HCT Laboratory 1

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HCT LABORATORY – EXERCISE 1
Histopathologic and Cytologic Technique (HCT) was formerly known as GPHCT.
HISTOTECHNOLOGIST – process the tissue specimen from the surgical room to the histopathology laboratory in
preparation for the diagnosis of any malignancy.
- Not allowed to interpret the result since it is the role of the PATHOLOGIST.
BLOCK NO. TISSUE STAIN
1 LIVER – 10 slides Hematoxylin & Eosin
2 KIDNEY – 10 slides Masson’s Trichrome
3 LUNG – 5 slides Fite Faraco
- 5 slides Kinyoun
MATERIALS
TEASING NEEDLE- blunt end is useful for tissue processing. The needle of the syringe can also be used.
Barbecue stick attached to the

port of the syringe needle
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HAIRBRUSH – a good hairbrush has soft bristle. DO NOT USE MAKE-UP BRUSH.
SCALPEL WITH HANDLE (Metal or Plastic) MEDICINE DROPPER (Glass or plastic)
24 Gauge
Scalpel Blade
Scalpel Handle
MEYER’S EGG ALBUMIN

primarily used as an adhesive
in order for the tissue section
to adhere or stick to the glass
slides.
¼ size illustration board with
white and black sides
Things to consider in tissue processing:
- Observe proper removal of Bubbles and

Proper Alignment of folds
tissue section - Proper Alignment of tissue section
- Cleanliness of slides
- Thickness during section cutting
- Effect of Staining processes
Clean, free of excess wax, stains

and fingerprints
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Tissue Processing Steps
1. Fixation
2. Dehydration
3. Clearing
4. Wax Infiltration or wax imprintation
Situation: A kidney is brought to the laboratory for gross examination by the PATHOLOGIST. Once the specimen is done
with gross examination, it will be placed in a tissue cassette.
Tissue Cassette or Tissue Receptacle (Vintage type)
Specimen thinly cut by

Pathologist
1. The HISTOTECHNOLOGIST will prepare the LABEL following the APPROPRIATE SIZE ordered by the PATHOLOGIST.
Note: Use
PENCIL, not
ballpen because
the name will be
1st Name Family name smudged.
Electric Paraffin Pitcher or

Electric Paraffin Wax
Dispenser Paraffin Wax
(inside) Melted Paraffin Wax

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2. Fold the name and place it in the tissue cassette together with the
specimen.
3. Cassette will be placed in a basket with solution. Purpose of holes is to
allow the penetration of solution (fixative, dehydrating or clearing agent).
4. Assuming it has been processed by the 4 processing steps, it will result

to a TISSUE BLOCK.
5. Embed the tissue block in the PARAFFIN BLOCK.
Tissue
Block Paraffin Block
6. Place the Melted paraffin into the Hinge pop-out mold and embed the
tissue block at the center. Should be properly aligned and parallel to the hinge
embedding mold and allow it to harden or solidify.
Hinge Pop-out 7. Place paraffin block on ICE to hasten solidification and to prevent
mold crystallization of paraffin block. It should not exceed 5 minutes.
8. Excess wax can be removed using the Manual trimmer or scalpel.

Manual Trimming helps us in the proper sectioning and ribboning of our tissue section.
Trimmer Make sure to not hit the tissue section.
Note: Do not touch the TISSUE with your bare hands.
Electric Electric
Paraffin Bath Paraffin Knife
It will be used after
embedding. Can also
be used to affixed
the label.
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Paraffin Block
Holder
Rotary
Blade
Microtome
You can rotate the ROTARY MICROTOME in a CONTINUOUS CLOCKWISE manner
9. To know how thick or thin the tissue section has been

cut. Set the gauge to the ideal thickness which is 5 micra.
10. Picture on the left shows the thin ribbons to be used for mounting. You
have to separate it one-by-one very carefully and mount it on the glass slide by
using the Meyer’s egg albumin as adhesive.
Use SCALPEL to separate the ribbons.
Tissue Flotation
11. There is a designated temperature to help flattened the tissue sections
Bath
and to prevent the formation of folds.
12. Tissue sections that have been embedded, trimmed, cut thinly and
mounted to the slide, next is to proceed to STAINING. Before staining, clean
the excess wax.

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@gwinniemarie

HCT LABORATORY – EXERCISE 1

Histopathologic and Cytologic Technique (HCT) was formerly known as GPHCT.

Barbecue stick attached to the

SCALPEL WITH HANDLE (Metal or Plastic) MEDICINE DROPPER (Glass or plastic)

MEYER’S EGG ALBUMIN

Things to consider in tissue processing:

- Observe proper removal of Bubbles and

Clean, free of excess wax, stains

4. Wax Infiltration or wax imprintation

Tissue Cassette or Tissue Receptacle (Vintage type)

Specimen thinly cut by

Electric Paraffin Pitcher or

(inside) Melted Paraffin Wax

4. Assuming it has been processed by the 4 processing steps, it will result

8. Excess wax can be removed using the Manual trimmer or scalpel.

Note: Do not touch the TISSUE with your bare hands.

You can rotate the ROTARY MICROTOME in a CONTINUOUS CLOCKWISE manner

9. To know how thick or thin the tissue section has been

Use SCALPEL to separate the ribbons.

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