Biology

Florida Biology 1
End-of-Course Assessment
Review and Practice
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SC.912.E.7.1 Biology
Analyze the movement of matter and energy through the different biogeochemical cycles,
including water and carbon.

STANDARD REVIEW
The Water Cycle
The movement of water between the oceans, atmosphere, land, and living things is known
as the water cycle. During evaporation, the Sun’s heat causes water to change from liquid to
vapor. In the process of condensation, the water vapor cools and returns to a liquid state.
The water that falls from the atmosphere to the land and oceans is precipitation. Some of
the precipitation that falls on land flows into streams, rivers, and lakes and is called runoff.
Some precipitation seeps into the ground and is stored in spaces between or within rocks.
This water, known as groundwater, will slowly flow back into the soil, streams, rivers, and
oceans.

WATER CYCLE

Condensation

Precipitation

Transpiration

Evaporation

Runoff

Lakes and oceans

Groundwater

The Carbon Cycle

Carbon is an essential substance in the fuels used for life processes. Carbon moves through
the environment in a process called the carbon cycle. Part of the carbon cycle is a short-
term cycle. In this short-term cycle, plants convert carbon dioxide from the atmosphere
into sugars and starches. Plants use these substances for energy, releasing carbon dioxide
into the air. Other organisms eat the plants to get the carbon. Like plants, the organisms
break down sugars for energy, releasing some of the carbon back into the air. The decay of
dead organisms and wastes also releases carbon into the air.

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Part of the carbon cycle is a long-term cycle in which carbon moves through the Earth
system over a very long period. Carbon is stored in the geosphere in buried plant or animal
remains. Fossil fuels, which contain carbon, formed from plant and animal remains that
were buried millions of years ago. Carbon is also stored in a type of rock called a
carbonate. Carbonate forms from shells and bones of once-living organisms.

CARBON CYCLE

Carbon dioxide in the air

Photosynthesis

Respiration
Combustion

Carbon in liv- ing things

Decomposition

Carbon in soil and rock

Carbon in fossil fuels
STANDARD PRACTICE
1 By what process do clouds form in the atmosphere?
A. condensation

B. decomposition

C. precipitation

D. respiration

2 Which of the following statements about groundwater is true?

A. It is salty like ocean water.

B. It never reenters the water cycle.

C. It stays underground for a few days.

D. It is stored in underground caverns or porous rock.

3 By what process is the carbon in fossil fuels released into the atmosphere?
A. combustion

B. decomposition

C. erosion

D. respiration

4 Which of the following sources of carbon takes the shortest time to form?
A. coal

B. limestone

C. natural gas

D. plant remains
Name Date

SC.912.L.14.1 Biology
Describe the scientific theory of cells (cell theory) and relate the history of its discovery to
the process of science.

SC.912.N.3.1

STANDARD REVIEW
When the English scientist Robert Hooke used a crude microscope to observe a thin slice
of cork in 1665, he saw “a lot of little boxes.” The boxes reminded him of the small rooms
in which monks lived, so he called them cells. Hooke later observed cells in the stems and
roots of plants. Ten years later, the Dutch scientist Anton van Leeuwenhoek used a
microscope to view water from a pond, and he discovered many living creatures. He named
them “animalcules,” or tiny animals. Today we know that they were not animals but
single-celled organisms.
It took scientists more than 150 years to fully appreciate the discoveries of Hooke and
Leeuwenhoek. In 1838, the German botanist Mattias Schleiden concluded that cells make
up not only the stems and roots but every part of a plant. A year later, the German
zoologist Theodor Schwann claimed that animals are also made of cells. In 1858,
Rudolph Virchow, a German physician, determined that cells come only from other cells.
The observations of Schleiden, Schwann, and Virchow form the cell theory, which has
three parts:

1. All living things are made of one or more cells.

2. Cells are the basic units of structure and function in organisms.
3. All cells arise from existing cells.

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STANDARD PRACTICE
1 The combined observations of Mattias Schleiden, Theodor Schwann, and Rudolph
Virchow resulted in the formation of the cell theory. Which of the following is part of
the cell theory?
A. All cells contain the same organelles.

B. All cells arise from carbon molecules.

C. All living things are made of one or more cells.

D. Cells are complex units of structure and function in organisms.

2 According to the cell theory, cells are the basic units of structure and function in
organisms. Which scientist observed plant cells and gave them the name “cells”?
A. Hooke

B. Leeuwenhoek

C. Schleiden

D. Schwann

3 A cheetah, like those shown below at left, and a paramecium, like the one shown
below at right, are both living things.

According to the cell theory, what can you conclude about these two very different
organisms?
A. They are made of many cells.

B. They are made of one or more cells.

C. They come from the same kind of cell.

D. They come from noncellular structures.

4 In science, a theory is an explanation for some phenomenon that is based on
observation, experimentation, and reasoning. One important theory in biology is the
cell theory. What kinds of observations led to the development of the cell theory?
A. testing of first vaccines

B. discoveries of fossil bacteria

C. isolations of DNA and RNA

D. microscopic views of plants and animals

Name Date

SC.912.L.14.2 Biology
Relate structure to function for the components of plant and animal cells. Explain the role
of cell membranes as a highly selective barrier (passive and active transport).

STANDARD REVIEW
Cells share common structural features, including an outer boundary called the cell
membrane. The cell membrane encloses the cell and separates the cell interior, called the
cytoplasm, from its surroundings. The cell membrane also regulates what enters and leaves
a cell—including gases, nutrients, and wastes.
Movement across the cell membrane that does not require energy from the cell is
called passive transport. One kind of passive transport, diffusion, is the movement of a
sub- stance from an area of high concentration to an area of lower concentration
caused by the random motion of particles of the substance. The diffusion of water
through a selectively permeable membrane is called osmosis. In facilitated diffusion, a
carrier protein transports a substance across the cell membrane down the concentration
gradient of the substance.
Active transport is the movement of a substance against the concentration gradient of
the substance. Active transport requires cells to use energy. In animal cells, the
sodium- potassium pump uses energy supplied by ATP to transport sodium ions out
of the cell and potassium ions into the cell. During endocytosis, substances are
moved into a cell by a vesicle that pinches off from the cell membrane. During
exocytosis, substances inside a vesicle are released from a cell as the vesicle fuses
with the cell membrane.
Within the cytoplasm are many structures, often suspended in a system of microscopic
fibers called the cytoskeleton. The cytoplasm includes organelles that carry out various life
processes. Organelles are structures that perform specific functions within the cell.
Different types of cells have different organelles. The table below summarizes the functions
of different organelles.

Table 1 Organelles and Their Functions

Nucleus Chloroplast
the organelle that contains the cell’s DNA and the organelle that uses the energy of sunlight
is the control center of the cell to make food

Ribosome Golgi complex

the organelle in which amino acids are the organelle that processes and transports pro-
hooked together to make proteins teins and other materials out of cell

Endoplasmic reticulum
the organelle that makes lipids, breaks down Large central vacuole
drugs and other substances, and packages the organelle that stores water and other
proteins for Golgi complex materi- als

Lysosome
Mitochondrion
the organelle that digests food particles, wastes,
the organelle that breaks down food molecules
cell parts, and foreign invaders
to make ATP

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STANDARD PRACTICE
1 The diffusion of water through a selectively permeable membrane is called osmosis.
Which description represents the effects of osmosis on a plant cell when the
concentration of sugar particles is greater inside the cell than outside?
A. Water diffuses into the cell, and the cell swells.

B. Water diffuses out of the cell, and the cell shrinks.

C. Water moves into and out of the cell at equal rates, and cell size remains the
same.
D. Water is blocked from moving into or out of the cell, and cell size remains the
same.

2 The illustration below shows a cell and a large particle. Many substances, such as
proteins and polysaccharides, are too large to be transported into a cell by carrier
proteins. In a process called endocytosis, the large particle is brought into the cell.

1 2 3

Which of the following statements correctly summarizes this process?

A. Waste materials can enter the cell by the same process.

B. The particle passes directly through a protein doorway in the cell membrane.

C. The particle passes directly through the phospholipids that make up the cell
membrane.
D. The cell membrane surrounds the particle and forms a vesicle that is brought
into the cell.
3 Cells can have two kinds of endoplasmic reticulum (ER): smooth ER, which has no
ribosomes, and rough ER, which has ribosomes embedded in its membrane. What
would happen if the rough endoplasmic reticulum in the cell of an organism lost all
of its ribosomes?
A. ATP production in the cell would stop.

B. Protein production in the organism would stop.

C. Storage of proteins within the cell would increase.

D. Proteins would no longer be exported from the cell.

4 An oxygen molecule comes into contact with the outside of a cell’s lipid bilayer. What
process would allow the molecule to move into the cell?
A. osmosis

B. active transport

C. simple diffusion

D. facilitated diffusion
Name Date

SC.912.L.14.3 Biology
Compare and contrast the general structures of plant and animal cells. Compare
and contrast the general structures of prokaryotic and eukaryotic cells.

STANDARD REVIEW
The smallest and simplest cells are prokaryotes, which are bacteria. A prokaryote is a
single-celled organism that lacks a nucleus and other internal compartments. Prokaryotic
cells depend on a strong cell wall to give the cell shape. A prokaryotic cell wall is made of
strands of polysaccharides connected by short chains of amino acids. Some prokaryotic
cell walls are surrounded by a structure called a capsule, which is also composed of
polysaccharides. The capsule enables prokaryotes to cling to almost anything, including
teeth, skin, and food. Many prokaryotes have flagella, which are long, threadlike structures
that protrude from the cell’s surface and enable movement.
The first cells with internal compartments were primitive eukaryotic cells, which
evolved about 2.5 billion years ago. A eukaryote, such as a plant or an animal, is an
organism whose cells have a nucleus. The nucleus is an internal compartment that
houses the cell’s DNA. Other internal compartments, or organelles, enable
eukaryotic cells to function in ways different from prokaryotes. An organelle is a
structure that carries out specific activities in the cell.
Many organelles—such as the endoplasmic reticulum, vesicles, Golgi apparatus,
lysosomes, and mitochondria—are found in both animal cells and plant cells. However,
plant cells have three additional structures that are not found in animal cells:

 The cell wall—composed of proteins and carbohydrates, including the polysac-

charide cellulose—surrounds the cell membrane of plant cells.
 Chloroplasts are organelles that use light energy to make carbohydrates
from carbon dioxide and water.
 The central vacuole is a large membrane-bound space that stores water and may
contain many substances, including ions, nutrients, and wastes; when full, it
makes the cell rigid.

Many single-celled eukaryotes use flagella for movement. Short hairlike structures called
cilia protrude from the surface of other eukaryotic cells. Flagella or cilia propel some cells
through their environment. In other cells, cilia and flagella move substances across the
cell’s surface.

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STANDARD PRACTICE
1 Plants play an important role in the carbon cycle by producing carbohydrates.
Which of the following organelles enables plants to make carbohydrates from
carbon dioxide and water?
A. cell wall

B. central vacuole

C. chloroplast

D. nucleus

2 The digestive system breaks down materials into substances the body can use. What
structure inside the cell is most similar to the digestive system in humans?
A. cytoplasm

B. lysosome

C. nucleolus

D. ribosome

3 The diagram below shows a cell.

STRUCTURE OF A CELL

What type of organism might contain this type of cell?

A. algae

B. animal

C. bacterium

D. plant
4 Cells may have different shapes and different amounts of organelles, depending on
their function. Which features do plant cells have that animal cells lack?
A. chloroplast, ribosome, and cell wall

B. Golgi apparatus, cytoskeleton, and vesicle

C. cell wall, chloroplast, and central vacuole

D. central vacuole, chloroplast, and smooth ER

Name Date

SC.912.L.14.4 Biology
Compare and contrast structure and function of various types of microscopes.

STANDARD REVIEW
Different types of microscopes have different qualities and uses. Light microscopes that use
two lenses are called compound light microscopes. In a typical compound light
microscope, a light bulb in the base shines light up through the specimen, which is
mounted on a glass slide. Both lenses magnify the image. Thus, a microscope with a
40× lens and a 10× lens produces a total magnification of 400×. The most powerful com-
pound light microscopes have a total magnification of up to 2,000×, which is sufficient for
viewing objects as small as 0.5 μm in diameter.
Electron beams have a much shorter wavelength than that of visible light, so electron
microscopes are much more powerful than light microscopes. Electron microscopes can
magnify an image up to 200,000×, and they can be used to study very small structures
inside cells or on cell surfaces. In electron microscopes, both the electron beam and the
specimen must be placed in a vacuum chamber so that the electrons in the beam will not
bounce off gas molecules in the air. Because living cells cannot survive in a vacuum, they
cannot be viewed using electron microscopes. Also, electron microscope images are
always in black and white unless scientists have added artificial colors to make certain
structures more visible.
Two kinds of electron microscopes are transmission electron microscopes and scanning
electron microscopes. In a transmission electron microscope, the electron beam is
directed at a very thin slice of a specimen stained with metal ions. A transmission electron
micrograph (TEM) can reveal a cell’s internal structure in fine detail. In a scanning
electron microscope, the electron beam is focused on a specimen coated with a very thin
layer of metal. A scanning electron micrograph (SEM) shows three-dimensional images of
cell surfaces.
The scanning tunneling microscope uses a needle-like probe to measure differences in
voltage caused by electrons that leak, or tunnel, from the surface of the object being
viewed. A computer tracks the movement of the probe across the object, enabling objects
as small as individual atoms to be viewed. The computer generates a three-dimensional
image of the specimen’s surface. The scanning tunneling microscope can be used to study
living organisms.

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STANDARD PRACTICE
1 Which of the following instruments produces highly magnified images of a cell’s
internal structure but cannot be used to examine living cells?
A. hand lens

B. light microscope

C. scanning electron microscope

D. transmission electron microscope

2 How does a microscope help scientists observe objects?

A. It measures objects.

B. It magnifies images.

C. It performs calculations.

D. It stains transparent objects.

3 A scientist examines a sample of tissue using the instrument shown below.

What kind of instrument is she using?

A. hand lens

B. light microscope
C. scanning electron microscope

D. transmission electron microscope

4 Why might a scientist use colored dyes when viewing items under a compound light
microscope?
A. to make them more visible

B. to make them more attractive

C. to make them appear more realistic

D. to make them appear more three-dimensional

5 Which of the following items is best observed under a compound light microscope?
A. an atom

B. a colony of ants

C. a single-celled organism

D. an underground fungal system

6 The Dutch scientist Anton van Leeuwenhoek used a microscope that made objects
appear 300 times as large as they were. If a cell appeared to be 6 mm long under the
microscope, how long was the cell in real life?
A. 0.02 mm

B. 0.05 mm

C. 0.20 mm

D. 0.50 mm
Name Date

SC.912.L.14.6 Biology
Explain the significance of genetic factors, environmental factors, and pathogenic agents to
health from the perspectives of both individual and public health.
HE.912.C.1.3

STANDARD REVIEW
In general, you can get infectious diseases in any of five different ways: through person-to-
person contact, air, food, water, and animal bites. They are caused by pathogens, which
are disease-causing agents. Pathogens can be bacteria, viruses, fungi, or protists. Diseases
transferred from person to person are considered contagious, or communicable. For exam-
ple, when a person sneezes, droplets of saliva and mucus carrying pathogens are expelled
from the mouth and nose. If another person breathes these droplets, the pathogens can
infect that person.
Some diseases are not infectious. They are instead caused by environmental factors, such
as poisons or a lack of a certain type of nutrient in the diet. Some are caused when a
person’s genetic material is damaged or copied incorrectly, resulting in genes that produce
faulty proteins. Changes in genetic material are called mutations. Mutations can
occur randomly. Or, they can be caused by exposure to environmental factors, such as
radiation or chemicals.
When a mutation occurs in the genetic material of an egg or sperm, it can be passed from
parent to offspring. Harmful effects produced by inherited mutations are called genetic
disorders. Some genetic disorders include sickle cell anemia, cystic fibrosis, hemophilia,
and Huntington’s disease.

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STANDARD PRACTICE
1 In the 1800s, many people living in cities in the United States died of infectious
diseases such as cholera, which is caused by a bacterium that pollutes water. Cholera
is no longer a major problem in the United States. What is the most likely reason for
the elimination of cholera as a major disease?
A. Advances in medicine have led to cures for cholera.

B. People have learned the importance of washing their hands.

C. Sewage treatment plants have eliminated such pathogens from drinking water.

D. Regulations have prevented factories from dumping pollution into lakes and
rivers.

2 Throughout history, amoebic dysentery has resulted in the deaths of many

soldiers. Deaths from this illness have often exceeded the number of deaths
from wounds.
What likely led to the high rate of infection by the amoeba that causes this disease?
A. lack of available vaccines
B. lack of doctors to treat the sick soldiers
C. lack of vitamins and minerals in the diet
D. unclean food and water on the battlefield

3 The diagram below shows an HIV particle.

What is the function of the glycoproteins on the outside of the virus?

A. to carry genetic material
B. to propel the virus forward
C. to help the virus invade cells
D. to protect the HIV particle from antibodies
4 Diseases can be caused by many different types of organisms, including protozoa.
Which of the following diseases is caused by a protozoan?
A. anthrax

B. influenza (flu)

C. malaria

D. tuberculosis

5 Although many types of bacteria are helpful and do not cause disease, nearly half of
all human diseases are bacterial. Better sanitation and the use of antibiotics over the
last century have affected death rates from bacterial infections in which way?
A. death rates have increased

B. death rates have decreased

C. death rates have remained the same

D. death rates have increased and then decreased

Name Date

SC.912.L.14.7 Biology
Relate the structure of each of the major plant organs and tissues to physiological
processes.

STANDARD REVIEW
Nearly all plants have a body that consists of a vertical shaft from which specialized
structures branch, as shown below. The part of a plant’s body that grows mostly upward
is called the shoot. In most plants, the part of the body that grows downward is called the
root. Zones of actively dividing plant cells, called meristems, produce plant growth. The
vertical body form results as new cells are made at the tips of the plant body.
Shoot meristems

Leaf

Stem

Root

Root meristems

Specialized cells that transport water and other materials within a plant are found in
vascular tissues. Relatively soft-walled cells transport organic nutrients in a kind of tissue
called phloem. Hard-walled cells transport water and mineral nutrients in a kind of tissue
called xylem, which also helps support the plant body.
A watertight covering called the cuticle, which reduces water loss, is a waxy layer that
covers the nonwoody aboveground parts of most plants. The cuticle does not let oxygen
or carbon dioxide pass through it. Pores called stomata (singular, stoma) permit plants to
exchange oxygen and carbon dioxide. A pair of specialized cells called guard cells border
each stoma, as seen below. Stomata open and close as the guard cells change shape.

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Some plants reproduce through seeds. A seed is a structure that contains the embryo of a
plant. An embryo is an early stage in the development of plants and animals. Most plants
living today are seed plants—vascular plants that produce seeds. Some, such as pine trees,
are gymnosperms, seed plants whose seeds do not develop within a sealed container
(a fruit). Most seed plants are flowering plants, or angiosperms. Angiosperms produce
seeds that develop enclosed within a specialized structure called a fruit.
STANDARD PRACTICE
1 The stomata on a saguaro cactus must open to let in carbon dioxide from the
atmosphere. When each stoma opens, however, water can escape from the plant. How
does the saguaro minimize the loss of water when stomata open?
A. Guard cells open and close the stomata rapidly during the day to let as little
water as possible escape.
B. Guard cells open the stomata only at night, when the air temperature is
cooler and the humidity somewhat higher.
C. Guard cells always keep the stomata open slightly, so that carbon dioxide can
flow in but very little water can flow out.
D. The saguaro has fewer stomata than any other plant, an adaptation that
prevents water loss through transpiration.

2 The diagram below shows a flower.

E
D
A

BC

Which parts of the flower are male reproductive structures?

A. parts A and B

B. parts C and D

C. parts E and F

D. parts D, E, and F
3 A unique characteristic of the banyan tree is that roots grow down from its branches
into the ground. The tree can appear to have several trunks. What advantage does this
root characteristic give the banyan tree over other trees?
A. The roots provide shelter for ground-dwelling animals, which carry nutrients to
the tree.
B. The banyan can grow near the equator, because aboveground roots are
more protected from the sun.
C. The banyan can only grow in humid climates, because aboveground roots
are more likely to dry out and die during droughts.
D. The banyan can grow in areas prone to hurricanes and typhoons, because the
roots make the tree more stable in high winds.

4 The drawing below shows fiddleheads from a fern.

A fiddlehead develops into what plant part?

A. stem

B. seed

C. flower

D. leaf or frond
Name Date

SC.912.L.14.26 Biology
Identify the major parts of the brain on diagrams or models.

STANDARD REVIEW
The brain, shown in the diagram below, is the body’s main processing center. It consists of
three major parts—the cerebrum, the cerebellum, and the brain stem.

Thalamus
Cerebrum
Hypothalamus
Corpus callosum

Cerebellum
Midbrain

Brain Pons Spinal cord

stem
Medulla
oblongata

The cerebrum is the largest part of the brain. The capacity for learning, memory,
perception, and intellectual function resides in the cerebrum. The cerebrum has a folded
outer layer with many bumps and grooves. A long, deep groove down the center divides
the cerebrum into right and left halves, or hemispheres. The cerebral hemispheres commu-
nicate through a connecting band of axons called the corpus callosum. Most sensory and
motor processing occurs in the cerebral cortex, the folded thin outer layer of the cerebrum.
The cerebellum regulates balance, posture, and movement. The cerebellum smooths and
coordinates ongoing movements, such as walking, by timing the contraction of skeletal
muscles. The cerebellum integrates and responds to information about body position from
the cerebrum and the spinal cord to control balance and posture.
The brain stem is a collection of structures leading down to the spinal cord and
con- necting the cerebral hemispheres with the cerebellum. The lower brain stem
consists of the midbrain, the pons, and the medulla oblongata. These structures
relay information
throughout the central nervous system and play an important role in homeostasis by
regu- lating vital functions, such as heart rate, breathing rate, body temperature, and
sleep.
The upper brain stem contains important relay centers that direct information to and
from different parts of the brain. The thalamus is a critical site for sensory processing.
Sensory information from all parts of the body converges on the thalamus, which relays
the informa- tion to appropriate areas of the cerebral cortex. The hypothalamus, along
with the medulla oblongata, helps regulate many vital homeostatic functions, such as
breathing and heart rate. The hypothalamus is responsible for feelings of hunger and
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Name Date

SC.912.L.14.26 Biology
thirst. It also regulates many functions of the endocrine system by controlling the
secretion of many hormones.

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STANDARD PRACTICE
1 Which part of the brain plays a major role in homeostasis by regulating such
processes as heart rate and breathing through the autonomic nervous system?
A. brainstem

B. cerebrum

C. hypothalamus

D. thalamus

2 The diagram shows that the hypothalamus stimulates a gland to release hormones.

Hypothalamus
(YPOTHALMUS
,(

&3(
!

!#4(

Which endocrine gland does the area marked A refer to?

A. testis

B. ovary

C. adrenal gland

D. anterior lobe of the pituitary

3 The diagram below shows parts of the central nervous system.

A
C

B
D

What is the function of part E?

A. learning and memorization

B. coordinating movement, balance, and posture

C. regulating functions of the endocrine system

D. allowing communication between both sides of the brain

4 Which structure connects the cerebral hemispheres with the cerebellum?

A. brain stem

B. corpus callosum

C. sensory receptor

D. spinal cord
Name Date

SC.912.L.14.36 Biology
Describe the factors affecting blood flow through the cardiovascular system.

STANDARD REVIEW
The human cardiovascular system is made up of blood vessels, blood, and the heart, which
together function to transport materials, remove wastes, and distribute heat. Blood vessels
allow for the movement of blood to all cells in the body. The pumping action of the heart,
however, is needed to provide enough pressure to move blood throughout the body. The
heart is made up mostly of cardiac muscle tissue, which contracts to pump blood.
The human heart has two separate circulatory loops. The right side of the heart is
responsible for driving the pulmonary circulation loop, which pumps oxygen-poor blood
through the pulmonary arteries to the lungs. Gas exchange—the release of carbon dioxide
and pick up of oxygen—occurs in the lungs. The oxygenated blood is then returned to the
left side of the heart through pulmonary veins.
The left side of the heart is responsible for driving the systemic circulation loop, which
pumps oxygen-rich blood through a network of arteries to the tissues of the body.
Oxygen-poor blood is then returned to the right side of the heart through the veins.
As blood flows through the blood vessels of the body, it provides cells with nutrients and
oxygen and carries away wastes and carbon dioxide. Blood consists of plasma (water,
metabolites, wastes, salts, and proteins), red blood cells, white blood cells, and platelets.
Red blood cells carry oxygen. White blood cells fight infection and disease. Platelets help
clot the blood and stop bleeding in a wound.

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STANDARD PRACTICE
1 A red blood cell has a flattened disc shape, as illustrated below.

What advantage does the shape of a red blood cell provide?

A. decreases its surface area-to-volume ratio in order to limit cell growth
B. increases its surface area-to-volume ratio so it can take in nutrients efficiently
C. increases its surface area-to-volume ratio so the cell can grow much larger
D. decreases its surface area-to-volume ratio so more cells can fit in a blood vessel

2 Some disorders result in a drastic drop-off in the number of platelets in the blood.
What effect would a low “platelet count” have on the body?
A. A person with this disorder would become anemic.

B. The risk of bleeding from a wound would increase.

C. The body would not be able to fight off infectious diseases.

D. The risk of stroke or heart attack would increase due to blood clots.

3 An investigation traces the concentration of oxygen gas contained in blood as the

blood passes through the circulatory system. Which choice below begins with the area
of the circulatory system where blood has the highest oxygen level and ends where
blood contains the lowest oxygen level?
A. capillaries, veins, right ventricle, lungs, left ventricle
B. lungs, left ventricle, capillaries, veins, right ventricle
C. right ventricle, lungs, left ventricle, capillaries, veins
D. veins, right ventricle, lungs, left ventricle, capillaries
4 Heart rate, or the rate at which the muscles in the heart contract, is controlled by the
sinoatrial node, a group of cells in the cardiac muscle of the right atrium. Heart rate
decreases when you are asleep and increases when you are awake. Some people
require an artificial pacemaker to regulate their heart rate if it is too slow. Which
hypothesis explains how the body might be affected by a dangerously slow heart rate?
A. The body might have trouble falling asleep or feeling rested after a full night of
sleep.
B. Cells in the body might not receive enough oxygen, which could cause the person
to faint.
C. The adrenal gland might not produce enough hormones to prepare the body
for a quick reaction.
D. Breathing might become shallow, which could cause the person to retain too
much carbon dioxide.

