Leica DMi8

Instructions for Use t Gebrauchsanweisung t Mode d’emploi

Leica Microsystems CMS GmbH t Instructions 11934056, Revision 1.1 from 2015-07-15
COPYRIGHT/ CONTENTS

2
Leica DMi8
Instructions for Use
Leica Microsystems CMS GmbH t Instructions 11934056, Revision 1.1 from 2015-07-15
COPYRIGHT/ CONTENTS

Copyright Contents
All rights to this documentation are held by Leica Micro- 1 Important Notes about this Manual ......................... 4
systems CMS GmbH. Reproduction of text or illustrations 1.1 Text symbols and their meanings .............................. 4
(in whole or in part) by print, photocopy, microfilm or other
method (including electronic systems) is not allowed with- 2. Intended Purpose of the Microscope ...................... 6
out express written permission from Leica Microsystems 3. Safety Notes ................................................................. 8
CMS GmbH. 3.1 General safety notes ................................................... 8
3.2 Electrical safety ........................................................... 8
The instructions contained in the following documentation 3.3 Laser safety ................................................................ 11
reflect state-of-the-art technology. We have compiled the 3.3.1 Safety switch (interlock) on the microscope ........ 11
texts and illustrations as accurately as possible. Still, we 3.3.2 Screwed-on filter turret cover................................. 12
are always grateful for comments and suggestions regard- 3.3.3 Laser coupling by the user ....................................... 12
ing potential mistakes within this documentation. 3.3.3.1 Safety hazard due to folded back
Transmitted Light arm ............................................... 13
The information included in this manual may be changed with- 3.3.3.2 Safety hazard at eyepiece........................................ 13
out prior notice. 3.3.3.3 Safety hazard in the condenser area
during operation. ....................................................... 13
3.3.3.4 Safety hazard above the condenser....................... 14
3.3.3.5 Safety hazard in the specimen area ....................... 14
3.3.3.6 Safety hazard due to openings at microscope ..... 15
3.3.3.7 Note regarding the safety hazards in Sections
3.3.3.1 and Section 3.3.3.2 ......................................... 16
3.3.4. Exception for Class 1 lasers ..................................... 17
3.5 Storage and transport ............................................... 18
3.6 Ambient conditions.................................................... 18
3.7 Notes on handling light sources ............................. 19
3.8 Notes on handling the Touch Screen ..................... 20
3.9 Notes on handling immersion oil............................. 20
3.10 Notes on handling acids and bases ....................... 20
3.11 Disposal ....................................................................... 20
4. Leica DMi8 configurations instrument overview 21
4.1 Microscope configurations (examples) ................. 21
4.2 Dimensions (specified in mm).................................. 22
4.3 Specifications+) .......................................................... 24
4.4 Controls ....................................................................... 28
4.4.1 Control panel fields.................................................... 29
4.4.2 Additional control elements ..................................... 31
4.4.3 The Touch Screen ...................................................... 34
4.4.4 The Leica SmartMove control element.................. 36
Revision 1.1, published on 2015-07-15 by: 4.4.5 Leica STP4000/8000 external control panel........... 37

Leica Microsystems CMS GmbH

Ernst-Leitz-Straße 17-37
D-35578 Wetzlar (Germany)
http://www.leica-microsystems.com
Responsible for contents:
Marketing CMS
2
CONTENTS

5. Operation .................................................................... 38 6. Troubleshooting ......................................................... 86

5.1 Switching on the microscope .................................. 39
5.2 Illumination ................................................................. 41 7. Care of the Microscope............................................ 89
5.2.1 Transmitted Light........................................................ 41 7.1 Dust Cover ................................................................... 89
5.2.2 Fluorescence .............................................................. 42 7.2 Cleaning ....................................................................... 89
5.2.3 Infinity port .................................................................. 44 7.3 Handling acids and bases ........................................ 90
5.2.4 Shutter ......................................................................... 45 8. Essential Consumable and Spare Parts ............... 91
5.3 Objectives ................................................................... 46
5.4 Tube .............................................................................. 49 9. Appendix .................................................................... 92
5.5 Eyepieces .................................................................... 50 9.1 Assigning the variable function keys ..................... 92
5.6 Stages and object displacement............................. 51 9.2 Abbreviation glossary ............................................... 94
5.6.1 Object displacement ................................................. 51 9.3 Overview of Touch Screen pictograms .................. 95
5.6.2 Adjusting the step sizes on motorized stages....... 52 9.4 Additional information............................................... 96
5.7 Focusing ...................................................................... 54
10. Index ............................................................................ 97
5.7.1 Focusing the image ................................................... 54
5.7.2 Focus thresholds ........................................................ 55 11. EC Declaration of Conformity .................................. 99
5.7.3 Adjusting the step sizes ............................................ 56
5.7.4 AFC (Adaptive Focus Control) .................................. 57
5.8 Selecting the ports .................................................... 58
5.9 Magnification Changer ............................................. 59
5.10 Aperture diaphragm and field diaphragm ............. 60
5.11 Filter ............................................................................. 62
5.11.1 Transmitted Light filter .............................................. 62
5.11.2 Fluorescence Cubes.................................................. 63
5.11.3 Leica RFID Module......................................................... 65
5.11.3.1 Product Description ................................................. 65
5.11.3.2 Specifications LID Module 1.0 ...............................65
5.11.3.3 Specifications RFID Tag D6.7 special ..................... 65
5.11.3.4 Ports ............................................................................. 65
5.11.3.5 FCC / IC Compliance .................................................. 65
5.12 The memory function on the Touch Screen .......... 66
5.13 Transmitted Light contrast method ......................... 66
5.13.1 Brightfield (TL) ............................................................ 67
5.13.2 Phase Contrast (TL) ................................................... 69
5.13.3 Darkfield (TL) .............................................................. 71
5.13.4 Polarization (TL) ......................................................... 72
5.13.5 Differential Interference Contrast – DIC (TL) ....... 74
5.13.6 Integrated Phase Contrast – IPH (TL) .................... 76
5.13.7 Integrated Modulation Contrast – IMC (TL) .......... 78
5.14 Fluorescence .............................................................. 82
5.15 Combination method ............................................... 85

3
1. IMPORTANT NOTES ABOUT THIS MANUAL

1 Important Notes about this Manual

Caution!
These Instructions for Use are an essential component
of the product; they must be read carefully before the
product is assembled, put into operation or used, and
must be kept for later reference.

These Instructions for Use include important instructions

and information related to the operating safety and mainte-
nance of the instrument.

1.1 Text symbols and their meanings

(1.2) Numbers in brackets, e.g. (1.2), refer to illustrations, in the

example to Fig. 1, item 2.

o p.20 Numbers with an arrow, e.g. o p. 20, refer to a specific

page of the manual.

WARNING indicates a hazard with a medium degree of risk

WARNING that, if not avoided, can result in death or serious injury.

CAUTION indicates a hazard with a low degree of risk that,

CAUTION if not avoided, can result in minor or moderate injury.

Caution!
In this manual, additional safety notes are indicated with
the triangle symbol shown here, and have a gray back-
ground.

Caution! The instrument and accessories can be damaged

! when operated incorrectly.

4
1. IMPORTANT NOTES ABOUT THIS MANUAL

Warning of hazardous electrical voltage! Risk of electrical

shock!

Danger due to hot surface

Warning of optical radiation! Never look directly into the

light beam! Wear safety goggles!

Warning of electromagnetic field

Warning of UV radiation! Wear safety goggles!

Warning of hazardous laser radiation – take appropriate

safety precautions!

Warning of hand injuries

Instructions for disposing of the instrument, its acces-

sories and consumables.

Connection to ground

Explanatory note

Item not contained in all equipment configurations

*

MM/YYYY
Manufacturing date, e.g. 11 / 2011 for November 2011

China RoHS 50 years EFUP

(Environmentally friendly use period)

5
2. INTENDED PURPOSE OF THE MICROSCOPE

2. Intended Purpose of the Microscope

DMi8 Leica microscopes are inverted light microscopes Directives of European Community (EC-Directives)
and are intended for use as general lab microscopes for The named microscope meets the requirements of the
routine examinations of biological specimens. Council Directives 2006/95/EC and 2014/35/EC concerning
electrical apparatus and 2004/108/ EC and 2014/30/EC con-
The Leica DMi8 series can be used universally. All con- cerning electromagnetic compatibility.
trast methods, such as Brightfield, Darkfield, Phase
Contrast, DIC, fluorescence or modulation contrast,
are integral components of the microscope and can
be adapted or exchanged quickly and easily. Vari- Reasonably foreseeable misuse
able illumination and imaging beam paths as well as
The following are prohibited:
HCS optics, modular accessories and a wide range of available
t
To use the microscope for any purpose not in accordance
peripherals supplement the inverted Leica research stand
with the Declaration of Conformity (e.g. the use of medical
from Leica Microsystems CMS GmbH.
products in accordance with EU Directive 93/42/EEC)
t
Making use of auxiliary equipment to use the area be-
Leica DMi8 microscopes:
tween the microscope stage and objectives as a clamp or
Leica DMi8 manual 11889110 holder (working like a vice)
Leica DMi8 coded 11889111 t
Operating the microscope in an inclined position
Leica DMi8 automated 11889112 t
Cleaning the microscope in a way other than specified in
Leica DMi8 automated 11889113 the manual
Leica DMi8 automated 11889014 t
Modifying the built-in safety circuits in the instrument
t
Allowing unauthorized personnel to open the instrument
t
Using cables that Leica Microsystems CMS GmbH has not
provided or permitted
t
Using the swiveling Touch Screen of the microscope as a
transport handle
t
Using combinations with non-Leica components that go
beyond the scope of the manual

6
2. INTENDED PURPOSE OF THE MICROSCOPE

Caution!
WARNING
The manufacturer assumes no liability for damage Notes on handling laser devices
caused by, or any risks arising from, using the micro- In the standard design without additional laser safety
scope for other purposes than those for which they are precautions, the microscopes are not suitable for cou-
intended or not using them within the specifications of pling laser radiation (such as to the camera ports), since
Leica Microsystems CMS GmbH. this radiation poses a hazard for the user (eye injury in
In such cases, the Declaration of Conformity shall be in- particular).
valid. For use of the microscope with lasers,
Leica Microsystems CMS GmbH offers special micro-
scope variants with additional safety features. Laser
couplings require corresponding safety devices that
have to be inspected and installed by trained personnel.
For further information, please contact your authorized
Leica Microsystems CMS GmbH representative.

7
3. SAFETY NOTES

3. Safety Notes
3.1 General safety notes
These instruments of protection class 1 are built and in- Caution!
spected in accordance with
EN 61010-1 /IEC 61010-1 Safety requirements for The instruments and accessories described in this In-
electrical equipment for measurement, control and labora- structions for Use have been tested for safety and po-
tory. tential hazards.
The responsible Leica Microsystems CMS GmbH af-
They also meet the requirements in filiate or the main plant in Wetzlar must be consulted
EN / IEC 61326-1 Electrical equipment whenever the device is altered, modified or used in con-
for measurement, control and laboratory use – EMC re- junction with non-Leica components that are outside of
quirements. the scope of this manual.

The products were also tested in accordance with EN Unauthorized alterations to the instrument or noncom-
62471 / IEC 62471 Photobiological safety of lamps and lamp pliant use shall void all rights to any warranty claims!
systems.
3.2 Electrical safety
General specifications
Caution! Compact Leica CTR electronics box
In order to maintain this condition and to ensure safe For indoor use only.
operation, the user must follow the instructions and Supply voltage: 100 – 240 VAC
warnings contained in this operating manual. Frequency: 50 / 60 Hz
Power consumption: max. 150 VA
Fuses: 3.15 A, slow-blowing,
Breaking capacity H, 250 VAC
Size: 5x20 mm
Ambient temperature: 15° - 35°C
Relative humidity: 90% up to 30°C,
non-condensing
Protection class: I
Overvoltage category: II
Pollution degree: 2

8
3. SAFETY NOTES

Advanced/Advanced+ Leica CTR electronics box Leica DMi8 automated

For indoor use only. For indoor use only.
Supply voltage: 100 – 240 VAC Supply voltage: 100 – 240 VAC (oLeica CTR)
Frequency: 50 / 60 Hz Frequency: 50 / 60 Hz (oLeica CTR)
Power consumption: max. 290 VA Power consumption: see Leica CTR
Fuses: 6.3 A, slow-blowing, Fuses: see Leica CTR
Breaking capacity H, 250 VAC Ambient temperature: 15° - 35°C
Size: 5x20 mm Relative humidity: 90% up to 30°C,
Ambient temperature: 15° - 35°C non-condensing
Relative humidity: 90% up to 30°C, Protection class: I (oLeica CTR)
non-condensing Overvoltage category: II (oLeica CTR)
Protection class: I Pollution degree: 2 (oLeica CTR)
Overvoltage category: II
Pollution degree: 2
Leica EL6000*
For indoor use only.
Leica DMi8 manual
Supply voltage: 100 – 240 VAC
For indoor use only. Frequency: 50 / 60 Hz
Supply voltage: 100 – 240 VAC Power consumption: max. 200 VA
Frequency: 50 / 60 Hz Fuses: 2.5 A, slow-blowing,
Power consumption: max. 55 VA Breaking capacity H, 250 VAC
Fuses: 1.6 A, slow-blowing, Size: 5x20 mm
Breaking capacity H, 250 VAC Ambient temperature: 0° - 40°C
Size: 5x20 mm Relative humidity: 90% up to 30°C,
Ambient temperature: 15° - 35°C non-condensing
Relative humidity: 90% up to 30°C, Protection class: I
non-condensing Overvoltage category: II
Protection class: I Pollution degree: 2
Overvoltage category: II (* See provided Instructions for Use)
Pollution degree: 2

Leica DMi8 coded

For indoor use only.
Supply voltage: 100 – 240 VAC
WARNING
Frequency: 50 / 60 Hz
The power plug may only be plugged into an outlet
Power consumption: max. 55 VA
equipped with a grounding contact.
Fuses: 1.6 A, slow-blowing,
Breaking capacity H, 250 VAC
Do not interfere with the grounding function by using an
Size: 5x20 mm
extension cord without a ground wire. Any interruption
Ambient temperature: 15° - 35°C
of the ground wire inside or outside of the instrument, or
Relative humidity: 90% up to 30°C,
release of the protective conductor terminal, can cause
non-condensing
the instrument to become hazardous. Intentional ground
Protection class: I
interruption is not permitted!
Overvoltage category: II
Pollution degree: 2

9
3. SAFETY NOTES

Caution!
! Caution!
Only insert or unplug data and control circuits when the
By connecting it to the grounding connection (earth
instrument is switched off; otherwise, the instrument
screw at the back of the electronics box) ancillary
may be damaged.
equipment with its own and/or extra power supply may
be brought to the same ground wire potential. For con-
nections without a ground protection conductor, Leica
Microsystems CMS GmbH Service must be consulted.
WARNING
By definition, the main circuit breaker of this instrument
is the connection between the power cable and device
Caution! port. The user must ensure unobstructed access to the
main circuit breaker at all times.
Never use any fuses as replacements other than those
of the types and the current ratings listed here. Using
other fuses or bridging the fuse holder is not permitted.
The use of incorrect fuses may result in a fire hazard.
WARNING
Only use power cables, or alternative cables with VDE/
HAR codes that minimally fulfill the following conditions:
Caution! 3x0.75 mm2 and 10A/250V.
The microscope's electrical accessory components are
not protected against water. Water can cause electric
shock.

