Kruss Manual-Dsa100
Kruss Manual-Dsa100
Contents
2 Introduction .................................................................................................... 8
4 Computer system......................................................................................... 11
4.1 Computer requirements ................................................................................................... 11
4.2 Mounting a frame grabber board (for CF3200/3201 and CF3220/21) .............................. 12
4.3 Driver installation ............................................................................................................. 13
5 Setting up..................................................................................................... 22
5.1 Opening the transport packing and checking the scope of supply ................................... 22
5.2 Transport.......................................................................................................................... 22
5.3 Location ........................................................................................................................... 23
5.4 Levelling the instrument ................................................................................................... 23
5.5 Power supply ................................................................................................................... 24
5.6 Connecting the computer ................................................................................................. 25
8 Single-Dosing units...................................................................................... 54
8.1 Manual single-dosing unit DS3200/3201.......................................................................... 54
Appendix.......................................................................................................... 99
1 General information
As well as this general safety information, please follow the safety instructions in the
remainder of the manual text.
The DSA100 is intended for use in a laboratory or pilot plant station. The general safety
rules for working in a laboratory environment apply to work carried out with the
DSA100.
When carrying out measurements with substances which are poisonous, hazardous to
health, irritant and/or easily inflammable, the safety regulations and, if available,
information in the appropriate safety data sheets must be taken into account when
handling such substances.
The controls of the DSA100 and a connected computer must not be operated when
wearing protective gloves, which have already been used for handling hazardous
materials.
Disconnect the DSA100 from the power supply immediately if there is any damage to
the electronics, if liquids are spilled over the DSA100 or if there is an escape of
tempering medium.
ATTENTION Handle all glass parts and optical parts with care. Do not touch them with fingers or with
sharp objects.
page 6 General information
• The DSA100 is intended only for measurements of the surface tension of liquids,
the interfacial tensions between two liquids and measurements of the contact angle
between a liquid and a solid.
• The intended use includes the exclusive use of accessories supplied by KRÜSS or
substitutes with an identical design if disposable accessories are being used.
• The components of the DSA100 are not resistant against acids, bases or organic
solvents. KRÜSS does not provide for damages due to spilling any of these
substances. The same applies to damages due to corrosive vapours.
Contact KRÜSS
* If you did not obtain the measuring instrument directly from KRÜSS but from a
dealer, please contact your dealer first. He may be able to rectify the damage. If
not, contact KRÜSS customer support directly.
You will be given a repair number. Please quote this number in your covering letter
when you dispatch the unit.
* Components which are not directly affected by the damage may also have to be
sent with the unit. KRÜSS customer support will advise you on the necessary
scope of the shipment.
! Remove liquids (e.g. samples, temperature control liquid) completely from the
instrument and the accessories.
! Clean the unit or accessories before dispatch. This applies particularly if there is
contamination due to hazardous substances.
General information page 7
* If you no longer have the original packaging, KRÜSS can provide this at the
customer’s expense.
! Pack all components separately which were packed separately when the unit was
supplied. Do not dispatch the unit in the assembled state.
! If the instrument or accessories were provided with transportation locks, refit these
before shipping.
* Our customer support department will be pleased to advise you regarding the
proper packaging.
If problems should occur when working with the DSA100 or with DSA1/DSA3, please
consult the technical customer support at KRÜSS GmbH. You can contact them at:
KRÜSS GmbH
Technical support
Borsteler Chaussee 85-99a, D-22765 Hamburg (Germany)
Tel:(+49) 040/514401-0
Fax:(+49) 040/514401-98
E-mail: [email protected]
page 8 Introduction
2 Introduction
Thank you for deciding to purchase a DSA100 Contact Angle Measuring System. With
the DSA100 and the DSA control and evaluation software you now have a tried and
tested tool for carrying out computer-supported studies into the shapes of drops of
liquid. In addition to the high-precision dosing and positioning of the liquid drops the
system also permits the recording and evaluation of video images and the calculation
of surface energies and surface tension with the aid of the numerous evaluation
methods that have been implemented.
! This manual provides instructions for the installation of the instrument and the
exchange of components. We strongly advise you not to carry out the installation
and necessary assembly work without consulting this manual. Even if you treat the
instrument carefully, errors could inadvertently occur unless you are fully aware of
its properties; these could result in incorrect measurements, damage to the
instrument or even injury.
* The system is characterized by its high degree of flexibility. All the components –
movement axes, video system, dosing unit, environmental chambers – can be
easily exchanged or extended. All intermediate stages are possible from fully
manual operation up to PC-control of all the components.
The DSA100 measuring system – depending on the hardware and software supplied –
opens up the following possibilities:
• Integrated and extendable databases with substance data on liquids that can be
directly linked to the measuring data
3 DSA100 overview
In order to measure the contact angle with the DSA100 a drop is placed on a sample
located on a moveable sample table. The drop is illuminated from one side and a
camera at the opposite side records an image of the drop.
• The sample table with up to three mobile axes or a rotating wafer table
• The video system with camera, optical system, prism, light source and aperture
Dosing unit
Camera
Aperture
Prism tilt
Illumination
Prism
y-axis
Sample
table
(z-axis)
x-axis
Computer system page 11
4 Computer system
If you have purchased your computer from KRÜSS together with the DSA100 and if the
frame grabber board has already been installed then you can skip this chapter.
The following is a list of the minimum requirements to enable the DSA software to run
without any problems:
• CD-ROM drive
• Mouse
• Colour monitor
• Printer (optional)
CF3200/3201
• Windows XP or Vista
CF3216
• Interface IEEE-1394b
• Windows XP or Vista
page 12 Computer system
CF3220/21
• Free PCI-X (64 bit), PCI (32 bit) or PCIe X4 slot (all full length)
• Windows 2000 or XP, with MS .NET framework v2.0 and MDAC v2.6
DANGER X Switch off the computer and pull out the mains plug before opening the
computer housing.
! Mounting the board must be carried out before installing the DSA software at all
circumstances.
Please install the board in exact accordance with the following instructions. If
necessary, obtain the help of a computer specialist.
