BIOL 2420

Staphylococcus epidermidis
Pseudomonas aeruginosa

Salmonella typhimurium

Streptococcus agalactiae

Streptococcus pyogenes
Enterobacter aerogenes

Klebsiella pneumoniae

Staphylococcus aureus
Enterococcus faecalis
Microbiology for the

Enterobacter cloacae
Bacillus megaterium
Alcaligenes faecalis

Micrococcus roseus

Serratia marcescens
Micrococcus luteus

Proteus mirabilis

Proteus vulgaris
Bacillus subtilis

Escherichia coli
Health Sciences
Biochemical Test Chart
(You will not fill in every blank in this chart.)
Colonial Morphology surface, consistency, soluble
(use standard abbreviations or pigment, optical, size, shape,
devise your own legend) margin, elevation, color, etc.

Cellular Morphology rods, cocci, pairs, chains,

tetrads, clumps, size, etc.

Motility + = motile
- = nonmotile
Gram Stain + = purple to blue stain
- = red to pink stain
Acid-Fast Stain + = acid fast (red)
- = non-acid fast (blue)
Capsule Stain + = capsules (halo around cells)
- = no capsules
Endospore Stain + = endospores (green)
- = no endospores (red)

Glucose Fermentation + = yellow (Check for gas)

- = red
Lactose Fermentation + = yellow (Check for gas)
- = red
Sucrose Fermentation + = yellow (Check for gas)
- = red
Casease + = clearing in agar
- = no clearing in agar
Gelatin Hydrolysis + = liquid
- = solid
Lipase (Tributyrin) + = clearing in agar
- = no clearing in agar
Starch Hydrolysis + = no color change in iodine
- = iodine turns blue/black
Indole + = red lake
- = no color change
Methyl Red + = red
- = yellow to orange
Voges Proskauer + = intense rose/red color
- = yellow
Citrate + = blue
- = green
 BIOL 2420

Staphylococcus epidermidis
Pseudomonas aeruginosa

Salmonella typhimurium

Streptococcus agalactiae

Streptococcus pyogenes
Enterobacter aerogenes

Klebsiella pneumoniae

Staphylococcus aureus
Enterococcus faecalis
Microbiology for the

Enterobacter cloacae
Bacillus megaterium
Alcaligenes faecalis

Micrococcus roseus

Serratia marcescens
Micrococcus luteus

Proteus mirabilis

Proteus vulgaris
Bacillus subtilis

Escherichia coli
Health Sciences
Biochemical Test Chart
(You will not fill in every blank in this chart.)
Hydrogen Sulfide + = black precipitate
- = no black precipitate
Nitrate + = red or brown on 1st step
- = red or brown on 2nd step
Urease + = bright pink
- = salmon color (no change)
Kligler Iron Agar see lab book:
Exercise 5-18, page 311
Lysine Decarboxylase see lab book:
Exercise 5-8, page 263
Ornithine see lab book:
Decarboxylase Exercise 5-8, page 263
DNase Agar + = clearing in agar
- = no clearing in agar
Phenylalanine + = green color
- = no color change
Deaminase
Blood Agar β = clear zone; α = green
zone; γ = no change
Mannitol Salt Agar + salt tolerance = growth
+ mannitol ferment. = yellow
Phenylethyl Alcohol + = good growth
- = poor to no growth
Agar
Catalase + = bubbles
- = no bubbles
Oxidase + = purple
- = no color change
Bile Esculin + = black
- = no color change
A disc + = zone of inhibition
- = no inhibition
P disc + = zone of inhibition
- = no inhibition
6.5% NaCl broth + = growth
- = no growth
Eosin Methylene + = dark w/ colorless edges
- = colorless; E. coli = green
Blue Agar
Hektoen Enteric Agar see lab book:
Exercise 4-5, page 217
MacConkey Agar + = red colonies, red agar
- = colorless colonies