Up the System under
b. Loosen the setscrew for the sensor (Figure 4.44-1).
c. Reposition the sensor.
d. Tighten the setscrew to hold the sensor in place, but do not overtighten it.
e. Power up the instrument.
Adjustment
1. Check the position of the needle-home sensor:
a. Verify the needle is fully retracted.
b. With the needle fully retracted, verify the needle-home sensor’s LED is lit.
c. If the LED is not lit:
1) Loosen the setscrew for the needle-home sensor (Figure 4.44-1).
2) Move the sensor until the LED lights.
3) Tighten the setscrew to hold the sensor in place, but do not overtighten it.
2. On instruments with a Rotary Cap-Pierce module, check the position of the
needle-forward sensor as follows:
a. Remove the pressure tubing from the top fitting on the cylinder.
b. Rotate the carousel to the piercing position.
c. Manually push the needle driver assembly to fully extend the needle.
d. With the needle fully extended, verify the needle-forward sensors’s LED is lit.
e. If the LED is not lit:
1) Loosen the setscrew for the needle-forward sensor (Figure 4.44-1).
f. Push the needle back to the home position and reconnect the tubing to the needle-
drive cylinder.
3. On instruments with an Autoloader module, check the position of the needle-forward
sensor as follows:
a. Remove the pressure tubing from the top fitting on the cylinder.
b. Move the rocker bed to the forward position.
c. Manually push the needle cartridge driver to fully extend the needle.
4.44-2 PN 4235961E
NEEDLE-POSITION SENSORS ADJUSTMENT 4
d. With the needle fully extended, verify the needle forward-sensor’s LED is lit.
e. If the LED is not lit:
1) Loosen the setscrew for the needle forward sensor (Figure 4.44-1).
f. Push the needle back to the home position and reconnect the tubing to the needle-
drive cylinder.
Verification
3. Do a Start Up and verify all startup results Pass.
4. Check reproducibility in the Primary mode as directed under Heading 4.5,
REPRODUCIBILITY CHECKS.
5. Verify no needle-position errors were posted when cycling in the Primary mode.
6. Run controls and verify that the results fall within the expected range.
PN 4235961E 4.44-3
NEEDLE-POSITION SENSORS ADJUSTMENT
4.44-4 PN 4235961E
CONTENTS
5
5 MAINTENANCE PROCEDURES, 5.1-1
5.1 MAINTENANCE AND SYSTEM VERIFICATION RECOMMENDATIONS, 5.1-1

Required Service Forms, 5.1-1
Maintenance Schedule, 5.1-1
Verifying Instrument Performance, 5.1-1
Purpose, 5.1-1
Procedure, 5.1-1
Recording Reference Voltages, 5.1-1
PN 4235961E 5-i
CONTENTS
5-ii PN 4235961E
5MAINTENANCE PROCEDURES 5
5.1 MAINTENANCE AND SYSTEM VERIFICATION RECOMMENDATIONS
Required Service Forms
A copy of the following forms is included at the end of this chapter for your convenience.
Make copies as needed.
r COULTER® SYSTEM VERIFICATION FORM, PN 4276605

r COULTER® MAXM Instrument Reference Form, PN 4276421
r RECOMMENDED MAXM INSTRUMENT MAINTENANCE SCHEDULE, PN 4276419
Maintenance Schedule
The recommended schedule for component replacement is outlined in the RECOMMENDED
MAXM INSTRUMENT MAINTENANCE SCHEDULE, PN 4276419.
Verifying Instrument Performance
Purpose
Doing the System Verification Procedure (SVP) at the end of a service call ensures the
instrument is working correctly before you leave.
IMPORTANT Blood samples must be fresh, unrefrigerated, human blood obtained from normal donors and
drawn into EDTA tubes within the previous 4 hours.
B Whole blood
B COULTER® SYSTEM VERIFICATION FORM, PN 4276605
Procedure
Do the SVP and complete the SVP form at each service call. The minimum verification
requirements for any type of service performed on the MAXM analyzer is to do a startup, run
a reproducibility in the mode or modes used by the customer, and run controls.
On the back of the SVP form is a check list of all the procedures outlined in the MAXM
Analyzer Service Manual. Space is also provided on the back of the SVP form to record
procedures not on the check list.
Provide a completed copy of the SVP form to the customer for future reference.
Recording Reference Voltages

If the procedures you performed during a service call affected the reference voltages, you
must also fill in a copy of the COULTER® MAXM Instrument Reference Form, PN 4276421,
and leave it with the customer.
PN 4235961E 5.1-1
MAINTENANCE PROCEDURES
MAINTENANCE AND SYSTEM VERIFICATION RECOMMENDATIONS
5.1-2 PN 4235961E
®
COULTER HEMATOLOGY SYSTEM VERIFICATION FORM
Institution Name Date
Address Cycle Count
Model / Serial # Tracking #
Problem / Symptom
Corrective Action
SM's, (Service Mod's) Performed
On all types of maintenance completed, Perform:
ALL MODELS
Background Test Passed Verify against performance specification*
Reproducibility Run Passed Verify against performance specification*
Control Run Passed Verify against performance specification*
STKS™ Analyzer & MAXM™ Analyzer ONLY

BSV and BSV Housing Verification performed ‡
MAXM™ Analyzer ONLY

30 & 60 psi checks performed ‡
Additional comments
Customer Signature
Performed by
* Reference the Operators Manual for the appropriate instrument to obtain the correct Performance Specification.
‡ Reference the Service Manual for the appropriate instrument to obtain the Procedure.
4276605 Rev. A
Checked Box Indicates Function Performed
--------- ---------------- ------------------- --------
------------------ ----- ---------- ------------- ---
PROCEDURE / VERIFICATION PROCEDURE / VERIFICATION
STKS™ Analyzer
STKR Analyzer
MAXM™ Analyzer
S+ Analyzer Series
VCS Analyzer
MD / MD II Analyzer
ONYX Analyzer
JT / Jr. Analyzer
T Analyzer Series
JS/JT 2 & 3 Analyzer
HmX Analyzer
AcT Analyzer Series
Act Diff 2™ Analyzer
STKS™ Analyzer
STKR Analyzer
MAXM™ Analyzer
S+ Analyzer Series
VCS Analyzer
MD / MD II Analyzer
ONYX Analyzer
JT / Jr.Analyzer
T AnalyzerSeries
JS / JT 2 & 3 Analyzer
HmX Analyzer
AcT Analyzer Series
Act Diff 2™ Analyzer
PERFORMANCE CALIBRATION
Startup Tests CBC Latex Calibration
Background Count Aperture Balancing
CBC - CBC / Diff Reproducibility Flow Rate Matching
Retic Reproducibility Diff Latex Calibration, (Latron)
Carryover CBC - CBC/Diff Initial Adjustment to Control
Retic Mode to Mode & Within Mode Carryover Adjusted/Verified Clog Detection Factors
Mode to Mode Matching TTM
Verified Control Recovery Verified Diff / Retic Voltages and Noise Limits
ANALYZER / DILUTER Flow Cell Cleaning Procedure
Electronic Reference Tests Lens Block Disassembly / Cleaning
Analog Pulse Test TTM Alignment Procedure
Replaced / Cleaned / Verified BSV / MPCV VCS Flow Rate Adjustment
Replaced Pinch Valve / Actuator Clog Detector Circuit Adjustment
Replaced Tubing VCS Optimization Procedure
Replaced / Verified Peristaltic Pump Tubing PUMP VOLUME ADJUSTMENT / VERIFICATION
Replaced Cylinder CBC Lytic reagent
Replaced / Verified Switch / Sensor Erythrolyse / Stabilyse
Resistance Test (ground loop test) RBC Diluent
Replaced / Verified Solenoid operation WBC Diluent
Replaced / Verified Bar Code Reader Primary Aspiration
Blood Detector adjustment / Verification Secondary Aspiration
Diluent Lytic reagent dispense timing Checked Syringe Seals
Elevator removal/replacement Performed Pump Correction Factor Procedure
Hemoglobin lamp adjustment/replacement ELECTRONIC / PNEUMATIC SUPPLY
Manual aspiration rinse trough/probe adj. Checked Supply Voltages
Replaced / Cleaned Filter Checked Supply Pressures
Replaced / Verified Lamp / Indicator Checked Supply Vacuum
Replaced / Cleaned Bath(s) Replaced / Cleaned Filters
Replaced / Cleaned / Verified Aperture(s) Cleaned Vacuum / Foam Trap
Replaced / Adjusted / Verified Reagent Sensor Cleaned/Serviced Air / Water Separator / Purge Sol.
Replaced Sample / Diluent Syringe AUTOMATIC SAMPLE HANDLING SYSTEM
PRINTERS Replaced/Verified/ Switch/Sensor and Alignment
Verified graphics printer operation Performed Bar-Code Scanner Alignment
Verified ticket printer operation Performed Rocker Bed Linkage Adjustment
COMPUTER / DATA TERMINAL / DISPLAY / CPU CARD Sample tube stopper pierce-proximity/centering adj.
Verified Configuration / Initialization Adjusted cassette index motor/index hub gap
Replaced / Formatted / Partitioned Hard Drive Tube detector replacement/adjustment & verification
Installed Software Air cylinder replacement / verification
Verified / Modified CMOS Setup Rockerbed belt tension adjustment
Checked for Viruses CTPSA Verification Procedure
Replaced / Cleaned Floppy Disk Drive Replaced / Inspected tubing
Replaced / Cleaned / Verified Keyboard Replaced / Inspected pinch valve-(s)
Replaced/Cleaned/Verified Keypad/Touchscreen Cleaned / replaced needle
Replaced / Cleaned Filters Replaced / Inspected needle bellows/Rinse trough/block
Cleaned Monitor Screen / Display Other
Other Other
4276605 Rev. A
®
COULTER MAXM Instrument Reference Form
1. System Test
Affix (Staple) System Test printout to this document.
2. Calibration Factors
Primary Mode Secondary Mode
WBC WBC
RBC RBC
HGB HGB
MCV MCV
PLT PLT
MPV MPV
3. RMS Noise and Light Scatter Offset Voltages
CBC RMS Noise

Static
WBC
PLT
RBC
Diff RMS Noise Retic RMS Noise

Static Dynamic Static Dynamic
V
C
S
Light Scatter Offset Light Scatter Offset
DMS Software Revision:
Date: Model Code: Serial Number:
4276421 Rev. A (March 1997)

RECOMMENDED MAXM INSTRUMENT MAINTENANCE SCHEDULE
This schedule for component replacement is recommended only and may vary with instrument
usage and environment.
Every 12 Months, Every 20,000 Cycles or as Needed
√ Replace Aperture O-Rings

√ Replace the lower sheath restrictor
√ Replace the 6" sample line from flow cell to mixing chamber
√ Replace the mixing chamber
√ Replace the Quench line to the mixing chamber
√ Replace all pinch tubing
√ Replace the aspiration tubing from needle to blood detector
√ Replace the 4.5" rinse line from peltier to BSV wm-6
√ Replace the 4.0" rinse line from peltier to mixing chamber
√ Replace the 11.0" Sample line from BSV to mixing chamber
√ Replace the 7.6" CBC lytic reagent restrictor tubing.
√ Replace Aperture Optic Lamps as a set
Every 36 Months or as Needed
√ Replace waste and drain tubing

√ Replace diluent and cleaner tubing
√ Replace lytic reagent tubing
PN 4276419 Rev. B (November 1997)

CONTENTS
6
6 SCHEMATICS, 6.1-1
6.1 ENGINEERING SCHEMATICS, 6.1-1

Schematics Included, 6.1-1
Schematics Not Included but Available, 6.1-1
TABLES
6.1-1 Schematics Included in This Manual, 6.1-1
6.1-2 Schematics Available for the MAXM Analyzer but Not Included in This
Manual, 6.1-1
PN 4235961E 6-i
CONTENTS
6-ii PN 4235961E
6SCHEMATICS 6
6.1 ENGINEERING SCHEMATICS
This chapter contains the pneumatic/hydraulic and electronic schematics you need for
troubleshooting the MAXM analyzer. The schematic revision levels were current on the date
this revision of the manual was released. They will be updated again to the latest revision
level whenever this manual is revised.
Because the engineering schematics are simply inserted into the document and are not
assigned page numbers or figure numbers, they are not included in the table of contents or
index. In the text they are referenced by their name and document control number (DCN).
Schematics Included
Table 6.1-1 lists the schematics included in this manual, in the order in which they appear in
the printed manual. In the online manual the DCN numbers in Table 6.1-1 are linked to the
schematics.
Table 6.1-1 Schematics Included in This Manual
Name DCN
Pneu/Hydr Layout 6320510

Interconnect Diagram MAXM/HmX Systems 6320619
Pneumatic Power Supply, 100/120 Vac 6320683
Pneumatic Power Supply, 220/240 Vac 6320682
Power Supply Module 100/120 Vac 6320500
Power Supply Module 220/240 Vac 6320750
Schematics Not Included but Available

The schematics listed in Table 6.1-2 are not included in this manual, but they are available
and may be useful for in-depth troubleshooting of the MAXM analyzer.
Table 6.1-2 Schematics Available for the MAXM Analyzer but Not Included in This Manual
Name DCN
Laser Assembly, 100/120 V 6320611
Laser Assembly, 220/240 V 6320612
Backplane DIN96 6320400
Sample Handler 6320420
Sample Handler 2 6320895
Red/Wht Preamp + 6320414
376CPU 6320390
R/W/P Processor 6320364
Pneumatic Monitors 6320402
I/O 6320371
Diluter Interface 6320383
VCS Processor 6320499
PN 4235961E 6.1-1
SCHEMATICS
ENGINEERING SCHEMATICS
Table 6.1-2 Schematics Available for the MAXM Analyzer but Not Included in This Manual (Continued)
Name DCN
FL Det/RAM Press 6321202
Aperture, Voltage/Power Fail Detector 6320453
Peltier Temp Control 6320413
Autoloader Housing Interface Card 6320759
Autoloader Rocker Bed Interface Card 6320760
6.1-2 PN 4235961E
CONTENTS
7
7 TROUBLESHOOTING, 7.1-1
7.1 ERROR MESSAGES, 7.1-1
7.2 FLOW-CELL ERRORS FC, PC1, AND PC2, 7.2-1
7.3 DC, RF, OR LS NOISE PROBLEMS, 7.3-1

Purpose, 7.3-1
DC Noise Troubleshooting Checks, 7.3-1
RF Noise Troubleshooting Checks, 7.3-2
LS Noise Troubleshooting Checks, 7.3-7
7.4 DC, RF, OR LS LATEX CALIBRATION PROBLEMS, 7.4-1
7.5 REPORTED INSTRUMENT PROBLEMS AND SOLUTIONS, 7.5-1
ILLUSTRATIONS
7.3-1 Acceptable DC Noise Display, 7.3-1
7.3-2 Unacceptable DC Noise Display, 7.3-1
7.3-3 Acceptable Static RF Noise Display, 7.3-2
7.3-4 Unacceptable Static RF Noise Display, 7.3-2
7.3-5 Acceptable Dynamic RF Noise, 7.3-3
7.3-6 RF 60 Hz, 7.3-4
7.3-7 RF 60 Hz with Noise Spikes, 7.3-4
7.3-8 Normal RF Random Baseline Bounce, 7.3-4
7.3-9 RF Output with bubble Trapped in Sheath 2, 7.3-5
7.3-10 Normal RF Baseline While Cycling Diluent, 7.3-5
7.3-11 Noisy RF Baseline While Cycling Diluent, 7.3-5
7.3-12 Noisy RF Baseline While Cycling Diluent (RF Noise >100 mV), 7.3-6
7.3-13 LATRON Control Sample with a Good RF Tube and Box, 7.3-6
7.3-14 LATRON Control Sample with a Bad RF Tube and Box, 7.3-6
7.3-15 RF Output Running LATRON Control (Leak at QD7), 7.3-7
7.3-16 LATRON Control Run with DC Gain Drop, 7.3-7
7.3-17 Acceptable Static LS Noise Display, 7.3-8
7.3-18 Unacceptable Static LS Noise Display, 7.3-8
7.3-19 Acceptable Dynamic LS Noise Display, 7.3-9
TABLES
7.1-1 System Error Messages - Symbols and Numbers, 7.1-1
7.1-2 System Error Messages - A and B, 7.1-5
7.1-3 System Error Messages - C, 7.1-7
7.1-4 System Error Messages - D through H, 7.1-13
7.1-5 System Error Messages - I, 7.1-16
7.1-6 System Error Messages - J through P, 7.1-19
7.1-7 System Error Messages - R, 7.1-22
7.1-8 System Error Messages - S, 7.1-24
7.1-9 System Error Messages - T, 7.1-27
7.1-10 System Error Messages - U, 7.1-28
7.1-11 System Error Messages - V through Z, 7.1-30
7.1-12 Internal Instrument Codes, 7.1-32
PN 4235961E 7-i
CONTENTS
7.2-1 Flow-Cell Errors, 7.2-1
7.3-1 RF Troubleshooting Table, 7.3-3
7.4-1 RF, DC, or LS Latex Calibration Troubleshooting Table, 7.4-1
7.5-1 Troubleshooting Reported Instrument Problems, 7.5-1
7-ii PN 4235961E
7TROUBLESHOOTING 7
7.1 ERROR MESSAGES
Tables 7.1-1 through 7.1-11 list the error messages displayed on the DMS for the Analyzer
module, the Sample Handler, and the DMS, in alphabetic order. These tables also list the
action message displayed, when applicable, and the probable causes and corrective actions for
the error.
The error messages are listed in the instrument’s Error File. Press Ý+Ê to access the Error
File. It contains up to 100 error messages. When the Error File contains more than 100
messages, it overwrites the oldest message as a new message is posted. Use as needed for
troubleshooting.
All the error messages are referenced in the index.
Table 7.1-1 System Error Messages - Symbols and Numbers
Error Message Probable Cause Action Message Corrective Action

3 CONSECUTIVE Three consecutive flow cell PURGE THE 1. Clear the flow-cell clog. See the
FLOWCELL CLOGS clogs (any combination of FC, FLOWCELL customer’s Special Procedures and
PC1, and PC2) occurred. Troubleshooting manual.
2. To resume operation, access the
appropriate screen, reselect the
mode of operation, and rerun the
samples.
3 CONSECUTIVE NO Processed sample's primary CLEAR ERROR/ 1. Delete one or more samples
MATCHES identifier did not match any CHECK WLST/ associated with error from worklist.
entry in preassigned worklist for RESTART 2. Restart the system.
3 consecutive cycles.
3 CONSECUTIVE NO Bar-code reader unable to read CLEAR ERRORS/ 1. Check labels for proper positioning.
READS barcode on tube three CHECK LABELS/ 2. Delete one or more samples
consecutive times. RESTART associated with error from worklist.
3. Restart the system.
3 CONSECUTIVE Three consecutive attempts to CHECK/CLEAN 1. Check the needle for clogs and
PARTIAL aspirate were unsuccessful. THE NEEDLE clean if necessary. See the
ASPIRATIONS customer’s Special Procedures and
2. If the needle is not clogged, see the
Troubleshooting section in the
customer’s Special Procedures and
Troubleshooting manual for
additional corrective actions.
3. Delete one or more samples
associated with error from worklist.
samples.
PN 4235961E 7.1-1
TROUBLESHOOTING
ERROR MESSAGES
Table 7.1-1 System Error Messages - Symbols and Numbers (Continued)

3 CONSECUTIVE Three consecutive total voteouts CHECK/CLEAN 1. Zap the apertures. See the
VOTEOUTS of a particular parameter THE APERTURES customer’s Special Procedures and
occurred. Troubleshooting manual.
2. If error recurs, check the mixing
bubbles. See the Troubleshooting
section in the customer’s Special
Procedures and Troubleshooting
manual for corrective actions.
3. If error recurs, bleach the
apertures. See the customer’s
Special Procedures and
4. Delete one or more samples
samples.
+5 Vdc OUT OF +5 Vdc instrument voltage out of PERFORM 1. Perform System Test. See the
RANGE [XX.XX] operating range. SYSTEM TEST customer’s Special Procedures and
Note: XX.XX = Range is +4.75 to +5.25 Vdc. Troubleshooting manual.
actual reading. 2. Replace fuse if necessary. See the
+6.3 Vdc OUT OF +6.3 Vdc instrument voltage out PERFORM 1. Perform System Test. See the
RANGE [XX.XX] of operating range. SYSTEM TEST customer’s Special Procedures and
10 NO READ, NO Ten attempts to read, match, CLR ERRORS/CHK 1. Delete one or more samples with
MATCH, PART.ASP and/or aspirate sample were LBL,WLST, ASP/ NO READ, NO MATCH or PART ASP
unsuccessful and posted on RESTART messages from worklist.
worklist. 2. Check labels for proper positioning
and needle for clogs.
3. Clean needle if necessary. See the
10-13 mm TUBE Error detected by tube sensor; CLEAN 1. Clean code wheel sensors. See the
SENSOR ERROR code wheel sensors should be CODEWHEEL customer’s Special Procedures and
Note: Applicable to cleaned or are faulty. SENSORS Troubleshooting manual.
MAXM analyzer 2. To resume operation, access the
with Rotary appropriate screen, reselect the
Cap-Pierce module mode of operation, and rerun the
only. samples.
7.1-2 PN 4235961E
TROUBLESHOOTING
ERROR MESSAGES 7

+12 Vdc OUT OF +12 Vdc instrument voltage out PERFORM 1. Perform System Test. See the
-15 Vdc OUT OF -15 Vdc instrument voltage out PERFORM 1. Perform System Test. See the
Note: XX.XX = Range is -15.75 to -14.25 Vdc. Troubleshooting manual.
16 mm TUBE Error detected by tube sensor; CLEAN 1. Clean code wheel sensors. See the
SENSOR ERROR code wheel sensors should be CODEWHEEL customer’s Special Procedures and
Note: Applicable to cleaned or are faulty. SENSORS Troubleshooting manual.
only. samples.
30 PSI OUT OF Pressure out of established CHECK/ADJUST 1. Perform System Test to check the
RANGE [XX.XX] operating range. 30 PSI pressure. See the customer’s
Note: XX.XX = Special Procedures and
actual reading. Troubleshooting manual.
2. Adjust the 30 psi pressure. See the
PN 4235961E 7.1-3
TROUBLESHOOTING
ERROR MESSAGES

Note: XX.XX = Range is +285 to +315 Vdc. Troubleshooting manual.
+1350 Vdc OUT OF +1350 Vdc instrument voltage PERFORM 1. Open the right side door. Check if
RANGE [XX.XX] out of operating range. SYSTEM TEST the red LED is lit on the TTM laser
Note: XX.XX = Range is +1186 to +1523 Vdc. cover. If it is not, press down on the
actual reading. cover. If this does not correct the
problem, go to step 2.
2. Perform System Test. See the
3. Replace fuse if necessary. See the
7.1-4 PN 4235961E
TROUBLESHOOTING
ERROR MESSAGES 7
Table 7.1-2 System Error Messages - A and B

A/D FAILURE Analog-to-digital converter failed RE-ENTER 1. Attempt to perform requested
to generate an interrupt within FUNCTION/ function again, and rerun the
specified time. RECYCLE specimen.
SPECIMEN 2. If error recurs, reset the system.
A/D MEASUREMENT Analog-to-digital converter RE-ENTER 1. Attempt to perform requested
ERROR encountered too much noise on FUNCTION/ function again, and rerun the
ground reference. RECYCLE specimen.
ANALYSIS NOT Instrument error detected by RESET THE Reset the system.
DONE CPU while sample analysis in SYSTEM
progress. System locked up.
ANALYZER POWER Instrument detected Analyzer DMS WILL RESET 1. Any process running in DMS
INTERRUPTION power failure. terminates, and the DMS resets.
The system resynchronizes and
returns to Ready state.
2. To resume operation, reselect the
desired function.
AUTOLOADER Autoloader module reached SELECT STOP Restart the Primary mode:
PAUSED maximum allowable rocking THEN START
1. From the Sample Analysis Run
Note: Applicable to time for cassettes on rocker bed. PRIMARY
window, press Ñ Stop.
MAXM analyzer Autoloader module paused to
prevent damage to samples in 2. Press Ê Start Primary to resume
with Autoloader
cassettes. System now in Autoloader module operation.
module only.
Secondary mode.
BACKWASH NOT Rinse block not in proper PERFORM RINSE From the Sample Analysis Run window,
PERFORMED position for backwash. press Ð Rinse.
BAD DOWNLOAD DMS received bad download RESET THE Reset the system.
MSG RCVD - 196 message while downloading 196 SYSTEM
CODE code. System locked up.
BAD PORT IN USE TO Data sent to wrong port. System RESET THE Reset the system.
SEND DATA locked up. SYSTEM
BARCODE NOT READ Barcode reader unable to read CHECK LABEL Verify the bar-code label is properly
barcode on tube label. QUALITY positioned on the tube and has correct
specifications.
BARCODE READER Bar-code scanner not active. RESET THE Reset the system.
DID NOT RESPOND Cass/pos and tube labels not SYSTEM
Note: Applicable to read. System locked up.
MAXM analyzer
with Autoloader
module only.
PN 4235961E 7.1-5
TROUBLESHOOTING
ERROR MESSAGES
Table 7.1-2 System Error Messages - A and B (Continued)

BARCODE WAND NO Bar-code wand could not read RETRY Check the bar-code label for proper
READ bar-code label. positioning and rescan the label.
BLOOD Instrument unable to detect REMIX AND 1. Remix the blood specimen and
COMPARISON OUT presence of blood. REPEAT THE rerun it.
OF LIMITS SAMPLE 2. If error recurs, run in the
Secondary mode.
7.1-6 PN 4235961E
TROUBLESHOOTING
ERROR MESSAGES 7
Table 7.1-3 System Error Messages - C

CAL FACTORS NOT At least one calibration factor REPEAT CBC Check the limits and recalibrate the
WITHIN LIMITS requested for transmission is CALIBRATION instrument.
not within specified limits.
CANNOT BATCH. Attempt made to batch transmit PRESS F9-STOP. 1. Wait until the sample is completed.
SYSTEM IN RUN or print while system was RETRY BATCH 2. From the Run window, press
MODE processing a sample in Run XMIT/PRN Ñ Stop (to set DMS mode to no
mode. activity) and request batch
processing again.
CANNOT CYCLE Attempt made to run a sample WAIT/STOP Wait until batch processing is
WHILE BATCH while batch processing in PROCESSING TO completed, then attempt to run sample
PROCESSING progress. START CYCLE or cancel batch (Special Functions tt
Set Up tt Sample Analysis tt Set Up tt
Delete Host Spooler or Clear Printer
Spooler Queue) process.
CANNOT MOVE Cassette does not move along CHECK BED 1. Put the instrument in Standby.
CASSETTE ON BED the bed; cassette jammed, MECHANISM/ 2. Remove the jammed cassette.
Note: Applicable to indexing mechanism failed, or CASSETTE
3. Verify performance of indexing
MAXM analyzer cassette motor faulty.
mechanism. See the customer’s
with Autoloader Special Procedures and
module only. Troubleshooting manual.
CANNOT OPEN System unable to open DISK MAY BE Call your Beckman Coulter
RAW.DAT FILE RAW.DAT file. Space on hard FULL Representative.
disk may be insufficient. Service only: Verify amount of space
remaining. If necessary, recover hard
disk space from options previously
selected by accessing and clearing
control files or by removing data.
CANNOT ROCK BED Bed does not rock; indexing CHECK FOR Verify performance of the indexing
Note: Applicable to mechanism jammed or rocking JAMMED mechanism. See the customer’s Special
MAXM analyzer motor faulty. MECHANISM Procedures and Troubleshooting manual.
with Autoloader
module only.
PN 4235961E 7.1-7
TROUBLESHOOTING
ERROR MESSAGES
Table 7.1-3 System Error Messages - C (Continued)

CANNOT STORE RET 5C cell control label detected REANALYZE IN To run 5C cell control sample, change
RESULTS IN 5C while current sample mode set CORRECT CYCLE cycle type to CBC+Diff.
FILE to RETIC. TYPE
CANNOT TRANSMIT Attempt made to transmit MUST STOP 1. From the Sample Analysis Run
CAL FACTORS calibration factors from Primary INSTRUMENT screen, press Ñ Stop.
or Secondary CBC Calibration 2. Attempt to transmit calibration
screen while the system was factors.
running sample.
CAROUSEL DID NOT Carousel does not rotate; CHECK FOR A 1. Put the instrument in Standby.
ROTATE indexing mechanism jammed or JAMMED 2. Open the lower front cover and
Note: Applicable to rotation motor faulty. MECHANISM manually rotate the carousel.
MAXM analyzer Remove the tube if present.
with Rotary 3. If the carousel is jammed or
Cap-Pierce module binding, call your Beckman Coulter
only. Representative.
4. Close the lower front cover and if
the problem is resolved, return the
Standby/Ready switch to READY.
CARRYOVER IS Attempt made to change TO CHANGE From the Sample Analysis Run screen,
ACTIVE carryover mode of operation MODE, PRESS press Ñ Stop to change mode of
without data in carryover file and F9-STOP operation.
carryover sample mode was
active.
CASSETTE LABEL NO Single NO READ of Cass/pos RESTART, 1. Check the cassette label for proper
READ number occurred, or cassette POSITION WILL positioning.
Note: Applicable to not advanced. BE SKIPPED 2. Access the appropriate screen and
MAXM analyzer reselect the mode of operation.
with Autoloader
module only.
CASSETTE LOAD Cassette does not load; cassette CHECK FOR Remove the jammed cassette. See the
FAILURE jammed in loading bay. JAMMED customer’s Special Procedures and
Note: Applicable to CASSETTE Troubleshooting manual.
MAXM analyzer
with Autoloader
module only.
7.1-8 PN 4235961E
TROUBLESHOOTING
ERROR MESSAGES 7

CASSETTE UNLOAD Cassette does not unload; CHECK FOR Remove the jammed cassette. See the
FAILURE cassette jammed in unloading JAMMED customer’s Special Procedures and
Note: Applicable to bay. CASSETTE Troubleshooting manual.
MAXM analyzer
with Autoloader
module only.
CBC CALIBRATION CBC calibration completed 60 CLEAR TABLE Clear the table (delete 60 samples table)
TABLE FULL samples; attempt made to run CLEAR TABLE/ and reselect the appropriate mode of
another sample. RERUN TEST operation to rerun desired test.
CBC DATA CBC acquisition could not be RE-ENTER 1. Attempt to perform the requested
ACQUISITION completed because of pending FUNCTION/ function again, and rerun the
FAILURE error. RECYCLE specimen.
CLEANER OUT Out-of-cleaner signal detected REPLACE 1. Replace the cleaning agent.
from reagent sensor; insufficient CLEANER, 2. Update the reagent file.
cleaner to perform another UPDATE FILE,
3. Prime the cleaner.
cycle. PRIME
Service only: If the cleaning agent
container is not empty, check for -
r A damaged or improperly installed
pick-up tube.
r A loose or disconnected reagent
supply line.
r Bubbles trapped in a check valve.
COMMAND The system could not complete CHECK SYSTEM Verify the cables are plugged firmly into
COMPLETION NOT request from DMS. CABLES the correct jacks. See the Installation
SUCCESSFUL Chapter of the Reference manual.
COMMAND TO Digiboard software detected CHECK SYSTEM Verify the cables are plugged firmly into
DIGIBOARD NOT error and rejected command CABLES the correct jacks. See Installation
ACCEPTED from DMS. Chapter of the Reference manual.
COMPRESSOR DID Compressor bleed sequence CONTACT Call your Beckman Coulter
NOT BLEED activated, but pressure did not SERVICE Representative.
[XX.XX] drop in specified time.
Note: XX.XX =
actual reading.
PN 4235961E 7.1-9
TROUBLESHOOTING
ERROR MESSAGES

COMPRESSOR Compressor pressure did not PERFORM 1. Perform System Test to confirm the
PRESSURE ERROR rise to normal operating range. SYSTEM TEST pressure reading. See the
[XX.XX] customer’s Special Procedures and
Note: XX.XX = Troubleshooting manual.
actual reading. 2. If the compressor pressure is still
out of range, call your Beckman
Coulter Representative.
3. Service only: Adjust the pressure.
CONTROL SAMPLE Carryover sample mode active, BEGIN If sample identified as control by ID,
IDENTIFIED and 5C control run. Carryover CARRYOVER TEST press Ñ Stop, reselect the sample
test invalidated. AGAIN/QUIT mode, and restart the test.
CONTROL SAMPLE Diff mode off when 5C control DIFF OFF, DIFF Set Diff mode to ON and rerun the 5C
IDENTIFIED run; results cannot be reported. RESULTS control.
UNAVAILABLE
COULD NOT ACCESS Autoprint queue does not exist No action message 1. Reset the system.
AUTOPRINT or is in use. associated with 2. Reselect the function.
QUEUE this error.
3. If error recurs, call your Beckman
COULD NOT ACCESS Batch print queue does not exist No action message 1. Reset the system.
BATCH QUEUE or is in use. associated with 2. Reselect the function.
this error.
COULD NOT ACCESS Queue for manual print requests No action message 1. Reset the system.
MANUAL QUEUE does not exist or is in use. associated with 2. Reselect the function.
this error.
COULD NOT CLOSE Autoprint queue exists but No action message 1. Reset the system.
AUTOPRINT cannot be closed. associated with 2. Reselect the function.
QUEUE this error.
COULD NOT CLOSE Batch print queue exists but No action message 1. Reset the system.
BATCH QUEUE cannot be closed. associated with 2. Reselect the function.
this error.
COULD NOT CLOSE Queue for manual print requests No action message 1. Reset the system.
MANUAL QUEUE exists but cannot be closed. associated with 2. Reselect the function.
this error.
COULD NOT DELETE Autoprint file exists that is No action message 1. Reset the system.
AUTOPRINT DATA already spooled; therefore, file associated with 2. Reselect the function.
cannot be deleted. this error.
7.1-10 PN 4235961E
TROUBLESHOOTING
ERROR MESSAGES 7

COULD NOT DELETE Autoprint queue does not exist No action message 1. Reset the system.
AUTOPRINT or is in use; therefore, queue associated with 2. Reselect the function.
QUEUE cannot be deleted at this time. this error.
COULD NOT DELETE Batch print file exists that is No action message 1. Reset the system.
BATCH DATA already spooled; therefore, file associated with 2. Reselect the function.
cannot be deleted. this error.
COULD NOT DELETE Batch print queue does not exist No action message 1. Reset the system.
BATCH QUEUE or is in use; therefore, queue associated with 2. Reselect the function.
cannot be deleted at this time. this error.
COULD NOT DELETE File for manual print queue No action message 1. Reset the system.
MANUAL DATA exists that is already spooled; associated with 2. Reselect the function.
therefore, file cannot be deleted. this error.
COULD NOT DELETE Queue for manual print requests No action message 1. Reset the system.
MANUAL QUEUE does not exist or is in use; associated with 2. Reselect the function.
therefore, queue cannot be this error.
deleted at this time.
COULD NOT FIND Autoprint queue exists; however, No action message 1. Reset the system.
AUTOPRINT DATA there is no data in the queue. associated with 2. Reselect the function.
this error.
COULD NOT FIND Batch print queue exists; No action message 1. Reset the system.
BATCH DATA however, there is no data in the associated with 2. Reselect the function.
queue. this error.
COULD NOT FIND Queue for manual print requests No action message 1. Reset the system.
MANUAL DATA exists; however, there is no data associated with 2. Reselect the function.
in the queue. this error.
COULD NOT OPEN Autoprint queue exists; however, No action message 1. Reset the system.
AUTOPRINT it cannot be opened to be read associated with 2. Reselect function.
QUEUE from or written to. this error.
COULD NOT OPEN Batch print queue exists; No action message 1. Reset the system.
BATCH QUEUE however, it cannot be opened to associated with 2. Reselect the function.
be read from or written to. this error.
PN 4235961E 7.1-11
TROUBLESHOOTING
ERROR MESSAGES

COULD NOT OPEN Queue for manual print requests No action message 1. Reset the system.
MANUAL QUEUE exists; however, it cannot be associated with 2. Reselect the function.
opened to be read from or this error.
written to.
COULD NOT READ Autoprint queue exists and is No action message 1. Reset the system.
FROM AUTOPRINT open; however, it cannot be associated with 2. Reselect the function.
QUEUE read. this error.
COULD NOT READ Batch print queue exists and is No action message 1. Reset the system.
FROM BATCH open; however, it cannot be associated with 2. Reselect the function.
QUEUE read. this error.
COULD NOT READ Queue for manual print requests No action message 1. Reset the system.
FROM MANUAL exists and is open; however, it associated with 2. Reselect the function.
QUEUE cannot be read. this error.
COULD NOT WRITE Autoprint queue exists and is No action message 1. Reset the system.
TO AUTOPRINT open; however it cannot be associated with 2. Reselect the function.
QUEUE written to. this error.
COULD NOT WRITE Batch print queue exists and is No action message 1. Reset the system.
TO BATCH QUEUE open; however, it cannot be associated with 2. Reselect the function.
written to. this error.
COULD NOT WRITE Queue for manual print requests No action message 1. Reset the system.
TO MANUAL exists and is open; however, it associated with 2. Reselect the function.
QUEUE cannot be written to. this error.
CRC ERROR ON Cyclic redundancy check (CRC) RESET THE Reset the system.
READ SYSTEM.CFG error occurred while reading SYSTEM
FILE SYSTEM.CFG file. System
locked up.
CTL FILE I/O ERROR Control file input/output error RESET THE Reset the system.
occurred. System locked up. SYSTEM
CTL FILE NN, <FILE> Control file number NN, filename CLEAR THE 1. Delete data in control file NN.
IS FULL <FILE> full; no further data can CONTROL FILE 2. Reselect the mode of operation. If
be saved to file. mode is currently active, press
Ñ Stop to change to Stop mode.
3. Restart the test.
7.1-12 PN 4235961E
TROUBLESHOOTING
ERROR MESSAGES 7
Table 7.1-4 System Error Messages - D through H

DIFF DATA Differential data acquisition RE-ENTER Reselect the function, and recycle the
ACQUISITION could not be completed because FUNCTION/ specimen.
FAILURE of pending error. RECYCLE
SPECIMEN
DIFF PRESSURE OUT Differential pressure not within CHECK/ADJUST 1. Perform System Test to confirm the
OF RANGE established operating range. DIFF PRESSURE problem. See the customer’s
2. If the diff pressure is still out, call
your Beckman Coulter
Representative.
3. Service only: Adjust the differential
pressure.
DILUENT Diluent comparison out of limits PRIME DILUENT 1. Prime the diluent, and repeat the
COMPARISON OUT and could not detect presence of AND REPEAT THE sample.
OF LIMITS diluent. SAMPLE 2. If problem recurs, check that the
BSV is rotating completely. If it is
not, clean and cycle the BSV. See
the customer’s Special Procedures
and Troubleshooting manual.
DILUENT OUT Out-of-diluent signal detected REPLACE 1. Replace the diluent.
from reagent sensor; insufficient DILUENT, UPDATE 2. Update the reagent file.
diluent to perform another cycle. FILE, PRIME
3. Prime the diluent.
Service only: If the diluent container is
not empty, check for -
pick-up tube.
supply line.
DILUTER TABLE During download, instrument RESET THE Reset the system.
ERROR could not find usable diluter SYSTEM
table.
DISK DRIVE C: Drive C has less than one No action message Call your Beckman Coulter
COULD NOT BE megabyte of space left; associated with Representative.
ACCESSED therefore, new file for queue this error. Service only: Delete files on drive C and/
cannot be created in drive C. or recover hard disk space from options
previously selected by removing data.
PN 4235961E 7.1-13
TROUBLESHOOTING
ERROR MESSAGES
Table 7.1-4 System Error Messages - D through H (Continued)

DISK DRIVE C: IS Space on drive C hard disk CHECK Call your Beckman Coulter
FULL insufficient for execution of DATABASE/ Representative.
requested option. REMOVE DATA Service only: Recover drive C hard disk
space from options previously selected
by removing data.
DISK DRIVE D: IS Space on drive D hard disk CHECK/CLEAR Call your Beckman Coulter
FULL insufficient for execution of CONTROL FILES Representative.
requested option. Service only: Recover drive D hard disk
space from options previously selected
by accessing and clearing control files.
DISK FULL - Space on diskette insufficient for No action message 1. Remove the full diskette from the
ARCHIVING archiving all of the tagged associated with DMS diskette drive, and insert an
DISCONTINUED samples in the database. this error. empty formatted diskette.
2. Ensure the archive option selected
is New.
3. Press Ð Execute. Any samples
tagged but not archived are copied
onto the new diskette.
DMS TIMEOUT DMS did not respond to RESET THE Reset the system.
instrument request in allotted SYSTEM
time. The system locked up.
DOWNLOAD NOT Errors occurred during CHECK ERROR 1. Check the Error Log to determine
SUCCESSFUL download of software to LOG why the download was
instrument from DMS. unsuccessful.
2. Reset the system.
3. If problem recurs, call your
Beckman Coulter Representative.
ERROR FILE I/O Error file input/output error RESET THE Reset the system.
ERROR occurred on ERROR file. The SYSTEM
system locked up.
ERROR READING SYSTEM.CFG file could not be RESET THE Reset the system.
SYSTEM.CFG FILE read. The system locked up. SYSTEM
ERROR UPDATING SYSTEM.CFG file could not be RESET THE Reset the system.
SYSTEM.CFG FILE updated. The system locked up. SYSTEM
EXTENSIVE FLAGS Cell classification flags too PRINT FOR A Print the sample results to get a
GENERATED extensive to display on screen. COMPLETE complete list of generated flags.
LISTING
FILE I/O ERROR File input/output error occurred. RESET THE Reset the system.
The system locked up. SYSTEM
7.1-14 PN 4235961E
TROUBLESHOOTING
ERROR MESSAGES 7
Table 7.1-4 System Error Messages - D through H (Continued)

HGB OUT OF RANGE Hemoglobin lamp voltage not PERFORM 1. Perform System Test to confirm the
within established operating SYSTEM TEST problem. See the customer’s
range. Special Procedures and
Range is 6.65 to 7.35 V. Troubleshooting manual.
2. If the Hgb is still out of range:
a. Check the Hgb lamp and
replace it if it is out. See the
customer’s Special
Procedures and
b. Adjust the Hgb lamp. See the
customer’s Special
Procedures and
HIGH VACUUM OUT High vacuum not within CHECK/ADJUST 1. Check that the vacuum trap is not
OF RANGE established operating range. HIGH VACUUM full of liquid.
Range is 17.00 to 2. Check for any obviously split or
28.00 in. of Hg (at sea level). disconnected tubing.
3. Perform System Test to confirm the
high vacuum reading. See the
4. If the high vacuum is still out of
range, call your Beckman Coulter
Representative.
HOST COMM. Host communication parameters DEFINE AND SAVE Define the host parameters on the
PARAMETERS not defined. PARAMETERS Communication Definition screen, and
UNDEFINED save.
HOST TX BUFF DMS attempted to start new CHECK HOST Verify the host computer cables are
REQUEST NOT operation before previous CABLES plugged firmly into the correct jacks. See
SUCCESSFUL operation completed. Installation Chapter of the Reference
manual.
PN 4235961E 7.1-15
TROUBLESHOOTING
ERROR MESSAGES
Table 7.1-5 System Error Messages - I

ID CANCELED-TIME Sample ID number entered was REENTER PATIENT Re-enter the sample ID number, and
EXPIRED canceled because time to ID process sample.
respond to Cancel prompt
expired.
IDENTIFICATION Sample ID number must be RETURN TO 1. Return to the Sample Analysis
REQUIRED entered to process sample. SAMPLE screen, and enter sample ID
ANALYSIS, ENTER number.
ID 2. Process the sample.
ILLEGAL Illegal reply received from RESET THE Reset the system.
INSTRUMENT instrument. The system SYSTEM
REPLY locked up.
INCOMPATIBLE Instrument detected that No action message 1. Reset the system.
SAMPLE HANDLER downloaded software associated with 2. If problem recurs, call your
SOFTWARE incompatible with sample this error. Beckman Coulter Representative.
handler hardware. The system
3. Service only: Check code 196
locked up.
software type (i.e., Autoloader
module or Rotary-Cap Pierce
module) on DMS and, if necessary,
load appropriate software.
INCOMPLETE Needle did not fully retract from REFER TO See the Troubleshooting section in the
NEEDLE EXTRACT tube; needle jammed or sensor MANUAL FOR customer’s Special Procedures and
Note: Applicable to failed. HELP Troubleshooting manual for corrective
MAXM analyzer actions.
with Autoloader
module only.
INCOMPLETE Needle did not fully retract from REFER TO Call your Beckman Coulter
NEEDLE EXTRACT tube; needle jammed or sensor MANUAL FOR Representative.
Note: Applicable to failed. HELP
MAXM analyzer
with Rotary-Cap
Pierce module only.
INCOMPLETE Needle did not completely pierce REFER TO See the Troubleshooting section in the
NEEDLE PIERCE tube. MANUAL FOR Special Procedures and Troubleshooting
Note: Applicable to HELP manual for corrective actions.
MAXM analyzer
with Autoloader
module only.
INCOMPLETE Needle did not completely pierce REFER TO Call your Beckman Coulter
NEEDLE PIERCE tube. MANUAL FOR Representative.
Note: Applicable to HELP
MAXM analyzer
with Rotary-Cap
Pierce module only.
7.1-16 PN 4235961E
TROUBLESHOOTING
ERROR MESSAGES 7
Table 7.1-5 System Error Messages - I (Continued)

INCOMPLETE Needle did not fully retract after REFER TO See the Troubleshooting section in the
NEEDLE RETRACT piercing tube; needle jammed or MANUAL FOR Special Procedures and Troubleshooting
Note: Applicable to sensor failed. HELP manua for corrective actions.
MAXM analyzer
with Autoloader
module only.
INCOMPLETE Needle did not fully retract after REFER TO Call your Beckman Coulter
NEEDLE RETRACT piercing tube; needle jammed or MANUAL FOR Representative.
Note: Applicable to sensor failed. HELP
MAXM analyzer
with Rotary
Cap-Pierce module
only.
INCOMPLETE RAW Raw data could not be RESET THE Reset the system.
DATA completely transmitted because SYSTEM
TRANSMISSION size of RAW.DAT file inadequate.
The system locked up.
INCOMPLETE TUBE Tube did not move completely CHECK FOR A Remove the jammed tube. See the
FORWARD forward; tube jammed. JAMMED TUBE customer’s Special Procedures and
Note: Applicable to Troubleshooting manual.
MAXM analyzer
with Autoloader
module only.
INCOMPLETE TUBE Tube did not return to cassette CHECK FOR A Remove the jammed tube. See the
RETRACT from piercing position; tube JAMMED TUBE customer’s Special Procedures and
Note: Applicable to jammed. Troubleshooting manual.
MAXM analyzer
with Autoloader
module only.
INCONSISTENT Instrument detected that CHECK 1. Reset the system.
SAMPLE HANDLER downloaded software HARDWARE-REIN 2. If error recurs, call your Beckman
HARDWARE incompatible with sample STALL SOFTWARE Coulter Representative.
handler hardware. The system
3. Service only: Check code 196
locked up.
software type (that is Autoloader or
Cap Piercer) on DMS and, if
necessary, load appropriate
software.
INSTRUMENT Error detected in random access RESET THE Reset the system.
CONFIGURATION memory (RAM). The system SYSTEM
ERROR locked up.
PN 4235961E 7.1-17
TROUBLESHOOTING
ERROR MESSAGES
Table 7.1-5 System Error Messages - I (Continued)

