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Characteristics of Normal Cells Vs Transformed Cells: Ayman Saeed 2020-MPHIL-1243

Normal cells are non-malignant, non-invasive, and do not have activated oncogenes or their products present. Transformed cells are malignant, invasive, and have activated oncogenes and their products present. The characteristics of normal vs transformed cells can be tested through assays such as cell transformation assays, DNA extraction and hybridization, karyotyping, microscopy, immunofluorescent staining, RNA microarrays, and antibody testing.

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Ujala Asad
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47 views

Characteristics of Normal Cells Vs Transformed Cells: Ayman Saeed 2020-MPHIL-1243

Normal cells are non-malignant, non-invasive, and do not have activated oncogenes or their products present. Transformed cells are malignant, invasive, and have activated oncogenes and their products present. The characteristics of normal vs transformed cells can be tested through assays such as cell transformation assays, DNA extraction and hybridization, karyotyping, microscopy, immunofluorescent staining, RNA microarrays, and antibody testing.

Uploaded by

Ujala Asad
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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CHARACTERISTICS OF NORMAL CELLS VS

TRANSFORMED CELLS
AYMAN SAEED
2020-MPHIL-1243

SUBMITTED TO
PROF. Dr MASOOD RABBANI
CHARACTERISTCS NORMAL CELL TRANSFORMED CELL TESTING

Neoplastic Non malignant Malignant Cell transformation assay


Non invasive Invasive
Protease secretion

Oncogenes Not activated Activated Extract DNA from tumor


cell, break into fragments,
introduce into fibroblasts
in cell culture. If it
contains any oncogene,
small colonies of
proliferating cells form.
2nd method is DNA
hybridization technique.
Products of oncogenes Absent Present By
radioimmunoprecipitation

Chromosome number Normal Abnormal By karyotyping

Morphology Shape, size, growth Cells become refractile, Under inverted


pattern shape change, microscope
rounding, growth in
irregular patterns

1
Cytoskeleton Normal Disrupted By immunofluorescent
staining under fluorescent
microscope

Surface antigens Cell surface antigens Extra antigens By RNA microarray by


examining the RNA
transcripts of transformed
cells and 2nd method is by
specific antibodies against
these antigens.

Anchorage dependence Present Absent Under inverted


microscope

Contact inhibition Present Absent. Thick layers of Under inverted


cells form in cell culture microscope
flask.

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