Analisis kuantitatif sediaan

obat multikomponen dengan

spektrofluorometri

Preparasi untuk analisis sediaan obat

multikomponen
 Tahapan analisis kuantitatif obat
multikomponen dalam sediaan obat
padat, semi padat, cair dan steril secara
spektrofluorometri
M. SULAIMAN ZUBAIR
(www.abuumar80.wordpress.com)
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Istilah
y Analisis
kuantitatif : analisis penentuan kadar suatu
senyawa didalam suatu sampel
y Sediaan obat : padat (tablet, kapsul), semipadat (salep),
cair (sirup, emulsi, suspensi) dan steril (injeksi)
y Multikomponen : sediaan obat yang terdiri dari beberapa
senyawa aktif obat, seperti kombinasi GG dan
Difenhidramin dalam sirup obat batuk
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Preparasi sediaan obat multikomponen untuk

analisis kuantitatif dengan spektrofluorometri
- Sampel tablet yang akan dianalisis harus
representatif untuk menghindari resiko
adanya hasil analisis yang keluar dari
spesifikasi yang ditentukan. Contoh : Menurut
Farmakope, untuk analisis tablet parasetamol
dibutuhkan sampel sebanyak 20 tablet
parasetamol 500 mg
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Preparasi sediaan obat multikomponen untuk

analisis kuantitatif dengan spektrofluorometri
y Sediaan cair : dapat dilakukan
pengukuran secara langsung, atau
diencerkan atau dipekatkan terlebih
dahulu dengan pelarut organik
y Sediaan steril (injeksi) : dapat
dilakukan pengukuran secara
langsung
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Preparasi sediaan obat multikomponen untuk

analisis kuantitatif dengan spektrofluorometri
ySediaan semi padat
Isolasi obat dalam salep harus ditunjukkan pada
dasar salepnya :
- Salep lemak bulu domba alkohol, biasanya dilarutkan
dalam kloroform atau eter
- Salep hidrofil, dilarutkan dalam kloroform atau eter
- Salep lanolin, dilarutkan dalam kloroform atau eter
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y Pada prinsipnya, semua pengukuran

dengan menggunakan instrumen
spektroskopi, maka syarat pertama
adalah harus larut dalam larutan
pembawa yang digunakan
y Meskipun sediaan itu merupakan sediaan
obat multikomponen, maka dengan
instrumen spektroskopi maupun
kromatografi dapat membedakan masing-
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Tinjauan
Umum
Spektrofluorometri
y Spektrofotometri fluoresensi merupakan suatu
prosedur yang menggunakan pengukuran intensitas
cahaya fluoresensi yang dipancarkan oleh zat uji
dibandingkan
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Instrumentasi

y Pengukuran intensitas fluoresensi dapat

dilakukan dengan suatu fluorometer
filter
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Fluoresensi dan Struktur Molekul

y Supaya terjadi fluoresensi, harus terjadi peresapan
cahaya yang kuat oleh suatu molekul. Hal ini
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H
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kelompok analisis obat secara

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kelompok analisis obat secara

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b. Pengolahan Data
4. Larutan
Pengenceran standar : 0,2 ppm
Larutan awal : 100 ppm V1.M1 = V2.M2
1. Dibuat Larutan Stok : V1.1 ppm = 25
1 ppm mL. 0,2ppm
V1 = 5 mL
Perhitungan
5. Larutan
V1.M1 =
Standar : 0,25 ppm
V2.M2 V1.M1 = V2.M2
V1.100 ppm = 100 mL. V1.1 ppm = 25
1ppm V1 = 1 mL mL. 0,25ppm
2. Larutan standar : 0,1 ppm V1 = 6,25 mL
V1.M1 = V2.M2 6. Larutan Standar :
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y Tabel 2. Pengolahan Data

Bahan Intensitas pada =  Intensitas Bahan-Blanko

450
nm pada =  450nm

Blanko 0,4 -
Sampel 31 30,6
0,1 pp m 14 13,6

0,15 pp m 19.9 19,5

0,2 ppm 26 25,6

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y Dari grafik didapatkan persamaan regresi:

y = bx + a
I = m.C + Io
I = 124,2 C + 0,94
Dengan perhitungan kalkulator didapat R2 = 1
Maka dapat diketahui konsentrasi sampel dengan cara substitusi
nilai intensitas sampel dikurangi blanko pada panjang gelombang
450nm. I sampel = 30,6
I = 124,2 C +
0,94 I = 124,2 C
+ 0,94
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Sekian dan
Terimakasih