Caution!
Protect the microscope from excessive temperature
fluctuations. Such fluctuations can lead to the accumu-
lation of condensation, which can damage the electrical
and optical components.
Operating temperature: 15-35°C

WARNING
Before exchanging the fuses or lamps, be absolutely
certain to switch off the main power switch and remove
the power cable.

10
3. SAFETY NOTES

3.3 Laser safety Two equivalent interlock connectors, which lead to the
microscope's interlock switch, are attached to the back of
3.3.1 Safety switch (interlock) on the microscope
the microscope. The interlock connector of the microscope
Two safety switches are integrated into the microscope us- must be connected to your laser beam interrupter.
ing a laser safety kit option. These must be integrated into
the laser safety concept so that the light path of the laser
beam is interrupted when the interlock switch is opened.

Interlock connectors on the rear side of the stand

Technical data for the interlock connector:

1 Plug:
Manufacturer: Binder
Type: 09-0097-20-05
(5-pin flange plug,
711 series subminiature round plug connector)

Compatible coupling:
Manufacturer: binder
Type: 99-0096-102-05
(5-pin cable socket,
2 711 series subminiature round plug connector)

Type: 79-1414-12-05
(5-pin cable socket with 2m cable,
Interlock switch (1) and (2) 702 series subminiature round plug connector)

Interlock switch 1: Type: 99-0414-00-05

An interlock switch is integrated on the Transmitted Light (5-pin cable socket,
arm and activated when the Transmitted Light arm is folded 712 series subminiature round plug connector)
back (interlock switch opens).
Pin assignment:
Interlock switch 2: Switching contact between pin 1 and pin 5.
An additional interlock switch is also installed in the micro-
scope, which is activated (interlock switch opens) when Maximum permitted electrical characteristic values of the
the beam path to the eyepiece is released. integrated interlock switch:
Umax= 40 V DC
Imax= 0.2 A

11
3. SAFETY NOTES

3.3.2 Screwed-on filter turret cover 3.3.3 Laser coupling by the user
As a safety precaution on laser microscope systems, the Because of a danger for the user due to laser radiation, the
fluorescent turret disk cover is secured using 2 Leica DMi8 microscopes in the standard version are not
suitable without additional laser safety measures for cou-
DIN 912 M4x6 lens head Allen screws. pling laser radiation1).
(Order No. 11 703 121 630 000)
If you are, however, modifying a microscope and couple a
laser beam into it, as the user, you assume responsibility
as the manufacturer of the complete system.

We do not make provisions for your interference with the

WARNING device and, therefore, you are fully responsible for the
modifications regarding the adherence to basic safety re-
Store the screws in a safe place. Never operate the quirements and laser protective measures set forth by the
microscope when the cover is not screwed into place! European Union guidelines and by the national legal and
technical directives and standards.

If you modify your microscope by coupling a laser beam

without authorization, when determining and implement-
ing laser safety measures, also take note of the subse-
quently described hazards at the microscope, as here the
danger exists of serious and irreversible eye damage and
skin injuries!

WARNING
Danger of serious and irreversible eye damage from la-
ser radiation!
Turn the screws to secure the filter turret cover Radiation of the eye or skin from direct or indirect laser
radiation has to be avoided by all means! The laser light
can cause serious eye damage and skin damage!

1) Laser radiation of Class 1 lasers is exempt. Further details

regarding this topic in Section 3.3.4

12
3. SAFETY NOTES

3.3.3.1 Safety hazard due to folded back

Transmitted Light arm
If you fold back the Transmitted Light arm, the laser radia-
tion escaping from the lenses is beamed directly into the
open space.

Important safety note

As a safety measure, we recommend the microscope
models with integrated interlock connectors as men-
tioned in Section 3.3.3.7. Folded back Transmitted Light
arm; the light output out of the
objective is not shadowed by
the condenser base.

3.3.3.2 Safety hazard at eyepiece

The laser radiation that you coupled into the microscope
can disperse from the eyepiece and directly hit the user's
eye!

Important safety note

As a safety measure, we recommend the microscope
models with integrated interlock connectors as men-
Eyepieces
tioned in Section 3.3.3.7.

3.3.3.3 Safety hazard in the condenser area

during operation.
If you couple a high power laser beam into the microscope,
the laser radiation, broadened by the lens, that passes the
condenser laterally, can exceed the permitted limit values
for eyes and skin.

Increased laser radiation can

beam laterally past the condenser

13
3. SAFETY NOTES

3.3.3.4 Safety hazard above the condenser

The laser beam path between the condenser base and the
illuminated field diaphragm is without obstruction.

Laser beam path between condenser base and illuminated field dia-
phragm without obstruction

3.3.3.5 Safety hazard in the specimen area

The laser radiation is accessible without obstruction in the
specimen area of the microscope!

Specimen area

14
3. SAFETY NOTES

3.3.3.6 Safety hazard due to openings at microscope

Furthermore, the terminals that are not occupied by objec-
Make sure, as part of your risk assessment, that all open-
tives have to be closed with caps at the objective nose-
ings at the microscope are tightly sealed and can only be
piece.
opened with tools to prevent laser radiation from escaping!

The openings to consider, for example: top port next to the

eyepieces, left and right side port, bottom port, infinity port,
connection for Transmitted Light lamp housing at the arm,
lamp connection for fluorescence/connection for incident
light lamp housing, cover for fluorescent turret disk.

The material that is used to seal off the openings has to be

suitable for the intensity of the coupled laser beam.

Openings at the stand that have to be tightly sealed

a Cover for fluorescent turret disk
b Transmitted Light lamp housing
c Incident light lamp housing
d Side port right j
e Bottom port
f Side port, left
g Objective nosepiece
h Top port
i Infinity port
j Openings at objective nosepiece

a
i
c g
f

d
e

15
3. SAFETY NOTES

3.3.3.7 Note regarding the safety hazards in Sections a) Safety function for the safety hazard mentioned
3.3.3.1 and Section 3.3.3.2 in Section 3.3.3.1.
The special microscope variants have a built-in interlock
switch on the Transmitted Light arm that is activated when
Important safety note
folding back the Transmitted Light arm (interlock switch
The overall responsibility for laser safety of the laser opens).
microscope system assembled by you remains with you The interlock connector of the microscope must be con-
as the responsible body, even if you use the microscopes nected to your laser beam interrupter, which interrupts the
from Leica Microsystems CMS GmbH listed below in laser beam coupled into the microscope when opening the
conjunction. Within the context of your system composi- interlock switch or folding back the illumination arm.
tion and modifications, you have to verify whether the
component selected by you from this chapter is effec-
b) Safety function for the safety hazard mentioned
tive and suitable for your laser protection concept.
in Section 3.3.3.2.
We recommend that for your laser applications you use An additional interlock switch is also installed in the micro-
a microscope variant with the following selection options scope, which is activated (interlock switch opens) when
because the microscope is already equipped with interlock the beam path to the eyepiece is released.
switches at the factory: The interlock switch of the microscope must be connected
to your laser beam interrupter, which interrupts the laser
Leica No. 11889086 beam coupled into the microscope when opening the in-
Laser Safety Kit terlock switch or releasing the beam path towards the eye-
piece.
and also one of the following options: Please note that the laser radiation may not be coupled via
the bottom port!
Leica No. 11889077
Left motorized side port, 100% and interlock

Leica No. 11889078

Right motorized side port, 100% and interlock

Leica No. 11889079

Right/left motorized side port, 100% and interlock

Leica No. 11889087

Manual side port (100/0), Int.

Leica No. 11889088

Left manual side port (100/0) Bottom port

Both interlock switches a) and b) are routed to a shared in-

Note: terlock connector on the microscope; you have to connect
The fixed Transmitted Light arm (order No. 11525105 (with your laser beam interrupter to this connector.
short cable) or order No. 11525116 (with long cable) is not
suitable for laser applications and therefore is not provided
for these applications.

16
3. SAFETY NOTES

When doing so, note the electrical characteristic values of 3.3.4. Exception for Class 1 lasers
the integrated interlock switches in the microscope. You
Coupling a laser of Class 1 in accordance with IEC/EN
must not exceed these values by connecting a laser beam
60825-1 into a microscope does not result in any danger to
interrupter, as otherwise the interlock switches will be
the user.
damaged and no longer function safely and reliably:
The listed safety hazards apply only for lasers of Class 1M,
2, 2M, 3R, 3B and 4.
Maximum permitted electrical characteristic values of the
integrated interlock switch:
Note that the rules and regulations of the United States FDA
21CFR1040.10 Class I (CFR – Code of Federal Regulation Title
Umax= 40 V DC
21, revised as of April 1, 2011) do not distinguish between
Imax= 0.2 A
Class 1 and 1M. Thus the statement that there are no safety
hazards on the microscope do not apply for the laser clas-
The integrated interlock switches in the microscope and
sification in accordance with FDA Class 1.
their wiring with a laser beam interrupter/laser ensure that
the laser radiation you coupled into the microscope cannot
get into the user's eyes through the eyepieces.

Important safety note

In addition, observe the safety hazards described in
Section 3.3.3.3 to 3.3.3.6!

17
3. SAFETY NOTES

3.5 Storage and transport Weight

Transport CAUTION
At a full load, the microscope weighs more than 18
Caution! kg. The user must take corresponding precautions for
Transport and storage at -20 °C – +85 °C and max. 90% transporting the instrument.
humidity.

For shipping or transporting the microscope and its acces- Caution!

sory components, the original packaging should be used.
For transport, always remove all components listed un-
der "Transport!".
As a precaution to prevent damage from vibrations, the fol-
lowing components should be disassembled and packaged
separately:
3.6 Ambient conditions
t
6OTDSFX
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PCKFDUJWFT
Caution!
t
4FDVSF
UIF
PCKFDUJWF
OPTFQJFDF
FH
XJUI
BEIFTJWF
UBQF
This microscope may not be used at elevations over
t
3FNPWF
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FZFQJFDFT 2000 m above sea level.

t
3FNPWF
UIF
DPOEFOTFS

t
3FNPWF
UIF
TQFDJNFO
TUBHF
Caution!
t
3FNPWF
UIF
5SBOTNJUUFE
-JHIU
BSN Do not use this instrument near sources of high electro-
magnetic radiation (for example, unshielded, intention-
t
3FNPWF
UIF
MBNQ
IPVTJOHT ally operated ultra-high frequency sources), because
these can disrupt proper operation.
t
4FDVSF
UIF
NBHOFUJD
nVPSFTDFOU
UVSSFU
DPWFS
FH
XJUI

adhesive tape. We recommend assessing the electromagnetic environ-
ment before operation of this instrument and then giving
t
3FNPWF
BMM
NPWJOH
PS
MPPTF
QBSUT corresponding instructions.

t
1SPUFDU
UIF
NJDSPTDPQF
GSPN
EVTU
BOE
EJSU
VTJOH
B
QMBTUJD

bag.

18
3. SAFETY NOTES

Installation location 3.7 Notes on handling light sources

Work with the microscope should be performed in a dust-
free room, which is free of oil vapors, other chemical va- CAUTION
pors and extreme humidity. At the workstation, large tem-
perature fluctuations, direct sunlight and vibrations should Light sources pose a potential irradiation risk (glare, UV
be avoided. These conditions can distort measurements radiation, IR radiation). Therefore, lamps may only be
and longer microscopic scans. operated in closed housing and in an installed condition.
Never look directly into the beam path (blinding hazard).
Permitted ambient conditions:
Temperature 15–35°C+)
Relative humidity maximum 90% up to 30°C
non-condensing CAUTION
Do not switch on the light source until the light guide is
Microscopes in warm and warm-damp climatic zones re- connected to the microscope.
quire special care in order to prevent fungus contamination. Uncontrolled light output from the light guide poses a
For additional instructions, see the chapter on "Care of the blinding hazard!
Microscope" oS. 89 There is also a burn hazard at the light guide output!