1. Switch off the computer and pull out the mains plug. Open the computer housing.
2. Remove the board from the packaging and insert the board in a free PCI slot.
3. Screw the board with its fixture onto the computer housing.
X Deactivate the automatic driver installation before assembling the board (see
below).
1. Select the Control Panel in the Windows start menu and open the configuration
menu System .
3. Select the option Never search Windows Update for drivers and click on OK.
* Later, when the installation process is finished, the option Ask me to search…
can be selected if this Microsoft service is desired.
After switching-on the PC the Windows hardware assistant detects the new board. The
assistant for driver installation starts.
* All drivers required for installation are located on the DSA1/DSA3 CD.
* After installing the frame grabber the DSA1/DSA3 software can be installed.
Further information is given in the DSA1/DSA3 manual.
Let the assistant search for the drivers on the DSA1/DSA3 CD. The drivers will be
installed automatically after confirmation with OK.
Computer system page 15
First close all running programs; several re-boots are necessary during the
installation process.
CAUTION X Carry out the installation in exact accordance with the following instructions.
* PFRemote is software for camera control. KRÜSS has worked out standard
parameters for the high-speed camera which are transferred to the DSA3 software
with the help of prepared parameter files. (Ö DSA3 manual, chapter about high-
speed cameras.)
4. Skip the following information page about different camera models with Next.
6. Confirm the installation of the two components PFRemote and SDK with the
option Without USB Environment .
7. Confirm the proposed start menu folder with Next or select a custom-defined folder
with Browse . With Don’t create the creation of a start menu item can be obviated.
8. In the next steps short cuts can be created on the desktop or in the Windows quick
launch bar. Confirm your selection with Next .
10. Before finishing, the setup program recommends reading two information texts.
Conclude the installation with Finish.
Computer system page 17
1. Select the folder CF3220 on the DSA3-CD and start the program setup.exe .
2. Confirm the installation of the two components Mil Runtime and Intellicam with
Next.
4. Set a tick to the box for the Solios board and confirm with Next .
page 18 Computer system
5. Leave the default settings on the following page unchanged and confirm with Next .
6. Confirm your license agreement and click on Next . Ignore the possible warning
that the Windows Logo Test was not passed and continue with the installation.
1. Select the folder CF3220 \Mil Lite Patch on the DSA3-CD and start the
containing .exe program.
2. Click on Next in the welcome window to start the installation. A text pops up and
informs you about the changes.
1. Start the program Intellicam from the start menu folder Matrox Imaging .
Computer system page 19
3. After the upgrade the computer switches off automatically. Reboot the computer.
1. Start the program MilConfig from the start menu folder Matrox Imaging ÆTools .
3. Move the slider two tick marks to the left. The text Disable DDraw Overlay for
mono images must appear in the lower left of the scale.
4. Click OK .
page 20 Computer system
! The IEEE 1394 driver should be installed before installing the DSA1/DSA3
Software under all circumstances.
2. Open the folder SUPPORT Æ Cameras Æ AxF . Open the containing folder
named after the version number of the installation program. Start the program
AVTFirePackage_xxx.exe .
4. A warning message appears that installing the AVT driver blocks the IEEE 1394
interface for other drivers. Click on Next .
6. A dialogue box containing installation options appears. Normally, you can use the
default options. Click on Next .
* If several IEEE 1394 boards are installed and not all of them shall work together
with the AVT driver: Select the option for selected 1394 cards . A dialogue for
selecting the board(s) to be used by the AVT driver will open in the course of
the installation process.
* IEEE 1394 boards that are not used should be deactivated in the Windows
control panel.
7. Start the installation with a further click on Next . If necessary, confirm the
continuation of the installation in the warning message (Windows Logo-Test ).
5 Setting up
5.1 Opening the transport packing and checking the scope of supply
Open the transport packing Check the DSA100 and its accessories for damage
during shipment.
Please contact KRÜSS or your local dealer without delay if damage during
shipment occurred or if the supply is incomplete.
5.2 Transport
! Remove parts which lie on the DSA100. Take care that all components are fixed.
Transport the DSA100 with the help of a trolley. Hold the unit firmly during
transportation.
To lift the DSA100 onto the trolley and from the trolley to the place of use: Hold the
unit from both sides under the base plate.
! Do not hold the unit at the illumination, a dosing unit or an axis for
transportation.
Setting up page 23
5.3 Location
• Place the instrument on a stable laboratory bench in a quiet location. The floor
should be constructed so that it does not transmit vibrations. Vibrations caused, for
example, by slamming doors or passing personnel should also be avoided.
• Do not place the instrument in the vicinity of a bright source of light; under no
circumstances should it be placed in front of a window.
• Avoid drafts during the measurements. Do not place the instrument in the vicinity of
cooling or heating systems or ventilation units.
* If not all axes have yet been assembled this step must be carried out before
levelling the instrument (Ö ch7).
For levelling, two spirit levels are located on the bottom of the standard sample table
support.
* For some special sample tables, e.g. a wafer table, an external spirit level must be
put on top of the sample table.
2. Remove the two clips (if present) from the sample table support.
page 24 Setting up
4. Adjust the height of the four feet of the measuring system until the air bubbles in
the two spirit levels are located between the markings in the middle.
Connect the round 4-pin plug to the “24V DC” socket on the rear panel of the
DSA100.
* The main switch “Power” is beside the connection. After the instrument has been
switched on all the software-controlled axes, the needle selector and the syringe
drives (if present) move to their zero positions for initialization.
* When the instrument is switched on the LED on the front panel lights up to show an
intensive blue, when the instrument is switched off it is still weakly lit up.
Setting up page 25
Connect the RS232 plug (“Serial PC”) at the DSA100 rear panel with a serial
interface of the computer.
After the start of the software the serial port to which the DSA100 is connected must be
entered.
Assignment in DSA1
Assignment in DSA3
Select the occupied COM-port from the drop-down menu marked above.
A protective glass to be attached to the front panel is supplied with the instrument.
The protective glass prevents accidental contact with the needle tip to avoid
injury or poisoning risks. This protective glass should only be removed when
conversion work is being carried out.
Setting up page 27
The instrument comes with a camera already assembled in the camera housing of the
DSA100. The camera must be connected to the frame grabber board of the computer.
