Scribd Logo
Upload

Welcome to Scribd!

  • 58 views

    Leakiness: Lab 2: Neural Signalling

    Uploaded by

    Afroza Shoily
    This lab activity reinforces concepts about neural signalling introduced in the textbook. It involves calculations to determine electrochemical equilibrium potentials and resting membrane potentials based on varying ion concentrations and membrane permeabilities. Key concepts covered include the definitions of ion channels and active transporters, how leakage prevents passive conduction in axons requiring active conduction, and the relationships between ion concentrations, membrane permeabilities, and resulting membrane potentials.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Leakiness: Lab 2: Neural Signalling

    Uploaded by

    Afroza Shoily
    58 views2 pages
    This lab activity reinforces concepts about neural signalling introduced in the textbook. It involves calculations to determine electrochemical equilibrium potentials and resting membrane potentials based on varying ion concentrations and membrane permeabilities. Key concepts covered include the definitions of ion channels and active transporters, how leakage prevents passive conduction in axons requiring active conduction, and the relationships between ion concentrations, membrane permeabilities, and resulting membrane potentials.

    Original Title

    Week 3 lab

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    This lab activity reinforces concepts about neural signalling introduced in the textbook. It involves calculations to determine electrochemical equilibrium potentials and resting membrane potentials based on varying ion concentrations and membrane permeabilities. Key concepts covered include the definitions of ion channels and active transporters, how leakage prevents passive conduction in axons requiring active conduction, and the relationships between ion concentrations, membrane permeabilities, and resulting membrane potentials.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    58 views2 pages

    Leakiness: Lab 2: Neural Signalling

    Uploaded by

    Afroza Shoily
    This lab activity reinforces concepts about neural signalling introduced in the textbook. It involves calculations to determine electrochemical equilibrium potentials and resting membrane potentials based on varying ion concentrations and membrane permeabilities. Key concepts covered include the definitions of ion channels and active transporters, how leakage prevents passive conduction in axons requiring active conduction, and the relationships between ion concentrations, membrane permeabilities, and resulting membrane potentials.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 2

    Name: Afroza Parvin

    Student ID: 45478503

    Introduction to Neuroscience 1 (COGS1000)


    Lab 2: Neural Signalling
    Activity Sheet

    This lab activity covers information and reinforces concepts introduced in Chapter
    2 (and specifically pp. 33-46)

    1. Define ion channels and active transporters. What is the relationship between
    them?

    Ion channels: The channels that permits the movement of particular ions while excluding ions and ions can diffuse across the membrane. Active transporters: A transporter by which ions can move against the concentration gradient.

    2. What property of axonal membranes prevents effective passive conduction of electrical


    signals and make active conduction necessary?

    leakiness
    3. Describe the difference between passive current flow and active current flow in an
    axon.

    PCF : Current leaks out across membrane ACF: Current sustained over long distance
    4. Define electrochemical equilibrium

    potential change across the membrane for the net in flux of K+

    5. Electrochemical Equilibrium Potential.


    A. Which equation is used to calculate the equilibrium potential? Nearst equation

    B. Vary the concentrations of K+ and Na+, both inside and outside the cell. What
    effect does this have on the electrochemical equilibrium potential of the ion?

    The membrane is much more permeable to K+ than to Na+, so the resting potential is closer to the equilibrium potential of K+.

    C. What is the Na+ equilibrium potential when Na+ out equals 100 mM and Na+ in
    equals 10 mM?
    58
    D. What is the Na+ equilibrium potential when Na+ out equals 100 mM and Na+ in
    equals 100 mM?
    0
    E. What is the K+ equilibrium potential when K+ out equals 10 mM and K+ in equals
    100 mM?
    -58
    F. Include some reflections on the pattern of results you observed in A-D.

    The Na+ rushes back which makes the inside of the membrane more positive and outside becomes negative

    6. Resting Membrane Potential.


    A. When you calculate the membrane potential when more than one ion species is
    involved or permeabilities may vary, which equation is used? Goldman equation
    B. Starting with the default parameter values, vary the relative membrane permeability
    to K+ and Na+. What effect does this have on the resting membrane potential?
    When K+ decreases, the membrane potential increase, when K+ increases the membrane potential decreases; When Na+ decreases the membrane potential decrease, when Na+ increases the membrane potential increase

    C. Set Na+ out to 100 mM, Na+ in to 10 mM, K+ out to 10 mM and K+ in to 100 mM.
    What is the membrane potential when the Na+ permeability equals 0 and the K+
    permeability equals 10? -58
    D. What is the membrane potential when the Na+ permeability equals 10 and the K+
    permeability equals 0? 58
    E. What is the membrane potential when the Na+ permeability equals 1 and the K+
    permeability equals 10?
    -40.79
    F. Why is this?

    The movement of K+ makes it positive and for Na+ makes it negative.

    You might also like