5 A lack of which component(s) might cause concern about blood-clotting ability in

someone about to undergo surgery?
A. platelets

B. red blood cells

C. white blood cells

D. red and white blood cells

Name Date

SC.912.L.14.52 Biology
Explain the basic functions of the human immune system, including specific and
nonspecific immune response, vaccines, and antibiotics.
HE.912.C.1.8

STANDARD REVIEW
The immune system consists of cells and tissues found throughout the body. The body uses
both nonspecific and specific defense mechanisms to detect and destroy pathogens, thereby
preventing or reducing the severity of infection.
The body’s surface defenses are nonspecific, meaning they do not target specific pathogens.
Your skin is the first of your immune system’s nonspecific defenses against pathogens. Skin
acts as a nearly impenetrable barrier to invading pathogens, keeping them outside the body.
Oil and sweat make the skin’s surface acidic, inhibiting the growth of many pathogens.
Sweat also contains the enzyme lysozyme, which digests bacterial cell walls. Mucous
membranes are layers of epithelial tissue that produce a sticky, viscous fluid called mucus
that serves as a barrier to pathogens and produces chemical defenses.
When pathogens break through your body’s first line of defense, four important
nonspecific defenses take action:

 Body temperature increases and slows the growth of bacteria.

 An inflammatory response causes local blood vessels to dilate, increasing blood
flow to the infection site.
 Blood flow brings white blood cells that can attack and kill pathogens.
 Blood flow also brings special proteins that kill or inhibit pathogens.

Pathogens that have survived the first and second lines of nonspecific defenses still face
a third line of specific defenses—the immune response. The immune response consists
of an army of individual cells that rush throughout the body to combat specific invad-
ing pathogens. Four main kinds of white blood cells participate in the immune response:
macrophages, cytotoxic T cells, B cells, and helper T cells. Each kind of cell has a different
function. Macrophages consume pathogens and infected cells. Cytotoxic T cells attack and
kill infected cells. B cells label invaders for later destruction by macrophages. Helper T cells
activate both cytotoxic T cells and B cells. Macrophages can attack any pathogen. B cells
and T cells, however, respond only to pathogens for which they have a genetically pro-
grammed match. These four kinds of white blood cells interact to remove pathogens from
the body.

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STANDARD PRACTICE
1 The hypothesis that germs cause disease is called germ theory. Before the 1880s, many
scientists believed in the idea of spontaneous generation—the sudden appearance of
living organisms from nonliving matter. Today germ theory is accepted as one of the
great discoveries of medicine. Which of the following is a way in which germ theory
has had an impact on modern health care?
A. the development of safer anesthetics

B. the development of genetic medicine

C. the development of vaccines against childhood diseases

D. the rise in public awareness about the causes of heart disease

2 The human immune system can produce a specific response or a nonspecific response
to infection. Which of the following is a specific immune response of the human
immune system?
A. fever

B. inflammation

C. antigen display V

D. interferon release

3 When a pathogen infects a host cell, the body produces specialized white blood cells
that detect and destroy the specific pathogen. Pathogens have unique proteins on their
surfaces called antigens. The specificity of the immune system is due to the antigen
receptors on immune cells. These antigen receptors bind to antigens that match their
shape exactly. Helper T cells are regulatory white blood cells with specific antigen
receptors on their surfaces. Activated helper T cells do not directly attack infected
body cells or pathogens. Instead, they grow and divide, producing more helper T cells
with identical receptors on their surfaces. The helper T cells activate the destruction of
the infected cells and the removal of extracellular pathogens from the body. How does
the presence of more helper T cells affect the immune response to a specific pathogen?
A. the more helper T cells, the more antibodies are produced

B. the more helper T cells, the less the body is able to react to a specific pathogen
C. the more helper T cells that are activated, the more robust the immune response V

D. the more helper T cells, the more infected body cells will be attacked and
destroyed
4 Louis Pasteur, a French microbiologist, developed a vaccine against rabies. In 1885,
he tested it on a young man infected with the microbe that causes rabies. Medical
researchers searching for a vaccine against the virus that causes AIDS say that a
vaccine against this disease faces years of testing. Why can’t doctors try out untested
vaccines on people today as Pasteur did in the 1880s?
A. Drug companies delay releasing vaccines because they need to maximize profits. A

B. There is much more unnecessary government red tape today than there was in
the 1880s.
C. Vaccines developed today are not as effective as those that were developed in the
1800s.
D. Ethical issues and laws prevent doctors from giving people vaccines that may
contain unknown hazards. V

5 In the immune system’s specific response, white blood cells can target specific types of
disease-causing microbes. How do white blood cells recognize invading microbes?
A. Receptor proteins on their surfaces bind to specific antigens. A

B. Helper T cells release antibodies that bind with the antigens.

C. Natural killer cells puncture and destroy the infected body cells.

D. Plasma cells bind to the viral antigens and mark them for destruction.

6 Two types of white blood cells are macrophages and natural killer cells. How are the
roles of a macrophage and a natural killer cell different?
A. Macrophages target specific cells, while natural killer cells kill all pathogens.

B. Macrophages ingest pathogens and dead cells, while natural killer cells only
target cancer cells.
C. Macrophages attack lymph cells, and natural killer cells clean up debris from
dead cells.
D. Macrophages ingest and kill pathogens, while natural killer cells puncture the cell
membranes of infected cells.
Name Date

SC.912.L.15.1 Biology
Explain how the scientific theory of evolution is supported by the fossil record,
comparative anatomy, comparative embryology, biogeography, molecular biology, and
observed evolutionary change.
SC.912.N.1.6

STANDARD REVIEW
In 1859, the English naturalist Charles Darwin published convincing evidence that species
evolve, and he proposed a reasonable mechanism explaining how evolution occurs. Darwin
proposed that individuals that have physical or behavioral traits that better suit their
environment are more likely to survive and will reproduce more successfully than those
that do not have such traits. Darwin called this differential rate of reproduction natural
selection. In time, the number of individuals that carry favorable characteristics that are
also inherited will increase in a population. And thus the nature of the population will
change—a process called evolution.
Darwin further suggested that organisms differ from place to place because their
habitats present different challenges to, and opportunities for, survival and
reproduction. Each species has evolved and has accumulated adaptations in response
to its particular environment. An adaptation is an inherited trait that has become
common in a population because the trait provides a selective advantage.
Scientists have found many different kinds of evidence that supports the theory of
evolution. Fossils offer the most direct evidence that evolution takes place. Evidence of
orderly change can be seen when fossils are arranged according to their age. The anatomy
and development of living things also shows evidence of evolution. For example, the
similarities of structures in different vertebrates provide evidence that all vertebrates share
a common ancestor. Biological molecules also show evolutionary relationships.
Differences in amino acid sequences and DNA sequences are greater between species that
are more distantly related than between species that are more closely related.

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STANDARD PRACTICE
1 When Darwin first proposed his theory of evolution by natural selection, the field of
genetics did not yet exist. In what way does genetic science now contribute to the
study of evolution?
A. Scientists can create organisms that were extinct using DNA from fossils and
better understand how they evolved.
B. Scientists can use genetic engineering to carry out the process of evolution over
just months instead of millions of years.
C. Scientists can compare the DNA from fossils in rock to determine evolutionary
relationships among extinct species.
D. Scientists can determine evolutionary relationships among living species by
comparing amino acid sequences coded for by DNA.

2 Scientists look at evidence to determine possible evolutionary relationships and

mechanisms. Which of the following provides strong evidence for evolution?
A. the fossil record -

B. forensic biology

C. phylogenetic trees

D. works of philosophy

3 The pictures below show similarities among the forelimbs of three mammals.

#AT'S LEG
C"3'2 ,&( $OLPHIN'S mIPPER
D/,0)*.'2 ',*00&1 "AT'S
B"3'2 5*.(WING

These similarities provide evidence for which of the following hypotheses?

A. Legs and wings may have evolved from flippers.

B. All mammals have evolved from an ancestor that was a bat.

C. A cat’s leg, a dolphin’s flipper, and a bat’s wing have identical functions.

D. Cats, dolphins, and bats may have had the same ancestor millions of years ago.
4 How does drug resistance develop in bacteria?
A. Unsanitary conditions allow all kinds of bacteria to breed, including those
that are antibiotic resistant.
B. In the bloodstream, different species of bacteria exchange genes and
become resistant to antibiotics.
C. Mutations in some bacterial genes make the bacteria stronger and better able to
defeat the body’s immune system.
D. In the presence of an antibiotic, bacteria with genes that make them resistant
survive and eventually take over the population.

5 Biologists look at how organisms are related and when they first appeared on Earth.
Which of the following is true about the organisms that live on Earth today?
A. All organisms that have ever lived on Earth can still be found alive today.

B. Some of the organisms alive today have been around for 4.6 billion years.

C. The organisms alive today are the same as the ones that are found in fossils.

D. The organisms alive today evolved from organisms that previously lived on
Earth.
Name Date

SC.912.L.15.4 Biology
Describe how and why organisms are hierarchically classified and based on evolutionary
relationships.
LA.910.4.2.2

STANDARD REVIEW
Modern classification of living things is based on a system developed by the Swedish
biologist Carl Linnaeus. It is organized into a ranked system of groups that increase in
inclusiveness. Similar genera are grouped into a family. Similar families are combined into
an order. Orders with common properties are united in a class. Classes with similar
characteristics are assigned to a phylum. Similar phyla are collected into a kingdom.
Similar kingdoms are grouped into domains. All living things are grouped into one of
three domains. Two domains, Archaea and Bacteria, are each composed of a single
kingdom of prokaryotes. The third domain, Eukarya, contains all four kingdoms of
eukaryotes.
Linnaeus’s classification system was based on his observation that organisms have
different degrees of similarity. For instance, a tiger resembles a gorilla more closely than
either resembles a fish. According to Darwin’s views, organisms that are more similar to
one another than they are to other organisms have descended from a more recent common
ancestor. Therefore, classification based on similarities should reflect an organism’s
phylogeny, that is, its evolutionary history. Inferring evolutionary connections from
similarities, however, can be misleading. Not all features—or characters—are inherited
from a common ancestor. Consider the wings of a bird and the wings of an insect. Both
enable flight, but the structures of the two kinds of wings differ.
Most biologists today analyze evolutionary relationships using cladistics. Cladistics is
a method of analysis that reconstructs phylogenies by inferring relationships based on
shared characters. Cladistics can be used to hypothesize the sequence in which different
groups of organisms evolved. To do this, cladistics focuses on the nature of the characters
in different groups of organisms.

© Houghton Mifflin Harcourt Publishing

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STANDARD PRACTICE
1 In the Linnaean system of classification, organisms are grouped in successive levels of
hierarchy based on similarities in their form and structure. The diagram below models
the eight basic levels of the modern Linnaean system.

Levels
1
2
3
4
5
6
7
8

Which level of the Linnaean system does level 8 represent in the figure?
A. class

B. domain

C. family

D. species

2 Which series represents the correct order of levels of classification, from broadest to
narrowest?
A. domain, kingdom, phylum, order, class, family, genus, species

B. domain, kingdom, phylum, class, order, family, genus, species

C. kingdom, phylum, domain, order, class, family, genus, species

D. species, genus, family, class, order, phylum, kingdom, domain

3 The diagram below shows the evolutionary relationships among five animals.

ANIMAL CLADOGRAM

What major characteristic is the same for all five animals?

A. All are carnivores.

B. All have backbones.

C. All spend their entire lives on land.

D. All maintain a constant body temperature.

 4 Below is part of a field guide that several students have been using to identify trees in
a local park.

a. Leaves are thin and needlelike (coniferous)Go to 2

b. Leaves are broad and fanlike (deciduous)Go to 6

a. Needles are over 2.5 cm long and are clusteredGo to 3

b. Needles are 1.25 cm long or lessGo to 4

(Pinus rigida)
a. Needles occur in clusters of 3Pitch pine (Pinus rigida)
b. Needles occur in clusters of 5Eastern white pine
(Pinus strobus)

The students notice that one plant has thin 1.2-cm needles that occur in clusters.
Which of the following inferences can be made?
A. The plant is deciduous.

B. The plant is a Pinus rigida.

C. The plant is an Eastern white pine.

D. The plant cannot be identified from the information provided.

Name Date

SC.912.L.15.5 Biology
Explain the reasons for changes in how organisms are classified.

STANDARD REVIEW
More than 2,000 years ago, the Greek philosopher and naturalist Aristotle grouped plants
and animals according to their structural similarities. Later Greeks and Romans grouped
plants and animals into basic categories such as oaks, dogs, and horses. Eventually each
unit of classification came to be called a genus (plural, genera), the Latin word for “group.”
Starting in the Middle Ages, genera were named in Latin. The science of naming and clas-
sifying organisms is called taxonomy.
Until the mid-1700s, biologists named a particular type of organism by adding descriptive
phrases to the name of the genus. These phrases sometimes consisted of 12 or more Latin
words. They were called polynomials (from poly, meaning “many,” and nomen, meaning
“name”). For example, the European honeybee once had a 12-part scientific name: Apis
pubescens, thorace subgriseo, abdomine fusco, pedibus posticis glabis, untrinque margine
ciliatus. As you can see, the polynomial could became very large and awkward. Poly-
nomials were often changed by biologists, so organisms were rarely known to everyone by
the same name.
A simpler system for naming organisms was developed by the Swedish biologist
Carl Linnaeus. Linnaeus used a two-word Latin name for each species. Linnaeus’s
two-word system for naming organisms is called binomial nomenclature (from bi,
meaning “two”). His two-part name for the European honeybee was Apis mellifera, the
genus name followed by a single descriptive word. This unique two-part name for a species
is now referred to as its scientific name.
Linnaeus worked out a broad system of classification for plants and animals in which an
organism’s form and structure are the basis for arranging specimens in a collection. The
genera and species that he described were later organized into a ranked system of groups
that increase in inclusiveness. The different groups into which organisms are classified
have expanded since Linnaeus’s time and now consist of eight levels: domain, kingdom,
phylum, class, order, family, genus, and species.

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STANDARD PRACTICE
1 Biologists used to name an organism by adding descriptive phrases to the name of the
genus. Why did Linnaeus develop a scientific name for a species composed of two
Latin words?
A. to help identify species and prevent Latin from becoming a dead language

B. to help scientists keep knowledge about species within the scientific community

C. to prevent confusion by preventing other, competing systems of classification

from developing
D. to provide a consistent naming system that would allow scientists all over the
world to easily identify an individual species

2 Linnaeus’s system was based on his judgment of the importance of various

similarities among living things. Scientists have traditionally used similarities in
appearance and structure to group organisms. However, this approach has proven
problematic. What is one way that this approach could be problematic?
A. Some groups look different but turn out not to be related.

B. Some groups look similar but turn out to be closely related.

C. There is always a clear pattern between appearance and relatedness.

D. There is no consistent relationship between structures and relatedness.

3 Protists are classified using a different system than that used for most other types of
organisms. How does the system used to classify protists differ from other
classification systems?
A. Unlike other systems, the system used to classify protists has not changed
significantly in decades.
B. Because protists are similar to each other, the classification of protists is much
simpler than other classifications.
C. Because protists share characteristics with members of other kingdoms,
molecular sequencing is critical to classifying protists.
D. Because there are few fossils of protists, the classification of protists is based on
shared characteristics of only living species.
4 The ancient Greeks grouped plants and animals according to their structural
similarities. What are modern classification systems based on?
A. solely on structural characteristics of organisms

B. on similar behaviors as well as similar characteristics

C. solely on evolutionary relationships between organisms

D. on evolutionary relationships as well as similar characteristics V

5 In addition to the six kingdoms that all organisms are divided into, many scientists
also recognize three domains. These domains are divided by five characteristics: cell
type, the presence of cell walls, body type, nutrition, and genetics. What is the main
division of the way nutrition is gathered?
A. prokaryotic versus eukaryotic

B. unicellular versus multicellular

C. extremophiles versus methanogens V

D. autotrophs versus heterotrophs

Name Date

SC.912.L.15.6 Biology
Discuss distinguishing characteristics of the domains and kingdoms of living organisms.

STANDARD REVIEW
For many decades, scientists recognized two basic forms of life, prokaryotes and
eukaryotes. Then, scientists showed that the group of prokaryotes that make up the
kingdom Archaebacteria are more closely related to eukaryotes than they are to the other
kingdom of prokaryotes, Eubacteria. Thus, now living things are classified into three
domains.
The domain thought to be the oldest is Bacteria, which is composed of the organisms in
the kingdom Eubacteria. Archaea is the second prokaryotic domain and is also composed
of a single kingdom, Archaebacteria. A third domain, Eukarya, contains all four of the
eukaryotic kingdoms: Animalia (animals), Fungi (fungi), Plantae (plants), and Protista
(protists). The table below summarizes the major characteristics of the organisms in the six
kingdoms and three domains.

Domain and Kingdom Characteristics

Domain Kingdom Characteristics
Cell type Cell structure Body type Nutrition Example
Bacteria Eubacteria Prokaryotic Cell wall, Unicellular Autotrophic Enterobac-
peptidoglycan and teria
heterotrophic Spirochetes
Archaea Archae- Prokaryotic Cell wall, no Unicellular Autotrophic Methanogens
bacteria peptidoglycan and
heterotrophic
Eukarya Protista Eukaryotic Mixed Unicellular Autotrophic Amoebas
and multi- and Euglenas
cellular heterotrophic Kelps
Eukarya Fungi Eukaryotic Cell wall, Unicellular Heterotrophic Yeasts
chitin and multi- Mushrooms
cellular
Eukarya Plantae Eukaryotic Cell wall, Multicel- Autotrophic Ferns
cellulose lular Pine trees
Eukarya Animalia Eukaryotic No cell wall Multicel- Heterotrophic Birds
lular Earthworms

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STANDARD PRACTICE
1 Scientists find a new organism that is composed of many cells, gets its nutrition
from decaying organisms, and has cell walls. To what kingdom would the new
organism belong?
A. Animalia B. Eubacteria

C. Fungi V D. Protista

2 Which of the following properties could be used to distinguish between an organism

in the domain Bacteria and one in the domain Eukarya?
A. contains membrane-bound organelles V

B. uses energy to carry out multiple functions

C. uses simple mechanical motion to move around

D. is composed of organic chemicals such as amino acids

3 The Venn diagram below compares the two kingdoms Archaebacteria and
Eubacteria.

ArchAebActeriA eubActeriA
cell wall does not
cell wall contains
contain peptidoglycan
prokaryotic peptidoglycan
first found in extreme
single-celled found almost everywhere
environments
gene translation different
gene structure similar
from eukaryotes or
to eukaryotes
archaebacteria

Which of the following scientific explanations supports the division of archaea, or

archaebacteria, and bacteria into two different domains?
A. Archaea cause disease, but bacteria do not.

B. Bacteria have a nucleus, but archaea do not.

C. Archaea are single-celled, but bacteria often have more than one cell.
D. Archaea and bacteria exhibit differences in cell walls, cell membranes, and gene
Vstructure.
 4 Some people confuse slime molds with fungi that are also called molds. What would
be a reason that taxonomists chose to classify slime molds as protists rather than
fungi?
A. Slime molds are able to reproduce using spores.

B. Slime molds live in cool, moist places in a forest.

C. Slime molds are able to decompose small bits of rotting matter.

D. Slime molds are able to move during certain phases of their life. V
V
Name Date

SC.912.L.15.8 Biology
Describe the scientific explanations of the origin of life on Earth.
SC.912.N.1.3

STANDARD REVIEW
Scientist who study the origins of life think that the path to the development of living
things began when molecules of nonliving matter reacted chemically during the first billion
years of Earth’s history. These chemical reactions produced many different simple, organic
molecules. Energized by the sun and volcanic heat, these simple, organic molecules
formed more-complex molecules that eventually became the building blocks of the first
cells.
In the 1920s, the Russian scientist A. I. Oparin and the British scientist J.B.S. Haldane
both suggested that the early Earth’s oceans contained large amounts of organic mol-
ecules. This hypothesis became known as the primordial soup model. Earth’s vast oceans
were thought to be filled with many different organic molecules. Oparin and Haldane
hypothesized that these molecules formed spontaneously in chemical reactions activated by
energy from solar radiation, volcanic eruptions, and lightning.
In 1953, the primordial soup model was tested by Stanley Miller and Harold Urey.
Miller placed the gases that he and Urey proposed had existed on early Earth into a
device made up of glass tubes and vessels. To simulate lightning, he provided
electrical sparks. After
a few days, Miller found a complex collection of organic molecules, including some of
life’s basic building blocks: amino acids, fatty acids, and other hydrocarbons. These results
support the hypothesis that some basic chemicals of life could have formed spontaneously
under conditions like those in the experiment.
Scientists have reevaluated the Miller-Urey experiment in light of the fact that we now
know that four billion years ago, Earth did not have a protective layer of ozone gas, O 3.
Without ozone, ultraviolet radiation would have destroyed any ammonia and methane
present in the atmosphere.
In 1986, the geophysicist Louis Lerman suggested that the key processes that formed the
chemicals needed for life took place within bubbles beneath the ocean’s surface. In this
bubble model, he proposed that ammonia, methane, and other gases resulting from the
numerous eruptions of undersea volcanoes were trapped in underwater bubbles. Inside the
bubbles, these gases might have been protected from damaging ultraviolet radiation and
could have undergone chemical reactions. Eventually, the bubbles rose to the surface and
burst, releasing simple organic molecules into the air. In the air, the simple organic
molecules were exposed to ultraviolet radiation and lightning, which provided energy for
further reactions. The more-complex organic molecules that formed fell into the ocean
with rain, starting another cycle.

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STANDARD PRACTICE
1 Two models of the origin of life on Earth are the primordial soup model and
the bubble model. What do these two models of how life began on Earth have
in common?
A. Both explain how UV radiation produces ammonia and methane.

B. Both involve only chemical reactions that take place within the ocean.

C. Both include chemical reactions that take place when there is lightning. V

D. Both involve only chemical reactions that take place within the atmosphere.

2 American scientists Stanley Miller and Harold Urey used an apparatus similar to the
one in the diagram below to simulate how life could have formed on Earth. The
apparatus contained hydrogen gas, water vapor, ammonia, and methane. These gases
were subjected to a spark, to simulate lightning.

Spark
CH4
H2
NH3
Condenser
H2 O
vapor

Hot
Collecting
chamber
water
Organic
compounds

They found that organic molecules could form from the inorganic gases. Their
theory of how life began, however, did not hold up under further testing. What
crucial fact did they not have when they conducted their experiment?
A. There was no lightning in Earth’s early atmosphere.

B. Ammonia and methane were not in the early atmosphere.

C. The chemicals used in the experiment were contaminated. V

D. Organic molecules already existed before the atmosphere formed.

3 The Miller-Urey experiment showed that, under certain conditions, organic
compounds could form from inorganic molecules. What is one consequence of this
experiment?
A. Scientists think that life could not have developed through natural chemical and
physical processes.
B. The experiment proved that methane and ammonia will always give rise to
organic molecules in any circumstance.
C. Scientists think it is possible that organic compounds formed from the inorganic
compounds present on Earth billions of years ago. V
D. The experiment used the exact inorganic compounds present on Earth billions of
years ago and left little doubt about the mechanism of early life.

4 The bubble model states that that the key processes that formed the chemicals needed
for life took place within bubbles beneath the ocean’s surface. What important role
did bubbles play according to the bubble model?
A. They provided protection from damaging ultraviolet radiation. V

B. They allowed the reactions to take place in the presence of oxygen.

C. They kept the chemical products from ever entering Earth’s atmosphere.

D. They gave the activation energy needed for spontaneous chemical reactions.
Name Date

SC.912.L.15.10 Biology
Identify basic trends in hominid evolution from early ancestors six million years ago to
modern humans, including brain size, jaw size, language, and manufacture of tools.

STANDARD REVIEW
A primate is a member of the mammalian order Primates. Hominids are primates that
walk upright on two legs. Hominids are members of the group that led to the evolution of
humans. According to the fossil record, hominids first appeared on Earth about 5 mil-
lion to 7 million years ago. The early hominids best represented by fossil finds belong to
the group known as australopithecines. Australopithecines belong to the genus Austra
lopithecus. Their brains were generally as large as those of modern chimpanzees. They were
much smaller, however, than the brains of modern humans.
Our genus, Homo, is composed of at least three species. The first members of the genus
Homo appeared on Earth more than 2 million years ago. In the early 1960s, stone tools
were discovered near hominid bones. Because of its association with tools, this hominid
was named Homo habilis. The Latin word homo means “man,” and the Latin word habilis
means “handy.” Fossils indicated that Homo habilis lived in Africa for about 500,000 years
and then became extinct.
The species that replaced Homo habilis is called Homo erectus. Homo erectus was larger
than Homo habilis and also had a large brain. This species evolved in Africa and migrated
into Asia and Europe. Homo erectus survived for more than 1 million years. The species
disappeared about 200,000 years ago, as early modern humans emerged. Most scientists
think that Homo erectus was the direct ancestor of our species, Homo sapiens.
Of the three modern humans, Homo sapiens is the only surviving species of the genus
Homo. The name Homo sapiens is from the Latin homo, meaning “man,” and sapiens,
meaning “wise.” Early Homo sapiens left behind many fossils and artifacts, including the
first known paintings.

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STANDARD PRACTICE
1 Which species name meaning “handyman” was given to a set of hominid fossils
found near stone tools?
A. Homo erectus

B. Homo habilis V

C. Homo neanderthalensis

D. Homo sapiens

2 How do hominids differ from other primates?

A. They are bipedal. V

B. They have long arms.

C. They have grasping hands.

D. They have binocular vision.

3 The following table classifies three different organisms.

CLASSIFICATION OF THREE DIFFERENT ORGANISMS

Organism Class A Family Genus

Bacterium Scotobacteria Spirochaetales Spirochaetaceae Cristispira
Box elder Dicotyledones Sapindales Aceraceae Acer
Human Mammalia Primates Hominidae B

Which level of classification is represented by the box labeled A?

A. division

B. kingdom

C. order V

D. phylum
4 What advantage did the development of bipedalism most likely confer to early
hominids?
A. It allowed them to see with binocular vision.

B. It allowed them to evolve an opposable thumb. V

C. It allowed them to evolve a complex social structure.

D. It allowed them to move and hold objects at the same time.

5 A primate is a member of the mammalian order Primates, which have grasping

hands and feet and forward orientation of the eyes. Which of the following is not a
primate?
A. hominid

B. monkey

C. shrew V

D. tarsier
Name Date

SC.912.L.15.13 Biology
Describe the conditions required for natural selection, including: overproduction
of offspring, inherited variation, and the struggle to survive, which result in
differential reproductive success.
SC.912.N.1.4

STANDARD REVIEW
Evolution is a change in the characteristics of a population from one generation to the
next. Darwin proposed that evolution happened due to natural selection. Natural selection
is the process by which individuals that have favorable variations and are better adapted
to their environment survive and reproduce more successfully than less well adapted
individuals do. Over many generations, natural selection can result in the evolution of new
species, which is called speciation. The diagram below shows how natural selection changes
populations.

