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Gat B Syllabus

The Graduate Aptitude Test-Biotechnology (GAT-B) 2020 will be a 180-minute exam with two parts. Part A will contain 60 multiple choice questions testing 10+12 level biology, chemistry, physics, and mathematics. Part B will contain 100 multiple choice questions testing bachelor's level biology, life sciences, biotechnology, and related areas; candidates must attempt 60 questions. The exam syllabus covers topics such as biomolecules, microbiology, genetics, immunology, bioinformatics, bioprocess engineering, plant biotechnology, animal cell culture, and recombinant DNA technology.

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137 views3 pages

Gat B Syllabus

The Graduate Aptitude Test-Biotechnology (GAT-B) 2020 will be a 180-minute exam with two parts. Part A will contain 60 multiple choice questions testing 10+12 level biology, chemistry, physics, and mathematics. Part B will contain 100 multiple choice questions testing bachelor's level biology, life sciences, biotechnology, and related areas; candidates must attempt 60 questions. The exam syllabus covers topics such as biomolecules, microbiology, genetics, immunology, bioinformatics, bioprocess engineering, plant biotechnology, animal cell culture, and recombinant DNA technology.

Uploaded by

DIKCHHA AGRAWAL
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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GRADUATE APTITUDE TEST-BIOTECHNOLOGY (GAT-B) 2020

The examination will be conducted in a single shift for a duration of 180 minutes. The question
paper will have two parts:

PART- A: Part A will have 60 compulsory multiple choice questions of the level of 10+2 in the
subjects: Physics, Chemistry, Mathematics and Biology. Each correct answer will be of one
mark each. There will be negative marking and for each wrong answer, ½ (half) mark will be
deducted.

PART-B: Part B will have multiple choice questions of bachelor’s level requiring thinking and
analysis. There will be questions from basic Biology, Life Sciences, Biotechnology and allied
areas as per syllabus given below. There will be 100 questions out of which the candidates
will have to attempt 60 questions. Each correct answer will have a weightage of 3 marks.
There will be negative marking and for each wrong answer one mark will be deducted.

PART-B SYLLABUS

Biomolecules-structure and functions; Biological membranes, structure, action potential


and transport processes; Enzymes- classification, kinetics and mechanism of action; Basic
concepts and designs of metabolism (carbohydrates, lipids, amino acids and nucleic acids)
photosynthesis, respiration and electron transport chain; Bioenergetics.

Viruses- structure and classification; Microbial classification and diversity(bacterial, algal and
fungal); Methods in microbiology; Microbial growth and nutrition; Aerobic and
anaerobic respiration; Nitrogen fixation; Microbial diseases and host-pathogen interaction.

Prokaryotic and eukaryotic cell structure; Cell cycle and cell growth control; Cell-Cell
communication, Cell signaling and signal transduction.

Molecular structure of genes and chromosomes; Mutations and mutagenesis; Nucleic acid
replication, transcription, translation and their regulatory mechanisms in prokaryotes and
eukaryotes; Mendelian inheritance; Gene interaction; Complementation; Linkage,
recombination and chromosome mapping; Extra chromosomal inheritance; Microbial
genetics (plasmids, transformation, transduction, conjugation); Horizontal gene transfer and
Transposable elements; RNA interference; DNA damage and repair; Chromosomal variation;
Molecular basis of genetic diseases.

Principles of microscopy-light, electron, fluorescent and confocal; Centrifugation- high


speed and ultra; Principles of spectroscopy-UV, visible, CD, IR, FTIR, Raman, MS,NMR;
Principles of chromatography- ion exchange, gel filtration, hydrophobic interaction, affinity,
GC,HPLC, FPLC; Electrophoresis; Microarray.

History of Immunology; Innate, humoral and cell mediated immunity; Antigen; Antibody
structure and function; Molecular basis of antibody diversity; Synthesis of antibody and
secretion; Antigen-antibody reaction; Complement; Primary and secondary lymphoid organ;
B and T cells and macrophages; Major histocompatibility complex (MHC); Antigen
processing and presentation; Polyclonal and monoclonal antibody; Regulation of immune
response; Immune tolerance; Hypersensitivity; Autoimmunity; Graft versus host reaction.

Major bioinformatics resources and search tools; Sequence and structure databases;
Sequence analysis (biomolecular sequence file formats, scoring matrices, sequence
alignment, phylogeny); Data mining and analytical tools for genomic and proteomic studies;
Molecular dynamics and simulations (basic concepts including force fields, protein-protein,
protein-nucleic acid, protein- ligand interaction)

Restriction and modification enzymes; Vectors; plasmid, bacteriophage and other viral
vectors, cosmids, Ti plasmid, yeast artificial chromosome; mammalian and plant expression
vectors; cDNA and genomic DNA library; Gene isolation, cloning and expression ;
Transposons and gene targeting; DNA labeling; DNA sequencing; Polymerase chain
reactions; DNA fingerprinting; Southern and northern blotting; In- situ hybridization; RAPD,
RFLP; Site-directed mutagenesis; Gene transfer technologies; Gene therapy.

Totipotency; Regeneration of plants; Plant growth regulators and elicitors; Tissue culture
and Cell suspension culture system: methodology, kinetics of growth and, nutrient
optimization; Production of secondary metabolites by plant suspension cultures; Hairy root
culture; transgenic plants; Plant products of industrial importance.

Animal cell culture; media composition and growth conditions; Animal cell and tissue
preservation; Anchorage and non-anchorage dependent cell culture; Kinetics of cell
growth; Micro & macro-carrier culture; Hybridoma technology; Stem cell technology; Animal
cloning; Transgenic animals.

Chemical engineering principles applied to biological system, Principle of reactor design,


ideal and non- ideal multiphase bioreactors, mass and heat transfer; Rheology of
fermentation fluids, Aeration and agitation; Media formulation and optimization; Kinetics of
microbial growth, substrate utilization and product formation; Sterilization of air and media;
Batch, fed-batch and continuous processes; Various types of microbial and enzyme
reactors; Instrumentation control and optimization; Unit operations in solid-liquid separation
and liquid-liquid extraction; Process scale-up, economics and feasibility analysis.

Engineering principle of bioprocessing - Upstream production and downstream; Bioprocess


design and development from lab to industrial scale; Microbial, animal and plant cell culture
platforms; Production of biomass and primary/secondary metabolites; Biofuels, Bioplastics,
industrial enzymes, antibiotics; Large scale production and purification of recombinant
proteins; Industrial application of chromatographic and membrane based bio-separation
methods; Immobilization of biocatalysts (enzymes and cells) for bioconversion processes;
Bioremediation-Aerobic and anaerobic processes for stabilization of solid / liquid wastes.

Tissue culture and its application, Micropropagation. Meristem culture and production
of virus-free plants. Anther and microspore culture. Embryo and ovary culture.
Protoplast isolation. Protoplast fusion-somatic hybrids, cybrids. Somaclones.
Synthetic seeds. In vitro germplasm conservation. Cryopreservation. Organelle DNA,
Satellite-and repetitive DNAs. DNA repair. Regulation of gene expression.
Recombinant DNA technology-cloning vectors, restriction enzymes, gene cloning.
Methods of gene transfer in plants. Achievements and recent developments of genetic
engineering in agriculture. Development of transgenics for biotic & abiotic stress
tolerance, bioethics, terminator technology, nanotechnology, DNA fingerprinting, gene
silencing.

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