INSTRUMENT Internal instrument error RESET THE Reset the system.
INTERNAL ERROR detected by CPU. The system SYSTEM
XXX locked up.
Note: XXX = actual For code definition, refer to
internal code Table 7.1-12, Internal
number. Instrument Codes.
INSTRUMENT REPLY Time expired while waiting for RESET THE Ensure that the lower front door is
TIMEOUT reply from instrument. The SYSTEM closed. Reset the system.
system locked up.
INSTRUMENT TO 196 code download failed from RESET THE Ensure that the lower front door is
196 CODE DWNLD instrument to sample handler. SYSTEM closed. Reset the system.
FAILED The system locked up.
INV LABEL - STORED Beckman Coulter label other USE VALID Acknowledge error by pressing Þ+ã
IN CURRENT MODE than 5C control bar-code ID label LABELS ONLY to stop beeping. Rerun sample with the
(that is, 4C Plus cell control or 5C cell control bar-code ID label.
S-CAL calibrator) detected.
INVALID 376 MOD/ During 196 download, DMS RESET THE Reset the system.
CMD RCVD 196 received response from SYSTEM
DWNLD instrument with incorrect
destination.
INVALID 376 MOD/ During 196 configuration RESET THE Reset the system.
CMD RCVD 196CFG download, DMS received SYSTEM
DLD response from instrument with
incorrect destination.
INVALID MOD/CMD During diluter download, DMS RESET THE Reset the system.
IN DLTR DWNLD received response from SYSTEM
instrument with incorrect
destination.
INVALID MOD/CMD During 376 configuration RESET THE Reset the system.
RCVD 376CFG download, DMS received SYSTEM
DWNLD response from instrument with
incorrect destination.
INVALID MOD/CMD During diluter download, DMS RESET THE Reset the system.
RCVD IN DLTR received response from SYSTEM
DWNLD instrument with incorrect
destination.
INVALID SYSTEM Sample handler detected invalid RESET THE Reset the system.
COMMAND command from instrument. The SYSTEM
system locked up.
7.1-18 PN 4235961E
TROUBLESHOOTING
ERROR MESSAGES 7
Table 7.1-6 System Error Messages - J through P

LESS THAN 1 Drive C has less than one No action message Call your Beckman Coulter
MEGABYTE LEFT megabyte of space. associated with Representative.
ON DRIVE C this error. Service only: Delete unnecessary files
from drive C.
LOAD ELEVATOR Load (right) elevator not CHECK ELEVATOR Verify performance of the elevator
FAILURE functioning. Load elevator’s MECHANISM mechanism. See the customer’s Special
Note: Applicable to motor may have failed. Procedures and Troubleshooting manual.
MAXM analyzer
with Autoloader
module only.
LOAD STACK NOT Loading bay not empty. This EMPTY THE LOAD 1. Empty the loading bay.
EMPTY prevents load (right) elevator STACK 2. To test performance of loading
Note: Applicable to from functioning properly. elevator, reselect desired function.
MAXM analyzer
with Autoloader
module only.
LOW VACUUM OUT Low vacuum out of established CHECK/ADJUST 1. Check that the vacuum trap is not
OF RANGE operating range. Range is 5.940 LOW VACUUM full of liquid.
to 6.060 in. of Hg (at sea level). 2. Check for any obviously split or
disconnected tubing.
3. Perform System Test to check the
vacuum. See the customer’s
4. Adjust the vacuum. See the
LOWER DOOR OPEN Door opened when not in Stop CLOSE DOOR/ Close door and reselect the desired
mode. RESELECT function.
FUNCTION
LYSE OUT Out-of-lyse signal detected from REPLACE LYSE, 1. Replace the lytic reagent.
reagent sensor; insufficient lytic UPDATE FILE, 2. Update the reagent file.
reagent to perform another PRIME
3. Prime the lyse.
cycle.
Service only: If the diluent container is
not empty, check for -
pick-up tube.
supply line.
MAXM TX BUFF DMS attempted to start new CHECK MAXM Verify the cables are plugged firmly into
REQUEST NOT operation before previous CABLES the correct jacks. See Installation
SUCCESSFUL operation completed. Chapter of the Reference manual.
PN 4235961E 7.1-19
TROUBLESHOOTING
ERROR MESSAGES
Table 7.1-6 System Error Messages - J through P (Continued)

MEMORY ERROR DMS memory error detected. RESET THE Reset the system.
The system locked up. SYSTEM
MULT. INTER. WHILE DMS detected communication RESET THE Reset the system.
RECEIVING DATA errors while receiving data. The SYSTEM
system locked up.
MULTIPLE ERRORS More than one error detected by CHECK ERROR Check Error Log (Ý+Ê) to see
instrument. LOG which errors occurred and the
appropriate corrective actions.
NEEDLE FORWARD Needle forward sensor may be REFER TO 1. Clean the needle forward sensor.
SENSOR ERROR faulty. MANUAL FOR See the customer’s Special
HELP Procedures and Troubleshooting
manual.
NEEDLE HOME Needle home sensor may be REFER TO Call your Beckman Coulter
SENSOR ERROR faulty. MANUAL FOR Representative.
HELP
NO PARAMETER Enter Calibration Factors or CANNOT ENTER From the F5-Optns/Select Parameters
SELECTED Enter Secondary Cal Factors CAL FACTORS screen, select the parameters to be
requested before parameters for calibrated.
calibration were selected.
NO PARAMETER Transmission of calibration CANNOT From the F5-Optns/Select Parameters
SELECTED factors requested, but TRANSMIT screen, select the parameters to be
parameters for calibration were calibrated and transmitted.
not selected.
PAK OUT Out-of-PAK signal detected from REPLACE PAK, 1. Replace the PAK.
reagent sensor; insufficient PAK UPDATE FILE, 2. Update the reagent file.
reagent to perform another PRIME
3. Prime the PAK.
cycle.
Service only: If the PAK is not empty,
check for -
pick-up tube.
supply line.
7.1-20 PN 4235961E
TROUBLESHOOTING
ERROR MESSAGES 7
Table 7.1-6 System Error Messages - J through P (Continued)

PARTIAL Two attempts to aspirate from CHECK THE 1. Ensure the specimen tube has
ASPIRATION single tube unsuccessful. SPECIMEN AND sufficient whole blood.
SYSTEM 2. Delete one or more samples
3. Rerun the specimen in the Primary
mode.
4. If error recurs, rerun that specimen
in the Secondary mode.
Service only: If this message occurs for
most or all samples:
1. Cycle a sample in the Primary
mode, and observe the Diluter
during aspiration for normal
operation.
2. If the Diluter is aspirating correctly,
check the blood detectors as
instructed under Heading 4.21,
BLOOD/BUBBLE DETECTORS GAIN
ADJUSTMENT.
PATIENT ID Attempt made to aspirate ENTER ID? YES, Enter patient ID and resume processing.
REQUIRED FOR sample without entering patient CANCEL
PROCESSING ID.
PREVIOUS SAMPLE Previous sample’s result not CHECK HOST 1. If this error occurred and you do
NOT XMITTED TO transmitted to host computer. CABLES/SETTING not have a host computer
HOST connection, reset the system.
2. Verify the host computer cables are
plugged firmly into the correct
jacks. See Installation Chapter of
the Reference manual.
3. If error recurs, verify host
computer setting on Host
Computer Definition window.
PRN TX BUFF Memory buffer to send data to CHECK PRINTER Verify the printer cables are plugged
REQUEST NOT Ticket Printer requested but not CABLES firmly into the correct jacks. See
SUCCESSFUL obtained. Installation Chapter of the Reference
manual.
PN 4235961E 7.1-21
TROUBLESHOOTING
ERROR MESSAGES
Table 7.1-7 System Error Messages - R

RAW DATA SPACE Space where raw data stored is BACKUP AND Call your Beckman Coulter
FULL-CAPTURE full; raw data capture canceled. DELETE RAW Representative.
OFF DATA Service only:
1. Backup existing RAW.DAT file to
Bernoulli diskette.
2. From the Raw Data menu, delete
raw data files from hard disk.
RAW DATA SWITCH Raw data sent; however, raw TURN RAW Call your Beckman Coulter
OFF data switch option turned OFF at SWITCH ON Representative.
DMS. Service only: On the Raw Data Option
screen, turn raw data switch option ON.
RAW DATA The system error detected RESET THE Reset the system.
TRANSMISSION during raw data transmission. SYSTEM
ERROR The system locked up.
RAW FILE TOO RAW.DAT file too large. The RESET THE Reset the system.
LARGE system locked up. SYSTEM
RBC AND WBC BATH RBC and WBC baths overflowed. CHECK ERROR See the customer’s Special Procedures
OVERFLOW LOG AND and Troubleshooting manual.
FLUIDICS
RBC BATH RBC bath overflowed. CHECK ERROR See the customer’s Special Procedures
FLUIDICS
RBC VALUE MUST Out-of-range RBC value entered. PLEASE REENTER Enter valid RBC value.
BE > 0 AND < OR RBC VALUE
EQUAL TO 9.99
RBC VALUE MUST Out-of-range RBC value entered. PLEASE REENTER Enter valid RBC value.
BE > 0 AND < OR RBC VALUE
EQUAL TO 999
RED A/I/V OUT OF Red aperture current voltage (A/ PERFORM Perform System Test. See the
RANGE I/V) out of established operating SYSTEM TEST customer’s Special Procedures and
range. Troubleshooting manual. If the problem
Range is 141.5 to 169.1 V. recurs, call your Beckman Coulter
Representative.
REPRODUCIBILITY Attempt made to change TO CHANGE Change mode of operation by pressing
IS ACTIVE Reproducibility mode of MODE, PRESS Ñ Stop.
operation without data in F9-STOP
reproducibility file and another
Reproducibility mode active.
REPRODUCIBILITY Reproducibility cycle completed CLEAR TABLE 1. Clear the table (delete 60 sample
TABLE FULL 60 samples; attempt made to CLEAR TABLE/ table).
run another sample. RERUN TEST 2. Reselect the appropriate mode of
operation to rerun the desired test.
7.1-22 PN 4235961E
TROUBLESHOOTING
ERROR MESSAGES 7
Table 7.1-7 System Error Messages - R (Continued)

RETIC VOLTAGE Retic voltage out of established PERFORM Perform System Test. See the
ERROR [XX.XX] operating range. SYSTEM TEST customer’s Special Procedures and
Note: XX.XX = Range is 0.20 to 1.20 Vdc. Troubleshooting manual.
actual reading.
RETRIES EXCEEDED Three attempts to download RESET THE Reset the system.
IN DILUTER diluter table from DMS to SYSTEM
DWNLD instrument failed. The system
locked up.
RETRIES FAILED Three attempts to download 196 RESET THE Ensure the lower front door is closed.
196CODE DWNLD code from DMS to instrument SYSTEM Reset the system.
TO 376 failed. The system locked up.
RF VOLTAGE LOW RF voltage dropped below lower PERFORM 1. Check that correct diluent is in use.
limit due to weak RF oscillator SYSTEM TEST Blood bank saline can cause this
circuit. error.
2. Check that the diluent is primed.
3. Perform System Test to confirm the
problem. See the customer’s
4. A clogged flow cell can cause this
error. Clear the flow-cell clog. See
the customer’s Special Procedures
and Troubleshooting manual.
5. If the RF voltage is still low, call
your Beckman Coulter
Representative.
RINSE BLOCK Rinse block did not return to CONTACT Check if the manual aspirate probe is
ERROR home position. SERVICE fully extended. Check if the aspirate
probe is bent. Call your Beckman Coulter
Representative.
ROCKER BED NOT Cassette placed directly onto RESTART THE Reinitiate the "Clear the Bed/Autoloader
EMPTY rocker bed after a “Clear the FUNCTION Home" function to automatically clear
Note: Applicable to Bed/Autoloader Home” function the bed.
MAXM analyzer initiated.
with Autoloader
module only.
PN 4235961E 7.1-23
TROUBLESHOOTING
ERROR MESSAGES
Table 7.1-8 System Error Messages - S

SAMPLE HANDLER Error detected by sample CLEAN BARCODE 1. Clean the bar-code sensors. See
BARCODE SENSOR handler bar-code sensor. Bar SENSORS the customer’s Special Procedures
ERROR code detection sensors should and Troubleshooting manual.
Note: Applicable to be cleaned or are faulty. 2. To resume operation, access the
MAXM analyzer appropriate screen, reselect the
with Rotary mode of operation, and rerun the
Cap-Pierce module samples.
only. Note: The system may be used with
faulty sensor; however, all bar-coded
information must be entered manually.
SAMPLE HANDLER Instrument received illegal RESET THE Reset the system.
COMM. FAILURE message or has not received any SYSTEM
message from sample handler.
SAMPLE HANDLER Analyzer lost communication RESET THE Reset the system.
COMMUNICATION with sample handler. The system SYSTEM
ERROR locked up.
SAMPLE HANDLER Error detected by sample CLEAN OBJECT 1. Clean object-detection sensors.
HAND DETECT handler hand detect sensor. DETECTION See the customer’s Special
ERROR Object-detection sensors should SENSORS Procedures and Troubleshooting
Note: Applicable to be cleaned or are faulty. manual.
only. samples.
SAMPLE HANDLER Instrument detected severe RESET THE Reset the system.
NOT OPERATIONAL sample-handler error. SYSTEM
SAMPLE HANDLER Error detected by CHECK NEEDLE Call your Beckman Coulter
SENSOR 16 ERROR sample-handler sensor 16. ALIGNMENT Representative.
Needle not aligned.
SAMPLE HANDLER Error detected by sample- CLEAN BED 1. Clean the Autoloader module
SENSOR 17 ERROR handler sensor 17. Bed position POSITION bed-position sensors. See the
Note: Applicable to sensor should be cleaned or is SENSOR customer’s Special Procedures and
MAXM analyzer faulty. Troubleshooting manual.
with Autoloader 2. To resume operation, access the
module only. appropriate screen, reselect mode
of operation, and rerun the sample.
SAMPLE HANDLER Instrument did not respond to RESET THE Ensure the lower front door is closed.
TIMEOUT ERROR sample handler within specified SYSTEM Reset the system.
time.
7.1-24 PN 4235961E
TROUBLESHOOTING
ERROR MESSAGES 7
Table 7.1-8 System Error Messages - S (Continued)

SAMPLE NOT Sample’s result not transmitted CHECK HOST Verify the host computer cables are
TRANSMITTED TO to host computer. CABLES plugged firmly into the correct jacks. See
HOST Installation Chapter of the Reference
manual.
SEC CBC Secondary CBC calibration CLEAR TABLE 1. Clear the table (delete 60 sample
CALIBRATION completed 60 samples; attempt CLEAR TABLE/ table).
TABLE FULL made to run another sample. RERUN TEST 2. Reselect the appropriate mode of
SEND TO Transmission of 196 code from RESET THE Reset the system.
INSTRUMENT DMS to instrument failed. The SYSTEM
FAILED - 196CODE system locked up.
SERVICE Service Reproducibility cycle CLEAR TABLE 1. Clear the table (delete 60 sample
REPRODUCIBILITY completed 60 samples; attempt CLEAR TABLE/ table).
TABLE FULL made to run another sample. RERUN TEST 2. Reselect the appropriate mode of
SHEATH PRESSURE Sheath pressure out of CHECK/ADJUST 1. Perform System Test to check the
OUT OF RANGE established operating range. SHEATH sheath pressure. See the
Range is 5.80 to 6.20 psi. PRESSURE customer’s Special Procedures and
2. Adjust the sheath pressure. See the
SHEATH TANK Sheath tank is empty. CHECK/PRIME 1. Check the sheath tank and tubing
EMPTY DILUENT for any obvious problems.
2. Prime the diluent. See the
SHUTDOWN Attempt made to select sample MUST PERFORM 1. Run Startup. See Operator's Guide.
PERFORMED mode through F3-Run window STARTUP 2. Select the desired mode to run
PREVIOUSLY before Startup cycle performed. tests.
(Shutdown cycle performed
previously and DMS software
disabled sample cycles. Startup
cycle must be performed.)
SOFTWARE When Startup initiated, PERFORM Run the Autoloader Test function. See
AUTOLOADER Autoloader module failed at least AUTOLOADER the customer’s Special Procedures and
CHECK FAILED one of checks performed. TEST ROUTINE Troubleshooting manual.
Note: Applicable to
MAXM analyzer
with Autoloader
module only.
PN 4235961E 7.1-25
TROUBLESHOOTING
ERROR MESSAGES
Table 7.1-8 System Error Messages - S (Continued)

STOP SWITCH Stop switch activated when RESELECT Access the appropriate screen and
ACTIVATED Autoloader module not in Stop FUNCTION reselect desired function. Ensure the
Note: Applicable to mode. switch cable is not disconnected.
MAXM analyzer
with Autoloader
module only.
SYSTEM Instrument did not respond to CHECK Call your Beckman Coulter
BACKGROUND background request in specified DIGIBOARD Representative.
TIME OUT time. Service only: Check the Digiboard cables
for proper connection.
7.1-26 PN 4235961E
TROUBLESHOOTING
ERROR MESSAGES 7
Table 7.1-9 System Error Messages - T

TEMP: The system temperature error CYCLE NEXT Run next sample cycle.
AMBIENT=XX.XX occurred. SPECIMEN
LYSE=XX.XX
Note: XX.XX =
actual reading.
TEMP: Peltier module error occurred. PERFORM CBC Run CBC sample with DIFF OFF.
AMBIENT=XX.XX XX.XX = actual ambient and lyse ONLY
LYSE=XX.XX readings.
Note: XX.XX =
actual reading.
TEST MODE The system put into Stop mode RESTARTING Restart the test. Aspiration count begins
INTERRUPTED before requested number of TEST WILL ASP # at selected number.
aspirations per tube completed. SELECTED
TICKET PRINTER Ticket Printer did not accept CHECK TICKET Verify the Ticket Printer is plugged in,
NOT READY print request. PRINTER properly connected, and has a ticket in
the slot.
TKT.CFG FILE I/O An input/output error occurred RESET THE Reset the system.
ERROR on TKT.CFG file. The system SYSTEM
locked up.
TRANSMIT FAILED - Transmission of 196 code from RESET THE Reset the system.
196 CODE DMS to instrument failed. The SYSTEM
system locked up.
TRANSMIT FAILED - Transmission of 376 code from RESET THE Reset the system.
376 CODE DMS to instrument failed. The SYSTEM
system locked up.
TRANSMIT FAILED - Transmission of diluter table RESET THE Reset the system.
DILUTER TABLE from DMS to instrument failed. SYSTEM
The system locked up.
TRANSMIT PORT Transmit port not available for CHECK SYSTEM Verify the cables are plugged firmly into
NOT AVAILABLE transmission between DMS and CABLES the correct jacks. See Installation
instrument. Chapter of the Reference manual.
TRANSMIT TO 376 Transmission of 196 code from RESET THE Reset the system.
FAILED - 196 CODE DMS to instrument failed. The SYSTEM
system locked up.
TUBE AVAILABLE Tube-available sensor may be REFER TO 1. Clean the tube available sensor. See
SENSOR ERROR faulty. MANUAL FOR the customer’s Special Procedures
Note: Applicable to HELP and Troubleshooting manual.
MAXM analyzer 2. If error recurs, call your Beckman
with Rotary Coulter Representative.
Cap-Pierce module
only.
PN 4235961E 7.1-27
TROUBLESHOOTING
ERROR MESSAGES
Table 7.1-10 System Error Messages - U

UNABLE TO OPEN/ DMS download code not able to RESET THE Reset the system.
READ open/read 196CODE.HEX file SYSTEM
196CODE.HEX and retrieve 196 code revision
number. The system locked up.
READ open/read 376CODE.HEX file SYSTEM
376CODE.HEX and retrieve 376 code revision
READ DILUTER open/read DILUTE.TBL file and SYSTEM
TBL retrieve diluter table revision
UNABLE TO DMS could not process RETRY THE 1. Press Ñ Stop to verify the mode
PROCESS requested function because a FUNCTION was not previously selected.
REQUEST MODE-NOT-ACCEPTED or 2. Reselect the desired function.
START-NOT-ACCEPTED signal
received from instrument.
UNIDENTIFIED Internal and unidentifiable RESET THE Reset the system.
ERROR [XXXX] instrument error with no SYSTEM
Note: IXXX = message detected by CPU. The
instrument internal system locked up.
code; SXXX =
sample handler
internal code.
UNIDENTIFIED S44 Failure in tube-available switch. Internal code only. Call your Beckman Coulter
ERROR Probably due to: representative.
Note: Applicable to r Poor contacts. Service only: Inspect the switch and
MAXM analyzer r A very narrow tolerance of repair if necessary.
with Autoloader switch contact within
module only. "walks" along rocker bed.
r Slippage of cassette during
indexing.
Possibly due to switch failure.
UNIDENTIFIED S45 Failure in tube-available switch Internal code only. Call your Beckman Coulter
ERROR after indexing and detection of representative.
Note: Applicable to last tube in cassette. Probably Service only: Inspect the switch and
MAXM analyzer due to: repair if necessary.
with Autoloader r Poor contacts.
module only. r A very narrow tolerance of
switch contact within
"walks" along rocker bed.
r Slippage of cassette during
indexing.
7.1-28 PN 4235961E
TROUBLESHOOTING
ERROR MESSAGES 7
Table 7.1-10 System Error Messages - U (Continued)

UNIDENTIFIED S55 Over-allocation of all available Internal code only. If error occurs frequently, determine
ERROR software timers. frequency of error and call your Beckman
UNIDENTIFIED S57 False interrupt associated with Internal code only. Call your Beckman Coulter
ERROR either door or stop switches on Representative.
Sample Handler. Service only: Ensure integrity of door
r Could be due to noise and stop connections.
associated with twisted
pair wires or connector on
cable attached to stop
switch.
r Less likely due to any
electrical noise induced
into C196
microprocessor’s external
interrupt pin.
UNLOAD ELEVATOR Unload (left) elevator not CHECK ELEVATOR Verify performance of the elevator
FAILURE functioning. Unload elevator’s MECHANISM mechanism. See the customer’s Special
Note: Applicable to motor may have failed. Procedures and Troubleshooting manual.
MAXM analyzer
with Autoloader
module only.
UNLOAD STACK Unloading bay reached EMPTY THE Remove the cassettes from the
FULL maximum capacity. UNLOAD STACK unloading bay so additional cassettes
Note: Applicable to can be dispensed from the rocker bed.
MAXM analyzer
with Autoloader
module only.
PN 4235961E 7.1-29
TROUBLESHOOTING
ERROR MESSAGES
Table 7.1-11 System Error Messages - V through Z

WASTE CONTAINER Waste container is full. EMPTY WASTE
FULL CONTAINER WARNING Biohazardous contamination
could occur from contact with the old
waste container and its associated
tubing if not handled with care. Wear
protective gear. Avoid skin contact.
Clean up spills immediately. Dispose of
the contents of the waste container
according to your local regulations and
acceptable laboratory procedures.
Empty the waste container, then resume

operation. Check that the harness is
connected to the waste pickup tube. See
the customer’s Special Procedures and
WATER TRAP DID Water trap bleed solenoid CONTACT Call your Beckman Coulter
NOT BLEED activated, but pressure did not SERVICE Representative.
[XX.XX] drop in specified time.
Note: XX.XX =
actual reading.
WBC BATH WBC bath overflowed. CHECK ERROR See the customer’s Special Procedures
FLUIDICS
WHITE A/I/V OUT OF White aperture current voltage PERFORM 1. Perform System Test to confirm the
RANGE (A/I/V) not within established SYSTEM TEST problem. See the customer’s
operating range. Range is 100.6 Special Procedures and
to 129.6 V. Troubleshooting manual.
2. If the white A/I/V is still out of
range, call your Beckman Coulter
Representative.
WORKLIST ERRORS Three consecutive NO READ or CLEAR ERRORS/ 1. Check the labels for proper
NOT CLEARED NO MATCH errors, or 10 total CHECK LABELS/ positioning, and check the worklist
NO READ, NO MATCH or PART RESTART for NO MATCH errors.
ASP errors occurred and are CLEAR ERRORS/ 2. Delete one or more samples
stored in Worklist file. CHECK WLIST/ associated with the errors from the
RESTART worklist.
WORKLIST FULL Maximum number of RUN SAMPLES Remove some samples with errors, or
preassigned samples (300) and clear the worklist by running samples.
worklist errors (10) exceeded.
WRONG ASSAY Control assay sheet used is not CHECK FOR Verify the appropriate assay sheet is
SHEET IN USE for MAXM analyzer. CORRECT ASSAY being used.
SHEET
7.1-30 PN 4235961E
TROUBLESHOOTING
ERROR MESSAGES 7
Table 7.1-11 System Error Messages - V through Z (Continued)

WRONG DIGIBOARD Digiboard incorrectly installed or CHECK Call your Beckman Coulter
SOFTWARE faulty. DIGIBOARD Representative.
WRPORT UNAVAIL Analyzer not communicating RESET THE Reset the system.
FOR 376 CODE with Digiboard. The system SYSTEM
locked up.
PN 4235961E 7.1-31
TROUBLESHOOTING
ERROR MESSAGES
Table 7.1-12 Internal Instrument Codes
Code Description Code Description

70 Strange response/command from monitor. 92 Analysis start error (no memory).
71 Strange response/command from program control. 93 Analysis end error (undefined).
72 Strange response/command from analysis. 95 Lyse temperature error (warning).
73 Strange response/command from VCS acquisition. 96 Compressor error.
74 Strange response/command from CBC acquisition. 98 Too much pressure lost in acquisition.
75 Bad response/command from DMS. 99 High vacuum below 10 in. Hg.
76 DMS timeout error. 100 FC flow cell clog.
78 Specified control program does not exist. 101 PC1 flow cell clog.
79 Bad parameter for mode or table. 102 PC2 flow cell clog.
80 Illegal instruction in control program. 103 RBC voteout one sample.
81 Errors in execution control program. 104 WBC voteout one sample.
82 Bad sample handler super program error. 105 Platelet voteout one sample.
84 Tube bar-code error. 106 MPV voteout one sample.
85 Request bar code verification. 107 FD flow cell clog.
86 Bad sample handler command (prompt). 108 PC3 flow cell clog.
87 Bad sample handler response. 110 Sample handler error aborted.
88 Bad sample handler sensor/mechanism. 111 Sample handler prompt aborted.
90 CBC start error (no memory). 112 Unloading bay is full.
91 VCS start error (no memory).
7.1-32 PN 4235961E
TROUBLESHOOTING
FLOW-CELL ERRORS FC, PC1, AND PC2 7
7.2 FLOW-CELL ERRORS FC, PC1, AND PC2
The three flow-cell errors, FC (Full Clog), PC1 (Partial Clog 1), and PC2 (Partial Clog 2), are
displayed on the DMS Run Sample screen, immediately below the WBC scatterplot. For all
three errors, the diff parameter results (both % and #) are replaced with the (:::::) code.
PC1 and PC2 are based on the DC count and time. FC is triggered by a special circuit, the
Clog Detector, on the RF/DC Detector Preamp. See Table 7.2-1 for the specific conditions that
trigger a flow-cell error and a list of typical causes.
Table 7.2-1 Flow-Cell Errors
Error Condition Typical Causes

FC Overvoltage is detected in the RF/DC current in the flow 1. Particulate matter plugging flow cell.
cell. (The current is not flowing correctly between the 2. Interruption in RF/DC aperture current from:
two electrodes in the flow cell.)
r Bubbles.
Note: When an FC error occurs, the RF/DC current is r Open circuit.
turned off. r Coaxial cable problems.
When an FC is detected, the instrument stops the cycle 3. Defective RF tube inside the RF/DC Detector
and starts an autoclear. if the FC error is not cleared Preamp card.
after three attempts, the instrument stops.
4. Incorrectly adjusted Clog Detector circuit. To adjust
the circuit, refer to Clog Detector Circuit Adjustment
under Heading 4.11, RF DETECTOR PREAMP CARD
ADJUSTMENTS.
5. Failure of the Clog Detector circuit.
PC1 The following conditions must exist to generate a 1. Obstructed sample line to flow cell.
PC1 error: 2. Partially plugged flow cell.
r The WBC is >1K and <56K. 3. Sample pressure too low (flow not fast enough).
r The DC count time exceeds the expected count time 4. Sheath pressure too high (sample flow restricted).
by greater than 50% where -
[(7000/WBC) x 10 = Expected Time].
For example, if the count time for a WBC of 7,000 is
>15 s (which is 50% more than the expected
10 seconds), a PC1 occurs.
When PC1 is detected three times consecutively, the
instrument initiates an autoclear routine at the end of
the third cycle. However, due to the overlap of
instrument cycles, the autoclear routine does not
actually occur until the end of the fourth cycle.
PN 4235961E 7.2-1
TROUBLESHOOTING
FLOW-CELL ERRORS FC, PC1, AND PC2
Table 7.2-1 Flow-Cell Errors (Continued)
Error Condition Typical Causes

PC2 The following conditions must exist to generate a 1. Obstructions in the lower sheath input (sample
PC2 error: rushes through the flow cell).
r The WBC is >1K and <56K. 2. Chemistry imbalance caused by:
r Either 8192 cells accumulate within <1.0 second or r Incorrect Erythrolyse II reagent, StabiLyse
zero (0) cells accumulate within 1.0 second. reagent, and/or blood-sample volumes.
When PC2 is detected, the instrument initiates an r Incorrect Erythrolyse II reagent, StabiLyse
autoclear routine at the end of the cycle. However, due reagent, and/or blood sample delivery.
to the overlap of instrument cycles, the autoclear r Incorrect mixing in the mixing chamber.
routine does not actually occur until the end of the r Incorrect rinsing and/or draining at mixing
next cycle. chamber.
r Interferences in the blood specimen, such as:
t Lipemia.
t Extreme chemistry imbalances.
t Drugs.
r Condition of reagents, such as:
t Diluent frozen and improperly mixed
after thawing.
t Reagents contaminated.
t Reagents outdated.
7.2-2 PN 4235961E
TROUBLESHOORTING
DC, RF, OR LS NOISE PROBLEMS 7
7.3 DC, RF, OR LS NOISE PROBLEMS
Purpose
Use this procedure to locate the problem when the RMS noise checks for RF, DC, or LS are out
of specifications.
B Service Disk
B 20 MHz dual-trace oscilloscope
B True RMS meter
DC Noise Troubleshooting Checks

2. Connect Channel 1 of the oscilloscope to the channel on the VCS PROCESSOR card.
3. Set the V/div to the 20-mV scale.
4. Set the scope’s trigger source to Ch 1.
5. While the system is idle, step through the time base settings from 5 millisecond/div
through 20 microseconds/div. At each setting, vary the trigger level and look for periodic
waveforms. See Figures 7.3-1 and 7.3-2 for examples of acceptable and unacceptable
static DC noise. Repeat if necessary.
Figure 7.3-1 Acceptable DC Noise Display Figure 7.3-2 Unacceptable DC Noise Display
6. If you see any periodic waveforms, find and eliminate the cause.
7. Set the scope’s trigger source to LINE and repeat steps 5 and 6.
8. Set the scope’s trigger source back to Ch 1.
9. Cycle a sample of clean diluent in the Diff-Latex Calibration mode.
10. While the system is counting, step through the time base settings from
5 milliseconds/div through 20 microseconds/div. At each setting, vary the trigger level
and look for periodic waveforms. See Figures 7.3-1 and 7.3-2 for examples of acceptable
and unacceptable dynamic DC noise. Repeat if necessary.
PN 4235961E 7.3-1
TROUBLESHOORTING
DC, RF, OR LS NOISE PROBLEMS
12. Set the scope’s trigger source to LINE and repeat steps 9 through 11.
13. If you corrected a noise problem, do the Diff and Retic RMS Noise Checks under
Heading 4.12, RMS NOISE CHECKS, to verify the DC, RF and LS noise levels are within
specifications.
14. If you did not correct the noise problem, continue troubleshooting. Go to RF Noise
Troubleshooting Checks below.
RF Noise Troubleshooting Checks

1. Connect Channel 1 of the oscilloscope to the channel on the VCS PROCESSOR card.
2. Set the V/div to the 50-mV scale.
3. Set the trigger source to Ch 1.
4. While the system is idle, step through the time base settings from 5 milliseconds/div
static RF noise. Repeat if necessary.
6. Set the scope trigger source to LINE and repeat steps 4 and 5.
7. Set the trigger source back to Ch 1.
Figure 7.3-3 Acceptable Static RF Noise Display Figure 7.3-4 Unacceptable Static RF Noise Display
8. Cycle a sample of clean diluent in the Diff-Latex Calibration mode.

9. While the system is cycling, step through the time base settings from 5 milliseconds/div
waveforms. See Figure 7.3-5 for an example of acceptable dynamic RF noise. Repeat if
necessary.
7.3-2 PN 4235961E
TROUBLESHOORTING
Figure 7.3-5 Acceptable Dynamic RF Noise
10. If you see any periodic waveforms, or if the noise level is above specifications:
a. Ensure C1 is adjusted correctly. Refer to C1 Adjustment under Heading 4.11, RF
DETECTOR PREAMP CARD ADJUSTMENTS.
b. Using Table 7.3-1 and Figures 7.3-6 through 7.3-16 as troubleshooting aids, find
and eliminate the cause of the noise.
11. If you corrected a noise problem, do the Diff and Retic RMS Noise Checks under
specifications.
12. If you did not correct the noise problem, continue troubleshooting. Go to LS Noise
Troubleshooting Checks at the end of this section.
Table 7.3-1 RF Troubleshooting Table
Sample Type* Problem Cause Action See Figures...

Diluent 60 Hz Loose grounds Check grounding 7.3-6 and 7.3-7
LATRON Random baseline Fluidics leaks or Fix leaks or perform 7.3-8 and 7.3-9
Control bounce bubbles purge
Diluent RMS noise (mV) RF Preamp noise Check C1 Adjust 7.3-10 and 7.3-11
38 <RF <100 Replace:
1. Vacuum tube
2. RF Preamp
Diluent RMS noise (mV) RF Preamp noise Replace: 7.3-10 through 7.3-14
RF >100 random or 1. Vacuum tube
continuous
2. RF Preamp
Diluent RMS noise (mV) Shunts or leaky flow cell Clean QD7 7.3-15 and 7.3-16
RF >100 random Perform: Purge
* Obtain all samples in the Diff-Latex Calibration mode.
PN 4235961E 7.3-3
TROUBLESHOORTING
Figure 7.3-6 RF 60 Hz Figure 7.3-6 is an example of a normal LATRON

control run. The RMS noise is 22.0 mV. Notice the
RF base.
Figure 7.3-7 RF 60 Hz with Noise Spikes In Figure 7.3-7, notice with the 60 cycles the large
spikes and how the baseline is distorted. The RMS
noise increased to 63 mV.
Figure 7.3-8 Normal RF Random Baseline Bounce Figure 7.3-8 is an example of a normal LATRON
control run. The RMS noise is 22.0 mV. Notice the
baseline after the RF pulse.
7.3-4 PN 4235961E
TROUBLESHOORTING
Figure 7.3-9 RF Output with bubble Trapped in Sheath 2 Figure 7.3-9 shows the RF output while running
LATRON control with a bubble trapped in
sheath 2. The RMS noise increased to 24.5 mV.
Notice the bounce in the baseline after the
RF pulse.
Note: The single pulse with the doubled amplitude

is caused by two LATRON control particles
simultaneously passing through the flow cell.
Figure 7.3-10 Normal RF Baseline While Cycling Diluent Figure 7.3-10 shows a normal RF baseline while
cycling diluent. The RMS noise is 22.0 mV.
Figure 7.3-11 Noisy RF Baseline While Cycling Diluent Figure 7.3-11 shows a noisy RF baseline while
cycling diluent; the RMS noise is now 45 mV.
Notice the amplitude increased in the baseline. In
this case the RMS noise is steady.
PN 4235961E 7.3-5
TROUBLESHOORTING
Figure 7.3-12 Noisy RF Baseline While Cycling Diluent Figure 7.3-12 shows a noisy RF baseline while
(RF Noise >100 mV) cycling diluent; the RMS noise is now >100 mV
and erratic. Notice the amplitude increased in the
baseline. In this case the RMS noise is unsteady as
is the baseline.
Figure 7.3-13 LATRON Control Sample with a Good RF Figure 7.3-13 is a LATRON control sample from a
Tube and Box system with a good RF tube and box. Notice the
mean-to-mode channel difference, the low CV and
the narrow histogram.
Figure 7.3-14 LATRON Control Sample with a Bad RF In the LATRON control sample shown in
Tube and Box Figure 7.3-14 the RF tube is bad, but the box can
cause the same problem.
The mean-to-mode difference increased, as well as

the CV. Also the histogram now has a foot to the
right. In addition, the OP histogram is wider and
not as smooth.
7.3-6 PN 4235961E
TROUBLESHOORTING
Figure 7.3-15 RF Output Running LATRON Control (Leak Figure 7.3-15 shows the RF output while running
at QD7) LATRON control with a leak at QD7. The RMS
noise increased to 23.5 mV. Notice the bounce in
the baseline after the RF pulse.
Figure 7.3-16 LATRON Control Run with DC Gain Drop In the LATRON control run shown in
Figure 7.3-16, observe the DC gain drop. The CV
for both DC and RF increased. And, on all channels
there is noise to the right of the LATRON control
populations. The OP histogram too is wider,
shifted to the right and not as smooth.
LS Noise Troubleshooting Checks
WARNING The laser beam can cause eye damage if viewed either directly or indirectly from reflective
surfaces (such as a mirror or shiny metal surface). Ensure the laser cover is on and the front doors of the
Main Unit are closed while you do the LS Noise Troubleshooting Checks.
1. Ensure the laser cover is on and the front doors of the Main Unit are closed.
3. While the system is counting, measure the LS offset voltage with a DMM and ensure that
the voltages do not exceed the limits specified for Diff mode in Table A.1-19, LS Offset
Voltage/Laser On Current Checks.
PN 4235961E 7.3-7
TROUBLESHOORTING
4. If the LS offset voltage exceeds the limits:

a. Clean the flow cell as directed under Heading 4.13, FLOW-CELL CLEANING.
b. If you still cannot achieve the specifications, realign the laser/flow cell as directed
under Heading 4.15, LASER/FLOW CELL ALIGNMENT.
5. If the MAXM analyzer is a Retic analyzer, cycle a sample of diluent in the Retic-Latex
Calibration mode and repeat step 3.
6. Set up the oscilloscope:
a. Connect Channel 1 of the oscilloscope to the LS channel (S) on the VCS
PROCESSOR card.
b. Set the V/div to the 20-mV scale
c. Set the trigger source to Ch 1.
7. Ensure that the Diff mode is enabled.
8. While the system is idle, step through the time base settings from 5 milliseconds/div
static LS noise. Repeat if necessary.
Figure 7.3-17 Acceptable Static LS Noise Display Figure 7.3-18 Unacceptable Static LS Noise Display
Note: When the time base is set at 20 microseconds/div, a 25-kHz waveform may be
observed on some instruments. This can be caused by the Laser Power Supply.
10. Set the scope’s trigger source to LINE. Repeat steps 8 and 9.
11. Set the scope’s trigger source back to Ch 1.
13. While the system is counting, step through the time base settings from
5 milliseconds/div through 20 microseconds/div. At each setting, vary the trigger level
and look for periodic waveforms. See Figure 7.3-19 for an example of acceptable
dynamic LS noise.
7.3-8 PN 4235961E
TROUBLESHOORTING
Figure 7.3-19 Acceptable Dynamic LS Noise Display
Note: When the time base is set at 20 microseconds/div, a 25-kHz waveform may be
observed on some instruments. This can be caused by the Laser Power Supply.
15. Set the scope’s trigger source to LINE. Repeat steps 12 through 14.
16. After correcting the noise problem, do the Diff and Retic RMS Noise Checks under
specifications.
PN 4235961E 7.3-9
TROUBLESHOORTING
7.3-10 PN 4235961E
TROUBLESHOOTING
DC, RF, OR LS LATEX CALIBRATION PROBLEMS 7
7.4 DC, RF, OR LS LATEX CALIBRATION PROBLEMS
Use Table 7.4-1 to locate the problem when you cannot obtain acceptable DC, RF, or LS
results during diff and retic latex calibration. When you have corrected the problem, repeat
the DC, RF, and LS gain adjustments as directed in Diff and Retic Latex Calibration and
Table 7.4-1 RF, DC, or LS Latex Calibration Troubleshooting Table
Problem Probable Cause Action

DC CVs are outside the 1. Plugged sheath restrictor 1. Replace the sheath 2 restrictor tubing.
specifications. tubing. 2. Adjust the sheath pressure regulator and cycle LATRON
2. Flow cell (sheath and control until you obtain the minimum CVs for DC.
sample) pressure settings 3. Readjust the sample pressure as directed for Sample
are not optimal. Pressure Adjustment under Heading 4.10, VCS
FLOW-RATE ADJUSTMENT.
DC and/or RF Plugged sheath restrictor coil. Check the sheath 2 restrictor coil for a plug.
mean-to-modes are
outside the
specifications.
LS CVs or mean-to-modes 1. Noise on the LS channel. 1. Measure the RMS noise as directed in Diff and Retic
are outside the 2. Dirty flow cell. RMS Noise Checks under Heading 4.12, RMS NOISE
specifications. CHECKS.
3. Misaligned laser/flow cell.
2. Check the laser on current/LS offset voltage as directed
in Laser On Current/LS Offset Voltage Check under
Heading 4.23, VOLTAGE CHECKS AND ADJUSTMENTS.
3. If the problem persists, clean the flow cell as directed
under Heading 4.13, FLOW-CELL CLEANING.
4. If the problem still persists, align the flow cell as
directed under Heading 4.15, LASER/FLOW CELL
ALIGNMENT.
RF CVs are outside the Noise on the RF channel. 1. Measure the RMS noise as directed in Diff and Retic
specifications. RMS Noise Checks under Heading 4.12, RMS NOISE
CHECKS.
2. Replace the vacuum tube in the RF Detector Preamp
card.
3. Replace the RF Detector Preamp card.
PN 4235961E 7.4-1
TROUBLESHOOTING
DC, RF, OR LS LATEX CALIBRATION PROBLEMS
7.4-2 PN 4235961E
TROUBLESHOOTING
REPORTED INSTRUMENT PROBLEMS AND SOLUTIONS 7
7.5 REPORTED INSTRUMENT PROBLEMS AND SOLUTIONS
The suggestions in Table 7.5-1, Troubleshooting Reported Instrument Problems, are meant as
a guideline only, and are not necessarily the solution for the same reported symptoms.
Table 7.5-1 Troubleshooting Reported Instrument Problems
Problem Symptom Corrective Action

Bar-code reader not The bar-code reader does not 1. Power down and power up the instrument as directed in
reading cassette turn on. All other functions are Power Down/Power Up the System under Heading 4.1,
and tube bar-code working correctly. GUIDELINES FOR SERVICING THE MAXM ANALYZER, to
labels. Note: This problem usually reset the Bar-Code Reader Decoder card.
occurs after a power 2. Reconfigure the Bar-Code Reader Decoder card as directed
interruption. under Heading 4.38, BAR-CODE READER DECODER CARD
CONFIGURATION, if necessary.
Download stops after The instrument downloads the 1. Ensure the Secondary-mode, whole-blood switch is not
376 code 376 code and stops three times stuck.
in succession. Then the 2. Repair or replace the whole-blood switch, S2.
instrument generates a
DOWNLOAD NOT
SUCCESSFUL message and an
INSTRUMENT REPLY
TIMEOUT message.
Lockups Download not successful and 1. Ensure the jumpers on the R/W/P PROC card are set as
WBC, RBC or Plt report 0.00 described in Table A.2-8, R/W/P PROC Card Jumper
Settings (See Figure A.2-10).
2. Reinstall the R/W/P PROC card, ensuring it is seated
correctly.
3. If problem persists, reseat U11 and U13 on the R/W/P
PROC card.
4. Replace the R/W/P PROC card if necessary.
Lockups - excessive Various 1. Verify the following circuit cards are at the revision level
and unexplained stated or higher:
r R/W/P PROC card - L
r I/O card - K
r DILUTER INTERFACE card - J
r 376 CPU card - N
r VCS PROCESSOR card - F
r DMS Digiboard - E
2. Verify the DMS motherboard and its revision level are
approved. Refer to the DMS Configuration Listing for
STKS™, MAXM™, AND HmX Series Systems.
PN 4235961E 7.5-1
TROUBLESHOOTING
REPORTED INSTRUMENT PROBLEMS AND SOLUTIONS
Table 7.5-1 Troubleshooting Reported Instrument Problems (Continued)
Problem Symptom Corrective Action