1 Overproduction
Each species produces more individuals than can survive to maturity.

3 Struggle to Survive
2 Genetic VariationIndividuals must compete The individuals of a populationwith each other for limited
may differ in traits such as size,resources. Also, some individuals color, strength, speed, abilitywill be harmed by predation,
to find food, or resistance todisease, or unfavorable conditions. certain diseases.

4 Differential Reproduction
Individuals that have certain traits are more likely to survive and reproduce than are individuals that lack those traits. Over time, those traits become more frequent in the population.

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STANDARD PRACTICE
1 Darwin developed his idea of evolution by natural selection based in part on
observations he made in the Galápagos Islands. Which of the following ideas
influenced Darwin’s development of evolutionary theory?
A. the discovery that genes are made of a biochemical called DNA

B. the idea formulated by ancient Greek philosophers that all substances are made
of atoms
C. the development of the cell theory based on ideas from Mattias Schleiden,
Theodor Schwann, and Rudolph Virchow
D. the observation by British economist Thomas Malthus that the human
popula- tion could not continue growing faster than the food supply V

2 Evolution is a change in the characteristics of a population from one generation to

the next. Which of the following is the correct order for the steps of evolution by
natural selection?
A. overproduction—selection—adaptation—variation

B. overproduction—selection—adaptation—evolution

C. overproduction—variation—selection—adaptation V

D. selection—variation—adaptation—overproduction

3 A population of crabs living on a sandy beach exhibits three colors: dark brown,
light brown, and speckled, as shown below. The genotypes for these colors are BB
for dark brown, bb for light brown, and Bb for speckled. The speckled color blends
in extremely well with the color of the sand on the beach. The pattern appears to
provide the speckled crabs with some protection from predatory birds.

PHENOTYPES AND GENOTYPES OF CRABS

Speckled (Bb) Dark Brown (BB) Light Brown (bb)

 Which of the following conclusions can be drawn from the information above?
A. Only the speckled crabs will survive, and all of their offspring will be speckled.

B. The allele for light brown color will be lost because of predatory birds
eating light brown crabs.
C. The allele for dark brown color will be lost because of predatory birds
eating dark brown crabs.
D. Both the light brown and dark brown alleles will continue to be passed on in
the crab population. V

4 Speciation is the formation of new species as a result of evolution by

natural selection. What effect could separation of populations have on
speciation?
A. One half of the species will go extinct if the population is separated.

B. The separated populations will always evolve into at least two different species.

C. If the environments differ enough, the separated populations may evolve

differently. V
D. By separating, the populations will no longer be able to interbreed and will die
off.

5 The diagram below shows a sampling of Galápagos finches.

The evolution of beak sizes in Galápagos finches is a response to which of the

following?
A. the color of their beaks

B. the types of seeds available V

C. whether the populations interbreed

D. the nutritional content of the seeds they eat

6 Which of the following mutations would be most likely to improve the chances that
an organism would survive and reproduce?
A. a stronger scent that makes an animal easier to find

B. a weaker scent that makes a flower less attractive to bees

C. weaker eyesight that makes an animal less likely to find prey

D. stronger leg muscles that allow an animal to jump away from danger V
Name Date

SC.912.L.15.14 Biology
Discuss mechanisms of evolutionary change other than natural selection such as genetic
drift and gene flow.

STANDARD REVIEW
The Hardy-Weinberg principle states that the frequencies of alleles in a population do not
change unless evolutionary forces act on the population. However, genetic changes in a
population can be caused by mutation and natural selection. Three other forces that cause
evolutionary change are gene flow, nonrandom mating, and genetic drift.
The movement of individuals from one population to another can cause genetic change.
The movement of individuals to or from a population, called migration, creates gene flow,
the movement of alleles into or out of a population. Gene flow occurs because new
individuals (immigrants) add alleles to the population and departing individuals (emi-
grants) take alleles away.
Sometimes individuals prefer to mate with others that live nearby or are of their own
phenotype, a situation called nonrandom mating. Mating with relatives (inbreeding) is a
type of nonrandom mating that causes a lower frequency of heterozygotes than would
be predicted by the Hardy-Weinberg principle. Inbreeding does not change the frequen-
cies of alleles, but it does increase the proportion of homozygotes in a population. For
example, populations of self-fertilizing plants consist mostly of homozygous individuals.
Nonrandom mating also results when organisms choose their mates based on certain traits.
In animals, females often select males based on their size, color, ability to gather food, or
other characteristics.
In small populations, the frequency of an allele can be greatly changed by a chance event.
For example, a fire or landslide can reduce a large population to a few survivors. When an
allele is found in only a few individuals, the loss of even one individual from the
population can have major effects on the allele’s frequency. Because this sort of change
in allele frequency appears to occur randomly, as if the frequency was drifting, it is
called genetic drift. Small populations that are isolated from one another can differ
greatly as a result of genetic drift. The cheetah, for example, is a species whose
evolution has been seriously affected by genetic drift, and each cheetah is almost
genetically uniform with other members of the population.

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STANDARD PRACTICE
1 Gene flow is one force that can cause evolutionary change. Which example best
illustrates gene flow?
A. A flock of migrating geese enters into the territory of another flock and begins
interbreeding.
B. A flock of geese becomes isolated on an island, and over time, the population
begins to exhibit new characteristics.
C. Some individuals in a flock of geese have genes that are more advantageous, and
over time, the numbers of these geese increase.
D. A flock of migrating geese loses its way to its wintering grounds, leaving other
geese without competition for resources and enabling those geese to produce
more offspring.

2 Evidence exists that during the millions of years in which the Grand Canyon formed,
the canyon divided a single population of tufted-eared squirrels into two populations.
One of these squirrel populations, the Kaibab squirrel, now lives in isolation from the
other tufted-ear squirrels on the North Rim of the canyon. The other population, the
Abert’s squirrel, lives on the South Rim. Some biologists think that the two
populations of squirrels have evolved into two separate species. Other biologists think
that the Kaibab squirrel is a subspecies of the Abert’s squirrel.

Feature Abert’s squirrel Kaibab squirrel

Range western United States; Mexico North Rim; Kaibab Plateau
Habitat ponderosa pine forest ponderosa pine forest
Belly white black
Tail white white
Ears tufted tufted
Predators hawks; human hunters hawks
 The cladogram shows the evolution of the original tufted-ear squirrel population into
two separate species. Which of the following factors would make the evolution of the
squirrel population into separate species less likely?
A. Different predators live on the North and South Rims of the canyon.

B. A few of the squirrels manage to cross the canyon and breed with squirrels on
the other side.
C. Different trees grow on the North and South Rims of the canyon because of
changes in the water table.
D. A disease attacks one population of squirrels and kills most of them.
The squirrels on the other side of the canyon are not affected.

3 Charles Darwin published his theory of evolution in 1859. In what way does modern
evolutionary theory differ from the theory as proposed by Darwin?
A. Darwin inferred that individuals can evolve, but modern genetic science has
shown that this is not true.
B. Darwin inferred that individuals do not evolve, but modern genetic science has
shown that this is not true.
C. Modern science has disproved most of Darwin’s original theory of evolution,
because Darwin knew nothing about genes and their role in heredity.
D. Genetic studies have shown that gene expression and other factors operate along
with natural selection, but most of Darwin’s theory has been supported by
modern science.

4 Genetic drift states that the random effects of everyday life can cause differences in
the survival and reproduction of individuals. What is one effect of genetic drift on
evolution?
A. It produces only the traits in a population that are best adapted to
the environment.
B. It can cause an unusual amount of genetic variation within a single
population of a species.
C. It can cause a population’s allele frequencies to become those predicted by the
Hardy-Weinberg principle.
D. It can cause certain traits in a population to increase even if those traits are not
the ones that are best adapted to the environment.
Name Date

SC.912.L.15.15 Biology
Describe how mutation and genetic recombination increase genetic variation.
HE.912.C.1.4

STANDARD REVIEW
Scientists now know that genes are responsible for inherited traits. Therefore, certain
forms of a trait become more common in a population because more individuals in the
popula- tion carry the alleles for those forms. In other words, natural selection causes
the frequency of certain alleles in a population to increase or decrease over time.
Mutations and the recombination of alleles that occurs during sexual reproduction
provide endless sources of new variations for natural selection to act upon.
Although mutation from one allele to another can eventually change allele frequencies,
mutation rates in nature are very slow. Most genes mutate only about 1 to 10 times per
100,000 cell divisions, so mutation does not significantly change allele frequencies,
except over very long periods of time. Furthermore, not all mutations result in phenotypic
changes. Recall that more than one codon—3-base DNA coding sequence—can code for
the same amino acid. Therefore, some mutations may result in no change in the amino acid
coded for in a protein, and other changes in an amino acid that do occur may not affect
how the protein works. Mutation is, however, an important source of variation and thus
makes evolution possible.
Meiosis and the joining of gametes—processes that recombine alleles—are essential to
evolution. No genetic process generates variation more quickly. In many cases, the pace of
evolution appears to increase as the level of genetic variation increases. For example, when
domesticated animals such as cattle and sheep are bred for large size, many large animals
are produced at first. But as the existing genetic combinations become used up, the ability
to obtain larger and larger animals slows down. Further progress must then wait for the
formation of new gene combinations.
The pace of evolution is sped up by genetic recombination. The combination of genes
from two organisms results in a third type, not identical to either parent. But bear in
mind that natural selection does not always favor genetic change. Indeed, many
modern organ- isms are little changed from their ancestors of the distant past. Natural
selection may favor existing combinations of genes, slowing the pace of evolution.

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STANDARD PRACTICE
1 One way that populations can change is when new alleles appear. What is the major
source of new alleles in natural populations?
A. mutations in sex cells

B. mutations in somatic cells

C. trait selection by natural selection

D. adaptations in individual organisms

2 At first, a mutation may make no difference to an individual. Even if the mutation

results in a nonfunctional protein, the body’s cell may have a functional copy of the
gene as its second allele. However, this new nonfunctioning version could be passed
on as a recessive allele. This kind of mutation is the probable origin of many recessive
disorders. Only characteristics that are expressed can be targets of natural selection.
Therefore, natural selection cannot operate against recessive alleles, even if they are
unfavorable. What does this explain?
A. why recessive alleles are never expressed

B. why genetic disorders can persist in a population

C. why advantageous offspring are more likely to survive and reproduce

D. why natural selection can act only against heterozygous carriers of a recessive
disorder

3 Imagine that a mouse has white fur because of a mutation in its DNA. Which of the
following conclusions can be drawn?
A. The white mouse increases the diversity of the species.

B. The white mouse decreases the diversity of the species.

C. The internal organs of the white mouse must not function as well as those of
other mice.
D. The white mouse is more likely to survive than other mice because it is
more visible to predators.
4 During meiosis, homologous chromosomes line up next to each other. In some cases,
one arm of a chromatid crosses over the arm of another chromatid. What is the result
of this process?
A. the creation of an additional sex cell

B. continuity in the offspring cell’s DNA

C. the independent assortment of genetic material

D. additional variation in the DNA of each sex cell formed

Name Date

SC.912.L.16.1 Biology
Use Mendel’s laws of segregation and independent assortment to analyze patterns of
inheritance.
SC.912.N.3.4

STANDARD REVIEW
Before the experiments of Gregor Johann Mendel in the mid-1800s, many people thought
offspring were a blend of the traits of their parents. For example, if a tall plant were
crossed with a short plant, the offspring would be medium in height. Mendel’s results did
not support the blending hypothesis. Instead, he developed these four hypotheses based
directly on the results of his experiments:

1. For each inherited character, an individual has two copies of the gene—one from
each parent.
2. There are alternative versions of genes. For example, the gene for flower color
in peas can exist in a “purple” version or a “white” version. An individual
receives one version, or allele, from each parent. Each allele can be passed on
when the individual reproduces.
3. When two different alleles occur together, one of them may be completely
expressed, while the other may have no observable effect on the organism’s
appearance. Mendel described the expressed form of the character as dominant.
The trait that was not expressed when the dominant form was present was
described as recessive. For example, if a plant has both purple and white alleles
for flower color but blooms purple flowers, then purple is the dominant form;
white is the recessive form.
4. When gametes are formed, the alleles for each gene in an individual separate
independently of one another. Thus, gametes carry only one allele for each inherited
character. When gametes unite during fertilization, each gamete contributes one
allele. Each parent can contribute only one of the alleles because of the way
gametes are produced during the process of meiosis.

Mendel’s hypotheses brilliantly predicted the results of his crosses. Similar patterns of
heredity have since been observed in countless other organisms. Because of their impor-
tance, Mendel’s ideas are often referred to as the laws of heredity:
The Law of Segregation: The two alleles for a single gene segregate (separate)
when gametes are formed.
The Law of Independent Assortment: The alleles of different genes separate
independently of one another during gamete formation.

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STANDARD PRACTICE
1 What evidence in pea plant crosses supported Mendel’s law of independent
assortment?
A. When crossing true-breeding pea plants, all of the offspring exhibit traits that are
exhibited in the parents.
B. When crossing hybrid pea plants, some of the offspring exhibit traits that are not
exhibited in the parents.
C. When crossing pea plants that differ in two characteristics, traits produced by
dominant factors always appear together.
D. When crossing pea plants that differ in two characteristics, traits produced by
dominant factors do not necessarily appear together.

2 For a certain plant, purple flowers (allele: P) are dominant, and white flowers (allele:
p) are recessive. A purple plant carrying both types of alleles is crossed with a true-
breeding white plant. What are the possible genotypes (allele pairs) of the offspring?
A. pp only B. Pp only

C. Pp and pp only D. PP, pp, and Pp

3 Rasheed crossed plants that were hybrid—have two different alleles—for the traits of
flower color, seed color, and seed shape as part of an investigation designed to verify
the results of Gregor Mendel’s experiments. The table below displays the results.

RASHEED’S RESULTS
Characteristic Offspring phenotypes Ratio
flower color 705 purple 224 white 3:1
seed color 6,002 yellow 2,001 green 3:1
seed shape 5,474 round 1,850 wrinkled 3:1

Based on the information in the table above, which of the following is a

valid conclusion?
A. White flowers are dominant.

B. Yellow seeds are a recessive trait.

C. Wrinkled seeds are a recessive trait.

D. Flower color is the result of environmental conditions.

4 A lily has one allele for spotted pink petals and one allele for solid pink petals.
The resulting flower has spotted pink petals. What must be true?
A. The allele for solid pink petals is recessive.

B. The allele for spotted pink petals is dominant.

C. The alleles for spotted and solid pink petals are both recessive.

D. The alleles for spotted and solid pink petals are both dominant.
Name Date

SC.912.L.16.2 Biology
Discuss observed inheritance patterns caused by various modes of inheritance, including
dominant, recessive, codominant, sex-linked, polygenic, and multiple alleles.

STANDARD REVIEW
Modern genetics is based on Gregor Johann Mendel’s explanations for the patterns of
heredity that he studied in garden pea plants. Mendel’s first experiments used monohybrid
crosses and were carried out in three steps, which are shown below.

1 Producing a true-
breeding P generation 2 Producing an 3 Producing
F1 generation F2 generation

Self-pollination
P generation

Cross-pollination

Self-pollination Self-pollination
F1 generation F2 generation
P generation All 705 purple : 224 white
purple

For each of the seven characteristics that Mendel studied in this experiment, he found a
similar 3-to-1 ratio of contrasting traits in the F 2 generation. Mendel’s experiments showed
that offspring do not show a trait for every allele they receive. Instead, combinations of
alleles determine traits. The set of alleles that an individual has for a characteristic is called
the genotype. The trait that results from a set of alleles is the phenotype. In other words,
genotype determines phenotype. Phenotype can also be affected by conditions in the
environment, such as nutrients and temperature. If an individual has two of the same
alleles of a certain gene, the individual is homozygous for the related character. On the
other hand, if an individual has two different alleles of a certain gene, the individual is
heterozygous for the related character. In the heterozygous case, the dominant allele is
expressed.
Although Mendel was correct about the inheritance of the traits he studied, most patterns
of inheritance are more complex than those that Mendel identified. First, not all genes
have only two alleles. There can be multiple alleles. Second, not all characteristics are
controlled by one gene. Other patterns of inheritance include sex-linked genes (when
alleles are located only on the X or Y chromosome), polygenic inheritance (when several
genes affect one characteristic), incomplete dominance (when an offspring has a pheno-
type between that of its parents), and codominance (when both alleles of a gene are fully
expressed).

© Houghton Mifflin Harcourt Publishing

Company Florida Biology Standards Review
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STANDARD PRACTICE
1 A gardener crossed a plant with red flowers with a plant that had white flowers. The
offspring plants had pink flowers. What is the most likely genetic reason for these
differences in color?
A. codominance

B. recessive pink genes

C. polygenic inheritance

D. incomplete dominance

2 Gregor Mendel crossed a true-breeding tall plant (TT) with a true-breeding short
plant (tt). What are the possible phenotypes for the offspring?
A. all tall B. all short

C. ratio of 1 tall : 1 short D. ratio of 2 tall : 1 short

3 For a certain animal, black fur color is dominant over brown fur color. The pedigree
below shows a cross between two individuals that have black fur.

Black fur
Black fur

Brown fur
Black fur Black fur Black fur Black fur

Which of the following must be true?

A. Both parents are homozygous for the black-fur trait.

B. Both parents are heterozygous for the black-fur trait.

C. The offspring with brown fur has a mutant gene for fur color.

D. One parent is heterozygous and one is homozygous for fur color.

4 The diagram below shows a pedigree.

What can you conclude about the trait shown in this pedigree?
A. The trait is autosomal and dominant.

B. The trait is Y-linked and dominant.

C. The trait is X-linked and dominant.

D. The trait is X-linked and recessive.

5 A population of crabs living on a sandy beach exhibits three colors: dark brown, light
brown, and speckled. The genotypes for these colors are BB for dark brown, bb for
light brown, and Bb for speckled. If a dark brown crab were crossed with a light
brown crab, what would be the probable phenotypic ratio of their offspring?
A. all speckled

B. all dark brown

C. 3 dark brown : 1 light brown

D. 1 dark brown : 2 speckled : 1 light brown

6 In snap peas, yellow flowers (Y) are dominant to white flowers (y).
In the cross YY × Yy, what would be the genotypic ratio of the F1 generation?
A. 1:1

B. 1:2

C. 1:3

D. 3:1
Name Date

SC.912.L.16.3 Biology
Describe the basic process of DNA replication and how it relates to the transmission and
conservation of the genetic information.

STANDARD REVIEW
James Watson and Francis Crick were the first to piece together a model of the structure
of DNA. The discovery of DNA’s structure was important because it clarified how DNA
could serve as genetic material. Watson and Crick determined that a DNA molecule is a
double helix—two strands twisted around each other, like a winding staircase. As shown
below, each strand is made of linked nucleotides. Nucleotides are the subunits that make
up DNA. Each nucleotide is made of three parts: a phosphate group, a five-carbon sugar
molecule, and a nitrogen-containing base. The five-carbon sugar in DNA nucleotides is
called deoxyribose, from which DNA gets its full name, deoxyribonucleic acid.

Phosphate
Basegroup
P

Sugar

Nucleotide

While the sugar molecule and the phosphate group are the same for each nucleotide
in a molecule of DNA, the nitrogen base may be any one of four different kinds:
adenine (A), guanine (G), thymine (T), and cytosine (C). An adenine on one strand
always pairs with a thymine on the opposite strand, and a guanine on one strand
always pairs with a cytosine on the opposite strand.

© Houghton Mifflin Harcourt Publishing

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 DNA
helicase
DNA New DNA
polymerases Old DNA

Replication fork

Old DNA

New DNA

The process of making a copy of DNA is called DNA replication. DNA replication is
summarized in the figure below. First, the two original strands separate. Then, DNA
polymerases add complementary nucleotides to each strand. Because of the strictness of
base-pairing rules, the result is always the formation of two DNA molecules that are
identical to the original DNA molecule.
STANDARD PRACTICE
1 The diagram below represents an incomplete model of the DNA molecule.

G C A A T
T

G A A
A T T G
TA

C
C

C G G C G C

Even though the model is not complete, what pattern is evident in this representation
of the DNA molecule?
A. Every third base pair has a mutation.

B. Guanine is always paired with cytosine.

C. The model has a repeating base sequence.

D. There are two sugars between each phosphate.

2 James Watson and Francis Crick built a model showing that the structure of
DNA is like a twisted ladder called a double helix. What purpose does the
double helix structure serve?
A. The double helix shows that one strand of the DNA ladder is inherited from
each parent.
B. The sugars and phosphates that make up the sides of the DNA ladder twist
and curve to conserve space in the cell.
C. The double helix structure of DNA is a random occurrence, and DNA could
just as easily be a straight, single-strand molecule.
D. The pattern of complimentary bases on each side of the DNA ladder ensures
that exact copies of the DNA are made during replication.
3 DNA is composed of strands of nucleotides that pair in regular patterns and are held
together by the forces shown in the diagram below.
Purines
Adenine (A)Guanine (G)
NH2 C O

Nitrogen base C N
C
C N
Phosphate P group N HN
CH CH
HC C NH2 C N C NH
N NH

Pyrimidines
Thymine (T)Cytosine (C)
Sugar (deoxyribose) O
NH2
C
C
HN C CH3
NCH
P O C N CH H
OC CH
P NH
P
P P
P
P
P
P P
P P
P P
P
P
P
P P
P
P
P
P
P
P P
P P P
P

P P

P
P
P

What forces, represented by dotted lines, hold together the two strands of DNA
shown in the diagram above?
A. ionic bonds

B. covalent bonds

C. hydrogen bonds

D. carbon-carbon bonds

4 The sequence of bases on one strand of a DNA molecule is AGCCTAG. After

replication of the strand of DNA, what is the sequence of nitrogen bases on the
complementary strand?
A. AGCCTAG

B. CTAGGCA

C. GATCCGA

D. TCGGATC
Name Date

SC.912.L.16.4 Biology
Explain how mutations in the DNA sequence may or may not result in phenotypic change.
Explain how mutations in gametes may result in phenotypic changes in offspring.

STANDARD REVIEW
Although changes in an organism’s hereditary information are relatively rare, they
can occur. A change in the DNA of a gene is called a mutation. Mutations in
gametes can be passed on to offspring of the affected individual, but mutations in
body cells affect only the individual in which they occur.
Mutations that move an entire gene to a new location are called gene rearrangements.
Changes in a gene’s position often disrupt the gene’s function because the gene is exposed
to new regulatory controls in its new location.
Mutations that change a gene are called gene alterations. Gene alterations usually
result in the placement of the wrong amino acid during protein assembly. This error
will usually disrupt a protein’s function. In a point mutation, a single nucleotide
changes. In an
insertion mutation, a sizable length of DNA is inserted into a gene. In a deletion mutation,
segments of a gene are lost, often during meiosis. In a duplication mutation, a
chromosome fragment attaches to its homologous chromosome, which will then carry
two copies of a certain set of genes. Another type of mutation is an inversion
mutation, in which the chromosome piece reattaches to the original chromosome but
in a reverse orientation. If the piece reattaches to a nonhomologous chromosome, a
translocation mutation results.
Because the genetic message is read as a series of triplet nucleotides, insertions and
deletions of one or two nucleotides can upset the triplet groupings. Imagine deleting the
letter C from the sentence “THE CAT ATE.” Keeping the triplet groupings, the message
would read “THE ATA TE,” which is meaningless. A mutation that causes a gene to be
read in the wrong three-nucleotide sequence is called a frameshift mutation.

© Houghton Mifflin Harcourt Publishing

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71
STANDARD PRACTICE
1 Errors sometimes happen during DNA replication. If a DNA nucleotide that contains
guanine is accidentally substituted for a DNA nucleotide that contains thymine,
which of the following will always happen because of the error in DNA replication?
A. The cell will die when it divides.

B. The cell will become a rapidly dividing cancer cell.

C. The cell will produce mRNA with a mutated nucleotide sequence.

D. The cell will have a better chance of surviving under different conditions.

2 Mutations can occur during mitosis, which produces body cells, and meiosis, which
produces gametes. Which of the following statements about mutations is true?
A. Mutations in the DNA of body cells cannot affect the individual in which they
happen.
B. A mutation in the DNA of a body cell can cause the cell to produce a
protein that does not function.
C. A mutation in the DNA of a gamete affects the body cells of the individual that
produced the gamete.
D. Mutations in the DNA of body cells can cause the offspring to produce
a protein that does not function.

3 Inheriting two copies of a recessive allele causes an individual to die before reaching
sexual maturity. How is it that this recessive allele can be passed on from one
generation to the next?
A. Two homozygous dominant parents will not give the recessive allele to their off-
spring, so all will reach sexual maturity.
B. Homozygous dominant individuals usually live past sexual maturity to pass on
the dominant allele to their offspring.
C. Heterozygous individuals can pass on the recessive allele because they do not die
from having only one copy of the allele.
D. Two heterozygous parents who have reached sexual maturity will not give the
recessive allele to their offspring, so all will reach sexual maturity.
4 A mutation in the DNA that produced the strand of messenger RNA shown in
the chart below produced a new strand of mutant messenger RNA.

CODON AND ANTICODON PAIRING

Transfer RNA UGA CUG CAG CUU
Messenger RNA ACU GAC GUC GAA

If the mutation was a deletion, which strand of the following sequences would best
represent the resulting mutant messenger RNA?
A. ACGGACGUCGAA

B. ACUACGUCGAA

C. ACUGACGUCCAA

D. ACUGCACGUCGAA

5 Most mutations involve a misplacement of a nucleotide on a DNA segment. Which

of the following is not a possible result of a mutation?
A. The mutation will be passed on to the next generation.

B. The mutation will cause immediate death of the individual.

C. The gene that contains the mutation will be expressed in a new way.

D. The gene that contains the mutation will be expressed in the same way as
before the mutation.
Name Date

SC.912.L.16.5 Biology
Explain the basic processes of transcription and translation, and how they result in the
expression of genes.