Caution!
CAUTION
The microscope must be set up so that the power volt-
age input is not obstructed and the instrument can Make sure to follow the safety notes in the operating
quickly be removed from the power supply if necessary. manual for the light source.

Caution!
CAUTION
Electrical components must be placed at least 10  cm Switch on the light source only if it is firmly connected to
away from the wall and from flammable substances. the microscope.
Uncontrolled light output poses a blinding hazard!

CAUTION
Do not look into the eyepieces when toggling the con-
trast method!
During the toggling procedure, the maximum radiant
power of the light source may be present on the eye-
pieces for a short time and can cause blindness in the
user!

+) Up to 40°C in microscope climate chambers sold by Leica

Microsystems GmbH

19
3. SAFETY NOTES

3.11 Disposal
CAUTION Once the product has reached the end of its service life,
please contact Leica Microsystems GmbH Service or Sales
Lamps and lamp housing may be hot! about disposal.
They must be placed at least 10 cm away from the wall
and away from flammable substances. Please observe and comply with the national and federal
Supply and data lines in particular may not come into laws and regulations that are equivalent to EC directives
contact with lamp housings! such as WEEE.

3.8 Notes on handling the Touch Screen

Note!
Like all electronic devices, the microscope, its acces-
sory components and consumables must never be dis-
Caution!
posed of with general household waste.
Touch the touch screen using your finger only. Never
use a pen or other hard, sharp or pointed objects.

3.9 Notes on handling immersion oil

Caution!
When using immersion oil, take care to avoid skin con-
tact! Ask the supplier for a safety data sheet!

3.10 Notes on handling acids and bases

For examinations using acids or other aggressive chemi-
cals, particular caution must be taken.

Caution!
Be absolutely certain to avoid direct contact with these
chemicals.

20
4. OVERVIEW OF THE INSTRUMENT

4. Leica DMi8 configurations instrument overview

4.1 Microscope configurations (examples)

Leica DMi8 with manual components Leica DMi8 with manual components
With a fixed Transmitted Light arm With a tiltable Transmitted Light arm

Leica DMi8 with encoded components Leica DMi8 with at least one motorized component

21
4. OVERVIEW OF THE INSTRUMENT

4.2 Dimensions (specified in mm)

Leica CTR compact

162.5
168.1
195 130
204

Weight: approx. 2 kg

Leica CTR Advanced/Advanced+

310 215

275.6
270

326

Weight: approx. 4 kg

22
4. OVERVIEW OF THE INSTRUMENT

Leica DMi8 (with tiltable Transmitted Light arm)

670
500 281

542 310

Weight: approx. 19 kg

Height compensation plate*

In order to enlarge the viewing height by 23 mm, raise the
side camera ports for very large cameras or spinning disks,
or to use a microscope with an inactive bottom port even
without a hole in the work table, a height compensation
plate (order No. 11525200) was developed.

23
4. OVERVIEW OF THE INSTRUMENT

4.3 Specifications+)
Contrast methods Leica DMi8 series - depending on Transmitted Light arm
t
5SBOTNJUUFE
-JHIU
5-



#'
%'
1)
%*$
1PM
t
*OUFSNFEJBUF
FZFQPJOU


*.$
*OUFHSBUFE
.PEVMBUJPO
$POUSBTU

IPH (Integrated Phase Contrast)

t
*ODJEFOU
MJHIU
*-


'MVP

Leica DMi8 with motorized condenser and motorized fluorescence

t
$PNCJOBUJPO
5-*-

'MVP%*$
'MVP1)
Transmitted Light arm Tiltable Transmitted Light arm
t

.BOVBM
BOE
FODPEFE
t


8JUI
JOUFHSBUFE
NFDIBOJDBM
UJMUJOH
NFDIBOJTN
XJUI
TVGmDJFOU
SPPN
GPS
TQFDJNFOT
BOE

micromanipulators
t

'PS
≤ S40 condensor with an integrated manual or motorized illuminated field dia-
phragm
t

'JMUFS
NBHB[JOF
GPS

SFQMBDFBCMF
mMUFST
t
8JUI
DPOEFOTFS
RVJDL
DIBOHF
t

.BOVBM
TIVUUFS
t

-BNQ
IPVTJOH
SFDFQUBDMF
GPS
SFQMBDFBCMF
-&%T
t

8JUI
JOUFHSBUFE
DBCMF
EVDU
t

$POEFOTPS
TUPQ
UP
QSFWFOU
GSPN
CFJOH
QVTIFE
EPXO
BDDJEFOUBMMZ

or
Fixed Transmitted Light arm
t
With integrated LED illumination
t

.BOVBM
BEKVTUNFOU
PG
CSJHIUOFTT
BOE
BQFSUVSF
EJBQISBHN
t

"VUPNBUJD
CSJHIUOFTT
BEKVTUNFOU
XIFO
DIBOHJOH
UIF
DPOUSBTU
NFUIPE
t

"VUPNBUJD
TIVUPGG
BEKVTUBCMF

t
Integrated condenser holder

t
Receptacle for Transmitted Light filter

+) Depending on configuration

24
4. OVERVIEW OF THE INSTRUMENT

Fluorescence axis Manual, external

t
.BOVBM
TIVUUFS
t
.BOVBM
mFME
BOE
BQFSUVSF
EJBQISBHN
t
3FDFQUBDMF
GPS
MJHIU
HVJEF
t
3FDFQUBDMF
GPS
&'8
FYUFSOBM
mMUFS
XIFFM

t
3FDFQUBDMF
GPS
TUSVDUVSFE
JMMVNJOBUJPO
0QUJHSJE

t
.BOVBM
nVPSFTDFODF
JOUFOTJUZ
NBOBHFS
'*.

(Reduces the light intensity of the incident light illumination)

or
Manually integrated
t

.BOVBM
TIVUUFS
t
.BOVBM
mFME
BOE
BQFSUVSF
EJBQISBHN
t
3FDFQUBDMF
GPS
MJHIU
HVJEF
t
-BNQ
IPVTJOH
SFDFQUBDMF
GPS
FYUFSOBM
MBNQ
IPVTJOH
t
.BOVBM
nVPSFTDFODF
JOUFOTJUZ
NBOBHFS
'*.

(Reduction of light intensity from the incident light illumination)

or
Motorized
t
.PUPSJ[FE
mFME
BOE
BQFSUVSF
EJBQISBHN
t
.PUPSJ[FE
TIVUUFS
WJB
'*.
t
3FDFQUBDMF
GPS
MJHIU
HVJEF
BEBQUFS
t
.PUPSJ[FE
nVPSFTDFODF
JOUFOTJUZ
NBOBHFS
'*.

(Reduction of light intensity from the incident light illumination)

Infinity port and T-Houses t
8JUI
B
NJSSPS
GPS
TXJUDIJOH
CFUXFFO
UIF
MFGU
PS
SJHIU
BOE
UIF
SFBS
QPSUT
or
t
8JUI
QSJTN
With 2 possible positions:
50% rear port/50% left/right port
80% rear port/20% left/right port
100% rear port/0% left/right port
Additional T-Houses:
1. No fluorescence, holder for Transmitted Light arm
2. One infinity port aperture in the rear
3. Special with integrated fluorescence
4. Special port for TIRF/GSD
Fluorescence t
QPTJUJPO
nosepiece disk t
&ODPEFE
NBOVBMMZ
PS
FMFDUSPOJDBMMZ
XJUI
DPMPSDPEFE
SJOH
or
t
NPUPSJ[FE
Tube t
&SHPOPNJD
XJUI
PS
XJUIPVU
QIPUP
PVUQVU
PO
UIF
MFGU
TJEF
t

TXJUDIJOH
QPTJUJPOT

7*4
BOE
7*4$".
PS
t

TXJUDIJOH
QPTJUJPOT

7*4
BOE
7*4$".
t
0QUJPOBM
XJUI
#FSUSBOE
MFOT
t
*OUFSQVQJMMBSZ
EJTUBODF
DPOUSPM
t
)FJHIU
BOE
BOHMF
BEKVTUNFOU
¡

¡

25
4. OVERVIEW OF THE INSTRUMENT

Magnification Changer Motorized

t

PS

TXJUDI
QPTJUJPOT

Magnification factors 1x; 1.6x; 2x or 1x; 1.6x or 1x; 2x
t
8PSLT
PO
BMM
DBNFSB
QPSUT
BOE
FZFQJFDFT
or
Manual
t

TXJUDI
QPTJUJPOT

Magnification factors 1x; 1.6x
t
8PSLT
PO
UIF
UVCF
QPSU
BOE
FZFQJFDFT
Objective turret motorized
t
QPTJUJPO
GPS
objectives with M25 threads and a 45mm parfocalizing distance
t
'PS
%*$
NPUPSJ[FE
PS
NBOVBMFODPEFE
8PMMBTUPO
QSJTN
UVSOUBCMF
t
8JUI
TQMBTI
QSPUFDUJPO
or
encoded
t
QPTJUJPO
GPS
PCKFDUJWFT
XJUI
.
UISFBET
BOE
B
NN
QBSGPDBMJ[JOH
EJTUBODF
t
'PS
%*$
NPUPSJ[FE
PS
NBOVBMFODPEFE
8PMMBTUPO
QSJTN
UVSOUBCMFT
or
Manual
t
QPTJUJPO
GPS
PCKFDUJWFT
XJUI
.
UISFBET
BOE
B
NN
QBSGPDBMJ[JOH
EJTUBODF
t

For DIC: manual Wollaston prism turntable
Stages t
'JYFE
TUBHFT
t
4UBHF
QMBUF
DPBUFE
JO
DFSBNJD

NN
Y

NN

t Heated stage plate (3°C above room temperature up to 60°C) (248 x 212 mm)
t
5FNQFSBUVSFBEKVTUBCMF
TUBHF
QMBUF
¡$
UP
¡$


NN
Y

NN

t
'JYFE
NJDSPNBOJQVMBUJPO
TUBHFT
t
4UBHF
QMBUF
DPBUFE
JO
DFSBNJD

NN
Y

NN


t
)FBUFE
TUBHF
QMBUF
¡$
BCPWF
SPPN
UFNQFSBUVSF
VQ
UP
¡$


NN
Y

NN


t
5FNQFSBUVSFBEKVTUBCMF
TUBHF
QMBUF
¡$
UP
¡$


NN
Y

NN

t
.BOVBM
BOE
NPUPSJ[FE
QMBUF
DSPTTTUBHF
t
5SBWFM
SBOHF

NN
Y

NN
t
0QUJPOBM
JOTFSUT
OPSNBM
IFBUBCMF
BOE
DPPMBCMF

GPS
EJWFSTF
BQQMJDBUJPOT

Size of inserts:160 mm x 110 mm (compatible with scanning stages)
t
4DBOOJOH
TUBHF
t
0QUJPOBM
JOTFSUT
OPSNBM
IFBUBCMF
BOE
DPPMBCMF

GPS
EJWFSTF
BQQMJDBUJPOT
Size of inserts:160 mm x 110 mm
t
-.5

MJOFBS
NPUPSJ[FE
TUBHF

26
4. OVERVIEW OF THE INSTRUMENT

Condensers For tiltable Transmitted Light arms:

t
.PUPSJ[FE
BOE
FODPEFE
PS
NBOVBM
BOE
FODPEFE
t
.PUPSJ[FE
PS
NBOVBM
BQFSUVSF
EJBQISBHN
t
$POUSBTU
NFUIPE
#'
%'
1)
%*$
1PM
*.$
*1)
t
"VUPNBUJD
NFUIPE
UPHHMJOH
t
$POEFOTFS
EJTL
XJUI

PS

QPTJUJPOT
GPS
DPOUSBTU
NFUIPET
t

DPOEFOTPS
IPVTJOHT
4o4
BOE
4
4

t
$POEFOTFS
IFBET
4
PJM
4
ESZ
4
4
4

t
$POEFOTFS
IFBET
DBO
CF
TXVOH
PVU
t
"MM
DPOEFOTFST
GPS
NBHOJmDBUJPOT
PG
Y
UP
Y
t
8JUI
PS
XJUIPVU
NPUPSJ[FE
PS
NBOVBM
QPMBSJ[FS
PQUJPOBM

t
8JUI
NPUPSJ[FE
PS
FODPEFE
8PMMBTUPO
QSJTN
EJTL
PQUJPOBM

For the fixed Transmitted Light arm

t
4
DPOEFOTFS
t
4
DPOEFOTFS
Z-focus Leica DMi8 motorized
t
.PUPSJ[FE
BOE
FODPEFE
t