Reagent alarms Erroneous reagent out 1. Check the P7/J7B connector at the analog backplane and
invalid messages. ensure none of the pins are pushed in and the connector is
Note: On The Disable Reagent Sensors fully seated.
instruments with screen indicates the instrument 2. Ensure the wiring harness for the reagent optical sensors is
optical reagent is only monitoring three of the not pinched between a bracket and the main frame.
sensors only. five optical reagent sensors. 3. Ensure the jumpers on the I/O card are set as described in
I/O Card, Jumper Settings, under Heading A.2, CIRCUIT
CARDS - JUMPERS AND TEST POINTS.
4. Reinstall the I/O card, ensuring it is seated correctly.
5. Replace the I/O card if necessary.
6. Replace the Pneumatic Monitor card.
Sample Analysis and The Sample Analysis and Reformat and reload the software as directed in the DMS
Control Run screens Control Run options are not Configuration Listing for STKS™, MAXM™, AND HmX Series
are unavailable listed and the DMS screen goes Systems.
blank.
System Test does not Some of the fields on the DMS 1. Test the Digiboard with the Digiboard Diagnostics kit.
work correctly screen for the System Test do 2. Replace the Digiboard if necessary.
not update.
All other functions are working
correctly.
7.5-2 PN 4235961E
CONTENTS
8
8 PARTS LISTS, 8.1-1
8.1 MASTER PARTS LIST, 8.1-1
8.2 ILLUSTRATED PARTS, 8.2-1
ILLUSTRATIONS
8.2-1 Main MAXM Analyzer Components Illustrated, 8.2-1
8.2-2 CBC Module, Front View (See Table 8.2-2), 8.2-2
8.2-3 CBC Module, Rear View (See Table 8.2-3), 8.2-4
8.2-4 Mixing Module, Front View (See Table 8.2-4), 8.2-6
8.2-5 Mixing Module, Right Side View (See Table 8.2-5), 8.2-8
8.2-6 Mixing Module, Left Side View (See Table 8.2-6), 8.2-10
8.2-7 BSV Module with BSV that Uses Air Cylinders, Front View
(See Table 8.2-7), 8.2-12
8.2-8 BSV Module with BSV that Uses an Actuator, Front View (See Table 8.2-8), 8.2-14
8.2-9 BSV Module, Rear View (See Table 8.2-9), 8.2-16
8.2-10 BSV Module, Ball and Slide Assembly (See Table 8.2-10), 8.2-18
8.2-11 Pump Module, Old Configuration (See Table 8.2-11), 8.2-20
8.2-12 Pump Module, New Configuration (See Table 8.2-11), 8.2-22
8.2-13 Rotary Cap-Pierce Module (See Table 8.2-13), 8.2-24
8.2-14 Rotary Cap-Pierce Module, Carousel Assembly (See Table 8.2-14), 8.2-26
8.2-15 Autoloader Module, Front View (See Table 8.2-15), 8.2-28
8.2-16 Autoloader, Rear View (See Table 8.2-16), 8.2-30
8.2-17 Autoloader, Rocker Bed Hardware and Sensors (See Table 8.2-17), 8.2-32
8.2-18 Needle Assembly and Sensors (See Table 8.2-18), 8.2-34
8.2-19 Triple Transducer Module (See Table 8.2-19), 8.2-36
8.2-20 Flow Cell and Laser Assembly (See Table 8.2-20), 8.2-38
8.2-21 Main Diluter Module (See Table 8.2-21), 8.2-40
8.2-22 Electronic Power Supply, Top View (See Table 8.2-22), 8.2-42
8.2-23 Pneumatic Power Supply, Left-Side View (See Table 8.2-23), 8.2-44
8.2-24 Analyzer Module, Card Cage Components (See Table 8.2-24), 8.2-46
8.2-25 Wire Harness Components (See Table 8.2-25), 8.2-48
TABLES
8.1-1 Part Categories, 8.1-1
8.1-2 Accessories, 8.1-1
8.1-3 Autoloader Module, 8.1-2
8.1-4 Bar-Code System, 8.1-4
8.1-5 BSV Module, 8.1-5
8.1-6 CBC Module, 8.1-7
8.1-7 Circuit Cards, 8.1-8
8.1-8 Covers/Doors, 8.1-9
8.1-9 Fuses, 8.1-9
8.1-10 Interconnect Cables, 8.1-10
8.1-11 Main Diluter Module, 8.1-11
8.1-12 Miscellaneous Hardware, 8.1-12
8.1-13 Mixing Module, 8.1-15
8.1-14 Options/Upgrade Kits, 8.1-16
PN 4235961E 8-i
CONTENTS
8.1-15 Pickup Tubes and Sensors, 8.1-16

8.1-16 Power Supplies, 8.1-17
8.1-17 Pump Module, 8.1-18
8.1-18 Rotary Cap-Pierce Module, 8.1-19
8.1-19 Shielded Reagent Lines, 8.1-21
8.1-20 Software, 8.1-21
8.1-21 Tools and Supplies, 8.1-22
8.1-22 Triple Transducer Module, 8.1-23
8.1-23 Tubing, 8.1-25
8.2-1 Illustrations Not Referenced from Figure 8.2-1, 8.2-1

8.2-2 CBC Module, Front View (See Figure 8.2-2), 8.2-3
8.2-3 CBC Module, Rear View (See Figure 8.2-3), 8.2-5
8.2-4 Mixing Module, Front View (See Figure 8.2-4), 8.2-7
8.2-5 Mixing Module, Right Side View (See Figure 8.2-5), 8.2-9
8.2-6 Mixing Module, Left Side View (See Figure 8.2-6), 8.2-11
8.2-7 BSV Module with BSV that Uses Air Cylinders, Front View (See
Figure 8.2-7), 8.2-13
8.2-8 BSV Module with BSV that Uses an Actuator, Front View (See
Figure 8.2-8), 8.2-15
8.2-9 BSV Module, Rear View (See Figure 8.2-9), 8.2-17
8.2-10 BSV Module, Ball and Slide Assembly (See Figure 8.2-10), 8.2-19
8.2-11 Pump Module, Old Configuration (See Figure 8.2-11), 8.2-21
8.2-12 Pump Module, New Configuration (See Figure 8.2-12), 8.2-23
8.2-13 Rotary Cap-Pierce Module (See Figure 8.2-13), 8.2-25
8.2-14 Rotary Cap-Pierce Module, Carousel Assembly (See Figure 8.2-14), 8.2-27
8.2-15 Autoloader Module, Front View (See Figure 8.2-15), 8.2-29
8.2-16 Autoloader, Rear View (See Figure 8.2-16), 8.2-31
8.2-17 Autoloader Module, Rocker Bed Sensors and Hardware (See
Figure 8.2-17), 8.2-33
8.2-18 Autoloader Module Needle Assembly and Sensors (See Figure 8.2-18), 8.2-35
8.2-19 Triple Transducer Module (See Figure 8.2-19), 8.2-37
8.2-20 Flow-Cell and Laser Assembly (See Figure 8.2-20), 8.2-39
8.2-21 Main Diluter Module (See Figure 8.2-21), 8.2-41
8.2-22 Electronic Power Supply, Top View (See Figure 8.2-22), 8.2-43
8.2-23 Pneumatic Power Supply, Left-Side View (See Figure 8.2-23), 8.2-45
8.2-24 Analyzer Module, Card Cage Components (See Figure 8.2-24), 8.2-47
8.2-25 Wire Harness Components (See Figure 8.2-25), 8.2-49
8-ii PN 4235961E
8PARTS LISTS 8
8.1 MASTER PARTS LIST
The parts listed in this section are divided into tables by category. Within each table, the part
numbers are listed in numeric order. Table 8.1-1 lists the categories and their table references.
Table 8.1-1 Part Categories
Category Table Category Table

Accessories 8.1-2 Mixing Module 8.1-13
Autoloader Module 8.1-3 Options/Upgrade Kits 8.1-14
Bar-Code System 8.1-4 Pickup Tubes and Sensors 8.1-15
BSV Module 8.1-5 Power Supplies 8.1-16
CBC Module 8.1-6 Pump Module 8.1-17
Circuit Cards 8.1-7 Rotary Cap-Pierce Module 8.1-18
Covers/Doors 8.1-8 Shielded Reagent Lines 8.1-19
Fuses 8.1-9 Software 8.1-20
Interconnect Cables 8.1-10 Tools and Supplies 8.1-21
Main Diluter Module 8.1-11 Triple Transducer Module 8.1-22
Miscellaneous Hardware 8.1-12 Tubing 8.1-23
Table 8.1-2 Accessories
Part Number Description Figure Item

4004269 Battery pack, 376 CPU
4836885 IC Controller, DMA/Bus/Interrupt
4836887 IC, memory, RAM
6913038 Minimum support kit, customer accessories kit
7000364 Neck support, cube, with wire tie
PN 4235961E 8.1-1
PARTS LISTS
MASTER PARTS LIST
Table 8.1-3 Autoloader Module

1021292 Index motor hub 8.2-15 19
1021293 Pivot-link drive 8.2-15 14
1021296 Pin, pivot 8.2-17 1
1021300 Washer, needle home flag
1021301 Pin, actuator, needle home flag
1021302 Spring, index finger
1021303 Return spring, cone sensor
1021311 Actuator body, cone sensor 8.2-15 6
1021392 Spring, index ramp
1021393 Plate, motor mount 8.2-15 7
1021412 Actuator sensor, cone sensor 8.2-15 3
1021569 Cassette shelf spring 8.2-15 5
1021597 Stud, locking, code wheel
1021599 Lock wheel, rocker bed 8.2-15 9
1021600 Wheel, cassette shelf 8.2-15 1
1021601 Roller, cassette shelf 8.2-15 2
1021634 Guide, cassette platform 8.2-17 3
1021655 Bellows, needle 8.2-18
1021727 Spring, latch cassette
1022099 Bellows, retainer 8.2-18 10
1022161 Guide, cassette bed 8.2-17 7
1022191 Clip, retaining, cassette position opto switch
1022376 Tube, forward sensor spring arm
1022462 Tube, forward sensor arm 8.2-18 7
1022607 Tube-ram insert (button)
2429923 Label, bar-code, cassette sequencing #
2523682 Bushing, flanged, for rocker bed
2523683 Rod bearing, right 8.2-15 13
2523684 Rod bearing, left 8.2-15 15
2523693 Spring, needle home flag
2804083 Screw, rocker motor hub 8.2-15 17
2851227 Screw, index hub shoulder 8.2-15 20
2851231 E-clip, index finger
2851702 Holder, LED
2851722 Tip, tube ram (use with tube-ram insert [button] PN 1022607) 8.2-15 4
8.1-2 PN 4235961E
PARTS LISTS
MASTER PARTS LIST 8
Table 8.1-3 Autoloader Module (Continued)

2851892 Washer, spring 8.2-17 10
4836872 LED, green
5104102 Switch, STOP
6027284 Clamp, wire tie
6028262 Cable, ground, 8 in.
6028263 Cable, ground, 13 in., E53 to E18
6028264 Cable, ground, 6 in., E18
6028267 Cable, ground, 16 in., E53 to E25
6028385 Cable, ribbon, flat, Autoloader 8.2-16 5
6232208 Fitting, elbow, code wheel cylinder
6232214 Fitting, elbow, code wheel cylinder
6232376 Solenoid valve, SMC, 5-way 8.2-16 2
6232568 Solenoid manifold, SMC, dual bank 8.2-16 7
6232597 Cylinder, tube ram 8.2-16 6
6232601 Cylinder, tube return 8.2-18 8
6232620 Cylinder, needle 8.2-18 6
6232999 Cylinder, air, with magnet 8.2-18 12
6605294 Cassette, 16 mm (one each)
6605295 Cassette kit, 10/13 mm (five each)
6805407 Shield, bar-code scanner
6806247 Sensor, Hall effect (for Autoloader module only) 8.2-18 11
6858381 Encoder disk 8.2-15 8
6858383 Link drive 8.2-15 22
6858390 Index finger
6858408 Flag, lower platform
6858413 Slide, rack ball 8.2-16 4
6858474 Hub, pivot, rocker motor 8.2-15 16
6858476 Dead plate 8.2-18 1
6858478 Flange, pivot, 90 degree
6858494 Full cassette index rotation sensor and harness, 13 in. 8.2-17 8
6858496 Tube available sensor and harness, 7 in. 8.2-17 6
6858498 Unload (left) elevator down sensor and harness 8.2-17 9
6858499 Tube-ram sensor and harness 8.2-16 1
6858501 Interlock door switch 8.2-15 21
6858502 Harness, STOP switch, to door
PN 4235961E 8.1-3
PARTS LISTS
MASTER PARTS LIST
Table 8.1-3 Autoloader Module (Continued)

6858503 Harness, STOP switch, on door
6858504 Motor, rocker bed
6858505 Motor, cassette, lift 8.2-15 12
6858506 Index motor, cassette 8.2-15 18
6858507 Sensor and harness, 15 in. (tube forward, needle forward, and needle home sensors 8.2-15 10
on Configuration A, tube forward sensor on Configuration B ) 8.2-18 9
6858609 Load (right) elevator down sensor and harness 8.2-17 5
6858739 Sensor and harness, cassette 0 and 3 8.2-17 2
6858741 Sensor and harness, cassette 1 and 2 8.2-17 4
6858742 Bracket, lower door
6859238 Body front support
6859268 Guide, needle slide 8.2-18 3
7000201 Bracket, needle cartridge driver 8.2-18 5
7000447 Needle cartridge 8.2-18 2
7000472 Autoloader module 8.2-15
7000639 Rocker bed assembly 8.2-17
7000651 Bed lock assembly 8.2-15 11
9908617 E-clip, 90 degree, pivot flange
Table 8.1-4 Bar-Code System

2016197 Holder, bar-code wand
2016512 Interface, smartwand, 115 Vac, Minerva box
2016513 Smartwand, medium resolution
2016515 Interface, smartwand, 220 Vac, Minerva box
6915312 Bar-code wand, Welch Allyn, Universal
7000041 Card, Bar-Code Reader Decoder
7000042 Laser bar-code reader 8.2-13 4
8.1-4 PN 4235961E
PARTS LISTS
MASTER PARTS LIST 8
Table 8.1-5 BSV Module

1016788 Insulator (for BSV that uses air cylinders) 8.2-7 6
1017361 Shaft, BSV, coated (for BSV that uses air cylinders) 8.2-7 7
1020163 Retainer, tube (lower ball slide) 8.2-10 12
1020521 Stop, ball slide 8.2-10 9
1020525 Spacer, rinse block support 8.2-10 17
1020527 Rinse block arm, secondary 8.2-7 12
8.2-10 18
1020529 Retainer, tube (outer ball-slide stop) 8.2-10 10
1020539 Retainer, tube (inner ball-slide stop) 8.2-10 8
1024398 Bushing, shaft (use with BSV actuator PN 6232921) 8.2-8 13
2512031 O-ring, 0.375 i.d. x 0.5 o.d. (seals BSV housing - for BSV that uses air cylinders)
2515004 Spring, fxt, 0.310 d (ball-slide return) 8.2-10 24
2523062 O-ring, 0.187 i.d. x 0.050 w 8.2-8 7
2527868 Knob (use with BSV actuator PN 6232994) 8.2-8 11
2527869 Bushing, shaft (use with BSV actuator PN 6232994) 8.2-8 10
2527870 Bracket, left section (pad) segmenting (use with BSV actuator PN 6232994) 8.2-8 12
2527871 Bushings, centering (use with BSV actuator PN 6232994) 8.2-8 9
2804005 Screw, pan head, #4-40 x 0.25 (for lower tube retainer, rinse block arm) 8.2-10 15
2804011 Screw, machine, #4-40 x 0.25, flat82-HD (for rinse block arm) 8.2-10 16
2804074 Screw, machine, #4-40 x or 0.31, flat82-HD (for ball-slide stop) 8.2-10 11
2804083 Screw, hex, #4-40 x 0.25 (for ball slide) 8.2-10 7
2806090 Screw, pan head, #6-32 x 0.50 (for CL3) 8.2-10 3
2822030 Nut, hex, #6-32 unc x 0.114 thick (for CL3 bracket) 8.2-10 6
2823550 Bearing, ball slide 8.2-10 25
2826002 Washer, split lock #4 (for lower tube retainer) 8.2-10 14
2826035 Washer, split lock, #6, 0.14 i.d. x 0.25 o.d. x 0.031 thick (for CL3 bracket) 8.2-10 5
2827087 Washer, flat, vinyl (for rinse block) 8.2-7 10
8.2-8 16
8.2-10 20
2827133 Washer, flat, #4 (for lower tube retainer) 8.2-10 13
2827134 Washer, flat, #6, 0.56 i.d. x 0.375 o.d. x 0.046 thick (for CL3 bracket) 8.2-10 4
2851460 Shoulder washer 8.2-7 5
2851915 Screw, shoulder, rinse block, securing 8.2-7 11
8.2-8 17
8.2-10 21
2851918 Setscrew, 6-32, rinse block, adjustment 8.2-10 2
PN 4235961E 8.1-5
PARTS LISTS
MASTER PARTS LIST
Table 8.1-5 BSV Module (Continued)

6027284 Clamp, wire tie
6232277 Fitting, 0.093 i.d. 8.2-8 7
6232357 Cylinder, air pot, backwash arm (CL3) 8.2-10 22
6232921 Actuator, valve, double acting, dual concentric shafts, and BSV cylinder knob and 8.2-8 8
bracket (configuration A actuator)
6232994 Actuator, valve, double acting, dual concentric shafts, and BSV cylinder knob and 8.2-8 8
bracket (configuration B actuator)
6707096 BSV, self cleaning, 3 sections (pads) 8.2-7 14
8.2-8 18
6806708 Sample (start) bar, Secondary mode 8.2-7 8
8.2-8 14
6855617 Knob, BSV (for BSV that uses air cylinders) 8.2-7 13
6857096 Pinch valve, single 8.2-7 3
8.2-8 3
6857904 Harness, BSV module, P51, J60, J75 8.2-9 1
6857953 Bracket, cylinder mounting (CL3) 8.2-10 1
6858031 Ball slide extension 8.2-10 23
6859004 Cylinder, air, BSV front and center (CL1 and CL2) (for BSV that uses air cylinders) 8.2-7 4
6859155 Housing, BSV panel (for BSV that uses air cylinders) 8.2-7 7
6859138 Foam trap, 3-port (FMT4) 8.2-8 23
6915200 BSV module (with BSV that uses an actuator) 8.2-8
7000034 Pump, calibrated, air, 0.064 mL 8.2-7 2
8.2-8 5
7000035 Tank, sheath 8.2-7 1
8.2-8 4
7000037 Peltier module 8.2-9 2
7000074 Blood detectors, matched set 8.2-8 19
7000078 Pump, Primary-mode aspiration, 0.185 mL 8.2-8 1
7000079 Switch, rinse block, position detect
7000082 Rinse block 8.2-7 9
8.2-8 15
8.2-10 19
7000104 Fan and toroid, Peltier cell 8.2-9 4
7000138 Pump, Secondary-mode aspiration, 0.125 mL 8.2-8 2
7000146 Ambient Temperature Sensor 2 card
8.1-6 PN 4235961E
PARTS LISTS
MASTER PARTS LIST 8
Table 8.1-6 CBC Module

1013756 Tube, optical
2512088 Seal, O-ring (top of aperture module to fittings)
2512104 O-ring, aperture, large
2512120 O-ring, aperture, small
2815002 Thumbscrew, stainless steel
2830012 Grommet, small 8.2-2 14
6232342 Solenoid, Clippard, mixing bubble 8.2-3 4
6232443 Quick disconnect, male 8.2-3 2
6232579 Valve, Angar™ 8.2-2 15
6702275 Aperture, WBC
6702720 Aperture, RBC
6706772 Sensor and housing, overflow, bath, fused 8.2-3 3
6853869 Pumps, lytic reagent, matched set, 1.04 mL total 8.2-2 13
6854732 Chamber, isolator (VC1) 8.2-2 12
6855858 Shield, sweep-flow line
6856916 Manifold, bath bubble assembly (mixing bubbles) 8.2-3 6
6857096 Pinch valve, single
6857903 Harness, CBC module, P54 8.2-3 5
6859138 Foam trap, 3-port (FMT3, VC2, VC10, VC11) 8.2-2 5
8.2-3 9
6915223 CBC module 8.2-2
7000051 Hgb Preamp 8.2-2 6
6915174 Lamp, aperture, package of two 8.2-2 7
7000085 Mirror, top, adjustable 8.2-3 11
7000109 Chamber, overflow (VC8) 8.2-2 10
7000113 Canister, sweepflow 8.2-3 10
7000114 RBC/WBC bath and Aperture module with cable 8.2-2 9
7000429 Hgb lamp with bracket 8.2-2 8
PN 4235961E 8.1-7
PARTS LISTS
MASTER PARTS LIST
Table 8.1-7 Circuit Cards

6705330 Rocker Bed Interface card
6705331 Autoloader Interface card (non-CE) 8.2-16 3
6705746 RCP Junction II card 8.2-13 1
6705768 Fluid Detector/Ram Pressure card 8.2-21 1
6705938 Retic Interface card, and harness (P109) 8.2-25 13
6706413 Waste Level Sense card
6706469 Autoloader Interface card (CE) 8.2-16 3
6706944 Ready/Stop LEDs card for instruments with an Autoloader module (mounted on rear
of upper front door.)
6706945 Ready/Stop LEDs card for instruments with a Rotary Cap-Pierce module (mounted on
rear of upper front door)
6858109 Card cage, with backplanes 8.2-24 7
7000010 Backplane Solenoid Junction card
7000020 Pneumatic Monitor card 8.2-21 2
7000021 Sample Handler I card
7000023 I/O card 8.2-24 3
7000024 DILUTER INTERFACE card 8.2-24 2
7000025 R/W Preamp card 8.2-24 6
7000026 376 CPU card 8.2-24 1
7000027 R/W/P PROC card 8.2-24 5
7000028 VCS PROCESSOR card 8.2-24 4
7000029 RCP I Junction card
7000036 RAM Timer card, 376
7000041 Bar-Code Reader Decoder card
7000087 Peltier Controller card 8.2-9 3
7000123 Overflow Sensor Interface card
7000146 Ambient Temperature card
7000172 Sample Handler II card
8.1-8 PN 4235961E
PARTS LISTS
MASTER PARTS LIST 8
Table 8.1-8 Covers/Doors

1022498 Guard, switch, lower door
1024764 Bracket, magnetic mount
2851480 Screw, shoulder (for molded side panel)
5111192 Magnet, door
5120211 Switch, rocker, lower front door (Standby/Reset)
6705812 Door, upper front
6805411 Door, upper front
6805414 Door, lower rear Diluter (CE)
6805608 Panel, decorative, front right, on instruments with an Autoloader module (CE)
6805633 Cover, card cage, access (CE)
6806738 Support, catch
6806749 Slide rack, ball bearing, lower front door
6857831 Side panel, molded
6858055 Door, lower, on instruments with a Rotary Cap-pierce module
6858337 Plate, magnet mounting
Table 8.1-9 Fuses

5102011 Fuse, 0.3 A (F9 - 220/240 Vac)
5102012 Fuse, 1.0 A (F5, F6 - 220/240 Vac)
5102014 Fuse, 6.25 A (F1, F2 - 100/120 Vac)
5102016 Fuse, 0.5 A (F3 - 100/120 Vac)
5102018 Fuse, 4.0 A (F4 - 100/120 Vac)
5102021 Fuse, 2.0 A (F4 - 220/240 Vac, F5/F6 - 100/120 Vac)
5102026 Fuse, 0.25 A (F3 - 220/240 Vac, F8 - 100/120 Vac)
5102029 Fuse, 1.5 A (F10 - 220/240 Vac) (Pneumatic Power Supply)
5102043 Fuse, 0.0625 (1/16) A (F7 - 220/240 Vac)
5102058 Fuse, 0.125 A (F7 - 100/120 Vac, F8 - 240 Vac)
5102084 Fuse, 0.4 A (F9 - 100/120 Vac)
5120184 Fuse, 2.5 A (F10 - 100/120 Vac) (Pneumatic Power Supply)
9908780 Fuse, 3.2 A (F1, F2 - 220/240 Vac)
PN 4235961E 8.1-9
PARTS LISTS
MASTER PARTS LIST
Table 8.1-10 Interconnect Cables

6027225 Cable, Electronic Power Supply to peripherals
6027903 Cable, power, EPS to peripherals (CE)
6027923 Cable, RF Detector Preamp card to backplane (P73) 8.2-25 11
6028130 Cable, solenoid with connector, two conductor
6028235 Cable, bar-code scanner to Bar-Code Reader Decoder card
6028236 Cable, sample handler to auto-piercer
6028238 Cable, ribbon, sample handler to bar-code reader
6028274 Cable, LS Preamp module to backplane (P78 to P49) 8.2-25 12
6028275 Cable, communication, to bar-code decoder
6028463 Cable, external waste level sensor
6028480 Cable, LS offset monitor (P113 to P7 [A13 and B38]) 8.2-25 14
6028504 Cable, parallel printer, 10 ft (CE)
6028711 Cable, P2, serial Printer
6028712 Cable, P4, bar-code wand
6028714 Cable to MAXM analyzer J1, Digi Breakout Box P1
6028717 Cable, Digi Breakout Box
6705938 Harness, Retic Interface, and card (P109) 8.2-25 13
6806369 Harness, main wiring
6806371 Harness, interconnect, J50, J54, J51, J53, J52
6857192 Cable, flow cell to RF box
6857900 Harness, Mixing Chamber module, P52 and J71 8.2-5 3
6857902 Harness, Main Diluter module, P50 to J81
6857903 Harness, CBC module, P54 8.2-3 5
6857904 Harness, BSV module, P51, J60, J75 8.2-9 1
6857905 Harness, Pump module, P53 8.2-11 12
8.2-12 9
6858502 Harness, STOP switch, to door
6858503 Harness, STOP switch, on door
6858931 External harness, level sense float
7000060 Cable assembly, com 4, Digiboard comm
8.1-10 PN 4235961E
PARTS LISTS
MASTER PARTS LIST 8
Table 8.1-11 Main Diluter Module

6232081 Tank, air (high vacuum/60-psi pressure)
6232443 Quick disconnect, male
6232444 Quick disconnect, female
6806053 Pump, backwash, 3 mL (PM8) 8.2-21 10
6854671 Manifold, 30 psi (MF14) 8.2-21 11
6854732 Chamber, isolator (VC1, VC5) 8.2-21 20
6857902 Harness, Main Diluter module, P50 to J81
6905043 Dispenser, RBC, 10 mL 8.2-21 21
6912099 Dispenser, WBC, 6 mL 8.2-21 18
7000055 Choke, sheath, #2, reg (CK30) 8.2-21 16
7000064 Pump, dispense, 5 mL (PM10, cleaning agent) (PN 6806058) 8.2-21 17
7000067 Water trap, compressor (FL2) 8.2-21 13
7000068 Water trap, vacuum (FL1) 8.2-21 12
7000069 Regulator, 30 psi (RG2) 8.2-21 15
7000070 Solenoid, condensate drain (LV43) 8.2-21 14
7000071 Regulator, sample/sheath pressure (RG3, RG4) 8.2-21 8
7000145 Regulator, vacuum, solid state (RG1) (6232628) 8.2-21 19
7000380 Chamber, isolator (VC6, VC7) 8.2-21 7
7000606 Optical reagent sensor and housing (diluent and cleaning agent) 8.2-21 6
7000607 Optical reagent sensor and housing (lytic reagent and PAK) 8.2-21 5
7000650 Bracket, dual tank, with air tanks
PN 4235961E 8.1-11
PARTS LISTS
MASTER PARTS LIST
Table 8.1-12 Miscellaneous Hardware

1004595 Fitting, hose, polyurethane, 0.062 i.d.(used in Aperture module)
1004645 Fitting, plug, stopper
1005693 Fitting, hose, stainless steel, 0.062 diameter
1005699 Fitting, feed-through, 0.062 i.d., standard 8.2-2 2
8.2-5 1
1016486 Isolator, feed-through fitting 8.2-2 1
8.2-4 9
1016929 Fitting, feed-through, small i.d.
1017501 Mount, pinch valve 8.2-2 3
8.2-5 6
8.2-8 21
1018245 Fitting, Y, large. 0.085 i.d.
1808055 Ferrite EMI suppressor, 0.390 in. diameter
2014361 Connector, 3-conductor (J106 for TTM) 8.2-25 8
2104189 Contact, pin, 24-20 8.2-26 1
2104190 Contact, socket 8.2-26 2
2104208 Clamp, connector, circular (J50) 8.2-25 1
2104258 Clamp, connector, circular (J26, J80, J82) 8.2-25 16, 18
2104318 Contact, socket, 24-20 AWG, square post (P66 for LS Preamp module, P79, P104 for 8.2-25 4, 9, 10
RF Detector Preamp card) 8.2-26 5
2104334 Clamp, connector, circular (J51, J52, J53, J54) 8.2-25 15
2104339 Connector, circular, 8-pin (J80, J82) 8.2-25 18
2104347 Connector, 15-conductor, universal Mat-N-LK (J32 for instruments with float reagent 8.2-25 6
sensors)
2104348 Pin, connector, universal Mat-N-LK, 24-18 AWG, brass 8.2-26 3
2104349 Socket T, connector, universal Mat-N-LK, 24-18 AWG, brass 8.2-26 4
2104351 Connector, nine conductor, universal Mat-N-LK, female (J69 for TTM) 8.2-25 7
2104357 Connector, two conductor, universal Mat-N-LK (J32 for instruments with optical 8.2-25 5
reagent sensors, and J77)
2104360 Connector, 3-position, male (P5 for Peltier module) 8.2-25 17
2104418 Holder, socket, crimp terminal, 24-18 AWG, Molex 8.2-26 7
2113010 #8 ring, 22-16 AWG, insulated 8.2-26 9
8.1-12 PN 4235961E
PARTS LISTS
MASTER PARTS LIST 8
Table 8.1-12 Miscellaneous Hardware (Continued)

2113045 Terminal receptacle, 22-18 AWG 8.2-26 10
2113052 Terminal, solderless, #5 spring spade, 22-16 AWG 8.2-26 6
2121280 Connector (P91) 8.2-25 3
2121328 Connector, 3-position (P66 for LS Preamp module) 8.2-26 10
2121415 Connector, 4-position (P104 for RF Detector Preamp card) 8.2-25 9
2121420 Terminal, solderless receptacle, 90 degree, 22-18 AWG 8.2-26 8
2121421 Contact, socket, 26-22 AWG, square post 8.2-26 5
2121425 Fifteen-position assembly module (J32 for instruments with float reagent sensors) 8.2-25 6
2121426 Nine-position assembly module (J69 for TTM) 8.2-25 7
2121436 Adapter for cap housing (J32, for instruments with float reagent sensors) 8.2-25 6
2121436 Adapter (J69 for TTM) 8.2-25 7
2121442 Connector (P1, P4 for Peltier module) 8.2-25 2
2121454 Connector (P79) 8.2-25 4
2121684 Connector, circular, 28-pin (J51, J52, J53, J54) 8.2-25 15
2121685 Connector, circular, 8-pin (J26) 8.2-25 16
2121687 Connector, circular, 57-pin (J50) 8.2-25 1
2512007 O-ring, 0.687 i.d.
2512064 O-ring, 0.25 i.d. x 0.375 o.d.
2523608 Gasket, SMC solenoid
2523616 Latch, magnetic
2603009 Grill, fan, 4 in.
2603010 Filter, fan
2851121 Strike plate, steel, self-adhesive
2851784 Screw, SMC solenoid
2851821 Grommet, black plastic (decorative panel)
2851822 Plunger, black plastic (decorative panel)
2852004 Clamp, plastic, 60 psi line
5116004 Switch, magnetic reed (door interlock)
6011001 Wire tie, 4 in.
6011018 Wire tie, 8 in.
6211015 Actuator, pilot, small 8.2-3 1
8.2-5 5
8.2-8 20
6213006 Choke, pneumatic, red (0.008 orifice)
PN 4235961E 8.1-13
PARTS LISTS
MASTER PARTS LIST
Table 8.1-12 Miscellaneous Hardware (Continued)

6213008 Choke, pneumatic, yellow (0.004 orifice)
6213009 Choke, pneumatic, brown (0.006 orifice)
6213010 Choke, pneumatic, blue (0.012 orifice)
6213011 Choke, pneumatic, black (0.010 orifice)
6213016 Choke, variable, 1/16 i.d. (for pressure line of CBC lytic reagent pumps)
6214106 Check valve, large
6214107 Check valve, medium
6214108 Check valve, small
6216354 Fitting, T, large, 0.145 i.d.
6216357 Fitting, nylon, insert locking
6232036 Fitting, 0.036, 10-32 standard 8.2-5 4
6232051 Fitting, T, small, 0.094 i.d.
6232075 Solenoid valve, 3-way, 50 psi, 24 Vdc 8.2-8 6
6232259 Fitting, Y, small. 0.093 i.d.
6232352 Fitting, reducer, 0.062 i.d. x 0.093 i.d.
8.2-16 2
8.2-21 4
8.2-6 2
6232447 Actuator, pilot, large
6232564 Fitting, union, 0.125 to 0.062 i. d.
6232605 Check valve, extra large (rear of Main Diluter module, diluent/cleaning agent lines)
6855763 Pinch valve, double acting, I-beam, removable, standard 8.2-2 4
8.2-5 9
8.2-8 22
6856319 Pinch valve, triple 8.2-3 8
8.2-21 9
6856502 Plate assembly, quick disconnect
6857096 Pinch valve, single, miniature, round 8.2-4 5
8.2-7 3
8.2-8 3
6859138 Foam trap, 3-port (FMT1, FMT2, FMT3, FMT4, VC2, VC10, VC11) 8.2-8 23
8.2-21 3
8.1-14 PN 4235961E
PARTS LISTS
MASTER PARTS LIST 8
Table 8.1-13 Mixing Module

1019262 Shaft, eccentric
2523221 Belt, mixing motor 8.2-5 2
2523521 Timing pulley, 60 grooves
2523522 Timing pulley, 10 grooves
2827122 Washer, Teflon
6232434 Ring, Luer lock, white (use with PN 6232435 and PN 6232436) 8.2-4 3
6232435 Fitting, Luer, 0.094 i.d. male
6232436 Fitting, Luer, 0.094 i.d. female 8.2-4 2
6232443 Quick disconnect, male
6805583 Drive assembly, mixing motor
6857019 Holder, mixing chamber 8.2-4 8
8.2-5 7
6857900 Harness, Mixing Chamber module, P52 and J71 8.2-5 3
6858748 Mixing chamber assembly 8.2-4 6
6915226 Mixing Chamber module 8.2-4
7000032 Pump, StabiLyse reagent, 0.133 mL 8.2-4 7
7000474 Mixing chamber drive assembly 8.2-5 8
7000487 Motor, mix chamber, 24 Vdc 8.2-6 1
PN 4235961E 8.1-15
PARTS LISTS
MASTER PARTS LIST
Table 8.1-14 Options/Upgrade Kits

6913390 Options Kit, Retic key disk and accessories
6913391 Kit, Retics, hardware and computer accessories
6913393 Kit, Retic, hardware and accessories
6915018 Mod Kit, Autoloader upgrade
6915296 Kit, international, MAXM analyzer/ MAXM analyzer with Autoloader, 8DJ software?
6915297 Kit, complete MAXM analyzer 8DJ software installation (must use if software is 8BJ or
lower)
6915317 Kit, MAXM analyzer 8C to 8D software (US and Canada)
6915318 Kit, MAXM analyzer 8C to 8D software (international)
6915319 Kit, MAXM analyzer 8CJ to 8DJ software (use only if current software revision 8CJ)
Table 8.1-15 Pickup Tubes and Sensors

6600964 Pickup tube, for 500-mL and 1-L bottle (use with float sensor)
6601190 Pickup tube, with float sensor, lytic reagent for 5-L cube (use with float sensor)
6706295 Pickup tube, universal, for diluent and cleaning agent,10 L and 20 L
6706296 Pickup tube, universal, for PAK LYSE, 5 L lytic reagent, 5 L cleaning agent
6706297 Pickup tube, universal, StabiLyse, 1 L and 500 mL lytic reagent
7000007 Level sensor and pickup tube, cleaning agent (float sensor)
7000008 Level sensor and drop tube, waste (float sensor)
7000008 Waste, pickup tube with level sense
7000013 Level sensor and pickup tube, diluent (float sensor)
7000089 Pickup tube, Erythrolyse II (uses with float sensor)
7000092 Pickup tube, StabiLyse/lytic reagent (use with float sensor)
8.1-16 PN 4235961E
PARTS LISTS
MASTER PARTS LIST 8
Table 8.1-16 Power Supplies

2104348 Pin, male (used on power supply connectors)
2104349 Pin, female (used on power supply connectors)
2104353 Connector, 12-pin, female (power supply J116/J108)
2104361 Connector, 3-pin, female (power supply J113/J114)
2523590 Mount, compressor shock, 6 lb 8.2-23 4
2602022 Fan, 230 Vac (use with Pneumatic Power Supply module, 220/240 Vac) 8.2-23 2
2602023 Fan, 115 Vac (use with Pneumatic Power Supply module, 100/110 Vac) 8.2-23 2
2727758 Filter, input line
4510012 Electronic Power Supply module relay, K1 and K2
5120173 Switch, rocker (used with Electronic Power Supply)
6208005 Regulator, 60 psi 8.2-23 3
6232657 Pump, vacuum/pressure, 100/115 Vac 8.2-23 1
6232658 Pump, vacuum/pressure, 220/230 Vac 8.2-23 1
7000000 Power supply, +24 Vdc (PS4) 8.2-22 6
7000001 Power supply, +5 Vdc, 5 A (PS7, 5.6 V supply) 8.2-22 5
7000002 Power supply, +12 Vdc, 6.3 A (PS6) 8.2-22 7
7000003 Power supply, +5 Vdc, 12 A ±15 Vdc (PS3) 8.2-22 4
7000004 Electronic Power Supply module, 100 Vac (non-CE) 8.2-22
7000005 Electronic Power Supply module, 120 Vac (non-CE) 8.2-22
7000006 Electronic Power Supply module, 220/240 Vac (non-CE) 8.2-22
7000043 Aperture Voltage/Power Fail Detect card, 100 Vac 8.2-22 2
7000046 Power supply, RF 8.2-22 3
7000061 Pneumatic Power Supply module, 100/110 Vac 8.2-23
7000062 Pneumatic Power Supply module, 220/240 Vac 8.2-23
7000066 Fan, Electronic Power Supply, Toroid, 24 Vdc 8.2-22 1
7000072 Laser Power Supply, 120 Vac
7000073 Laser Power Supply, 220/240 Vac
7000120 Laser power supply, 100 Vac
7000122 Pneumatic Power Supply module, 100 Vac 8.2-23
7000462 Electronic Power Supply module, 100 Vac (CE) 8.2-22
7000463 Electronic Power supply module, 120 Vac (CE) 8.2-22
7000464 Electronic Power Supply module, 220/240 Vac (CE) 8.2-22
7231079 Compressor rebuild kit
PN 4235961E 8.1-17
PARTS LISTS
MASTER PARTS LIST
Table 8.1-17 Pump Module

2830012 Grommet, small 8.2-11 7
8.2-12 5
2830017 Grommet, large
8.2-12 6
8.2-12 7
6232443 Quick disconnect, male 8.2-11 1
8.2-12 1
6854848 Chamber, isolator (VC4 with screen) 8.2-11 10
8.2-12 8
6857905 Harness, Pump module, P53 8.2-11 12
8.2-12 9
8.2-12 2
6915224 Pump module (new configuration) 8.2-12
7000063 Pumps, matched set, Erythrolyse II reagent 8.2-11 4
8.2-12 4
7000078 Pump, Primary-mode aspiration, 0.185 mL 8.2-11 6
7000138 Pump, Secondary-mode aspiration 0.125 mL 8.2-11 5
7000381 Chamber, isolator (VC3 with screen) 8.2-11 12
8.2-12 10
8.1-18 PN 4235961E
PARTS LISTS
MASTER PARTS LIST 8
Table 8.1-18 Rotary Cap-Pierce Module

1014581 Spring, holder 8.2-14 12
1014599 Hinge block (saddle guide) 8.2-14 16
1014725 Spacer, tube clamp to air cylinder 8.2-14 17
1019604 Bracket, magnetic switch 8.2-13 22
1020044 Bracket, code-wheel sensor 8.2-14 7
1020046 Retainer, bellows 8.2-14 11
1020047 Collar, bellows 8.2-13 16
1020054 Code wheel 8.2-14 9
1020229 Bracket, dual opto, needle position 8.2-13 11
1020230 Saddle guide (hinge block, PN 1014599, pin PN 2831012) 8.2-14 14
1020232 Bellows, needle 8.2-13 18
1020390 Tube ejector (spacer two each, PN 2843056) 8.2-14 10
1020569 Screw, special 8.2-13 6
1020571 Shaft, stub, threaded 8.2-14 22
1020576 Guide, lower, tube clamp 8.2-14 18
1020577 Guide, upper, tube clamp 8.2-14 20
1020588 Lock, needle 8.2-13 7
1020594 Needle slide 8.2-13 17
1020596 Clip, needle retainer 8.2-13 8
1020644 Slide (clip), bellows 8.2-13 18
1020664 Washer, special, vibration insulator for vial guide
1020694 Spring, tube available sensor 8.2-14 5
1020745 Vial guide, molded plastic, covers half of carousel shaft 8.2-14 25
1020950 Cradle guide 8.2-14 2
1022197 Bracket, tube clamp, without bumper
2515017 Spring, compression, cap-piercer, tube retainer 8.2-14 13
2523199 Coupling, flex shaft 8.2-14 23
2523615 Shock mount for carousel motor
2523616 Latch, magnet 8.2-13 22
2804070 Screw, bar-code scanner bezel and window
2826035 Washer, split- lock, bar-code scanner bezel and window
2830029 Grommet, black trim 8.2-13 2
2831012 Pin, dowel (saddle guide) 8.2-14 15
2838063 Holder, panel, grommet for LED 8.2-14 6
2851768 Pin, pivot, tube available sensor 8.2-14 3
PN 4235961E 8.1-19
PARTS LISTS
MASTER PARTS LIST
Table 8.1-18 Rotary Cap-Pierce Module (Continued)

2851828 Washer, spring (two needed) 8.2-13 9
3213199 Bumper, rubber, tube clamp 8.2-14 21
6232376 Solenoid, 5-way 8.2-13 20
6232460 Cylinder, air, tube clamp (CL4)
6232461 Cylinder, air, needle pierce (CL5) 8.2-13 12
6232999 Cylinder, air, with magnet 8.2-13 13
6805584 Sensor, Hall effect (for Rotary Cap-Pierce module only) 8.2-13 14
6806729 Harness, cap-pierce solenoid
6857787 Lever, tube available sensor 8.2-14 4
6857947 Harness, cap-pierce front ready/standby LED
6857949 Harness, opto (includes all sensors) 8.2-13 5
6857987 Bracket, carousel, black 8.2-14 8
6857991 Needle slide with opto sensor actuator 8.2-13 10
6858029 Nut plate (fits on motor vial guide side of carousel) 8.2-14 24
6858040 Carrier, tube holder (setscrews PN 2807080) 8.2-14 1
6858111 Tray, tube catch, removable 8.2-13 19
6858141 Bracket, cradle guide
6858230 Bezel and window (for bar-code scanner)
6859030 Bracket, tube clamp with bumper 19
6915187 Rotary Cap-Pierce module 8.2-13
7000042 Laser bar-code reader 8.2-13 4
7000056 Needle, pierce, and bellows assembly 8.2-13 18
7000057 Motor, carousel (order four each 2523615 elastic shock mounts) 8.2-13 21
7000080 Transistor, photoelectric, Q1 and Q2, WM11 and WM13
7000081 Diode, light emitting, DS1 and DS2, WM10 and WM12
7000088 Needle assembly, customer replaceable (QD4) 8.2-13 18
7000363 Needle assembly, customer replaceable (needle, bellows, collar, slide, and Luer 8.2-13 18
fittings), FRU
9904647 Quick disconnect, male (QD4)
9904678 Quick disconnect, female (QD4)
9904854 Gasket, rubber, quick disconnect
8.1-20 PN 4235961E
PARTS LISTS
MASTER PARTS LIST 8
Table 8.1-19 Shielded Reagent Lines

7000091 Input line, cleaning agent, shielded
7000093 Input line, diluent, shielded
7000094 Input line, waste, shielded
7000148 Input line, CBC lytic reagent, Erythrolyse reagent, and StabiLyse reagent, shielded
Table 8.1-20 Software

6415109 Data Options Key diskette
6415110 Bi-directional Key diskette
6706062 PROM, Sample Handler, U4 7A/B/C
6706063 PROM, 376 CPU, U46 7A/B/C
6912924 Data options enable disk, Options Kit,
6912926 Bi-directional enable disk, Options Kit,
6915011 Retic III Enable Disk
6915273 Software Kit, MAXM-MAXM A/L Retic MR V/MI enable diskette (U.S. only), Rev 8D
6915274 Software Kit, MAXM-MAXM A/L Retic MR V/MI enable diskette (International only),
Rev 8D
6915276 Retic Enable Disk III, Rev 8D only
6915278 Software Kit, graphic print format setup diskettes, Rev 8D
6915279 Software Kit diskettes, Rev. 8D (includes graphic print format diskettes)
PN 4235961E 8.1-21
PARTS LISTS
MASTER PARTS LIST
Table 8.1-21 Tools and Supplies

1606138 Isopropyl alcohol (10 x 4 mL)
2016642 Digiboard diagnostics kit
2121422 Dark Current Test Plug
2527713 Applicator sticks (flow-cell cleaning)
4276605 MAXM System Verification Procedure (SVP) form
5111192 Magnet, bypass (to bypass lower door switch)
5401245 Gauge, horizontal/vertical (Z-axis)
5401269 Light shield, special
5402071 Tool, plastic trimmer, pot adjustment for C1
5402208 Tool, pin extractor (red/white)
5402226 IC extraction tool
5415206 Tool, pinch valve removal
5415219 Tool, tamperproof screw removal
5415364 Tool, plastic, potentiometer adjustment, 0.32 tips
5415365 Wrench, open end, 1/4 in.
5415374 Volumetric cylinder (used to check pump volumes)
5715370 Glasses, laser safety
6028454 Cable assembly, Autoloader Sensor Test card
6232457 Pipettor, vertical dispenser, 2.0 mL
6415262 Service Disk 2, 3.5 in.
6417073 Troubleshooter PC software diagnostics
6605419 Latex particles DC/RF (large)
6705822 Autoloader Sensor Test card
6857371 Latex particles, 94.3 fL (small)
6858098 Dummy load, RF Preamp
6858098 Dummy load, RBC (28 K)
6858376 Dummy load, WBC (14 K)
6912920 PAK (Erythrolyse II reagent) pump volume kit, without vial PN 5415374
6915392 Service Disk 3, 3.5 in.
8.1-22 PN 4235961E
PARTS LISTS
MASTER PARTS LIST 8
Table 8.1-22 Triple Transducer Module

