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    18551507-079 (BS V (B) )

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    SHOAIB NAVEED
    This document describes a procedure for determining the amount of glucose in a sample using Fehling's solution. Key steps include: 1) Preparing a standard glucose solution of known concentration for titration. 2) Titrating aliquots of Fehling's solution with the standard glucose solution to determine the volume needed to reduce the copper. 3) Titrating the sample glucose solution in the same way and calculating the concentration based on the volume used and concentration of the standard.

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    18551507-079 (BS V (B) )

    Uploaded by

    SHOAIB NAVEED
    379 views3 pages
    This document describes a procedure for determining the amount of glucose in a sample using Fehling's solution. Key steps include: 1) Preparing a standard glucose solution of known concentration for titration. 2) Titrating aliquots of Fehling's solution with the standard glucose solution to determine the volume needed to reduce the copper. 3) Titrating the sample glucose solution in the same way and calculating the concentration based on the volume used and concentration of the standard.

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    18551507-079(BS V (B))

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    This document describes a procedure for determining the amount of glucose in a sample using Fehling's solution. Key steps include: 1) Preparing a standard glucose solution of known concentration for titration. 2) Titrating aliquots of Fehling's solution with the standard glucose solution to determine the volume needed to reduce the copper. 3) Titrating the sample glucose solution in the same way and calculating the concentration based on the volume used and concentration of the standard.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    379 views3 pages

    18551507-079 (BS V (B) )

    Uploaded by

    SHOAIB NAVEED
    This document describes a procedure for determining the amount of glucose in a sample using Fehling's solution. Key steps include: 1) Preparing a standard glucose solution of known concentration for titration. 2) Titrating aliquots of Fehling's solution with the standard glucose solution to determine the volume needed to reduce the copper. 3) Titrating the sample glucose solution in the same way and calculating the concentration based on the volume used and concentration of the standard.

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    © All Rights Reserved
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    of 3

    DETERMINATION OF GLUCOSE WITH THE HELP OF FEHLING'S

    SOLUTION
    Principle:

    Glucose also reduces Fehling's solution quickly, the latter is obtained by mixing an aqueous solution of
    copper sulphate with alkaline solution of sodium potassium tartrate (Rochelle salt). The latter prevents the
    precipitation of cupric hydroxide by forming a complex. A freshly prepared Fehling's solution is first
    standardized by titration against a standard glucose solution. The standardized Fehling's solution is then
    used to determine the amount of glucose in an unknown sample.

    Reactions:

    Apparatus: Chemicals

    Burette (50 cm3) Glucose

    Pipette (10 cm3) Copper sulphate

    Conical flask (250 cm3) Sodium hydroxide

    I Vol. flasks (250 cm3) Sodium potassium

    Beaker (250 cm3) tartrate

    Weighing bottle Methylene blue

    Funnel (small)

    distilled water

    Burette stand
    Procedure:

    Preparation of Standard solution of glucose

    Dissolve accurately weighed quantity of glucose about 1.25 g in distilled water and make up to 250 cm3
    in a volumetric flask.

    Titration with standard glucose solution: Fill the burette with standard glucose solution. Pipette out 10
    cm3 each of Fehling's solution A and B in a 250 cm3 conical flask and dilute it with 25 cm3 water. Boil
    the solution gently over a wire gauze and titrate with standard glucose solution, (taken in burette)
    adding 1 cm3 of the solution at a time till the blue color of the solution just disappears and red
    precipitate of Cu20 begins to appear. Continue the heating of the Fehling's solution during the titration.
    Repeat the process to get at least two concordant readings.

    Titration with unknown glucose solution: Now take unknown glucose solution in burette and repeat the
    titration exactly in the sh.ne manner as described for standard glucose solution. To detect the end point
    more accurately add 4-5 drops of methylene blue indicator to the Fehling's solution just before the end
    point. The disappearance of the color will give exact and point. Record the Observations.

    Observations & Calculations:

    Suppose 10 cm3 of standard glucose solution are used to reduce completely = 10 cm3 of Fehling’s
    solution.

    250 cm3 of standard glucose solution contains = 1.25 g of glucose

    1 cm3 of standard glucose solution contains = 1.25 250 = 0.005 g of glucose

    10 cm3 of standard glucose solution contains = 0.005 x 10 = 0.05 g of glucose

    As 10 cm3 of standard glucose solution reduces = 10 cm3 of Fehling’s solution.

    So, 0.05 g of glucose reduces = 10 cm3 of Fehling’s solution.

    12 cm3 of given glucose sample solution = 10 cm3 of Fehling’s solution.

    But 10 cm3 of Fehling’s solution = 0.05 g of glucose

    So, 12 cm3 of = 0.05 g of glucose

    100 cm3 of given glucose sample solution = 0.05 12 x 100 = 0.416 g of glucose

    Results:

    The given glucose sample solution contains 0.416 g of glucose.

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