STANDARD REVIEW
Traits, such as eye color, are determined by proteins that are built according to instructions
coded in DNA. Recall that proteins have many functions, including acting as enzymes and
cell membrane channels. Proteins, however, are not built directly from DNA. Ribonucleic
acid is also involved.
Like DNA, ribonucleic acid is a nucleic acid—a molecule made of nucleotides linked
together. RNA differs from DNA in three ways. First, RNA consists of a single strand of
nucleotides instead of the two strands found in DNA. Second, RNA nucleotides contain
the five-carbon sugar ribose rather than the sugar deoxyribose, which is found in DNA
nucleotides. Ribose contains one more oxygen atom than deoxyribose contains. And third,
in addition to the A, G, and C nitrogen bases found in DNA, RNA nucleotides can have
a nitrogen base called uracil—abbreviated as U. No thymine (T) bases are found in RNA.
Like thymine, uracil is complementary to adenine whenever RNA base-pairs with another
nucleic acid.
A gene’s instructions for making a protein are coded in the sequence of nucleotides in
the gene. The instructions for making a protein are transferred from a gene to an RNA
mol- ecule (called messenger RNA) in a process called transcription. Cells then use
two different types of RNA (transfer RNA and ribosomal RNA) to read the instructions
on the messenger RNA molecule and put together the amino acids that make up the
protein in
a process called translation. The entire process by which proteins are made based on the
information encoded in DNA is called gene expression, or protein synthesis. This process is
summarized in the figure below.

Nucleus

DNA
Cytoplasm

Transcription
RNA

RNA
Translation
Protein

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STANDARD PRACTICE
1 Which of the following identifies the process of forming a nucleic acid by using
another molecule as a template and identifies its most common form?
A. translation—the synthesis of RNA using one strand of DNA

B. transcription—the synthesis of RNA using one strand of DNA

C. replication—the synthesis of proteins using one strand of RNA

D. protein synthesis—the synthesis of proteins using one strand of DNA

2 What would most likely happen if tRNA malfunctioned during the assembly of a
protein molecule?
A. Amino acids would no longer be transported into the cell.

B. Mutations would happen in the segment of mRNA as it formed.

C. The wrong amino acids would be added to the new protein molecule.

D. Bonds between the amino acids would no longer form inside the ribosome.

3 The diagram below shows one process that occurs during gene expression.

R R R R R R R R
DNA

C T T C
AGAA

G A
UC

mRNA
R R R R

What process does the diagram represent?

A. DNA replication

B. RNA replication

C. transcription

D. translation
4 Which of the following statements best describes the process of gene expression?
A. Messenger, transfer, and ribosomal RNA transcribe information onto a
cell’s DNA.
B. The information in DNA is transcribed to RNA and then transcribed to
amino acids.
C. The information in DNA is transcribed to RNA and then translated to
make specific proteins.
D. The information in DNA is translated by messenger RNA and then translated
to make ribosomal RNA.

5 What DNA sequence complements the messenger RNA

sequence ACUGACGUCGAA?
A. ACTGACGTCGAA

B. ACUGACGUCGAA

C. TGACTGCAGCTT

D. UGACUGCAGCUU

6 Which of the following statements best describes the relationship between genes and
their outcomes?
A. Every gene influences a single trait.

B. Similar genes produce the same outcome in every individual.

C. Every gene can be linked to one specific, predictable outcome.

D. The outcome of a gene is affected by the environment of the cells and the timing
of gene expression.
Name Date

SC.912.L.16.8 Biology
Explain the relationship between mutation, cell cycle, and uncontrolled cell growth
potentially resulting in cancer.

STANDARD REVIEW
Just as traffic lights control the flow of traffic, cells have a system that controls the phases
of the cell cycle. Cells have a set of “red light–green light” switches that are regulated by
feedback information from the cell. The cell cycle has key checkpoints (inspection points)
at which feedback signals from the cell can trigger the next phase of the cell cycle (green
light). Other feedback signals can delay the next phase to allow for completion of the
current phase (yellow or red light). The cell cycle in eukaryotes is controlled by many
proteins.
Certain genes contain the information necessary to make the proteins that regulate cell
growth and division. If one of these genes is mutated, the protein may not function, and
regulation of cell growth and division can be disrupted. Cancer, the uncontrolled growth
of cells, may result. Cancer is essentially a disorder of cell division. Cancer cells do not
respond normally to the body’s control mechanisms.
Some mutations cause cancer by overproducing growth-promoting molecules, thus speed-
ing up the cell cycle. Others cause cancer by inactivating the control proteins that normally
act to slow or stop the cell cycle.

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STANDARD PRACTICE
1 Some genes code for the proteins that regulate cell growth and division. Which of the
following are caused by uncontrolled, abnormal cell division?
A. cancers

B. mutagens

C. oncogenes

D. transposons

2 Cancer is often characterized by tumors. Which would most likely trigger the
formation of a tumor?
A. a parasite that both lived and reproduced within the human body

B. a mutation in a gene that codes for a protein regulating cell division

C. a change in the DNA sequence of a gene that codes for skin coloration

D. a bacterial infection that caused inflammation and swelling in body tissues

3 Some cancers are caused by mutations that stop certain proteins from working.
The inactivation of what kind of protein could lead to cancer?
A. one that sped up the cell cycle

B. one that slowed down the cell cycle

C. one that acted as a growth-promoting molecule

D. one that responded to growth-promoting molecules

4 Cancer cells are body cells that do not function properly, leading to abnormal growth.
Which process does not function normally in cancer cells?
A. osmosis

B. cell cycle

C. photosynthesis

D. cellular respiration
Name Date

SC.912.L.16.9 Biology
Explain how and why the genetic code is universal and is common to almost all organisms.
SC.912.N.1.1

STANDARD REVIEW
Messenger RNA (mRNA) is the form of RNA that carries the instructions for making a
protein from a gene and delivers it to the site of translation. The information is translated
from the language of RNA—nucleotides—to the language of proteins—amino acids. The
RNA instructions are written as a series of three-nucleotide sequences on the mRNA
called codons. Each codon along the mRNA strand corresponds to an amino acid or
signifies a start or stop signal for translation.
The chart below shows the genetic code—the amino acids and “start” and “stop” signals
that are coded for by each of the possible 64 mRNA codons.
With few exceptions, the genetic code is the same in all organisms. For example, the codon
GUC codes for the amino acid valine in bacteria, in eagles, in plants, and in your own cells.
For this reason, the genetic code is often described as being nearly universal. It appears
that all life-forms have a common evolutionary ancestor with a single genetic code. Some
exceptions include the ways cell organelles that contain DNA (such as mitochondria and
chloroplasts) and a few microscopic protists read “stop” codons.

2 Follow that
1 Find the first row to the
3 Move up or down in
base of the mRNA that box until you match
column that
codon in this the third base of the
matches the
column of the codon with this column
second base of
table.
the codon.
Codons in of the chart.
mRNA
Second base
First base
First Third base
U A G

UGU UGC UCAG

Cysteine
UUU
UCU UA
UUC Phenylalanine Tyrosine UGA–Stop UGG–Tryptophan
UUA UCC U
U UCA Serine UAC
UUG Leucine Stop UCAG
UCG UA CGU CGC CGA CGG
A
CUU CCU Arginine
UAG Histidine
CUC CCC
C CUA Leucine Proline
CCA CAU Glutamine
CUG
AUU CCG CAC UCAG
ACU ACC ACA ACG AAU AAC AAAAsparagine
CAA AAGAGU AGC AGA AGG
Serine
G AUC Isoleucine AUA
AUG–Start Threonine CAG Arginine
A Lysine

GUU GUC GUA GUG GCU GCC GCA GCG Aspartic

GAU GAC GAA acid GGC GGA GGG
GAGGGU UCAG
Alanine Glutamic acid Glycine
G Valine

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STANDARD PRACTICE
1 There are 64 possible mRNA codons that make up the genetic code. Which of the
following is true of the genetic code?
A. Codons can be only three amino acids long.

B. Codons can be one, two, or three bases long.

C. Each codon is linked to only one amino acid.

D. Each amino acid is linked to only one codon.

2 A strand of messenger RNA is attached to a ribosome and is directing protein

synthesis. The next exposed codon of this messenger RNA has the code GAA. It is
most likely to bond with a transfer RNA that has which amino acid?
A. arginine B. aspartic acid

C. glutamic acid D. glutamine

3 Mutations have various effects on the amino acid sequence that determines
protein structure and function. A silent mutation has no effect on the protein’s
function.
Which mutation would result in a silent mutation?
A. UAA to CAA

B. CCA to CCG

C. AUA to AUG

D. GCU to GGU

4 The genetic code is nearly universal. That is, with few exceptions, the same codons
code for the same amino acids in all organisms. What does the near universality of the
genetic code suggest?
A. All life-forms can reproduce with one another.

B. All life-forms have the same number of genes.

C. All life-forms have a common evolutionary ancestor.

D. All life-forms arose about the same time in Earth’s history.

Name Date

SC.912.L.16.10 Biology
Evaluate the impact of biotechnology on the individual, society and the environment,
including medical and ethical issues.
SC.912.N.2.2

STANDARD REVIEW
Biotechnology has both positive and negative effects on individuals, society, and the
environment. One topic of current debate is controversy over the risks and benefits of
genetically modified crops. Today, genetic engineers can add favorable characteristics to
a plant by manipulating the plant’s genes. Genetic engineers can change plants in many
ways, including making crop plants more tolerant to drought conditions and creating
plants that can adapt to different soils, climates, and environmental stresses.
Many people, including influential scientists, have expressed concern that genetically
modified crops (GM crops) might turn out to be dangerous. Scientists, the public, and
regulatory agencies must work together to evaluate the risks and benefits of GM products.
What kind of unforeseen negative effects might “improved” GM crops have? Some food
crops, such as corn and soybeans, have been genetically rendered resistant to glyphosate,
a weed killer that is harmless to humans. Glyphosate, when used on a food crop, will kill
the weeds but will not harm the GM crop, thus increasing food crop yields. Some scientists
are concerned that the use of GM crops and the subsequent use of glyphosate will even-
tually lead to glyphosate-resistant weeds. This will leave farmers with few weed-control
alternatives.
Some GM crops have genes added to improve nutritional character, as was done in rice.
It is important to check that consumers are not allergic to the product of the introduced
gene. For this reason, screening of GM crops for causes of allergy problems is now
routine.
Are GM crops harmful to the environment? Will introduced genes pass from GM crops to
their wild or weedy relatives? This sort of gene flow happens naturally all the time, so this
concern is legitimate. For most crops, no closely related wild plant is around to receive the
gene. The GM gene cannot pass to a nonrelative, because crop plants cannot successfully
reproduce with unrelated species, any more than a cat can breed with a giraffe. There are
wild relatives of corn in Mexico and Guatemala, which frequently exchange genes with
corn crops. Scientists are divided about whether it makes any difference if one of the genes
is a GM gene.
Might pests become resistant to GM toxins? Pests are becoming resistant to GM toxins
just as they have become resistant to the chemical pesticides that are sprayed on crops.
Some argue that because GM crops might select and promote the competition and survival
of pests that are resistant to toxins can mean that GM crops do more harm than good.

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STANDARD PRACTICE
1 Some bacteria—sometimes called “super bugs”—have developed a resistance to
certain antibiotics. How does drug resistance develop in bacteria?
A. Unsanitary conditions allow all kinds of bacteria to breed, including those
that are antibiotic resistant.
B. In the bloodstream, different species of bacteria exchange genes and
become resistant to antibiotics.
C. Mutations in some bacterial genes make the bacteria stronger and better able to
defeat the body’s immune system.
D. In the presence of an antibiotic, bacteria with genes that make them resistant
survive and eventually take over the population.

2 Every human begins as a single, fertilized egg. After about five days, a hollow ball has
formed that contains about 30 specialized cells called stem cells. Embryonic stem cells
can divide endlessly and give rise to every type of tissue in the body. Scientists hope
that someday stem cells will make it possible to repair or replace damaged tissues.
Embryonic stem cells used in research come from eggs that were fertilized in the
laboratory and donated for research. Adult stem cells used in research are found in
adult tissues. The use of embryonic stem cells is controversial because a human
embryo is destroyed to obtain these cells. However, currently adult stem cells cannot
be grown in the lab, and they occur in limited numbers in the body. Currently, large
numbers of stem cells are needed for stem cell therapy. How does this fact affect the
debate about the two sources of stem cells?
A. Embryonic stem cells are readily available but are not thought to be useful for
stem cell therapy.
B. Adult stem cells are rare, and research needs to be done in order to find tech-
niques to harvest more adult stem cells.
C. Embryonic stem cells are rare, and research needs to be done in order to find
techniques to harvest more adult stem cells.
D. Somatic cells are destroyed during the collection of adult stem cells and there
would be too much damage to justify the harvesting of these cells.
3 Malaria is a tropical disease characterized by severe chills, headache, and fever.
Malaria is caused by protists in the genus Plasmodium. The disease is spread from
human to human when mosquitoes bite an infected person and transfer Plasmodium
sporozoites to another person. Hoping to develop a product that prevents malarial
infection, a scientist researches chemicals that kill different types of organisms. How
might the scientist use a chemical to prevent the transmission of malaria from person
to person?
A. The scientist might develop a chemical that kills either the mosquito that
trans- fers the protist or the protist itself.
B. The scientist might develop a chemical that kills all Plasmodium
sporozoites found in water and food sources.
C. The scientist might develop a chemical that kills the protist after it has
been transmitted from one person to another.
D. The scientist might develop a chemical that treats the more severe symptoms of
malaria, such as chills, headache, and fever.

4 Microbiology is the study of microorganisms. How does studying the life cycle of
microorganisms relate to modern medicine?
A. The life cycle of microorganisms can provide important evidence of
environmental degradation.
B. The life cycle of microorganisms can provide clues to how cells in the human
body regulate themselves.
C. Scientists study the life cycle of microorganisms in order to find a way to treat
cancer and other chronic illnesses.
D. Scientists study the life cycle of pathogens in order to find a way to interrupt
the course of disease and the spread of infection.
Name Date

SC.912.L.16.13 Biology
Describe the basic anatomy and physiology of the human reproductive system. Describe
the process of human development from fertilization to birth and major changes that
occur in each trimester of pregnancy.

STANDARD REVIEW
During fertilization, an egg and sperm unite within the mother’s reproductive system.
Development begins upon fertilization, with a single diploid cell from which billions of
other cells arise. The uterus provides protection and nourishment during development.
Human development takes about 9 months—a period known as gestation, or pregnancy.
The 9 months of pregnancy are often divided into three trimesters, or 3-month periods.
For the first 8 weeks of pregnancy, the developing human is called an embryo. From the
eighth week of pregnancy until childbirth, the developing human is called a fetus.
The most crucial events of development occur very early in the first trimester. In the
second week after fertilization—shortly after implantation—the embryo grows rapidly.
Membranes that will protect and nourish it also develop. One of these membranes, the
amnion, encloses and protects the embryo. Another membrane, the chorion, interacts with
the uterus to form the placenta.

The placenta is the structure through which the mother nourishes the embryo. The moth-
er’s blood normally never mixes with the blood of the embryo. Instead, nutrients in the
mother’s blood diffuse through the placenta and are carried to the embryo through blood
vessels in the umbilical cord. The waste products of the embryo also pass through the
placenta into the mother’s blood.
During the second and third trimesters, the fetus grows rapidly as its organs become
functional. By the end of the third trimester, the fetus is able to exist outside the mother’s
body. After about 9 months of development, the fetus leaves the mother’s body in a
process called labor, which usually lasts several hours. During labor, the walls of the uterus
contract, expelling the fetus from the uterus and through the vagina. The placenta and the
umbilical cord are expelled after the baby is born.

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STANDARD PRACTICE
1 The human mother’s body nourishes the growing embryo during pregnancy. Which
structure nourishes the embryo and removes wastes through a network of blood
vessels?
A. ovary

B. placenta

C. uterus

D. zygote

2 The point at which the umbilical cord attaches to the fetus becomes the belly button
after birth. What is the function of the umbilical cord for mother and fetus during
fetal development?
A. Liquids consumed by the mother flow through the umbilical cord and into the
stomach of the fetus.
B. Waste products produced by the fetus travel through the umbilical cord and
out through the mother’s kidneys.
C. Part of any food consumed by the mother passes from the mother’s stomach,
through the umbilical cord, and into the stomach of the fetus.
D. Nutrients from foods digested by the mother pass from her blood to the
fetus through blood vessels in the umbilical cord.
3 The diagram shows a sperm cell.

Which part(s) secrete(s) enzymes that break down the outer layers of the ovum,
allowing the sperm to enter?
A. part A

B. part B

C. part C

D. parts A and C

4 A mature egg is called an ovum and is 75,000 times larger than a sperm cell. What
can you infer from this comparison?
A. The ovum contains more cytoplasm than the sperm.

B. The condition of the ovum is a better predictor of fetal health.

C. The sperm is more likely to reach the ovum due to its small size.

D. The ovum donates more genetic information to the zygote than the sperm.
Name Date

SC.912.L.16.14 Biology
Describe the cell cycle, including the process of mitosis. Explain the role of mitosis
in the formation of new cells and its importance in maintaining chromosome number
during asexual reproduction.

STANDARD REVIEW
The life of a eukaryotic cell is traditionally shown as a cycle, as illustrated in the figure
below. The cell cycle is a repeating sequence of cellular growth and division during the life
of an organism. A cell spends 90 percent of its time in the first three phases of the cycle,
which are collectively called interphase. A cell will enter the last two phases of the cell
cycle only if it is about to divide. The five phases of the cell cycle are summarized below:

1. First growth (G1) phase: During the G1 phase, a cell grows rapidly and carries
out its routine functions. For most organisms, this phase occupies the major
portion of the cell’s life. Cells that are not dividing remain in the G 1 phase.
2. Synthesis (S) phase: A cell’s DNA is copied during this phase. At the end of
this phase, each chromosome consists of two chromatids attached at the
centromere.
3. Second growth (G2) phase: In the G2 phase, preparations are made for the
nucleus to divide. Hollow protein fibers called microtubules are rearranged during
G2 in preparation for mitosis.
4. Mitosis: The process during cell division in which the nucleus of a cell is divided
into two nuclei is called mitosis. Each nucleus ends up with the same number and
kinds of chromosomes as the original cell.
5. Cytokinesis: The process during cell division in which the cytoplasm divides
is called cytokinesis.

Steps of Mitosis
Step 1: Prophase Chromosomes coil up and become visible during prophase. The nuclear
envelope dissolves and a spindle forms.
Step 2: Metaphase During metaphase the chromosomes move to the center of the cell and
line up along the equator. Spindle fibers link the chromatids of each chromosome to
opposite poles.

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Step 3: Anaphase Centromeres divide during anaphase. The two chromatids (now called
chromosomes) move toward opposite poles as the spindle fibers attached to them shorten.
Step 4: Telophase A nuclear envelope forms around the chromosomes at each pole.
Chromosomes, now at opposite poles, uncoil and the spindle dissolves. The spindle fibers
break down and disappear. Mitosis is complete.
Mitosis and cytokinesis produce new cells that are identical to the original cells and allow
organisms to grow, replace damaged tissues, and, in some organisms, reproduce asexually.
STANDARD PRACTICE
1 The following diagrams show four stages of a cell dividing by mitosis.

1 2 3 4
Which list of numbers names the diagrams in the correct sequence as they occur in
the cell cycle?
A. 1, 3, 4, 2 B. 2, 1, 3, 4

C. 2, 4, 3, 1 D. 4, 3, 2, 1

2 The cell cycle is a repeating sequence of cellular growth and division during the life of
an organism. Which of the following is not a true statement concerning cell division
of body cells?
A. Cells divide in a process called meiosis.

B. Cells divide in order to maintain homeostasis.

C. Cells divide when the parent cell gets too big.

D. Cells divide in order to repair themselves when damaged.

3 Construction workers attach ropes and pulleys to wooden timbers on an old
bridge. They use the rope and pulley system like the one in the diagram below to
move the timbers away from each other, in order to dismantle the bridge.

Which stage of mitosis is similar to this way of dismantling a bridge?

A. anaphase

B. metaphase

C. prophase

D. telophase

4 As part of the cell cycle, a cell produces new daughter cells that are identical to the
original cell. During which phase are the two daughter cells physically separated?
A. cytokinesis

B. synthesis phase

C. first growth phase

D. second growth phase

Name Date

SC.912.L.16.16 Biology
Describe the process of meiosis, including independent assortment and crossing over.
Explain how reduction division results in the formation of haploid gametes or spores.

STANDARD REVIEW
Some organisms reproduce by joining gametes to form the first cell of a new individual. The
gametes are haploid—they contain one set of chromosomes. Meiosis is a form of cell division
that halves the number of chromosomes when forming specialized reproductive cells, such as
gametes or spores. Meiosis involves two divisions of the nucleus—meiosis I and meiosis II.
Steps of Meiosis
Before meiosis begins, the DNA in the original cell is replicated. Thus, meiosis starts with
homologous chromosomes. Recall that homologous chromosomes are similar in size,
shape, and genetic content. The stages of meiosis are summarized below:
Step 1: Prophase I The chromosomes condense, and the nuclear envelope breaks down.
Homologous chromosomes pair along their length. Crossing-over occurs when portions of
a chromatid on one homologous chromosome are broken and exchanged with the corre-
sponding chromatid portions of the other homologous chromosome.
Step 2: Metaphase I The pairs of homologous chromosomes are moved by the spindle to
the equator of the cell. The homologous chromosomes remain together.
Step 3: Anaphase I The homologous chromosomes separate. As in mitosis, the chromo-
somes of each pair are pulled to opposite poles of the cell by the spindle fibers. But the
chromatids do not separate at their centromeres—each chromosome is still composed of
two chromatids. The genetic material, however, has recombined.
Step 4: Telophase I Individual chromosomes gather at each of the poles. In most organ-
isms, the cytoplasm divides (cytokinesis), forming two new cells. Both cells or poles con-
tain one chromosome from each pair of homologous chromosomes. Chromosomes do not
replicate between meiosis I and meiosis II.
Step 5: Prophase II A new spindle forms around the chromosomes.
Step 6: Metaphase II The chromosomes line up along the equator and are attached at their
centromeres to spindle fibers.
Step 7: Anaphase II The centromeres divide, and the chromatids (now called
chromosomes) move to opposite poles of the cell.
Step 8: Telophase II A nuclear envelope forms around each set of chromosomes. The
spindle breaks down, and the cell undergoes cytokinesis. The result of meiosis is four haploid
cells.
In humans, each gamete receives one chromosome from each of 23 pairs of homologous
chromosomes. But, which of the two chromosomes that an offspring receives from each of
the 23 pairs is a matter of chance. This random distribution of homologous chromosomes
during meiosis is called independent assortment. Each of the 23 pairs of chromosomes
segregates (separates) independently. Thus, 223 (about 8 million) gametes with different
gene combinations can be produced from one original cell by this mechanism. Crossing-
over adds even more recombination.
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STANDARD PRACTICE
1 Agricultural scientists develop a way to insert a third chromosome into the diploid
body cells of a watermelon plant to create a seedless fruit. How could this extra
chromosome affect watermelon offspring?
A. All gametes produced would have twice the number of chromosomes, so off-
spring would be tetraploid.
B. Because the chromosome was inserted into a diploid body cell, it would not be
passed to offspring through meiosis.
C. The extra chromosome would be passed to offspring through meiosis, so each
generation would produce seedless fruit.
D. With three chromosomes, the watermelon would produce three times as many
offspring as a normal watermelon plant.

2 During meiosis, homologous chromosomes line up next to each other. If one arm of
a chromatid crosses over the arm of another chromatid, what results?
A. the creation of an additional sex cell

B. the independent assortment of genetic material

C. a possible change in the offspring cell’s functionality

D. additional variation in the DNA combination of each sex cell formed

3 Which statement explains why approximately half of an individual’s DNA sequence

comes from each parent?
A. A cell from one parent undergoes meiosis, producing offspring cells that have
both parents’ DNA.
B. A cell from one parent undergoes mitotic cell division, producing offspring cells
that have only half of that parent’s DNA.
C. Cells in the parents undergo meiosis, producing haploid gametes that meet up
during fertilization to produce a diploid individual.
D. Cells in the parents undergo mitosis, producing offspring cells that meet up
during fertilization to produce an individual with half of each parent’s DNA.
4 Crossing-over contributes to the recombination of genetic material in offspring. When
does crossing-over happen during meiosis?
A. when the DNA of the diploid cell is copied

B. when homologous chromosomes move to opposite ends of the dividing cell

C. when spindle fibers move the chromosomes toward the midline of the dividing
cell
D. when homologous chromosomes pair and portions of chromatids break off
and are exchanged
Name Date

SC.912.L.16.17 Biology
Compare and contrast mitosis and meiosis and relate to the processes of sexual
and asexual reproduction and their consequences for genetic variation.

STANDARD REVIEW
Some organisms look exactly like their parents and siblings. Others share traits with
family members but are not identical to them. Some organisms have two parents, while
others have one. The type of reproduction that produces an organism determines how
similar the organism is to its parents and siblings. Reproduction, the process of
producing offspring, can be asexual or sexual.
In asexual reproduction a single parent passes copies of all of its genes to each of its
offspring; there is no fusion of haploid cells such as gametes. Asexual reproduction
involves only mitosis, not meiosis. An individual produced by asexual reproduction is
a clone, an organism that is genetically identical to its parent. Prokaryotes reproduce
by a type of asexual reproduction called binary fission. Many eukaryotes, such as
hydra, also reproduce asexually through a process called budding, in which new
individuals split off from existing ones. Some multicellular eukaryotes undergo
fragmentation, a type of reproduction in which the body breaks into several pieces.
Some or all of these fragments later develop into complete adults when missing parts
are regrown. Vegetative propagation is a similar reproductive method that farmers use
to grow new crops.
In contrast, in sexual reproduction two parents each form reproductive cells through
meiosis that have one-half the number of chromosomes. A diploid mother and father
would give rise to haploid gametes, which join to form diploid offspring. Because both
parents contribute genetic material, the offspring have traits of both parents but are not
exactly like either parent. Sexual reproduction, with the formation of haploid cells, occurs
in eukaryotic organisms, including humans.
Asexual reproduction is the simplest and most primitive method of reproduction. In a
stable environment, asexual reproduction allows organisms to produce many offspring
in a short period of time, without using energy to produce gametes or to find a mate.
However, the DNA of these organisms varies little between individuals. This may be a
disadvantage in a changing environment because a population of organisms may not
be able to adapt to a new environment.
Sexual reproduction, on the other hand, provides a powerful means of quickly making
different combinations of genes among individuals. Such genetic diversity is the raw
material for evolution.

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STANDARD PRACTICE
1 Konesha’s eyes are brown, just like her mother’s eyes. Konesha has long fingers like
her father, but her mother’s fingers are much shorter. What is the most likely reason
why Konesha’s appearance is similar to and different from that of her parents?
A. All of her traits depended upon pure chance.