1017940 Shaft, TTM, X-axis (horizontal) adjustment 8.2-20 13
1019356 Plate, TTM, flow-cell mounting (Rev. C) 8.2-20 20
1020249 Shield, magnetic (laser cover) 8.2-19 2
1022733 Bolt plate, horizontal (Rev. A)
2429637 Label, laser warning
2515158 Spring, TTM X-, Y-, Z-alignment shafts 8.2-20 9
2523590 Mount, shock, 6 lb
2806009 Screw, 0.25 long, #6 x 32, pan-head, xrec (flow-cell mount) 8.2-20 19
2807038 Setscrew, 10-32 x 0.25 long, hex (flow-cell lock down) 8.2-20 11
2808059 Screw, Z-axis lock down
2808110 Screw, 0.50 long, #8 x 32, hsc-head, hex 8.2-20 18
2808131 Screw, 2.25 l, #8 x 32, pan-head, xrec 8.2-20 3
2826048 Washer, flat, #2, 0.094 i.d. x 0.188 o.d. x 0.028 thick 8.2-20 4
2827021 Nut, 0.21 i.d. x 0.51 o.d. x 0.51
2827135 Washer, flat, #8, 0.187 i.d. x 0.438 o.d. x 0.063 thick 8.2-20 5
2827160 Washer, flat, #8, 0.188 i.d. x 0.375 o.d. x 0.045 thick, X/Y/Z alignment shaft 8.2-20 8
2851561 Washer, large, shock mount
2851566 Screw, tamperproof 8.2-19 3
2851567 Screw, threaded, 1.57 l, #10 x 56, used on Y- and Z-axes 8.2-20 7
2851568 Nut, hex, #10, used on X-axis 8.2-20 14
2851569 Nut, square, #10, used on Y- and Z-axes 8.2-20 10
2851573 Screw, Allen, TTM mounting
2851614 Screw, 0.62 long, #8 x 32, TTM lock down 8.2-20 17
2852270 Washer, lock, spring, 0.164 i.d. x 0.343 o.d. x 0.013 thick 8.2-20 16
2852276 Screw, 0.62 long, #8 x 32, hex-head, ehex 8.2-20 15
4802024 LED, red, TTM laser ON
5120035 Switch, interlock, laser
5704031 Vacuum tube, RF triode, 12AU7A (new style) 8.2-19 4
6706013 Sensor, LS, 3-element (Rev. B), for retic ready units (use with LS Preamp 5 module) 8.2-20 2
6706039 Flow cell, coated and tested 8.2-20 1
6856153 Plates, TTM, horizontal and vertical adjustment 8.2-20 11
6858361 Shield, flow cell (cover) 8.2-19 1
6859066 LS Preamp 5 module 8.2-19 7
6859777 RF Detector Preamp #6 assembly 8.2-19 5
7000031 Sensor, LS (Rev. E), on instruments without the Retic option (use with LS Preamp 3 8.2-20 2
module)
PN 4235961E 8.1-23
PARTS LISTS
MASTER PARTS LIST
Table 8.1-22 Triple Transducer Module (Continued)

7000038 Base and laser mount 8.2-19 6
7000040 LS Preamp 3 module, on instruments without the Retic option 8.2-19 7
7000551 Lens block assembly 8.2-20 6
7000646 TTM assembly, complete
8.1-24 PN 4235961E
PARTS LISTS
MASTER PARTS LIST 8
Table 8.1-23 Tubing

1018010 Tubing, special, sample line, 14.6 in. long
Routing: At CBC module, from BT2-7 to SF1
1018301 StabiLyse line (delivery to mixing chamber)
3202036 Tubing, poly, 0.082 i.d., clear
3202038 Tubing, poly, 0.113 i.d.
3202039 Tubing, poly, 0.145 i.d.
3202044 Tubing, trim, black
3202061 Tubing, PVC, red, for critical length tubings
3202203 Tubing, poly, 0.082 i.d., black/clear
3202204 Tubing, poly, 0.082 i.d., brown/clear
3202205 Tubing, poly, 0.082 i.d., red/clear
3202206 Tubing, poly, 0.082 i.d., orange/clear
3202207 Tubing, poly, 0.082 i.d., yellow/clear
3202208 Tubing, poly, 0.082 i.d., green/clear
3202210 Tubing, poly, 0.082 i.d., violet
3202211 Tubing, poly, 0.082 i.d., grey/clear
3202212 Tubing, poly, 0.082 i.d., white/clear
3202213 Tubing, poly, 0.082 i.d., brown/black
3202214 Tubing, poly, 0.082 i.d., red/black
3202215 Tubing, poly, 0.082 i.d., orange/black
3202216 Tubing, poly, 0.082 i.d., yellow/black
3202217 Tubing, poly, 0.082 i.d., green/black
3202221 Tubing, EVA, 0.062 i.d.
3202243 Tubing, poly, 0.081 i.d., violet/black
3202244 Tubing, poly, 0.081 i.d., grey/black
3202277 Tubing, poly, 0.082 i.d., black
3203026 Tubing, silicone, green striped, pinch
3213073 Tubing, silicone, 0.023 i.d., red stripe
3213136 Tubing, pull apart, black stripe
3213137 Tubing, pull apart, blue stripe
3213139 Tubing, pull apart, red stripe
3213163 Tubing, silicone, 0.062 i.d., brown stripe
3213176 Tubing, silicone, 0.079 i.d., yellow stripe
3213197 Tubing, pull apart, 0.062 i.d. x 0.031 w
3213205 Tubing, Tygon®, 0.030
PN 4235961E 8.1-25
PARTS LISTS
MASTER PARTS LIST
Table 8.1-23 Tubing (Continued)

3213212 Tubing, thermoplastic, 0.062 i.d. x 0.047 w
6857275 Quench (StabiLyse reagent) line
Routing: At Mixing module, from MC1-5 through PV53 (normally closed) to FF173-1
6857802 Secondary mode aspirator assembly tubing, with fitting
6858077 Tubing, special, 7.0 in., 0.023 i.d. 8.2-11 3
Routing: At Pump module, from FMT1-3 to CV79-2 8.2-12 3
At Main Diluter module -
r From MF9-3 to FF205-2
r From FF233-2 to CV41-2
r From FF210-2 to CV26-1
6859002 Pre-prep/LATRON control line, 6-in. long x 0.030 i.d.
Routing: At BSV module, from FT31-2 to FF29-2.
6859003 Pre-prep/LATRON control line, 18-in. long x 0.030 i.d.
Routing: From BSV module FF37-2 to Mixing module MC1-3
6859115 Tubing, special, 4.5 in. long x 0.011 i.d., sheath restrictor #1
Routing: At Mixing module, from FF176-1 to FF177-1
6859118 Tubing, line choke, 4.75 in. long x 0.021 i.d. (CBC lytic reagent restrictor)
Routing: At CBC module, from FU7-2 to FY8-2
6859267 Tubing, line choke, 7.5 in. long x 0.021 i.d. (CBC lytic reagent restrictor)
Routing: At CBC module, from FU7-2 to FY8-2
7000095 Rinse line, 4.5 in. long x 0.030 i.d.
Routing: At BSV module, from BSV WM6 to Peltier module, PLB1-2
7000096 Rinse line, 4.0 in. long x 0.030 i.d.
Routing: From BSV module Peltier module, PLB1-3, to Mixing module FF172-2
7000097 Sample line, 11.0 in long x 0.023 i.d.
Routing: From BSV module, BSV WM11, through Mixing module PV51 (normally
open) to MC1-4
7000100 Tubing, special, 6.0 in. long x 0.010 i.d.
Routing: From Mixing module MC1-7 through PV55 to Flow Cell module FC1-1
7000101 Tubing, special, 3.5 in.long x 0.010 i.d., sheath restrictor #1
Routing: At Mixing module, from FF176-1 to FF177-1
7000102 Tubing, special, 15.0 in.
8.1-26 PN 4235961E
PARTS LISTS
ILLUSTRATED PARTS 8
8.2 ILLUSTRATED PARTS
On the illustrations in this section, a number is used to identify the part in the associated
parts list, a letter to indicate detailed illustrations are available for the component or assembly.
Figure 8.2-1 Main MAXM Analyzer Components Illustrated Figure Reference

Front view Right side view A Figure 8.2-24
B G
A B Front, Figure 8.2-2
Rear, Figure 8.2-3
C Front, old configuration,
Figure 8.2-7
F Front, new configuration,
C
16 17 18 19
Figure 8.2-8
MF2
Rear, Figure 8.2-9

D Old configuration,
LASER
ON
Figure 8.2-11
MK6
MF7
H New configuration,
Figure 8.2-12
E Front, Figure 8.2-15
D
E
Rear, Figure 8.2-16
Back view I Rocker bed, Figure 8.2-17
Sensors, Figure 8.2-18
F Figure 8.2-22
G Figure 8.2-21
H Figure 8.2-19
F
I Front, Figure 8.2-4
Right-side, Figure 8.2-5
Left-side, Figure 8.2-6
J J Figure 8.2-23
5961261D
Table 8.2-1 Illustrations Not Referenced from Figure 8.2-1
Title Figure Number

Rotary Cap-Pierce Module (See Table 8.2-13) 8.2-13
Rotary Cap-Pierce Module, Carousel Assembly (See Table 8.2-14) 8.2-14
Wire Harness Components (See Table 8.2-25) 8.2-25
Electrical Connectors 8.2-26
PN 4235961E 8.2-1
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-2 CBC Module, Front View (See Table 8.2-2)
1 2
15
3
14
4
13
12
5
11 6
7
10
8
9
5961129D
8.2-2 PN 4235961E
PARTS LISTS
ILLUSTRATED PARTS 8
Table 8.2-2 CBC Module, Front View (See Figure 8.2-2)
Item Part Number Description

6915223 CBC module
1 1016486 Isolator, feed-through fitting
2 1005699 Fitting, feed-through, 0.062 i.d., standard
3 1017501 Mount, pinch valve
4 6855763 Pinch valve, double acting, I-beam, removable, standard
5 6859138 Foam trap, 3-port (VC10, VC11)
6 7000051 Hgb Preamp
7 6915174 Lamp, aperture, package of two
8 7000429 Hgb lamp with bracket
9 7000114 RBC/WBC bath and Aperture module with cable
10 7000109 Chamber, overflow (VC8)
11 2830012 Grommet, small
12 6854732 Chamber, isolator (VC1, VC5)
13 6853869 Pumps, lytic reagent, matched set, 1.04 mL total
14 2830017 Grommet, large
15 6232579 Valve, Angar™
PN 4235961E 8.2-3
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-3 CBC Module, Rear View (See Table 8.2-3)
11
10 2
9 3
5961130D 8 7 6 5
8.2-4 PN 4235961E
PARTS LISTS
ILLUSTRATED PARTS 8
Table 8.2-3 CBC Module, Rear View (See Figure 8.2-3)

1 6211015 Actuator, pilot, small
2 6232443 Quick disconnect, male
3 6706772 Sensor and housing, overflow, bath, fused
4 6232342 Solenoid, Clippard, mixing bubble
5 6857903 Harness, CBC module, P54
6 6856916 Manifold, bath bubble assembly (mixing bubbles)
7 6232412 Solenoid valve, SMC, 3-way
8 6856319 Pinch valve, triple
9 6859138 Foam trap, 3-port (FMT3, VC2)
10 7000113 Canister, sweep flow
11 7000085 Mirror, top, adjustable
PN 4235961E 8.2-5
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-4 Mixing Module, Front View (See Table 8.2-4)

9
1
2
7
3
6
4
5961131C
8.2-6 PN 4235961E
PARTS LISTS
ILLUSTRATED PARTS 8
Table 8.2-4 Mixing Module, Front View (See Figure 8.2-4)

6915226 Mixing Chamber module
2 6232436 Fitting, Luer, 0.094 i.d., female
3 6232434 Ring, Luer lock, white (use with PN 6232435 and PN 6232436)
5 6857096 Pinch valve, single, miniature, round
6 6858748 Mixing chamber assembly
7 7000032 Pump, StabiLyse, 0.133 mL
8 6857019 Holder, mixing chamber
9 1016486 Isolator, feed-through fitting
PN 4235961E 8.2-7
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-5 Mixing Module, Right Side View (See Table 8.2-5)
1
2
8
3
5 4 5961132D
8.2-8 PN 4235961E
PARTS LISTS
ILLUSTRATED PARTS 8
Table 8.2-5 Mixing Module, Right Side View (See Figure 8.2-5)

1 1005699 Fitting, feed-through, 0.062 i.d., standard
2 2523221 Belt, mixing motor
3 6857900 Harness, Mixing Chamber module, P52 and J71
4 6232036 Fitting, 0.036, 10-32 standard
7 6857019 Holder, mixing chamber
8 7000474 Drive assembly, mixing chamber
9 6855763 Pinch valve, double acting, I-beam, removable, standard
PN 4235961E 8.2-9
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-6 Mixing Module, Left Side View (See Table 8.2-6)
5961133C
8.2-10 PN 4235961E
PARTS LISTS
ILLUSTRATED PARTS 8
Table 8.2-6 Mixing Module, Left Side View (See Figure 8.2-6)

1 7000487 Motor, mixing chamber, 24 Vdc
PN 4235961E 8.2-11
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-7 BSV Module with BSV that Uses Air Cylinders, Front View (See Table 8.2-7)
14
4
13
12
9
7 6 5
11
10
8 5961134E
8.2-12 PN 4235961E
PARTS LISTS
ILLUSTRATED PARTS 8
Table 8.2-7 BSV Module with BSV that Uses Air Cylinders, Front View (See Figure 8.2-7)

1 7000035 Tank, sheath
2 7000034 Pump, calibrated, air, 0.064 mL
3 6857096 Pinch valve, single, miniature, round (PV14 and PV15)
4 6859004 Cylinder, air, BSV front and center (CL1 and CL2)
5 2851460 Shoulder washer
6 1016788 Insulator (for BSV that uses air cylinders)
7 1017361 Shaft, BSV, coated (for BSV that uses air cylinders)
6859155 Housing, BSV panel (for BSV that uses air cylinders)
8 6806708 Sample (start) bar, Secondary mode
9 7000082 Rinse block
10 2827087 Washer, flat, vinyl (for rinse block)
11 2851915 Screw, shoulder, rinse block, securing
12 1020527 Rinse block arm, secondary
13 6855617 Knob, BSV (for BSV that uses air cylinders)
14 6707096 BSV, self cleaning, 3 sections (pads)
PN 4235961E 8.2-13
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-8 BSV Module with BSV that Uses an Actuator, Front View (See Table 8.2-8)
1 2 3 4
23
5
22
21
20
19 6
18
7
Configuration A
15 13 8
17 16
14
12
11
Configuration B
5961293E 9
10
8.2-14 PN 4235961E
PARTS LISTS
ILLUSTRATED PARTS 8
Table 8.2-8 BSV Module with BSV that Uses an Actuator, Front View (See Figure 8.2-8)

6915200 BSV module
1 7000078 Pump, Primary-mode aspiration, 0.185 mL
2 7000138 Pump, Secondary-mode aspiration, 0.125 mL
3 6857096 Pinch valve, single, miniature, round
4 7000035 Tank, sheath
5 7000034 Pump, calibrated, air, 0.064 mL
6 6232075 Solenoid, 3-way, 50 psi, 24 Vdc
7 6232277 Fitting, 0.093 i.d.
2523062 O-ring, 0.187 i.d. x 0.050 w
8 6232921 Actuator, valve, double acting, dual concentric shafts, and BSV cylinder knob and bracket
(configuration A actuator)
6232994 Actuator, valve, double acting, dual concentric shafts, and BSV cylinder knob and bracket
(configuration B actuator)
9 2527871 Bushings, centering (use with BSV actuator PN 6232994)
10 2527869 Bushing, shaft (use with BSV actuator PN 6232994)
11 2527868 Knob (use with BSV actuator PN 6232994)
12 2527870 Bracket, left section (pad) segmenting (use with BSV actuator PN 6232994)
13 1024398 Bushing (use with BSV actuator PN 6232921)
14 6806708 Sample (start) bar, Secondary mode
18 6707096 BSV, self cleaning, 3 sections (pads)
19 7000074 Blood detectors, matched set
22 6855763 Valve, pinch, double acting, I-beam, removable, standard
23 6859138 Foam trap, 3-port (FMT4)
PN 4235961E 8.2-15
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-9 BSV Module, Rear View (See Table 8.2-9) Figure Reference
1 A Ball and Slide Assembly,

Figure 8.2-10
4
5961135D
8.2-16 PN 4235961E
PARTS LISTS
ILLUSTRATED PARTS 8
Table 8.2-9 BSV Module, Rear View (See Figure 8.2-9)

1 6857904 Harness, BSV module, P51, J60, J75
2 7000037 Peltier module
3 7000087 Peltier Controller card
4 7000104 Fan and toroid, Peltier cell
PN 4235961E 8.2-17
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-10 BSV Module, Ball and Slide Assembly (See Table 8.2-10)
1 2
3
25
24 4
6
23
7
10
22
11
16
12
13
17
14
15
4
18
13
14
15
19
20
21
7535020A
8.2-18 PN 4235961E
PARTS LISTS
ILLUSTRATED PARTS 8
Table 8.2-10 BSV Module, Ball and Slide Assembly (See Figure 8.2-10)

1 6857953 Bracket, cylinder mounting (CL3)
2 2851918 Setscrew, 6-32, rinse block, adjustment
3 2806090 Screw, pan head, #6-32 x 0.50 (for CL3)
4 2827134 Washer, flat, #6, 0.56 i.d. x 0.375 o.d. x 0.046 thick (for CL3 bracket)
5 2826035 Washer, split lock, #6, 0.14 i.d. x 0.25 o.d. x 0.031 thick (for CL3 bracket)
6 2822030 Nut, hex, #6-32 unc x 0.312AF x 0.114 thick (for CL3 bracket)
7 2804083 Screw, hex, #4-40 x0.25 (for ball slide)
8 1020539 Retainer, tube (inner ball-slide stop)
9 1020521 Stop, ball slide
10 1020529 Retainer, tube (outer ball-slide stop)
11 2804074 Screw, machine, #4-40 x 0.31, flat82-HD (for ball-slide stop)
12 1020163 Retainer, tube (lower ball slide)
13 2827133 Washer, flat, #4 (for lower tube retainer)
14 2826002 Washer, split lock #4 (for lower tube retainer)
15 2804005 Screw, pan head, #4-40 x 0.25 (for lower tube retainer, rinse block arm)
16 2804011 Screw, machine, #4-40 x 0.25, flat82-HD (for rinse block arm)
17 1020525 Spacer, rinse block support
18 1020527 Rinse block arm, secondary
22 6232357 Cylinder, air pot, backwash arm (CL3)
23 6858031 Ball slide extension
24 2515004 Spring, fxt, 0.310 d (ball-slide return)
25 2823550 Bearing, ball slide
PN 4235961E 8.2-19
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-11 Pump Module, Old Configuration (See Table 8.2-11)
12
11
1 1
10
9
3
8
5961136D
6 5 4
8.2-20 PN 4235961E
PARTS LISTS
ILLUSTRATED PARTS 8
Table 8.2-11 Pump Module, Old Configuration (See Figure 8.2-11)

3 6858077 Tubing, special, 7 in., 0.023 i.d.
4 7000063 Pumps, matched set, Erythrolyse II reagent
5 7000138 Pump, Secondary-mode aspiration, 0.125 mL
6 7000078 Pump, Primary-mode aspiration, 0.185 mL
10 6854848 Chamber, isolator (VC4 with screen)
11 6857905 Harness, Pump module, P53
PN 4235961E 8.2-21
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-12 Pump Module, New Configuration (See Table 8.2-11)
10
1 1
7
3
6
4 5961294D
8.2-22 PN 4235961E
PARTS LISTS
ILLUSTRATED PARTS 8
Table 8.2-12 Pump Module, New Configuration (See Figure 8.2-12)

6915224 Pump module
3 6858077 Tubing, special, 7 in., 0.023 i.d.
4 7000063 Pumps, matched set, Erythrolyse II reagent
9 6857905 Harness, Pump module, P53
PN 4235961E 8.2-23
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-13 Rotary Cap-Pierce Module (See Table 8.2-13) Figure Reference
23 A Figure 8.2-14
1
2
22
21
20
6,7,8,
9,10
19
11
18
12
17
16
15 Configuration A
13
14
5961296E Configuration B
8.2-24 PN 4235961E
PARTS LISTS
ILLUSTRATED PARTS 8
Table 8.2-13 Rotary Cap-Pierce Module (See Figure 8.2-13)

6915187 Rotary Cap-Pierce module
1 6705746 RCP Junction II card
2 2830029 Grommet, black trim
3 Reference Bracket, cap piercer
4 7000042 Laser bar-code reader
5 6857949 Harness, opto (includes all sensors)
6 1020569 Screw, special
7 1020588 Lock, needle
8 1020596 Clip, needle retainer
9 2851828 Washer, spring (two needed)
10 6857991 Needle slide with opto sensor actuator
11 1020229 Bracket, dual opto, needle position
12 6232461 Cylinder, air, needle pierce (CL5)
13 6232999 Cylinder, air, with magnet
14 6805584 Sensor, Hall effect (for Rotary Cap-Pierce module only)
6232436 Fitting, Luer, 0.094 i.d., female
16 1020047 Collar, bellows
17 1020594 Needle slide
18 1020232 Bellows, needle
1020644 Slide (clip), bellows
7000056 Needle, FRU
7000088 Needle assembly, customer replaceable (QD4)
7000363 Needle assembly, customer replaceable (needle, bellows, collar, slide, and Luer fittings), FRU
19 6858111 Tray, tube catch, removable
20 6232376 Solenoid, 5-way
21 7000057 Motor, carousel (order four each 2523615 elastic shock mounts)
22 1019604 Bracket, magnetic switch
2523616 Latch, magnet
23 Reference Panel, carousel, auto-pierce
PN 4235961E 8.2-25
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-14 Rotary Cap-Pierce Module, Carousel Assembly (See Table 8.2-14)
25 6
1
24 3
2
23
4
5
22
21
20
19 7
18
8
17
16
9
15
10
14 11
13
12 5961257D
8.2-26 PN 4235961E
PARTS LISTS
ILLUSTRATED PARTS 8
Table 8.2-14 Rotary Cap-Pierce Module, Carousel Assembly (See Figure 8.2-14)

1 6858040 Carrier, tube holder (setscrews PN 2807080)
2 1020950 Cradle guide
3 2851768 Pin, pivot, tube available sensor
4 6857787 Lever, tube available sensor
5 1020694 Spring, tube available sensor
6 2838063 Holder, panel, grommet for LED
7 1020044 Bracket, code-wheel sensor
8 6857987 Bracket, carousel, black
9 1020054 Code wheel
10 1020390 Tube ejector (spacer two each, PN 2843056)
11 1020046 Retainer, bellows
12 1014581 Spring, holder
13 2515017 Spring, compression, cap-piercer, tube retainer
14 1020230 Saddle guide (hinge block PN 1014599, pin PN 2831012)
15 2831012 Pin, dowel (saddle guide)
16 1014599 Hinge block (saddle guide)
17 1014725 Spacer, tube clamp to air cylinder
18 1020576 Guide, lower, tube clamp
19 6859030 Bracket, tube clamp with bumper
20 1020577 Guide, upper, tube clamp
21 3213199 Bumper, rubber, tube clamp
22 1020571 Shaft, stub, threaded
23 2523199 Coupling, flex shaft
24 6858029 Nut plate (fits on motor vial guide side of carousel)
25 1020745 Vial guide, molded plastic, covers half of carousel shaft
PN 4235961E 8.2-27
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-15 Autoloader Module, Front View (See Table 8.2-15) Figure Reference
1,2 3 4 1,2 A Rocker bed hardware and

sensors, Figure 8.2-17
B Needle assembly and
5 5 sensors, Figure 8.2-18
7 7
8
9
22 10
11
21
12 12
20 19 18 B 17 16 15 14 13
5961091D
8.2-28 PN 4235961E
PARTS LISTS
ILLUSTRATED PARTS 8
Table 8.2-15 Autoloader Module, Front View (See Figure 8.2-15)

7000472 Autoloader module
1 1021600 Wheel, cassette shelf
2 1021601 Roller, cassette shelf
3 1021412 Actuator sensor, cone sensor
4 2851722 Tip, tube ram (use with Tube-ram insert [button] PN 1022607)
5 1021569 Spring, cassette shelf
6 1021311 Actuator body, cone sensor
7 1021393 Plate, motor mount
8 6858381 Encoder disk
9 1021599 Lock wheel, rocker bed
10 6858507 Sensor and harness, 15 in. (bed position sensor)
11 7000651 Bed lock assembly
12 6858505 Motor, cassette, lift
13 2523683 Rod bearing, right
14 1021293 Pivot-link drive
15 2523684 Rod bearing, left
16 6858474 Hub, pivot, rocker motor
17 2804083 Screw, rocker motor hub
18 6858506 Index motor, cassette
19 1021292 Hub, index motor
20 2851227 Screw, index hub shoulder
21 6858501 Interlock door switch
22 6858383 Link drive
PN 4235961E 8.2-29
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-16 Autoloader, Rear View (See Table 8.2-16)
7 1
4 5961092E
8.2-30 PN 4235961E
PARTS LISTS
ILLUSTRATED PARTS 8
Table 8.2-16 Autoloader, Rear View (See Figure 8.2-16)

1 6858499 Tube-ram sensor and harness
3 6705331 Autoloader Interface card (non CE)
6706469 Autoloader Interface card (CE)
4 6858413 Slide, rack, ball
5 6028385 Cable, ribbon, flat, Autoloader
6 6232597 Cylinder, tube ram
7 6232568 Solenoid manifold, SMC, dual bank
PN 4235961E 8.2-31
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-17 Autoloader, Rocker Bed Hardware and Sensors (See Table 8.2-17)
10
1
2
4
7 2
6 5 4 3 5961069D
8.2-32 PN 4235961E
PARTS LISTS
ILLUSTRATED PARTS 8
Table 8.2-17 Autoloader Module, Rocker Bed Sensors and Hardware (See Figure 8.2-17)

7000639 Rocker bed assembly
1 1021296 Pin, pivot
2 6858739 Sensor and harness, cassette 0 and 3
3 1021634 Guide, cassette platform
4 6858741 Sensor and harness, cassette 1 and 2
5 6858609 Load (right) elevator down sensor and harness
6 6858496 Tube available sensor and harness, 7 in.
7 1022161 Guide, cassette bed
8 6858494 Full cassette index rotation sensor and harness, 13 in.
9 6858498 Unload (left) elevator down sensor and harness
10 2851892 Washer, spring
PN 4235961E 8.2-33
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-18 Needle Assembly and Sensors (See Table 8.2-18)
1 2
10
9 8
Configuration A
7 5
6
1 2
10
8
Configuration B 9
7
11
5
12 5961297E
8.2-34 PN 4235961E
PARTS LISTS
ILLUSTRATED PARTS 8
Table 8.2-18 Autoloader Module Needle Assembly and Sensors (See Figure 8.2-18)

1 6858476 Dead plate
2 7000447 Needle cartridge
3 6859268 Guide, needle slide
4 1021655 Bellows, needle
5 7000201 Bracket, needle cartridge driver
6 6232620 Cylinder, needle
7 1022462 Tube-forward sensor arm
8 6232601 Cylinder, tube return
9 6858507 Sensor and harness, 15 in. (tube forward, needle forward, and needle home sensors on
Configuration A, tube forward sensor on Configuration B )
10 1022099 Retainer, bellows
11 6806247 Sensor, Hall effect (for Autoloader module only)
12 6232999 Cylinder, air, with magnet
PN 4235961E 8.2-35
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-19 Triple Transducer Module (See Table 8.2-19) Figure Reference
A Flow cell and laser assembly,
2
Figure 8.2-20
1
4
7
3 5
5961259D
8.2-36 PN 4235961E
PARTS LISTS
ILLUSTRATED PARTS 8
Table 8.2-19 Triple Transducer Module (See Figure 8.2-19)

7000646 TTM assembly, complete
1 6858361 Shield, flow cell (cover)
2 1020249 Shield, magnetic (laser cover)
3 2851566 Screw, tamperproof
4 5704031 Vacuum tube, RF triode, 12AU7A (new style)
5 6859777 RF Detector Preamp #6 assembly
6 7000038 Base and laser mount
7 7000040 LS Preamp 3 module (for instruments without the Retic option)
6859066 LS Preamp 5 module
PN 4235961E 8.2-37
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-20 Flow Cell and Laser Assembly (See Table 8.2-20)
18
1
20
2
19
9 3
4
6
5
16
17 18
16
7
10
9
10
9
14
BOTTOM VIEW
16
8 11
15
13
12
5961260D
REAR VIEW
8.2-38 PN 4235961E
PARTS LISTS
ILLUSTRATED PARTS 8
Table 8.2-20 Flow-Cell and Laser Assembly (See Figure 8.2-20)

1 6706039 Flow cell, coated and tested
2 6706013 Sensor, LS, 3-element (Rev. B), for retic ready units (use with LS Preamp 5 module)
Sensor, LS (Rev. E), for instruments without the retic option (use with LS Preamp 3 module)
3 2808131 Screw, 2.25 l, #8 x 32, pan-hd, xrec
4 2826048 Washer, flat, #2, 0.094 i.d. x 0.188 o.d. x 0.028 thick
5 2827135 Washer, flat, #8, 0.187 i.d. x 0.438 o.d. x 0.063 thick
6 7000551 Lens block
7 2851567 Screw, threaded, 1.57 l, #10 x 56, Y- and Z-axes alignment
8 2827160 Washer, flat, #8, 0.188 i.d. x 0.375 o.d. x 0.045 thick, X/Y/Z alignment shaft
9 2515158 Spring, TTM X-, Y-, Z-alignment shafts
10 2851569 Nut, square, #10, used on Y- and Z-axes
11 6856153 Plates, TTM, horizontal and vertical adjustment
12 2807038 Setscrew, 10-32 x 0.25 long, hex (flow-cell lock down)
13 1017940 Shaft, TTM, X-axis (horizontal) adjustment
14 2851568 Nut, hex, #10, used on X-axis
15 2852276 Screw, 0.62 long, #8 x 32, hex-head, ehex
16 2852270 Washer, lock, spring, 0.164 i.d. x 0.343 o.d. x 0.013 thick
17 2851614 Screw, 0.62 long, #8 x 32, TTM lock down
18 2808110 Screw, 0.50 long, #8 x 32, hsc-head, hex
19 2806009 Screw, 0.25 long, #6 x 32, pan-head, xrec (flow-cell mount)
20 1019356 Plate, TTM, flow-cell mounting (Rev. C)
PN 4235961E 8.2-39
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-21 Main Diluter Module (See Table 8.2-21)

7 20 21
4 1
19
18 2
17
16
15
14 3
13
12 4
11
10
5961270D 9 8 7 6 5
8.2-40 PN 4235961E
PARTS LISTS
ILLUSTRATED PARTS 8
Table 8.2-21 Main Diluter Module (See Figure 8.2-21)

1 6705768 Fluid Detector/Ram Pressure card
2 7000020 Pneumatic Monitor card
5 7000607 Optical reagent sensor and housing (lytic reagent and PAK)
6 7000606 Optical reagent sensor and housing (diluent and cleaning agent)
8 6856319 Pinch valve, triple
9 6806053 Pump, backwash, 3 mL (PM8)
10 7000071 Regulator, sample/sheath pressure (RG3, RG4)
11 6854671 Manifold, 30 psi (MF14)
12 7000068 Water trap, vacuum (FL1)
13 7000067 Water trap, compressor (FL2)
14 7000070 Solenoid, condensate drain (LV43)
15 7000069 Regulator, 30 psi (RG2)
16 7000055 Choke, sheath, #2, reg (CK30)
17 7000064 Pump, dispense, 5 mL (PM10, cleaning agent) (PN 6806058)
18 6912099 Dispenser, WBC, 6 mL
19 7000145 Regulator, low vacuum (RG1) (6232628-0)
21 6905043 Dispenser, RBC, 10 mL
PN 4235961E 8.2-41
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-22 Electronic Power Supply, Top View (See Table 8.2-22)
1
DANGER
12 10
12
3
3
J119
J114
J116
J108
15
3
10
1
1
1
J105
J115
6
3
6
3
J113
J112
13
1
J107
1
1
4
4
1
1
2
120V
4
7
6
5
5961249D
8.2-42 PN 4235961E
PARTS LISTS
ILLUSTRATED PARTS 8
Table 8.2-22 Electronic Power Supply, Top View (See Figure 8.2-22)

7000004 Electronic Power Supply module, 100 Vac (non-CE)
7000005 Electronic Power supply module, 120 Vac (non-CE)
7000006 Electronic Power Supply module, 220/240 Vac (non-CE)
7000462 Electronic Power Supply module, 100 Vac (CE)
7000463 Electronic Power supply module, 120 Vac (CE)
7000464 Electronic Power Supply module, 220/240 Vac (CE)
1 7000066 Fan, Electronic Power Supply module, toroid, 24 Vdc
2 7000043 Aperture Voltage/Power Fail Detect card, 100 Vac
7000044 Aperture Voltage/Power Fail Detect card, 120 Vac
7000045 Aperture Voltage/Power Fail Detect card, 220 Vac
3 7000046 Power supply, RF
4 7000003 Power supply, +5 Vdc, 12 A ±15 Vdc (PS3)
5 7000001 Power supply, +5 Vdc, 5 A (PS7, 5.6 V supply)
6 7000000 Power supply, +24 Vdc (PS4)
7 7000002 Power supply, +12 Vdc, 6.3 A (PS6)
PN 4235961E 8.2-43
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-23 Pneumatic Power Supply, Left-Side View (See Table 8.2-23)
1
5961250D
8.2-44 PN 4235961E
PARTS LISTS
ILLUSTRATED PARTS 8
Table 8.2-23 Pneumatic Power Supply, Left-Side View (See Figure 8.2-23)

7000122 Pneumatic Power Supply module, 100 Vac
7000061 Pneumatic Power Supply module, 100/120 Vac
7000062 Pneumatic Power Supply module, 220/240 Vac
1 6232657 Pump, vacuum/pressure, 100/115 Vac
6232658 Pump, vacuum/pressure, 220/230 Vac
2 2602022 Fan, 230 Vac (use with Pneumatic Power Supply module, 220/240 Vac)
2602023 Fan, 115 Vac (use with Pneumatic Power Supply module, 100/110 Vac)
3 6208005 Regulator, 60 psi
4 2523590 Mount, compressor shock, 6 lb
PN 4235961E 8.2-45
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-24 Analyzer Module, Card Cage Components (See Table 8.2-24)
1 2 3 4 5 6
W
H
T
LYSE A
TIMMING P
E
R
T
WADJ
WIA
7
CONTROL RIA
LYSE TRIG VOLUUM
RADJ
V
MIX
H
C
GRD
L
S
R
GND E
GND
D
A
P
L PROBE E
R
T
5961251D
8.2-46 PN 4235961E
PARTS LISTS
ILLUSTRATED PARTS 8
Table 8.2-24 Analyzer Module, Card Cage Components (See Figure 8.2-24)

1 7000026 376 CPU card
2 7000024 DILUTER INTERFACE card
3 7000023 I/O card
4 7000028 VCS PROCESSOR card
5 7000027 R/W/P PROC card
6 7000025 R/W Preamp card
7 6858109 Card cage, with backplanes
PN 4235961E 8.2-47
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-25 Wire Harness Components (See Table 8.2-25)

1
(J150)
18 2
(J82) (P1)
18 3
(J80) (P91)
4
15 (P79)
(J54)
15 5
(J51) (J32)
6
(J32)
7
(J69)
5
8
(J77)
(J106)
9
(P104)
2 17 16 15 15 14 13 12 11 10
5961275D (P4) (P5) (J26) (J53) (J52) (P113) (P78) (P109) (P73) (P66)
8.2-48 PN 4235961E
PARTS LISTS
ILLUSTRATED PARTS 8
Table 8.2-25 Wire Harness Components (See Figure 8.2-25)

1 2104208 Clamp, connector, circular CPC (J50)
2121687 Connector, circular, CPC, 57-pin (J50)
2 2121442 Connector (P1, P4 for Peltier module)
3 2121280 Connector (P91)
4 2104318 Contact, socket, 24-20 AWG, square post, SC52 (P79)
2121454 Connector (P79)
5 2104357 Connector, two conductor, universal Mat-N-LK (J32 for instruments with optical reagent sensors,
and J77)
6 2104347 Connector, 15-conductor, universal Mat-N-LK (J32 for instruments with float reagent sensors)
2121425 Fifteen-position assembly module (J32 for instruments with float reagent sensors)
2121436 Adapter for cap housing (J32, for instruments with float reagent sensors)
7 2104351 Connector, nine conductor, universal Mat-N-LK, female (J69 for TTM)
2121426 Nine-position assembly module (J69 for TTM)
2121436 Adapter (J69 for TTM)
8 2104361 Connector, 3-conductor (J106 for TTM)
9 2104318 Contact, socket, 24-20 AWG, square post, SC52 (P104 for RF Detector Preamp card))
2121415 Connector, 4-position (P104 for RF Detector Preamp card)
10 2104318 Contact, socket, 24-20 AWG, square post, SC52 (P66 for LS Preamp module)
2121328 Connector, 3-position (P66 for LS Preamp module)
11 6027923 Cable, RF Detector Preamp card to backplane (P73)
12 6705938 Harness, Retic Interface, and card (P109)
13 6028274 Cable, LS Preamp module to backplane (P78 to P49)
14 6028480 Cable, LS LS offset monitor (P113 to P7 [A13 and B38])
15 2104334 Clamp, connector, circular, CPC (J51, J52, J53, J54)
2121684 Connector, circular, CPC, 28-pin (J51, J52, J53, J54)
16 2104258 Clamp, connector, circular, CPC (J26)
2121685 Connector, circular, CPC, 8-pin (J26)
17 2104360 Connector, 3-position, male (P5 for Peltier module)
18 2104258 Clamp, connector, circular, CPC (J80, J82)
2104339 Connector, circular, CPC, 8-pin (J80, J82)
PN 4235961E 8.2-49
PARTS LISTS
ILLUSTRATED PARTS
Figure 8.2-26 Electrical Connectors Table 8.2-26 Electrical Connectors
1 2 Item Part Number Description

1 2104189 Contact, pin, 24-20
2 2104190 Contact, socket
3 2104348 Pin, connector, universal Mat-N-LK, 24-18 AWG,
3 4 brass
4 2104349 Socket T, connector, universal Mat-N-LK,
24-18 AWG, brass
5 2104318 Contact, socket, 24-20 AWG, square post
5 6 2121421 Contact, socket, 26-22 AWG, square post
6 2113052 Terminal, solderless, #5 spring spade,
22-16 AWG
7 2104418 Holder, socket, crimp terminal, 24-18 AWG,
Molex
7 8
8 2121420 Terminal, solderless receptacle, 90 degree,
22-18 AWG
9 2113022 #6 ring, 16-14 AWG, insulated
2113018 #6 ring, 22-16 AWG, insulated
2113010 #8 ring, 22-16 AWG, insulated
9 10
10 2113053 Terminal receptacle, 22-18 AWG
2121050 Terminal receptacle, 22-18 AWG
2113045 Terminal receptacle, 22-18 AWG
5961276D
8.2-50 PN 4235961E
CONTENTS
9
A QUICK REFERENCE INFORMATION, A.1-1
A.1 TOLERANCES AND LIMITS, A.1-1

Calibration Tolerances, A.1-1
Electronic Noise Limits, A.1-2
Performance Specifications, A.1-2
Background Counts, A.1-2
Carryover Limits, A.1-2
Reproducibility/Precision Limits, A.1-3
Secondary Mode- to- Primary Mode Comparison, A.1-4
Pneumatic Tolerances, A.1-5
Power Input Specifications and Typical Consumption, A.1-5
Input Power, A.1-5
Input Line Interference, A.1-5
Power Consumption, A.1-6
Timing, A.1-7
VCS Optimization Limits, A.1-8
Voltages, A.1-9
System, A.1-9
Volumes, A.1-10
Aspiration Pump, Primary Mode, A.1-10
Diluent Dispensers, A.1-10
CBC Lytic Reagent Pumps’ Volume Tolerance, A.1-10
A.2 CIRCUIT CARDS - JUMPERS AND TEST POINTS, A.2-1

376 CPU Card, A.2-1
Autoloader Interface Card, A.2-2
Connectors, A.2-2
DILUTER INTERFACE Card, A.2-3
Test Point, A.2-3
Fluid Detector/Ram Pressure Card, A.2-4
Connectors, A.2-4
LEDs, A.2-4
I/O Card, A.2-5
Peltier Controller Card, A.2-6
Connectors and LEDs, A.2-6
Pneumatic Monitor Card, A.2-7
Connector, A.2-7
Test Points, A.2-8
RAM Timer Card, A.2-9
Rocker Bed Interface Card, A.2-10
Connectors, A.2-10
R/W/P PROC Card, A.2-11
Test Points, A.2-11
PN 4235961E 9-i
CONTENTS

Blood/Bubble Detector Adjustments and Test Points - Sample Handler I
Card, A.2-12
Connectors - Sample Handler I Card, A.2-12
Jumper Settings - Sample Handler I Card, A.2-13
LEDs - Sample Handler I Card, A.2-13
Blood/Bubble Detector Adjustments and Test Points- Sample Handler II
Card, A.2-14
Connectors - Sample Handler II Card, A.2-14
Jumper Settings- Sample Handler II Card, A.2-14
LEDs- Sample Handler II Card, A.2-15
VCS PROCESSOR Card, A.2-16
Test Points, A.2-16
A.3 POWER SUPPLIES - SWITCH SETTINGS; FUSE RATINGS; AND

CONNECTIONS, JUMPERS, AND TEST POINTS, A.3-1
Power Switch Settings, A.3-1
Fuse Ratings and Functions, A.3-1
Electronic Power Supply, A.3-2
Test Points and Adjustments, A.3-2
Laser Power Supply, A.3-4
Pneumatic Power Supply, A.3-4
A.4 SOFTWARE SETTINGS, A.4-1

Bar-Code Reader Decoder Card, A.4-1
A.5 DILUTER COMPONENT LOCATIONS AND FUNCTIONS, A.5-1
A.6 SOFTWARE MENU TREES, A.6-1
B DMS DATABASE FIELDS, B.1-1
B.1 FIELDS CONTAINED IN THE MAXM ANALYZER DATABASE, B.1-1

About the List, B.1-1
MAXM Analyzer 8D Database Fields, B.1-1
ILLUSTRATIONS
A.1-1 Diluent and Lyse Timing Cycle, A.1-7
A.2-1 376 CPU Card Jumper Locations, A.2-1

A.2-2 Autoloader Interface Component Locations, A.2-2
A.2-3 DILUTER INTERFACE Card Component Locations, A.2-3
A.2-4 Fluid Detector/Ram Pressure Card Component Locations, A.2-4
A.2-5 I/O Card Jumper Locations, A.2-5
A.2-6 Peltier Controller Card Connector and LED Locations, A.2-6
A.2-7 Pneumatic Monitor Card Component Locations, A.2-7
9-ii PN 4235961E
CONTENTS
9
A.2-8 RAM Timer Card Jumper Locations, A.2-9
A.2-9 Rocker Bed Interface Card Connector Locations, A.2-10
A.2-10 R/W/P PROC Card Component Locations, A.2-11
A.2-11 Sample Handler I Card Component Locations, A.2-12
A.2-12 Sample Handler II Card Component Locations, A.2-14
A.2-13 VCS PROCESSOR Card Component Locations, A.2-16
A.5-1 BSV Module, Old Configuration, Front View, A.5-7

A.5-2 BSV Module, New Configuration, Front View, A.5-7
A.5-3 BSV Module, Rear View, A.5-8
A.5-4 CBC Module, Front View, A.5-8
A.5-5 CBC Module, Rear View, A.5-9
A.5-6 Main Diluter Module, Front View, A.5-9
A.5-7 Main Diluter Module, Right-Side View, A.5-10
A.5-8 Mixing Module, Front View, A.5-10
A.5-9 Mixing Module, Rear View, A.5-11
A.5-10 Pump Module, Old Configuration, A.5-11
A.5-11 Pump Module, New Configuration, A.5-12
A.6-1 MAXM Analyzer with Rotary Cap-Pierce Module Software Menu Tree, Revision
8D and Higher, A.6-1
A.6-2 MAXM Analyzer with Autoloader Module Software Menu Tree, Revision 8D and
Higher, A.6-2
A.6-3 Service Disk Software Utility Menu Tree, A.6-3
TABLES
A.1-1 Acceptable Cal Factor Ranges, A.1-1
A.1-2 Acceptable CV Limits for Initial Adjustments to 5C Cell Control, A.1-1
A.1-3 CBC Latex Calibration, A.1-1
A.1-4 RMS Noise Checks - CBC, A.1-2
A.1-5 Primary/Secondary-Mode Background Counts, A.1-2
A.1-6 Primary/Secondary-Mode Carryover Limits, A.1-2
A.1-7 Retic Mode-to-Mode and Within-Mode Carryover Limits*, A.1-2
A.1-8 Acceptable Blood Parameter Limits for Reproducibility Specimen, A.1-3
A.1-9 Acceptable Manual Diff Limits for Reproducibility Specimen, A.1-3
A.1-10 CBC and Diff Reproducibility Limits, A.1-4
A.1-11 Retics Reproducibility Limits, A.1-4
A.1-12 Secondary Mode-to-Primary Mode Comparison Limits, A.1-4
A.1-13 Pressure and Vacuum Tolerances, A.1-5
A.1-14 Input Line Interference Limits, A.1-5
A.1-15 Typical Power Consumption at 120 Vac, A.1-6
A.1-16 Diluent and Lyse Timing Acceptable Choke Combinations, A.1-7
A.1-17 LATRON™ Primer Background Limits, A.1-8
A.1-18 LATRON™ Control Calibration and Verification Limits, A.1-8
A.1-19 LS Offset Voltage/Laser On Current Checks, A.1-8
A.1-20 Noise Checks - Diff and Retic, A.1-8
A.1-21 Hgb Blank Voltage, A.1-9
A.1-22 Blood/Bubble Detectors Tolerances, A.1-9
A.1-23 System Voltage Ranges, A.1-9
PN 4235961E 9-iii
CONTENTS
A.1-24 Aspiration Pump Volume Tolerances, A.1-10

A.1-25 Diluent Dispenser Volume Specifications and Tolerances, A.1-10
A.2-1 376 CPU Card Jumper Settings (See Figure A.2-1), A.2-1
A.2-2 Autoloader Interface Card Connectors (See Figure A.2-2), A.2-2
A.2-3 Fluid Detector/Ram Pressure Card Connectors (See Figure A.2-4), A.2-4
A.2-4 Fluid Detector/Ram Pressure Card LED Positions (See Figure A.2-4), A.2-4
A.2-5 Peltier Controller Card Connectors and LEDs (See Figure A.2-6), A.2-6
A.2-6 Pneumatic Monitor Card Pneumatic Reading to Vdc Conversion (See
Figure A.2-7), A.2-8
A.2-7 Rocker Bed Interface Card Connectors (See Figure A.2-9), A.2-10
A.2-8 R/W/P PROC Card Jumper Settings (See Figure A.2-10), A.2-11
A.2-9 R/W/P PROC Card Test Points (See Figure A.2-10), A.2-11
A.2-10 Sample Handler I Card Connectors (See Figure A.2-11), A.2-12
A.2-11 Sample Handler I Card Jumper Settings (See Figure A.2-11), A.2-13
A.2-12 Sample Handler I Card LED Functions (See Figure A.2-11), A.2-13
A.2-13 Sample Handler II Card Connectors (See Figure A.2-12), A.2-14
A.2-14 Sample Handler II Card Jumper Settings (See Figure A.2-12), A.2-14
A.2-15 Sample Handler II Card LED Functions (See Figure A.2-12), A.2-15
A.2-16 VCS PROCESSOR Card Test Points (See Figure A.2-13), A.2-16
A.3-1 Input Power Switch Settings, A.3-1

