DW

0 hrs
10^5 10^6 10^7 Cfu/mL Average Standard deviation
R1 25 2 0 26000000 2.8 x 10^7 1527525.23165
28 1 0
R2 30 1 0 29000000
28 1 0
R3 25 3 0 28000000
30 3 0
cfu/mL +/- SD 2.8 x 10^7 +/- 1.5 x10^6
DW-0.85% NaCl
0 hrs
10^5 10^6 10^7 Cfu/mL Average Standard deviation
R1 300 35 3 320000000 3.4 X 10^8 20816659.9947
302 30 3
R2 310 36 3 360000000
307 35 2
R3 301 30 3 350000000
285 40 1
cfu/mL +/- SD 3.4 x 10^8 +/- 2.1 X 10^7
DW-3% NaCl
0 hrs
10^5 10^6 10^7 Calculation Cfu/mL Average
375 40 3 380000000
R1 390 37 4
380 37 2 380000000
R2 398 40 3
355 35 4 320000000
R3 365 28 10 360000000
cfu/mL +/- SD 3.6 x 10^8 +/- 3.5 X 10^7
PBS
0 hrs
10^5 10^6 10^7 Computation Cfu/mL Average
320 30 2
R1 333 27 1 280000000
321 27 1
R2 332 34 2 310000000
319 33 3
R3 325 31 3 320000000 303333333.333
cfu/mL +/- SD 3.0 x 10^8 +/- 2.1 X 10^7
PBS - 0.85 % NaCl
0 hrs
10^5 10^6 10^7 Calculation Cfu/mL Average
320 32 1
R1 330 35 3 340000000
330 35 3
R2 350 33 1 340000000
310 36 1
R3 345 34 0 350000000 343333333.333
cfu/mL +/- SD 3.4 x 10^8 +/- 5.8 X 10^7
PBS - 3.0 % NaCl
0 hrs
10^5 10^6 10^7 Calculation Cfu/mL Average
R1 380 42 5
390 41 4 420000000
R2 390 45 4
400 47 4 460000000
R3 395 39 4
431 43 1 410000000 430000000
cfu/mL +/- SD 4.3 x 10^8 +/- 2.6X 10^7

#NAME?

Dilution cfu/mL Absorbance

1 0 0 0
2 0.02 500000000 0.05 Stand
3 0.04 1000000000 0.08 10 60
4 0.06 1500000000 0.15 15
50
5 0.08 2000000000 0.2 20 f(x) = 98.5757663389242 x + 1.3
R² = 0.995615240023135
cells per mL(10^8)

6 0.1 2500000000 0.24 25 40

7 0.12 3000000000 0.29 30
30
8 0.14 3500000000 0.33 35
9 0.16 4000000000 0.38 40 20
10 0.18 4500000000 0.45 45
10
11 0.2 5000000000 0.5 50
0
0.05 0.1 0.15 0.2 0.25
 2 hrs
ard deviation 10^5 10^6 10^7 Cfu/mL Average Standard deviation
R1 0 0 0 0 0 0
0 0 0
R2 0 0 0 0
0 0 0
R3 0 0 0 0
0 0 0
cfu/mL +/- SD 0

2 hrs
ard deviation 10^5 10^6 10^7 Cfu/mL Average Standard deviation
R1 251 25 2 240000000 243333333.3333 5773502.691896
240 28 1
R2 245 25 1 250000000
250 27 0
R3 237 30 2 240000000
265 22 2
cfu/mL +/- SD 2.4 x 10^8 +/- 5.8X 10^6

2 hrs
Standard deviation 10^5 10^6 10^7 Calculation Cfu/mL Average
350 30 3
R1 325 35 3 320000000
345 35 2
R2 333 30 5 320000000
360 33 3
34641016.151 R3 350 37 1 350000000 330000000
cfu/mL +/- SD 3.3 x 10^8 +/- 1.7 X 10^7

2 hrs
Standard deviation 10^5 10^6 10^7 Computation Cfu/mL Average
255 25 2
R1 245 24 2 240000000
230 26 2
R2 245 27 2 240000000
242 28 2
20816659.995 R3 250 29 1 250000000 243333333.3333
 cfu/mL +/- SD 2.5 x 10^8 +/- 5.8 X 10^6

2 hrs
Standard deviation 10^5 10^6 10^7 Calculation Cfu/mL Average
245 27 1
R1 249 25 1 250000000
245 24 2
R2 245 25 2 240000000
250 25 1
5773502.6919 R3 252 27 1 250000000 246666666.6667
cfu/mL +/- SD 2.5 x 10^8 +/- 5.8 X 10^6

2 hrs
Standard deviation 10^5 10^6 10^7 Calculation Cfu/mL Average
375 40 4
R1 350 42 4 410000000
365 36 1
R2 375 37 1 360000000
350 40 4
26457513.111 R3 365 37 0 380000000 383333333.3333
cfu/mL +/- SD 3.8 x 10^8 +/- 2.5 X 10^7

Standard Curve
60

50
f(x) = 98.5757663389242 x + 1.3034991324465
R² = 0.995615240023135
40

30

20

10

0
0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45 0.5 0.55
Absrorbance
 Mean percent change in population DW DW-0.85% NaDW-3% NaCl PBS
rd deviation -1% 0h 2.8 x 10^ 3.4 x 10^8 cfu 3.6 x 10^83.0 x 10^8 cfu/ mL
2h 0 2.4 x 10^8 cf 3.3 x 10^8 cfu2.5 x 10^8
percent change in population

Log mean count

28000000 7.447158 340000000 8.531478917 3.6E+08
0 240000000 8.380211242 3.3E+08

-29%
rd deviation

Standard deviation 8.30%

17320508.0757

Standard deviation
-16.67

5773502.6919
Standard deviation
-26.47%

5773502.6919

Standard deviation
-11.63%

25166114.7842
 PBS - 0.85 PBS - 3.0 % NaCl
3.4 x 10^8 cfu/mL
4.3 x 10^8 cfu/m
2.5 x 10^8 3.8 x 10^8 cfu/mL

8.556302 300000000 8.477121 340000000 8.531479 430000000 8.633468

8.518514 250000000 8.39794 250000000 8.39794 380000000 8.579784