NN
USBWFM
QBUI
NN
CFMPX

NN
BCPWF
UIF
TUBHF

t
.BYJNVN
USBWFM
TQFFE
NNT
t

GPDVT
JODSFNFOUT

˜N

˜N

˜N

˜N

˜N
t
&MFDUSPOJD
GPDVT
SFQPTJUJPOJOH

t
"VUPNBUJD
MPXFSJOH
CFGPSF
PCKFDUJWF
DIBOHF
t
&MFDUSPOJD
QBSGPDBMJUZ
t
0QUJPOBM
"EBQUJWF
'PDVT
$POUSPM
"'$

t
0QUJPOBM
$MPTFE
-PPQ
SFQPTJUJPOJOH
BCJMJUZ
≤ 20 nm)

t
)BOEXIFFMT
FBDI
XJUI

JODSFNFOUT
GPS
DPBSTF
BOE
mOF
GPDVTJOH

JODSFNFOUT

Leica DMi8 manual

t
.BOVBM
t

NN
USBWFM
QBUI
NN
CFMPX

NN
BCPWF
UIF
TUBHF

t
)BOEXIFFMT
FBDI
XJUI

JODSFNFOU
GPS
DPBSTF
BOE
mOF
GPDVTJOH

JODSFNFOUT

Observation ports Leica DMi8 motorized

t
.PUPSJ[FE
BOE
FODPEFE
t
-FGU
TJEF
QPSUT


PS

USBOTNJTTJPO

t
0QUJPOBM
3JHIU
TJEF
QPSUT


PS

USBOTNJTTJPO

t
0QUJPOBM
#PUUPN
QPSU
t
0QUJPOBM
5PQ
QPSU
XJUI

TXJUDI
QPTJUJPOT
t

UP
UIF
FZFQJFDFT

t

UP
UIF
FZFQJFDFT
UP
UIF
QPSU

Leica DMi8 manual

t
.BOVBM
t
-FGU
TJEF
QPSU

PS

USBOTNJTTJPO

t
0QUJPOBM
5PQ
QPSU
XJUI

TXJUDI
QPTJUJPOT
t

UP
UIF
FZFQJFDFT

t

UP
UIF
FZFQJFDFT
UP
UIF
QPSU

27
4. OVERVIEW OF THE INSTRUMENT

Controls Depending on level of motorization

t
5PVDI
4DSFFO
POMZ
GPS
NPUPSJ[FE
-FJDB
%.J

t
#VUUPO
GPS
5-'MVPS
UPHHMJOH
t

CVUUPOT
GPS
NPUPSJ[FE
BQFSUVSF
EJBQISBHN
BEKVTUNFOU
t

CVUUPOT
GPS
NPUPSJ[FE
JMMVNJOBUFE
mFME
EJBQISBHN
BEKVTUNFOU
t

CVUUPO
GPS
PQFSBUJOH
UIF
NPUPSJ[FE
TIVUUFS
t

WBSJBCMF
GVODUJPO
LFZT
t

PQFSBUJOH
CVUUPOT
GPS
GPDVT
UISFTIPMET
POMZ
GPS
FMFDUSPOJD
GPDVTJOH

t

IBOEXIFFMT
GPS
GPDVTJOH
t
3PUBSZ
LOPC
GPS
TFUUJOH
UIF
CSJHIUOFTT
t

CVUUPOT
GPS
nVPSFTDFODF
DVCFT
t

CVUUPOT
GPS
PCKFDUJWFT
t
-FJDB
4NBSU.PWF
FSHPOPNJD
DPOUSPM
FMFNFOU
GPS
NPOJUPSJOH
Y Z [
BOE

BEEJUJPOBM
WBSJ-
able function keys
t
-FJDB
451
PQFSBUJPO
WJB
FYUFSOBM
5PVDI
4DSFFO
t
-FJDB
451
PQFSBUJPO
WJB
5PVDI
4DSFFO
VTJOH
DPOUSPM
FMFNFOU
GPS
NPOJUPSJOH
Y Z [
BOE

additional variable function keys
t
4XJUDI
SPET
GPS
PQFSBUJOH
UIF
QPSU
NBOVBM
TIVUUFS
BOE
.BHOJmDBUJPO
$IBOHFS
t
4MJEFS
GPS
NBOVBM
'*.
*.$
BOE
*1$
BEKVTUNFOU
t
4XJUDI
SPE
GPS
PQFSBUJOH
UIF
JOmOJUZ
QPSU
Electronics box t Separate unit for controlling all motorized and electronic elements of the
microscope
Alternatively:
t
-FJDB
$53
Compact
t
-FJDB
$53
"EWBODFE
t
-FJDB
$53
"EWBODFE
XJUIPVU
QPXFS
TVQQMZ

t
-FJDB
$53
"EWBODFE
Interfaces t
4FSWJDF
QPSU
GPS
TBWJOHMPBEJOH
NJDSPTDPQF
TFUUJOHT
WJB
UIF
5PVDI
4DSFFO

Leica DMi8 motorized
t

Y
*¤$
GPS
FYUFSOBMJOUFSOBM
QFSJQIFSBM
EFWJDFT
t
$53
CPYFT
t

Y
JOUFSMPDL
GPS
MBTFS
BQQMJDBUJPOT

4.4 Controls
Depending on the configuration of your microscope, vari-
ous controls are available to you. Accordingly, the control
panel fields on the left and right sides of the stand contain
a different number of buttons. However, the functions them-
selves are assigned to specific buttons.

The controls are displayed below using typical examples

for the manual, encoded and fully automated microscope
variant.

The Touch Screen is available as a control element on the

Leica DMi8 with at least one motorized component.

The external controls are the Leica SmartMove control and

the Leica STP4000 and Leica STP8000 control panels.

28
4. OVERVIEW OF THE INSTRUMENT

4.4.1 Control panel fields

Control panel field on the left microscope side

1 Motorized aperture diaphragm adjustment
2 Motorized illuminated field diaphragm adjustment
5
3 Motorized toggling between the TL/Fluor illumination axes

4
4 Adjusting the Transmitted Light brightness of the inten-
sity levels for the automated FIM (fluorescence inten-
sity manager) 3
6 2 1
An LED indicates the illumination method which is cur-
rently affected by the brightness adjustment.
Maximum system design of the left control panel field
5 Opening/closing the motorized shutter The number of buttons may vary depending on the configu-
or during manual shutter light control ration.
An LED displays the state of the shutter:
LED on = shutter opened
LED off = shutter closed.
6 Focusing

Control panel field on the right microscope side

1 Variable function keys (freely configurable)
The function keys are preassigned at the factory (see
"Identification Sheet" and S. 92 1 4

2 Focus threshold (the LED illuminates when the focus

threshold is set) 3

3 Setting the focus position and focus threshold

4 Focus position (the LED illuminates when the focus po- 2 5
sition is set)
5 Focusing
Maximum system design of the right control panel field
The number of buttons may vary depending on the configu-
ration.

Control panel fields on the front side

Swiveling Touch Screen for adjusting all automated compo-
nents on the Leica DMi8.
Adjustable angle for better readability.
Refer also to the chapter titled "The Touch Screen" S. 34

29
4. OVERVIEW OF THE INSTRUMENT

Encoded objective nosepiece

1 6 LEDs for indicating the current objective 1

2
1
2 Label fields +)

Encoded objective nosepiece and encoded fluo turret 3

2
1
1 6 LEDs for indicating the filter cube
2 Label fields +)
3 6 LEDs for indicating the current objective

Motorized objective nosepiece

1
1 6 objective changer buttons with LEDs for indicating the 2
1
current objective
2 Label fields+)

Motorized objective nosepiece and motorized fluo turret 2 3

1
1 6 filter cube changer buttons with LEDs for indicating
the current filter
2 Label fields+)
3 6 objective changer buttons with LEDs for indicating the
current objective

+) Stickers for labeling are included in the delivery package

30
4. OVERVIEW OF THE INSTRUMENT

4.4.2 Additional control elements

Left side of the Leica DMi8 with a fixed Transmitted Light
arm

1 Filter receptacle 1
2
2 Aperture Diaphragm Adjustment
3 4
3 Stop lever for condenser height adjustment
4 IMC or light ring slide
5 Bertrand lens centering
6 Toggling the top port and Bertrand lens 5
7 Manual objective change 6

8 Left side port

9 Manual filter change
10 Focusing
11 Brightness adjustment
12 IMC module

7 8 9 10 11 12

Left side of the Leica DMi8 with a tiltable Transmitted Light

arm

1 Illuminated field diaphragm adjustment (motorized or

manual)
2 Condenser height adjuster
3 Swinging in the polarizer
4 Aperture Diaphragm Adjustment
1
5 Condenser setting
6 Condenser height stop

2 3

4
5
6

31
4. OVERVIEW OF THE INSTRUMENT

DIC setting

1 DIC fine adjustment

2 Selecting the objective prisms

1 2

Manual DIC objective prism disk

Motorized DIC objective prism disk

Right side of the Leica DMi8 with a tiltable Transmitted

Light arm
1
1 Switching the Transmitted Light filter on and off
The front position is assigned at the factory using a
shutter (dark stop), which is interchangeable with a fil- 2
ter.
2 Tube setting
3 Toggling the infinity port
4 Analyzer slot
5 Identification label
6 Right side port
7 Cover for fluorescent turret disk
8 Focusing
9 Focus thresholds
3
10 Variable function keys 4

10 9 8 7 6 5

32
4. OVERVIEW OF THE INSTRUMENT

Right side of Leica DMi8 with manual integrated fluores-

cence axis

1 Manual Magnification Changer

2 Focusing
3 Manual filter change
4 Receptacle for analyzer
5 Toggling the left side port
6 Stage movement
7 On/off switch (only on manual stands)
8 Illuminated field diaphragm adjustment 10
9 Manual setting for FIM
11
10 Centering illuminated field diaphragm
11 Manual shutter
9
8

1 2 3 4 5 6 7

10

9
8
11

Section of manual integrated fluorescence axis

Manual external fluorescence axis

(Alternative connection on the left or rear microscope
side)

1 Connection for light guide

2 FIM
3 Diaphragm module
4 Receptacle for EFW or structured illumination 1 2 3 4 5

5 Manual Shutter

33
4. OVERVIEW OF THE INSTRUMENT

4.4.3 The Touch Screen

The Touch Screen adjusts all motorized components of
the Leica DMi8 automatically via buttons. After the device
is switched on, the display shows the current microscope
status. The display and functions that can be operated us-
ing the Touch Screen depend on the features of the indi-
vidual microscope.
The meanings of the most important pictograms are listed
in the Appendix o S. 95

The display shows various levels of operation:

Level 1: Navigation bar

Level 2: Menu bar
Level 3: Control panel field

When you select a pictogram in the navigation bar (on the

left-hand border of the display), the corresponding menu
bar (on the top border of the display) is shown. The display
on the control panel changes accordingly. For some func-
tions, an additional information field appears above the op-
erating keys.

The navigation bar allows you to quickly move between

navigation items:

Initialization
Basic microscope settings

Navigation bar Control panel Menu bar

Contrast methods

Magnification

Stage and focus controls

Optional

Touch screen settings

Touch Screen after initialization

34
4. OVERVIEW OF THE INSTRUMENT

Note:
Before working with the touch screen, we recommend cali-
brating it first.

Calibration

t
1SFTT
UIF
button.

t
1SFTT
UIF
button.

t
'PMMPX
UIF
JOTUSVDUJPOT
BOE
QSFTT
BMM

DPSOFST
PG
UIF

working area using your finger. Press and hold the cali-
bration cross until the next cross is displayed. This ad-
justs the sensitivity of the Touch Screen and the pressure
surface to the user.

Other Touch Screen settings:

Language selection
Version number
The current language for the display texts is shown, and
you can change it by selecting the corresponding flag.

Saving the microscope settings This key shows you the version number of the Touch Screen
In Microscope Settings, press Save to write the cur- software and that of the master firmware of the connected
rent microscope settings to a USB stick, inserted into microscope.
the left side of the microscope (Service port). Pressing Please have these available for any inquiries.
Load restores all settings.

Display brightness Note:

To prevent unwanted interference from light, you can The meaning of the other menu items of the navigation bar
switch off the display using the Switch off key. To switch it is explained in the respective chapters.
back on, simply touch any location on the display.
You can also change the brightness incrementally using the
Brightness key.

35
4. OVERVIEW OF THE INSTRUMENT

4.4.4 The Leica SmartMove control element

Rotary knobs on the SmartMove
The rotary knobs 1 and 2 move the specimen stage in the x
and y-direction.
The image is focused using the rotary knob 3.

You can adjust the height of the rotary knobs by turning the
wheel 4 to a working height that is convenient for you.

Variable function keys on the SmartMove

The variable function keys are assigned functions at the
factory that are appropriate to the features of your micro-
scope. They are labeled accordingly. For details on key as-
signments, please refer to the included identification sheet.
For information on the abbreviations used, please refer to
the list oS. 94

Leica SmartMove control

1 Movement in x-direction
Note:
2 Movement in y-direction
The key assignment can be changed using the LAS X soft-
ware. 3 Focus setting
4 Individual setting of the knob height position
5 Variable function keys (preset at the factory)
Note: 6 Status LED
To lock the controls, press and hold the two front keys (5)
simultaneously for 5 seconds. Repeat this process to unlock
the controls. The status LED (6) indicates whether the Leica
SmartMove is in normal mode or locked mode.
Red LED = Entries blocked
Green LED = Normal mode

1
3

2 4
5

36
4. OVERVIEW OF THE INSTRUMENT

4.4.5 Leica STP4000/8000 external control panel Leica STP4000

The Leica STP4000 enables remote control of all automated
microscope functions using an external Touch Screen. The
Leica STP8000 control panel also enables monitoring in X, Y
and Z and has user-definable function keys.
Separate instructions are provided for operation of the
Leica STP4000/8000.