A.3-2 Fuse Ratings and Functions, A.3-1
A.3-3 Electronic Power Supply Terminal Card Jumpers and Connections, A.3-2
A.3-4 Electronic Power Supply Test Points and Adjustments, A.3-2
A.3-5 Laser Power Supply Buck-Boost Transformer Jumpers and Connections, A.3-4
A.3-6 Pneumatic Power Supply Buck-Boost Transformer Terminal Card Jumpers and
Connections, A.3-4
A.4-1 Bar-Code Reader Decoder Card Code Types Default Settings, A.4-1
A.4-2 Bar-Code Reader Decoder Card Communications Default Settings, A.4-1
A.4-3 Bar-Code Reader Decoder Card Operations Default Settings, A.4-2
A.4-4 Bar-Code Reader Decoder Card User Outputs Default Settings, A.4-2
A.5-1 Diluter Component Location References and Functions, A.5-1
9-iv PN 4235961E
AQUICK REFERENCE INFORMATION A
A.1 TOLERANCES AND LIMITS
Calibration Tolerances
Table A.1-1 Acceptable Cal Factor Ranges
Primary-Mode Cal Factor

Minimum to Maximum Value
Secondary-Mode Cal Factor
Parameter Service Limits Software Limits Minimum to Maximum Value
WBC 0.965 to 1.295 0.960 to 1.300 0.500 to 2.000
RBC 0.965 to 1.295 0.960 to 1.300 0.500 to 2.000
Hgb 1.005 to 1.381 1.000 to 1.386 0.500 to 2.000
MCV 0.805 to 1.055 0.800 to 1.060 0.500 to 2.000
Plt 0.930 to 1.345 0.925 to 1.350 0.500 to 2.000
MPV 0.880 to 1.145 0.875 to 1.150 0.500 to 2.000
Table A.1-2 Acceptable CV Limits for Initial Adjustments to 5C Cell Control
Parameter CV (%)
WBC <2.0
RBC <1.5
Hgb <1.0
MCV <1.0
Plt <4.0
MPV <2.0
Table A.1-3 CBC Latex Calibration
Counts/Aperture Current Voltages Service Limits Performance Specifications

WBC count 3.0 to 30.0
RBC count 0.006 to 0.050
RBC mode Assay value ±1.5 fL
WBC mode Assay value ±1.5 fL
RBC aperture current 141.8 to 168.8 Vdc* 141.5 to 169.1 Vdc
WBC aperture current 100.9 to 129.3 Vdc* 100.6 to 129.6 Vdc
* Value obtained using System Test.
PN 4235961E A.1-1
QUICK REFERENCE INFORMATION
TOLERANCES AND LIMITS
Electronic Noise Limits

Table A.1-4 RMS Noise Checks - CBC
TP (R/W/P PROC card)* Specifications

RBC <18 mVac
WBC <75 mVac
PLT <59 mVac
* Read with a true RMS meter while the instrument is in the idle state.
For the RMS noise check specifications for Diff and Retic, see Table A.1-20, Noise Checks -
Diff and Retic.
Performance Specifications
Background Counts
Table A.1-5 Primary/Secondary-Mode Background Counts
Parameter Primary/Secondary Mode

WBC 0.4
RBC 0.04
Hgb 0.1
Plt 3.0
Diff <80/100
Carryover Limits
Table A.1-6 Primary/Secondary-Mode Carryover Limits
Parameter Primary/Secondary Mode

WBC 2%
RBC 1%
Hgb 2%
Plt 2%
Count 3%
Table A.1-7 Retic Mode-to-Mode and Within-Mode Carryover Limits*
Limits†
Rinse LLLL MMMM RRRR

First rinse N/A <394 N/A
Third rinse <215 N/A <265
* Ensure the blood sample used for this test has a diff cell count >6000/8192
† Numbers are in fields below the scatterplot in this format - LLLL/MMMM/RRRR.
A.1-2 PN 4235961E
TOLERANCES AND LIMITS A
Reproducibility/Precision Limits
ATTENTION: For reproducibility studies, use specimens with values that fall within the limits
specified in Tables A.1-8 and A.1-9.
Table A.1-8 Acceptable Blood Parameter Limits for Reproducibility Specimen
Parameter Low Limit High Limit Unit of Measure

WBC 3.6 9.9 x 103 cells/µL
RBC 3.9 (female) 5.0 (female) x 106 cells/µL
4.5 (male) 5.7 (male)
Hgb 12.1 (female) 15.1 (female) g/dL
13.8 (male) 17.2 (male)
Hct 36.1 (female) 44.3 (female) %
40.7 (male) 50.3 (male)
MCV 82.2 97.4 fL
MCH 27.6 33.3 pg
MCHC 33.0 34.8 g/dL
RDW 11.6 13.7 %
Plt 202.0 386.0 x 103/µl
MPV 7.8 11.0 fL
LY 20.0 55.0 %
MO 2.5 10.0 %
NE 37.0 73.0 %
EO 0.5 11.0 %
Table A.1-9 Acceptable Manual Diff Limits for Reproducibility Specimen
Parameter Low Limit High Limit Unit of Measure

Blasts 0 0.02 x 103 cells/µL
Atypical Lymphs 0 0.20 x 103 cells/µL
Bands 0 0.15 x 103 cells/µL
Metas 0 0.05 x 103 cells/µL
Promyelos 0 0.05 x 103 cells/µL
Eos 0 0.70 x 103 cells/µL
Basos 0 0.20 x 103 cells/µL
NRBC 0 2.00
Platelet Clumps None None
PN 4235961E A.1-3
Table A.1-10 CBC and Diff Reproducibility Limits
CBC 5-Part Diff
Parameter CV Parameter Maximum Range - Lowest to Highest

WBC 2.0% LY% <2.74
RBC 1.5% MO% <2.74
Hgb 1.0% NE% <3.04
MCV 1.05 EO% <1.44
RDW 2.0% BA% <1.34
Plt 4.0%
MPV 2.5%
Table A.1-11 Retics Reproducibility Limits
RET % CV %* 2 SD*
<1.00 <20.85 0.416
>1.00 <4.00 <15.41 0.416
>4.00 <15.00 <13.59 1.232
* Whichever is greater.
Secondary Mode- to- Primary Mode Comparison
Table A.1-12 Secondary Mode-to-Primary Mode Comparison Limits
Parameter Comparison Limits (whichever is greater)

WBC ±0.4 x 103 cells/µL or <5%
RBC ±0.20 x 106 cells/µL or <2%
Hgb ±0.3 g/dL or <2%
Plt ±20 x 103 cells/µL or <7.0%
LY% 4.04
MO% 4.04
NE% 4.24
EO% 2.04
BA% 2.04
A.1-4 PN 4235961E
Pneumatic Tolerances
Table A.1-13 Pressure and Vacuum Tolerances
Pressure* Acceptable Range* Vacuum* Acceptable Range*

60 psi 55 - 65 psi Hi Vac (22 in. Hg) 17 - 28 in. Hg
60 psi at peak load >38 psi Low Vac (6.0 in. Hg) 5.94 - 6.06 in. Hg
30 psi 26 - 34 psi
Sheath (6 psi) 5.8 - 6.2 psi
Diff (0.8 psi) 0.1 - 1.0 psi
*For equivalent voltage readings, see Table A.2-6.
Power Input Specifications and Typical Consumption
Input Power
The MAXM analyzer must have its input power strapped to match the following correct
input:
100 Vac ±10% 220 Vac ±10%

115 Vac ±10% 230 Vac ±10%
120 Vac ±10% 240 Vac ±10%
Input Line Interference

The instrument will operate as specified if the ac line interference remains within the limits
specified in Table A.1-14.
Table A.1-14 Input Line Interference Limits
Line dropout 27 ms
Line sag Withstands -20%, duration of 120 ms
Line surge Withstands +20%, duration of 50 ms
Line oscillation Withstands ±20%, 120 ms sag, 50 ms surge
Frequency variation Withstands ±2 Hz, 17 ms low, 16 ms high
Harmonic distortion Withstands 6%
Electrical fast transients Withstands 5 ns pulses in all coupling modes, all lines at <0.5 kV (tested
per IEC 801-5)
Surge immunity Withstands surges in different coupling modes, up to 2 kV (tested per
IEC 801-5)
PN 4235961E A.1-5
Power Consumption
Table A.1-15 Typical Power Consumption at 120 Vac
Operating Status Power at 120 Vac Power at 132 Vac

Standby mode (POWER switch off) 10 W 15 W
Power up 770 W 910 W
Instrument idle (Peltier off) 490 W 570 W
Instrument off (Peltier on) 550 W 625 W
Cycling (Primary mode) 570 W 660 W
Compressor off (POWER switch on) 360 W 410 W
A.1-6 PN 4235961E
Timing
Pressure setting for CBC lytic reagent and diluent delivery timing check: 30 psi ±0.1
Figure A.1-1 Diluent and Lyse Timing Cycle
PRESSURE
(PSI)
T T
2 3
T
4
T
0
T
1
0.88 ±0.10
TIME
LYTIC REAGENT (SECONDS)
0 2.00 ±0.12 0.62 ±0.12
DELIVERY
3.50 ±0.05
DILUENT DELIVERY
TIME TOLERANCE FUNCTION
T0 - T2 2.0 ±0.12 s INITIAL WBC DILUENT DISPENSE BEFORE LYTIC REAGENT IS ADDED.
T2 - T3 0.88 ±0.10 s LYTIC REAGENT ADDED.
T3 - T4 0.62 ±0.12 s FINAL WBC DILUENT DISPENSE AFTER LYTIC REAGENT IS ADDED.
T0 - T4 3.50 ±0.05 s TOTAL WBC DILUENT AND LYTIC REAGENT DISPENSE.

5961028D
Table A.1-16 Diluent and Lyse Timing Acceptable Choke Combinations
CBC Lytic Reagent Pumps*
WBC Diluent Dispenser* Pressure Line Reagent Line

0.010 i.d. (black) 0.010 i.d. (black) -
0.012 i.d. (blue) 0.012 i.d. (blue) -
0.016 i.d. (green) No choke -
Variable choke No choke or variable choke 4.75 in. lytic reagent restrictor or
7.5 in. lytic reagent restrictor
* Restrict the flow to increase timing, open the flow to decrease timing.
PN 4235961E A.1-7
VCS Optimization Limits

Table A.1-17 LATRON™ Primer Background Limits
Service Specifications Performance Specifications

≤100 ≤200
Table A.1-18 LATRON™ Control Calibration and Verification Limits
LATRON Diff and Retic Diff Retic

Control
Results DC (Volume) RF (Conductivity) LS LS Avg 5 LS LS Avg 5
Calibration Mean Assay ±0.5 Assay +0.0/-1.0 Assay ±4.0 Assay ±2.0 Assay ±8.0 Assay ±5.0
Limits* CV % <4.5% <6.0% <4.5% <4.5%
Mean-Mode ±0.5 ±0.6 ±1.5 ±3.0
Verification Mean Assay ±1.0 Assay +0.5/-1.5 Assay ±4.0 Assay ±2.0 Assay ±8.0 Assay ±5.0
Limits* CV % <5.0% <7.0% <5.0% <5.0%
Mean-Mode ±0.5 ±0.6 ±1.6 ±3.2
* Note: The limits you should use, calibration or verification, is based on why you are checking the results.
r If you are collecting this data following DC, RF, or LS gain adjustments; as part of VCS optimization; or as part of
instrument installation; compare the results to the tighter, calibration limits.
r If you are collecting this data for any other reason, compare the results to the verification limits. If you have trouble
meeting this specification, refer to Heading 7.4, DC, RF, OR LS LATEX CALIBRATION PROBLEMS.
Table A.1-19 LS Offset Voltage/Laser On Current Checks
Test Point Reference Diff Mode Specifications Retic Mode Specifications

LS Preamp 5 TP1-TP3/LS sensor <2.0 V/5 µA <1.1 V
Table A.1-20 Noise Checks - Diff and Retic
Diff Mode Specifications Retic Mode Specifications
RMS Noise TP Static Dynamic Static Dynamic

V <7.5 mV <12 mV <7.5 mV <12 mV
C <50 mV <50 mV <50 mV <50 mV
S <10 mV <12 mV <18 mV <20 mV
A.1-8 PN 4235961E
Voltages
Table A.1-21 Hgb Blank Voltage
Voltage Reading Service Limits Performance Specifications

Hgb blank 6.70 - 7.30 Vdc 6.65 - 7.35 Vdc
Sample Handler Cards
Table A.1-22 Blood/Bubble Detectors Tolerances
Voltage TP Adjustment Limits

Front Detector Cable Reading 5 (negative) and 23 (positive) R64 ±2 mV
Rear Detector Cable Reading 5 (negative) and 19 (positive) R77 ±2 mV
Front and Rear Recovery 19 and 23 Run Samples function ±5 mV
System
Table A.1-23 System Voltage Ranges
Performance
Voltage Service Limits* Specifications J120 TP Source Adjustment Fuse†
+5 Vdc 4.80 to 5.20 Vdc 4.75 to 5.25 Vdc 1 PS3 R10 F4
+5.6 Vdc 5.30 to 5.75 Vdc 5.32 to 5.88 Vdc 2 PS7 R7 F3
-15 Vdc -14.40 to 15.60 Vdc -14.25 to 15.75 Vdc 4 PS3 R31 F4
ac input N/A N/A N/A N/A N/A F1 and F2
+6.3 Vdc 6.05 to 6.55 Vdc 5.98 to 6.62 Vdc 8 RF Power Supply R1 F5
Ground N/A N/A 9 N/A N/A N/A
+240 Vdc 230 to 263 Vdc 228 to 265 Vdc N/A Aperture N/A N/A
Voltage/Power Fail
Detect card
+1350 Vdc 1196 to 1513 Vdc 1186 to 1523 Vdc N/A Laser Power Supply N/A F9
+300 Vdc** 288 to 312 Vdc 285 to 315 Vdc N/A RF Power Supply R2 F8
* Value obtained using System Test.
† For fuse ratings, refer to Table A.3-2, Fuse Ratings and Functions.
** +300 Vdc must be turned ON to measure.
PN 4235961E A.1-9
Volumes
Aspiration Pump, Primary Mode
Table A.1-24 Aspiration Pump Volume Tolerances
Control Used X* Distance Tolerance Replicate Tolerance

Enhanced 5C Normal cell control† 13 mm ±3 mm (0.5 in. ±0.125 in.) ±7 mm (±0.25 in.)
4C PLUS Abnormal High cell control 25 mm ±3 mm (1 in. ±0.125 in.) ±7 mm (±0.25 in.)
* Measurement taken between the trailing edge of the sample and the end of the fitting on BD1-2
on the second pull.
† Has a high Hgb value.
Diluent Dispensers
Table A.1-25 Diluent Dispenser Volume Specifications and Tolerances
Tolerances*
Diluent Dispensers Specifications Measured by Volume Measured by Weight

RBC diluent dispenser (10 mL) 10 mL ±0.05 mL 100 mL ±1.0 mL 10 g ±0.05 g
WBC diluent dispenser (6 mL) 6 mL ±0.03 mL 60 mL ±1.0 mL 6 g ±0.03 g
* Based on dispensing 10 times.
CBC Lytic Reagent Pumps’ Volume Tolerance

The total volume of CBC lytic reagent obtained by dispensing reagent 12 times must weigh
between 12.48 g and 12.96 g.
A.1-10 PN 4235961E
CIRCUIT CARDS - JUMPERS AND TEST POINTS A
A.2 CIRCUIT CARDS - JUMPERS AND TEST POINTS
376 CPU Card
Figure A.2-1 376 CPU Card Jumper Locations
X20 X18 X14 X21 X8 X1
X10
X7
X9
X6
X22 X5
(Referred to
as S2 when
switch is
removed)
X13
X3
X2
X11
X17 X15 X12 X4

X16
X19 5961186D
Jumper Settings
Table A.2-1 376 CPU Card Jumper Settings (See Figure A.2-1)
Jumper Setting Jumper Setting Jumper Setting

X1 No jumper X8 Pins 2 and 3 left side X15 No jumper
X2 Jumper installed* X9 No jumper X16 No jumper
X3 Jumper installed* X10 Pins 2 and 3 right side X17 Jumper installed
X4 Jumper installed* X11 Jumper installed X18 No jumper
X5 Pins 1 and 3 right side X12 Pins 1 and 3 left side X19 Pins 2 and 3 right side
X6 Pins 1 and 3 right side X13 Pins 2 and 3 right side X20 No jumper
X7 Pins 1 and 3 right side X14 Pins 2 and 3 left side X21 Pins 1 and 3 right side
X22† Pins 1 and 3 right side
* Installing jumpers X2, X3 and X4 enables the on-board battery circuits.
† Referred to as S2 when switch is removed. When switch is present, there is nothing to jumper at X22.
PN 4235961E A.2-1
CIRCUIT CARDS - JUMPERS AND TEST POINTS
Autoloader Interface Card

Figure A.2-2 Autoloader Interface Component Locations
J1 J2 J3 J4
J6
J7
J5
J8
J11
J9
SW2
(LV82)
J10
SW3
(LV83)
J12
J13
J14
J15
J16
1713448A
5961254D
J17 J18 J19
Connectors
Table A.2-2 Autoloader Interface Card Connectors (See Figure A.2-2)
J No. Connects to: J No. Connects to:

J1 Not used J11 LV82, pierce needle solenoid
J2 S15, load stack empty sensor J12 S16, (rocker bed) pierce position sensor
J3 S10, tube-ram sensor J13 S17, (rocker bed) horizontal position sensor
J4 S11, unload stack full sensor J14 S0, needle home sensor
J5 LV81, tube-return solenoid J15 S1, needle forward sensor
J6 LV84, bed-lock solenoid J16 S14, tube forward sensor
J7 Not used J17 S4, STOP switch
J8 Not used J18 S5, door interlock switch
J9 Sample Handler card, J15 J19 M1, rocker bed motor
Note: P16 connects to J9, P15 to J15.
J10 LV83, tube ram solenoid
A.2-2 PN 4235961E
DILUTER INTERFACE Card
Figure A.2-3 DILUTER INTERFACE Card Component Locations
X2
LYSE TRIG
(TP26)
5961191D
Jumper Setting
X2 - Jumper pins 2 and 3, left side (See Figure A.2-3).
Test Point
TP26, LYSE TRIG (See Figure A.2-3) - used when performing diluent and lytic reagent timing
PN 4235961E A.2-3
Fluid Detector/Ram Pressure Card

Figure A.2-4 Fluid Detector/Ram Pressure Card Component Locations
J9
J3 J8
J4 J7
J5 J6
LEDs
(1 to10 1713039C
right to left)
J2
J1
5961253D
Connectors
Table A.2-3 Fluid Detector/Ram Pressure Card Connectors (See Figure A.2-4)

J1 P7, signal cable to analog backplane, TB1 and TB3 J6 P86, diluent optical sensor, FD6
J2 P91, signals from Solenoid Junction card for LV10, J7 P87, cleaning agent optical sensor, FD7
LV11, LV12 and LV13 (the prime sensor solenoids)
J3 P83, CBC lytic reagent optical sensor, FD3 J8 P92, signals to LV10, LV11, LV12 and LV13 (the
prime sensor solenoids)
J4 P84, Erythrolyse II optical sensor, FD4 J9 Not used.
J5 P85, StabiLyse optical sensor, FD5
LEDs
Table A.2-4 Fluid Detector/Ram Pressure Card LED Positions (See Figure A.2-4)
LED Light goes out when: LED Light goes out when: LED Light goes out when:
1 RBC bath overflows. 5 StabiLyse is empty. 8 Spare
2 WBC bath overflows. 6 Diluent is empty. 9 Spare
3 CBC lytic reagent is empty. 7 Cleaning agent is empty. 10 Not used.
4 Erythrolyse II is empty.
A.2-4 PN 4235961E
I/O Card
Figure A.2-5 I/O Card Jumper Locations
X2
X1
5961190C
Jumper Settings
X1 - No jumper (See Figure A.2-5)
X2 - Jumper pins 2 and 3, left side (See Figure A.2-5).
PN 4235961E A.2-5
Peltier Controller Card

Figure A.2-6 Peltier Controller Card Connector and LED Locations
J2
J1
J3
CR1 CR3 CR2 5961229D

(Not ready) (Cooling) (Heating)
Connectors and LEDs
Table A.2-5 Peltier Controller Card Connectors and LEDs (See Figure A.2-6)
Connectors LEDs
J No. Function CR No. Function

J1 P5, power from the Electronic Power Supply via CR1 Not ready, stabilizing
the terminal board.
r Pin 1 = ground
r Pin 2 = +24 Vdc
r Pin 3 = +12 Vdc
J2 P4, 16-pin signal cable from analog backplane CR2 Heating
J3 P3, cooling fan CR3 Cooling
A.2-6 PN 4235961E
Pneumatic Monitor Card
Figure A.2-7 Pneumatic Monitor Card Component Locations
TP100 TP200 TP300 TP400 TP500 TP600
J1
30 psi
transducer
60 psi
transducer
High vacuum
transducer
Sheath
pressure
transducer
Diff
Low vacuum
pressure
transducer
transducer
5961230D
Connector
J1 connects to P1 from the analog backplane, TB1 and TB3
PN 4235961E A.2-7
Test Points
Table A.2-6 Pneumatic Monitor Card Pneumatic Reading to Vdc Conversion (See Figure A.2-7)
TP100 - 30 psi TP200 - 60 psi TP300 - Hi Vac TP400 - Sheath TP500 - Lo Vac TP600 - Diff
psi Vdc psi Vdc in. Hg Vdc psi Vdc in. Hg Vdc psi Vdc
0 0 0 0 0 0 0 0 5 0 0 0
1 0.286 2 2.286 1 0.333 1 0.714 5.25 1.25 0.1 0.667
2 0.571 4 0.571 2 0.666 2 1.429 5.50 2.50 0.2 1.333
3 0.857 6 0.857 3 0.999 3 2.143 5.75 3.75 0.3 2.000
4 1.143 8 1.142 4 1.332 4 2.857 6.00 5.00 0.4 2.666
5 1.429 10 1.428 5 1.665 5 3.572 6.25 6.25 0.5 3.333
6 1.714 12 1.714 6 1.998 6 4.286 6.50 7.50 0.6 3.999
7 2.000 14 1.999 7 2.331 7 5.000 6.75 8.75 0.7 4.666
8 2.286 16 2.285 8 2.664 8 5.715 7.00 10.00 0.8 5.333
9 2.571 18 2.570 9 2.997 9 6.429 0.9 5.999
10 2.857 20 2.856 10 3.330 10 7.143 1.0 6.666
11 3.143 22 3.142 11 3.663 11 7.857 1.1 7.332
12 3.428 24 3.427 12 3.996 12 8.572 1.2 7.999
13 3.714 26 3.713 13 4.329 13 9.286 1.3 8.665
14 4.000 28 3.998 14 4.662 14 10.00 1.4 9.332
15 4.286 30 4.284 15 4.995 1.5 9.998
16 4.571 32 4.570 16 5.328
17 4.857 34 4.855 17 5.661
18 5.143 36 5.141 18 5.994
19 5.428 38 5.426 19 6.327
20 5.714 40 5.712 20 6.660
21 6.000 42 5.998 21 6.993
22 6.285 44 6.283 22 7.326
23 6.571 46 6.569 23 7.659
24 6.857 48 6.854 24 7.992
25 7.143 50 7.140 25 8.325
26 7.428 52 7.425 26 8.685
27 7.714 54 7.711 27 8.991
28 8.000 56 7.997 28 9.324
29 8.285 58 8.282 29 9.657
30 8.571 60 8.568 30 9.990
31 8.857 62 8.854
32 9.142 64 9.139
33 9.428 66 9.428
34 9.714 68 9.710
35 10.000 70 9.996
A.2-8 PN 4235961E
RAM Timer Card
Figure A.2-8 RAM Timer Card Jumper Locations
376 CPU card

RAM Timer card
X1
X2
5961187C
Jumper Settings
X1 - Jumper pins 1 and 3, left side (See Figure A.2-8)
X2 - No jumper (See Figure A.2-8)
PN 4235961E A.2-9
Rocker Bed Interface Card

Figure A.2-9 Rocker Bed Interface Card Connector Locations
J42
J43 J41
J39
J38
J40
J37 J35
J32
J34 J33
1712958A
5961255D
J31
Connectors
Table A.2-7 Rocker Bed Interface Card Connectors (See Figure A.2-9)

J31 Autoloader Interface card, J17 J38 S12, cassette position 0, RED/ORN/BRN
J32 B2, unload elevator motor (left) J39 S9, full cass index rotation, RED/GRN/WHT
J33 B1, load elevator motor (right) J40 S3, cassette position 2, RED/ORN/BRN
J34 M2, cassette index motor J41 S2, cassette position 1, RED/ORN/BRN
J35 S8, load elevator down (right), RED/GRN/WHT J42 S7, tube in position, RED/YEL/WHT
J37 S6, unload elevator down (left), RED/GRN/WHT J43 S13, cassette position 3, RED/ORN/BRN
A.2-10 PN 4235961E
R/W/P PROC Card
Figure A.2-10 R/W/P PROC Card Component Locations
E14 E8
E2 E3
E15 E10 E7 E5
E9
E31
E30
WHT
E26
E28
E27
PLT
RED
GND
E23
E22
E16 E11 5961231D

E20 E18 E6 E4
E17 E13
E19
E12
Jumper Settings
Table A.2-8 R/W/P PROC Card Jumper Settings (See Figure A.2-10)
Jumper pin... To pin... Jumper pin... To pin...

E2 E3 E16 E17
E4 E6 E18 E19
E5 E7 E22 E23
E8 E10 E26 E27
E11 E13 E30 E31
E14 E15
Test Points
Table A.2-9 R/W/P PROC Card Test Points (See Figure A.2-10)
TP Ground Function
WHT GND Allows measurement of noise on the WBC channel.
PLT GND Allows measurement of noise on the Plt channel.
RED GND Allows measurement of noise on the RBC channel.
PN 4235961E A.2-11
Sample Handler Cards
Figure A.2-11 Sample Handler I Card Blood/Bubble Detector Adjustments and Test
Component Locations Points - Sample Handler I Card
X26 X27 X15 X13 X14 X12 J3 J2 Front blood/bubble detector
X25 X10 r Voltage reading - TP23 (See Table A.1-22,

X24 for expected readings and limits.)
J5
J6 r Adjustment - R64
X21 TP5
X20 Rear blood/bubble detector
X23 J1
X22 r Voltage reading - TP19 (See Table A.1-22,
X11
J15 for expected readings and limits.)
CR4 CPU
X19 CR3 ROM r Adjustment - R77
X18 CR2 RAM
CR1 I/O
X17
X16
TP23
R77 X7
X1
J13
X6
J14 X5
X4
R64
X2
5961234D TP19 X9 X8 X3
Connectors - Sample Handler I Card
Table A.2-10 Sample Handler I Card Connectors (See Figure A.2-11)

J1 P33, from P22 on the Solenoid Junction card J13 P49, from front blood/bubble detector
J2 P2, from the Electronic Power Supply via TB1, TB2, J14 P50 from rear blood/bubble detector
TB3, TB4.
J3 P3, from the Electronic Power Supply via TB2, TB3, J15 P15, from P16/J9 on the Autoloader Interface card
and TB4. (MAXM analyzer with Autoloader module)
J5 P43, from P44/P6 on the Bar-Code Decoder card P15, from P16/J3 on the RCP II Junction card
(MAXM analyzer with Rotary Cap-Pierce module)
J6 Not used
A.2-12 PN 4235961E
Jumper Settings - Sample Handler I Card
Table A.2-11 Sample Handler I Card Jumper Settings (See Figure A.2-11)
Jumper Setting Jumper Setting Jumper Setting

X1 No jumper X10 Jumper installed X19 Jumper installed
X2 Jumper installed X11 Jumper installed X20 No jumper
X3 Jumper installed X12 No jumper X21 Jumper installed, left side
X4 Jumper installed X13 Jumper installed X22 Jumper installed
X5 Jumper installed X14 Jumper installed, left side X23 No jumper
X6 Jumper installed X15 No jumper X24 No jumper
X7 No jumper X16 No jumper X25 No jumper
LEDs - Sample Handler I Card
Table A.2-12 Sample Handler I Card LED Functions (See Figure A.2-11)
LEDs
Operation Condition CPU ROM RAM I/O

System Beginning of on-board tests On On On On
power up
CPU test passed self test Off On On On
ROM passed self test Off Off On On
RAM passed self test Off Off Off On
I/O test passed self test Off Off Off Off
Software Waiting for download Off Off Off Flashes
download
I/O receiving download from DMS and placing code in RAM Off Off Flashes Flashes
Attempting to abort download On Off Off On
Lower door opened during or after download Flashes Off Off Off
After download, a sensor in the Rotary Cap-Pierce module is Off Off Off Off
misaligned or defective.
Note: The LED on the front of the lower cover lights.
Carousel rotation Carousel at HOME position. On Off Off Off
(Rotary
Carousel between HOME and PIERCE positions. Off On Off Off
Cap-Pierce
module only) Carousel at PIERCE position Off Off On Off
Carousel between PIERCE and HOME positions. Off Off Off On
PN 4235961E A.2-13
Figure A.2-12 Sample Handler II Card Blood/Bubble Detector Adjustments and Test
Component Locations Points- Sample Handler II Card
X32 X31 X28 J3 J2 Front blood/bubble detector
r Voltage reading - TP23 (See Table A.1-22,

J5
TP5 for expected readings and limits.)
X29 r Adjustment - R64
X30 Rear blood/bubble detector

J1
r Voltage reading - TP19 (See Table A.1-22,

CR4 CPU for expected readings and limits.)
J15
CR3 ROM
CR2 RAM r Adjustment - R77
U35 CR1 I/O
X33
X34
TP23
R77
J13
TP19
J14
R64
5961233D
Connectors - Sample Handler II Card
Table A.2-13 Sample Handler II Card Connectors (See Figure A.2-12)

J1 P33, from P22 on the Solenoid Junction card J13 P49, from front blood/bubble detector
J2 P2, from the Electronic Power Supply via TB1, TB2, J14 P50 from rear blood/bubble detector
TB3, TB4.
J3 P3, from the Electronic Power Supply via TB2, TB3, J15 P15, from P16/J9 on the Autoloader Interface card
and TB4. (MAXM analyzer with Autoloader module)
J5 P43, from P44/P6 on the Bar-Code Decoder card P15, from P16/J3 on the RCP II Junction card
(MAXM analyzer with Rotary Cap-Pierce module)
Jumper Settings- Sample Handler II Card
Table A.2-14 Sample Handler II Card Jumper Settings (See Figure A.2-12)
Jumper Setting Jumper Setting

X28 Jumper installed, left side X32 Jumper installed
X29 Jumper installed, left side X33 Jumper installed, left side
X30 Jumper installed X34 Jumper installed, left side
X31 Jumper installed
A.2-14 PN 4235961E
LEDs- Sample Handler II Card
Table A.2-15 Sample Handler II Card LED Functions (See Figure A.2-12)
LEDs
Operation Condition CPU ROM RAM I/O

System Beginning of on-board tests On On On On
power up
CPU test passed self test Off On On On
ROM passed self test Off Off On On
RAM passed self test Off Off Off On
I/O test passed self test Off Off Off Off
Software Waiting for download Off Off Off Flashes
download I/O receiving download from DMS and placing code in RAM Off Off Flashes Flashes
Attempting to abort download On Off Off On
Lower door opened during or after download Flashes Off Off Off
After download, a sensor in the Rotary Cap-Pierce module is Off Off Off Off
misaligned or defective.
Note: The red LED near the tube entry port lights.
Carousel rotation Carousel at HOME position. On Off Off Off
(Rotary
Carousel between HOME and PIERCE positions. Off On Off Off
Cap-Pierce
module only) Carousel at PIERCE position Off Off On Off
Carousel between PIERCE and HOME positions. Off Off Off On
PN 4235961E A.2-15
VCS PROCESSOR Card

Figure A.2-13 VCS PROCESSOR Card Component Locations
X1
V
C
GND
5961232D
Jumper Setting
X1 - Jumper pins 2 and 3, bottom (See Figure A.2-13)
Test Points
Table A.2-16 VCS PROCESSOR Card Test Points (See Figure A.2-13)
TP Ground Function
V GND Allows measurement of noise on the volume (DC) channel.
C GND Allows measurement of noise on the conductivity (RF) channel.
S GND Allows measurement of noise on the light scatter (LS) channel.
A.2-16 PN 4235961E
POWER SUPPLIES - SWITCH SETTINGS; FUSE RATINGS; AND CONNECTIONS, JUMPERS, AND TEST POINTS A
A.3 POWER SUPPLIES - SWITCH SETTINGS; FUSE RATINGS; AND CONNECTIONS,
JUMPERS, AND TEST POINTS
Power Switch Settings
Table A.3-1 Input Power Switch Settings
Switch Setting Input Voltage Frequency

120 Vac 90 -132 Vac 48 - 62 Hz
220 Vac 198 - 242 Vac 48 - 62 Hz
Fuse Ratings and Functions

Table A.3-2 Fuse Ratings and Functions
Fuse Fuse Rating - 220/240 Vac Fuse Rating - 100/120 Vac Function
1 3.2 A 6.25 A Main power
2 3.2 A 6.25 A Main power
3 1/4 A 1/2 A PS7 (5.6 V supply)
4 2A 4A PS3 (+5, +15, -15 V supply)
5 1A 2A PS4 (24 V supply)
6 1A 2A PS6 (12 V supply)
7 1/16 A 1/8 A Transformer T4 (240 Vdc)
8 1/8 A 1/4 A Transformer T3 (300 Vdc)
9 0.3 A SLO BLO 0.4 A SLO BLO Laser Power Supply
10 1.5 A SLO BLO 2.5 A SLO BLO Pneumatic Power Supply
PN 4235961E A.3-1
POWER SUPPLIES - SWITCH SETTINGS; FUSE RATINGS; AND CONNECTIONS, JUMPERS, AND TEST POINTS
Jumpers and Connections
Table A.3-3 Electronic Power Supply Terminal Card Jumpers and Connections
Voltage Terminal Cards Jumpers Wire Connections

96 to 106 Vac TB2, TB3, TB4, TB5 1 to 2 TB pos 1 - Brown
(120/220 selection switch to 120) 3 to 4 TB pos 5 - Blue
TB6, TB7 1 to 2 TB pos 1 - Brown
under rear cover) 5 to 6 TB pos 5 - Blue
(120/220 selection switch to 120) 3 to 4 TB pos 4 - Blue
(under rear cover) 3 to 4 TB pos 4 - Blue
(120/220 selection switch to 220) TB pos 5 - Blue
(under rear cover) TB pos 5 - Blue
(120/220 selection switch to 220) TB pos 4 - Blue
(under rear cover) TB pos 4 - Blue
Test Points and Adjustments
Table A.3-4 Electronic Power Supply Test Points and Adjustments
Connector Pins Voltage Adjustment

J105 1, 2, 3, 4 +5 Vdc PS3, R10
5, 6, 7, 8 +5 V Common
9, 10 +15 Vdc PS3, R27
11, 12 -15 Vdc PS3, R31
13, 14, 15 ±15 V Common
J107 1, 2, 3 +12 Vdc PS6, R11
4, 5, 6 +12 V Common
A.3-2 PN 4235961E
POWER SUPPLIES - SWITCH SETTINGS; FUSE RATINGS; AND CONNECTIONS, JUMPERS, AND TEST POINTS A
Table A.3-4 Electronic Power Supply Test Points and Adjustments (Continued)
Connector Pins Voltage Adjustment

J108 1, 2 +5.6 Vdc PS7, R6
3, 4 +5.6 V Common
5 Ready/Standby Switch Common
6 Ready/Standby Switch +5.6 Vdc (or Ov. with jumper)
7 Chassis Ground
8 Not connected
9 +6.3 Vdc Reference
10 To Ground Bus Bar
11 +240 V Common
12 +240 Vdc
J112 1 RF ON signal
2 Compressor ON
3 300 Vdc Reference
4 ACLO (power fail signal)
5 Standby LED
6 Standby LED
7 Ready LED
8 Ready LED
9 Not connected
J113/J114 1 Ac Line
2 Ac Neutral
3 Chassis Ground
J115 1 +300 Vdc RF Power Supply, R2
Note: Turn +300 Vdc ON to measure.
2 +300 V Common
3 +6.3 Vdc RF Power Supply, R1
4 +6.3 V Common
J116 1, 2 K3 (+24 Vdc)
3, 4, 5 +24 Vdc PS4, R11
6, 7, 8, 9, 10 +24 V Common
11, 12 Not connected
J119 (spare) 1 +15 Vdc PS3, R27
2 -15 Vdc PS3, R31
3 ±15 Vdc Common
J120 Refer to Table A.1-23, System Voltage Ranges.
PN 4235961E A.3-3
POWER SUPPLIES - SWITCH SETTINGS; FUSE RATINGS; AND CONNECTIONS, JUMPERS, AND TEST POINTS
Laser Power Supply
Table A.3-5 Laser Power Supply Buck-Boost Transformer Jumpers and Connections
Voltage Jumpers Wire Connections

90 to 106 Vac None TB pos 4 - White/Brown
TB pos 1 - Brown
107 to 119 Vac None TB pos 4 - Brown
(Buck-Boost not required) TB pos 4 - White/Brown
120 to 132 Vac None TB pos 3 - White/Brown
TB pos 4 - Brown
198 to 219 Vac 1 to 2 TB pos 4 - White/Brown
3 to 4 TB pos 1 - Brown
220 to 232 Vac 1 to 2 TB pos 3 - Brown
(Buck-Boost not required) 3 to 4 TB pos 4 - White/Brown
233 to 264 Vac 1 to 2 TB pos 1 - White/Brown
3 to 4 TB pos 4 - Brown
Pneumatic Power Supply
Table A.3-6 Pneumatic Power Supply Buck-Boost Transformer Terminal Card Jumpers and Connections
Voltage Jumpers Wire Connections

90 to 106 Vac None TB pos 1 and 5 - Black (fan)
TB pos 1 - Black (compressor)
TB pos 4 - White/Brown
(Buck-Boost not required) TB pos 4 - Black (compressor)
TB pos 4 - Black (compressor)
198 to 219 Vac 1 to 2 TB pos 1 and 5 - Black (fan)
3 to 4 TB pos 1 - Black (compressor)
(Buck-Boost not required) 3 to 4 TB pos 4 - Black (compressor)
3 to 4 TB pos 4 - Black (compressor)
A.3-4 PN 4235961E
SOFTWARE SETTINGS A
A.4 SOFTWARE SETTINGS
Bar-Code Reader Decoder Card
Table A.4-1 Bar-Code Reader Decoder Card Code Types Default Settings
Setting CODE 39 CODABAR I 2-of-5 UPC CODE 128

Code Type Enabled Enabled Enabled Disabled Enabled
Fixed Length Disabled Disabled N/A N/A Disabled
Code Length #1 10.00 10 12 N/A 10
Code Length #2 N/A N/A 0 N/A N/A
Check Digit Enabled Enabled Enabled N/A N/A
C/D Output Disabled Disabled Disabled N/A N/A
C/D Aim* N/A Enabled N/A N/A N/A
C/D Type* N/A AIM-16 N/A N/A N/A
Interchar Gap Disabled Disabled N/A N/A N/A
S/S Match N/A Disabled N/A N/A N/A
S/S Output N/A Disabled N/A N/A N/A
EAN N/A N/A N/A Disabled N/A
Narrow Margins† Disabled Disabled Disabled Disabled Disabled
* Only one of these two setting titles is displayed. Use the default settings for the one displayed.
† If the customer is not using tube labels and the system cannot read the cassette label, change
setting to enabled.
Table A.4-2 Bar-Code Reader Decoder Card Communications Default Settings
Host Protocol Parameter Host Port Aux Port

Protocol = P-T-P W/XON XOFF Baud Rate 1200 1200
Preamble = DISABLED = <NULL><NULL> Parity Odd Odd
Postamble = ENABLED = <CR><LF> Stop Bits One One
Address = Data Bits Eight Eight
RES = <NULL> RS-422 Disabled Disabled
REQ = <NULL> Aux Mode N/A Full Duplex
EOT = <NULL>
STX = <NULL>
ETX = <NULL>
ACK = <NULL>
NAK = <NULL>
LRC = Enabled
Response Timeout = 12 ms
Intechar Delay = 0 ms
PN 4235961E A.4-1
SOFTWARE SETTINGS
Table A.4-3 Bar-Code Reader Decoder Card Operations Default Settings
Setting
Operation Rotary Cap-Pierce Module Autoloader Module

Triggering Mode = Serial Serial
End of Read Cycle = Timeout Timeout
Timeout in 10 ms incs = 1000 ms 1500 ms
Triggering Character = <GS > <GS >
External Trigger = N/A N/A
Noread Message = Noread Noread
Noread Message = Enabled Enabled
Barcode Output = Enabled Enabled
When to Output = As soon as possible As soon as possible
Good Decode Reads = 7 4
Match Code = Disabled Disabled
Number of Labels = One Two
Table A.4-4 Bar-Code Reader Decoder Card User Outputs Default Settings
Setting
User Output Rotary Cap-Pierce Module Autoloader Module