B. Her eye color is an inherited trait, but finger length is a trait that evolved in
Konesha.
C. She inherited some traits, such as eye color, from her mother and others, such as
finger length, from her father.
D. She inherited her eye color from her mother but grew longer fingers because she
had better nutrition as an infant.

2 Imagine that sex cells divided by mitosis instead of meiosis. What would then be the
result of fertilization of an ovum by a sperm cell?
A. Fertilization would result in the formation of two identical cells.

B. The new individual would be identical to only one of the parents.

C. Fertilization would cause crossing-over and recombination of genes.

D. Cells of the new individual would have double the necessary number of
chromosomes.

3 Variation within organisms within a species increases the chance that a species
will survive changing conditions. What kind of reproduction produces the most
variation within a species?
A. budding B. parthenogenesis

C. sexual reproduction D. asexual reproduction

4 Some 2,500 varieties of apples are grown in the United States, mainly by
vegetative propagation. Which of the following are methods of producing
apples in this way?
A. grafting or budding

B. planting bulbs or corms

C. hand pollination or bee pollination

D. growing plants from seeds that germinate indoors

5 A cheetah is a multicellular organism. A paramecium is a unicellular organism. How
do these two organisms differ in terms of how they produce offspring?
A. The cheetah uses sexual reproduction, while the paramecium uses meiosis.

B. The cheetah uses binary fission, while the paramecium uses sexual reproduction.

C. The cheetah uses asexual reproduction, while the paramecium uses binary
fission.
D. The cheetah uses sexual reproduction, while the paramecium uses asexual
reproduction.
Name Date

SC.912.L.17.2 Biology
Explain the general distribution of life in aquatic systems as a function of
chemistry, geography, light, depth, salinity, and temperature.
MA.912.S.1.2

STANDARD REVIEW
Aquatic systems include freshwater and saltwater communities. Each differs in chemistry,
geography, light, depth, salinity, and temperature and thus the kinds of life it supports.
Freshwater Communities Ponds and lakes have three zones in which organisms live. The
littoral zone is a shallow zone near the shore. Here, aquatic plants live along with various
predatory insects, amphibians, and small fish. The limnetic zone refers to the area that
is farther away from the shore but close to the surface. It is inhabited by floating
algae, zooplankton, and fish. The profundal zone is a deep-water zone that is below
the limits of effective light penetration. Numerous bacteria and wormlike organisms
eat debris on the lake’s bottom, releasing large amounts of nutrients.
Wetland Communities Swamps, marshes, bogs, and other communities that are
covered with a layer of water are called wetlands. Wetlands typically are covered
with a variety of water-tolerant plants, called hydrophytes (“water plants”). Marsh
grasses and cattails are hydrophytes. Wetlands are dynamic communities that support
a diverse array of inverte- brates, birds, and other animals. Wetlands are among the
most productive ecosystems on Earth.
Shallow Ocean Water Communities The zone of shallow water is small in area, but com-
pared with other parts of the ocean, it is inhabited by large numbers of species. The
seashore between high and low tide, called the intertidal zone, is home to many species of
marine invertebrates. Coral reef communities, the world’s most diverse, occur in shallow
tropical waters. The world’s great fisheries are located in the coastal zones of cooler waters,
where nutrients washed out from land support huge numbers of fishes.
Communities at the Surface of the Open Sea Drifting freely in the upper waters of the
ocean is a diverse community of plankton, composed of bacteria, algae, fish larvae, and
many small invertebrate animals. Fishes, whales, and invertebrates such as jellyfishes feed
on plankton. And larger fishes and birds, in turn, feed on some of these animals. Photo-
synthetic plankton (algae such as diatoms and some bacteria) that form the base of this
food chain account for about 40 percent of all the photosynthesis that takes place on
Earth. Because light penetrates water only to the depth of about 100 m (328 ft), this rich
community is confined to the ocean’s surface.
Communities at the Ocean Depths In the deepest waters of the sea, the marine
community lives in total darkness, in deep cold, and under great pressure. Despite what
seem like hostile conditions, the deep ocean supports a diverse community of
invertebrates and fishes. This includes great squids and angler fishes that attract prey with
projections from their head that emit light. On the ocean floor, at an average depth of
more than 3 km
(1.9 mi), researchers have also found an abundance of species.

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STANDARD PRACTICE
1 Zebra mussels are tiny clam-like organisms that strain plankton out of lake water.
They attach to piers, boat hulls, pipes, and any other available surface. They prefer
attaching to surfaces in water that is 2 to 7 feet deep but have been found at depths of
180 feet in water temperatures ranging from 7˚C to 32˚C. They live mainly in
freshwater with salinities (salt concentrations) of 0.21 to 1.47 parts per thousand
(ppt), but they can live in estuaries with salinities up to 13.40 ppt. Given what is
known about the diet of zebra mussels, which variables influence their fundamental
niche?
A. nearby boats, fish larvae, water salinity, availability of plankton

B. water depth, water clarity, availability of plankton, water temperature,

water salinity
C. water clarity, availability of native mussel species, water temperature, water
salinity
D. availability of surfaces for attachment, water depth, water salinity, availability of
plankton

2 Acid rains form when air pollution combines with moisture in the atmosphere
and falls to Earth as precipitation that has a low pH. Which of the following
effects cannot be attributed to acid rain?
A. an increase in CO2 in Earth’s atmosphere
B. damage to historic buildings and monuments

C. death of aquatic organisms in lakes and streams

D. damage to trees and a decline of forest communities

3 Constance made the table below during a field investigation on ocean life.
Characteristics of Several Ocean Zones
Zones Description
Intertidal Air, sun, and water exposure; crashing waves
Neritic Water depth less than 200 m; lots of sunlight; rela-
tively warm water
Benthic Very deep water; no light; cold except near thermal
vents that emit heat and chemicals
Based on Constance’s table, which of the following is a valid conclusion?
A. Organisms in the intertidal zone must be able to withstand very cold water.
B. There are no producers in the benthic zone that rely on photosynthesis.

C. Organisms in the benthic zone must be able to tolerate occasional air exposure.

D. The warm water and abundant sunlight in the neritic zone limits the
plankton population.

4 A scientist set up two glass fish tanks. She put 5 L of water, a small fish, and several
plants in each tank. She then sealed the tops of both tanks so that no air could leave
or enter. Tank A was placed in a bright room. Tank B was placed in a dark room.
After 45 hours, the fish in the tank that was kept in darkness died. The fish in the
brightly lit tank remained healthy for more than 96 hours.

Based on the results of the experiment described above, what conclusion can you
draw about the relationship between the aquarium conditions and the organisms that
live within the aquarium?
A. Fish cannot survive in an overcrowded aquarium.

B. Fish cannot survive in an aquarium that does not contain plants.

C. Fish cannot survive in a plant-filled aquarium kept in darkness for 45 hours.

D. Fish cannot survive in a plant-filled aquarium that has been sealed so that no air
can leave or enter.
Name Date

SC.912.L.17.4 Biology
Describe changes in ecosystems resulting from seasonal variations, climate change and
succession.

STANDARD REVIEW
An ecosystem, or ecological system, consists of a community and all the physical aspects
of its habitat, such as the soil, water, and weather. Earth’s ecosystems may seem stable, but
they are not static. They change seasonally, they can change suddenly, and they can even
change over time. Climate change is one way that ecosystems can change. They can also
change through a regular, progressive process called succession.
When a volcano forms a new island, a glacier recedes and exposes bare rock, or a fire burns
all of the vegetation in an area, a new habitat is created. This change sets off a process
of colonization and ecosystem development. The first organisms to live in a new habitat
where soil is present tend to be small, fast-growing plants, called pioneer species. They may
make the ground more hospitable for other species. Later waves of plant immigrants may
then outcompete and replace the pioneer species.
Succession is the somewhat regular progression of species replacement. Succession that
occurs where life has not existed before is called primary succession. Succession that
occurs in areas where there has been previous growth, such as in abandoned fields or forest
clearings, is called secondary succession. It was once thought that the stages of succession
were predictable and that succession always led to the same final community of
organisms within any particular ecosystem. Ecologists now recognize that initial
conditions and chance play roles in the process of succession. For example, if two species
are in competition, a sudden change in the climate may favor the success of one species
over the other. For this reason, no two successions are alike.

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STANDARD PRACTICE
1 After fires destroyed 793,000 acres of aspen and pine forest in Yellowstone National
Park in the unusually dry summer of 1988, biologists were able to study the long-term
effects of fire on an ecosystem. The biologists found that the soil after the fire was
more fertile and soon gave rise to small plants and new pine trees. What ecological
process were the biologists observing?
A. biodiversity

B. equilibrium

C. food web energy flow

D. succession

2 Forest fires can burn down all of the trees and plants living in an area. However, new
growth can spring from the decaying organic matter left behind. What is the gradual,
sequential regrowth of a community of species after a forest fire?
A. adaptation

B. pioneer succession

C. primary succession

D. secondary succession

3 The tropical rain forests have been called the lungs of the planet. They take huge
quantities of carbon dioxide from the atmosphere during photosynthesis and give off
large quantities of oxygen. Vast tracts of tropical rain forests are being cleared for
farms by people cutting down and burning the trees. Which of the following is the
best hypothesis about the effect that rain forest destruction may have on atmospheric
carbon dioxide levels?
A. Rain forest destruction will not influence overall carbon dioxide levels, because it
will affect only areas around the equator.
B. Any carbon dioxide added to the atmosphere because of rain forest destruction
will fall into the oceans and be used in building coral reefs.
C. Cutting down rain forest trees will decrease atmospheric carbon dioxide levels,
because plants give off carbon dioxide as a result of cellular respiration.
D. Cutting down trees, which take carbon dioxide from the atmosphere, and
burning the trees, which adds carbon dioxide to the atmosphere, will increase
atmospheric carbon dioxide levels.
4 The panels in the diagram below show how an area progresses over time.

What process is illustrated by the diagram?

A. pioneer speciation B. primary succession

C. secondary succession D. ecosystem boundaries

5 A biologist compares how different ecosystems undergo succession. She divides the
series of events that happen during primary succession and secondary succession into
three different stages. Which feature might be used to distinguish secondary
succession from primary succession?
A. the lack of pioneer species in the second stage

B. the presence of pioneer species in the final stage

C. the presence of trees and large plants in the final stage

D. the presence of soil and organic matter in the first stage

6 A nursery owner wants Easter lilies to bloom in the spring and poinsettias to bloom
during the December holidays. Which plant response would the owner manipulate to
make sure each plant bloomed for the appropriate season?
A. photoperiodism, a response to the length of days and nights

B. heliotropism, a response to the position of the Sun in the sky

C. phototropism, a response to the direction from which light is coming

D. dormancy, in which a seed remains inactive until conditions are suitable for
growth
Name Date

SC.912.L.17.5 Biology
Analyze how population size is determined by births, deaths, immigration, emigration, and
limiting factors (biotic and abiotic) that determine carrying capacity.
MA.912.S.3.2

STANDARD REVIEW
A population consists of all the individuals of a species that live together in one place at
one time. Every population tends to grow because individuals tend to have multiple
offspring over their lifetime. A population grows when more individuals are born than die
in a given period. But eventually, limited resources in an environment limit the growth of a
population.
When population size is plotted against time on a graph, the population growth curve
resembles a J-shaped curve and is called an exponential growth curve. An exponential
growth curve is a curve in which the rate of population growth stays the same, and as a
result, the population size increases steadily.
However, populations do not usually grow unchecked. Their growth is limited by
predators, disease, and the availability of resources. Eventually, growth slows, and the
population may stabilize. The population size that an environment can sustain is called the
carrying capacity.
As a population grows, limited resources (that is, resources in short supply) eventually
become depleted. When this happens, the growth of the population slows. The population
model can be adjusted to account for the effect of limited resources, such as food and
water. These resources are called density-dependent factors because the rate at which they
become depleted depends upon the population density of the population that uses them.
The population model that takes into account the declining resources available to popula-
tions is called the logistic model of population growth, after the mathematical form of the
equation. The logistic model is a population model in which exponential growth is limited
by a density-dependent factor. Unlike the simple model, the logistic model assumes that
birth and death rates vary with population size. When a population is below carrying
capacity, the growth rate is rapid. However, as the population approaches the carrying
capacity, death rates begin to rise and birthrates begin to decline. Competition for food,
shelter, mates, and limited resources tends to increase as a population approaches its
carrying capacity. The accumulation of wastes also increases. As a result, the rate of
growth slows. The population eventually stops growing when the death rate equals the
birthrate.

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STANDARD PRACTICE
1 Researchers have found that a local squirrel population fluctuates from year to year,
increasing one year and decreasing the next. Which of the following factors would
cause the squirrel population to grow?
A. the birth rate is equal to the death rate

B. emigration is greater than immigration

C. the death rate is higher than the birth rate

D. the birth rate is greater than the death rate

2 Your class has been observing the population growth of a species of Paramecium, a
single-celled organism, for 18 days. Your data are shown in the graph below. Food
was occasionally added to the test tube in which the paramecia were grown.
Number ofmL
per

100
Paramecium

50

0
3691215 18
Days

Look at the graph above. What is the carrying capacity of the test-tube environment
as long as food is added?
A. about 10 paramecia

B. about 50 paramecia

C. about 65 paramecia

D. about 100 paramecia

3 Examine the table below.

Year Human Population Growth (billions)

1804 1
1927 2
1960 3
1975 4
1987 5
1999 6

Which graph most closely represents human population growth based on the data
provided above?

Population
Population

A. B.
Population

Population

C. D.

4 All populations fluctuate in size. Which of the following is a nonenvironmental factor

that might cause a population to be reduced in size?
A. decreased predation
B. increased competition

C. emergence of disease resistance

D. increased availability of a food source

5 Which is a way in which the Central Arizona Project, which diverts water from
the Colorado River, could have contributed to population growth in southern
Arizona?
A. It provided water for neighborhood lawns.

B. It enabled better flood control during rains in the desert.

C. It provided an opportunity for recreational fishing and swimming.

D. It provided a reliable supply of water for drinking and agricultural irrigation.

6 The population size that an environment can sustain is called the carrying capacity.
Which of the following factors would not decrease the carrying capacity of a pond
environment?
A. drought

B. flooding

C. food shortages

D. unusually low temperatures

Name Date

SC.912.L.17.8 Biology
Recognize the consequences of the losses of biodiversity due to catastrophic events,
climate changes, human activity, and the introduction of invasive, non-native species.

STANDARD REVIEW
The variety of organisms, their genetic differences, and the communities and ecosystems in
which they occur is termed biodiversity. Biodiversity is a measure of both the number of
different species in a community (species richness) and the relative numbers of each of the
species (species diversity). Some of the most diverse communities are those living in tropi-
cal rainforests.
Over the last 50 years, about half of the world’s tropical rainforests have been burned to
make pasture and farmland or have been cut for timber. Many thousands of square miles
more will be destroyed this year. The people responsible, often poor farmers, view the
forest lands as a resource to be developed, much as Americans viewed North American
forests a century ago.
The problem is that as the rainforests disappear, so do their inhabitants. No one knows
how many species are being lost. To find out, scientists carefully catalogue all of the
residents of one small segment of forest and then extrapolate their data. That is, scientists
use what they know to predict what they don’t know. The resulting estimates vary widely,
but it is clear Earth is losing many species. Some 10 percent of well-known species teeter
on the brink of extinction. Worst-case estimates are that we will lose up to one-fifth of the
world’s species of plants and animals—about 1 million species—during the next 50 years.
An extinction of this size has not occurred in at least 65 million years, since the end of the
age of dinosaurs.
The tragedy of extinction is that as species disappear, so do our chances to learn
about them and their possible benefits. This situation is comparable to burning a
library before reading the books—we lose forever the knowledge we might have
gained. Also, experi- ments have clearly demonstrated that an ecosystem’s
biodiversity and productivity are related. That is, increased species richness leads to
greater productivity.

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STANDARD PRACTICE
1 Imagine that a city located in a desert environment has grown significantly over the
last few decades. Which statement best describes how the growth of the metropolitan
city would impact the desert environment in the area?
A. Biodiversity would decrease in the area but increase in the desert beyond the city.

B. By destroying habitat to build homes and highways, the growth of the city would
decrease the biodiversity in the desert.
C. The growth of the city would have little impact on the environment, because few
animals likely lived there before growth happened.
D. By bringing in water, the growth of the city would improve the entire desert envi-
ronment and increase the biodiversity.

2 As an increasing amount of carbon dioxide is sent into the atmosphere by burning

fossil fuels, the oceans absorb more and more of the excess carbon. Some of the
carbon reacts chemically in seawater to form an acid. The more carbon the ocean
takes in, the more acidic the water becomes. If the water becomes too acidic, tiny
organisms that make up plankton may not be able to make protective shells. One of
the areas that would be greatly affected is the Antarctic. What effect, if any, might
this harm to plankton have on an Antarctic marine food web?
A. It would have no effect, because the organisms in plankton include algae, which
do not have shells.
B. It would be beneficial, because consumers in the third trophic level could
more easily eat zooplankton that do not have protective shells.
C. Damage to the Antarctic ecosystem would be extensive but could be
repaired as new plankton drifts south to replace the plankton that died off.
D. The dying off of these tiny organisms would be disastrous, because zooplankton
is a major source of food for small and large marine organisms.
3 Biologists speculate that in the mid-1980s, a cargo ship from Eastern Europe
dumped ballast water containing a tiny clam-like organism called the zebra mussel
into the Great Lakes. The zebra mussel is native to waters in Eastern Europe but
had never lived in North America. The zebra mussels began to attach to piers, boat
hulls, pipes, and any other available surface. Conditions in the Great Lakes were
ideal for these filter feeders, which strain plankton out of lake water. Plankton is a
food resource for other small aquatic animals and fish larvae. Zebra mussels can
strain food from about one liter of water per day. These invaders thrived and spread
throughout the Great Lakes. They now threaten other North American waterways.
The presence of large quantities of plankton decreases water clarity. Since zebra
mussels were introduced, the clarity of Lake Erie’s water has increased 600%.
What does this most likely indicate about zebra mussels?
A. In clear water, it is easy to catch zebra mussels.

B. Zebra mussels could be used to solve water pollution problems.

C. There is not enough data given to infer a link between zebra mussels and water
clarity.
D. Zebra mussels are such efficient feeders that they have greatly reduced the
amount of plankton in Lake Erie.

4 Imagine that a nonnative bird species appears in an ecosystem. Which of the

following will not be a likely change in the ecosystem?
A. Native bird species will immediately migrate to another area.

B. Bird predator species will temporarily have more available prey.

C. Each food chain in the ecosystem will adjust over time to include the new species
of bird.
D. Birds that share the same niche of the new species will have more competition
for food resources.
Name Date

SC.912.L.17.9 Biology
Use a food web to identify and distinguish producers, consumers, and decomposers.
Explain the pathway of energy transfer through trophic levels and the reduction of
available energy at successive trophic levels.
LA.910.2.2.3

STANDARD REVIEW
Everything that organisms do in ecosystems—running, breathing, burrowing, growing—
requires energy. The flow of energy is the most important factor that controls what kinds
of organisms live in an ecosystem and how many organisms the ecosystem can support.
Most life on Earth depends on photosynthetic organisms, which capture some of the Sun’s
light energy and store it as chemical energy in organic molecules. These organic com-
pounds are what we call food. The rate at which organic material is produced by photosyn-
thetic organisms in an ecosystem is called primary productivity. Primary productivity
determines the amount of energy available in an ecosystem. Most organisms in an ecosys-
tem can be thought of as chemical machines driven by the energy captured in photosyn-
thesis. Organisms that first capture energy, the producers, include plants, some kinds of
bacteria, and algae. Producers make energy-storing molecules. All other organisms in an
ecosystem are consumers. Consumers are those organisms that consume plants or other
organisms to obtain the energy necessary to build their molecules.
Ecologists study how energy moves through an ecosystem by assigning organisms in that
ecosystem to a specific level, called a trophic level, in a graphic organizer based on the
organism’s source of energy. Energy moves from one trophic level to another. The path of
energy through the trophic levels of an ecosystem is called a food chain. However, in most
ecosystems, energy does not follow simple straight paths because individual animals often
feed at several trophic levels. This creates a complicated, interconnected group of food
chains called a food web.
The lowest trophic level of any ecosystem is occupied by the producers, such as
plants, al- gae, and bacteria. Producers use the energy of the Sun to build energy-rich
carbohydrates. Many producers also absorb nitrogen gas and other key substances
from the environment and incorporate them into their biological molecules.
At the second trophic level are herbivores, animals that eat plants or other primary pro-
ducers. They are the primary consumers. Cows and horses are herbivores, as are caterpil-
lars and some ducks. At the third trophic level are secondary consumers, animals that eat
other animals. These animals are called carnivores. Tigers, wolves, and snakes are carni-
vores. Some animals, such as bears, are both herbivores and carnivores; they are called
omnivores. Many ecosystems contain a fourth trophic level composed of those carnivores
that consume other carnivores. They are called tertiary consumers, or top carnivores.
In every ecosystem there is a special class of consumers called detrivores, which include
worms and fungal and bacterial decomposers. Detrivores are organisms that obtain their
energy from the organic wastes and dead bodies that are produced at all trophic levels.

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110 Florida Biology Standards Review
Name Date

SC.912.L.17.9 Biology
STANDARD PRACTICE
1 The Yellowstone area contains organisms representing all trophic levels, including
plants, algae, moss, fungi, blue jays, fish, and grizzly bears. Which sequence best
represents the transfer of energy through Yellowstone trophic levels?
A. fungi to moss to algae to fish

B. blue jay to moss to fungi to plant

C. algae to fish to grizzly bear to fungi

D. plant to blue jay to algae to grizzly bear

2 The food web below represents the interactions between organisms in a salt marsh
ecosystem and organisms in an old field ecosystem.

FOOD WEB
Coyote Hawk

Shrew
Mouse Duck Heron

Fish
Grasshopper Brine shrimp

Plants Algae and phytoplankton Crab

Which of the following is the correct flow of energy?

A. The crab gets energy from eating plants.

B. The heron gets energy from eating plants.

C. The crab gets energy from eating the heron.

D. The heron gets energy from eating the crab.

3 Kerry drew the following food chain in her science notebook.

plants → grasshoppers → rats → owls
How would the grasshoppers be classified in this food chain?
A. producer B. primary consumer

C. tertiary consumer D. secondary consumer

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4 Very rarely do ecosystems contain more than four trophic levels, because there is not
enough energy to support more. In which trophic level is there the least energy
available?
A. producer

B. primary consumer

C. tertiary consumer

D. secondary consumer
Name Date

SC.912.L.17.11 Biology
Evaluate the costs and benefits of renewable and nonrenewable resources, such as water,
energy, fossil fuels, wildlife, and forests.

STANDARD REVIEW
A natural resource is any natural material that is used by humans. Examples of natural
resources are water, petroleum, minerals, forests, and animals. Most resources are changed
and made into products that make people’s lives more comfortable and convenient. The
energy we get from resources, such as gasoline and wind, ultimately comes from the Sun’s
energy.
Some natural resources can be renewed. A renewable resource is a natural resource that
can be replaced at the same rate at which the resource is used. Although many resources
are renewable, they still can be used up before they can be renewed. Trees, for example,
are renewable. However, some forests are being cut down faster than new forests can grow
to replace them.
Not all of Earth’s natural resources are renewable. A nonrenewable resource is a resource
that forms at a rate that is much slower than the rate at which it is consumed. When these
resources become scarce, humans will have to find other resources to replace them. Most
of the energy we use comes from a group of natural resources called fossil fuels. A fossil
fuel is a nonrenewable energy resource formed from the remains of plants and animals
that lived long ago. Examples of fossil fuels include petroleum, coal, and natural gas. Once
fossil fuels are used up, new supplies won’t be available for thousands—or even millions—
of years. Second, obtaining and using fossil fuels has environmental consequences, such as
acid rain and global warming. To continue to have access to energy and to overcome pol
lution, we must find alternative sources of energy.

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STANDARD PRACTICE
1 When the supplies of a product decrease, the price of the product tends to increase.
How might continuing increases in the price of fossil fuels affect research on the
development of renewable resources, such as wind power and solar energy?
A. Research on the development of renewable resources would likely decline.

B. Research on the development of renewable resources would likely increase.

C. There would be less research on alternative energies and more on fossil fuels.

D. Increased fossil fuel prices would have little effect on research and development.

2 Public officials in Florida had to decide whether to build a new coal-fired electric
power plant or invest in developing more solar energy. Which of the following sums
up the arguments likely made by those who favored coal and those who favored solar
energy?
A. pro coal: coal mines need more business; pro solar: Florida is an ideal state for
solar energy because of its abundant sunshine
B. pro coal: invest in this relatively cheap and reliable energy source; pro solar:
invest in solar energy because coal supplies are running out faster than oil
supplies
C. pro coal: coal is a relatively cheap and abundant energy source; pro solar:
Florida is ideal for solar energy, which produces no pollutants, because of
its abundant sunshine
D. pro coal: a coal-fired power plant gives off less pollution than any other type of
power plant; pro solar: investments in solar energy will help develop technologies
for safer nuclear power plants
3 The picture below shows windmills on a wind farm.

Which of the following is a positive effect of using windmills instead of fossil fuels to
obtain energy?
A. reduction in CO2 emissions
B. decrease in overall electrical use

C. use of more habitat for wind farms

D. increase in the use of metal resources

4 Conservation is the wise use of natural resources. One way to practice conservation is
the three Rs—reduce, reuse, and recycle. When people practice conservation in these
ways, how do they affect the environment?
A. They do not affect the environment.

B. They protect the environment by using fewer natural resources.

C. They decrease the amount of natural resources in the environment.

D. They cause an increase in the amount of pollution in the environment.

Name Date

SC.912.L.17.13 Biology
Discuss the need for adequate monitoring of environmental parameters when making
policy decisions.
SC.912.N.2.1

STANDARD REVIEW
It is easy to get discouraged when considering the world’s many serious environmental
problems. But do not lose track of the conclusion that emerges from our examination
of these environmental problems—each of the world’s many problems is solvable. If one
looks at how environmental problems have been overcome, a clear pattern emerges.
Five Steps to Success
Viewed simply, there are five components to successfully solving any environmental
problem.