Controls of the Leica STP8000

1 Touch Screen
2 Information key
3,4 Variable function keys, user-programmable
5 Fine focus adjustment
6 Coarse focus adjustment
7 Movement in y-direction
8 Movement in x-direction

Navigation bar Control panel Menu bar

2 1 8

3 4 5 6
Touch Screen for STP4000/8000
37
5. OPERATION

5. Operation

Caution! Note:

The microscope may only be set up, installed and put Operating the motorized components using the Leica
into operation by authorized Leica personnel! STP4000/8000 control panel is described in separate In-
structions for Use.

Note:
Caution!
For information about using the LAS X software, refer to the
The microscope may only be operated by personnel who corresponding online help.
have been instructed by an authorized Leica employee!

Note:
If at all possible, avoid touching optical surfaces such as
the lens surfaces on the objective. If fingerprints do appear
on the glass surfaces, remove them with a soft leather or
linen cloth. Even small traces of finger perspiration can
damage the surfaces in a short time. For additional instruc-
tions, see the chapter entitled "Care of the Instrument" o S.
89

38
5. OPERATION - SWITCHING ON

5.1 Switching on the microscope

Leica DMi8 without motorized components and without
Leica CTR electronics box

t

4XJUDI
PO
UIF
NJDSPTDPQF
BU
UIF
on/off switch on the

right side of the stand. The indicator lamp is illuminated in
green during operation.

Leica DMi8 with at least one motorized component and

Leica CTR electronics box

t

4XJUDI
PO
UIF
FMFDUSPOJDT
CPY
PO
UIF
POPGG
TXJUDI

5IF

indicator lamp is illuminated in green during operation.

Note:
If you have connected a PC, switch on the electronics box
first, and then the computer.

All motorized microscope components will then run through

an initialization phase.

Note:
At initialization, all of the user's most recent settings are re-
stored.

! Caution:
The focus position and lower threshold are stored when
switching off the microscope.

39
5. OPERATION - SWITCHING ON

After the initialization is completed, the Touch Screen

shows the status screen with the current microscope set-
ting.

For fluorescence:
t
4XJUDI
PO
UIF
Leica EL6000 compact light source on the
on/off switch. The indicator lamp is illuminated during op-
eration.

CAUTION
Do not switch on the light source until the light guide is
connected to the microscope.
Uncontrolled light output from the light guide poses a
blinding hazard!
There is also a burn hazard at the light guide output!

Caution!
When using other light sources, observe the respective
instructions for the light source.

40
5. OPERATION - ILLUMINATION

5.2 Illumination

Caution!
When using other light sources than those described
here, observe the respective instructions for the light
source.

5.2.1 Transmitted Light

t

*G
OFDFTTBSZ
mSTU
TXJUDI
PWFS
UP
UIF
nVPSFTDFODF
BYJT

(TL) by closing the shutter (push in the switch rod for the
manual shutter or press the shutter button for motorized
shutter).

t
4FU
UIF
light intensity using the knob on the left side of the

stand.

t
*G
UIF
NJDSPTDPQF
IBT
B
NBOVBM
TIVUUFS
BOE
B
TIVUUFS
CVU-
ton, the light is switched off when pressing the button. To
switch it on again, press the rotary knob for the light in-
tensity.

Leica DMi8 with automated fluorescence

t

*G
OFDFTTBSZ
TXJUDI
UP
UIF
5SBOTNJUUFE
-JHIU
BYJT
VTJOH

the TL/Fluor key (TL).

t
4FU
UIF
MJHIU
JOUFOTJUZ
VTJOH
UIF
LOPC
PO
UIF
MFGU
TJEF
PG
UIF

stand.

41
5. OPERATION - ILLUMINATION

Adjusting the Transmitted Light brightness using the Touch

Screen:
t

*G
OFDFTTBSZ
TXJUDI
UP
UIF
5SBOTNJUUFE
-JHIU
BYJT
5-

VT-
ing the TL key.

t
"EKVTU
UIF
JOUFOTJUZ
VTJOH
+ and –.
The setting can be made in coarse and fine steps in a
range from 0 to 255. Pressing the middle key, which also
displays the current value, switches between coarse and
fine adjustment.

t
The intensity is individually adjusted and stored for each

objective and contrast method.

5.2.2 Fluorescence
Leica DMi8 with manual encoded fluorescence

t

*G
OFDFTTBSZ
mSTU
TXJUDI
PWFS
UP
UIF
nVPSFTDFODF
BYJT

(Fluor) by opening the shutter (pull out switch rod at the
manual integrated fluorescence axis or on the manual ex-
ternal fluorescence axis).

t
4FU
UIF
MJHIU
JOUFOTJUZ
PO
UIF
Leica EL6000 compact light

source.
(See separate Instructions for Use).

CAUTION
Do not switch on the light source until the light guide is
connected to the microscope.
Uncontrolled light output from the light guide poses a
blinding hazard!
There is also a burn hazard at the light guide output!

42
5. OPERATION - ILLUMINATION

t
8IFO
VTJOH
UIF
Fluorescence Intensity Manager (FIM):

The brightness is adjusted in 5 defined increments (FIM)
using a slider:
100% / 55% / 30% / 17% / 10%

Note:
If the Leica DMi8 is equipped with a 2 port infinity port (oS. 44)
switch over to the current port .oS. 58

Manual integrated fluorescence axis

Manual external fluorescence axis

Leica DMi8 with motorized fluorescence

t
If necessary, switch over to the fluorescence axis (Fluor)

with the TL/Fluor button.

t
4FU
UIF
MJHIU
JOUFOTJUZ
PO
UIF
Leica EL6000 compact light

source.
(See separate Instructions for Use).

CAUTION
Do not switch on the light source until the light guide is
connected to the microscope.
Uncontrolled light output from the light guide poses a
blinding hazard!
There is also a burn hazard at the light guide output!

43
5. OPERATION - ILLUMINATION

t
8IFO
VTJOH
UIF
Fluorescence Intensity Manager (FIM):

The brightness is adjusted in 5 defined increments on the
brightness adjusting knob:
100% / 55% / 30% / 17% / 10%

Setting the fluorescence using the Touch Screen:

t

*G
OFDFTTBSZ
TXJUDI
PWFS
UP
UIF
nVPSFTDFODF
BYJT
'MVPS


with the IL button.

t
8IFO
VTJOH
UIF
'MVPSFTDFODF
*OUFOTJUZ
.BOBHFS
'*.

The brightness is adjusted in 5 defined increments on the
brightness adjusting knob:
100% / 55% / 30% / 17% / 10%

5.2.3 Infinity port

If the microscope is equipped with an infinity port, in addi-
tion to the rear output, there is another access to the fluo-
rescence axis on the left/right side of the stand.
The infinity port is equipped with a mirror or a prism.

Infinity port with mirror

t
5IF
JOmOJUZ
QPSU
TMJEF
CBS
PO
UIF
SJHIUMFGU
TJEF
PG
UIF
TUBOE

guides 100% of the light to the rear output (mirror swung
out) or 100% of the light to the left/right output (mirror
swung in).

Infinity port with prism

t
5IF
JOmOJUZ
QPSU
TMJEF
CBS
PO
UIF
SJHIUMFGU
TJEF
PG
UIF
TUBOE

guides 100% of the light to the rear output (prism swung
out) or 50% of the light each to the rear and left/right out-
put (mirror swung in).

44
5. OPERATION - ILLUMINATION

5.2.4 Shutter
Leica DMi8 with manual shutter

t
0QFO
BOE
DMPTF
UIF
NBOVBM
TIVUUFS
VTJOH
UIF
TXJUDI
SPE

on the manually integrated fluorescence axis or using the
switch rod on the manual external fluorescence axis.

Manual integrated fluorescence axis

Manual external fluorescence axis

Leica DMi8 with motorized shutter

t
0QFO
PS
DMPTF
UIF
TIVUUFS
VTJOH
UIF
TIVUUFS
LFZ
PO
UIF
MFGU

side of the stand.
This switches the active illumination axis (TL or Fluor) on
or off.
When the shutter is open, the LED is illuminated.

t
"MUFSOBUJWFMZ
UIF
TIVUUFS
DBO
CF
PQFSBUFE
VTJOH
CVUUPOT

on the Touch Screen.

Gray = Shutter closed

Yellow = Shutter open

45
5. OPERATION - OBJECTIVES

5.3 Objectives

! Caution:
If necessary, first lower the focus to prevent damaging the
objective front lenses!

5.3.1 Changing objectives

Leica DMi8 with manual objective nosepiece

t
$POmHVSF
BO
PCKFDUJWF
CZ
SPUBUJOH
UIF
PCKFDUJWF
OPTF-

piece on the knurled disk.
During the manual objective change, be sure that the
nosepiece locks into place.

Leica DMi8 with manually encoded objective nosepiece

a b
t
$POmHVSF
BO
PCKFDUJWF
CZ
SPUBUJOH
UIF
PCKFDUJWF
OPTF-
piece on the knurled disk.
During the manual objective change, be sure that the
nosepiece locks into place.
The LED assigned to the selected objective on the front
side of the microscope is illuminated.

a Keypad for encoded objective nosepiece

b Keypad for encoded objective nosepiece and coded
fluo turret
Leica DMi8 with motorized objective nosepiece and front a b
operating panel with function keys

t
$POmHVSF
BO
PCKFDUJWF
CZ
QSFTTJOH
UIF
DPSSFTQPOEJOH
PC-
jective changer button on the keypad of the front side of
the microscope.
The LED of the objective changer button is illuminated.

a Keypad for motorized objective nosepiece

b Keypad for motorized nosepiece and motorized fluo tur-
ret

46
5. OPERATION - OBJECTIVES

Leica DMi8 - automated variants

The objectives can be swung into the beam path by mo-

torized action using variable function keys on the stand or
Leica SmartMove or using the Touch Screen.

Note:
The positions of the objectives in the objective turret have
been specified at the factory and must be observed when Function keys on the right side of microscope
installing the objectives.
(See Installation Manual).

When selecting an objective, the microscope automatically

selects:

t
5IF
NPTU
SFDFOUMZ
DPOmHVSFE
QPTJUJPO
PG
UIF
JMMVNJOBUFE

field diaphragm
t
5IF
NPTU
SFDFOUMZ
DPOmHVSFE
QPTJUJPO
PG
UIF
BQFSUVSF
EJB-
phragm

t
5IF
NPTU
SFDFOUMZ
DPOmHVSFE
MJHIU
JOUFOTJUZ
JO
UIF
SFTQFD-
tive contrast method (with DIC fine adjustment where ap-
plicable)
t
5IF
NPTU
SFDFOUMZ
DPOmHVSFE
GPDVT
JODMVEJOH
QBSGPDBM-
ity)

Adjusting the objectives using the Touch Screen:

t

6TF
UIF
tab to switch to the Magnification menu.

The information field on the top edge of the screen shows

the current total magnification at the specified observation
port.

Pressing the key allows you to switch between de-

tails for the various observation outputs.

The objective keys are labeled with the corresponding ob-

jective magnification. The objectives permitted for the se-
lected contrast method are identified with a white triangle.
Immersion objectives are also marked with drop symbol.

t
5P
TXJUDI
CFUXFFO
objectives, press the corresponding

key, which is then color-coded.

47
5. OPERATION - OBJECTIVES

Immersion objectives
t
'PS
immersion objectives use the appropriate immersion
medium.
OIL: Use optical immersion oil in accordance with DIN/
ISO standards
only
Cleaning oS. 89
W: Water immersion
IMM: Universal objective for water, glycerin,
Oil immersion

Caution!
Follow safety data sheet for immersion oil!

t
4FMFDU
BO
JNNFSTJPO
PCKFDUJWF
The objective nosepiece is lowered to the lower thres-
hold. This makes it possible to apply the immersion fluid
when switching from the dry objective to the immersion
objective. Conversely, the immersion fluid can be re-
moved.
The current objective remains in the beam path.

t
1SFTT
UIF
LFZ
GPS
UIF
EFTJSFE
JNNFSTJPO
PCKFDUJWF
XIJDI

is now approached.

Note:
For lockable immersion objectives, press the front part
upwards as far as it will go (approx. 2 mm). Then, after a
gentle turning motion to the right, the objective is locked.

For objectives with a correction mount, adapt the objec-

tive to the thickness of the cover slip by turning the knurled
knob.

Note:
If objectives are retrofitted, they have to be taught in using
the LAS X software: Afterwards, the parfocality should also
be taught in again.
See the instructions for the LAS X.

48
5. OPERATION - TUBE

5.4 Tube
There are also Leica DMi8 without tube.

Note:
Close any unused tube openings, as otherwise stray light
can interfere with observation.

Adjusting the interpupillary distance

t
"EKVTU
UIF
WJFXJOH
EJTUBODF
PG
UIF
FZFQJFDF
UVCFT
TP
UIBU

a congruent total image is seen.

Adjusting the viewing angle

t
'PS
UIF
FSHP
UVCFT
UIF
WJFXJOH
BOHMF
DBO
CF
BEKVTUFE

from 30° to 45° by tilting the binocular eyepiece.

Beam splitting in photo tubes

The light division is set manually by pulling out a control bar.

Observation(VIS) Photo
100 % 0%
0% 100 %
Alternatively: 50% 50%
BL Activating the Bertrand lens*

Switch rod

Bertrand lens centering

49
5. OPERATION - EYEPIECES

5.5 Eyepieces

Note:
The eyepiece's aperture protector must be removed or
folded back during microscopy while wearing eyeglasses. Protection screen
We recommend removing bifocals and spectacles with pro-
gressive-addition lenses when using the microscope. Adjusting the eyelens

t
'PS
UIF
BEKVTUBCMF
UVCFT
XJUI
EPDVNFOUBUJPO
PVUQVU

choose the 100% VIS position.