CF3200/3201
Connect the “Video” connection on the rear side of the camera to the frame-
grabber board of the computer. Use the supplied BNC cable.
CF3220
Connect the cable with the Camera Link plug (coming from the DSA100 panel) with
the connection “Channel 0“ of the frame grabber board.
IEEE-1394 cameras
! The prism is a sensitive component and must therefore be built in after delivery. Do
not touch the prism glass with your fingers or sharp or pointed objects.
Insert the prism in the opening and push it upward until it clicks into position.
* The prism can be tilted in order to change the viewing angle of the drop. The
advantage of this arrangement is that the position of the drop image does not alter
and the image itself is not distorted.
* The tilting angle is regulated by using the rotary knob beside the prism. When the
instrument is switched on the scale on the left-hand side of the housing shows the
set tilt angle.
* The default settings are +2° for sessile and 0° for pendant drops.
Setting up page 29
The light slit can be narrowed in order to prevent disturbing reflections at the upper
side of the drop (Ö DSA software manual). To do this, pull the slider downward.
aperture slider
On the base of the illumination module there are two connectors for connecting a
temperature sensor.
Connect the temperature sensor to the rear socket (the following image shows the
rear side).
page 30 Exchanging cameras
6 Exchanging cameras
! Before changing a camera the drivers required for its use must be installed.
6.1 Assembly
3. Remove the covers from the camera housing. In order to do that, pull the spring
inside the chamber and lift up the cover.
* For CF3220/21 and for IEEE-1394 cameras the camera cover has an outlet for
the cables at the rear side. Exchange the camera covers when changing from
CF3200/3201 camera to one of these cameras.
Exchanging cameras page 31
4. Remove the connections from the installed camera (image: standard camera).
image transfer
power supply
5. Loosen the screw marked in the following image using an allen wrench (1.5 mm)
and remove the camera from the guidance.
7. Put the protective cap on the removed camera and keep it save from dust.
8. Screw the tube onto the new camera. Insert the camera into the guidance.
9. Connect the power supply and the port for image transfer:
2. Place an object with an exactly horizontal edge onto the sample table and get the
edge into focus (Ö DSA1/DSA3 manual).
* You may use the horizontal measuring lines in the DSA software as a support
(Ö DSA1/DSA3 software manual).
* Usually the axes are already assembled when the instrument is delivered. This
chapter describes the assembly of axes or tables that were acquired afterwards.
* If one ore more software-controlled axes are to be assembled and only manual
axes are currently installed, the additional installation of an interface board is
necessary. In other cases the chapters 7.1 and 7.2 can be skipped.
Switch off and unplug the DSA100 before starting with the installation.
1. Open both doors of the chambers on both sides of the DSA100. If necessary,
remove the liquid containers and their racks from the chambers.
2. In both chambers, a hexagon socket screw is situated behind the stand. Screw out
the screws in both chambers.
page 34 Assembling axes and tables
4. Unscrew the middle cover. It is fixed with two hexagon socket screws on the rear
side and two screws on the inside.
5. Place the interface board in the middle compartment, lead the connecting cables
through the gap to the compartment on the left-hand side. Use the right-hand
connection to plug the board.
6. Fix the plate with the axes connections with two screws each at the rear side and
the inside of the compartment.
Assembling axes and tables page 35
7. Press the interface board to the stop into the free socket on the lower right (seen
from the rear side).
! Take care that the whole width of the socket is used for the board so that all
poles are occupied. If the board is not properly connected it can be destroyed
when switching on the DSA100.
...with the screw to the bottom of the compartment so that the edge of the board is
situated in the gap of the supporting block.
page 36 Assembling axes and tables
9. Occupy the sockets of the installed board with the four plugs coming from the
connecting plate. The connections for axes 1 to 4 are plugged into the board in
ascending order from right to left.
Axis 4 Axis 1
Axis 2
Axis 3
10. Reverse the steps 2 - 4 to reassemble the cover and to put the instrument into an
operational state.
The connections for the axes are located on the rear panel of the DSA100.
The x-axis (left to right), the z-axis (up and down), a wafer table or the tilting table can
be connected to any of ports 1, 3 and 4; the axes are recognized automatically.
! Port 2 is reserved for the y-axis (front to back). If the y-axis is connected to a
different port then it will be recognized by the system as being the x-axis!
The different arrangements of the axes result in different total heights. In order to
ensure that the sample table is always at the height of the optical axis distance pieces
(“spacers”) must be attached or removed during the assembly of the axes.
Three different spacers can be supplied with the instrument: 36, 49 and 65 mm.
Depending on the particular order, one or more spacers may have already been built
in.
Each of the spacers has three threaded holes for screwing onto a different spacer and
four threaded holes at the corners for screwing an axis onto the spacer:
page 38 Assembling axes and tables
The following table shows which spacers should be used on the frame of the DSA100
for the various combinations of axes and environmental chambers.
* In special cases, for example to adapt the system to large samples, the sample
table support can be removed or a different combination of spacers be assembled.
Assembling axes and tables page 39
! For safety reasons first remove the prism and keep it in a safe place when carrying
out the assembly work.
1. If axes have already been installed, move them so that the attachment screws are
exposed.
* With software-controlled axes: please consult the DSA software manual to see
how the axes are moved.
3. Screw off the four screws that connect the lowest axis to the spacer (4 mm allen
wrench).
! Hold the axis firmly with your other hand so that it cannot fall to the ground.
4. Remove the clips that are attached with the screws and remove the axes from the
spacer.
5. If the attached spacer has to be removed, loosen the four screws used to attach the
spacer to the DSA100 frame.
6. Screw the required spacer(s) to the DSA100 frame or to a spacer that is already in
position.
page 40 Assembling axes and tables
z
y
x
Spacer
Each axis is either screwed onto a spacer or onto another axis. To assemble an axis,
attachment strips are required as follows:
• Four strips, two with and two without threads, for attaching a y-axis to an x-axis
Attachment Attachment
strips with strips without
thread thread
* If only the z-axis is present then no attachment strip is required; the axis is screwed
directly onto the spacer.
! Not always are all strips used which were delivered. Keep the unneeded strips save
for the occasion of further upgrades.