Beeper = On Good Disabled
Beeper Volume = Level 4 Level 4
Full Screens = Enabled Enabled
Relay Driver = Mismatch or Noread Mismatch or Noread
New Master Pin = Disabled Disabled
Good/Bad Polarity = Positive Positive
Good/Bad Pulse Width= 50 ms 50 ms
Gain Control = 0 0
A.4-2 PN 4235961E
DILUTER COMPONENT LOCATIONS AND FUNCTIONS A
A.5 DILUTER COMPONENT LOCATIONS AND FUNCTIONS
Most of the Diluter functions are accomplished by fluidic components that are interconnected
by tubing and controlled by timed solenoid signals.
r Table A.5-1 lists the main fluidic components in the Diluter in alphabetic order by
reference designator.
r Figures A.5-1 through Figure A.5-10 show the locations of the fluidic components in the
BSV, CBC, Main Diluter, Mixing Chamber, and Pump modules.
The components in the Flow-Cell module are discussed and illustrated under Heading 2.13,
SAMPLE ANALYSIS SYSTEM - VCS TECHNOLOGY.
To locate a specific component in the Diluter, refer to the Location Reference column in
Table A.5-1.
Table A.5-1 Diluter Component Location References and Functions
Reference Location
Designator Name Reference Functions
Ambient temperature Figure A.5-1 Provides the ambient temperature input for the Peltier module.
sensor Figure A.5-2
Aperture viewing Figure A.5-4 Allows operator to monitor apertures for debris.
screens
Aspirator tip Figure A.5-1 Input pathway for sample aspiration in the Secondary mode.
Figure A.5-2
Bleach probe Figure A.5-1 Input pathway for the bleach solution in the Disinfect cycle.
Figure A.5-2
BSV actuator Figure A.5-2 Rotates the left and center sections of the BSV.
Fluid Detector/Ram Figure A.5-7 Monitors the fluid detector sensors FD1 through FD7. See Fluid
Pressure card Detector/Ram Pressure Card under Heading 2.6, REAGENT
SYSTEM.
Hgb lamp Figure A.5-4 Light source for photometric measurement of Hgb.
Hgb Preamp module Figure A.5-4 Filters the light transmitted through the WBC bath, measures
the light as current, amplifies the signal, and converts it to
voltage. See Determining the Hgb under Heading 2.12, SAMPLE
ANALYSIS SYSTEM - CBC TECHNOLOGY.
Laser Figure 2.13-1 Coherent light source for light scatter measurement.
Lens block Figure 2.13-1 Focuses the laser light on the aperture in the flow cell.
LS Preamp module Figure 2.13-1 Receives and amplifies pulses from the LS sensor.
LS sensor Figure 2.13-1 Detects the light scattered as the WBCs pass through the
aperture in the flow cell.
Optic lamps Figure A.5-4 Lights aperture areas for viewing on the aperture viewing
screens.
Peltier Controller card Figure A.5-3 Controls the heating and cooling of the Peltier cells.
PN 4235961E A.5-1
DILUTER COMPONENT LOCATIONS AND FUNCTIONS
Table A.5-1 Diluter Component Location References and Functions (Continued)
Reference Location
Pneumatic Monitor Figure A.5-7 Monitors the system vacuums and pressures. See Pneumatic
card Monitor Card under Heading 2.5, PNEUMATIC SYSTEM.
RF Detector Preamp Figure 2.13-1 Generates the RF and DC current applied to the flow cell, detects
card RF voltage (to monitor for clogs in the flow cell), and amplifies
the RF and DC pulses from the flow cell. See RF Detector
Preamp Card under Heading 2.13, SAMPLE ANALYSIS SYSTEM
- VCS TECHNOLOGY.
BD1 Blood/bubble Figure A.5-1 One of a pair of blood/bubble detectors that monitor the fluid in
detector (front) Figure A.5-2 the aspiration line in the Primary mode. See Blood/Bubble
Detectors under Heading 2.11, SAMPLE PROCESSING SYSTEM
- DILUTER.
BD2 Blood/bubble Figure A.5-1 One of a pair of blood/bubble detectors that monitor the fluid in
detector (rear) Figure A.5-2 the aspiration line in the Primary mode. See Blood/Bubble
Detectors under Heading 2.11, SAMPLE PROCESSING SYSTEM
- DILUTER.
BSV1 Blood sampling valve Figure A.5-1 Isolates blood for the RBC/Plt and WBC/Hgb dilutions, and
Figure A.5-2 routes the blood samples, diluent and rinse to the RBC and WBC
baths, BT1 and BT2. See Blood Sampling Valve (BSV) under
Heading 2.11, SAMPLE PROCESSING SYSTEM - DILUTER
BT1 WBC bath Figure A.5-4 Holds WBC dilution for mixing and for collection of WBC and
Hgb data.
BT2 RBC bath Figure A.5-4 Holds RBC dilution for mixing and for collection of RBC and Plt
data.
CL1 CL1 Figure A.5-1 Rotates left section of BSV.
CL2 CL2 Figure A.5-1 Rotates center section of BSV.
CL3 CL3 Figure A.5-3 Moves the rinse block.
FC1 Flow cell Figure 2.13-1 Houses and provides path to VCS aperture.
FD1 RBC bath overflow Figure A.5-5 Monitors the overflow line from the RBC bath for liquid.
detector
FD2 WBC bath overflow Figure A.5-5 Monitors the overflow line from the WBC bath for liquid.
detector
FD3 Erythrolyse (PAK Figure A.5-7 Monitors the Erythrolyse II reagent supply.
LYSE) reagent
sensor
FD4 StabiLyse (PAK Figure A.5-7 Monitors the StabiLyse reagent supply.
PRESERVE)
reagent sensor
FD5 CBC lyse sensor Figure A.5-7 Monitors the CBC lytic reagent supply.
FD6 Diluent sensor Figure A.5-7 Monitors the diluent reagent supply.
A.5-2 PN 4235961E
Reference Location
FD7 Cleaning agent Figure A.5-7 Monitors the cleaning agent supply.
sensor
FL1 Vacuum trap Figure A.5-7 Traps any liquid in the vacuum lines, preventing the liquid from
traveling back to the pump in the Pneumatic Power Supply.
FL2 Water trap (air/water Figure A.5-7 Collects condensation from the pressurized air.
separator)
FMT1 Foam trap (vent Figure A.5-10 Collects any foam or liquid overflow from the vent waste
waste chamber) Figure A.5-11 chamber, VC3.
FMT2 Foam trap (diff waste Figure A.5-7 Collects any foam or liquid overflow from the diff waste
chamber) chamber, VC7.
FMT3 Foam trap (waste Figure A.5-5 Collects any foam or liquid overflow from the waste chamber,
chamber) VC1.
FMT4 Foam trap Figure A.5-2 Acts as a buffer to prevent interaction between the aspiration
pumps’ exhaust lines venting to atmosphere.
LV1 - LV8 Figure A.5-5 For function, see Table 4.26-1, Solenoid Operations
LV10 - LV13 Figure A.5-7 For function, see Table 4.26-1, Solenoid Operations.
LV14, LV15 Figure A.5-3 For function, see Table 4.26-1, Solenoid Operations.
LV16 -LV19 Figure A.5-1 For function, see Table 4.26-1, Solenoid Operations.
Figure A.5-11
LV31 -LV34 Figure A.5-9 For function, see Table 4.26-1, Solenoid Operations.
LV43 Figure A.5-7 For function, see Table 4.26-1, Solenoid Operations.
LV43 Purge solenoid Figure A.5-7 For function, see Table 4.26-1, Solenoid Operations.
MC1 Mixing chamber, diff Figure A.5-8 Chamber for mixing and incubating blood and reagents for diff
analysis.
MF1 Figure A.5-5 Contains solenoids LV1 through LV6 on the CBC module.
MF2 Figure A.5-1 Contains solenoids LV16 through LV19 on the BSV module.
PN 4235961E A.5-3
Reference Location
MF3 Figure A.5-3 Contains solenoids, LV14, LV15, LV 20 through 22 and LV53 on
the BSV module.
MF5 Figure A.5-10 Contains solenoids LV25 through LV30 on the Pump module.
Figure A.5-11
MF6 Figure A.5-10 Distributes 30-psi pressure within the Pump module.
Figure A.5-11
MF7 Figure A.5-10 Distributes vacuum within the Pump module.
Figure A.5-11
MF8 Figure A.5-5 Contains solenoids LV7 and LV8 on the CBC module.
MF10 Figure A.5-7 Contains solenoids LV35 through LV52, LV57 and LV58 on the
Main Diluter module.
MF11 Figure A.5-7 Distributes pressurized diluent in the Main Diluter module.
MF14 Figure A.5-7 Distributes 30-psi pressure within the Main Diluter module.
MF15 Figure A.5-9 Contains solenoids LV31 through LV34 and LV54 through 59 in
the Mixing module.
MF17 Figure A.5-7 Contains solenoids LV10 through LV13 in the Main Diluter
module.
PLB1 Peltier module Figure A.5-3 Heats or cools the mixing chamber rinse and the Erythrolyse II
reagent. See Reagent Temperature Control under Heading 2.6,
REAGENT SYSTEM.
PM1 CBC lytic reagent Figure A.5-4 One of a pair of reagent pumps that deliver CBC lytic reagent to
pumps WBC bath, BT1, to lyse RBCs and react with the hemoglobin.
PM2 CBC lytic reagent Figure A.5-4 One of a pair of reagent pumps that deliver CBC lytic reagent to
pumps WBC bath, BT1, to lyse RBCs and react with the hemoglobin.
PM3 Air pump Figure A.5-1 In the Diff mode, pushes the sample for the Diff dilution out of
Figure A.5-2 the BSV and into the Erythrolyse II reagent input line to the
mixing chamber, MC1.
PM4 Primary-mode Figure A.5-2 Aspirates 185 µL of whole blood from the specimen tube via the
aspiration pump Figure A.5-10 needle assembly in the Primary mode.
PM5 Secondary-mode Figure A.5-2 Aspirates 125 µL of whole blood from the specimen tube via the
aspiration pump Figure A.5-10 aspirator tip in the Secondary mode.
PM6 Erythrolyse II reagent Figure A.5-10 One of a pair of reagent pumps that dispense Erythrolyse II
pump Figure A.5-11 reagent (diff lytic reagent) through the Peltier module to the
PM7 Erythrolyse II reagent Figure A.5-10 One of a pair of reagent pumps that dispense Erythrolyse II
pump Figure A.5-11 reagent (diff lytic reagent) through the Peltier module to the
PM8 Backwash pump Figure A.5-7 Provides 3 mL diluent or cleaning agent to rinse the aspiration
pathways.
A.5-4 PN 4235961E
Reference Location
PM9 WBC diluent Figure A.5-7 Dispenses 6.0 mL diluent to WBC bath, BT1, for the WBC
dispenser dilution and to the RBC bath, BT2, to rinse the bath.
PM10 Cleaning agent pump Figure A.5-7 Delivers 5 mL cleaning agent to the flow cell, FC1, during flow
cell cleaning functions.
PM11 RBC diluent Figure A.5-7 Dispenses 10 mL diluent to the RBC bath, BT2, for the RBC
dispenser dilution and to the WBC bath, BT1, to rinse the bath.
PM12 StabiLyse reagent Figure A.5-8 Dispenses StabiLyse reagent (diff preservative) to the mixing
pump chamber, MC1.
PV1 - PV7 Figure A.5-4
PV8 Figure A.5-5

Figure A.5-2
Figure A.5-11
PV23 Figure A.5-2
Figure A.5-10
PV24 Figure A.5-10
Figure A.5-11
PV25 Figure A.5-2
Figure A.5-10
Figure A.5-11
PV49 Figure A.5-7
PV66 Figure A.5-5
PV67 Figure A.5-5
PV68, PV69 Figure A.5-4
PV70 Figure A.5-10

Figure A.5-10
RB1 Rinse block Figure A.5-1 Cleans the outside of the aspirator tip in the Secondary mode.
RG1 Vacuum (low) Figure A.5-7 Regulates the low (6 in. Hg) vacuum used to pull the dilutions
regulator through the RBC and WBC apertures.
RG2 30 psi regulator Figure A.5-7 Regulates high pressure to 30 psi.
PN 4235961E A.5-5
Reference Location
RG3 Sheath-pressure Figure A.5-7 Regulates pressure for sheath fluid.
regulator
RG4 Sample-pressure Figure A.5-7 Regulates pressure for diff sample flow through the flow cell.
regulator
SF1 Sweep-flow tank Figure A.5-4 Supplies the diluent that flows behind the RBC aperture to
sweep cells away as they exit the aperture, preventing the cells
from swirling back into the sensing area.
ST1 Sheath tank Figure A.5-1 Provides pressurized diluent for sheath flow through flow cell.
Figure A.5-2
VC1 Waste chamber Figure A.5-4 Collects the waste from the RBC and WBC baths (BT2 and BT1)
and the RBC and WBC vacuum isolator chambers (VC10 and
VC11).
VC2 Bubble trap Figure A.5-5 Traps bubbles and distributes diluent to the sweep-flow lines
VC3 Vent waste chamber Figure A.5-10 Collects waste from the needle vent chamber.
Figure A.5-11
VC4 Needle vent chamber Figure A.5-10 Collects any blood aspirated into the needle vent line and the
Figure A.5-11 diluent or cleaning agent used to clean the needle.
VC5 Bleach chamber Figure A.5-7 Provides vacuum to draw in bleach solution during a Disinfect
cycle and provides a path for venting vacuum from the system
when the compressor turns off.
VC6 Vacuum chamber Figure A.5-7 Distributes vacuum in the Diluter.
VC7 Diff waste chamber Figure A.5-7 Collects waste from the mixing chamber, MC1, and the flow cell,
FC1.
VC8 Overflow chamber Figure A.5-4 Collects any liquid that overflows from the RBC or WBC baths,
BT2 or BT1.
VC9 Vacuum tank Figure 2.5-2 Stabilizes the vacuum supply used by the aspiration pumps.
VC10 RBC vacuum isolator Figure A.5-4 Distributes low vacuum (6 in. Hg) to pull dilution through the
chamber RBC aperture and sweep-flow diluent behind the RBC bath, BT2.
VC11 WBC vacuum isolator Figure A.5-4 Distributes low vacuum (6 in. Hg) to pull dilution through the
chamber WBC aperture.
A.5-6 PN 4235961E
Figure A.5-1 BSV Module, Old Configuration, Front View
PV13
PV12
PV11 ST1
PV10
PM3
QD2M
PV15
PV14
QD3M
Bleach
probe
CL1
CL2
BD1
BD2 LV19
LV18
BSV1 LV17
RB1 LV16
MF2 Ambient
Aspirator tip
temperature
sensor
5961242D Sample bar
Figure A.5-2 BSV Module, New Configuration, Front View
PV10 PV11 PV12 PV13 PV14 (front) PV15 (rear)

FMT4
QD2M ST1
PV23 LV17
PV25
PM3
PM4
PM5
QD3M LV18
Bleach probe LV16
BD1 LV19
BD2
Ambient
temperature
BSV1 sensor
RB1
BSV
Aspirator tip
actuator
Sample bar
5961258E
PN 4235961E A.5-7
Figure A.5-3 BSV Module, Rear View
MF3
(LV14, LV15, LV20,
LV21, LV22, LV53
top to
bottom)
CL3
Peltier
Controller
card
PLB1
5961256D
Figure A.5-4 CBC Module, Front View

Aperture
viewing screens
VL1
PV3
PV2 PV4
VC1
PM1 and PM2
VC10 VC11
BT2
BT1
PV68
PV69 Hgb Preamp
module
VC8 Hgb lamp
Optic lamps PV6
PV5
5961243D PV7
A.5-8 PN 4235961E
Figure A.5-5 CBC Module, Rear View
PV66 PV67
Mirror
top
VC2 QD1M
SF1
FD1,
FMT3
FD2 (closest
to panel)
LV7 (closest
to panel),
LV8
P54
5961269D PV8 MF1 LV6 LV5 LV4 LV3 LV2 LV1 MF8
Figure A.5-6 Main Diluter Module, Front View
QD1F
RG1
PV31 PV32
PV30
PV36
PV35 PV37
PV33 CK 30
PV34
PV41
PV40 PV42
PV38
PV39
PV45 PV46
PV43
PV44
QD2F QD5F
QD6F
PV47
QD3F PV48
5961262D
PN 4235961E A.5-9
Figure A.5-7 Main Diluter Module, Right-Side View

VC6 VC5
PM11
Fluid
MF10 Detector/
(LV35-LV52, Ram
LV67, LV68, Pressure
top to bottom) card
RG1 Pneumatic
Monitor
PM9 card
PM10
RG2 PV63
LV43 PV62
PV64
FMT2 Top
view PV61
FL2 PV65
LV10
FL1 LV11
LV12
MF11 LV13
MF14 MF17
RG3
FD5
5961265D RG4 PM8 PV49 VC7 FD7 FD6 FD4 FD3
Figure A.5-8 Mixing Module, Front View

PV51 PV52 PV53 PV54
PV50 QD6M
P52
MCI
QD7
(WM2)
PM12
QD9
(WM4)
QD8
PV55 (WM3)
QD11
PV56 (WM6)
QD10
(WM5)
PV57
PV58 PV60
PV59 5961244D
A.5-10 PN 4235961E
Figure A.5-9 Mixing Module, Rear View
MF15 Mixing
motor
LV31
LV32
LV33
LV34
LV54
LV55
LV56
LV57
LV58
LV59 7535032A
Figure A.5-10 Pump Module, Old Configuration
VC3
MF5
(LV25-LV30,
right to left)
P53
PV70
1 PV20
VC4
QD5
PV24
PV23
PV22
FMT1
PV21
QD12F PV25
QD13F PV26
PV27
PV28
MF6, MF7 PM4 PM5 PM6 PM7 5961246D
PN 4235961E A.5-11
Figure A.5-11 Pump Module, New Configuration
VC3
MF5
(LV25-LV30,
right to left)
P53
PV70
1 PV20
VC4
QD5
PV24
PV22
FMT1
PV21
QD12F
QD13F PV26
PV27
PV28
MF6, MF7 PM6 PM7 5961295D
A.5-12 PN 4235961E
SOFTWARE MENU TREES A
A.6 SOFTWARE MENU TREES
Figure A.6-1 MAXM Analyzer with Rotary Cap-Pierce Module Software Menu Tree, Revision 8D
and Higher
SAMPLE ANALYSIS CONTROLS DILUTER FUNCTIONS SPECIAL FUNCTIONS
WORKLOAD RECORDING ** MULTIPLE APERTURE ZAP

RUN SAMPLES CONTROL RUN PRIME REAGENTS DIAGNOSTICS OPERATOR OPTIONS COMPRESSOR ON/OFF
DATABASE QUERY REVIEW OR REPORT START UP SET UP SERVICE OPTIONS FLUIDIC TESTS DISABLE REAGENT SENSORS
WORKLIST GRAPHS SHUT DOWN CALIBRATION BSV TESTS BUBBLE MIX
HOST WORKLIST DISINFECT ERROR FILE CVS FUNCTIONS CLEAN NEEDLE
(optional) SYSTEM TEST
XB SOLENOID TEST
DRAIN AND VENT CYCLE BSV
DILUENT REPRODUCIBILITY HGB LAMP ADJUST BLOOD DETECTOR TEST
CURRENT XB BATCH LYSE CARRYOVER PROBE WASH
XB BATCH MEANS PAK CBC CALIBRATION BSV REMOVAL
XB GRAPHS CLEANER ENTER CALIBRATION FACTORS BLOOD DETECTOR ON/OFF
ALL
CVS INITIALIZE
F3 FROM RUN SAMPLES SCREEN CYCLE CVS
CERTIFICATION
SAMPLE MODE? REPRODUCIBILITY LATEX CHECKS
CARRYOVER WBC HISTOGRAM CHECK
F2 START PRIMARY SWITCH/SENSOR CHECK RED & WHT CHANNEL CALIBRATION
F3 SECONDARY TEST PULSES MPV LATEX CALIBRATION
F4 PREDILUTE CBC ENTER SECONDARY CAL FACTORS DIFF LATEX CALIBRATION
* RAMP TEST RETIC LATEX CALIBRATION
F5 RETIC DOUBLE PREC. TEST INIT CAL FACTORS
F6 DIFF ON/OFF SOLENOID TEST
F7 PURGE RAW DATA
F8 RINSE PRIMARY SECONDARY MODE CAL
XB LIMITS
F9 STOP SECONDARY DEFINITIVE FLAG LIMITS
DIFF: ON/OFF HIGH/LOW FLAG LIMITS
BLOOD DET: ON/ INACTIVE LABORATORY NORMAL RANGES
SELECT TO CHANGE/ESC TO CONTINUE
CBC/DIFF FILE
LATEX FILE SCREEN LABELS
F5 FROM RUN SAMPLES SCREEN CBC FILE PARAMETER SELECTION
* REPORTING UNITS
RETIC FILE
F1 HELP AUTO STOP
F2 XB: ON N=0 IN
F3 CAP PIERCER 10mm 13mm
F4 DB: ON ACTION LIMITS
F5 PRINT: NONE CONTROL SET UP LOCATION LIST **
F6 HOST: OFF SAMPLE ANALYSIS SET UP PHYSICIAN LIST **
F7 DISPLAY ONLY: OFF SYSTEM SET UP DISPLAY FORMATS
F8 OPERATOR: OPR DELETE DATABASE
F11 B&W SCREEN PRINT DELETE HOST SPOOLER
F12 COLOR SCREEN PRINT CLEAR PRINTER SPOOLER QUEUE
PRINT OPTIONS
AUTO PRINT FORMAT

TICKET OPTIONS
SPOOLER PRIORITY
GRAPHICS OPTIONS
* THIS ITEM IS AVAILABLE WITH THE RETIC OPTIONS PACKAGE. OPTIONAL PRINTER
SHIFT
* * THESE ITEMS ARE AVAILABLE WITH THE DATA AND RETIC REAGENTS
INSTITUTION HOST COMPUTER
OPTIONS PACKAGES. COMMUNICATION DEF DEFINITION
LANGUAGE
IQAP ID #
SET DATE/TIME
SUPERVISOR PASSWORD
LYSE VOLUME
OPTUNE
5961217D
PN 4235961E A.6-1
SOFTWARE MENU TREES
Figure A.6-2 MAXM Analyzer with Autoloader Module Software Menu Tree, Revision 8D and Higher
SAMPLE ANALYSIS CONTROLS DILUTER FUNCTIONS SPECIAL FUNCTIONS
RUN SAMPLES
DATABASE QUERY
CONTROL RUN
REVIEW OR REPORT
PRIME REAGENTS
START UP
DIAGNOSTICS
SET UP
WORKLOAD RECORDING
OPERATOR OPTIONS ** MULTIPLE APERTURE ZAP
COMPRESSOR ON/OFF
DISABLE REAGENT SENSORS
SERVICE OPTIONS FLUIDIC TESTS
WORKLIST GRAPHS SHUT DOWN CALIBRATION BUBBLE MIX
HOST WORKLIST ERROR FILE BSV TESTS
DISINFECT CLEAN NEEDLE
(optional) AUTOLOADER TESTS
XB SYSTEM TEST
SOLENOID TEST
DRAIN AND VENT CYCLE BSV
DILUENT REPRODUCIBILITY
HGB LAMP ADJUST BLOOD DETECTOR TEST
LYSE CARRYOVER
CURRENT XB BATCH PROBE WASH
PAK CBC CALIBRATION
XB BATCH MEANS BSV REMOVAL
CLEANER ENTER CALIBRATION FACTORS
XB GRAPHS BLOOD DETECTOR ON/OFF
ALL
AUTOLOADER HOME
F3 FROM RUN SAMPLES SCREEN CLEAR THE BED/
AUTOLOADER HOME
SAMPLE MODE ? ROCK THE BED
RIGHT ELEVATOR UP / DOWN
F2 START PRIMARY LEFT ELEVATOR UP / DOWN
F3 SECONDARY AUTOLOADER TEST ROUTINE
F4 PREDILUTE CBC CERTIFICATION
F5
F6
RETIC
*
DIFF ON / OFF
REPRODUCIBILITY
CARRYOVER
LATEX CHECKS
WBC HISTOGRAM CHECK
RED & WHT CHANNEL CALIBRATION
F7 PURGE SWITCH/SENSOR CHECK
F8 RINSE TEST PULSES MPV LATEX CALIBRATION
F9 STOP ENTER SECONDARY CAL FACTORS DIFF LATEX CALIBRATION
RAMP TEST RETIC LATEX CALIBRATION
INIT CAL FACTORS
DIFF: ON / OFF DOUBLE PREC. TEST
SOLENOID TEST
BLOOD DET: ON / INACTIVE
RAW DATA
SELECT TO CHANGE / ESC TO CONTINUE
SECONDARY MODE CAL
PRIMARY
AUTOLOADER TESTS
SECONDARY
CASSETTE AND POSITION
F5 FROM RUN SAMPLES SCREEN
F1 HELP AUTOLOADER TEST ROUTINE

F2 XB: ON N=0 IN ONE POSITION CASSETTE ADVANCE
F4 DB: ON NEEDLE PIERCE TEST LOAD A CASSETTE
F5 PRINT: NONE LIFT AND LOAD/UNLOAD UNLOAD A CASSETTE
F6 HOST: OFF
F7 DISPLAY ONLY: OFF
F8 OPERATOR: OPR XB LIMITS
F11 B&W SCREEN PRINT DEFINITIVE FLAG LIMITS
F12 COLOR SCREEN PRINT HIGH/LOW FLAG LIMITS
LABORATORY NORMAL RANGES
CBC/DIFF FILE
SCREEN LABELS
LATEX FILE
PARAMETER SELECTION
CBC FILE
REPORTING UNITS
RETIC FILE
AUTO STOP *
ACTION LIMITS
CONTROL SET UP
SAMPLE ANALYSIS SET UP
LOCATION LIST
PHYSICIAN LIST **
**
SYSTEM SET UP DISPLAY FORMATS
DELETE DATABASE
DELETE HOST SPOOLER
CLEAR PRINTER SPOOLER QUEUE
PRINT OPTIONS
AUTO PRINT FORMAT

TICKET OPTIONS
SPOOLER PRIORITY
GRAPHICS OPTIONS
OPTIONAL PRINTER
SHIFT
REAGENTS
INSTITUTION
HOST COMPUTER
COMMUNICATION DEF
DEFINITION
LANGUAGE
IQAP ID #
*THIS ITEM IS AVAILABLE WITH THE RETIC OPTIONS PACKAGE. SET DATE/TIME
SUPERVISOR PASSWORD
**THESE ITEMS ARE AVAILABLE WITH THE DATA AND RETIC OPTIONS PACKAGES. LYSE VOLUME
OPTUNE
5961218D
A.6-2 PN 4235961E
SOFTWARE MENU TREES A
Figure A.6-3 Service Disk Software Utility Menu Tree
SERVICE PARAMETERS ONLY
BACKUP TO FLOPPY COMPLETE DATABASE
FILES GET FLOPPY BACKUP †
SELECT DATA SET †
5C PHASE II ASSAY
RETIC-C ASSAY
LATRON ASSAY
SETUP* LATRON
LATRON OFFSETS
PRINTER PORT
PRINTER SETUP
SERVICE PRINTER TYPE
DISK
GRAPH RANGE
UTILITIES
MENU
ENABLE MAXM SUSPECT FLAGS

UTILITIES COMMUNICATION
HARD DISK INFORMATION
EXIT * The Setup menu contains items for configuring features of the Service Disk
and this configuration is stored on the disk itself. Therefore, do not write
protect this disk.
† Only available when the Service Disk software is installed on a hard

5961291D drive.
PN 4235961E A.6-3
SOFTWARE MENU TREES
A.6-4 PN 4235961E
BDMS DATABASE FIELDS B
B.1 FIELDS CONTAINED IN THE MAXM ANALYZER DATABASE
About the List
The following list show the database fields contained in the MAXM analyzer database. Entries
marked with an asterisk (*) are parameters that are captured by the Backup to Floppy, Service
Parameters Only function. Not all of these parameters are displayed by the Service Disk. Some
of the unmarked (no asterisk) database fields are not being used. These fields may contain
invalid data or may be empty.
MAXM Analyzer 8D Database Fields
Field Name Type Length

0 SAMPLE_MODE_FLAG c 1
1 POSITION_NUMBER c 7
2 POSITION_NUMBER_STATUS c 1
* 3 ID_1 c 17
4 ID_1_STATUS c 1
* 5 JULIAN_DATE 1 4
* 6 ANALYZER_DATE c 9
* 7 TIME_OF_DAY c 9
* 8 RUN_MODE c 1
9 SAMPLE_TICKET_PRINTED_FLAG c 1
10 SAMPLE_PRINTED_FLAG c 1
11 SAMPLE_XMITED_FLAG c 1
12 SAMPLE_SAVED_FLAG c 1
13 SAMPLE_WITH_WL_ENTRY_FLAG c 1
14 PREDILUTE_CBC c 1
15 BARCODE_ENABLED_DISABLED c 1
16 CS_ENABLED_DISABLED c 1
17 ID_LABEL_TYPE c 1
18 I_2_OF_5_LABEL_LENGTH i 2
19 REAGENT_SENSORS_OFF c 1
20 OPR c 4
21 INSTRUMENT_ID c 9
22 UNIT_FORMAT_CODE c 1
23 HGB_BLANK_VOLT c 6
24 HGB_SAMPLE_VOLT c 6
* 25 WBC_1_VAL f 4
* 26 WBC_2_VAL f 4
PN 4235961E B.1-1
DMS DATABASE FIELDSDMS DATABASE FIELDS
FIELDS CONTAINED IN THE MAXM ANALYZER DATABASE

* 27 WBC_3_VAL f 4
* 28 WBC_A_VAL f 4
* 29 RBC_1_VAL f 4
* 30 RBC_2_VAL f 4
* 31 RBC_3_VAL f 4
* 32 RBC_A_VAL f 4
33 HGB_1_VAL f 4
34 HGB_2_VAL f 4
35 HGB_3_VAL f 4
* 36 HGB_A_VAL f 4
37 HCT_1_VAL f 4
38 HCT_2_VAL f 4
39 HCT_3_VAL f 4
* 40 HCT_A_VAL f 4
* 41 MCV_1_VAL f 4
* 42 MCV_2_VAL f 4
* 43 MCV_3_VAL f 4
* 44 MCV_A_VAL f 4
45 MCH_1_VAL f 4
46 MCH_2_VAL f 4
47 MCH_3_VAL f 4
* 48 MCH_A_VAL f 4
49 MCHC_1_VAL f 4
50 MCHC_2_VAL f 4
51 MCHC_3_VAL f 4
* 52 MCHC_A_VAL f 4
53 RDW_1_VAL f 4
54 RDW_2_VAL f 4
55 RDW_3_VAL f 4
* 56 RDW_A_VAL f 4
* 57 PLT_1_VAL f 4
* 58 PLT_2_VAL f 4
* 59 PLT_3_VAL f 4
* 60 PLT_A_VAL f 4
B.1-2 PN 4235961E
FIELDS CONTAINED IN THE MAXM ANALYZER DATABASE B
61 PCT_1_VAL f 4
62 PCT_2_VAL f 4
63 PCT_3_VAL f 4
64 PCT_A_VAL f 4
* 65 MPV_1_VAL f 4
* 66 MPV_2_VAL f 4
* 67 MPV_3_VAL f 4
* 68 MPV_A_VAL f 4
69 PDW_1_VAL f 4
70 PDW_2_VAL f 4
71 PDW_3_VAL f 4
* 72 PDW_A_VAL f 4
* 73 REAL_RETIC_PCT f 4
* 74 RETIC_ABS_VAL f 4
75 REAL_MRV f 4
76 REAL_RMI f 4
* 77 LY_PC_VAL f 4
* 78 MO_PC_VAL f 4
* 79 NE_PC_VAL f 4
* 80 EO_PC_VAL f 4
* 81 BA_PC_VAL f 4
* 82 LY_CNT_VAL f 4
* 83 MO_CNT_VAL f 4
* 84 NE_CNT_VAL f 4
* 85 EO_CNT_VAL f 4
* 86 BA_CNT_VAL f 4
87 TEST_NO c 4
88 WHITE_CYCLES c 1
89 RED_CYCLES c 1
90 PLT_CYCLES c 1
91 VALLEYS f 20
92 X_FLAGS c 8
93 PEAK_CHANNEL i 10
94 DIFF_COUNTS i 10
95 TOTAL_DC_COUNT c 6
PN 4235961E B.1-3

* 96 TOTAL_WHT_COUNT c 6
* 97 DIFF_COMPUTATION_TIME c 6
98 EXPECTED_TIME_DIFF_CELL_CNT c 6
* 99 FLOW_CELL_FULL_CLOG_FLAG c 1
* 100 FLOW_CELL_PARTIAL_CLOG_FLAG c 1
* 101 FLOW_CELL_PARTIAL_2_CLOG_FLAG c 1
102 CELLS_COUNTED_PER_SEC i 40
103 INVALID_DIFF_PERCENTS_FLAG c 1
104 REAL_INMAT_PCT f 4
105 REAL_MSCV f 4
106 TOTAL_RED_COUNT c 6
107 ABD_COUNT c 7
108 RETIC_COMPUTATION_TIME c 6
109 TOTAL_PULSE_COUNT c 6
110 RETIC_VALLEYS f 40
111 RTC_FLAGS c 7
112 DB_REV_LEVEL 1 2
113 SERVICE_FLAG c 1
* 114 DIFF_MODE c 4
* 115 PARTIAL_ASPIRATION c 1
116 INVALID_DATA_FLAG c 1
117 WBC_VOTE_FLAG c 1
118 RBC_VOTE_FLAG c 1
119 HGB_VOTE_FLAG c 1
120 HCT_VOTE_FLAG c 1
121 MCV_VOTE_FLAG c 1
122 MCH_VOTE_FLAG c 1
123 MCHC_VOTE_FLAG c 1
124 RDW_VOTE_FLAG c 1
125 PLT_VOTE_FLAG c 1
126 PCT_VOTE_FLAG c 1
127 MPV_VOTE_FLAG c 1
128 PDW_VOTE_FLAG c 1
129 WBC_OVER_FLAG c 1
B.1-4 PN 4235961E
130 RBC_OVER_FLAG c 1
131 HGB_OVER_FLAG c 1
132 HCT_OVER_FLAG c 1
133 MCV_OVER_FLAG c 1
134 MCH_OVER_FLAG c 1
135 MCHC_OVER_FLAG c 1
136 RDW_OVER_FLAG c 1
137 PLT_OVER_FLAG c 1
138 PCT_OVER_FLAG c 1
139 MPV_OVER_FLAG c 1
140 PDW_OVER_FLAG c 1
141 WBC_INC_FLAG c 1
142 RBC_INC_FLAG c 1
* 143 HGB_INC_FLAG c 1
144 HCT_INC_FLAG c 1
145 MCV_INC_FLAG c 1
146 MCH_INC_FLAG c 1
147 MCHC_INC_FLAG c 1
148 RDW_INC_FLAG c 1
149 PLT_INC_FLAG c 1
150 PCT_INC_FLAG c 1
151 MPV_INC_FLAG c 1
152 PDW_INC_FLAG c 1
153 WBC_A_FLAG c 4
154 RBC_A_FLAG c 4
155 HGB_A_FLAG c 4
156 HCT_A_FLAG c 4
157 MCV_A_FLAG c 4
158 MCH_A_FLAG c 4
159 MCHC_A_FLAG c 4
160 RDW_A_FLAG c 4
161 PLT_A_FLAG c 4
162 PCT_A_FLAG c 4
163 MPV_A_FLAG c 4
164 PDW_A_FLAG c 4
PN 4235961E B.1-5

165 RETIC_PCT_FLAG c 4
166 RETIC_ABS_FLAG c 4
167 MRV_FLAG c 4
168 RMI_FLAG c 4
169 LY_PC_FLAG c 4
170 MO_PC_FLAG c 4
171 NE_PC_FLAG c 4
172 EO_PC_FLAG c 4
173 BA_PC_FLAG c 4
174 LY_CNT_FLAG c 4
175 MO_CNT_FLAG c 4
176 NE_CNT_FLAG c 4
177 EO_CNT_FLAG c 4
178 BA_CNT_FLAG c 4
179 FIT_NOFIT_FLAG c 1
180 ERROR_10_FLAG c 1
181 PHASE_FLAG c 1
182 DATA_TYPE_FLAG c 1
183 RDW_FLAG c 1
184 SA_WBC_HIST_AVG_FLAG c 1
185 SA_RBC_HIST_AVG_FLAG c 1
186 SA_PLT_HIST_AVG_FLAG c 1
187 DISPLAY_WBC_STATUS c 1
188 DISPLAY_WBC_SUSPECT c 1
189 DISPLAY_WBC_DEFINITIVE c 1
190 DISPLAY_RBC_STATUS c 1
191 DISPLAY_RBC_SUSPECT c 1
192 DISPLAY_RBC_DEFINITIVE c 1
193 DISPLAY_PLT_STATUS c 1
194 DISPLAY_PLT_SUSPECT c 1
195 DISPLAY_PLT_DEFINITIVE c 1
196 WBC_ABNORMAL_FLAG c 1
197 RBC_ABNORMAL_FLAG c 1
198 PLT_ABNORMAL_FLAG c 1
B.1-6 PN 4235961E
199 RTC_SAMPLE_FLAGGED c 1
200 SAMPLE_HAS_FLAGS c 1
201 WBC_RAW_AP_1 1 4
204 RBC_RAW_AP_1 1 4
* 207 FLAG c 57
* 208 ID_2 c 17
209 SEQUENCE c 7
210 WORKLIST_STATUS c 1
211 DATE_OF_BIRTH c 11
212 SEX c 1
213 LOCATION c 17
214 PHYSICIAN c 23
215 ENTRY_DATE c 9
216 ENTRY_TIME c 6
217 USER_FIELD1 c 17
220 COMMENT1 c 33
221 COMMENT2 c 33
222 CTRL_SIG_5C c 4
223 OVR_RBC f 4
224 OVR_WBC f 4
225 OVR_PLT f 4
226 OVR_MPV f 4
227 OVR_PDW f 4
228 OVR_MCV f 4
229 OVR_HCT f 4
230 OVR_PCT f 4
231 OVR_RDW f 4
232 OVR_HGB f 4
233 OVR_MCH f 4
PN 4235961E B.1-7

234 OVR_MCHC f 4
* 235 NEDCMN c 1
* 236 NEDCSD i 2
* 237 NERLSMN c 1
* 238 NERLSSD i 2
* 239 NEOPMN c 1
* 240 NEOPSD i 2
* 241 LYDCMN c 1
* 242 LYDCSD i 2
* 243 LYRLSMN c 1
* 244 LYRLSSD i 2
* 245 LYOPMN c 1
* 246 LYOPSD i 2
* 247 MODCMN c 1
* 248 MODCSD i 2
* 249 MORLSMN c 1
* 250 MORLSSD i 2
* 251 MOOPMN c 1
* 252 MOOPSD i 2
253 C1NUM i 2
254 C1DCMN c 1
255 C1DCSD i 2
256 C1RLSMN c 1
257 C1RLSSD i 2
258 C1OPMN c 1
259 C1OPSD i 2
260 C2NUM i 2
261 C2DCMN c 1
262 C2DCSD i 2
263 C2RLSMN c 1
264 C2RLSSD i 2
265 C2OPMN c 1
266 C2OPSD i 2
* 267 PVRATIO i 2
B.1-8 PN 4235961E
* 268 PVRATIO2 i 2
269 RESULTS_CRC i 2
* 270 SAMPLE_ID kd 11
271 ID_1_FLAG kd 18
272 ID_1_DT_FLAG kd 22
273 CP_FLAG kd 8
274 CP_DT_FLAG kd 12
275 ID_1_CP_FLAG kd 25
276 ID_1_CP_DT_FLAG kd 29
277 ID_2_FLAG kd 18
278 ID_2_DT_FLAG kd 22
279 DT_FLAG kd 5
280 FLAGGED kd 5
281 SAVED kd 6
==== 10002 HIST_SCAT
2000 GRAPHICS c 992
2001 HIST_SCAT_CRC i 2
PN 4235961E B.1-9
B.1-10 PN 4235961E
CONTENTS
10
ABBREVIATIONS, ABBREVIATIONS-1
PN 4235961E 10-i
CONTENTS
10-ii PN 4235961E
ABBREVIATIONS
The following list is a composite of the abbreviations, acronyms and reference designators used in this
manual. When the same abbreviation (or reference designator) is used for more than one word (or type of
component), all meanings relevant to this manual are included, separated by semicolons.
SYMBOLS BLK - black

≈ - approximately BRN - brown
°C - degrees Celsius BSV - blood sampling valve
= - equals BT - bath
°F - degrees Fahrenheit Btu - British thermal units
> - greater than BUFF - buffer
> - greater than or equal to
•••• - incomplete computation C
< - less than C - Celsius; circuit; conductivity
< - less than or equal to cal - calibration
µA - micro amperes CBC - complete blood count
µF - microfarad CBT - computer based training
µL - microliter CDRH - Center for Devices and Radiological Health
::::: - parameter code for flow-cell clog CD-ROM - compact disc-read only memory
% - percent CE - Conformitè Europèene, a conformance mark
indicating that the product conforms to any
± - plus/minus applicable European Community directives
I - Ready with regard to product safety and
O - Standby electromagnetic compatibility.
----- - total voteout CFG - configuration
CFR - Code of Federal Regulations
A Ch - channel
A - amperes CHK LBL - check label
ac - alternating current CK - choke
ACLO - ac voltage low CL - cylinder
A/D - analog to digital CLR - clear
adjust. - adjustment cm - centimeters
AMB TEMP - ambient temperature CMPSR - compressor
ANSI - American National Standards Institute CPU - central processing unit
APER - aperture CR - current regulator (semi-conductor device)
ASP - aspiration CRC - cyclic redundancy check
AWG - American wire gauge CTL - control
CURNT - current
B CV -coefficient of variation
B - stepper motor
BA - basophils D
BD - blood/bubble detector d - diameter
PN 4235961E ABBREVIATIONS-1
ABBREVIATIONS
DAC - digital to analog converter FRC - field replaceable components

DC - direct current FRU - field replaceable units
dc - direct current ft - feet
DCN - document control number FU - union fitting
DETECT - detector fxt - fixed
diff - difference; differential FY - Y-fitting
div - division
dL - deciliter G
DLD - download g - gram
DLTR - Diluter GAL - gate array logic
DMA - direct memory access g/dL - grams per deciliter
DMM - digital multimeter GND - ground
DMS - data management system GR - granulocytes
DOS - disk operating system GRN - green
DS - transmitter GRY - grey
DVM - digital voltmeter
DWNLD - download H
Hct - hematocrit
HD - head
E
ed. - edition Hg - mercury
EMC - electromagnetic compatibility Hgb - hemoglobin
EMI - electromagnetic interference Hi - high
EO - eosinophils hsc - hex-socket
EPROM - electronically programmable read only Hz - hertz
memory
EPS - Electronic Power Supply I
ESD - electrostatic discharge IC - integrated circuit
ETL - Electrical Testing Laboratories ID - identifcation
i.d. - internal diameter
F IEC - International Electrical Commission
F - Fahrenheit; fuse in. - inches
FC - flow cell, full clog in. of Hg - inches of mercury
FCC - Federal Communication Commission I/O - input/output
FD - fluid detector INV - invalid
FF - feedthru fitting
FL - air water separator J
fL - femtoliters J - receptacle connector
FM - foam trap
FMT - foam trap
2-ABBREVIATIONS PN 4235961E
ABBREVIATIONS
K MPV - mean platelet volume

K - contactor relay MRV - mean reticulocyte cell volume
kg - kilograms ms - milliseconds
kV - kilovolts MSG RCVD - message received
mV - millivolts
L mVac - millivolts alternating current
laser - light amplification by stimulated emission of
radiation
N
lb - pounds N/A - not applicable
LED - light emitting diode NE - neutrophils
LLS - linear light scatter nm - nanometer
Lo - low ns - nanoseconds
LPS - Laser Power Supply
LS - light scatter O
LTX CAL - latex calibration o.d. - outer diameter
LYSE TRIG -lyse trigger ORN - orange
LV - solenoid OP - opacity
LY - lymphocytes Optns - options
M P
M - dc motor P - platelet; plug connector
m - meter param - parameter
mA - milliamperes PART. - partial
MALS - median angle light scatter PC - partial clog
MB - megabyte Pct - plateletcrit
MC - mixing chamber PDW - platelet distribution width
MCH - mean cell (corpuscular) hemoglobin pg - picograms
MCHC - mean cell (corpuscular) hemoglobin PLB - Peltier module
concentration plt - platelet
MCV - mean cell (corpuscular) volume PM - pump
MF - manifold PN - part number
MFM - modified frequency modulation poly - polyurethane
MHz - mega hertz preamp - preamplifier
MI - maturation index press - pressure
mL - milliliter PROM - programmable read only memory
mm - millimeter ppm - parts per million
MO - monocytes PPS - Pneumatic Power supply
MOD/CMD - mode command PS - power supply
mon -monitor psi - pounds per square inch
PN 4235961E ABBREVIATIONS-3
ABBREVIATIONS
PRTR - Printer TKT PRTR - Ticket Printer