Eyepieces with inlaid graticule

t
'PDVT
UIF
HSBUJDVMF
CZ
BEKVTUJOH
UIF
FZFMFOT
JO
UIF
FZF-
piece.

t
'PDVT
PO
UIF
PCKFDU
UISPVHI
UIJT
FZFQJFDF

t
5IFO
DMPTF
UIBU
FZF
BOE
GPDVT
PO
UIF
PCKFDU
CZ
BEKVTUJOH
UIF

second ocular only.

Correction for vision problems

t
8JUI
ZPVS
SJHIU
FZF
MPPL
UISPVHI
UIF
SJHIU
FZFQJFDF
BOE

bring the specimen into sharp focus.

t
5IFO
XJUI
ZPVS
MFGU
FZF
WJFX
UIF
TBNF
QPTJUJPO
PG
UIF

specimen and rotate the left eyepiece tube until this posi-
tion is brought into sharp focus. While doing so, do not
change the adjustment of the z-position!

Note:
We recommend using the LAS X software to teach in eye-
pieces that are not included in the scope of delivery or are
retrofitted. This ensures that the total magnification speci-
fied on the Touch Screen is correct.
See the instructions for the LAS X.

50
5. OPERATION - STAGES AND OBJECT DISPLACEMENT

5.6 Stages and object displacement

A wide variety of stages is available in variants with fixed
stage, manual and motorized 3-plate cross-stage and scan-
ning stage with various mechanical stages and inserts.

The "Live on Stage" brochure provides an overview of the

accessories for live cell microscopy including the various
specimen stages, specimen holders and inserts.

You can download the brochure from

http://www.leica-microsystems.com/products/light-micro-
scopes/accessories/environmental-equipment-for-invert-
ed-microscopes/.

5.6.1 Object displacement

Fixed stage
The object displacement is carried out manually by moving
the specimen on the specimen stage or using the object
guide.

Manual 3-plate cross-stage

The object displacement in the x- and y-direction takes
place using a coaxial drive.

Motorized 3-plate cross-stage

Object displacement in the x- and y-direction can be per-
formed by the Leica SmartMove control element, the Leica
STP8000 main switch board or LAS X software. Move in x-direction

Scanning stage/linear motorized stage

Also read the separately provided instruction manual.

Note:
When using other external stages, observe the respective
instructions for the external stages.

WARNING
Move in y-direction
In spite of the low speed, there is a risk of pinching and
crushing injuries when moving the scanning stage.
Never put objects or body parts in the travel path!
Stage movement using Leica SmartMove

51
5. OPERATION - STAGES AND OBJECT DISPLACEMENT

5.6.2 Adjusting the step sizes on motorized stages

t
0O
UIF
5PVDI
4DSFFO
VTF
UIF
key to switch to

stage and focus controls. The X/Y coordinates of the cur-
rent stage position are displayed.

t
4FMFDU
UIF
Stage menu using the key.

t
:PV
DBO
DIBOHF

UIF
travel speed of the stage by

switching between the Coarse and Fine step sizes.
If the Coarse value is selected, the travel speed is the
same for all objectives.
The Fine value is adapted to the respective objective.

5.6.3 Saving and moving to stage positions

In the LAS X software, the programmed working area is Working area restriction
marked by a dashed line frame.
Current
stage position
You can reset the working area to the maximum stage 1
travel range via the Limits key.
2 Stored
3
stage position

Zero point

52
5. OPERATION - STAGES AND OBJECT DISPLACEMENT

Up to 5 stage positions and one load position (L) can be

stored.

t
4FMFDUJOH
TUPSFE
QPTJUJPOT
VTJOH
UIF
+ and – keys

t
4BWJOH
UIF
DVSSFOU
TUBHF
QPTJUJPO
VOEFS
UIJT
OVNCFS
VTJOH

the key

If a stage position was previously saved in this location, it

will be overwritten.

t
.PWJOH
UP
UIF
DVSSFOU
TUPSFE
QPTJUJPO
VTJOH
UIF
key
When driving to the load position, the objective nose-
piece is raised to the lower threshold.

Note:
If a stored position is outside the travel range of the stage,
for example as a result of subsequent changes to the travel
range in the LAS software, you must restore the maximum

range of the stage via Limits

to reach the position.

The stored positions will be saved until the microscope is

switched off and are deleted after a restart.

53
5. OPERATION - FOCUSING

5.7 Focusing

Note:
If your microscope is equipped with a closed loop, this can
be switched on and off using the LAS X software.
See the instructions for the LAS X.

5.7.1 Focusing the image

Leica DMi8 manual focus

t
'PDVT
UIF
JNBHF
CZ
UVSOJOH
UIF
GPDVT
EJBMT
PO
UIF
left and
right sides of the stand.
Coarse focusing

Fine focusing

Leica DMi8 with motorized focus/closed loop

t
'PDVT
UIF
JNBHF
VTJOH
UIF
SFBS
SPUBSZ
LOPC
PO
UIF
Leica Focusing
SmartMove control element.

t
"MUFSOBUJWFMZ
UIF
focus dials on the left and right sides of

the stand can also be used.

Note:
For coarse and fine focusing with the handwheel, 2 focus
stages each can be selected using the LAS X software.

54
5. OPERATION - FOCUSING

5.7.2 Focus thresholds

Leica DMi8 with motorized focus/closed loop

Note:
4
The parfocality is taught in at the factory. As a result of
screwing in the objectives during assembly, it might be nec-
essary to teach in the parfocality again. 3
We recommend checking the parfocality before setting the
thresholds and, if necessary, teaching them in again using 2
the LAS X software. 1

Note:
Right side of Leica DMi8 with motorized focus
The focus position and stops are stored by the microscope 1 Focusing
and retained from one session to the next when power is 2 Focus stop
switched off. (the LED illuminates when focus threshold is set)
3 Setting the focus position and focus threshold
Setting thresholds 4 Focus position
(the LED illuminates when the focus position is set)
Lower focus threshold:
t
1SFTT
UIF
SET key and the key

p
— Z
When the threshold is set, the LED is illuminated.
Pressing the same key combination again deletes the
threshold.
Moving to the thresholds
The lower threshold is the same for all objectives and can- Lower focus threshold:
not be exceeded. t
1SFTT
BOE
IPME
UIF
LFZ

Focus position: p
— Z
t
1SFTT
UIF
SET key and the key
— Focus position:
n Z Press and hold the key
—
When the focus position is set, the LED is illuminated. n Z
Pressing the same key combination again deletes the fo-
cus position.
Note:
The focus position cannot be exceeded. When moving to the thresholds, the keys must be held until
It should be defined for the dry objective with the highest the position has been reached.
magnification level. For all other objectives, it is set auto-
matically, taking into account the parfocality adjustment
and the working distance.

55
5. OPERATION - FOCUSING

Setting the focus via the Touch Screen

t
0O
UIF
5PVDI
4DSFFO
VTF
UIF
key for switching to

stage and focus controls.

t
4FMFDU
UIF
Focus Drive menu using the key.

The current Z position is shown in the information field.

t
8IFO
UIF
focus position is set, it can approached directly

using the key.

When the lower threshold is set, use the key to

reach the lower threshold.
The Z drive continues only as long as the button is held
depressed. The process can be interrupted at any time to
prevent a collision of the objective and specimen in case
of an incorrectly set lower stop.

t
5P
TFU
PS
DMFBS
UIF
GPDVT
QPTJUJPO
PS
UIF
MPXFS
threshold,
select the corresponding position under Set/Delete, then
press the Set or Delete key.

Focus position Lower stop

5.7.3 Adjusting the step sizes

Leica DMi8 with motorized focus/closed loop

t
6TF
Coarse and Fine to switch between coarse and fine

focusing at the stand handwheel or the focus knob of the
Leica SmartMove as applicable.

The Fine value is adapted to the respective objective. The

values are predefined logically. You can change the alloca-
tion using the LAS X software.
If the Coarse value is selected, the travel speed is the same
for all objectives. Coarse corresponds to the maximum Note:
speed. The allocation of a certain step size to an objective applies
not only for the z-drive, but also for defining the stage step
size, which is allocated to this objective after selecting Fine.

56
5. OPERATION - FOCUSING

5.7.4 AFC (Adaptive Focus Control)

Leica DMi8 with motorized focus/closed loop

The AFC can be used to actively hold a defined focus posi-

tion and automatically readjust this position regularly. This
is useful if, for example, a temperature change is caused by
opening the climate chamber during living cell experiments
at 37°C.
The AFC is activated, then the specimen on the stand is
focused using the handwheel and the stopping position is
saved.

t
"'$
NPOJUPSJOH
DBO
CF
QFSGPSNFE
VTJOH
WBSJBCMF
GVODUJPO

keys, LAS X software or via the Touch Screen.

Setting the AFC via the Touch Screen

t
0O
UIF
5PVDI
4DSFFO
VTF
UIF
key for switching to

stage and focus controls.

t
4FMFDU
UIF
Adaptive Focus Control menu using the

key.

t
"DUJWBUF
UIF
"'$
VTJOH
UIF
On/Off key. The knob behind
AFC turns green.

t
'PDVT
UIF
TQFDJNFO
BOE
TBWF
UIF
QPTJUJPO
VTJOH
UIF
Hold
key.

t
*G
UIF
QPTJUJPO
JT
IFME
UIF
LOPC
CFIJOE
Position will turn

green.

57
5. OPERATION - SELECTING THE PORT

5.8 Selecting the ports

t
5IF
top port can be operated manually using the switch
rod (see Tube Operation).

Leica DMi8 with manual or manually encoded ports

The manual switch rod on the right side of the stand en-
ables or disables the left side port.
VIS LEFT
100 % 0%
20% 80 %
Alternatively: 0% 100%

Leica DMi8 with motorized ports

Setting the ports via the Touch Screen:

t

6TF
UIF
key to switch to the Magnification menu.

t
4FMFDU
UIF
SJHIU
PS
MFGU
TJEF
QPSU
VTJOH
UIF
DPSSFTQPOEJOH

key or point 100% of the light at the eyepiece.
100% VIS Right side port

Left side port Bottom port

Note:
The "Select the bottom port" function can be assigned
to one of the variable function keys on the stand or Leica
SmartMove.

Note:
Side ports not in use must by covered with cover caps.

58
5. OPERATION - MAGNIFICATION CHANGER

5.9 Magnification Changer

Leica DMi8 with manually encoded Magnification Changer

As an option, a mechanical Magnification Changer can be

used.
Magnification factors: 1x; 1.6x

t
6TJOH
UIF
TMJEF
CBS
UPHHMF
CFUXFFO
Y
BOE
UIF
NBHOJmDB-
tion factor.

Note:
The mechanical Magnification Changer works on the eye-
piece and the top port.

Leica DMi8 with motorized Magnification Changer

As an option, a motorized Magnification Changer with 2 or 3

switch positions can be used.
Magnification factors:
For 3 switch positions: 1x ; 1.6x; 2x
For 2 switch positions: 1x ; 1.6x

Setting the Magnification Changer via the Touch Screen:

t
6TF
UIF
key to switch to the Magnification menu.

t
4FMFDU
UIF
NBHOJmDBUJPO
GBDUPS
VTJOH
UIF
DPSSFTQPOEJOH

key.

Note:
The selected factor is included in the calculation of the to-
tal magnification.

Note:
The motorized Magnification Changer works on all ports.

59
5. OPERATION - APERTURE AND ILLUMINATED FIELD DIAPHRAGM

5.10 Aperture diaphragm and field diaphragm

Leica DMi8 with manual aperture diaphragm Leica DMi8 with a fixed Transmitted Light arm

For a tiltable Transmitted Light arm:

t

5IF
NBOVBM
BQFSUVSF
EJBQISBHN
JT
BEKVTUFE
PO
UIF
DPO-
denser.

Manual aperture diaphragm

For a fixed Transmitted Light arm:
t

5IF
NBOVBM
BQFSUVSF
EJBQISBHN
JT
BEKVTUFE
PO
UIF
BSN

Leica DMi8 with manual illuminated field diaphragm Leica DMi8 with tiltable Transmitted Light arm

For a tiltable Transmitted Light arm:

t

5IF
NBOVBM
JMMVNJOBUFE
mFME
EJBQISBHN
JT
BEKVTUFE
PO
UIF

arm.

Note:
Manual or motorized illumi-
The illuminated field diaphragm is available only in conjunc- nated field diaphragm
tion with ≤ S28 condensers.

Manual aperture diaphragm

For an integrated fluorescence axis: Leica DMi8 with manual integrated fluorescence axis
t
5IF
NBOVBM
JMMVNJOBUFE
mFME
EJBQISBHN
JT
BEKVTUFE
VTJOH

a slide bar on the right side of the microscope.

Manual illuminated field dia-

phragm

60
5. OPERATION - APERTURE AND ILLUMINATED FIELD DIAPHRAGM

Leica DMi8 with motorized aperture and illuminated field

diaphragm

Both diaphragms have been set to suitable values for the

current objective and contrast method at the factory.

t
6TJOH
UIF
Aperture (aperture diaphragm) or Field (field

diaphragm) keys, the motorized diaphragms can be
changed at any time. The display on the Touch Screen
changes accordingly.

Note:
The old values will be overwritten by the current ones!

Note:
When using PH or DF, the aperture diaphragm is open all
the way and cannot be closed.

Setting the diaphragms via the Touch Screen

t
0O
UIF
5PVDI
4DSFFO
VTF
UIF
key for basic micro-

scope settings.

t
4FMFDU
UIF
Status/Illumination menu using the key.

t
"EKVTU
UIF
BQFSUVSF
BOE
mFME
EJBQISBHNT
VTJOH
UIF
+ and
– keys.