Assembling axes and tables page 41
1. Place the axis on the built-in spacer and hold it firmly. The axis should be
positioned so that the spacer is located below the centre of the range of movement
of the axis.
2. Place the non-threaded attachment strips on the spacer so that the cramping edges
fit inside the ridges of the axis.
cramping edge
3. Slide the strip along the axis so that the openings in the strips and spacer lie above
each other.
! Tighten up the screws in sequence to achieve uniform stress and avoid tilting.
page 42 Assembling axes and tables
1. Place the y-axis on the x-axis. Place one of the attachment strips with threaded
openings below the y-axis so that the cramping edges fit inside the ridges of the x-
axis. Hold the strip firmly.
2. Place two non-threaded attachment strips with their cramping edges inside the
ridges of the y-axis. Slide the strips so that the openings on the upper strip coincide
with threaded openings of the lower attachment strip.
Fix the strips with two screws lightly so that the axis can still slide about.
3. Treat the other side of the axis in the same manner. Slide the y-axis so that it lies
as centrally as possible above the x-axis.
! Tighten up the screws in sequence to achieve uniform stress and avoid tilting.
Assembling axes and tables page 43
Assembly on a spacer
If only the z-axis is used then this is screwed directly onto the spacer without any
attachment strips.
! Tighten up the screws in sequence to achieve uniform stress and avoid tilting.
Assembly on an x- or y-axis.
2. Place one of the threaded attachment strips below the z-axis so that the cramping
edge fits inside the ridge. Slide the strip and the z-axis until the openings of the z-
axis coincide with the openings in the strip.
3. Fix the strip lightly so that the axis can still slide about.
5. Slide the axis to the central position above the lower axis.
! Tighten up the screws in sequence to achieve uniform stress and avoid tilting.
page 44 Assembling axes and tables
The wafer table cannot be used together with the x-axis, but only as an alternative to it.
If an x-axis is already attached then it must be removed before assembling the wafer
table.
! For safety reasons first remove the prism and keep it in a safe place when carrying
out the assembly work.
1. If software-controlled axes are installed then switch on the computer and start the
DSA software.
2. With manual axes: wind the axis to the back until the screws are exposed.
With software-controlled axes: Select the register card Axis respectively Axes in
the Device Control panel.
DSA1 DSA3
Select the y-axis with the button at the top left and use the Up button to move the
axis until the screws are exposed. A further click on the button stops the
movement.
4. Loosen the screws beneath the sample table support. Remove the sample table
support from the z-axis.
! Place a cloth over the rear side of the DSA100 frame in order to prevent
scratches during the further steps.
5. All the axes are fastened with attachment strips and screws. Remove the
attachment screws for the y-axis (3 mm allen wrench).
9. Fix the attachment strips with four screws lightly so that the axis can still be moved.
Move the whole axis so that the spacer is located in the centre of the range of
movement of the axis.
! Tighten up the screws in sequence to achieve uniform stress and avoid tilting.
Assembling axes and tables page 47
Place the wafer table on the sample table. The wafer table must be attached with
four hexagon socket screws (4 X 8 mm). Rotate the wafer table manually and use
the gap in order to screw in the screws.
In case of a software-controlled wafer table: Switch off the DSA100. Connect the
wafer table with one of the ports Axis1, Axis3 or Axis4 on the rear panel of the
DSA100. Switch on the DSA100. Close the DSA software and then start it up
again. Call up the menu command Reset Zero Position from the tab sheet Axis
respectively Axis in the Device Control panel.
page 48 Assembling axes and tables
CAUTION X Before driving the wafer table upward, check if there is enough space
between the prism and the wafer table.
X Move the table slowly and do not use the movement mode Continuous in the
DSA software.
! The prism must be adjusted to a tilting angle of 2° for sessile drops (Ö ch. 4.6).
* The large wafer table is to be used together with the long y-axis PS3210. A
currently assembled shorter y-axis must be exchanged.
* The large wafer table can be assembled easier when a dosing system is already
attached to the DSA100.
2. Attach the rotating axis to the platform of the z-axis using four hexagon socket
screws.
3. Attach the platform to the rotating axis using four hexagon socket screws.
Assembling axes and tables page 49
For software-controlled y-axis: Connect the y-axis to the port AXIS 2 of the rear
panel of the DSA100 and switch on the DSA100. The y-axis initializes and drives to
the front position automatically.
5. Loosen the four screws that fix the y-axis so that the whole y-axis can slide forward
and backward manually.
6. Rotate the sample table so that the maximum length is aligned to the y-axis (i.e. the
pointed ends show to the front and the rear side).
7. To achieve the whole driving rage of the axis, push the y-axis to a position where
the needle of the dosing system reaches the outermost position of the sample
platform.
* The axis can also be adjusted to the central position. This can be done to make
the DSA100 better accessible from the front side, but only if there is sufficient
space behind the DSA100.
page 50 Assembling axes and tables
9. In case of a software-controlled wafer table: Switch off the DSA100. Connect the
wafer table with one of the ports Axis1, Axis3 or Axis4 on the rear panel of the
DSA100. Switch on the DSA100 again. Close the DSA software and then start it up
again. Call up the menu command Reset Zero Position from the tab sheet
Axis/Axes in the Device Control panel.
Assembling axes and tables page 51
If high movement speeds are set for the control of the axes then an unexpectedly rapid
movement of the Tilting Table could cause injuries.
WARNING X Before using the Tilting Table set the movement speed for the axes to
medium or slow (Ö DSA software manual).
When working with a multi-dosing system liquids can escape when the table tilts. This
could represent a risk depending on the hazard class of the liquid.
CAUTION
X Fill the storage bottles with max. 10 ml test liquid.
CAUTION X Before the tilting movement move the axis so that it does not project from the
side moving downward.
X Carefully check the maximum possible axis tilt angle at the required axis
setting by increasing the tilt angle step by step; do not exceed this maximum
angle.
ATTENTION Do not operate the tilting table in case of an installed high-temperature dosing system
DS3241.
page 52 Assembling axes and tables
1. Turn the DSA100 aside. Four threaded holes are driven into the bottom.
3. Put the DSA100 onto the tilting table so that the rear panel connections of the
DSA100 and the tilting table lie on the same side.