PV - pinch valve TP - test point
TRIG - trigger
Q TTM - Triple Transducer module
Q - receiver (transistor) TX - transmission
QD - quick disconnect
U
R U - opto-isolator switch
R - red; resistor unc - unified coarse thread
RAM - random access memory UL - Underwriters Laboratory
RBC - red blood cell; red blood cell count
RCP - rotary cap-pierce V
RCVD - received V - volts; volume
RDW - red distribution width Vac - vacuum; volts alternating current
reg - regulator VC - vacuum chamber
REPRO - reproducibility VCS - volume, conductivity and scatter
RET - reticulocyte Vdc - volts direct current
retic - reticulocyte VL - valve
RF - radio frequency vltg - voltage
RG - regulator volt - voltage
RIA - red aperture current VREF - reference voltage
RLS - rotated light scatter
RMS - root mean square W
ROM - read only memory W - watt; white
R/W PREAMP - red/white preamplifier w - wide
R/W/P PROC - red/white/platelet processor WBC - white blood cell; white blood cell count
WHT - white
S WIA - white aperture current
S - scatter; second; sensor; switch WLST - worklist
SEC MODE CAL - Secondary-mode calibration WM - wire marker
SF - sweep-flow tank
SLO BLO - slow blow X
ST - sheath tank XMITTED - transmitted
SVP - system verification procedure xrec - cross recess
SW - switch
Y
YEL - yellow
T
TB - terminal board
temp - temperature
4-ABBREVIATIONS PN 4235961E
INDEX
Numerics A
3 CONSECUTIVE FLOWCELL CLOGS, 7.1-1 A/D FAILURE, 7.1-5
3 CONSECUTIVE NO MATCHES, 7.1-1 A/D MEASUREMENT ERROR, 7.1-5
3 CONSECUTIVE NO READS, 7.1-1 abbreviations
3 CONSECUTIVE PARTIAL ASPIRATIONS, 7.1-1 used in this manual, ABBREVIATIONS-1
3 CONSECUTIVE VOTEOUTS, 7.1-2 adjustment procedures
+5 Vdc OUT OF RANGE [XX.XX], 7.1-2 bar-code scanner alignment, Autoloader
+6.3 Vdc OUT OF RANGE [XX.XX], 7.1-2 module, 4.39-1
10 NO READ, NO MATCH, PART.ASP, 7.1-2 blood/bubble detector gains, 4.21-1
10-13 mm TUBE SENSOR ERROR, 7.1-2 BSV alignment, 4.29-9
+12 Vdc OUT OF RANGE [XX.XX], 7.1-3 C1 on the RF Detector Preamp card, 4.11-2
-15 Vdc OUT OF RANGE [XX.XX], 7.1-3 cassette index motor and index hub gap, 4.42-1
+15 Vdc OUT OF RANGE [XX.XX], 7.1-3 CBC lytic reagent and diluent delivery
16 mm TUBE SENSOR ERROR, 7.1-3 timing, 4.20-1
+24 Vdc OUT OF RANGE [XX.XX], 7.1-3 CBC lytic reagent pumps, 4.18-1
30 PSI OUT OF RANGE [XX.XX], 7.1-3 Clog Detector circuit, 4.11-1
30-psi pressure code wheel alignment, 4.31-1
acceptable range, A.1-5 DC, RF, and LS gains, 4.4-2
adjustment procedure, 4.25-3 Electronic Power Supply voltages, 4.23-1
distribution, 2.5-3 Erythrolyse II reagent and StabiLyse reagent
30-psi pressure regulator pumps, 4.16-1
function, A.5-5 Hgb lamp, 4.27-2
location, illustration, A.5-10 laser/flow cell alignment, 4.15-1
60-psi pressure needle-home and needle -forward
acceptable range, A.1-5 sensors, 4.44-1
adjustment procedure, 4.25-1 pressure and vacuum, 4.25-1
adjustment components, new configuration, Primary-mode aspiration pump, 4.17-1
illustration, 4.25-2 Primary-mode calibration factors, 4.7-1
adjustment components, old configuration, RBC and WBC diluent dispensers, 4.19-1
illustration, 4.25-2 rinse block, 4.33-1
distribution, 2.5-2 rocker bed linkage, 4.40-1
60-psi pressure regulator Secondary-mode calibration factors, 4.8-1
location, illustration, 2.5-1 specimen tube stoppers’ pierce proximity, 4.41-1
+240 Vdc OUT OF RANGE [XX.XX], 7.1-3 VCS flow rate (sample and sheath
+300 Vdc OUT OF RANGE [XX.XX], 7.1-4 pressures), 4.10-1
376 CPU card air pump
clearing the RAM, 4.1-3 function, A.5-4
description, 2.4-2 location, illustration, A.5-7
enabling the on-board battery, 3.3-3 ambient temperature sensor
jumper locations, illustration, A.2-1 function, 2.6-4, A.5-1
jumper settings, table, A.2-1 location, 2.6-3
controls Hgb lamp, 2.4-7 location, illustration, A.5-7
inputs/outputs, 2.4-3 Anadex Ticket Printer
location, illustration, 2.4-1 DIP switch settings for, 3.9-1
memory storage on RAM Timer card, 2.4-3 installation procedure, 3.9-1
receives pulses from VCS PROCESSOR Printer Logic Control card. See Printer Logic
card, 2.4-9 Control card
role in histogram development, 2.4-10 self-test procedure, 3.9-1
software download sequence, 2.4-2 self-test switch, location, 3.9-1
+1350 Vdc OUT OF RANGE [XX.XX], 7.1-4 ANALYSIS NOT DONE, 7.1-5
PN 4235961E INDEX-1
INDEX
Analyzer module component part numbers, master parts

card cage with circuit cards inserted, list, 8.1-2
illustration, 2.4-1 description, 2.9-1
component part numbers, illustrated parts ensuring Sample Handler card is configured
list, 8.2-47 for, 2.7-4
description, 2.4-1 function, 2.9-1
exhaust fan location, 3.1-2 location, illustration, 2.1-2
function, 2.4-1 main component descriptions, table of, 2.9-1
location, illustration, 2.1-2 main component locations, front view, rocker
ANALYZER POWER INTERRUPTION, 7.1-5 bed backward, 2.9-4
Analyzer/Systems Control module. See Analyzer main component locations, front view, rocker
module bed forward, 2.9-3
Aperture module and bath assembly main component locations, rear view, 2.9-4
exploded view, 4.28-1 needle replacement procedure, 4.32-1
preventing damage to the O-rings, 4.28-2 operation, summary, 2.9-5
replacement, 4.28-1 preventing damage to during software
See also RBC bath; WBC bath download, 2.7-4
aperture viewing screens removal procedure, 4.34-1
function, A.5-1 retaining brackets location, illustration, 3.2-5
location, illustration, A.5-8 Rocker bed Interface card. See Rocker Bed
Aperture Voltage/Power Fail Detect card Interface card
location, illustration, 2.2-3 rocker bed. See rocker bed
aspiration pump, Primary mode sensor and switch functions and location
function, A.5-4 references, table of, 4.43-1
location, illustration, A.5-7, A.5-11, A.5-12 shipping screw location, illustration, 3.2-5
volume adjustment procedure, 4.17-1 shipping screw, removal, 3.2-5
volume tolerances, A.1-10 AUTOLOADER PAUSED, 7.1-5
aspiration pump, Secondary mode Autosensor Test card
function, A.5-4 function, 2.10-1
location, illustration, A.5-7, A.5-11, A.5-12 LEDs lit following power up, illustration, 2.10-1
aspirator tip
function, A.5-1
location, illustration, A.5-7 B
ATTENTION B1. See carousel motor; load elevator motor
heading defined, 1.1-3 B2. See unload elevator motor
Autoloader Interface card BA%
block diagram, 2.7-2 verifying reproducibility in the Primary
component locations, illustration, A.2-2 mode, 4.5-1
connectors, table, A.2-2 verifying reproducibility in the Secondary
function, 2.9-7 mode, 4.5-2
inputs/outputs, 2.9-8 BA% and BA#
location, illustration, 2.9-4 part of reported WBC differential, 2.1-1
Autoloader module See also BA%; diff parameters
Autoloader Interface card. See Autoloader background count
Interface card diluent, acceptable limits, A.1-2
block diagram of the associated circuit LATRON primer, acceptable limits, A.1-8
cards, 2.7-2 backwash
component part numbers, illustrated parts description, 2.11-10
list, 8.2-29, 8.2-31, 8.2-33, 8.2-35 BACKWASH NOT PERFORMED, 7.1-5
2-INDEX PN 4235961E
INDEX
backwash pump bed-lock cylinder

function, A.5-4 adjusting to achieve optimal vertical
location, illustration, A.5-10 tube-piercing position, 4.41-2
backwash safety sensor function, 2.9-2
action to change state, Autoloader location, illustration, 2.9-4
module, 4.43-1 biological hazards, 1.2-3
action to change state, Rotary Cap-Pierce contamination, prevention of, 1.2-4
module, 4.43-5 personal injury, prevention of, 1.2-3
function and location reference, Autoloader publications on decontamination, 1.2-4
module, 4.43-1 WARNING for customers, 1.2-4
function and location reference, Rotary bleach chamber
Cap-Pierce module, 4.43-5 function, A.5-6
BAD DOWNLOAD MSG RCVD - 196 CODE, 7.1-5 location, illustration, A.5-10
BAD PORT IN USE TO SEND DATA, 7.1-5 bleach probe
bar-code detector sensor function, A.5-1
action to change state, 4.43-6 location, illustration, A.5-7
function and location reference, 4.43-6 block diagrams
function, type, and location reference, 2.8-2 DILUTER INTERFACE card and Solenoid
location, illustration, 2.8-2 Junction card, 2.4-5
BARCODE NOT READ, 7.1-5 electronic signal flow, MAXM analyzer with
bar-code reader Autoloader module, 2.3-2
function, 2.8-1, 2.9-1 electronic signal flow, MAXM analyzer with
location, illustration, 2.8-2, 2.9-3 Rotary Cap-Pierce module, 2.3-1
location, illustration, Autoloader module, 2.9-4 I/O card, 2.4-6
not reading cassette and tube bar-code LS Preamp 5 module, 2.13-7
labels, 7.5-1 Peltier Controller card, 2.6-3
Bar-Code Reader Decoder card Pneumatic Monitor card, 2.5-4
code type default settings, table, A.4-1 R/W/P PROC card and R/W PREAMP
communication default settings, table, A.4-1 card, 2.4-11
configuration procedure, 4.38-1 Sample Handler card with Autoloader
configuring at instrument installation, 3.4-1 module, 2.7-2
operations default settings, table, A.4-2 Sample Handler card with Rotary Cap-Pierce
user outputs default settings, table, A.4-2 module, 2.7-3
BARCODE READER DID NOT RESPOND, 7.1-5 VCS PROCESSOR card, 2.4-8
bar-code scanner BLOOD COMPARISON OUT OF LIMITS, 7.1-6
alignment procedure, Autoloader blood sampling valve. See BSV
module, 4.39-1 blood/bubble detectors
bar-code system, part numbers, 8.1-4 acceptable cable voltage readings, A.1-9
bar-code wand adjusting gains at instrument installation, 3.5-2
cable connections, 3.3-2 adjustments and test points, location,
cable connections without a keyboard adapter, illustration, A.2-12, A.2-14
illustration, 3.3-2 checks made during aspiration, 2.11-3
BARCODE WAND NO READ, 7.1-6 function, 2.11-3
basophils. See BA gain adjustment procedure, 4.21-1
baths. See Aperture module and bath assembly; RBC location, illustration, A.5-7
bath; WBC bath replace with a matched set, 4.21-2
BD1. See blood/bubble detectors boards, circuit. See cards, circuit
BD2. See blood/bubble detectors BSV
bed position sensors. See horizontal position sensor; alignment procedure, 4.29-9
pierce position sensor and housing verification procedure, 4.29-11
PN 4235961E INDEX-3
INDEX
avoiding physical damage to when CAL FACTORS NOT WITHIN LIMITS, 7.1-7
disassembling, 4.29-2, 4.29-3 calibration, CBC
description, 2.11-2 assisting the customer with S-CAL calibration at
disassembly, 4.29-1 instrument installation, 3.7-4
flow paths and wire markers, illustration, 2.11-3 determining Secondary-mode calibration
function in Primary mode, 2.11-4 factors, 4.8-1
function in Secondary mode, 2.11-7 initial adjustment of Primary-mode calibration
housing replacement, 4.29-5 factors, 4.7-1
location, illustration, A.5-7 latex calibration procedure, 4.4-1
ports and wire markers, illustration, 2.11-3 making initial adjustments at instrument
replace sections as a matched set, 4.29-1 installation, 3.7-3
replacement, 4.29-1 tolerances and limits, A.1-1
shipping spacers, removal, 3.2-4 verifying latex calibration at instrument
See also BSV actuator installation, 3.7-1
BSV actuator calibration, VCS
assembly, exploded view, 4.29-7 diff latex calibration procedure, 4.4-2
function, A.5-1 retic latex calibration procedure, 4.4-2
location, illustration, A.5-7 tolerances and limits, A.1-8
replacement, 4.29-6 troubleshooting diff or retic latex calibration
BSV module problems, 7.4-1
component locations, A.5-7, A.5-8 verifying diff latex calibration at instrument
component part numbers, illustrated parts installation, 3.7-2
list, 8.2-13, 8.2-15, 8.2-17, 8.2-19 verifying retic latex calibration at instrument
component part numbers, master parts installation, 3.7-2
list, 8.1-5 See also VCS optimization
location, illustration, 2.1-2 CANNOT BATCH. SYSTEM IN RUN MODE, 7.1-7
BT1 (WBC bath) CANNOT CYCLE WHILE BATCH
function, A.5-2 PROCESSING, 7.1-7
location, illustration, A.5-8 CANNOT MOVE CASSETTE ON BED, 7.1-7
See also WBC bath CANNOT OPEN RAW.DAT FILE, 7.1-7
BT2 (RBC bath) CANNOT ROCK BED, 7.1-7
function, A.5-2 CANNOT STORE RET RESULTS IN 5C FILE, 7.1-8
location, illustration, A.5-8 CANNOT TRANSMIT CAL FACTORS, 7.1-8
See also RBC bath cards, circuit
bubble trap 376 CPU. See 376 CPU card
function, A.5-6 Aperture Voltage/Power Fail Detect. See
location, illustration, A.5-8 Aperture Voltage/Power Fail Detect card
bubble/blood detectors. See blood/bubble detectors Bar-Code Reader Decoder. See Bar-Code Reader
Decoder card
DILUTER INTERFACE. See DILUTER
C INTERFACE card
C (conductivity or RF) Electronic Power Supply Terminal. See
troubleshooting latex calibration Electronic Power Terminal card
problems, 7.4-1 Fluid Detector/Ram Pressure. See Fluid
VCS measurement, description, 2.13-1 Detector/Ram Pressure card
cables, electronic I/O. See I/O card
bar-code wand connections, 3.3-2 part numbers, 8.1-8
bar-code wand connections, illustration, 3.3-2 Peltier Controller. See Peltier Controller card
instrument, connections, 3.3-1 Pneumatic Monitor. See Pneumatic Monitor card
instrument, connections, illustration, 3.3-1
4-INDEX PN 4235961E
INDEX
Pneumatic Power Supply Buck-Boost cassette position 1 sensor

Transformer Terminal. See Pneumatic Power action to change state, 4.43-4
Supply Buck-Boost Transformer Terminal function and location reference, 4.43-4
card function, type, and location reference, 2.9-2
Printer Logic Control. See Printer Logic Control location, illustration, 2.9-3
card cassette position 2 sensor
R/W PREAMP. See R/W PREAMP card action to change state, 4.43-4
R/W/P PROC. See R/W/P PROC Card function and location reference, 4.43-4
RAM Timer. See RAM timer card function, type, and location reference, 2.9-2
RF Power Supply. See RF Power Supply card location, illustration, 2.9-3
Sample Handler. See Sample Handler card cassette position 3 sensor
VCS PROCESSOR. See VCS PROCESSOR card action to change state, 4.43-4
carousel assembly function and location reference, 4.43-4
code wheel alignment procedure, 4.31-1 function, type, and location reference, 2.9-3
code wheel detail, illustration, 4.31-2 location, illustration, 2.9-3
component part numbers, 8.2-27 CASSETTE UNLOAD FAILURE, 7.1-9
function, 2.8-1 CAUTION
location, illustration, 2.8-2 heading defined, 1.1-3
motor replacement, 4.30-1 CBC
CAROUSEL DID NOT ROTATE, 7.1-8 noise checks, 4.12-1
carousel motor CBC CALIBRATION TABLE FULL, 7.1-9
function, type, and location reference, 2.8-1 CBC calibration. See calibration, CBC
location, illustration, 2.8-2 CBC DATA ACQUISITION FAILURE, 7.1-9
carryover checks CBC lyse sensor
acceptable limits, A.1-2 action to change state, 4.43-2
procedure, 4.6-1 function, 4.43-2
CARRYOVER IS ACTIVE, 7.1-8 See also sensors, level
carryover, Primary mode CBC lytic reagent
acceptable limits, A.1-2 and diluent delivery timing check, 4.20-1
checking, procedure, 4.6-1 and diluent delivery timing check digital
verifying at instrument installation, 3.7-2 pressure meter hookup, illustration, 4.20-3
carryover, Retic mode and diluent delivery timing check test box and
acceptable limits, A.1-2 transducer hookup, illustration, 4.20-2
checking, procedure, 4.6-2 and diluent delivery timing tolerances, A.1-7
verifying at instrument installation, 3.7-3 and diluent timing, verifying at instrument
carryover, Secondary mode installation, 3.7-1
acceptable limits, A.1-2 choke configurations for changing delivery
checking, procedure, 4.6-2 timing, A.1-7
verifying at instrument installation, 3.7-2 function, 2.6-1
cassette index motor instrument setup procedure, 3.5-1
and index hub gap adjustment, 4.42-1 level sensing, 2.6-1
function, type, and location reference, 2.9-2 pumps. See CBC lytic reagent pumps
location, illustration, 2.9-4 sensor (FD5). See CBC lyse sensor
CASSETTE LABEL NO READ, 7.1-8 tubing connection to instrument,
CASSETTE LOAD FAILURE, 7.1-8 illustration, 3.3-4
cassette position 0 sensor CBC lytic reagent pumps
action to change state, 4.43-4 function, A.5-4
function and location reference, 4.43-4 location, illustration, A.5-8
function, type, and location reference, 2.9-3 volume adjustment procedure, 4.18-1
location, illustration, 2.9-3 volume tolerances, A.1-10
PN 4235961E INDEX-5
INDEX
CBC module clamp cylinder

component locations, A.5-8, A.5-9 location, illustration, 2.8-2
component part numbers, illustrated parts CLEANER OUT, 7.1-9
list, 8.2-3, 8.2-5 cleaner sensor
component part numbers, master parts action to change state, Autoloader
list, 8.1-7 module, 4.43-1
location, illustration, 2.1-2 action to change state, Rotary Cap-Pierce
CBC parameters module, 4.43-5
reported by instrument, 2.1-1 function and location reference, Autoloader
See also CBC technology module, 4.43-1
CBC technology function and location reference, Rotary
applying the Coulter principle, 2.12-1 Cap-Pierce module, 4.43-5
determining the Hgb, 2.12-2 See also sensors, level
MCV, RDW, Plt and MPV voting criteria, 2.4-13 cleaning agent
parameters reported, 2.1-1, 2.12-1 container, neck support bracket installed, 3.3-5
RBC and WBC counting sequences, 2.4-10 function, 2.6-1
RBC and WBC voting criteria, 2.4-10 instrument setup procedure, 3.5-1
RBC, WBC, and Plt histogram level sensing, 2.6-1
development, 2.4-10 sensor (FD7). See cleaner sensor
RBC, WBC, and Plt histogram particle tubing connection to instrument,
accumulation, 2.4-10 illustration, 3.3-4
See also calibration, CBC cleaning agent pump
circuit cards. See cards, circuit function, A.5-5
circuits location, illustration, A.5-10
Clog Detector adjustment, 4.11-1 cleaning procedures
CL1 flow cell, 4.13-1
function, A.5-2 lens block, 4.14-1
location, illustration, A.5-7 Clog Detector
CL2 adjusting circuit at instrument installation, 3.7-1
function, A.5-2 error generated by, 7.2-1
location, illustration, A.5-7 Clog Detector circuit adjustment, 4.11-1
CL3 COMMAND COMPLETION NOT
function, A.5-2 SUCCESSFUL, 7.1-9
location, illustration, A.5-8 COMMAND TO DIGIBOARD NOT
CL4 ACCEPTED, 7.1-9
location, illustration, 2.8-2 compressor
CL5 monitoring bleed sequence, 2.5-5
location, illustration, 2.8-2 shipping pins location, illustration, 3.2-3
CL6 COMPRESSOR DID NOT BLEED [XX.XX], 7.1-9
location, illustration, 2.9-4 COMPRESSOR PRESSURE ERROR [XX.XX], 7.1-10
See also needle-drive cylinder conductivity (C or RF)
CL7 VCS measurement, description, 2.13-1
location, illustration, 2.9-4 connections, tubing
CL8 neck support bracket, 3.3-5
location, illustration, 2.9-4 reagent input, illustration, 3.3-4
See also bed-lock cylinder control files
CL9 setting up at instrument installation, 3.7-4
location, illustration, 2.9-4 setup procedure, reference, 3.7-4
CONTROL SAMPLE IDENTIFIED, 7.1-10
6-INDEX PN 4235961E
INDEX
COULD NOT ACCESS AUTOPRINT QUEUE, 7.1-10 Data Management System. See DMS
COULD NOT ACCESS BATCH QUEUE, 7.1-10 database
COULD NOT ACCESS MANUAL QUEUE, 7.1-10 fields, B.1-1
COULD NOT CLOSE AUTOPRINT QUEUE, 7.1-10 DC (V or volume)
COULD NOT CLOSE BATCH QUEUE, 7.1-10 troubleshooting latex calibration
COULD NOT CLOSE MANUAL QUEUE, 7.1-10 problems, 7.4-1
COULD NOT DELETE AUTOPRINT DATA, 7.1-10 VCS measurement, description, 2.13-1
COULD NOT DELETE AUTOPRINT QUEUE, 7.1-11 diagrams, block. See block diagrams
COULD NOT DELETE BATCH DATA, 7.1-11 DIFF DATA ACQUISITION FAILURE, 7.1-13
COULD NOT DELETE BATCH QUEUE, 7.1-11 diff latex calibration. See calibration, VCS
COULD NOT DELETE MANUAL DATA, 7.1-11 diff leukocyte preservative
COULD NOT DELETE MANUAL QUEUE, 7.1-11 function, 2.6-1
COULD NOT FIND AUTOPRINT DATA, 7.1-11 level sensing, 2.6-1
COULD NOT FIND BATCH DATA, 7.1-11 See also StabiLyse reagent
COULD NOT FIND MANUAL DATA, 7.1-11 diff lytic reagent
COULD NOT OPEN AUTOPRINT QUEUE, 7.1-11 function, 2.6-1
COULD NOT OPEN BATCH QUEUE, 7.1-11 level sensing, 2.6-1
COULD NOT OPEN MANUAL QUEUE, 7.1-12 See also Erythrolyse II reagent
COULD NOT READ FROM AUTOPRINT diff mixing chamber
QUEUE, 7.1-12 function, A.5-3
COULD NOT READ FROM BATCH QUEUE, 7.1-12 location, illustration, A.5-10
COULD NOT READ FROM MANUAL diff parameter
QUEUE, 7.1-12 noise checks, 4.12-1
COULD NOT WRITE TO AUTOPRINT diff parameters
QUEUE, 7.1-12 method used for determining, 2.13-1
COULD NOT WRITE TO BATCH QUEUE, 7.1-12 reported by instrument, 2.1-1
COULD NOT WRITE TO MANUAL QUEUE, 7.1-12 See also VCS technology
Coulter principle diff pressure
applying, 2.12-1 acceptable range, A.1-5
sensing area, illustration, 2.12-1 monitoring, 2.5-4
COULTER® MAXM Instrument Reference Form, DIFF PRESSURE OUT OF RANGE, 7.1-13
part number, 5.1-1 diff waste chamber
COULTER® SYSTEM VERIFICATION FORM, part function, A.5-6
number, 5.1-1 location, illustration, A.5-10
counting sequences, RBC and WBC, 2.4-10 diluent
covers, instrument and CBC lytic reagent delivery timing
part numbers, 8.1-9 check, 4.20-1
CRC ERROR ON READ SYSTEM.CFG FILE, 7.1-12 and CBC lytic reagent delivery timing check
CTL FILE I/O ERROR, 7.1-12 digital pressure meter hookup,
CTL FILE NN, <FILE> IS FULL, 7.1-12 illustration, 4.20-3
customer documents. See manuals, customer and CBC lytic reagent delivery timing check test
cylinders box and transducer hookup,
bed lock. See bed-lock cylinder illustration, 4.20-2
needle drive. See needle-drive cylinder and CBC lytic reagent delivery timing
tolerances, A.1-7
and lytic reagent timing, verifying at instrument
D installation, 3.7-1
data accumulation choke configurations for changing delivery
for RBC and WBC counts, 2.4-10 timing, A.1-7
for RBC, WBC and Plt histograms, 2.4-10 conditions causing a PC2 error, 7.2-2
PN 4235961E INDEX-7
INDEX
container, neck support bracket installed, 3.3-5 DILUTER TABLE ERROR, 7.1-13
dispensers. See RBC diluent dispenser; WBC DISK DRIVE C: COULD NOT BE ACCESSED, 7.1-13
diluent dispenser DISK DRIVE C: IS FULL, 7.1-14
function, 2.6-1 DISK DRIVE D: IS FULL, 7.1-14
instrument setup procedure, 3.5-1 DISK FULL - ARCHIVING DISCONTINUED, 7.1-14
level sensing, 2.6-1 dispensers, diluent. See RBC diluent dispenser;
sensor (FD6). See diluent sensor WBC diluent dispenser
temperature control for mixing chamber DMS
rinse, 2.6-4 cable connections, illustration, 3.3-1
tubing connection to instrument, fan location, 3.1-2
illustration, 3.3-4 leakage current specifications, 2.1-5
DILUENT COMPARISON OUT OF LIMITS, 7.1-13 line voltage select switch. See DMS line voltage
DILUENT OUT, 7.1-13 select switch
diluent sensor location requirements, 3.2-1
action to change state, Autoloader service options available, 4.2-2
module, 4.43-1 software download sequence, 2.4-2
action to change state, Rotary Cap-Pierce testing at instrument installation, 3.4-1
module, 4.43-5 DMS Configuration Listing for STKS and MAXM
function and location reference, Autoloader Series Systems
module, 4.43-1 part number, 1.1-1
function and location reference, Rotary DMS line voltage select switch
Cap-Pierce module, 4.43-5 caution when setting, 3.3-3
See also sensors, level location, illustration, 3.3-3
Diluter setting at instrument installation, 3.3-3
backwash description, 2.11-10 DMS TIMEOUT, 7.1-14
color coding of tubing, 2.11-2 documents
description, 2.11-1 manuals. See manual; manuals, customer
fluidic components, table of descriptions and safety. See documents, safety
location references, A.5-1 service forms, provided in manual, 5.1-1
function, 2.11-1 documents, safety
function of blood/bubble detectors, 2.11-3 ANSI standard 136.1, SAFE USE OF LASERS,
function of BSV, 2.11-2 ordering information, 1.2-1
function of solenoid valves, 2.11-1 biological decontamination references, 1.2-4
hardware, miscellaneous, part numbers, 8.1-12 laser radiation regulations, 1.2-2
location, illustration, 2.1-2 door interlock
main components, 2.11-1 action to change state, Autoloader module
modules that can be repositioned for better (S5), 4.43-2
access, 2.11-1 action to change state, Rotary Cap-Pierce
sample processing description, 2.11-4 module (S18), 4.43-6
DILUTER INTERFACE card function and location reference, Autoloader
block diagram, 2.4-5 module (S5), 4.43-2
component locations, illustration, A.2-3 function and location reference, Rotary
controls solenoids in the Diluter, 2.11-1 Cap-Pierce module (S18), 4.43-6
function, 2.4-4 function, type, and location reference,
inputs/outputs, 2.4-4 Autoloader module (S5), 2.9-2
jumper setting, A.2-3 function, type, and location reference, Rotary
location, illustration, 2.4-1 Cap-Pierce module (S18), 2.8-2
lyse trigger test point, location/function, 2.4-4 location, illustration, Autoloader module
test point, A.2-3 (S5), 2.9-3
8-INDEX PN 4235961E
INDEX
location, illustration, Rotary Cap-Pierce module connectors and adjustments, illustration, 4.23-2
(S18), 2.8-2 connectors and test points, illustration, 4.23-2
doors, instrument connectors, front and top, illustration, 2.2-2
part numbers, 8.1-9 connectors, illustration, 4.22-1
DOWNLOAD NOT SUCCESSFUL, 7.1-14 description, 2.2-1
drain line exhaust fan location, 3.1-2
tubing connection to instrument, function, 2.2-2
illustration, 3.3-4 fuses, part numbers, 8.1-9
DS2 (hand detector sensor). See hand detector input power switch settings for ac line input,
sensor table, A.3-1
DS2 (bar-code detector sensor). See bar-code location, illustration, 2.1-2, 2.1-3
detector sensor main components, 2.2-1
main components, front view, illustration, 2.2-2
main components, top view, illustration, 2.2-3
E power cable, length specification, 3.1-1
electronic hazards, 1.2-3 replacement procedure, 4.22-1
instrument damage, prevention of, 1.2-3 Terminal card jumpers and connections,
personal injury, prevention of, 1.2-3, 2.2-3 table, A.3-2
electronic interference test points and adjustments, table, A.3-2
on the ac input line, acceptable limits, A.1-5 testing at instrument installation, 3.4-1
preventing, 3.3-1, 3.3-4 voltages adjustment procedure, 4.23-1
See also electronic noise Electronic Power Supply Terminal card
electronic noise configuring after Electronic Power Supply
checking at instrument installation, 3.7-1 replacement, 4.22-1, 4.23-1
checking levels on the RBC, WBC, and Plt configuring at instrument installation, 3.2-4
channels, 4.12-1 jumpers and connections, table, A.3-2
checking levels, procedure, 4.12-1 electronic service manuals, obtaining, 1.1-1
checking levels, troubleshooting Electronic system
procedure, 7.3-1 Analyzer module. See Analyzer module
checking RMS noise levels on the DC, RF, and LS cable connections, 3.3-1
channels, 4.12-1 cable connections, illustration, 3.3-1
RBC, WBC, Plt test points for color coding of wires, 2.2-3
measurement, 2.4-13 Electronic Power Supply. See Electronic Power
tolerances, CBC, A.1-2 Supply
tolerances, diff and retic, A.1-8 input requirements, 3.1-1
troubleshooting problems on the RF overview, 2.2-1
channel, 7.3-3 precautions for preventing electronic
VCS test points for measurement, 2.4-9 noise, 3.3-1, 3.3-4
See also electronic interference signal flow, MAXM analyzer with Autoloader
Electronic Power Supply module, 2.3-2
caution required when installing, 3.2-4, 4.22-2 signal flow, MAXM analyzer with Rotary
component part numbers, illustrated parts Cap-Pierce module, 2.3-1
list, 8.2-43 voltage adjustments, 4.23-1
configuration criteria for ac line input, voltage monitoring, 2.2-4
table, A.3-2 elevator. See load elevator; unload elevator
configuring Electronic Power Supply Terminal engineering schematics
card after replacing Electronic Power available but not included in manual, list
Supply, 4.22-1, 4.23-1 off, 6.1-1
configuring Electronic Power Supply Terminal included in manual, list of, 6.1-1
card at instrument installation, 3.2-4
PN 4235961E INDEX-9
INDEX
EO% FD3 (Erythrolyse reagent sensor)

verifying reproducibility in the Primary function, A.5-2
mode, 4.5-1 location, illustration, A.5-10
verifying reproducibility in the Secondary See also Erythrolyse (reagent) sensor
mode, 4.5-2 FD4 (Stabilyse reagent sensor)
EO% and EO# function, A.5-2
part of reported WBC differential, 2.1-1 location, illustration, A.5-10
See also diff parameters See also Stabilyse (reagent) sensor
eosinophils. See EO FD5 (CBC lyse sensor)
ERROR FILE I/O ERROR, 7.1-14 function, A.5-2
error messages location, illustration, A.5-10
tables of, 7.1-1 See also CBC lyse sensor
See also individual messages FD6 (diluent sensor)
ERROR READING SYSTEM.CFG FILE, 7.1-14 function, A.5-2
ERROR UPDATING SYSTEM.CFG FILE, 7.1-14 location, illustration, A.5-10
Erythrolyse (reagent) sensor See also diluent sensor
action to change state, 4.43-2 FD7 (cleaner sensor)
function and location reference, 4.43-2 function, A.5-3
See also sensors, level location, illustration, A.5-10
Erythrolyse II reagent See also cleaner sensor
conditions causing a PC2 error, 7.2-2 FILE I/O ERROR, 7.1-14
instrument setup procedure, 3.5-1 FL1
pumps. See Erythrolyse II reagent pumps function, A.5-3
sensor (FD3). See Erythrolyse (reagent) sensor location, illustration, A.5-10
temperature control, 2.6-4 FL2
tubing connection to instrument, function, A.5-3
illustration, 3.3-4 location, illustration, A.5-10
See also diff lytic reagent flow cell
Erythrolyse II reagent pumps and laser alignment procedure, 4.15-1
function, A.5-4 and laser alignment procedure problems,
location, illustration, A.5-11, A.5-12 troubleshooting, 4.15-1
replacement procedure, 4.16-8 and laser alignment work flow,
volume adjustment procedure, 4.16-1 illustration, 4.15-1
volume optimization work flow, cleaning procedure, 4.13-1
illustration, 4.16-3 errors, troubleshooting, 7.2-1
EXTENSIVE FLAGS GENERATED, 7.1-14 location, illustration, 2.13-3
ports and sample and reagent flow,
illustration, 2.13-4
F Flow-Cell module
FC, 7.2-1 location, illustration, 2.1-3
FC1 (flow cell). See flow cell See also TTM
FD1 (RBC bath overflow detector) Fluid Detector/Ram Pressure card
function, A.5-2 component locations, illustration, A.2-4
location, illustration, A.5-9 connectors, table, A.2-4
FD2 (WBC bath overflow detector) function, 2.6-1
function, A.5-2 LED functions, table, A.2-4
location, illustration, A.5-9 LED locations/functions, 2.6-2
location, illustration, 2.1-3, A.5-10
10-INDEX PN 4235961E
INDEX
FMT1 checking carryover in the Primary mode, 4.6-1

function, A.5-3 lamp. See Hgb lamp
location, illustration, A.5-11, A.5-12 method used for determining, 2.12-2
FMT2 Preamp module. See Hgb Preamp module
function, A.5-3 regulator location, 2.4-7
location, illustration, A.5-10 verifying reproducibility in the Primary
FMT3 mode, 4.5-1
function, A.5-3 verifying reproducibility in the Secondary
location, illustration, A.5-9 mode, 4.5-2
FMT4 Hgb lamp
function, A.5-3 adjustment procedure, 4.27-2
location, illustration, A.5-7 control, 2.4-7
forms function, 2.12-2, A.5-1
Notice of Information Update, when used, 1.1-1 location, illustration, A.5-8
forms, service, provided in manual, 5.1-1 HGB OUT OF RANGE, 7.1-15
full cass index rotation sensor Hgb Preamp module
action to change state, 4.43-3 function, A.5-1
function and location reference, 4.43-3 location, illustration, A.5-8
function, type, and location reference, 2.9-2 preventing electronic damage to, 4.27-1
location, illustration, 2.9-3 replacement procedure, 4.27-1
fuses high vacuum
panel location, illustration, 2.1-4 acceptable range, A.1-5
part numbers, 8.1-9 distribution, 2.5-3
ratings and functions, table, A.3-1 verification, 4.25-5
HIGH VACUUM OUT OF RANGE, 7.1-15
histograms
G particle accumulation, 2.4-10
Graphic Printer. See Printer, Graphic RBC, WBC, and Plt development, 2.4-10
guidelines horizontal position sensor
for servicing the instrument, 4.1-1 action to change state, 4.43-3
function and location reference, 4.43-3
H function, type, and location reference, 2.9-3
location, illustration, 2.9-4
hand detector sensor
HOST COMM. PARAMETERS UNDEFINED, 7.1-15
action to change state, 4.43-6
HOST TX BUFF REQUEST NOT
SUCCESSFUL, 7.1-15
function, type, and location reference, 2.8-1
humidity and temperature
requirements, 3.1-2
hardware, miscellaneous
part numbers, 8.1-12
hazards I
biological, 1.2-3 I/O card
electronic, 1.2-3 block diagram, 2.4-6
laser beam, 1.2-1 converts analog Hgb reading to digital
laser radiation, 1.2-2 value, 2.12-2
hematocrit. See Hct function, 2.4-6
hemoglobin. See Hgb inputs/outputs, 2.4-7
Hgb issues ZAP COMMAND to R/W PREAMP
acceptable blank voltage, A.1-9 card, 2.4-14
adjusting blank at instrument installation, 3.5-1 jumper locations, illustration, A.2-5
PN 4235961E INDEX-11
INDEX
jumper settings, A.2-5 electronic cable connections, 3.3-1

location, illustration, 2.4-1 electronic cable connections, illustration, 3.3-1
monitors LS offset voltage, 2.13-8 entering institution information, 3.5-1
monitors voltage for Electronic system, 2.2-4 function, 2.1-1
monitors waste float sensor, 2.6-5 guidelines for servicing, 4.1-1
ID CANCELED-TIME EXPIRED, 7.1-16 installation. See installation, instrument;
IDENTIFICATION REQUIRED, 7.1-16 installation procedures, instrument
ILLEGAL INSTRUMENT REPLY, 7.1-16 leakage current specifications, 2.1-5
IMPORTANT lockups, 7.5-1
heading defined, 1.1-3 main components, 2.1-1
INCOMPATIBLE SAMPLE HANDLER maintenance and verification procedures
SOFTWARE, 7.1-16 recommended, 5.1-1
INCOMPLETE NEEDLE EXTRACT, 7.1-16 modes of operation, 2.1-5
INCOMPLETE NEEDLE PIERCE, 7.1-16 parameters reported, 2.1-1
INCOMPLETE NEEDLE RETRACT, 7.1-17 performance specifications and characteristics
INCOMPLETE RAW DATA TRANSMISSION, 7.1-17 reference, 2.1-4
INCOMPLETE TUBE FORWARD, 7.1-17 physical specifications reference, 2.1-4
INCOMPLETE TUBE RETRACT, 7.1-17 powering down, 4.1-2
INCONSISTENT SAMPLE HANDLER powering up, 4.1-2
HARDWARE, 7.1-17 reagent and drain connections, 3.3-4
installation procedures, instrument reagent and drain connections,
account/instrument information forms for, 3.8-1 illustration, 3.3-4
adjustments and calibration, 3.7-1 resetting, 4.1-2
checklist, 3.8-1 service forms provided in manual, 5.1-1
connecting assemblies, 3.3-1 software download sequence, 2.4-2
initial setup, 3.2-1 software menu trees. See software menu trees
initial system setup, 3.5-1 space and accessibility requirements, 3.1-1
preinstallation checks, 3.1-1 specifications. See specifications
system testing, 3.6-1 supplies required at installation, 3.1-3
testing/configuring assemblies, 3.4-1 systems, list of, 2.1-4
installation procedures, options and upgrades temperature and humidity requirements, 3.1-2
Anadex Printer, 3.9-1 ventilation requirements, 3.1-2
Graphic Printer, reference, 3.9-1 verification procedure, 5.1-1
installation, instrument whole blood verification procedure, 4.3-1
account information form, example for INSTRUMENT CONFIGURATION ERROR, 7.1-17
printing, 3.8-1 INSTRUMENT INTERNAL ERROR XXX, 7.1-18
data log sheets, examples for printing, 3.8-2 INSTRUMENT REPLY TIMEOUT, 7.1-18
DMS location requirements, 3.2-1 INSTRUMENT TO 196 CODE DWNLD
main component layout, illustration, 3.2-1 FAILED, 7.1-18
supplies required, 3.1-3 Instrument Verification Form. See system
use of Installation Report Form, 3.2-5 verification procedure
installation, software interconnect cables
reference, 3.4-1 part numbers, 8.1-10
institution information, setting up, 3.5-1 interference, electronic. See electronic interference
instrument INV LABEL - STORED IN CURRENT MODE, 7.1-18
database fields, B.1-1 INVALID 376 MOD/CMD RCVD 196
diagnostic products reference, 2.1-5 DWNLD, 7.1-18
drainage requirements, 3.1-3 INVALID 376 MOD/CMD RCVD 196CFG
electrical input requirements, 3.1-1 DLD, 7.1-18
INDEX
INVALID MOD/CMD IN DLTR DWNLD, 7.1-18 Laser Power Supply buck-boost transformer
INVALID MOD/CMD RCVD 376CFG jumpers and connections, table, A.3-4
DWNLD, 7.1-18 latex aspiration modes
INVALID MOD/CMD RCVD IN DLTR Diluter flow, 2.11-8
DWNLD, 7.1-18 latex calibration
INVALID SYSTEM COMMAND, 7.1-18 CBC. See calibration, CBC
diff. See calibration, VCS
retic. See calibration, VCS
K LATRON control
kits calibration and verification limits, A.1-8
options and upgrades, part numbers, 8.1-16 LATRON primer
Service Resource, part number, 1.1-1 background limits, A.1-8
leakage current specifications
L computers, such as DMS, 2.1-5
instrument, 2.1-5
laboratory information, setting up, 3.5-1
left stack. See unloading bay
lamps
lens block
Hgb. See Hgb lamp
cleaning procedure, 4.14-1
optic. See optic lamps
laser
LESS THAN 1 MEGABYTE LEFT ON DRIVE
and flow cell alignment procedure, 4.15-1
C, 7.1-19
and flow cell alignment procedure problems,
level sensors. See sensors, level
troubleshooting, 4.15-1
lift. See load elevator; unload elevator
and flow cell alignment work flow,
light scatter (LS or S)
illustration, 4.15-1
VCS measurement, description, 2.13-1
documents, regulatory, 1.2-2
limits and tolerances. See specifications
in TTM, radiation classification of, 1.2-2
linear light scatter (LLS)
TTM, location, illustration, 2.13-3
VCS measurement, description, 2.13-2
laser current on
load elevator
acceptable limits, A.1-8
laser hazards
load elevator down sensor
light beam, 1.2-1
personal injury from laser beam, prevention
of, 1.2-1
personal injury from radiation, prevention
of, 1.2-2
LOAD ELEVATOR FAILURE, 7.1-19
radiation, 1.2-2
load elevator motor
Laser Power Supply
buck-boost transformer jumpers and
connections, table, A.3-4
replacement procedure, 4.36-1
configuration criteria for ac line input,
load stack empty sensor
table, A.3-4
configuring Laser Power Supply Buck Boost
Transformer at instrument installation, 3.2-4
function, 2.2-4
location, illustration, 2.9-3, 2.9-4
LOAD STACK NOT EMPTY, 7.1-19
voltages monitored by I/O card, 2.4-7
load stack. See loading bay
Laser Power Supply Buck Boost Transformer
loading bay
configuring at instrument installation, 3.2-4
function, 2.9-1
location, illustration, 2.9-3, 2.9-4
INDEX
low vacuum Main Diluter module

acceptable range, A.1-5 component locations, A.5-9, A.5-10
adjustment procedure, 4.25-6 component part numbers, illustrated parts
distribution, 2.5-3 list, 8.2-41
regulator. See RG1 (low vacuum regulator) component part numbers, master parts
LOW VACUUM OUT OF RANGE, 7.1-19 list, 8.1-11
LOWER DOOR OPEN, 7.1-19 location, illustration, 2.1-3
LS (light scatter or S) main power switch
VCS measurement, description, 2.13-1 See POWER switch
LS (S or light scatter) Main Unit
troubleshooting latex calibration cable connections, illustration, 3.3-1
problems, 7.4-1 dimensions, 3.1-1
LS offset voltage fan and vent locations, 3.1-2
acceptable limits, A.1-8 left-side view, main components, 2.1-4
measuring at instrument installation, 3.7-1 rear view, main components, 2.1-3
measuring, procedure, 4.23-3 right-side view, main components, 2.1-3
LS Preamp 5 module with Autoloader module, front view, main
block diagram, 2.13-7 components, 2.1-2
function, 2.13-6 with Rotary Cap-Pierce module, front view,
function of the Diff/Retic Gain Select main components, 2.1-2
circuitry, 2.13-7 manifolds
inputs/outputs, 2.13-8 location references, A.5-3
location, illustration, 2.13-3 manual, 1.1-1
LS Offset voltage measurement, 2.13-7 abbreviations used in, ABBREVIATIONS-1
LS Preamp module conventions used, 1.1-4
routes pulses to VCS PROCESSOR card, 2.4-9 for servicing the DMS, 1.1-1
LS sensor intended audience, 1.1-1
location, illustration, 2.13-3 numbering format, 1.1-2
LV (solenoids) obtaining online version, 1.1-1
location references, table, A.5-3 organization, 1.1-2
table of functions and location references, 4.26-1 reference designators used
See also solenoids in, ABBREVIATIONS-1
LY% scope, 1.1-1
verifying reproducibility in the Primary special headings defined, 1.1-3
mode, 4.5-1 manuals, customer
verifying reproducibility in the Secondary part numbers, 1.1-1
mode, 4.5-2 MAXM analyzer. See instrument
LY% and LY# MAXM TX BUFF REQUEST NOT
part of reported WBC differential, 2.1-1 SUCCESSFUL, 7.1-19
See also diff parameters MC1 (diff mixing chamber)
lymphocytes. See LY function, A.5-3
LYSE OUT, 7.1-19 location, illustration, A.5-10
LYSE TRIG (lyse trigger) test point MCV
location/function, 2.4-4 method used for determining, 2.12-1
lytic reagent. See CBC lytic reagent; diff lytic reagent verifying reproducibility in the Primary
mode, 4.5-1
verifying reproducibility in the Secondary
M mode, 4.5-2
M1. See rocker bed motor voting criteria, 2.4-13
M2. See cassette index motor
INDEX
MEMORY ERROR, 7.1-20 MO%

meniscus verifying reproducibility in the Primary
reading correctly, illustration, 4.16-5, 4.19-2 mode, 4.5-1
menu trees, software. See software menu trees verifying reproducibility in the Secondary
MF1 mode, 4.5-2
function, A.5-3 MO% and MO#
location, illustration, A.5-9 part of reported WBC differential, 2.1-1
MF2 See also diff parameters
function, A.5-3 mode-to-mode
location, illustration, A.5-7 calibration procedure, 4.8-1
MF3 comparison limits, A.1-4
function, A.5-4 monocytes. See MO
location, illustration, A.5-8 motors
MF5 B1 (load elevator motor). See load elevator
function, A.5-4 motor
location, illustration, A.5-11, A.5-12 B2 (unload elevator motor). See unload elevator
MF6 motor
function, A.5-4 cassette index. See cassette index motor
location, illustration, A.5-11, A.5-12 load elevator. See load elevator motor
MF7 mixing. See mixing motor
function, A.5-4 rocker bed. See rocker bed motor
location, illustration, A.5-11, A.5-12 unload elevator. See unload elevator motor
MF8 MPV
function, A.5-4 method used for determining, 2.12-1
location, illustration, A.5-9 verifying reproducibility in the Primary
MF10 mode, 4.5-1
function, A.5-4 verifying reproducibility in the Secondary
MF11 voting criteria, 2.4-13
function, A.5-4 MULT. INTER. WHILE RECEIVING DATA, 7.1-20
location, illustration, A.5-10 MULTIPLE ERRORS, 7.1-20
MF14
function, A.5-4
location, illustration, A.5-10 N
MF15 NE%
function, A.5-4 verifying reproducibility in the Primary
MF17 verifying reproducibility in the Secondary
function, A.5-4 mode, 4.5-2
location, illustration, A.5-10 NE% and NE#
Mixing module part of reported WBC differential, 2.1-1
component locations, A.5-10, A.5-11 See also. diff parameters
component part numbers, illustrated parts needle
list, 8.2-7, 8.2-9, 8.2-11 adjusting the position of where it penetrates the
component part numbers, master parts tube stopper, 4.41-1
list, 8.1-15 replacement procedure, Autoloader
location, illustration, 2.1-3 module, 4.32-1
mixing motor replacement procedure, Rotary Cap-Pierce
location, illustration, A.5-11 module, 4.32-5
needle assembly
INDEX
function, 2.8-1, 2.9-1 orientation on needle-drive cylinder,

location, illustration, 2.8-2, 2.9-3, 2.9-4 illustration, 4.44-1
needle-drive cylinder NEEDLE HOME SENSOR ERROR, 7.1-20
adjusting to achieve optimal horizontal needle vent chamber
tube-piercing position, 4.41-2 function, A.5-6
location, illustration, Autoloader module location, illustration, A.5-11, A.5-12
(CL6), 2.9-4 neutrophils See NE
location, illustration, Rotary Cap-Pierce module NO PARAMETER SELECTED, 7.1-20
(CL5), 2.8-2 noise, electronic. See electronic noise
needle forward sensor normal position sensor
action to change state, Autoloader module action to change state, 4.43-6
(S1), 4.43-2 function and location reference, 4.43-6
action to change state, Rotary Cap-Pierce function, type, and location reference, 2.8-2
module (U2), 4.43-5 location on code wheel, illustration, 4.31-2
adjustment, 4.44-1 location, illustration, 2.8-2
function and location reference, Autoloader Note
module (S1), 4.43-2 heading defined, 1.1-3
function and location reference, Rotary Notice of Information Update form
Cap-Pierce module (U2), 4.43-5 when used, 1.1-1
function, type, and location reference,
Autoloader module (S1), 2.9-2
function, type, and location reference, Rotary O
Cap-Pierce module (U2), 2.8-2 online service manuals, obtaining, 1.1-1
location, illustration, Autoloader module OP (opacity)
(S1), 2.9-4 VCS measurement, description, 2.13-2
location, illustration, Rotary Cap-Pierce module opacity (OP)
(U2), 2.8-2 VCS measurement, description, 2.13-2
orientation on needle-drive cylinder, optic lamps
illustration, 4.44-1 function, A.5-1
NEEDLE FORWARD SENSOR ERROR, 7.1-20 location, illustration, A.5-8
needle home sensor options and upgrade kits
action to change state, Autoloader module part numbers, 8.1-16
(S0), 4.43-2 overflow chamber
action to change state, Rotary Cap-Pierce function, A.5-6
module (U1), 4.43-5 location, illustration, A.5-8
adjustment, 4.44-1 oversized pos. sensor
function and location reference, Autoloader action to change state, 4.43-6
module (S0), 4.43-2 function and location reference, 4.43-6
function and location reference, Rotary function, type, and location reference, 2.8-2
Cap-Pierce module (U1), 4.43-5 location, illustration, 2.8-2
function, type, and location reference, oversized position sensor
Autoloader module (S0), 2.9-2 location on code wheel, illustration, 4.31-2
function, type, and location reference, Rotary
Cap-Pierce module (U1), 2.8-2 P
location, illustration, Autoloader module
PAK LYSE. See diff lytic reagent; Erythrolyse II
(S0), 2.9-4
reagent
location, illustration, Rotary Cap-Pierce module
PAK OUT, 7.1-20
(U1), 2.8-2
PAK PRESERVE. See diff leukocyte preservative;
StabiLyse reagent
INDEX
parameters reported shielded reagent lines, 8.1-21