61
5. OPERATION - FILTER

5.11 Filter Leica DMi8 with tiltable Transmitted Light arm: Transmitted
Light filter
5.11.1 Transmitted Light filter
For a tiltable Transmitted Light arm:
t
5IF
5SBOTNJUUFE
-JHIU
mMUFS
BOE
UIF
EBSL
TUPQ
BSF
TXVOH

in and out on the right side of the Transmitted Light arm.

Note:
The front position is assigned at the factory using a shutter
(dark stop), which is interchangeable with a filter.

For a fixed Transmitted Light arm: Leica DMi8 with a fixed Transmitted Light arm: Transmitted
t
*OTFSU
UIF
mMUFS
JOUP
UIF
mMUFS
SFDFQUBDMF
PO
UIF
5SBOTNJU- Light filter
ted Light arm.

62
5. OPERATION - FILTER

5.11.2 Fluorescence Cubes

Note:
Observe the Installation Manual when equipping the fluo
turret or replacing individual filter cubes.

Leica DMi8 with manual fluorescent turret disk

t
5IF
filter cubes are swiveled manually into the beam path

by turning the fluorescent turret disk.
Color mark Cover
t
"
DPMPS
NBSL
PO
UIF
FEHF
PG
UIF
EJTL
JOEJDBUFT
UIF
DVS- Knurled ring for setting the filter cube
rent filter cube. If the shipped configuration is changed by
inserting another filter cube, note the color of the position
where the filter cube was inserted so you can allocate it
later.

Changing filter cubes:

t

3FNPWF
UIF
DPWFS
GSPN
UIF
nVPSFTDFOU
UVSSFU
EJTL
5IF

cover is secured in place by a magnetic holder and can
be detached from the front.

t
1VMM
UIF
mMUFS
DVCF
GPSXBSE
UP
SFNPWF
JU
BOE
TMJEF
UIF
OFX

filter cube straight into the receptacle.

t
1VU
UIF
DPWFS
CBDL
JO
QMBDF

t
6TJOH
B
NPUPSJ[FE
nVPSFTDFODF
UVSSFU
EJTL
XJUI
-*%
-FJDB

Radio Frequency Identification Device), there is an auto-
matic full rotation of the turret to read out the filter. For
additional instructions, see the chapter entitled “Leica Empty position for mounting a filter cube. The filter cube is pushed in
RFID Module” o S. 65 from the front evenly until it clicks into place.

Leica DMi8 with manually encoded fluorescent turret disk

For encoded fluorescence For motorized fluorescent
t
5IF
mMUFS
DVCFT
BSF
TXJWFMFE
NBOVBMMZ
JOUP
UIF
CFBN
QBUI
nosepiece disk turret disk
by turning the fluorescent turret disk.
The LED allocated to the filter cube and located on the
front side of the microscope is illuminated.

Leica DMi8 with motorized fluorescent turret disk and front

operating panel with function keys

t
$POmHVSF
B
mMUFS
DVCF
CZ
QSFTTJOH
UIF
DPSSFTQPOEJOH
mM-
ter cube changer button on the keypad of the front side of
the microscope.
The LED of the filter cube changer button is illuminated.

63
5. OPERATION - FILTER

Leica DMi8 - motorized variants

Operating the fluorescence via Touch Screen

t
0O
UIF
5PVDI
4DSFFO
VTF
UIF
key to configure the

contrast method.

t
4FMFDU
FLUO.

t
5IF
BWBJMBCMF
filter cubes are displayed. Select the de-

sired cube using the corresponding key.

Changing filter cubes o S. 63

Change filter cubes for laser applications

WARNING
For use of the microscope with lasers, Leica
Microsystems CMS GmbH offers special microscope
variants with additional safety features.Laser couplings
require corresponding safety devices that have to be in-
spected and installed by trained personnel.

t
5IF
cover of the fluorescent turret disk is secured

using 2
DIN 912 M4x6 lens head Allen screws.
(Order No. 11 703 121 630 000)
Detach both screws.

WARNING
Store the screws in a safe place. Never operate the
microscope when the cover is not screwed into place!

t Change the filter cubes as described for the manual Leica

DMi8.

t
$MPTF
BOE
SFGBTUFO
UIF
DPWFS

64
5. OPERATION - MEMORY FUNCTION ON THE TOUCHSCREEN

5.11.3 Leica RFID Module 5.11.3.4Ports

5.11.3.1Product Description
This microscope contains the RFID module „LID Module
1.0“.

It recognizes the used filter cubes in the fluoturret.

The different filter cubes are equipped with corresponding
passive tags (Type D6.7 special).
During initialization phase of the turret, these tags are read I2C-Port
by the LID Module 1.0.
5.11.3.5FCC / IC Compliance
The tags cannot be written or modified by the LID Module
1.0.

Note:
This equipment has been tested and found to comply with
the limits for a Class A digital device, pursuant to Part 15
of the FCC Rules. These limits are designed to provide rea-
sonable protection against harmful interference when the
equipment is operated in a commercial environment. This
equipment generates, uses, and can radiate radio frequen-
cy energy and, if not installed and used in accordance with
LID Module 1.0 the instruction manual, may cause harmful interference to
radio communications. Operation of this equipment in a res-
5.11.3.2Specifications LID Module 1.0
idential area is likely to cause harmful interference in which
Supply Voltage 5V case the user will be required to correct the interference at
Dimensions 36 x 25 x 9.8 mm his own expense.
RFID Protocol
Transmit frequncy
ISO 15693
13,56 MHz
! Notice:
This device complies with Part 15 of the FCC Rules [and
Operating temperature -5°C to +65°C
with Industry Canada licence-exempt RSS standard(s)].
Storage temperature -20°C to +85°C Operation is subject to the following two conditions:
Communication interface I2C 1. this device may not cause harmful interference, and
2. this device must accept any interference received, in-
5.11.3.3Specifications RFID Tag D6.7 special cluding interference that may cause undesired opera-
tion.
Modulation Scheme Close coupling
Dimensions D=6,7mm ! Notice:
TH=2,6mm Changes or modifications made to this equipment not ex-
RFID Protocol ISO 15693 pressly approved by Leica Microsystems CMS GmbH may
Transmit frequncy 13,56 MHz void the FCC authorization to operate this equipment.
Read write type 2kbit EEProm
Hereby, Leica Microsystmems CMS GmbH declares
Operating temperature -15°C to +65°C
that the radio equipment type LID Module 1.0 designa-
Storage temperature -25°C to +125°C tion of type of radio equipment is in compliance with Di-
rective 2014/53/EU. The full text of the EU declaration of
conformity is available at the following internet address:
www.leica-microsystems.com

65
5. OPERATION - TRANSMITTED LIGHT CONTRAST METHOD

5.12 The memory function on the Touch Screen CAUTION

Up to 6 user-specific settings can be saved via the Touch Do not look into the eyepieces when toggling the con-
Screen. In doing so, combinations of contrast method and trast method!
objective are stored. One exception is fluorescence mode. During the toggling procedure, the maximum
In this mode, the combination of fluorescence filter and ob- radiant power of the light source may be present on the
jective is stored. eyepieces for a short time and can temporarily blind the
user!

t
0O
UIF
5PVDI
4DSFFO
VTF
UIF
key to switch to ba- Leica DMi8 with manual Transmitted Light method
sic microscope settings.
The contrast method on the manual or encoded Leica DMi8
can be operated using the manual condenser, the manual
t
4FMFDU
UIF
Memory Function menu using the key. objective nosepiece, or the rotary knobs and slide bars on
the stand.

Teaching in a combination t
*G
OFDFTTBSZ
mSTU
UPHHMF
UP
UIF
Transmitted Light axis (TL)

by opening the shutter.
t
4FU
UIF
EFTJSFE
DPNCJOBUJPO (Slide bar on the manual external or integrated fluores-
t
1SFTT
UIF
LFZ
UP
CF
BMMPDBUFE cence axis or using the shutter key for the motorized
t
5IFO
QSFTT
UIF
Set key. shutter.)
Deleting a saved combination
t
1SFTT
UIF
DPSSFTQPOEJOH
LFZ Leica DMi8 with motorized Transmitted Light method
t
5IFO
QSFTT
UIF
Delete key.
All contrast methods on the automated Leica DMi8 can be
Setting a saved combination selected and operated via the Touch Screen, the variable
function keys or the LAS X software. The only exceptions
t
1SFTT
UIF
DPSSFTQPOEJOH
LFZ
are methods involving components that have to be oper-
t
'JOBMMZ
QSFTT
Set.
ated manually (e.g. systems with manual analyzers). The
following describes operation of the automated Leica DMi8
5.13 Transmitted Light contrast method
via the Touch Screen. Refer to the separate instructions for
operation via the software.

t

*G
OFDFTTBSZ
TXJUDI
UP
UIF
5SBOTNJUUFE
-JHIU
BYJT
VTJOH

the TL/Fluor key (TL).

t
0O
UIF
5PVDI
4DSFFO
VTF
UIF
key to configure the

contrast method.

t
4FMFDU
UIF
EFTJSFE
5SBOTNJUUFE
-JHIU
DPOUSBTU
NFUIPE

66
5. OPERATION - TRANSMITTED LIGHT CONTRAST METHOD

Note: Shutter control

If all positions of the fluorescent turret disk have been as-
signed, the "DAPI" filter cube can be replaced with the "DA-
PI-TL" filter cube using LAS X software. Transmitted Light
contrast methods are possible with this filter cube.

5.13.1 Brightfield (TL)

Leica DMi8 with manual Transmitted Light method Toggling
Transmitted Light/fluorescence
t
*G
OFDFTTBSZ
TFU
UIF
5-
#SJHIUmFME
QPTJUJPO
PS
BO
FNQUZ

position on the fluo turret, or select the "DAPI-TL" filter
cube.

For a tiltable Transmitted Light arm:

t

4FMFDU
UIF
#SJHIUmFME
QPTJUJPO
PO
UIF
DPOEFOTFS
BF.

For a fixed Transmitted Light arm:

t
'PS
DPSSFDU
IFJHIU
BEKVTUNFOU
PG
UIF
4
BOE

S40/0.45 condensers, the stand has marks (1). These
marks pertain to a liquid height of 15 mm. For stands
with double marks, the upper and lower mark repre-
sent a range with different liquid heights. Move the
condenser holder while observing the specimen to at-
tain an optimal image.

Note:
For the stands with 3-plate mechanical stages, posi-
tion the arm 25 mm below the marks, as the illumina-
tion axis is installed 25 mm higher on an adapter than
on the fixed stage.

t
1SFTT
UIF
TUPQ
MFWFS


BOE
BEKVTU
UIF
5SBOTNJUUFE

Light illumination arm until the upper edge of the car-
rier is even with the corresponding condenser height
mark.

t
4XJOH
BMM
SFNBJOJOH
PQUJDBM
DPNQPOFOUT
TVDI
BT
UIF
BOB-
lyzer, polarizer or IC prisms, from the beam path.

t

*OTFSU
B
5SBOTNJUUFE
-JHIU
TQFDJNFO

t

4FMFDU
ZPVS
PCKFDUJWF

67
5. OPERATION - TRANSMITTED LIGHT CONTRAST METHOD

t

'PDVT
UIF
JNBHF
VTJOH
UIF
GPDVT
EJBMT

t

$POmHVSF
UIF
CSJHIUOFTT
PO
UIF
JOUFOTJUZ
SPUBSZ
LOPC

t
'PS
BO
PQUJNBM
mFME
EJBQISBHN
TFUUJOH
DIFDL
UIF
,PFIMFS

illumination.

Leica DMi8 with motorized Transmitted Light method

t
4FMFDU
UIF
DPOUSBTU
NFUIPE
BF (Brightfield).

The Brightfield position is approached for the motorized Condenser setting for tiltable Transmitted Light arm - con-
condenser. figuring BF position
An empty position or the filter cube "DAPI-TL" on the fluo-
rescent turret disk is approached automatically.

t
*OTFSU
B
5SBOTNJUUFE
-JHIU
TQFDJNFO

t
4FMFDU
B
TVJUBCMF
PCKFDUJWF
JO
UIF
NBHOJmDBUJPO
NFOV
3
t
'PDVT
UIF
JNBHF
VTJOH
UIF
SPUBSZ
LOPC
PO
UIF
-FJDB

SmartMove or using the focus dial. 2

t

$POmHVSF
UIF
CSJHIUOFTT
PO
UIF
JOUFOTJUZ
SPUBSZ
LOPC
1

Note:
1
You can find additional information regarding the correct
settings for Koehler illumination in Leica Science Lab.
Link: Condenser setting for fixed Transmitted Light arm
http://www.leica-microsystems.com/science-lab/ 1 Marks
2 Stop lever for condenser adjustment
3 Aperture diaphragm

68
5. OPERATION - TRANSMITTED LIGHT CONTRAST METHOD

5.13.2 Phase Contrast (TL)

(Integrated Phase Contrast, see 5.13.6)
Leica DMi8 with manual Transmitted Light method

t
*G
OFDFTTBSZ
TFU
UIF
5-
#SJHIUmFME
QPTJUJPO
PS
BO
FNQUZ

position on the fluo turret, or select the "DAPI-TL" filter
cube.

t

4FMFDU
B
1IBTF
$POUSBTU
PCKFDUJWF
0CKFDUJWFT
UIBU
BSF

suitable for Phase Contrast are engraved with PH.