4. Screw the four clamps delivered with the tilting table into the threaded holes to fix
the DSA100 at the table.
5. For save transport the tilting table is fixed with four fixing screws. Loosen these
screws (three turns counter clockwise) as described on the signs at the tilting table.
Assembling axes and tables page 53
6. The Emergency Stop button is pressed after delivery. Turn the button clockwise to
release it.
7. Connect the port on the rear side of the tilting table with one of the ports AXIS 1, 3
or 4 at the rear panel of the DSA100. Port AXIS 2 is reserved for the y-axis.
page 54 Single-Dosing units
8 Single-Dosing units
1. Take a syringe out of the packaging and pull out the plunger.
2. Remove the rubber attachment from the syringe plunger and place it on the metal
syringe plunger with setscrew.
3. Rotate the plunger upward and push it into the disposable syringe.
4. Rotate the syringe and slide its head under the holding clip.
Single-Dosing units page 55
1. Use the four hexagon socket screws provided to fasten the stand of the manual
dosing unit to the attachment plate.
Attachment
plate
* The setscrew on the right-hand side is used for adjusting the dosing unit to the
optical axis.
Syringe adaptor
3. Place the syringe holder with the two pins in the holes of the dosing stand and
fasten it to the stand with the screw.
page 56 Single-Dosing units
This chapter contains installation instructions for the Dosing unit DS3210 and the
belonging electronic module EM3200.
1
Plugs 1-2
Switch off and unplug the DSA100 before starting with the installation.
CAUTION
! Take care that the whole width of the socket is used for the board so that all poles
are occupied. If the board is not properly connected it can be destroyed when
switching on the DSA100.
2. Remove the covers from the illumination housing and the camera housing. In order
to do that, pull the spring inside the chamber and lift up the cover.
page 58 Single-Dosing units
3. Unscrew the upper cover from both sides (screws at the top of the left and right
chamber) and remove it.
4. The BUS board for plugging in the EM3200 board is located underneath the cover,
more precisely underneath the three white plastic frames (board support).
Loosen one screw on each of the frames and turn them aside.
5. From the rear view of the DSA100 the connections of the BUS board are labelled
upside down. Plug the EM3200 into two ports so that the electronic components
point to the front of the DSA100.
6. Connect the two plugs coming from the connection of the dosing unit...
...to the two ports 1 and 2 of the installed electronics board in an arbitrary order
(Ö ch. 8.2 ).
7. Return the supporting frames and fix them. Take care that the edge of the board
lies in the gaps of the frames.
1. Use the four hexagon socket screws provided to fasten the stand of the manual
dosing unit to the attachment plate.
Attachment plate
horizontal
alignment
* The setscrew on the right-hand side is used for adjusting the dosing unit to the
optical axis.
2. Connect the right-hand plug of the dosing carriage to the left-hand plug on the
upper side of the DSA100 housing.
Single-Dosing units page 61
3. Loosen the screw on the syringe unit slightly (do not remove it!). Insert the syringe
in the syringe adapter. To do this, press down the fastening for the syringe plunger
and insert the plunger.
4. When the syringe plunger has been inserted tighten up the screw again.
page 62 Single-Dosing units
5. Place the syringe unit on the dosing carriage. This is done by placing it over the
guide rail of the carriage and moving back the lever to fix the syringe unit in
position.
! Attention! The moment the DSA100 is switched off, the slide carrying the dosing
unit can move downwards because it is no longer kept in position by the motor.
If the syringe unit is fixed too low, the syringe can touch the sample and destroy
it.
Fix the syringe unit when the instrument is switched off. Take care that the
needle is in a small distance above the sample height required for
measurements. Also, readjust the height when using other syringes or
needles.
! Once the syringe unit is fixed, please still take care not to move the sample
table above the position required for measurements.
Single-Dosing units page 63
6. Connect the syringe unit to the remaining plug on the dosing carriage.
Some functions of the DSA software are restricted to multi-dosing units and cannot be
used for DS3210.
* Due to the simplified syringe control of the DS3210, no information about the
current syringe driver position is transferred to the software. Therefore, the filling
level display and the Auto-Refill function do not work for single-dosing.
! The valves and fittings of the dosing unit are not resistant against all liquids. Only
use liquids tested by KRÜSS which are listed on page 99 in the appendix. Please
contact KRÜSS if you want to use liquids that are not listed there.
* For installing a multi-dosing unit the DSA software must already be installed
(Ö DSA software manual).
Multi-dosing unit DS3226-3228 page 65
Depending on the number of syringes one or two syringe driver units with two to four
syringe drivers each must be assembled to left and right-hand chamber of the DSA100.
Overview
The following table shows the required combinations for all multi-dosing units.
Procedure
1. Open the compartment into which the syringe driver unit must be assembled.
page 66 Multi-dosing unit DS3226-3228
2. Remove the door of the compartment by pulling the lower spring inside the
compartment and lifting up the door.
3. Remove the covers from the illumination housing and the camera housing. In order
to do that, pull the spring inside the chamber and lift up the cover.
4. Unscrew the upper cover from both sides (screws at the top of the left and right
chamber) and remove it.
Multi-dosing unit DS3226-3228 page 67
5. Unscrew the covering plate that is fixed with two screws and remove it.
7. Lift the syringe driver unit to be assembled into the compartment and lead the
connecting cables through the gap that was previously covered.
! If two different syringe driver units are to be assembled please pay attention to
the correct assignment to the left and right-hand compartment (Ö page 72).
page 68 Multi-dosing unit DS3226-3228
8. Fix the syringe driver unit (in example picture: unit with two drivers) with the four
previously removed hexagon socket screws using the corresponding holes.
10. From the upper panel, eight tubes lead to the inside of the device. These tubes
serve as leads between syringes and dosing needle for the actual dosing tubes that
are assembled in a later step.
The following figure (rear view) shows the order of these tubes. Lead the tube no. 1
to syringe position 1 (to the compartment for the first syringes).