1. Assessment. The first stage is scientific analysis of the problem, the gathering
of information about what is happening. To construct a scientific model of an
eco- system, data must be collected and analyzed. A model makes it possible to
describe the current state of the ecosystem. A model would also allow scientists
to make predictions about the future of the ecosystem.
2. Risk analysis. Using the information obtained by scientific analysis, scientists
predict the consequences of different types of environmental intervention. It is
also essential to evaluate any negative effects associated with a plan of action.
3. Public education. When it is possible to describe alternative courses of
action, the public must be informed. This involves explaining the problem in
understandable terms, such as at a public meeting, presenting the alternative
actions available, and explaining the probable costs and results of the different
choices.
4. Political action. The public, through its elected officials, selects and implements
a course of action. Individuals can be influential at this stage by exercising their
right to vote and by contacting their elected officials.
5. Follow-through. The results of any action should be carefully monitored to see
if the environmental problem is being solved.

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STANDARD PRACTICE
1 Imagine that a community plans to build a shopping mall. Which statement describes
how the local government should consider the effects of development on wildlife in
the area?
A. Local governments do not have to consider the impacts of development on
endangered species at all.
B. Local governments always need to consider the needs of the developer first and
the needs of wildlife second.
C. Local governments need to consider if a species is endangered and at risk of
extinction due to habitat destruction.
D. Local governments do not face pressure from environmentalists or developers
when making decisions regarding wildlife.

2 When developing environmental policies, elected officials and environmental activists

often consult scientists to assess problems and verify the results of solutions. Which
actions are part of environmental science as well as environmental activism?
A. taking a side and persuading others to act

B. making assumptions and drawing conclusions

C. observing, collecting data, and controlling variables

D. making educated guesses and implementing good ideas

3 Members of an environmental protection group have noticed that fish populations

have decreased in a local wetland area. They think that pollution in runoff from
nearby farms is to blame. What is the first step they should take to solve the problem
and restore the wetland fish populations?
A. Enact laws that prohibit farming near wetland areas and near creeks that
drain into them.
B. Educate other communities whose wetlands are at risk of the dangers of having
farms nearby.
C. Collect and analyze data to assess which factors are causing the fish
populations to decrease.
D. Bring in fish from other wetlands to restore the original fish population size
of the local wetland area.
4 After implementing a solution to an environmental problem, environmental workers
often continue to collect and analyze data associated with the problem area. Why is it
important for public officials to follow through and check the results of implemented
solutions to environmental problems?
A. Once data is collected in an area, public officials will always have to monitor that
area.
B. The act of collecting data ensures that the problem will never come back again
in that area.
C. Public officials always monitor the environment whether or not there has been
an environmental problem.
D. Collected data can show public officials whether the solution is actually working
or whether it needs to be modified.
Name Date

SC.912.L.17.20 Biology
Predict the impact of individuals on environmental systems and examine how human life-
styles affect sustainability.

STANDARD REVIEW
The global rate of population growth has been declining. The United Nations projects that
the world’s population will stabilize at 9.7 billion by the year 2050. However, population
growth rates are uneven across Earth. Population growth tends to be the highest in coun-
tries that can least afford it. Already limited resources are strained further, and natural
resources—ground water, land for farming, forests—are ever more quickly depleted or
polluted.
No one knows whether the Earth can support six billion people indefinitely, much less the
far larger population that lies in our future. Building a sustainable world is the most im-
portant task facing humanity’s future. The quality of life available to your children in the
new century will depend to a large extent on our success.
One industry where sustainable practices can help support human activity without deplet-
ing natural resources is agriculture. Sustainable agriculture refers to farming that remains
productive and profitable through practices that help replenish the soil’s nutrients, reduce
erosion, and control weeds and insect pests.
In an ecosystem, decomposers return mineral nutrients to the soil. However, when the
plants are harvested and shipped away, there is a net loss of nutrients from the soil where
the plants were growing. The amount of organic matter in the soil also decreases, making
the soil less able to hold water and more likely to erode.
One way to protect soil is through the planting of cover crops. After harvest, farmers can
plant cover crops, such as rye, clover, or vetch, instead of letting the ground lie bare. Cover
crops keep the soil from compacting and washing away, and they help the soil absorb
water. They also provide a habitat for beneficial insects, slow the growth of weeds, and
keep the ground from overheating. When cover crops are plowed under, they return nutri-
ents to the soil.
Rotational grazing can also protect land resources. Farmers who raise cattle and sheep can
divide their pastures into several grazing areas. By rotating their livestock from one area to
another, they can prevent the animals from overgrazing the pasture. This allows the plants
on which the animals feed to live longer and be more productive. Water quality improves
as the pasture vegetation becomes denser. Animals distribute manure more evenly with
rotational grazing than they do in feed lots or unmanaged pastures.

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Name Date

SC.912.L.17.20 Biology
STANDARD PRACTICE
1 Sustainable agriculture is farming that remains productive and profitable while still
conserving natural resources. Which of the following is an example of a sustainable
agriculture practice?
A. preventing cattle from ever grazing on pastures

B. removing all manure from pastures where cattle graze

C. allowing cattle to graze over an entire pasture year round

D. rotating parts of a pasture on which cattle get to graze throughout the year

2 The graph below shows the English sparrow population in an area over several years.

English Sparrow Population

50

40

30
Number of English Sparrows per Square Kilometer

20

10

1972 1976 1980 1984 1988 1992 1996 2000

Year

In 1988, a large shopping mall was built in the area where the sparrows lived.
According to the information in the graph, how did this affect the sparrow
population over a period of time?
A. The population increased because humans fed the sparrows.

B. The sparrow population was unaffected by the shopping mall.

C. The population increased because predators were taken away.

D. The sparrow population decreased because of habitat destruction.

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120 Florida Biology Standards Review
Name Date

SC.912.L.17.20 Biology
3 The world’s population exceeded 6 billion in October 1999, and the annual increase is
now about 94 million people. About 260,000 people are added to the world popula
tion each day, or about 180 every minute. Which of the following is not a result of the
increasing human population?
A. fewer oldgrowth forests

B. more stress on natural resources such as water

C. increased carrying capacity of many ecosystems

D. less undeveloped land available for animal habitats

4 The following graph shows the number of insects present on a field after it has been
sprayed with the same insecticide once a year over four years.

20
18
16
14
12
10
8
6
4
2
0

1 2 3 4
Year

What is the most reasonable hypothesis for the increase in insects seen in year four?
A. The insecticide used in year four was stronger than it was in previous years.

B. The insects in the field in year four were the same species as in previous years.

C. The population of insects resistant to the insecticide had increased over the
four years.
D. Insects from a neighboring field had moved into the area before the insecticide
was applied in year four.

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Name Date

SC.912.L.18.1 Biology
Describe the basic molecular structures and primary functions of the four major categories
of biological macromolecules.

STANDARD REVIEW
Four principal classes of organic compounds are found in living things:
carbohydrates, lipids, proteins, and nucleic acids.
Carbohydrates are organic compounds made of carbon, hydrogen, and oxygen
atoms in the proportion of 1:2:1. Carbohydrates are a key source of energy, and they
are found in most foods. The building blocks of carbohydrates are single sugars,
called monosac-
charides, such as glucose, C6H12O6, and fructose. Disaccharides are double sugars formed
when two monosaccharides are joined. For example, sucrose, or common table sugar,
consists of both glucose and fructose. Polysaccharides such as starch are chains of three
or more monosaccharides. Starch and cellulose, which are found in plants, and glycogen,
which is made by animals, are examples of polysaccharides.
Lipids are nonpolar molecules that are not soluble or mostly insoluble in water. They
include fats, phospholipids, steroids, and waxes. Phospholipids make up the lipid bilayer
of cell membranes. Steroids include cholesterol, which is found in animal cell
membranes. Other lipids include some light-absorbing compounds, such as the plant
pigment chloro- phyll. Fats are lipids that store energy.
Proteins are usually large molecules formed by linked smaller molecules called amino acids.
Amino acids are the building blocks of proteins. Twenty different amino acids are
found in proteins. Some amino acids are polar, and others are nonpolar. Some amino
acids are electrically charged, and others are not charged. Proteins fold into compact
shapes, deter- mined in part by how the protein’s amino acids interact with water and
one another.
Some proteins are enzymes and promote chemical reactions. Other proteins have impor-
tant structural functions. Other proteins called antibodies help your body defend against
infection. Specialized proteins in muscles enable your muscles to contract. In your blood, a
protein called hemoglobin carries oxygen from your lungs to body tissues.
Nucleic acids are long chains of smaller molecules called nucleotides. A nucleotide has
three parts: a sugar, a base, and a phosphate group, which contains phosphorus and oxy-
gen atoms. There are two types of nucleic acids—DNA and RNA—and each type con-
tains four kinds of nucleotides. DNA, or deoxyribonucleic acid, consists of two strands of
nucleotides that spiral around each other. Chromosomes contain long strands of DNA,
which stores hereditary information. RNA, or ribonucleic acid, may consist of a single
strand of nucleotides or of based-paired nucleotides. RNA plays many key roles in the
manufacture of proteins.

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STANDARD PRACTICE
1 Two of the four principle classes of organic compounds are proteins and nucleic
acids. What is the relationship between proteins and nucleic acids?
A. Nucleic acids use proteins for energy.

B. Nucleic acids are a subset of proteins.

C. Proteins are long polymers of nucleic acids.

D. Nucleic acids contain the information to make proteins.

2 Proteins are used to enable movement, provide structure and support, and carry out
important chemical reactions inside the body. What is needed in order for the human
body to synthesize proteins?
A. a diet rich in amino acids

B. sufficient sunlight and water

C. 1,200 calories of nutrients per day

D. minerals and fats in sufficient amounts

3 The diagram below illustrates the structure of glucose.

Which of the following describes how the body uses molecules like the one illustrated
above?
A. Sugars form carbohydrates, which are linked together to form fats that
store energy.
B. Carbohydrates are broken down into glucose, which is stored inside the body’s
cells.
C. Sugars are broken down into proteins that become the building blocks of the
body’s tissues.
D. Carbohydrates, such as glucose, are broken down to release chemical energy
that is used by the body’s cells.
4 Phospholipids are linear molecules with one polar end and one nonpolar end. How
does this structure relate to their function in cell membranes?
A. The polar tails of phospholipids form the outside of the cell membrane.

B. The nonpolar heads of phospholipids form the outside of the cell membrane.

C. The polar heads of phospholipids combine to form the middle of the cell
membrane, with the tails on the outside.
D. The nonpolar tails of phospholipids combine to form the middle of the cell
membrane, with the heads on the outside.
Name Date

SC.912.L.18.7 Biology
Identify the reactants, products, and basic functions of photosynthesis.

STANDARD REVIEW
Photosynthesis is the process that provides energy for almost all life. As the figure below

ADP CO2
O2
H 2O
ATP

Light
Stage 1 Stage 2 Stage 3

NADP+

NADPH
Organic compounds

shows, photosynthesis has three stages:

Stage 1: Energy is captured from sunlight. Pigments such as chlorophyll absorb light
energy, which excites electrons.
Stage 2: Light energy is converted to chemical energy, which is temporarily stored in ATP
and the energy carrier molecule NADPH. This stage occurs when the excited electrons are
passed through a series of molecules—called an electron transport chain—along a
thylakoid membrane.
Stage 3: The chemical energy stored in ATP and NADPH powers the formation of organic
compounds, using carbon dioxide, CO2. The most common way that this happens is called
the Calvin cycle.
Photosynthesis occurs in the chloroplasts of plant cells and algae and in the cell membrane
of certain prokaryotes. Photosynthesis can be summarized by the following equation:
6CO2 1 6H2O → C6H12O6 1 6O2
[carbon dioxide] [water] [light] [sugars] [oxygen gas]

6CO
1 6H O light 1 6O
2 2 CH
6 O
12 6 2
carbon water sugars oxygen
dioxide gas

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STANDARD PRACTICE
1 The illustration below shows a single-cell organism.

Nucleus

Chloroplast

Cell wall

What is the role of the chloroplasts in this organism?

A. to store energy

B. to absorb water

C. to aid in asexual reproduction

D. to make food through the process of photosynthesis

2 Latecia conducted an experimental investigation of the gas production of a water

plant. She placed a beaker upside down over a water plant submerged in water and
collected the gas that the water plant produced when kept in sunlight. After several
days, a large bubble of gas collected in the upside-down beaker.

Given that the gas came from the water plant, what are the contents of the bubble
of gas collected in the test tube?
A. The gas contains one of the products of respiration, oxygen.

B. The gas contains one of the products of photosynthesis, oxygen.

C. The gas contains one of the products of respiration, carbon dioxide.

D. The gas contains one of the products of photosynthesis, carbon dioxide.

3 Daniel found the equation below in a textbook about cells.
6CO2 1 6H2O → C6H12O6 1 6O2
[carbon dioxide] [water] [light] [sugars] [oxygen gas]

6CO
1 6H O light 1 6O
2 2 CH
6 O
12 6 2
carbon water sugars oxygen
dioxide gas
What process is described by this equation?
A. cellular respiration

B. fermentation

C. glycolysis

D. photosynthesis

4 Autotrophs, such as plants, use light to make their own food. What happens to the
light absorbed by a plant during photosynthesis?
A. It is converted to kinetic energy.

B. It is converted to chemical energy, which the plant stores.

C. It powers a reaction that produces carbon dioxide and water.

D. It powers a reaction that produces oxygen and carbon dioxide.

Name Date

SC.912.L.18.8 Biology
Identify the reactants, products, and basic functions of aerobic and anaerobic
cellular respiration.

STANDARD REVIEW
Most of the foods we eat contain usable energy. Much of the energy in a banana, for
example, is stored in proteins, carbohydrates, and fats. But before you can use that
energy, it is transferred to ATP. Like in most organisms, your cells transfer the energy
in organic
compounds, especially glucose, to ATP through a process called cellular respiration.
Oxygen in the air you breathe makes the production of ATP more efficient, although
some ATP is made without oxygen. Metabolic processes that require oxygen are called
aerobic. Meta bolic processes that do not require oxygen are called anaerobic, meaning
“without air.”
Cellular respiration is the process cells use to harvest the energy in organic compounds,
particularly glucose. The breakdown of glucose during cellular respiration can be summa
rized by the following equation:
C6H12O6 1 6O2 → 6CO2 1 6H2O 1 energy
[glucose] [oxygen gas] [enzymes] [carbon dioxide] [water] [ATP]

CHO
1 6O enzymes 6CO 1 6H O 1 energy
6 12 6 2 2 2
glucose oxygen carbon water ATP
gas dioxide

As the figure below shows, cellular respiration occurs in two stages.

Stage 1: Glucose is converted to pyruvate, producing a small amount of ATP and NADH.
Stage 2: When oxygen is present, pyruvate and NADH are used to make a large
amount of ATP. This process is called aerobic respiration. Aerobic respiration occurs
in the mitochon dria of eukaryotic cells and in the cell membrane of prokaryotic cells.
When oxygen is not present, pyruvate is converted to either lactate or ethanol and
carbon dioxide.
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STANDARD PRACTICE
1 The flow chart below shows a process that generates ATP.
Pyruvate 1 O2 → Krebs cycle → Electron transport
chain Where does this process take place within a cell?
A. chloroplast B. mitochondria

C. nucleus D. ribosome

2 Cells use sugars to produce energy through several different processes. Which process
would provide the most energy for cell functions?
A. glycolysis

B. aerobic respiration

C. aerobic fermentation

D. anaerobic fermentation

3 The Krebs cycle, fermentation, electron transport, and the breakdown of starch are
processes of respiration. Which process could take place within an airtight bottle
that contained no oxygen?
A. Krebs cycle

B. fermentation

C. electron transport

D. breakdown of starch

4 Running for a long period of time, such as during a marathon, can result in
muscle fatigue. Which process is important to a runner at the end of a
marathon?
A. the electron transport chain to produce ATP

B. the Krebs cycle to produce NADH and FADH2

C. alcoholic fermentation to release carbon dioxide

D. lactic acid fermentation to produce ATP without oxygen

Name Date

SC.912.L.18.8 Biology
5 There are two kinds of fermentation. What results from alcoholic fermentation?
A. the production of lactate

B. the production of oxygen

C. the production of ethanol

D. the production of pyruvate

6 Humans and other heterotrophs get their energy from eating food. What process
in human body cells transfers the energy stored in a grilled cheese sandwich to
ATP?
A. electrolysis

B. Calvin cycle

C. photosynthesis

D. cellular respiration

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130 Florida Biology Standards Review
Name Date

SC.912.L.18.9 Biology
Explain the interrelated nature of photosynthesis and cellular respiration.
SC.912.E.7.1

STANDARD REVIEW
Photosynthesis and cellular respiration are related processes. The diagram below shows
how the substances produced by photosynthesis are used during cellular respiration and
vice versa.

ATP produced in cellular respiration provides energy for life processes.

Solar energy s converted to chemical energy during

photosynthesis.
Light energy ATP

Chloroplast Mitochondrion

Chloroplasts contain pigments that absorb light to provide energy for photosynthesis. Cellular respira- tion takes place n mitochondria

Plant cell Plant cell Animal cell

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STANDARD PRACTICE
1 Plant cells have mitochondria, which use glucose to produce ATP. Where does the
glucose originate?
A. photosynthesis, which occurs in chloroplasts

B. fermentation, which occurs in the absence of oxygen

C. exocytosis, which transports the glucose into the cells

D. diffusion, which transports the glucose from the digestive tract

2 Photosynthesis and cellular respiration are interrelated processes. During which

biogeochemical cycle do the biological processes of photosynthesis and cellular
respiration play key roles?
A. carbon cycle B. nitrogen cycle

C. hydrogen cycle D. phosphorus cycle

3 A carbon sink is a part of the Earth’s ecosystem that stores carbon in one form or
another for hundreds, thousands, and even millions of years. Forests and oceans
are known to be carbon sinks. Which of the following marine organisms play a
major role in making the ocean a carbon sink?
A. marine mammals, because they are high up on the food chain

B. whales, because they are large and can take up large amounts of carbon dioxide

C. seaweeds and phytoplankton, because they use carbon dioxide during

photosynthesis
D. zooplankton, because they make shells and skeletons from calcium carbonate
dissolved in seawater

4 The general equations for photosynthesis and cellular respiration show how the two
processes are interconnected in natural cycles. Which substance is a product of
cellular respiration and a reactant in photosynthesis?
A. ATP B. glucose

C. oxygen D. water
Name Date

SC.912.L.18.10 Biology
Connect the role of adenosine triphosphate (ATP) to energy transfers within a cell.

STANDARD REVIEW
ATP, or adenosine triphosphate, is an important biological molecule. ATP is composed of
a single nucleotide with two extra energy-storing phosphate groups. When food molecules
are broken down inside cells, some of the energy in the molecules is stored temporarily in
ATP. Cells need a steady supply of ATP to function.
When a log burns, the energy stored in wood is released quickly as heat and light. But in
cells, chemical energy stored in food molecules is released gradually in a series of
enzyme- assisted chemical reactions. The product of one chemical reaction becomes a
reactant in the next reaction. In the breakdown of starch, for example, each reaction
releases energy.
When cells break down food molecules, some of the energy in the molecules is released
as heat. Much of the remaining energy is stored temporarily in molecules of ATP. Like
money, ATP is a portable form of energy “currency” inside cells. ATP delivers energy
wherever energy is needed in a cell. The energy released from ATP can be used to power
other chemical reactions, such as those that build molecules. In cells, most chemical reac-
tions require less energy than is released from ATP. Therefore, enough energy is released
from ATP to drive most of a cell’s activities.
The three phosphate groups in ATP form a chain that branches from a five-carbon sugar
called ribose. This phosphate “tail” is unstable because the phosphate groups are nega-
tively charged and therefore repel each other. The phosphate groups store energy like a
compressed spring does. This energy is released when the bonds that hold the phosphate
groups together are broken.
Breaking the outer phosphate bond requires an input of energy. Much more energy is
released, however, than is consumed by the reaction. As shown in the figure below, the
removal of a phosphate group from ATP produces adenosine diphosphate, or ADP.
This reaction releases energy in a way that enables cells to use the energy. Cells use
the energy to power metabolism. The following equation summarizes the reaction:
H2O 1 ATP → ADP 1 P 1 energy
adenosine triphosphate
PP
tri =3

adenosine phosphate
PP
di =2

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STANDARD PRACTICE
1 ATP is a cell’s main form of energy “currency.” Which of the following comprises an
ATP molecule?
A. adenine, the sugar ribose, and three phosphate groups

B. adenine, the sugar deoxyribose, and two phosphate groups

C. a nitrogenous base, a phosphate group, and the sugar ribose

D. a complex molecule of amino acids surrounding Z

2 ATP and ADP both play a role in cellular reactions involving energy. How are these
two molecules related?
A. Adding a phosphate group to ATP produces ADP and releases energy.

B. Adding a phosphate group to ADP produces ATP and releases energy.

C. ADP breaks down to produce ATP and a phosphate group, releasing energy.

D. ATP breaks down to produce ADP and a phosphate group, releasing energy.

3 Daphnia is a small water invertebrate that is almost transparent. Like all animals,
Daphnia require chemical energy to live. It uses structures called gills to extract
oxygen from the water to help it obtain chemical energy. Which statement below
describes how they obtain chemical energy?
A. Daphnia absorb glucose from algae and then use fermentation to release chemi-
cal energy in the form of ATP.
B. Daphnia absorb pyruvate from algae and then use gluconeogenesis to release
chemical energy in the form of ADP.
C. Daphnia absorb glucose from algae and then use cellular respiration to release
chemical energy in the form of ATP.
D. Daphnia absorb glucose from algae and then use cellular respiration to release
chemical energy in the form of NADP.
4 ATP provides the energy needed to carry out many cell functions. Which of the
following processes does not require ATP?
A. making more ATP

B. muscle contraction

C. active transport of protein across a membrane

D. passive transport of oxygen across a cell membrane

Name Date

SC.912.L.18.11 Biology
Explain the role of enzymes as catalysts that lower the activation energy of
biochemical reactions. Identify factors, such as pH and temperature, and their effect on
enzyme activity.

STANDARD REVIEW
The chemical reactions in cells occur quickly and at relatively low temperatures because
of the action of many enzymes. Enzymes are substances that increase the speed of chemi-
cal reactions. Most enzymes are proteins. Enzymes are catalysts, which are substances that
reduce the activation energy of a chemical reaction. An enzyme increases the speed of a
chemical reaction by reducing the activation energy of the reaction. Enzymes help organ-
isms maintain homeostasis. Without enzymes, chemical reactions would not occur quickly
enough to sustain life.
Enzymes assist biochemical reactions by bringing key molecules together. A substance on
which an enzyme acts during a chemical reaction is called a substrate. Enzymes act only
on specific substrates. An enzyme’s shape determines its activity. Typically, an enzyme is a
large protein with one or more deep folds on its surface. These folds form pockets called
active sites. As shown in the figure below, an enzyme’s substrate fits into the active site.
An enzyme acts only on a specific substrate because only that substrate fits into its active
site. Your body’s cells contain many different enzymes, and each enzyme catalyzes a
different chemical reaction.
Substrates Product

Active site
Enzyme

Any factor that changes the shape of an enzyme can affect the enzyme’s activity.
For example, enzymes operate most efficiently within a certain range of
temperatures.
Temperatures outside this range can either break or strengthen some of the enzyme’s
bonds, changing its shape. Moreover, each enzyme operates best within a certain range of
pH values. A pH value outside this range can cause bonds in an enzyme to break, reducing
the enzyme’s effectiveness.

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STANDARD PRACTICE
1 Most enzymes operate most efficiently within a certain range of
temperatures. Enzymes in your body probably work best at what
temperature?
A. 25°C

B. 30°C

C. 37°C

D. 98°C

2 Enzymes are protein catalysts. What is the role of a catalyst?

A. provides extra energy for a reaction

B. lowers the activation energy of a reaction

C. allows only irreversible reactions to happen

D. eliminates the activation energy of a reaction

3 Living things use enzymes in the chemical reactions of metabolism. When food is
digested, it is metabolized to release energy. Which statement best describes the role of
an enzyme in this process?
A. Because enzymes are proteins, they only react with other protein molecules,
resulting in the production of glucose.
B. The active site of the enzyme attached to the substrate of a food molecule
produces carbon molecules, the building blocks of cells.
C. The active site of an enzyme attached to a substrate prevents the chemical
reactions involved in metabolism from happening too quickly.
D. The active site of the enzyme binds to a substrate on a food molecule and
the enzyme changes shape slightly, causing a chemical reaction to happen.
4 The table below lists some digestive enzymes found in the human small intestine.

Digestive Enzymes in the Small Intestine

Enzyme Substrate Digested product
Amylase (pancreas) starch disaccharides
Trypsin (pancreas) proteins peptides
Lipase (pancreas) fat fatty acid and glycerol
Maltase, sucrase, lactase disaccharides monosaccharides
Peptidase peptides amino acids

Based on the information in the table, what would be one effect of an

injured pancreas?
A. The blood levels of monosaccharides would increase.

B. The absorption of amino acids would be more efficient.

C. The body would be unable to process starches, proteins, and fats.

D. The levels of the enzymes amylase, trypsin, and lipase would increase.

5 Many of the proteins in the human body are enzymes that catalyze chemical
reactions. What is the relationship between enzymes and activation energy?
A. When an enzyme catalyzes a reaction, it increases the activation energy of the
reaction.
B. When an enzyme catalyzes a reaction, it increases the activation energy of
the product.
C. When an enzyme catalyzes a reaction, it decreases the activation energy of the
reaction.
D. When an enzyme catalyzes a reaction, it does not affect the activation
energy of the reaction.
Name Date

SC.912.L.18.12 Biology
Discuss the special properties of water that contribute to Earth’s suitability as an
environ- ment for life: cohesive behavior, ability to moderate temperature, expansion
upon freezing, and versatility as a solvent.

STANDARD REVIEW
Water has many unique properties that make it an important substance for life. One
such property is its ability to store heat energy. Water absorbs heat more slowly and
retains this energy longer than many other substances do. Many organisms release
excess heat through water evaporation. For example, humans cool themselves by
sweating. The water vapor lost through the evaporation of sweat carries heat away from
the body.
Two other important properties of water are cohesion and adhesion. Cohesion is an attrac-
tion between substances of the same kind. The hydrogen bonds between water molecules
cause the cohesion of liquid water. Because of cohesion, water and other liquids form thin
films and drops. Adhesion is an attraction between different substances. Water molecules
are attracted to many other similarly polar substances. Adhesion powers a process, called
capillary action, in which water molecules move upward through a narrow tube, such as
the stem of a plant.
Another important property of water is its ability to dissolve many substances. A solution
is a mixture in which one or more substances are evenly distributed in another substance.
The polarity of water enables many substances to dissolve in water. Ionic compounds and
polar molecules dissolve best in water. Nonpolar molecules, however, do not dissolve well
in water.