CBC, 2.1-1 software, 8.1-21
for research use only, 2.1-1 tools and supplies, 8.1-22
reticulocyte, 2.1-1 Triple Transducer module, 8.1-23
WBC differential, 2.1-1 tubing, 8.1-25
part numbers PARTIAL ASPIRATION, 7.1-21
customer manuals, 1.1-1 parts lists
DMS Configuration Listing for STKS and MAXM master, 8.1-1
Series Systems, 1.1-1 PATIENT ID REQUIRED FOR PROCESSING, 7.1-21
instrument components. See part numbers, PC1, 7.2-1
illustrated parts lists; part numbers, master PC2, 7.2-2
parts lists Peltier Controller card
MAXM Instrument Reference Form, 5.1-1 block diagram, 2.6-3
RECOMMENDED MAXM INSTRUMENT connector and LED locations, illustration, A.2-6
MAINTENANCE SCHEDULE, 5.1-1 connectors and LED functions, table, A.2-6
Service Resource Kit, 1.1-1 function, A.5-1
System Verification Procedure form (SVP), 5.1-1 location, illustration, A.5-8
part numbers, illustrated parts lists Peltier module
Analyzer module, 8.2-47 description, 2.6-3
Autoloader function, 2.6-3
module, 8.2-29, 8.2-31, 8.2-33, 8.2-35 location, illustration, A.5-8
BSV module, 8.2-13, 8.2-15, 8.2-17, 8.2-19 signals enabled by the I/O card, 2.4-7
CBC module, 8.2-3, 8.2-5 See also Peltier Controller card
electrical connectors, 8.2-50 pickup tubes and sensors
Electronic Power Supply, 8.2-43 part numbers, 8.1-16
Main Diluter module, 8.2-41 pierce position sensor
Mixing module, 8.2-7, 8.2-9, 8.2-11 action to change state, 4.43-3
Pneumatic Power Supply, 8.2-45 function and location reference, 4.43-3
Pump module, 8.2-21, 8.2-23 function, type, and location reference, 2.9-3
Rotary Cap-Pierce module, 8.2-25, 8.2-27 location, illustration, 2.9-4
Triple Transducer module pinch valves
(TTM), 8.2-37, 8.2-39 deactivator clips, removal, 3.2-2
wire harness components, 8.2-49 location references, A.5-5
part numbers, master parts lists with inconspicuous deactivator clips, table
accessories, instrument, 8.1-1 of, 3.2-2
Autoloader module, 8.1-2 platelet count. See Plt
bar-code system components, 8.1-4 platelet. See Plt
BSV module, 8.1-5 PLB1 (Peltier module)
CBC module, 8.1-7 location, illustration, A.5-8
circuit cards, 8.1-8 See also Peltier module
covers and doors, 8.1-9 Plt
Diluter hardware, miscellaneous, 8.1-12 checking carryover in the Primary mode, 4.6-1
interconnect cables, 8.1-10 histogram development, 2.4-10
Main Diluter module, 8.1-11 histogram particle accumulation, 2.4-10
Mixing module, 8.1-15 method used for determining, 2.12-1
options and upgrade kits, 8.1-16 reports 0.00; software download not
pickup tubes and sensors, 8.1-16 successful, 7.5-1
power supplies, 8.1-17 test point for measuring noise level, 2.4-13
Pump module, 8.1-18 verifying reproducibility in the Primary
Rotary Cap-Pierce module, 8.1-19 mode, 4.5-1
INDEX
verifying reproducibility in the Secondary component locations, illustration, A.2-7

mode, 4.5-2 connector, A.2-7
voting criteria, 2.4-13 function, 2.5-4
PM1 (CBC lytic reagent pump) hazard of adjusting the voltage
function, A.5-4 potentiometers, 4.25-1
location, illustration, A.5-8 location, illustration, 2.1-3, A.5-10
See also CBC lytic reagent pumps test point voltages for pneumatic readings,
PM2 (CBC lytic reagent pump) conversion table, A.2-8
function, A.5-4 used for monitoring 30-psi pressure
location, illustration, A.5-8 adjustment, 4.25-3
See also CBC lytic reagent pumps used for monitoring 60-psi pressure
PM3 (air pump) adjustment, 4.25-1
function, A.5-4 used for monitoring low vacuum
location, illustration, A.5-7 adjustment, 4.25-6
PM4 (aspiration pump, Primary mode) used for monitoring sample-pressure
function, A.5-4 adjustment, 4.25-5
location, illustration, A.5-7, A.5-11, A.5-12 used for monitoring sheath-pressure
See also aspiration pump, Primary mode adjustment, 4.25-4
PM5 (aspiration pump, Secondary mode) used for verifying high vacuum, 4.25-5
function, A.5-4 Pneumatic Power Supply
location, illustration, A.5-7, A.5-11, A.5-12 Buck-Boost Transformer Terminal card jumpers
PM6 (Erythrolyse II reagent pumps) and connections, table, A.3-4
function, A.5-4 component part numbers, illustrated parts
location, illustration, A.5-11, A.5-12 list, 8.2-45
See also Erythrolyse II reagent pumps compressor bleed, monitoring sequence, 2.5-5
PM7 (Erythrolyse II reagent pumps) configuration criteria for ac line input,
function, A.5-4 table, A.3-4
location, illustration, A.5-11, A.5-12 configuring Pneumatic Power Supply
See also Erythrolyse II reagent pumps Buck-Boost Transformer Terminal card at
PM8 (backwash pump) instrument installation, 3.2-3
function, A.5-4 description, 2.5-1
location, illustration, A.5-10 function, 2.5-1
PM9 (WBC diluent dispenser) fuse, 2.5-2
function, A.5-5 houses intake fan, 3.1-2
location, illustration, A.5-10 location, illustration, 2.1-3, 2.1-4
See also WBC diluent dispenser main components, 2.5-1
PM10 (cleaning agent pump) main components, illustration, 2.5-1
function, A.5-5 replacement procedure, 4.24-1
location, illustration, A.5-10 shipping pins, illustration, 3.2-3
PM11 (RBC diluent dispenser) shipping pins, removal, 3.2-3
function, A.5-5 voltage configurations, 2.5-2
location, illustration, A.5-10 Pneumatic Power Supply Buck-Boost Transformer
See also RBC diluent dispenser Terminal card
PM12 (StabiLyse reagent pump) configuring at instrument installation, 3.2-3
function, A.5-5 jumpers and connections, table, A.3-4
location, illustration, A.5-10 Pneumatic system
See also StabiLyse reagent pump compressor bleed monitoring sequence, 2.5-5
Pneumatic Monitor card function, 2.5-1
block diagram, 2.5-4
INDEX
Pneumatic Monitor card. See Pneumatic Monitor verifying reproducibility and carryover at
card instrument installation, 3.7-2
Pneumatic Power Supply. See Pneumatic Power See also aspiration pump, Primary mode
Supply Printer Logic Control card
pneumatic readings converted to voltages, DIP switches settings, 3.9-1
table, A.2-8 self-test switch location, 3.9-1
pressure and vacuum adjustments, 4.25-1 Printer, Graphic
pressure and vacuum distribution and cable connections, illustration, 3.3-1
monitoring, 2.5-2 fan location, 3.1-2
power installation procedure, reference, 3.9-1
consumption, typical, A.1-6 Printer, Ticket
input specifications, A.1-5 Anadex installation, 3.9-1
power down/power up procedures, 4.1-2 cable connections, illustration, 3.3-1
power supplies PRN TX BUFF REQUEST NOT
electronic. See Electronic Power Supply SUCCESSFUL, 7.1-21
laser. See Laser Power Supply probe
part numbers, 8.1-17 aspirator. See aspirator tip
pneumatic. See Pneumatic Power Supply bleach. See bleach probe
RF. See RF Power Supply procedures
POWER switch activating the DMS service options, 4.2-1
function, 2.2-1 adjustment. See adjustment procedures
location, 2.1-3 Bar-Code Reader Decoder card
precautions, safety, 1.2-1 configuration, 4.38-1
precision. See reproducibility BSV and housing verification, 4.29-11
preinstallation checks, 3.1-1 BSV disassembly, 4.29-1
pressure tank carryover, 4.6-1
function, 2.5-2 CBC calibration. See calibration, CBC
location, illustration, 2.5-2 checking electronic noise levels, 4.12-1, 7.3-1
pressures cleaning. See cleaning procedures
30 psi. See 30-psi pressure configuring the instrument for cyanide-free
60 psi. See 60-psi pressure reagents, 3.10-1
sample. See sample pressure CPU RAM clearance, 4.1-3
sheath. See sheath pressure installation, instrument. See installation
PREVIOUS SAMPLE NOT XMITTED TO procedures, instrument
HOST, 7.1-21 installation, options and upgrades. See
Primary mode installation procedures, options and
checking carryover, 4.6-1 upgrades
determining initial calibration factors, 4.7-1 instrument reset, 4.1-2
Diluter flow, 2.11-4 measuring the LS offset voltage, 4.23-3
to Secondary comparison limits, A.1-4 power down/power up, 4.1-2
verifying operation at instrument installation removal. See removal procedures
(on instruments with a Rotary Cap-Pierce replacement. See replacement procedures
module), 3.6-2 reproducibility, 4.5-1
verifying operation at instrument installation sensor and switch checks, 4.43-1
(on instruments with an Autoloader solenoid inspection, 4.26-1
module), 3.6-3 system verification, 5.1-1
verifying reproducibility, 4.5-1 using the service disk 2 software, 4.2-3
using the service disk 2 utilities, 4.2-10
VCS calibration. See calibration, VCS
INDEX
VCS optimization. See VCS optimization R

whole blood verification, 4.3-1 R/W PREAMP card
Pump module block diagram, 2.4-11
component locations, A.5-11, A.5-12 function, 2.4-14
component part numbers, illustrated parts inputs/outputs, 2.4-14
list, 8.2-21, 8.2-23 location, illustration, 2.4-1
component part numbers, master parts role in histogram development, 2.4-10
list, 8.1-18 sends pulses to the R/W/PROC card, 2.4-10
location, illustration, 2.1-2 supplies aperture current to apertures, 2.12-1
pumps R/W/P PROC card
diaphragm style, adjustment, illustration, 4.16-5 block diagram, 2.4-11
PM1 (CBC lytic reagent). See CBC lytic reagent function, 2.4-9
pumps inputs/outputs, 2.4-13
PM2 (CBC lytic reagent). See CBC lytic reagent location, illustration, 2.4-1
pumps RBC, WBC, Plt test points
PM4 (aspiration pump, Primary mode). See location/function, 2.4-13
aspiration pump, Primary mode role in histogram development, 2.4-10
PM6 (Erythrolyse II reagent pump). See radiation, laser
Erythrolyse II reagent pumps hazards, 1.2-2
PM7 (Erythrolyse II reagent pump). See RAM Timer card
Erythrolyse II reagent pumps function/description, 2.4-3
PM9 (WBC diluent dispenser). See WBC diluent jumper locations, illustration, A.2-9
dispenser jumper settings, A.2-9
PM11 (RBC diluent dispenser). See RBC diluent RAW DATA SPACE FULL-CAPTURE OFF, 7.1-22
dispenser RAW DATA SWITCH OFF, 7.1-22
PM12 (StabiLyse reagent pump). See StabiLyse RAW DATA TRANSMISSION ERROR, 7.1-22
reagent pump RAW FILE TOO LARGE, 7.1-22
RB1 (rinse block)
Q function, A.5-5
Q1 (bar-code detector sensor). See bar-code location, illustration, A.5-7
detector sensor RBC
Q2 (hand detector sensor). See hand detector checking carryover in the Primary mode, 4.6-1
QD1F, location, illustration, A.5-9 counting sequences, 2.4-10
QD1M, location, illustration, A.5-9 histogram development, 2.4-10
QD2F, location, illustration, A.5-9 histogram particle accumulation, 2.4-10
QD2M, location, illustration, A.5-7 method used for determining, 2.12-1
QD3F, location, illustration, A.5-9 reports 0.00;software download not
QD3M, location, illustration, A.5-7 successful, 7.5-1
QD4F, location, illustration, A.5-9 test point for measuring noise level, 2.4-13
QD5F, location, illustration, A.5-9 verifying reproducibility in the Primary
QD5M, location, illustration, A.5-11, A.5-12 mode, 4.5-1
QD6F, location, illustration, A.5-9 verifying reproducibility in the Secondary
QD6M, location, illustration, A.5-10 mode, 4.5-2
QD7, location, illustration, A.5-10 voting criteria, 2.4-10
QD8, location, illustration, A.5-10 RBC AND WBC BATH OVERFLOW, 7.1-22
QD9, location, illustration, A.5-10 RBC bath
QD10, location, illustration, A.5-10 function, A.5-2
QD11, location, illustration, A.5-10 location, illustration, A.5-8
quench. See StabiLyse reagent
INDEX
replacement, 4.28-1 waste removal and monitoring, 2.6-5

See also Aperture module and bath assembly See also Fluid Detector/Ram Pressure card;
RBC BATH OVERFLOW, 7.1-22 Peltier module
RBC diluent dispenser RECOMMENDED MAXM INSTRUMENT
function, A.5-5 MAINTENANCE SCHEDULE, part
location, illustration, A.5-10 number, 5.1-1
measuring volume dispensed by volume, 4.19-1 RED A/I/V OUT OF RANGE, 7.1-22
measuring volume dispensed by weight, 4.19-2 red blood cell count. See RBC
volume adjustment procedure, 4.19-1 red blood cell. See RBC
volume tolerances, A.1-10 reference designators
RBC vacuum isolator chamber used in this manual, ABBREVIATIONS-1
function, A.5-6 regulators
location, illustration, A.5-8 60-psi pressure. See 60-psi pressure regulator
RBC VALUE MUST BE > 0 AND < OR EQUAL TO RG1 (low vacuum). See low vacuum regulator
9.99, 7.1-22 RG2 (30-psi pressure). See 30-psi pressure
RBC VALUE MUST BE > 0 AND < OR EQUAL TO regulator
999, 7.1-22 RG3 (sheath pressure). See sheath-pressure
RCP Junction card regulator
block diagram, 2.7-3 RG4 (sample pressure). See sample-pressure
function, 2.8-4 regulator
inputs/outputs, 2.8-5 removal procedures
location, illustration, 2.8-2 Autoloader module, 4.34-1
RDW rocker bed, 4.35-1
verifying reproducibility in the Primary replacement procedures
mode, 4.5-1 Aperture module and bath assembly, 4.28-1
verifying reproducibility in the Secondary BSV, 4.29-1
mode, 4.5-2 BSV actuator, 4.29-6
voting criteria, 2.4-13 BSV housing, 4.29-5
reagent lines, shielded. See shielded reagent lines carousel motor, 4.30-1
Reagent system Electronic Power Supply, 4.22-1
configuring for cyanide-free reagents, 3.10-1 elevator stepper motors, 4.36-1
enabling/disabling the level sensors, 3.5-1 Erythrolyse II reagent pumps, 4.16-8
erroneous reagent out alarms, 7.5-2 Hgb Preamp module, 4.27-1
precautions for ensuring correct reagent and needle, Autoloader module, 4.32-1
waste connections, 3.3-5 needle, Rotary Cap-Pierce module, 4.32-5
precautions for preventing introduction of Pneumatic Power Supply, 4.24-1
electronic noise, 3.3-4 solenoids, 4.26-6
priming reagents, 3.5-1 tube available sensor, 4.37-1
reagent and drain connections, 3.3-4 reproducibility
reagent and drain connections, acceptable limits, A.1-3
illustration, 3.3-4 checking, parameter requirements of specimens
reagent input and distribution, 2.6-1 used, A.1-3
reagent monitoring, 2.6-1 procedures, 4.5-1
reagents used, 2.6-1 REPRODUCIBILITY IS ACTIVE, 7.1-22
setup at instrument installation, 3.5-1 REPRODUCIBILITY TABLE FULL, 7.1-22
temperature control of Erythrolyse II reproducibility, Primary mode
reagent, 2.6-3 acceptable limits, A.1-4
temperature control of mixing chamber rinse checking, procedure, 4.5-1
diluent, 2.6-3 verifying at instrument installation, 3.7-2
waste drainage requirements, 3.1-3
INDEX
reproducibility, Retic mode signals monitored by DILUTER INTERFACE

acceptable limits, A.1-4 card, 2.4-4
checking, procedure, 4.5-3 supplies DC and RF currents, 2.13-2
verifying at instrument installation, 3.7-3 RF Power Supply
reproducibility, Secondary mode components, 2.2-4
acceptable limits, A.1-4 function, 2.2-4
checking, procedure, 4.5-2 location, illustration, 2.2-3
verifying at instrument installation, 3.7-2 RF Power Supply card
reset procedure, 4.1-2 function, 2.2-4
RET% and RET# RF VOLTAGE LOW, 7.1-23
reticulocyte parameters reported by RF/DC Detector Preamp card
instrument, 2.1-1 possible cause of an FC error, 7.2-1
See also retic parameters RG1 (low vacuum regulator)
retic latex calibration. See calibration, VCS function, A.5-5
Retic mode location, illustration, A.5-10
checking carryover, 4.6-2 RG2 (30-psi pressure regulator)
verifying reproducibility, 4.5-3 function, A.5-5
verifying reproducibility and carryover at location, illustration, A.5-10
instrument installation, 3.7-3 RG3 (sheath-pressure regulator)
retic parameter function, A.5-6
checking carryover, 4.6-2 location, illustration, A.5-10
noise checks, 4.12-1 RG4 (sample-pressure regulator)
verifying reproducibility, 4.5-3 function, A.5-6
retic parameters location, illustration, A.5-10
method used for determining, 2.13-1 right stack. See loading bay
reported by instrument, 2.1-1 rinse block
See also VCS technology adjustment procedure, 4.33-1
RETIC VOLTAGE ERROR [XX.XX], 7.1-23 arm sensor. See rinse block arm sensor
Reticulocyte mode avoiding physical damage while
Diluter flow, 2.11-8 adjusting, 4.33-1
reticulocytes. See RET; retic rinse block arm sensor
RETRIES EXCEEDED IN DILUTER DWNLD, 7.1-23 action to change state, Autoloader
RETRIES FAILED 196CODE DWNLD TO module, 4.43-1
376, 7.1-23 action to change state, Rotary Cap-Pierce
RF (C or conductivity) module, 4.43-5
troubleshooting latex calibration function and location reference, Autoloader
problems, 7.4-1 module, 4.43-1
VCS measurement, description, 2.13-1 function and location reference, Rotary
RF Detector Preamp card Cap-Pierce module, 4.43-5
300 Vac input controlled by DILUTER RINSE BLOCK ERROR, 7.1-23
INTERFACE card, 2.4-4 RLS (rotated light scatter)
adjustment procedures, 4.11-1 VCS measurement, description, 2.13-2
function, 2.13-5 rocker bed
inputs/outputs, 2.13-6 function, 2.9-1
location, illustration, 2.13-3 function of the adjustable linkage, 4.40-1
receives voltage from RF Power Supply linkage adjustment procedure, 4.40-1
card, 2.2-4 linkage adjustment, illustration, 4.40-2
routes pulses to VCS PROCESSOR card, 2.4-9 location, illustration, 2.9-3
signal flow, illustration, 2.13-6
INDEX
position sensors. See horizontal position sensor; VCS measurement, description, 2.13-1
pierce position sensor S0 (needle home sensor). See needle home sensor
removal procedure, 4.35-1 S1 (needle forward sensor). See needle forward
Rocker Bed Interface card sensor
block diagram, 2.7-2 S2 (cassette position 1 sensor). See cassette position
connector locations, illustration, A.2-10 1 sensor
connectors, table, A.2-10 S2-whole blood (whole blood switch). See whole
function, 2.9-8 blood switch
inputs/outputs, 2.9-9 S3-backwash (backwash safety sensor). See
location, illustration, 2.9-4 backwash safety sensor
rocker bed motor S3 (cassette position 2 sensor). See cassette position
function, type, and location reference, 2.9-2 2 sensor
location, illustration, 2.9-4 S4 (rinse block arm sensor). See rinse block arm
ROCKER BED NOT EMPTY, 7.1-23 sensor
Rotary Cap-Pierce module S4-stop (STOP switch). See STOP switch
block diagram of its associated circuit S5 (door interlock, Autoloader module). See door
cards, 2.7-3 interlock
carousel assembly. See carousel assembly S5-tank (sheath tank sensor). See sheath tank
carousel position indicated by LEDs on Sample sensor
Handler card, 2.7-4 S6 (unload elevator down sensor). See unload
component part numbers, illustrated parts elevator down sensor
list, 8.2-25, 8.2-27 S7 (tube available sensor). See tube available sensor
component part numbers, master parts S8 (load elevator down sensor). See load elevator
list, 8.1-19 down sensor
description, 2.8-1 S9 (full cass index rotation sensor). See full cass
ensuring Sample Handler card is configured index rotation sensor
for, 2.7-4 S10 (tube ram sensor) See tube ram sensor
function, 2.8-1 S11 (unload stack full sensor). See unload stack full
location, illustration, 2.1-2 sensor
main component descriptions, table of, 2.8-1 S12 (cassette position 0 sensor). See cassette
main component locations, illustration, 2.8-2 position 0 sensor
needle replacement procedure, 4.32-5 S13 (cassette position 3 sensor). See cassette
operation, summary, 2.8-3 position 3 sensor
preventing damage to during software S14 (tube forward sensor). See tube forward sensor
download, 2.7-4 S15 (load stack empty sensor). See load stack empty
RCP Junction card. See RCP Junction card sensor
sensor and switch functions and location S16 (pierce position sensor). See pierce position
references, table of, 4.43-5 sensor
rotated light scatter (RLS) S17 (horizontal position sensor). See horizontal
VCS measurement, description, 2.13-2 position sensor
RWP PROC card S18 (door interlock, Rotary Cap-Pierce module).
component locations, illustration, A.2-11 See door interlock
jumper settings, table, A.2-11 safety precautions, 1.2-1
test points functions, table, A.2-11 biological, 1.2-3
electronic, 1.2-3
laser beam, 1.2-1
S laser radiation, 1.2-2
S (light scatter or LS) troubleshooting, 1.2-4
troubleshooting latex calibration
problems, 7.4-1
INDEX
Sample Analysis system. See CBC technology; VCS SAMPLE HANDLER SENSOR 16 ERROR, 7.1-24
technology SAMPLE HANDLER SENSOR 17 ERROR, 7.1-24
Sample Handler Sample Handler system. See Autoloader module;
card. See Sample Handler card Autosensor Test card; Rotary Cap-Pierce
module. See Autoloader module; Rotary module; Sample Handler card
Cap-Pierce module SAMPLE HANDLER TIMEOUT ERROR, 7.1-24
SAMPLE HANDLER BARCODE SENSOR SAMPLE NOT TRANSMITTED TO HOST, 7.1-25
ERROR, 7.1-24 sample pressure
Sample Handler card adjustment procedure, 4.10-1
acceptable blood/bubble detector voltages, A.1-9 distribution, 2.5-3
avoiding a short circuit on when aligning the function, 2.13-4
code wheel, 4.31-1 initial adjustment procedure, 4.25-5
controls solenoids in the Sample Handler See also diff pressure
module, 2.11-1 sample-pressure regulator
ensuring configuration matches Sample Handler function, A.5-6
on instrument, 2.7-4 location, illustration, A.5-10
function, 2.7-1 Sample Processing system. See Diluter
inputs/outputs, 2.7-5 S-CAL calibration
LED functions, 2.7-1 performed by customer at instrument
LED responses during download, 2.7-3 installation, 3.7-4
LED responses during power up, 2.7-1 scanner, bar-code. See bar-code scanner
LED responses to the carousel position in the schematics, engineering. See engineering
Rotary Cap-Pierce module, 2.7-4 schematics
LEDs response to a power fluctuation, 2.7-4 screens, aperture viewing
location, illustration, 2.1-4 function, A.5-1
provides hazard control for Sample Handler location, illustration, A.5-8
module during software download, 2.7-4 SEC CBC CALIBRATION TABLE FULL, 7.1-25
with Autoloader module, block diagram, 2.7-2 Secondary mode
with Rotary Cap-Pierce module, block checking carryover, 4.6-2
diagram, 2.7-3 determining calibration factors, 4.8-1
SAMPLE HANDLER COMM. FAILURE, 7.1-24 Diluter flow, 2.11-7
SAMPLE HANDLER COMMUNICATION to Primary mode calibration, verifying at
ERROR, 7.1-24 instrument installation, 3.7-3
SAMPLE HANDLER HAND DETECT ERROR, 7.1-24 to Primary mode comparison limits, A.1-4
Sample Handler I card verifying operation at instrument
component locations, illustration, A.2-12 installation, 3.6-5
connectors, table, A.2-12 verifying reproducibility, 4.5-2
jumper settings, table, A.2-13 verifying reproducibility and carryover at
LED functions, table, A.2-13 instrument installation, 3.7-2
test points and adjustments for the blood/bubble SEND TO INSTRUMENT FAILED -
detectors, A.2-12 196CODE, 7.1-25
Sample Handler II card sensors
component locations, illustration, A.2-14 ambient temperature. See ambient temperature
connectors, table, A.2-14 sensor
jumper settings, table, A.2-14 checking procedure, 4.43-1
LED functions, table, A.2-15 DS1 (hand detector). See hand detector sensor
test points and adjustments for the blood/bubble DS2 (bar-code detector). See bar-code detector
detectors, A.2-14 sensor
SAMPLE HANDLER NOT OPERATIONAL, 7.1-24
INDEX
FD3 (Erythrolyse reagent). See Erythrolyse U2 (needle forward). See needle forward sensor
(reagent) sensor U3 (normal position). See normal position
FD4 (Stabilyse reagent). See Stabilyse (reagent) sensor
sensor U4 (oversized pos.). See oversized pos.sensor
FD5 (CBC lyse). See CBC lyse sensor U5 (tube available). See tube available sensor
FD6 (diluent). See diluent sensor waste. See waste sensor
FD7 (cleaner). See cleaner sensor See also switches
in instruments with Autoloader module, sensors, level
table, 4.43-1 enabling/disabling, 3.5-1
in instruments with Rotary Cap-Pierce module, sensing reagent levels incorrectly, 7.5-2
table, 4.43-5 used for monitoring reagents, 2.6-1
level. See sensors, level used for monitoring waste, 2.6-5
Q1 (bar-code detector). See bar-code detector See also CBC lyse sensor; cleaner sensor; diluent
sensor sensor; Erythrolyse (reagent) sensor; sheath
Q2 (hand detector). See hand detector sensor tank sensor; Stabilyse (reagent) sensor; waste
S0 (needle home). See needle home sensor sensor
S1 (needle forward). See needle forward sensor service
S2 (cassette position 1). See cassette position 1 disk. See service disk
sensor forms provided in manual, 5.1-1
S3-backwash (backwash safety). See backwash guidelines, 4.1-1
safety sensor tools and supplies, part numbers, 8.1-22
S3 (cassette position 2). See cassette position 2 service disk
sensor activating the DMS service options, 4.2-1
S4 (rinse block arm). See rinse block arm sensor deactivating the DMS service options, 4.2-2
S5 (door interlock, Autoloader module). See using the software on the service disk 2, 4.2-3
door interlock utilities menu tree for the service disk 2, 4.2-9
S5-tank (sheath tank). See sheath tank sensor utilities selections for the service disk 2,
S6 (unload elevator down). See unload elevator descriptions and procedures, 4.2-10
down sensor SERVICE REPRODUCIBILITY TABLE FULL, 7.1-25
S7 (tube available). See tube available sensor Service Resource Kit
S8 (load elevator down). See load elevator down part number, 1.1-1
sensor SF1 (sweep-flow tank)
S9 (full cass index rotation). See full cass index function, A.5-6
rotation sensor location, illustration, A.5-8
S10 (tube ram). See tube ram sensor sheath fluid
S11 (unload stack full). See unload stack full function, 2.13-3
sensor sheath pressure
S12 (cassette position 0). See cassette position 0 acceptable range, A.1-5
sensor adjustment procedure, 4.10-1, 4.25-4
S13 (cassette position 3). See cassette position 3 distribution, 2.5-3
sensor function, 2.13-3
S14 (tube forward). See tube forward sensor SHEATH PRESSURE OUT OF RANGE, 7.1-25
S15 (load stack empty). See load stack empty sheath-pressure regulator
sensor function, A.5-6
S16 (pierce position). See pierce position sensor location, illustration, A.5-10
S17 (horizontal position). See horizontal sheath tank
position sensor function, A.5-6
S18 (door interlock, Rotary Cap-Pierce module). location, illustration, A.5-7
See door interlock sensor. See sheath tank sensor
U1 (needle home). See needle home sensor SHEATH TANK EMPTY, 7.1-25
INDEX
sheath tank sensor function in the Diluter, 2.11-1

action to change state, Autoloader functions and location references, table, A.5-3
module, 4.43-2 functions of, table, 4.26-1
action to change state, Rotary Cap-Pierce inspection procedure, 4.26-1
module, 4.43-5 list of, by Diluter module, 2.11-2
function and location reference, Autoloader location references, 4.26-1
module, 4.43-2 preventing electronic damage to, 4.26-6
function and location reference, Rotary preventing physical damage to, 4.26-6
Cap-Pierce module, 4.43-5 replacement procedure, 4.26-6
See also sensors, level special headings
shielded reagent lines ATTENTION, 1.1-3
function, 3.3-4 CAUTION, 1.1-3
installation, illustration, 3.3-4 IMPORTANT, 1.1-3
part numbers, 8.1-21 Note, 1.1-3
shipping materials WARNING, 1.1-3
in the Autoloader module, 3.2-5 specifications
in the BSV, 3.2-4 calibration tolerances and limits, A.1-1
in the pinch valves, 3.2-2 electrical input, 3.1-1
in the Pneumatic Power Supply, 3.2-3 electronic noise levels, A.1-2
in the TTM, 3.2-2 performance (background, carryover,
SHUTDOWN PERFORMED PREVIOUSLY, 7.1-25 reproducibility, and mode-to-mode
signal flows, electronic comparison), A.1-2
overviews, 2.3-1 pneumatic tolerances, A.1-5
RF Detector Preamp card, 2.13-6 power input and consumption, A.1-5
See also block diagrams; individual circuit cards pump volumes, A.1-10
Single Ticket Printer. See Printer, Ticket temperature and humidity of
software environment, 3.1-2
avoiding damage to the Sample Handler module timing, A.1-7
during download, 2.7-4 VCS optimization limits, A.1-8
download not successful; WBC, RBC or Plt ventilation requirements, 3.1-2
report 0.00, 7.5-1 voltage ranges, A.1-9
download sequence, 2.4-2 waste drain line length, 3.3-4
download stops after 376 code, 7.5-1 waste drainage, 3.1-3
installation, reference, 3.4-1 specimen tubes
options installation, reference, 3.4-1 stoppers’ pierce proximity adjustment
part numbers, 8.1-21 procedures, 4.41-1
See also software menu trees stoppers’ pierce proximity adjustment,
SOFTWARE AUTOLOADER CHECK FAILED, 7.1-25 illustration, 4.41-1
software menu trees ST1 (sheath tank)
Service Disk 2 utilities, A.6-3 function, A.5-6
software revision 8D and higher for instruments location, illustration, A.5-7
with Autoloader module, A.6-2 Stabilyse (reagent) sensor
software revision 8D and higher for instruments action to change state, 4.43-2
with Rotary Cap-Pierce module, A.6-1 function and location reference, 4.43-2
Solenoid Junction card See also sensors, level
block diagram, 2.4-5 StabiLyse reagent
solenoids conditions causing a PC2 error, 7.2-2
energizing individually or in groups via a service instrument setup procedure, 3.5-1
option, 4.26-1
INDEX
pump. See StabiLyse reagent pump sweep flow. See sweep-flow tank
sensor (FD4). See Stabilyse (reagent) sensor vacuum. See vacuum tank
tubing connection to instrument, TEMP: AMBIENT=XX.XX LYSE=XX.XX, 7.1-27
illustration, 3.3-4 temperature and humidity
StabiLyse reagent pump requirements, 3.1-2
function, A.5-5 temperature sensor, ambient. See ambient
location, illustration, A.5-10 temperature sensor
volume adjustment procedure, 4.16-1 TEST MODE INTERRUPTED, 7.1-27
volume optimization work flow, TICKET PRINTER NOT READY, 7.1-27
illustration, 4.16-3 Ticket Printer. See Printer, Ticket
StabiLyse reagent. See diff leukocyte preservative TKT.CFG FILE I/O ERROR, 7.1-27
stacks. See loading bay; unloading bay tolerances and limits. See specifications
STOP switch tools
action to change state, 4.43-2 and supplies part numbers, 8.1-22
function and location reference, 4.43-2 training, customer
function, type, and location reference, 2.9-2 required before instrument installation, 3.1-1
STOP SWITCH ACTIVATED, 7.1-26 TRANSMIT FAILED - 196 CODE, 7.1-27
supplies TRANSMIT FAILED - 376 CODE, 7.1-27
and tools part numbers, 8.1-22 TRANSMIT FAILED - DILUTER TABLE, 7.1-27
required at installation, 3.1-3 TRANSMIT PORT NOT AVAILABLE, 7.1-27
SVP. See system verification procedure TRANSMIT TO 376 FAILED - 196 CODE, 7.1-27
sweep-flow canister. See sweep-flow tank Triple Transducer module. See TTM
sweep-flow line troubleshooting
function, 2.12-2 count ratio problems, 4.9-3
sweep-flow tank DC, RF, or LS noise, 7.3-1
function, A.5-6 diff or retic latex calibration problems, 7.4-1
location, illustration, A.5-8 electronic noise on the RF channel, table, 7.3-3
switches engineering schematics needed, 6.1-1
checking procedure, 4.43-1 flow-cell errors, 7.2-1
DMS line voltage select. See DMS line voltage laser/flow cell alignment problems, 4.15-1
select switch reported instrument problems, 7.5-1
POWER. See POWER switch TTM
S2-whole blood (whole blood). See whole blood baseplate shipping screw location,
switch illustration, 3.2-2
S4-stop (STOP). See STOP switch component part numbers, illustrated parts
SYSTEM BACKGROUND TIME OUT, 7.1-26 list, 8.2-37, 8.2-39
System Test component part numbers, master parts
not working correctly, 7.5-2 list, 8.1-23
procedure, 3.6-1 location, illustration, 2.1-3
system verification procedure main components, illustration, 2.13-3
form, part number, 5.1-1 shipping materials, removal, 3.2-2
purpose, tools needed, and directions, 5.1-1 tube available sensor
systems action to change state, Autoloader module
instrument, list of, 2.1-4 (S7), 4.43-3
action to change state, Rotary Cap-Pierce
module (U5), 4.43-6
T function and location reference, Autoloader
tanks module (S7), 4.43-3
pressure. See pressure tank function and location reference, Rotary
sheath. See sheath tank Cap-Pierce module (U5), 4.43-6
INDEX
function, type, and location reference, UNIDENTIFIED S44 ERROR, 7.1-28

Autoloader module (S7), 2.9-2 UNIDENTIFIED S45 ERROR, 7.1-28
function, type, and location reference, Rotary UNIDENTIFIED S55 ERROR, 7.1-29
Cap-Pierce module (U5), 2.8-2 UNIDENTIFIED S57 ERROR, 7.1-29
location, illustration, Autoloader module unload elevator
(S7), 2.9-3 location, illustration, 2.9-3
location, illustration, Rotary Cap-Pierce module unload elevator down sensor
(U5), 2.8-2 action to change state, 4.43-3
replacement procedure, 4.37-1 function and location reference, 4.43-3
TUBE AVAILABLE SENSOR ERROR, 7.1-27 function, type, and location reference, 2.9-2
tube ejector and tray location, illustration, 2.9-3
function, 2.8-1 UNLOAD ELEVATOR FAILURE, 7.1-29
location, illustration, 2.8-2 unload elevator motor
tube forward sensor function, type, and location reference, 2.9-1
action to change state, 4.43-3 location, illustration, 2.9-4
function and location reference, 4.43-3 replacement procedure, 4.36-1
function, type, and location UNLOAD STACK FULL, 7.1-29
reference, 2.9-3, 2.9-4 unload stack full sensor
tube ram cylinder action to change state, 4.43-2
location, illustration, 2.9-4 function and location reference, 4.43-2
tube ram sensor function, type, and location reference, 2.9-3
action to change state, 4.43-3 location, illustration, 2.9-3, 2.9-4
function and location reference, 4.43-3 unload stack. See unloading bay
function, type, and location reference, 2.9-3 unloading bay
location, illustration, 2.9-4 function, 2.9-1
tube return cylinder location, illustration, 2.9-3, 2.9-4
location, illustration, 2.9-4 upgrades and options kits
tubes part numbers, 8.1-16
pickup and sensors, part numbers, 8.1-16
specimen. See specimen tubes
tubing V
color coding used in Diluter, 2.11-2 V (volume or DC)
part numbers, 8.1-25 troubleshooting latex calibration
reagent input, illustration, 3.3-4 problems, 7.4-1
shielded reagent, part numbers, 8.1-21 VCS measurement, description, 2.13-1
vacuum
high. See high vacuum
U low. See low vacuum
U1 (needle home sensor). See needle home sensor vacuum chamber
U2 (needle forward sensor). See needle forward function, A.5-6
sensor location, illustration, A.5-10
U3 (normal position sensor). See normal position vacuum tank
sensor function, A.5-6
U4 (oversized pos. sensor). See oversized pos.sensor location, illustration, 2.5-2
U5 (tube available sensor). See tube available sensor valves
UNABLE TO OPEN/READ 196CODE.HEX, 7.1-28 pinch. See pinch valves
UNABLE TO OPEN/READ 376CODE.HEX, 7.1-28 solenoids. See solenoids
UNABLE TO OPEN/READ DILUTER TBL, 7.1-28 VC1 (waste chamber)
UNABLE TO PROCESS REQUEST, 7.1-28 function, A.5-6
UNIDENTIFIED ERROR [XXXX], 7.1-28 location, illustration, A.5-8
INDEX
VC2 (bubble trap) checking RMS noise levels on the DC, RF, and LS
function, A.5-6 channels, 4.12-1
location, illustration, A.5-8 checking the count ratio, 4.9-3
VC3 (vent waste chamber) cleaning the flow cell, 4.13-1
function, A.5-6 making preliminary checks, 4.9-3
location, illustration, A.5-11, A.5-12 procedure flow, illustration, 4.9-1
VC4 (needle vent chamber) procedures, overview, 4.9-1
function, A.5-6 verifying successful optimization, 4.9-4
location, illustration, A.5-11, A.5-12 See also calibration, VCS
VC5 (bleach chamber) VCS PROCESSOR card
function, A.5-6 block diagram, 2.4-8
location, illustration, A.5-10 component locations, illustration, A.2-16
VC6 (vacuum chamber) function, 2.4-8
function, A.5-6 inputs/outputs, 2.4-9
location, illustration, A.5-10 jumper setting, A.2-16
VC7 (diff waste chamber) location, illustration, 2.4-1
function, A.5-6 test point function, table, A.2-16
location, illustration, A.5-10 V, C, S test points location/function, 2.4-9
VC8 (overflow chamber) VCS technology
function, A.5-6 applying, 2.13-2
location, illustration, A.5-8 DC and RF currents, 2.13-2
VC9 (vacuum tank) flow-cell hydraulics, 2.13-3
function, A.5-6 flow-cell ports and sample and reagent flow,
location, illustration, 2.5-2 illustration, 2.13-4
VC10 (RBC vacuum isolator chamber) hydrodynamically focused cells,
function, A.5-6 illustration, 2.13-4
location, illustration, A.5-8 laser light source, 2.13-2
VC11 (WBC vacuum isolator chamber) light scatter path, illustration, 2.13-3
function, A.5-6 measurements obtained, 2.13-1
location, illustration, A.5-8 parameters reported, 2.1-1, 2.13-1
VCS calibration. See calibration, VCS; VCS sensing area, illustration, 2.13-3
optimization temperature control of Erythrolyse II
VCS flow rate reagent, 2.6-4
adjusting at instrument installation, 3.7-2 temperature range cycles, 2.6-4
adjustment procedure, 4.10-1 temperature range cycles, table of, 2.6-5
VCS measurements test points for measuring noise levels, 2.4-9
conductivity (C or RF), 2.13-1 See also calibration, VCS; VCS optimization
linear light scatter (LLS), 2.13-2 vent waste chamber
opacity (OP), 2.13-2 function, A.5-6
rotated light scatter (RLS), 2.13-2 location, illustration, A.5-11, A.5-12
volume (V or DC), 2.13-1 ventilation
VCS optimization instrument requirements, 3.1-2
adjusting C1 on the RF Detector card, 4.11-2 VIP. See system verification procedure
adjusting the Clog Detector circuit, 4.11-1 voltages
adjusting the DC, RF, and LS gains, 4.4-2 blood/bubble detector cables, acceptable
adjusting the Erythrolyse II reagent pump and ranges, A.1-9
StabiLyse reagent pump volumes, 4.16-1 checks and adjustments, 4.23-1
adjusting the VCS flow rate, 4.10-1 equivalents for pneumatic readings, table, A.2-8
aligning the laser/flow cell, 4.15-1 Hgb blank, acceptable ranges, A.1-9
system, acceptable ranges, A.1-9
INDEX
volume (V or DC) WBC bath

VCS measurement, description, 2.13-1 function, A.5-2
voting criteria location, illustration, A.5-8
MCV, RDW, Plt and MPV, 2.4-13 replacement, 4.28-1
RBC and WBC, 2.4-10 See also Aperture module and bath assembly
WBC BATH OVERFLOW, 7.1-30
WBC diluent dispenser
W function, A.5-5
WARNING location, illustration, A.5-10
heading defined, 1.1-3 measuring volume dispensed by volume, 4.19-1
waste measuring volume dispensed by weight, 4.19-2
container, neck support bracket installed, 3.3-5 volume adjustment procedure, 4.19-1
drain line connection to instrument, volume tolerances, A.1-10
illustration, 3.3-4 WBC vacuum isolator chamber
drain line connections, 3.3-4 function, A.5-6
drainage requirements, 3.1-3 location, illustration, A.5-8
instrument setup procedure, 3.5-1 WHITE A/I/V OUT OF RANGE, 7.1-30
level sensing, 2.6-5 white blood cell count. See WBC
removal, 2.6-5 white blood cell. See WBC
sensor. See waste sensor whole blood switch
specifications for drain line length, 3.3-4 action to change state, Autoloader
waste chamber module, 4.43-1
function, A.5-6 action to change state, Rotary Cap-Pierce
location, illustration, A.5-8 module, 4.43-5
WASTE CONTAINER FULL, 7.1-30 function and location reference, Autoloader
waste sensor module, 4.43-1
action to change state, Autoloader function and location reference, Rotary
module, 4.43-1 Cap-Pierce module, 4.43-5
action to change state, Rotary Cap-Pierce WORKLIST ERRORS NOT CLEARED, 7.1-30
module, 4.43-5 WORKLIST FULL, 7.1-30
function and location reference, Autoloader WRONG ASSAY SHEET IN USE, 7.1-30
module, 4.43-1 WRONG DIGIBOARD SOFTWARE, 7.1-31
function and location reference, Rotary WRPORT UNAVAIL FOR 376 CODE, 7.1-31
Cap-Pierce module, 4.43-5
See also sensors, level
WATER TRAP DID NOT BLEED [XX.XX], 7.1-30
WBC
checking carryover in the Primary mode, 4.6-1
counting sequences, 2.4-10
histogram development, 2.4-10
histogram particle accumulation, 2.4-10
method used for determining, 2.12-1
reports 0.00; software download not
successful, 7.5-1
test point for measuring noise level, 2.4-13
verifying reproducibility in the Primary
mode, 4.5-1
verifying reproducibility in the Secondary
mode, 4.5-2
voting criteria, 2.4-10
TRADEMARKS
The BECKMAN COULTER logo, 4C PLUS, 5C, ACCUVETTE, COULTER, COULTER

CLENZ, Erythrolyse, ISOTON, LATRON, LYSE S, MAXM, RETIC-C, ReticPrep, S-CAL,
SCATTER PAK, and StabiLyse are trademarks of Beckman Couter, Inc.
All other trademarks, service marks, products, or services are trademarks or registered
trademarks of their respective holders.
PN 4235961E

Service Manual Beckman Coulter Maxm

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COULTER® MAXM™ Hematology Analyzer

COULTER® MAXM™ Hematology Analyzer

PN 4235961E (August 2000)

HAZARDS AND OPERATIONAL PRECAUTIONS AND LIMITATIONS

WARNINGS, CAUTIONS, and IMPORTANTS alert you as follows:

WARNING - Might cause injury.

Copyright © Beckman Coulter, Inc. 1992-2000

Initial Issue, 8/92

REVISION STATUS, iii

1.1 MANUAL DESCRIPTION, 1.1-1

1.2 SAFETY PRECAUTIONS, 1.2-1

2 INSTRUMENT DESCRIPTION, 2.1-1

2.1 INTRODUCTION TO THE COULTER MAXM ANALYZER, 2.1-1

2.2 ELECTRONIC SYSTEM - OVERVIEW AND POWER SUPPLIES, 2.2-1

Laser Power Supply, 2.2-4

2.3 ELECTRONIC SYSTEM - SIGNAL FLOW BLOCK DIAGRAMS, 2.3-1

2.4 ELECTRONIC SYSTEM - ANALYZER MODULE, 2.4-1

2.5 PNEUMATIC SYSTEM, 2.5-1

2.6 REAGENT SYSTEM, 2.6-1

2.7 SAMPLE HANDLER SYSTEM - SAMPLE HANDLER CARD, 2.7-1

2.8 SAMPLE HANDLER SYSTEM - ROTARY CAP-PIERCE MODULE, 2.8-1

2.9 SAMPLE HANDLER SYSTEM - AUTOLOADER MODULE, 2.9-1

Summary of Operation, 2.9-5

2.10 SAMPLE HANDLER SYSTEM - AUTOSENSOR TEST CARD, 2.10-1

2.11 SAMPLE PROCESSING SYSTEM - DILUTER, 2.11-1

2.12 SAMPLE ANALYSIS SYSTEM - CBC TECHNOLOGY, 2.12-1

2.13 SAMPLE ANALYSIS SYSTEM - VCS TECHNOLOGY, 2.13-1

Flow-Cell Hydraulics, 2.13-3

3 INSTALLATION PROCEDURES, 3.1-1

PART A: INSTRUMENT INSTALLATION

3.1 PREINSTALLATION CHECK, 3.1-1

3.2 INITIAL SETUP, 3.2-1

3.3 CONNECTING ASSEMBLIES, 3.3-1

3.4 TESTING/CONFIGURING ASSEMBLIES, 3.4-1

3.5 INITIAL SYSTEM SETUP, 3.5-1

3.6 SYSTEM TESTING, 3.6-1

Verifying General Operation of the Primary Mode on Instruments with a Rotary

3.7 ADJUSTMENTS AND CALIBRATION, 3.7-1

3.8 ACCOUNT/INSTRUMENT INFORMATION, 3.8-1

S. S-CAL Calibrator Calibration Batch Tables/Cal Factors, 3.8-18

PART B: UPGRADES AND OPTIONS INSTALLATION

3.9 Setting Up the Optional Printers, 3.9-1

3.10 CONFIGURING THE MAXM ANALYZER FOR CYANIDE-FREE

4 SERVICE AND REPAIR PROCEDURES, 4.1-1

4.1 GUIDELINES FOR SERVICING THE MAXM ANALYZER, 4.1-1

4.2 USING THE SERVICE DISK, 4.2-1

4.3 WHOLE BLOOD VERIFICATION - CBC/DIFF, 4.3-1

4.4 LATEX CALIBRATION AND VERIFICATION, 4.4-1

4.5 REPRODUCIBILITY CHECKS, 4.5-1

4.6 CARRYOVER CHECKS, 4.6-1

4.7 INITIAL ADJUSTMENT OF PRIMARY-MODE CALIBRATION FACTORS, 4.7-1

4.8 SECONDARY MODE-TO-PRIMARY MODE CALIBRATION, 4.8-1

4.9 VCS OPTIMIZATION, 4.9-1

D. Clog Detector Circuit Adjustment, 4.9-4

4.10 VCS FLOW-RATE ADJUSTMENT, 4.10-1

4.11 RF DETECTOR PREAMP CARD ADJUSTMENTS, 4.11-1

4.12 RMS NOISE CHECKS, 4.12-1

4.13 FLOW-CELL CLEANING, 4.13-1

4.14 LENS BLOCK CLEANING, 4.14-1

4.15 LASER/FLOW CELL ALIGNMENT, 4.15-1