43 214 3 2 1
right left
2 1
chamber
Multi-dosing unit DS3226-3228 page 69
11. Thread the tube through the pierced rubber stopper so that about 0.5 cm jut out.
1 2
12. In the accessories case you find thin coloured tubes. Thread these tubes from the
upper side of the housing through the leading tubes until they reach the
compartments with the syringe driver units (the picture shows the front view).
1 2
12341234
right left
chamber
* For all further installation steps the colours of the accessories (fittings) should
suit the dosing tubes. It lends itself to use the colours as codes for the test
liquids used.
13. Take one fitting and one ferrule (see image) from the accessories case for each
dosing tube. Thread the free end of the tube in the compartment through a fitting
and put a ferrule on the tip of the tube.
Fitting Ferrule
page 70 Multi-dosing unit DS3226-3228
14. Screw off the protective caps from the valve blocks and screw on the fitting with
tube and ferrule.
! Screwing the fitting too tight can damage the ferrule and squeeze off the tube.
Do not use tools; the fitting should be easily unscrewed manually.
* Leave the DSA100 housing open in order to install the electronic module(s)
EM3200.
Multi-dosing unit DS3226-3228 page 71
Depending on the number of syringes belonging to the dosing unit, one, two or three
electronic modules EM3200 must be installed which control the syringe revolver and all
syringe drivers. This chapter contains an overview for the occupation and addressing
of the modules (Ö ch. 9.2.1 ) and instructions for assembling them (Ö ch. 9.2.2 ).
setting the
board’s address
1
2
Plugs 1-4
The first module to be plugged receives the address 4, the second and third the
addresses 5 and 6 in ascending order. For setting the address the slit of the screw
must point to the required address number.
Both the syringe revolver and the syringes drivers are connected to the electronic
module(s):
• The connections of the syringe revolver always occupy ports 1 and 2 of the first
electronic module (address 4).
• The syringe drivers occupy the further ports in a special order that depends on the
number of drivers. The plugs coming from the syringe drives are marked with 1 to 4
dots; the left syringe driver unit is assigned to syringe numbers 1-4, the right-hand
side unit to numbers 5-8.
page 72 Multi-dosing unit DS3226-3228
Overview
The following table contains an overview for the electronic modules EM3200 to be
installed for the different dosing units and their addresses and occupation.
Switch off and unplug the DSA100 before starting with the installation.
! Take care that the whole width of the socket is used for the board so that all poles
are occupied. If the board is not properly connected it can be destroyed when
switching on the DSA100.
1. The BUS board for plugging in the EM3200 board(s) is situated underneath the
cover, more precisely underneath the three white plastic frames (board support).
2. The frames serve to support the boards plugged in the BUS board. Loosen one
screw on each of the frames and turn them aside.
page 74 Multi-dosing unit DS3226-3228
3. Connect the two plugs coming from the connection of the dosing unit...
...to the two ports 1 and 2 of the first EM3200 board to be installed (address 4) in
an arbitrary order (Ö page 71).
4. Connect the other ports of all electronic modules as described on page 72. Pay
attention to the assignment of the plugs 1-4 and 5-8 coming from the syringe drives
to the ports 1-4 of the modules to be connected.
5. From the rear view of the DSA100 the connections of the BUS board are labelled
upside down. The slots of the BUS board can be occupied at will, the components
are recognized automatically.
6. Return the supporting frames and fix them. Take care that the edge of the board
lies in the gaps of the frames.
In order that all available syringes can be controlled an initialization file (.ini) matching
the dosing unit must be copied from the DSA-CD into the installation folder of the DSA
software on the computer.
5. Open the installation folder of the DSA version installed on your computer.
6. Paste the previously copied “DCM.ini” file from the clipboard into the installation
folder. Confirm that the existing file is to be overwritten.
page 76 Multi-dosing unit DS3226-3228
If syringes are already inserted and filled, liquid which escapes during the initialization
CAUTION can lead to a risk depending on the hazard class of the liquid
X Empty the syringes and lead the liquid into a waste container before starting
the initialization.
3. Select the tab sheet Dosing from the Device Control panel. Under the button on
the left side, a drop-down menu opens for selecting syringes.
4. Select the syringes one after another (except the one marked with M ) and carry out
the menu order Reset Position (DSA1) respectively Reset Syringe (DSA3) for
each syringe. The syringe drivers are moved into their start positions and initialized.
DSA1 DSA3
Multi-dosing unit DS3226-3228 page 77
2. Remove one of the syringes and the belonging allen wrench from the packaging.
3. Loosen the screw at the syringe plunger and remove the head.
5. Loosen the screw at the syringe adapter of the syringe drive using the allen
wrench.
page 78 Multi-dosing unit DS3226-3228
6. Push the syringe plunger into the syringe so that the length of the syringe with
plunger fits between the syringe driver and the valve block. Lead the plunger to the
stop into the syringe adapter and fix it with the hexagonal socket screw.
7. Push the syringe upwards and screw it into the luer-lock adapter.
Eight positions are located on the syringe revolver to which eight dosing needles are
assigned at maximum. In order to let the revolver find the correct position when a
syringe is selected, clamps and needles must be assembled to the revolver positions
correctly.
Letters A - H are assigned to the positions of the revolver. When the multi-dosing
system is delivered, position D of the syringe revolver is usually already occupied with
a clamp – this position is always used for the first syringe, regardless of how many
syringes are used for the dosing system.
H
A G
B F
C E
D=1
The occupation of all other positions depends on the number of syringes used.
page 80 Multi-dosing unit DS3226-3228
Overview
The following table shows the occupation of the syringe revolver for all multi-dosing
systems available.
3
DS3222 m DS3223
2 m 2
2 autom. 3 autom.
3 4
DS3224 m DS3225 5
4 2 m 3
4 autom. 5 autom.
4 5
DS3226 5 3 DS3227
6 4
m 7 3
6 autom. 7 autom.
m 2
1 manual (m) 6 2 1 manual (m) 1
1
5
DS3228 6 4
8 autom. 7 3
8 1 2
Multi-dosing unit DS3226-3228 page 81
1. Use four hexagon socket screws to screws the syringe revolver to the attachment
plate for the dosing unit. For some dosing systems, manual screws are provided
instead.
attachment
plate
2. Screw the clamps with two screws each onto the required positions of the syringe
revolver. An extended clamp is to be used for the manual syringe.
clamp for
manual syringe
3. Assemble the needle carriers onto the free tubes for the test liquid. You require a
needle adapter, a metal shell with a small ring, a fitting and a ferrule from the
accessories case.