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Name Date

SC.912.L.18.12 Biology
STANDARD PRACTICE
1 A florist places a bouquet of white carnations in water containing blue dye. After a
time, the flowers turn blue. What process helped the carnations to change color?
A. water’s ability to form crystals

B. ability of H2O to dissolve NaCl

C. cohesion and adhesion of water molecules

D. formation of covalent bonds between hydrogen and oxygen molecules

2 Which of these statements correctly explains why water is a good solvent for many
ionic compounds?
A. Water is a polar compound.

B. Water is an ionic compound.

C. Water molecules are nonpolar.

D. Water molecules completely separate into ions in solution.

3 What is the attractive force between two of the same kind of particle?
A. adhesion

B. capillary action

C. cohesion

D. polarity

4 Which of the following is not a unique property of water?

A. Water molecules stick together by cohesion.

B. Frozen water is less dense than liquid water.

C. Water molecules stick to other polar substances.

D. Hydrogen bonds allow water to heat and cool very quickly.

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Name Date

SC.912.L.18.12 Biology
5 Water has the ability to store heat longer than other substances. What benefit does
this property of water provide to organisms?
A. It provides the ability to dissolve nonpolar compounds.

B. It provides the appropriate catalyst for chemical reactions.

C. It enables organisms to function with a higher cellular pH.

D. It allows organisms to maintain a stable internal temperature.

6 Many fish and aquatic plants can survive a cold winter because the layer of ice
that forms at the top of a lake insulates the water below and prevents the lake
from freezing solid. What unique property of water contributes to this effect?
A. Water absorbs heat when it evaporates and forms a gas.

B. Water expands and becomes less dense when it freezes.

C. Water molecules completely separate into ions in solution.

D. Water forms hydrogen bonds with ions and other polar substances.

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Name Date

PRACTICE TEST 1
1 A scientist notices that a local population of frogs has started to develop deformities.
He designs an experiment to identify the source of the deformities, using unhatched
frog eggs and a variety of environmental stimuli. Which of the following would be the
dependent variable in this experiment?
A. the introduction of pesticides to the frog eggs

B. the number of frog eggs used in the study’s sample

C. the development of deformities in the hatched frogs

D. the size of the aquariums in which the frog eggs were kept

2 A scientist wants to draw conclusions about all mammals based on the results of a
study in which she compares the anatomies of house cats, leopards, seals, goats, and
frogs. Her peers suggest that data from other studies might better support her
conclusions. Why might this experiment be a poor choice for finding data that
support her conclusions?
A. The study does not include mammals, so she cannot use the data to draw conclu-
sions about mammals.
B. The study includes only mammals, so she cannot use the data to make conclu-
sions about invertebrates.
C. The study includes only vertebrates, so she cannot use the data to draw conclu-
sions about invertebrate mammals.
D. The study includes only a few mammals, so she cannot use the data to safely
draw conclusions about all mammals.

3 Most scientists have biases, but they try to prevent bias from influencing their work.
What is bias?
A. having a point of view that is objective

B. having a point of view that is impartial

C. having a point of view that lacks prejudice

D. having a point of view that can influence an experiment

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Name Date

4 Water movement into and out of cells is of prime importance to all living things. A
single-celled organism has star-shaped organelles called contractile vacuoles to move
water from inside to outside the cell. The data presented in the table below were
obtained in an experiment in which the organism was placed in water with different
salt concentrations.
Salt concentration Rate of contractile vacuole
contractions per minute
Very high 2
High 8
Medium 15
Low 22
Very low 30
The rate at which the contractile vacuole contracted to pump out excess water was
recorded. How could you explain the observed relationship between the rate of
contractile vacuole concentration and the salt concentration?
A. When the salt concentration outside the cell is very high, diffusion causes
water to move inside the cell, and the contractile vacuole has to contract
more rapidly.
B. When the salt concentration outside the cell is very low, diffusion causes
water to move outside the cell, and the contractile vacuole has to contract
more rapidly.
C. When the salt concentration outside the cell is very high, diffusion causes
water to move outside the cell, and the contractile vacuole does not need to
contract as rapidly.
D. When the salt concentration outside the cell is very low, diffusion causes
water to move outside the cell, and the contractile vacuole does not need to
contract as rapidly.

5 The diagram below shows a newspaper headline.

What is this headline an example of?

A. a fact B. a prediction

C. a hypothesis D. an observation
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6 The table below contains data that 7
A scientific theory is not just good
are part of a scientific investigation. guesswork. There are several steps
A-, -5384 A9,5(-, .,/-.7 /2 *,27/1,7,56 involved in developing a theory,
/2 :,(56 F$+ *$ M *$
A1 !(/1' 50 51
and these steps could take many
2
4
87
103
88
104
years before a hypothesis becomes
6 117 118 accepted as a scientific theory.
8 128 128
10
12
139
152
139
149
Step 1
14 160 162 Question
16 163 172
18 163 174

Step 2
Which of the following is not a Form
testable hypothesis that this data testable
could be used to support or hypothesis
disprove? Step 3
Hypothesis supported by experiment
A. Height is affected by child-
hood nutrition.
Step 4
B. All females are taller than
?
males at age 12.
C. Being taller is always Theory
better than being shorter.
In the diagram, what process
D. All men who are married are happens at Step 4?
taller than their wives.
A. A team of scientists must form
a committee to discuss the
hypothesis.
B. The results of the experiment
must be published along with
a ballot so that other scientists
can vote on whether they sup-
port or reject the hypothesis.
C. The scientist or team of
scien- tists who formulated
the hypothesis must do the
sup- porting experiment over
and over again to be sure
they have it right.
D. The results of the experiment
must be published so that the
experiment can be repeated by
other scientists, along with
additional experiments to prove
or disprove the hypothesis.
8 The cumulative work of many scientists can often be summarized as theories or laws.
How is a theory different from a law?
A. A theory is a preliminary scientific explanation that can become a law only
when it gains enough support and matches many observed phenomena.
B. A law is a preliminary scientific explanation that can become a theory only
when it gains enough support and matches many observed phenomena.
C. A theory is a well-supported scientific explanation that makes useful predictions
about phenomena, while a law is a well-supported description of observed
phenomena.
D. A theory is an educated guess that can be tested through scientific observation
and experimentation, while a law is a well-supported scientific explanation that
makes useful predictions about phenomena.

9 The cell theory states that all cells arise from existing cells. Which scientist
first determined this part of the cell theory?
A. Hooke

B. Schleiden

C. Schwann

D. Virchow

10 Many organelles in a cell are bound by membranes. Which organelles consist of

membranous tubes and sacs and serve as part of the cell’s packaging and transport
system?
A. mitochondria and lysosomes

B. mitochondria and chloroplasts

C. ribosomes and endoplasmic reticulum

D. Golgi apparatus and endoplasmic reticulum

11 The diagram below shows a cell.
Vesicle Chloroplast Golgi apparatus

Cell membrane
Smooth ER

Cell wall Nucleolus

Central vacuole Nucleus Cytoskeleton
fibers

Mitochondrion
Rough ER

Ribosome

What type of organism might contain this type of cell?

A. animal

B. bacterium

C. plant

D. prokaryote

12 A scientist wants to compare the three-dimensional shape of the chloroplasts in a

plant’s leaf cells and stem cells. Which of the following instruments would be best for
the scientist to use?
A. a computer’s digital camera

B. a compound light microscope

C. a scanning electron microscope

D. a transmission electron microscope

13 European sailors during the age of exploration in the 1500s and 1600s often spent
long months at sea with little to eat other than hard biscuits and dried meat. They
often developed a disease called scurvy, characterized by bleeding gums and loose
teeth. On some ships, the disease was prevented by having sailors eat citrus fruits such
as lemons and limes. How did the citrus fruits prevent scurvy?
A. The high water content reduced dehydration.
B. They provided sailors with sufficient vitamin C.

C. They improved digestion by increasing fiber levels.

D. The calcium content made up for the lack of dairy products.

14 The illustration below shows a plant’s root system.
Phloem

Xylem

Root cap

Growth region

Based on the illustration, which of the following statements is true?

A. Roots grow from their tips.

B. Xylem forms a protective layer over the root cap.

C. Roots contain xylem tissue but not phloem tissue.

D. Most plants have only one growth region in their root system.
15 The diagram below shows the major parts of the human brain.

A
C

B
D

Which part of the brain receives sensory information from all parts of the body
and relays the information to appropriate areas of the cerebral cortex?
A. part A B. part B

C. part D D. part E

16 The picture below shows the exchange of oxygen and carbon dioxide through a
capillary wall.

O2
cO 2
BLOOD
Alveolus

capillary wall

This picture shows the exchange of gases between which two body systems?
A. circulatory and digestive

B. circulatory and respiratory

C. endocrine and circulatory

D. respiratory and endocrine

17 The inflammatory response is one of the immune system’s nonspecific responses to
infection. How does the inflammatory response act as a defense against infection?
A. The high body temperature kills the disease-causing bacteria.

B. Oil and sweat on the surface of the skin inhibit bacterial growth.

C. Body heat destroys cellular proteins needed by the invaders to reproduce.

D. Release of histamine increases blood flow, which brings white blood cells.

18 While cutting through the side of a mountain to build a highway, workers expose
several layers of rock, as shown in the figure below. Fossils of whale bones,
shark teeth, and sand dollars are found in the rocks that form Layer 4. Fossils of
rodents, toads, birds, and lizards are found in Layer 2.

Layer 1
Layer 2
Layer 3
Layer 4

Layer 5

ROCK LAYERS

The observations above support which of the following conclusions?

A. When Layer 4 formed, the mountain was near a large lake.

B. When Layer 4 formed, whales, sharks, and sand dollars lived in the mountains.

C. When Layer 4 formed, an ocean covered the area where the fossils were found.

D. When Layer 4 formed, whales, sharks, lizards, toads, and rodents were in the
same food web.
 Name Date

In a classroom experiment,
19 high school students conducted a survey to determine the similarities and
differences among groups of invertebrates. They recorded their observations in Figure 1. They also made a
chart of phyla in the animal kingdom showing evolutionary milestones (Figure 2). They wanted to know
where each invertebrate specimen they examined would fit on a phylogenetic tree, with Chordata being the
most evolved and Porifera being the least evolved.
Examine the phyla in the chart of evolutionary milestones. Which two of the animals studied would be most
!NIMAL 0HYLUM 3YMMETRY )NTERNAL BODY /THER
PLAN OBSERVATIONS
3PONGE 0ORIFERA !SYMMETRICAL &ULL OF HOLES 0HYLUM %VOLUTIONARY MILESTONE
(YDRA #NIDARIA 2ADIAL 4ISSUES #HORDATA .OTOCORD
3TINGING CELLS %CHINODERMATA $EUTEROSOMES
0LANARIAN 0LATYHELMINTHES "ILATERAL !COELOMATE 'UT HAS ONE !RTHROPODA *OINTED APPENDAGES
OPENING
!NNELIDA 3EGMENTATION
2OUNDWORM .EMATODA "ILATERAL 0SEUDOCOELOMATE 4HREADLIKE
-OLLUSCA #OELOM
%ARTHWORM !NNELIDA "ILATERAL #OELOMATE 3EGMENTED
.EMATODA 0SEUDOCOELOM
3NAIL -OLLUSCA "ILATERAL #OELOMATE -USCULAR FOOT
0LATYHELMINTHES "ILATERAL SYMMETRY
"EETLE !RTHROPODA "ILATERAL #OELOMATE 0AIRED LEGS
#NIDARIA 4ISSUES
3TAR FISH %CHINODERMATA "ILATERAL #OELOMATE &IVE ARMS WITH
TUBE FEET 0ORIFERA -ULTICELLULARITY

closely related, according to the evolutionary hypothesis represented by a phylogenetic tree?

A. the earthworm and the planarian

B. the earthworm and the beetle

C. the sponge and the snail

D. the starfish and the hydra

20 Early biologists thought that sponges were plants. What evidence supports this
classification?
A. Sponges have specialized cells.

B. Sponges prey on small animals.

C. Sponges carry out photosynthesis.

D. Sponges move very slowly if they move at all.

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21 The single-celled organism shown below uses a whip-like flagellum to move. The
organism has a nucleus and organelles surrounded by a membrane. The organism also
has chloroplasts and a cell wall.

Nucleus

Chloroplast

Cell wall

To which kingdom does the organism belong?

A. Animalia B. Bacteria

C. Eubacteria D. Protista

22 Miller and Urey subjected hydrogen gas, water vapor, ammonia, and methane gases
to sparks in a reacting chamber. What was produced, giving support to the
primordial soup model of the origin of life?
A. primitive plants B. organic compounds

C. single-celled organisms D. membrane-bound organelles

23 In what genus are humans classified?

A. sapiens B. Homo

C. Canis D. Animalia

24 Banyan trees are found in places such as Florida, Hawaii, and India. The unique
characteristic of this tree is that roots grow down from its branches into the ground.
The tree can appear to have several trunks. What advantage does the banyan tree have
over other trees?
A. The roots make the tree more stable in high winds.

B. Above-ground roots are more protected from the Sun.

C. The roots provide shelter for ground-dwelling animals.

D. Above-ground roots may dry out and die during droughts.

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25 A biology student measured the height of every student in her class and developed
the histogram shown below. She then used a statistics software program to fit a curve
through her data.

Height Distribution
10
Histogram

Number of individuals
Distribution curve
8

4
< 11.0–1.1 1.2–1.3 1.4–1.5 1.6–1.7 1.8–1.9 > 2
2 Height (m)

0
A group of exchange students visited the school for a semester. The average height of
these students was 1.7 m. This changed the histogram of height in the class. What
type of force on the class gene pool does this event represent?
A. genetic drift

B. immigration

C. mutation

D. natural selection

26 Genetic variation can increase the pace of evolution. Which would most likely
increase genetic variation in a population?
A. cloning

B. twinning

C. crossing-over

D. asexual reproduction

27 In humans, having freckles (F) is dominant to not having freckles (f). Having a cleft
chin (C) is also dominant to not having a cleft chin (c). Which statement is true of the
offspring of a cross between parents that are both heterozygous for both traits (FfCc)?
A. Any offspring with freckles must also have a cleft chin.
B. All of the offspring will be heterozygous for both traits.

C. Any offspring who does not have freckles must have a cleft chin.

D. The offspring could exhibit both traits, neither trait, or only one of the traits.

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28 The Punnett square below shows a cross between two rabbits. Black fur
(B) is dominant to brown fur (b).

B b

B 1 2
Bb � Bb
b 3 4

If individuals from box 1 and box 4 were crossed, what would be the genotypes of the
offspring?
A. all Bb B. Bb and bb

C. BB and bb D. BB and Bb

29 The illustration below shows a cellular process.

Which of the following is shown in this illustration?

A. transcription

B. gene expression

C. DNA translation

D. DNA replication
30 The sequence of bases in a nucleotide of DNA makes up an organism’s genetic code.
How could a change in a sequence of DNA bases affect an organism?
A. DNA could change into RNA.

B. The function of the resulting protein could change.

C. The DNA nucleotide could form an ATP nucleotide.

D. The gene could code for carbohydrates instead of proteins.

31 The diagram below shows the processes that occur during gene expression.

B C D

RNA
DNA

Which of the following is represented at letter B?

A. translation B. replication

C. transcription D. protein synthesis

32 Proteins control the cell cycle in healthy eukaryotes, causing cells to divide at a
healthy rate. What disease is caused by uncontrolled, abnormal cell division?
A. cancer B. heart disease

C. leprosy D. tuberculosis

33 According to the genetic code, the mRNA codons UCU, UCC, UCA, and UCG all
code for the amino acid serine. What does this fact tell you?
A. The genetic code is the same for nearly all organisms.

B. The genetic code does not dictate the amino acid sequence of proteins.

C. A mutation in one base will always have a physical effect on the resulting
protein.
D. A mutation in one base could have absolutely no physical effect on the resulting
protein.
34 The diagram below illustrates the concept of gene flow.

'ENE &LOW

#ROP 1 #ROP 2

Which could be caused by gene flow from a crop plant engineered to be resistant to
herbicides?
A. Drug-resistant bacteria may evolve.
B. New species of poisonous insects may evolve.
C. The genetically engineered plants may eventually loose their herbicide resistance
as it wears off.
D. Genetically engineered plants may cause the evolution of weeds that are immune
to weed-killing chemicals.

35 The diagram shows a sperm cell.

Which statement is true?

A. The sperm’s nucleus is found in part A.
B. The sperm’s nucleus is found in part B.
C. The sperm’s nucleus is found in part C.
D. A sperm cell has no nucleus.
Name Date

36 Octavio drew the diagram below to In asexual reproduction, a

illustrate a process in cells. 38 single parent passes copies of
all of its genes to each of its
offspring.
When is asexual reproduction in
plants advantageous?
A. when the environment is
unstable
B. when there is little competi-
tion for resources
C. when plants are well adapted
to the environment
D. when there are multiple dis-
ease agents in the environment

polar bodies.
What process is shown?
A. fertilization

B. meiosis

C. mitosis

D. osmosis

37 Meiosis is the form of cell

division that produces gametes.
Which of the following
statements correctly describes
gametes?
A. In spermatogenesis, eight
sperm cells are produced.
B. Eggs are diploid and, when
fertilized, give rise to haploid
cells.
C. In the formation of eggs,
four identical haploid cells
are produced.
D. In oogenesis, the cytoplasm
divides unequally, producing
an ovum and three smaller
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 Name Date

The populations of fish

and amphibians in a
lake more than 100
miles downwind from a
coal- fired power plant 39
suddenly began to
decline. Biologists
sampled the water in
the lake and found that
it had a pH of 2.4.
What conclusions could
the biologists draw
from their finding?
A. Acid rain that
had fallen on
the lake had to
have come from
a pollution
source close to
the lake.
B. Pollutants
from the
power plant
could be
making the
lake water
too alkaline
to support
life.
C. The pH of the
lake water was
normal, so the
decline in fish
and amphibian
populations had
some other
cause.
D. The lake water
had become
very acidic,
which could be
linked to
emissions from
the distant
power plant.

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40 Even a tiny amount of moisture can support the life of mosses, which are nonvascular
plants. Why are mosses good pioneer species?
A. They grow very slowly.

B. They can survive in wet areas.

C. They can create a layer of soil on bare rock over time.

D. They can survive in areas that receive low levels of sunlight.

41 All populations fluctuate in size, influenced by both abiotic and biotic factors.
Which of the following is a biotic factor that would most likely increase the size of
a population?
A. increased sunlight

B. reduced competition

C. emergence of a new disease

D. decreased availability of a food source

42 Two species of finches are in competition for the limited resources of an ecosystem.
One species eats fruit and the second species eats seeds. If a third species of herbivore
finches moves into the area, how will the ecosystem change?
A. Resources will be more abundant.

B. Water will become more available.

C. The climate of the ecosystem will change.

D. Fruit and seed resources will become less abundant.
43 The diagram below is an energy pyramid.

%!',%

315)22%,3

6%'%4!4)/.

Which of the following is the correct flow of energy?

A. The eagle gets energy from eating plants.

B. The vegetation gets energy from the squirrels.

C. The squirrels get energy from eating the eagle.

D. The eagle gets energy from eating the squirrels.

44 A nonrenewable resource is a resource that forms at a rate that is much slower than
the rate at which it is consumed. Which of the following resources is a nonrenewable
resource?
A. coal

B. trees

C. wildlife

D. fresh water

45 Before implementing any one solution to an environmental problem, scientists first

analyze collected data to predict the consequences of the different possible solutions.
Why is it important to perform such a risk analysis before taking action?
A. It can prevent the environmental problem from ever occurring in any other unre-
lated area.
B. It can convince the public that there is no need to waste money on follow-up
investigations.
C. It can help officials avoid or prepare for any negative effects associated with a
plan of action.
D. It can solve the environmental problem without the need of public education or
political action.
46 Carbon dioxide is released when 48 One contribution to the buildup of
people burn fuel. It is also used by greenhouse gases in the atmosphere
plants during photosynthesis. Why is from humans’ use of fossil fuels.
is carbon dioxide then considered Fossil fuels result from the gradual
an air pollutant? transformation of layers of organic
A. Too much carbon dioxide matter in sediment into natural gas,
can lead to illness in coal, and petroleum. When fossil
humans. fuels are burned, they release a
B. Carbon dioxide can cause greenhouse gas that can be used by
undesirable changes in climate. plants for photosynthesis. Thus,
planting more trees can partly
C. Carbon dioxide is responsible reduce the effect of burning fossil
for changes in the ozone layer. fuels. What greenhouse gas is
D. Carbon dioxide can mix released when fossil fuels are
with other gases to cause air burned and also plays a major role
pollution. in photosynthesis?
A. carbon dioxide

B. carbon monoxide
47 Four categories of complex, highly C. methane
organized molecular substances are
needed for nearly all of the D. oxygen
processes of organisms. Which
category contains the substances
produced in the body to control the
rate of chemical reactions? 49 Hans Krebs, a German biochemist,
was awarded a Nobel Prize in 1953
A. carbohydrates for the discovery of an important
B. lipids cycle. What does his cycle
represent?
C. nucleic acids
A. the last stage of
D. proteins
photosynthesis
B. the first stage of aerobic
respiration
C. the process of carbon dioxide
fixation
D. the flow of hydrogen ions
through the mitochondria
Name Date

50 The chemical equations that sum up photosynthesis and cellular respiration have
many of the same substances because these two processes are interrelated. Which two
substances are the products of one of these processes and the reactants of the other
process?
A. oxygen and water

B. glucose and water

C. carbon dioxide and water

D. carbon dioxide and glucose

51 Living things get the energy they need from carbohydrates such as glucose. What is
the relationship between carbohydrates and ATP?
A. ATP is a type of carbohydrate.

B. ATP produces carbohydrates in the cell.

C. Cells use carbohydrates to produce ATP.

D. ATP and carbohydrates react to form proteins.

52 The diagram below shows how living things use enzymes in chemical reactions to
release energy.
Substrates Product

Active site
Enzyme

How do enzymes affect the reactions in which they take part?

A. Most enzymes slow down chemical reactions.

B. Enzymes are converted into products in the reaction.

C. Enzymes increase the activation energy of the reaction.

D. Enzymes decrease the activation energy of the reaction.

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53 Water has both cohesive and 55 There is a pair of identical twins in

adhesive forces that are relatively Tanisha’s class. One of them was
strong. That is, its molecules are affected by a severe illness during
strongly attracted to one another his first few months of life.
and to many other kinds of Although he survived, he is much
molecules. What kind of bond shorter than his twin brother. How
forms between two different water is this difference explained?
molecules?
A. The genetic code of any indi-
A. compound bond vidual uniquely determines all
B. double bond polygenic traits.

C. hydrogen bond B. Height is a trait that can be

influenced by both environ-
D. ionic bond mental and genetic factors.
C. The disease caused a mutation
in the smaller twin’s DNA,
resulting in a smaller pheno-
54 Javier notices that one of his house
type.
plants has drooping leaves. Which
description explains what happens D. The twins’ genotype is identi-
when he waters the plant? cal, so the smaller twin will
have to end up the same size
A. The sudden movement of as his brother.
water by osmosis into the
plant cells causes the cells to
swell and burst.
B. The environment changes 56 Which of the following would most
from hypotonic to hypertonic, likely remove carbon dioxide from
the central vacuole swells, and the air?
the leaves stop drooping.
A. Burning logs.
C. The environment changes
from isotonic to hypertonic, B. Planting trees.
mitochondria in the plant C. Killing weeds.
cells take up the additional
water, and the leaves stop D. Breeding livestock.
drooping.
D. The environment changes
from isotonic to hypotonic,
water moves into cells by
osmosis, vesicles in the plant
cells swell, and the leaves stop
drooping.

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57 Male birds of paradise have 59 The development of recombinant
extremely long, showy tail feathers. DNA technology was a major step
Which of the following statements forward in genetic science. Which
best describes a selective advantage of the following developments
for having this trait? represents an advance in medicine
A. The bird is easier for that the application of genetic
predators to spot. science allowed?
B. The bird must eat more to A. development of genetic coun-
produce extra-large seling as a career
feathers.
B. development of a method
C. The bird flies slowly of DNA fingerprinting
because of drag created by
C. production of proteins, such
the feathers.
as insulin, for use as drugs
D. The bird attracts more females
D. cloning of animals and engi-
and therefore mates more
neering of agricultural crops
frequently.

If a heterozygous individual (Aa)

58 60 In 1998, forest fires swept through
were crossed with another
the forests of Yellowstone National
heterozygous individual (Aa), what
Park. After the fires, biologists
is the chance that their offspring
observed that new lodgepole pine
would have a heterozygous
seedlings began to sprout in the
genotype?
burned areas. The lodgepole pine
A. 0% cones are sealed with a resin that
B. 50% requires great heat to break open
and release seeds. What testable
C. 75% hypothesis could you form from
D. 100% this observation?
A. Lodgepole pine seeds sprout
easily.
B. Lodgepole pine is adapted to
frequent forest fires.
C. Lodgepole pine will grow
where other plants cannot.
D. Not all lodgepole pine seeds
are contained in a cone.
61 Grasses, hawks, mice, and snakes are all organisms that live in a prairie. In the
prairie food chain, which is the correct arrangement according to energy flow?
A. hawk → snake  mouse  grass

B. grass  snake  mouse  hawk

C. grass  mouse  snake  hawk

D. hawk  mouse  snake  grass

62 Sustainable agriculture is farming that conserves natural resources and helps keep the
farmed land productive indefinitely. This kind of agriculture minimizes the amount of
energy, water, pesticides, and fertilizer needed to grow crops. Sustainable agriculture
involves planting productive, pest-resistant crops that require fewer resources and less
maintenance. How do the resources needed for sustainable agriculture compare with
the resources needed for large-scale agriculture?
A. More resources are usually needed for the initial setup and planning of tradi-
tional large-scale agriculture techniques.
B. More resources are needed throughout the growing season for all crops grown
with sustainable agriculture techniques.
C. Fewer resources are needed for the initial setup and planning of sustainable agri-
culture, and less water, energy, and other costs will be needed over time.
D. More resources may be needed for the initial setup and planning of sustainable
agriculture, but less water, energy, and other costs will be needed over time.

63 The diagram below is a model of a cellular process called transcription.

TRANSCRIPTION

R R R R R R R R
DNA

C T T C
AGAA

G A
UC

mRNA
R R R R

What class of biological molecules is represented in the diagram above?