! The metal rings are already located in the shells, please take care not to loose
them.
* The thin needles are intended for sessile drops, the thicker ones for pendant
drops (surface tension measurements).
5. Click the needle carriers into the clamps of the syringe revolver. Make sure that the
bottles and liquids are correctly assigned to the syringe positions (image shows
front view).
1 2 3 4 5 6 7 8
6. Connect the syringe revolver to the left-hand connector on the upper side of the
DSA100 housing.
Multi-dosing unit DS3226-3228 page 83
1. Put the rack for the test liquid bottles into the chamber(s).
2. Lead the tube from the syringes through the cap inserts and caps of the bottles.
3. Puncture the silicone seal with one of the needles supplied. Leave the needle in the
hole.
4. Fill the storage bottles with the test liquids. Make sure that the bottles and liquids
are correctly assigned to the positions (max. 1-8). Label the bottles.
5. Place the cap inserts in the caps and screw the caps onto the storage bottles.
Place the storage bottles in the recesses provided for them in the base of the
compartment.
* The storage bottles delivered by KRÜSS stick to the bottom of the rack
magnetically.
page 84 Multi-dosing unit DS3226-3228
In a further step the dosing unit must be adjusted with respect to the camera so that
the middle horizontal needle position is also in the middle of the recorded image.
1. Start the DSA software. Either the live image or a stationary image can be seen in
the frame grabber window. The camera symbol in the symbol bar must be shown
as being pressed ; if this is not the case please click on the icon.
2. Select the tab sheet Dosing from the Device Control panel.
DSA1 DSA3
Click on the button at the top left. Select Syringe 1 (S1 ) in the drop-down menu.
* If a different syringe had been selected previously the syringe selector will
rotate to Position 1.
3. Select the tab sheet Needle Pos (DSA1) respectively N-Pos (DSA3).
DSA1 DSA3
4. Move the horizontal slide controller to the middle position. The displayed value
must be set to zero.
5. Use the vertical slide controller (or the <↓> key) to move the syringe to a position
between the camera and the light source.
Multi-dosing unit DS3226-3228 page 85
6. Set the illumination, zoom and focus for the needle image. For software-controlled
optics, this is carried out in the tab sheet Imaging (DSA1) respectively O-Con
(DSA3).
7. Use the setscrew on the right-hand side of the dosing unit to bring the needle in the
middle position in the video image.
8. Zoom the needle image and repeat the procedure in order to set the middle
position as accurately as possible.
page 86 Environmental chambers
10 Environmental chambers
Several environmental chambers are available for the DSA100 which allow
temperature- or humidity-controlled measurements. This chapter contains the
necessary installation instructions.
Thermostatted Chambers TC30 and TC40 are used for drop shape analysis above and
below room temperature.
! In order to avoid soiling the viewing window, do not touch it with your fingers. Avoid
contacting the window with sharp objects.
• TC40: -30°C – 160°C* by using a Peltier element. A liquid thermostat must addi-
tionally be connected for counter-cooling.
! Do not set the temperature to values outside the given range. Otherwise the
chamber could be damaged.
Silicone oil must not be used as a thermostat liquid because it attacks the seals
and tubes. Vegetable oils and ethylene glycol are suitable liquids.
2. Move the sample table downward. Remove the sample table adapter.
10.1.2 Connections
Dosing inlet
Temperature sensor
inlet
Condensation protec-
tion for thermal con- Inlet for protective gas
ductivity hood window or HC10 sensor
Condensation protec-
tion for outer window
channels for
thermostat liquid
page 88 Environmental chambers
Thermostat connection
Make sure that the instrument is switched off. Plug the mains cable into the socket
on the rear panel of the control unit. Connect the control unit to the mains supply.
Connect the cable from the TC40 to the socket on the front panel of the control unit.
This is done by turning the plug until the suitable position has been reached and
then turning the ring clockwise until the plug clicks into position.
Condensation protection
At a high relative humidity condensation could form on the viewing and measuring
window with measurements below room temperature. In order to prevent this, the
assembled tube connections are used to direct a stream of air through the chamber
and past the windows.
Measurements with thermal conductivity hood: Connect the upper and lower
connections to a supply of compressed air (approx. 500 mbar).
Environmental chambers page 89
A connection is provided in the centre of the rear panel of the chamber for working
under a protective gas. If condensation protection is also necessary then additionally
connect the protective gas to the connections provided for it. In both cases do not set
too high a value for the gas flow, as this could result in temperature variations.
The temperature above the sample can be measured with an external PT100
temperature sensor.
* If the thermal conductivity hood is used then this must be placed in position before
the temperature sensor is inserted.
With the drawer closed slide the sensor into the guide provided for it on the top of
the chamber.
Insert the plug of the PT100 into the socket provided for it on the illumination unit of
the DSA100 (Ö ch. 5.11).
! Make sure that the inserted samples do not come into contact with the sensor; if
necessary pull the sensor out slightly.
page 90 Environmental chambers
* The optionally used thermal conductivity hood is made from a highly thermally
conductive material and ensures optimal thermal transport in the interior of the
chamber. It should be used when there is a great difference to the outside
temperature and whenever temperature variations are to be minimized. However, if
the thermal conductivity hood is used the target temperature will be achieved more
slowly. If you are more concerned with speed than accuracy of the temperature
regulation then you can dispense with the hood.
1. Pull out the drawer, remove the two holding clips and position the sample on the
plate.
5. If necessary insert the PT100 temperature sensor PT100 and fix it at a height just
above the sample.
Environmental chambers page 91
10.1.4 TC40 only: setting and reading off the measuring temperature
This chapter covers only the Peltier chamber TC40; with the TC30 the temperature is
controlled by the connected thermostat.
X Always switch the thermostat on before the control unit and set a suitable
CAUTION temperature (Ö p. 92).
X Make sure that the flow is not impeded by kinks in the tube.
Immediately after the mains switch is switched on the control unit initializes itself and is
ready for use after about 3 seconds. The required temperature is set by using the
“CONTROLLER” on the control unit of the TC40.
The set temperature is shown in the lower line of the display. The upper line shows the
actual temperature of the heating element (not the temperature measured at the
external temperature sensor).
The set temperature is set by using the two arrow keys ( = temperature increase;
= temperature decrease). Short presses alter the temperature in 0.1°C steps;
longer presses change the setting rapidly.
* The two keys and have no function for TC40; if they are pressed only the
temperature unit °C will be shown.
page 92 Environmental chambers
With the TC40 the thermostat is used to mediate between room temperature and target
temperature.
! The given temperature limits of 160°C or –30°C can only be achieved when the
Peltier element is supported by the thermostat.
The following Table assumes that water is used as the thermostat liquid:
The Humidity Chamber HC10 is used together with thermostatted Chambers TC30 or
TC40.
! If a TC40 is used then not the Peltier element is used for thermostatting. Instead
the temperature control of both thermostatted chambers is carried out by an
external thermostat.
! When measuring with TC40 with the HC10 chamber connected, a temperature of
120°C must not be exceeded.
If Humidity Chamber HC10 is not acquired together with the DSA100, but is delivered
subsequently, then an interface board and the connection socket for the HC10 must
additionally be built into the DSA100.
This is done as described in ch. 7.1 for the axes, but with two differences:
• The connection socket for the chamber is built into the left-hand side of the rear
panel of the DSA100.
• The connection plate is also built in on the left. The right-hand side is reserved for
the axes.
page 94 Environmental chambers
1. Screw off the guide for the temperature sensor from the top of the chamber.
2. Screw out the two upper screws at the back on both the left-hand and right-hand
chamber sides and the two screws on the top as shown in the illustration.
3. Screw out the large screw on the rear panel (or the protective gas connection, if
present).
4. Screw the two screws used to hold the temperature sensor guide into their
openings for a few turns.
5. Slide the HC10 Chamber over the Thermostatted Chamber from the rear. Lead the
tube for the condensation protection through the opening in the side.
Environmental chambers page 95
Push the chamber until it reaches a stop against the two screws on the top.
6. Fasten the HC10 Chamber on the thermostatted chamber with two screws each on
the sides and top.
! The flow control valve reduces the pressure of the gas to a constant value of
350 mbar. This is permanently set and locked at this value. Do not try to adjust
the valve.
3. Optional: connect the gas flow for condensation protection to a special compressed
connection (approx. 500 mbar).
page 96 Environmental chambers
* The following illustration shows all the gas and liquid connections for operating the
humidity chamber.
gas stream: condensation gas stream: humidity regulation (2 bar
prevention initially, reduced to 350 mbar by a valve)
(optional; 500 mbar)
Thermostate
! The gas connection on Chambers TC30/40 should only be used when the
observation window is actually affected by condensation. The additional flow of air
reduces the atmospheric humidity that can be achieved and reduces the accuracy
of the humidity control.
! If a low humidity in the air is required then dried gas must be used (e.g. dried in a
silica gel air drier). If the flow of gas is produced by a compressor then the air must
be dried if the humidity of the air used for the measurement is to be lower than the
atmospheric humidity.
! Oil-free gas must be used for all gas connections (air filter!).
Connect the multi-pin connection on the rear panel of the HC10 to the
corresponding connection on the rear of the DSA100 frame (left-hand connector
plate, rear view).
Environmental chambers page 97
* We recommend using the thermal conductivity hood for measurements with the
HC10. This reduces variations in temperature and humidity.
Screw off the plate on the rear panel of the thermal conductivity hood
(Torx 9 wrench).
Screw out the screw marked in the illustration. Fill the chamber to the brim with
demineralized water.
! Depending upon the set humidity the water will evaporate at different rates. When
the chamber is less than one-third full with water its performance falls off rapidly.
During operation you should check the filling level about once every three hours
and top up with water as necessary
! If you are working with an inclined table then the screw must be screwed in after
filling. If the standard axes are used it can be left unscrewed.
page 98 Environmental chambers
* As the sensor holder has been removed from the Thermostatted Chamber in order
to attach the HC10, the temperature sensor is inserted in the free opening.
Pierce one of the silicone seals provided, push the sensor through the opening and
insert the temperature sensor in the opening of the Thermostatted Chamber so the
silicone seal lies on the opening.
Insert the plug of the PT100 into the socket provided for it on the illumination unit of
the DSA100 (Ö ch. 5.11).
page 99 Appendix
Appendix
The following liquids were tested with the multi-dosing unit DS3222-3228 and can be
used as standard test liquids.
• Water
• Diiodomethane
• Ethylene glycol
• α-bromo-naphthalene
• Glycerol
• n-Hexadecane
• Benzyl alcohol
! Please contact KRÜSS if you want to use liquids that are not listed there.
Materials of the dosing unit that come into contact with the test liquid
! Do not use test liquids to which the materials listed in the table below are not
resistant:
Component Material:
Tubes PTFE
Should technical problems with the DSA100 or the DSA-Software occur please contact
our technical support service or your local dealer:
KRÜSS GmbH
Borsteler Chaussee 85 – 99a
22453 Hamburg Germany
E-mail: [email protected]
Tel.: +49/040/514401-0
Fax: +49/040/514401-98
Please also visit our webpage on the Internet:
www.kruss.de
The warranty terms and deadlines are described in the general terms and conditions of
KRÜSS GmbH.
There is no warranty claim if the device was damaged due to careless usage or
improper treatment or if it was not used according to purpose, and if the instructions
and danger warnings in the manual were not observed.
The warranty claim is void if changes on the device are performed by the customer or a
third party without prior consultation of KRÜSS GmbH, which go beyond the activities
described in the manual. This also applies to repair work, which was independently
performed or carried out by a third party.
The information contained in this document may be altered without notice and does not
represent any obligation by KRÜSS GmbH.
Neither this manual nor any part of it may be reproduced or transmitted for any
purpose whatsoever in any form and by any means, electronic or mechanical, by
photocopy or drawing without the express approval of KRÜSS GmbH in writing.