A. carbohydrates B. nucleic acids

C. lipids D. proteins
64 A sample of digestive juice was removed from the stomach of a pig. The juice was
placed in a test tube, along with some grains of wheat. A second, identical test tube
that contained an equal number of wheat grains was set up. However, pure water was
used rather than digestive juice. The test tubes were kept at 40°C, which is about the
temperature inside a pig’s stomach. After eight hours, the grains of wheat in the
digestive juice had broken apart into tiny particles. The grains of wheat in the water
were wet but otherwise unchanged. Which of the following is a valid conclusion from
this experiment?
A. Water does not play a role in the digestion of food in pigs.

B. Pigs can easily digest wheat but cannot digest other grains.

C. High temperatures increase the process of digestion in pigs.

D. Digestive juices help to break down food in a pig’s stomach.

Name Date

PRACTICE TEST 2
1 A scientist wants to investigate male-guppy behavior in the presence of female
guppies. She puts two longtailed guppies in the same fish tank. The male guppies
appear to be indifferent to each other’s presence. However, when the scientist adds
one female guppy to the tank, the male guppies become aggressive toward one
another. Throughout the experiment, the scientist gives the fish enough food so that
they do not need to compete for it. In the experiment, why does the scientist maintain
the abundance of food before and during the presence of the female guppy?
A. The scientist wants to see how the guppies react to various amounts of food.

B. The scientist wants to see if the female is more interested in food than the male
guppies.
C. The scientist wants the guppies to have enough energy to display their natural
behavior.
D. The scientist must ensure that the guppy’s behavior is not affected by the need to
compete for food.

2 It may seem obvious to people living in the modern world that disease is caused by
germs or pathogens, but germ theory took centuries to be developed and accepted.
Germ theory proposes that microorganisms are the cause of many diseases. This
theory was highly controversial when it was first proposed, but it is now a cornerstone
of modern medicine. Before germ theory, the view was that disease was spontaneously
generated. This ancient view of the cause of disease was first published more than
2,000 years ago. Spontaneous generation was first questioned in 1546, when Girolamo
Fracastoro proposed that diseases could be transferred from person to person
through small things like seeds. Germ theory was also supported by the observations
of Anton van Leeuwenhoek, who first examined pond water under a microscope and
identified microorganisms. Other scientists, such as Louis Pasteur, added more
evidence to the growing support for germ theory and led to innovations that changed
human society, such as antibiotics. What is one reason why germ theory was
controversial when it was first proposed?
A. Most of the public had little experience with diseases.

B. The previous theory that disease was spontaneously generated had only recently
been accepted.
C. The technology had not yet been developed to view microorganisms, many of
which can cause disease.
D. The previous theory that disease was spontaneously generated had immediate
and recognizable evidence.

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Name Date

3 A botanist has recently developed an enzyme called NoGro. This enzyme acts as an
herbicide by preventing cell growth in the leaves of a certain species of tree that is an
invasive plant species in Florida. The botanist wants to test the effects of temperature
on NoGro so that NoGro can be applied to the invasive tree under optimal
conditions on Florida farms. After looking into the background of the study, you
discover that the botanist performing this investigation is being paid by a company
that will make a lot of money if NoGro proves to be useful at eradicating the invasive
tree. How might this affect your view of the research in this experiment?
A. You should not consider this an important experiment, because it was funded
by industry and not a governmental agency.
B. You should approach the results of the experiment with skepticism and review
the experimental methods and results carefully.
C. You should ignore the conflict of interest between the botanist and the company
because all scientific endeavors are performed without bias.
D. You should immediately dismiss the entire experiment because the results were
definitely skewed to make NoGro look more effective than it actually is.

4 The graph below is from an experiment where total light exposure was adjusted for
three groups of rats. Each group had 12 rats and each group was exposed to different
amounts of light versus dark in a 24-hour period for three months. Food
consumption was measured in each group following each 24-hour period. Food
pellets are reported as means per group for three months. (Note: All rats used in the
experiment were the same age and breed. All were fed the same kind of food pellets.)
10.0
!NIMAL FOOD PELLETS (8100)

5.0

1.0
4:20 12:12 20:4
,IGHT 3CHEDULE LIGHT: DARK
(HRS)
What could you infer from the graph?
A. Light schedules affect the appetites of rats.
B. Rats eat more when they are exposed to more light.
C. The appetites of rats are unrelated to light schedule.
D. Rats eat more when they are exposed to higher temperatures.

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5 Darwin’s theory of evolution was criticized fiercely during his time. Modern scientists
now accept the theory while they still debate many of its details. Though a theory
cannot be “proven,” significant evidence can be used to support it. What kind of
evidence has led biologists to accept modern evolutionary theory?
A. comparing the sequence of DNA base pairs of different species

B. comparing different literary accounts of the origin of life on Earth

C. comparing the anatomies of purebred pets and other domesticated species

D. comparing Darwin’s knowledge with that of modern evolutionary scientists

6 Every year, monarch butterflies travel thousands of miles from their summer homes
in northern states and Canada to wintering spots in Mexico and southern California.
In spring, they make the journey back again. However, the butterflies die before they
reach their northern homes. New butterflies, hatched from eggs laid by migrating
monarchs, complete the journey. A scientist wanted to know how the new butterflies
found their way to their northern homes if they had never been there before. Which
of the following is not a testable hypothesis about monarch migratory behavior?
A. Monarchs can sense Earth’s magnetic field.

B. Monarchs are prettier than other types of butterflies.

C. Monarchs follow routes that have many milkweed plants.

D. Monarchs can smell the remains of the last season’s dead monarchs.

7 A theory is a set of related hypotheses that have been tested and confirmed many
times by many scientists. Which of the following could cause a long-held theory to be
challenged or even overturned by the scientific community?
A. new evidence that better matches the new theory than previous evidence

B. a popular celebrity who disagrees with the ideas outlined in the old theory

C. a scientist who has strong beliefs that the old theory is wrong but little evidence

D. a group of scientists who believe that new theories are usually better than old
theories
8 A newspaper headline says, “Detective Has Theory on How Computers Were Stolen
from Warehouse.” Which of the following words most accurately reflects the use of
the term theory in this newspaper headline?
A. experiment B. fact

C. hypothesis D. law

9 The careful observations of many scientists have resulted in the development of the
cell theory. Which of the following is not part of this theory?
A. All cells contain a nucleus.

B. All cells come from existing cells.

C. The cell is the basic unit of all living things.

D. All organisms are made up of one or more cells.

10 Sodium-potassium pumps, endocytosis, and exocytosis all involve active transport.

Which of the following is a characteristic of active transport?
A. It involves facilitated diffusion.

B. It requires energy from the cell.

C. It relies on vesicles that often function as pumps.

D. It moves substances with a concentration gradient.

11 The presence of which of the following could be used to distinguish between

prokaryotic and eukaryotic cells?
A. flagella B. cell wall

C. ribosomes D. mitochondria

12 Advancements in which of the following pieces of technology allowed scientists to

make new discoveries about materials on an atomic level?
A. telescope B. otoscope

C. light microscope D. electron microscope

13 AIDS is a fatal disease caused by HIV, a virus that attacks and destroys the
human immune system. Which of the following activities is a risk factor for
transmitting HIV?
A. sharing needles

B. shaking hands or kissing

C. being bitten by mosquitoes and ticks

D. coming in contact with infected toilet seats

14 Various types of cactuses, such as those shown in the picture below, thrive in
Arizona’s deserts even though there is little annual rainfall there.

Which adaptation listed below allows all types of cactuses to survive long periods
without rain?
A. waxy cuticle on their surface

B. deep roots that tap underground water

C. cessation of photosynthesis in summer

D. ability to take water from other desert plants

Name Date

15 The diagram below shows the major parts of the human brain.

A
C

B
D

What is part D?
A. cerebellum B. corpus collasum

C. midbrain D. pons

16 The picture below models a blood vessel carrying different components of blood.

If human blood did not contain component C, which function could you conclude
the blood incapable of?
A. clotting

B. fighting disease

C. carrying oxygen

D. having red color

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17 In the past, smallpox, which is caused by a virus, was a common and deadly disease.
Now, people no longer get smallpox. Which method eradicated the smallpox virus?
A. vaccination B. cooking food thoroughly

C. water purification programs D. more sanitary living conditions

18 A species of toad living in an area is similar in form to toad fossils found in very old
rock. Which hypothesis does this observation support?
A. Toads living near the area have evolved slowly over time.
B. Toads evolved rapidly, because there are many variations in form.
C. This species of toad burrowed into the rock and became fossilized.
D. The toads living today migrated to the area from a different region.

19 A new animal was discovered in a remote area of Southeast Asia. The animal seemed
to resemble a crocodile. Biologists have several ways that they can classify the mystery
animal and determine its evolutionary history. One way involves identifying impor-
tant characteristics of the animal and forming a hypothesis about the order in which
it evolved from a common ancestor. The first step in this process is to analyze the
characteristics in a data table.
ANIMAL CHARACTERISTICS
Backbone Lungs Mammary glands Bipedal
Fish Yes No No No
Deer Yes Yes Yes No
Human Yes Yes Yes Yes
Mystery animal Yes Yes No No

$EER (UMAN
&ISH

The cladogram above, based on the data in the table, could show the evolutionary
relationships of these animals. Where would a biologist place the mystery animal on
the cladogram?
A. before the fish B. between the fish and the deer

C. between the deer and the human D. after the human

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20 Today, biologists classify sponges as animals rather than plants. What evidence
supports today’s classification?
A. Sponges have asymmetry.

B. Sponges do not have a backbone.

C. Sponges cannot make their own food.

D. Sponges do not have tissues or organs.

21 Scientists used to group fungi with plants. Which of the following is a major factor
that determines why fungi are not classified as part of the plant kingdom?
A. Fungi do not have leaves.

B. Fungi grow close to the ground.

C. Fungi can reproduce either sexually or asexually.

D. Fungi are heterotrophs, but plants are autotrophs.

22 According to the primordial soup model, the first organic molecules could have
formed from simpler inorganic substances in Earth’s early oceans, but only if there
were a source of energy to cause such chemical reactions to take place. Which are
possible sources of energy that could have led to the production of these first
precursors to life?
A. lightning and cellular respiration

B. nuclear radiation and photosynthesis

C. photosynthesis and cellular respiration

D. solar radiation, volcanic eruptions, and lightning

23 Which species name meaning “wise man” describes hominid fossils associated with
the first known paintings?
A. Homo erectus

B. Homo habilis

C. Homo neanderthalensis

D. Homo sapiens
24 In a population of clover flowers, there are both white and purple varieties. The
cows that graze the field where the clovers grow prefer the purple variety. Over
time, the white clover flowers become much more numerous and the purple ones
more scarce. This is an example of what process?
A. evolution

B. reproduction

C. natural selection

D. genetic mutation

25 Immigration to the United States in the 1800s from Eastern Europe is an example of
which type of force for genetic change?
A. increased birth rate

B. genetic equilibrium

C. gene flow through interbreeding

D. increased chance of genetic mutation

26 Sexual reproduction increases genetic variation in a population. Which is a way that

meiosis and fertilization lead to genetic variation?
A. Alleles are recombined when gametes from different parents join together.

B. Mutations are usually repaired by cellular machinery before genes are expressed.

C. DNA is replicated when a fertilized egg becomes a growing embryo through mitosis.
D. Parents that are homozygous for the same trait will have offspring that are also
homozygous for that trait.

27 A parent that is heterozygous for two different traits (AaBb) can pass any combination
of two alleles (AB, Ab, aB, or ab) to its offspring. Which statement explains why this is
possible?
A. There are alternative versions of genes.

B. The two alleles for a single gene separate when gametes are formed.

C. For each inherited character, an individual has two copies of the gene.

D. The alleles of different genes separate independently of one another during

gamete formation.
28 The Punnett square below shows a cross between two rabbits. Black fur
(B) is dominant to brown fur (b).

B b

B 1 2
Bb � Bb
b 3 4

What would be the phenotype of the offspring indicated by box 3?

A. black

B. white

C. brown

D. a mixture of brown and black

29 During replication, a double-stranded DNA molecule opens up exposing the bases on

each strand. Complementary bases line up with the bases on each of the exposed
original strands, forming two new strands. The sequence of bases on one of the
original strands is CGGTAGGG. What is the sequence of bases on its
complementary strand of DNA?
A. CGGTAGGG

B. CGGTUGGG

C. GCCATCCC

D. GCCAUCCC

30 The base sequences below show two different sequences of the same gene.
Wild Type: TTGACTCGGTATAC
Mutant: TTGACTCGTATAC
What type of mutation is illustrated?
A. deletion

B. insertion
C. inversion

D. substitution
31 The diagram below shows the processes that occur during gene expression.
A

B C D

RNA
DNA

At which step would transfer RNA (tRNA) be necessary?

A. step A B. step B

C. step C D. step D

32 Cancer is one of the leading causes of death in the United States. Which of the
following best describes cancer?
A. an infection of foreign bodies called tumors
B. an uncontrolled growth and division of cells
C. an infectious cellular disease that you inherit
D. an individual’s immune system attacking itself

33 According to the genetic code, the mRNA codons UGU and UGC code for the
amino acid cysteine, the mRNA codon UGA is a stop codon, and the mRNA
codon UGG codes for the amino acid tryptophan. What would a mutation in the
second base of the codon UGA likely mean for the resulting protein?
A. The resulting protein would most likely be unaffected.
B. The resulting protein would most likely be unusually long.
C. The resulting protein would most likely be unusually short.
D. The resulting protein would most likely differ by one amino acid.

34 A consumer asked a genetic scientist whether genes from a genetically modified food
plant could possibly cause harm by incorporating themselves into human DNA.
What was the scientist’s most likely response?
A. No, because a person’s genome comes only from the sex cells of parents.
B. No, because genetically modified DNA can never be passed to offspring.
C. Too little is known about genetic engineering to be able to answer that question.
D. No, because humans have always consumed plant and animal DNA with no ill
effects.
35 The diagram shows a sperm cell. 36 The diagram below shows the
process of cell division.

A sperm cell needs lots of ATP,

which allows the tail to transport
it into the female reproductive
system. Which statement is true?
A. The sperm’s mitochondria Which of the following statements
are found in part A. about the process is correct?
B. The sperm’s mitochondria A. Two cells are produced, each
are found in part B. containing half of the DNA
C. The sperm’s mitochondria of the parent cell.
are found in part C. B. Four cells are produced, each
D. Sperm do not containing half of the DNA
contain of the parent cell.
mitochondria. C. Two cells are produced, each
containing a complete set of
the parent cell’s DNA.
D. Three cells are produced: one
cell receives the entire DNA of
the parent and the other two
synthesize new DNA from
spare nucleotides.
37 The following chart shows the number of 2n chromosomes in various organisms.

Organism Number of 2n Chromosomes

Mosquito 6
Corn 20
Human 46
Dog 78
Which series lists the number of chromosomes in each gamete of a mosquito, corn,
a human, and a dog?
A. 3, 10, 23, 39

B. 3, 15, 22, 46

C. 6, 20, 23, 39

D. 6, 20, 46, 78

38 Cells and the organisms they make up reproduce through cell division. Some
organisms reproduce through mitosis, while others reproduce through meiosis and
fertilization. What advantage does meiosis give to organisms that reproduce sexually?
A. Meiosis ensures that offspring inherit genes from their parents.

B. Meiosis ensures that offspring will not inherit any genetic disorders.

C. Meiosis ensures that offspring are genetically different from their parents.

D. Meiosis ensures that offspring will have identical phenotypes to their parents.

39 Rivers and the aquatic life that live within them can change over time due to human
activity and natural forces. Which of these changes would likely have the greatest
effect on a river system?
A. A city being built near the mouth of the river.

B. A bridge that crosses the river high in the mountains.

C. A change in the salinity of the ocean into which it flows.

D. A dam on the river near where it flows from the mountains.

40 Tropical rain forests receive as much as 450 cm of rain per year. They are the richest
biome in terms of number of species. Which statement best explains how destruction
of tropical rain forests could affect the carbon cycle?
A. Destroying rain forest trees would cause carbon dioxide levels in the atmosphere
to drop dangerously low.
B. A loss of rain forest trees would have only a small effect on the carbon cycle,
because they are green all year long.
C. Rain forest destruction would cause only a small increase in carbon dioxide
levels if the roots were left to remove carbon dioxide from the air.
D. Cutting down rain forests could increase atmospheric carbon dioxide levels,
because trees that could take up carbon dioxide would be removed.

41 The United States experienced a rapid growth of its population in the early
1900s. Which of the following factors did not contribute to this rapid
population growth?
A. Immigration was greater than emigration.

B. The birth rate was higher than the death rate.

C. More people moved from farms to large cities.

D. Advances in technology led to cleaner food and water supplies.

42 Noxious weeds are weeds that invade ecosystems and grow very quickly and
aggressively. In Colorado, more than 1 million acres are affected by noxious weeds.
How do noxious weeds affect the biodiversity of an ecosystem?
A. The biodiversity increases slightly because they represent another species in the
area.
B. They increase the biodiversity because they increase the total energy of the
producers.
C. The biodiversity usually decreases greatly as the noxious weeds outcompete the
local plants.
D. The biodiversity is not affected at all since the noxious weeds simply replace the
dominant plant in the ecosystem.
43 The diagram below is an energy pyramid.

In what level is there the least energy available?

A. the level that contains rats

B. the level that contains grasses

C. the level that contains the owl

D. the level that contains grasshoppers

44 A renewable resource is a natural resource that can be replaced at the same rate at
which the resource is used. Which of the following is true of renewable resources?
A. They are less useful than nonrenewable resources.

B. Many of them can become scarce if used too quickly.

C. They must be converted into nonrenewable resources.

D. No matter how much we conserve, they will one day be gone.

45 There is a general pattern in how environmental workers and activists overcome

environmental problems. Which lists five steps of solving an environmental problem
in the order in which they happen?
A. assessment, public education, political action, risk analysis, follow-through

B. assessment, risk analysis, public education, political action, follow-through

C. follow-through, assessment, public education, political action, risk analysis

D. public education, risk analysis, assessment, follow-through, political action

Name Date

46 In 1985, a researcher in Antarctica 48 Photosynthesis allows plants

noticed that ozone levels in the to produce most of the organic
atmosphere seemed to be as much molecules they need. What is the
as 35 percent lower than the primary source of energy for
average values during the 1960s. photosynthesis?
Satellite images taken over the
South Pole revealed that the ozone A. ATP
concentration was unexpectedly B. fermentation
lower over Antarctica than
C. heat
elsewhere in the Earth’s
atmosphere. It was as if an “ozone D. sunlight
eater” were causing a mysterious
zone of below-normal
concentration, an area that
researchers called the ozone hole. 49 Which of the following pairings
Which human activities are shows the substance released
thought to be responsible for during cellular respiration and the
creating this ozone hole in the cellular structure that is
upper atmosphere? responsible for that process?
A. increasing the human A. O2 and chloroplast
popula- tion and cutting
B. ATP and chloroplast
down forests
C. CO2 and mitochondria
B. burning fossil fuels and the
related increase in atmospheric D. glucose and mitochondria
CO2
C. burning sulfur-rich coal and
increasing levels of greenhouse
gases 50 In the carbon cycle, cellular
respiration is the process
D. producing and releasing
responsible for the conversion of
chemicals called
carbon-containing sugars into
chlorofluorocarbons (CFCs)
carbon dioxide gas, which can then
become part of the atmosphere.
What process can remove this
gas from the atmosphere and
47 What are the subunits that make
return it to the biosphere?
up complex carbohydrates?
A. fermentation
A. amino acids
B. glycolysis
B. fatty acids
C. photosynthesis
C. monosaccharides
D. transpiration
D. nucleotides

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Name Date

51 Frank made the drawing of ATP shown below.

Base (adenine)
Phosphate groups

P P P

Sugar
(ribose)

How is this molecule most often involved with the production of energy in a cell?
A. Energy is released when the third phosphate group breaks off.

B. Energy is produced when a fourth phosphate group binds to the molecule.

C. Energy is produced when the sugar ribose is metabolized in the Krebs cycle.

D. Energy is released when the adenine base is used to form part of an RNA
molecule.

52 The graph below shows the reaction rates of two different reactions.

45
40
35
30
25
20
15
10
5 Reaction A
Reaction B

0
123456
Time (msec)

Which of the following statements could explain the difference in the two reactions?
A. Reaction A is catalyzed by an enzyme.

B. Reaction B is catalyzed by an enzyme.

C. Reaction A occurs at a faster rate than Reaction B.

D. Reaction A and reaction B have the same reaction rate.

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Company Florida Biology Standards Review
181
53 Water is often called the “universal solvent” because it can dissolve many different
kinds of substances. Which statement best explains why fatty acids do not dissolve in
water?
A. They are organic molecules.

B. They are nonpolar molecules.

C. They have a long chain-like structure.

D. They are made up of hydrogen, carbon, and oxygen.

54 A student places a bunch of grapes in a bowl of plain water and a second bunch
of grapes in a bowl of salt water. After an hour, the grapes in the plain water are
swollen, whereas the grapes in the salt water are shriveled. What cellular process
caused the grapes to shrivel?
A. active transport B. diffusion

C. exocytosis D. osmosis

55 Modern scientists have observed that genetic changes happen over time in all
natural populations. Therefore, by comparing amino acid sequences, scientists can
determine how similar one species is to another. The table below compares amino
acids in a number of species.

Hemoglobin Comparison
Animal with Amino acids that differ
hemoglobin from human
hemoglobin
Gorilla 1
Rhesus monkey 8
Mouse 27
Chicken 45
Frog 67
Lamprey 125

Based on the information in the table, which animal is most closely related to
humans?
A. chicken B. gorilla

C. lamprey D. rhesus monkey

56 The Hardy-Weinberg principle states that the frequencies of alleles in a population
do not change unless evolutionary forces act on the population. According to this
principle, which of the following is not a factor in bringing about a change in the
frequency of alleles and genotypes in a population?
A. gene flow B. genetic equilibrium

C. mutation D. natural selection

57 Examine the illustrations of the two bird feet shown below.

In what types of environments would such feet have a selective advantage?

A. pine and fir forests B. water and wetlands

C. mountains and rocky cliffs D. deserts and deciduous forests

58 A trait for flower color shows incomplete dominance, where heterozygous individuals
have pink flowers. If a homozygous individual were crossed with a heterozygous
individual, what percentage of their offspring would exhibit pink flowers?
A. 0% B. 50%

C. 75% D. 100%

59 Genetic engineering involves both ethical and scientific issues. Which of the following
is not an ethical factor in pursuing genetic research?
A. debate over the wisdom of allowing patents for specific genes or gene sequences
B. concern that a genetically modified organism could cause unforeseen environ-
mental harm
C. debate over whether research on introns or research on transposons
should receive the most funding
D. concern about who should be allowed to have information about the
results of genetic tests on individuals
Name Date

60 The graph below shows the percentage of seeds that germinate after being in cold
storage for a varying number of days.

Effect of Cold Storage

100

Percent germination
80

60

40

20
0
0 1 20 30 40 50 60 70
0
Number of days at 4
˚C

Some plants require a cold period to break seed dormancy. What would likely
happen to seeds that usually experience a period of dormancy in cold weather if
they are planted in a tropical climate?
A. The seeds would not germinate.

B. The seeds would germinate very quickly.

C. The plants that grew from the seeds would have larger flowers.

D. The flowers of the new plants would not produce any more seeds.

61 The food web below represents the interactions between organisms in a salt marsh
ecosystem and organisms in an old field ecosystem.
FOOD WEB
Coyote Hawk

Shrew
Mouse Duck Heron

Fish
Grasshopper Brine shrimp

Plants Algae and phytoplankton Crab

Which group of organisms is missing from this diagram?

A. Consumers are missing from this diagram.

B. Producers are missing from this diagram.

C. Decomposers are missing from this diagram.

D. All the different types of organisms are included.

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184
62 The International Panel on Climate Change is a group of scientists from many
countries who study and monitor the temperature of Earth’s atmosphere. The
panel has concluded that Earth’s climate is growing steadily warmer. The reason
for this global warming, they contend, is an increase of carbon dioxide in the
atmosphere, resulting from the burning of fossil fuels in power plants, factories,
and automobile engines. Carbon dioxide is a greenhouse gas that causes heat to
build up in the atmosphere. Although there are seasonal variations in the amount
of atmospheric carbon dioxide, the panel reports that overall atmospheric levels
have been steadily rising since the late 1700s, when the Industrial Revolution
began. The graph below shows how atmospheric carbon dioxide concentration
varies over a year.

!TMOSPHERIC #ARBON $IOXIDE 6ARIATION

(PARTS PER MILLION) #ARBON DIOXIDE CONCENTRATION

358
356
354
352
350
348
346
344
*AN. -ARCH -AY*ULY3EPT..OV.
-ONTH

How can the dip in carbon dioxide levels shown on the graph be related to the carbon
cycle?
A. Fewer trees are cut for firewood during warmer months.

B. Erosion of farmland removes carbon dioxide from the air.

C. More carbon dioxide is dissolved in lake and ocean waters during the warm
summer months.
D. Increased plant growth and photosynthesis during summer months remove more
carbon dioxide from the atmosphere.
63 Which of the following statements is true?
A. Water molecules gain energy during evaporation.

B. Water molecules lose energy during evaporation.

C. Water molecules lose energy during precipitation.

D. Water molecules gain energy during condensation.

64 Enzymes catalyze chemical reactions that keep cells alive. Imagine that a cell had no
enzymes. How would having no enzymes affect the chemical reactions in the cell?
A. They would happen too slowly to support cellular processes.

B. They would happen too rapidly to support cellular processes.

C. They would happen at the same rate as they do with enzymes.

D. They would happen normally, only they would use different reactants.
Name Date

EOC Practice Test 1 – ANSWER SHEET

1. 17. 33. 49.

2. 18. 34. 50.

3. 19. 35. 51.

4. 20. 36. 52.

5. 21. 37. 53.

6. 22. 38. 54.

7. 23. 39. 55.

8. 24. 40. 56.

9. 25. 41. 57.

10. 26. 42. 58.

11. 27. 43. 59.

12. 28. 44. 60.

13. 29. 45. 61.

14. 30. 46. 62.

15. 31. 47. 63.

16. 32. 48. 64.

187
Name Date

EOC Practice Test 2 – ANSWER SHEET

1. 17. 33. 49.

2. 18. 34. 50.

3. 19. 35. 51.

4. 20. 36. 52.

5. 21. 37. 53.

6. 22. 38. 54.

7. 23. 39. 55.

8. 24. 40. 56.

9. 25. 41. 57.

10. 26. 42. 58.

11. 27. 43. 59.

12. 28. 44. 60.

13. 29. 45. 61.

14. 30. 46. 62.

15. 31. 47. 63.

16. 32. 48. 64.

189