For a tiltable Transmitted Light arm:

t

4FMFDU
UIF
DPSSFTQPOEJOH
light ring on the condenser.

For a fixed Transmitted Light arm:

t
$POmHVSF
UIF
DPOEFOTFS
IFJHIU
BOE
SFNPWF
UIF
*.$
Condenser setting for tiltable Transmitted Light arm - con-
slit diaphragm slider, if necessary. figuring light ring, e.g. PH1

t
(VJEF
UIF
1IBTF
$POUSBTU light ring slide with the

marking "TOP LEFT" on the top left laterally into the re-
ceptacle until it clicks into place.
(Caution: there are various slide bars for the condens- Light ring slide
ers!)

t
6TF
UIF
MJHIU
SJOH
PG
UIF
MJHIU
SJOH
TMJEF
UIBU
DPSSFTQPOET

to the magnification (5, 10/20 or 40).

Note:
The Phase Contrast light ring slide is encoded. As soon as
the slider is taken out of the Brightfield position (available
opening) and engaged in a Phase Contrast position (any
light ring), the brightness increases. Conversely, switch-
ing from Phase Contrast to Brightfield decreases the
brightness. Phase Contrast for fixed Transmitted Light arm

6 1 6 2

4
1 Slider for light rings (S80/0.30) 5
2 Slider for light rings (S40/0.45) 4
3 Click stop 4

4 Light rings
5 Brightfield position 3

6 "TOP LEFT" marking Light ring slide for fixed Transmitted Light arm

69
5. OPERATION - TRANSMITTED LIGHT CONTRAST METHOD

t

$PNQMFUFMZ
PQFO
UIF
BQFSUVSF
PO
UIF
DPOEFOTFS

t
4XJOH
BMM
SFNBJOJOH
PQUJDBM
DPNQPOFOUT
TVDI
BT
UIF
BOB-
lyzer, polarizer or IC prisms, from the beam path.

t

*OTFSU
B
1IBTF
$POUSBTU
TQFDJNFO

t

'PDVT
UIF
JNBHF
VTJOH
UIF
GPDVT
EJBMT

t

$POmHVSF
UIF
CSJHIUOFTT
PO
UIF
JOUFOTJUZ
SPUBSZ
LOPC

Leica DMi8 with motorized Transmitted Light method

t
4FMFDU
UIF
PH contrast method (Phase Contrast).

The correct light ring on the motorized condenser is

swung inwards.

t
*OTFSU
B
5SBOTNJUUFE
-JHIU
TQFDJNFO

t
4FMFDU
B
TVJUBCMF
PCKFDUJWF
JO
UIF
NBHOJmDBUJPO
NFOV
Objectives that are suitable for Phase Contrast are en-
graved with PH.

t
'PDVT
UIF
JNBHF
VTJOH
UIF
SPUBSZ
LOPC
PO
UIF
-FJDB

SmartMove or using the focus dial.

t

$POmHVSF
UIF
CSJHIUOFTT
PO
UIF
JOUFOTJUZ
SPUBSZ
LOPC

Note:
When selecting the Phase Contrast method, the aperture
diaphragm is opened fully and can not be adjusted.

70
5. OPERATION - TRANSMITTED LIGHT CONTRAST METHOD

5.13.3 Darkfield (TL)

Leica DMi8 with manual Transmitted Light method

t
*G
OFDFTTBSZ
TFU
UIF
5-
#SJHIUmFME
QPTJUJPO
PS
BO
FNQUZ

position on the fluo turret, or select the "DAPI-TL" filter
cube.

t

4FMFDU
B
%BSLmFME
PCKFDUJWF

t

4FMFDU
UIF
DPSSFTQPOEJOH
%BSLmFME
TUPQ
MJHIU
SJOH

PO
UIF

condenser.

t

$PNQMFUFMZ
PQFO
UIF
BQFSUVSF
PO
UIF
DPOEFOTFS

t
4XJOH
BMM
SFNBJOJOH
PQUJDBM
DPNQPOFOUT
TVDI
BT
UIF
BOB-
lyzer, polarizer or IC prisms, from the beam path.

t

*OTFSU
B
%BSLmFME
TQFDJNFO

t

'PDVT
UIF
JNBHF
VTJOH
UIF
GPDVT
EJBMT
Condenser setting for tiltable Transmitted Light arm - con-
t

$POmHVSF
UIF
CSJHIUOFTT
PO
UIF
JOUFOTJUZ
SPUBSZ
LOPC figuring Darkfield stop (light ring)

Leica DMi8 with motorized Transmitted Light method

t
4FMFDU
UIF
DPOUSBTU
NFUIPE
DF (Darkfield).

The Darkfield ring (dark stop) is swung in for the motor-

ized condenser.

t
*OTFSU
B
5SBOTNJUUFE
-JHIU
TQFDJNFO

t
4FMFDU
B
TVJUBCMF
PCKFDUJWF
JO
UIF
NBHOJmDBUJPO
NFOV

t
'PDVT
UIF
JNBHF
VTJOH
UIF
SPUBSZ
LOPC
PO
UIF
-FJDB

SmartMove or using the focus dial.

t

$POmHVSF
UIF
CSJHIUOFTT
PO
UIF
JOUFOTJUZ
SPUBSZ
LOPC

Note: Note:
The maximum applicable objective aperture for the Dark- When the Darkfield method is selected, the aperture dia-
field is for the condenser S1 0.70 and for the condenser S23/ phragm is opened fully and cannot be adjusted.
S28/S40 0.40.

71
5. OPERATION - TRANSMITTED LIGHT CONTRAST METHOD

5.13.4 Polarization (TL)

Leica DMi8 with manual Transmitted Light method

t
*G
OFDFTTBSZ
TFU
UIF
5-
#SJHIUmFME
QPTJUJPO
PS
BO
FNQUZ

position on the fluo turret, or select the "DAPI-TL" filter
cube.

t

4FMFDU
UIF
#SJHIUmFME
QPTJUJPO
PO
UIF
DPOEFOTFS Swinging in polarizer

t
4XJOH
BMM
*$
QSJTNT
PVU
PG
UIF
CFBN
QBUI

t

4FMFDU
ZPVS
PCKFDUJWF

t
4XJOH
UIF
polarizer on the condenser into the beam path.

t
*OTFSU
UIF
analyzer on the right side of the stand up to the

click stop.

t
#SJOH
UIF
QPMBSJ[FS
BOE
BOBMZ[FS
JOUP
DSPTT
QPTJUJPO
VOUJM
Condenser setting for tiltable Transmitted Light arm - con-

they reach maximum darkness. figuring BF position

t
*OTFSU
B
TQFDJNFO

t

'PDVT
UIF
JNBHF
VTJOH
UIF
GPDVT
EJBMT

t

$POmHVSF
UIF
CSJHIUOFTT
PO
UIF
JOUFOTJUZ
SPUBSZ
LOPC

Pushing in analyzer

72
5. OPERATION - TRANSMITTED LIGHT CONTRAST METHOD

Leica DMi8 with motorized Transmitted Light method

t
4FMFDU
UIF
POL (Polarization) contrast method

Motor procedure:
t
"GUFS
UIF
POL contrast method has been selected, the
polarizer is swung in (if the microscope is equipped with
these components). The analyzer cube is also automati-
cally brought into the beam path.

Combined procedure:
t
:PV
BMTP
IBWF
UIF
PQUJPO
PG
DPNCJOJOH
QVSFMZ
NFDIBOJDBM

and motorized components, i.e. you can combine a me-
chanical analyzer and a motorized polarizer.

Manual procedure:
For a manual swing polarizer and a mechanical analyzer,
proceed with the manual Transmitted Light method as de-
scribed for the Leica DMi8.

t
4FMFDU
B
TVJUBCMF
PCKFDUJWF
JO
UIF
NBHOJmDBUJPO
NFOV

t
*OTFSU
B
TQFDJNFO

t
'PDVT
UIF
JNBHF
VTJOH
UIF
SPUBSZ
LOPC
PO
UIF
-FJDB

SmartMove or using the focus dial.

t

$POmHVSF
UIF
CSJHIUOFTT
PO
UIF
JOUFOTJUZ
SPUBSZ
LOPC

73
5. OPERATION - TRANSMITTED LIGHT CONTRAST METHOD

5.13.5 Differential Interference Contrast – DIC (TL)

Leica DMi8 with manual Transmitted Light method

t
*G
OFDFTTBSZ
TFU
UIF
5-
#SJHIUmFME
QPTJUJPO
PS
BO
FNQUZ

position on the fluo turret, or select the "DAPI-TL" filter
cube.

t

4FMFDU
BO
PCKFDUJWF

t

4FMFDU
UIF
DPSSFTQPOEJOH
8PMMBTUPO
QSJTN
DPOEFOTFS
PO
Swinging in polarizer

the condenser.

t
4FMFDU
UIF
DPSSFTQPOEJOH
8PMMBTUPO
QSJTN
PCKFDUJWF
PO

the objective nosepiece.

t
4XJOH
UIF
polarizer on the condenser into the beam path.

t
5IFO
JOTFSU
UIF
analyzer on the right side of the stand un-

til it locks into place.

t
*OTFSU
B
TQFDJNFO
Condenser setting for tiltable Transmitted Light arm - con-
t

'PDVT
UIF
JNBHF
VTJOH
UIF
GPDVT
EJBMT
figuring Wollaston prism
t

$POmHVSF
UIF
CSJHIUOFTT
PO
UIF
JOUFOTJUZ
SPUBSZ
LOPC

t
'PS
mOF
BEKVTUNFOU
VTF
UIF
BEKVTUJOH
TDSFX
CFMPX
UIF

objective nosepiece.

Setting objective prism

Fine adjusting the
objective prism

74
5. OPERATION - TRANSMITTED LIGHT CONTRAST METHOD

Leica DMi8 with motorized Transmitted Light method

t
4FMFDU
UIF
DIC (Differential Interference Contrast) con-

trast method.

t
5IF
QPMBSJ[FS
MPDBUFE
JO
UIF
DPOEFOTFS
BOE
UIF
mUUJOH
DPO-
denser prisms are automatically brought into the beam
path. The corresponding objective prism and the analyzer
cube are also approached automatically.

t

1MBDF
B
%*$
TQFDJNFO
PO
UIF
TUBHF

t
4FMFDU
B
TVJUBCMF
PCKFDUJWF
JO
UIF
NBHOJmDBUJPO
NFOV

t
'PDVT
UIF
JNBHF
VTJOH
UIF
SPUBSZ
LOPC
PO
UIF
-FJDB

SmartMove or using the focus dial.

t

$POmHVSF
UIF
CSJHIUOFTT
PO
UIF
JOUFOTJUZ
SPUBSZ
LOPC

t
5IF
BEKVTUNFOU
JT
DBSSJFE
PVU
WJB
UIF
CVUUPOT
+ and – on
the Touch Screen.

Manual alternative:
t
.BOVBMMZ
TXJOH
UIF
QPMBSJ[FS
PO
UIF
DPOEFOTFS
JOUP
UIF

beam path.

t
5IFO
BMTP
NBOVBMMZ
JOTFSU
UIF
BOBMZ[FS
PO
UIF
SJHIU
TJEF

of the stand until it locks into place.

Guide the objective and condenser prisms manually until

a correct combination is displayed in the Touch Screen.

t
'PS
mOF
BEKVTUNFOU
VTF
UIF
LOVSMFE
SJOH
CFMPX
UIF
PCKFD-
tive nosepiece.

Fine adjusting the objective prism

75
5. OPERATION - TRANSMITTED LIGHT CONTRAST METHOD

5.13.6 Integrated Phase Contrast – IPH (TL)

Note:
The Integrated Phase Contrast requires a condenser on the
tiltable Transmitted Light arm.

Leica DMi8 with manual Transmitted Light method

t
*G
OFDFTTBSZ
TFU
UIF
5-
#SJHIUmFME
QPTJUJPO
PS
BO
FNQUZ

position on the fluo turret, or select the "DAPI-TL" filter
cube.

t
4FMFDU
B
#SJHIUmFME
PCKFDUJWF
XJUI
FZFQPJOU
#
PS
$
Receptacle for Phase Contrast module
t

4FMFDU
UIF
DPSSFTQPOEJOH
light ring on the condenser (see
table). Phase Contrast module for tiltable Transmitted Light illumi-
nation carrier
t

$PNQMFUFMZ
PQFO
UIF
BQFSUVSF
PO
UIF
DPOEFOTFS 1 Centering screws for light ring 20x - 63x
2 Position for objectives 20x - 63x
t
4XJOH
BMM
SFNBJOJOH
PQUJDBM
DPNQPOFOUT
TVDI
BT
UIF
BOB-
lyzer, polarizer or IC prisms, from the beam path. 3 Brightfield position
4 Position for objectives 5x - 10x
t
*G
OFDFTTBSZ
SFNPWF
UIF
DPWFS
PG
UIF
IPMEFS
BOE
JOTFSU

the Phase Contrast module evenly from the front on the 5 Phase ring 20x - 63x
left side of the microscope. 6 Phase ring 5x - 10x

t
4MJEF
UIF
1IBTF
$POUSBTU
NPEVMF
JOUP
UIF
DPSSFDU
FZFQPJOU
7 Fastening screw for light ring 20x - 63x

B or C. 6 5 4 3 2 1

t

*OTFSU
B
1IBTF
$POUSBTU
TQFDJNFO

t

'PDVT
UIF
JNBHF
VTJOH
UIF
GPDVT
EJBMT

t

$POmHVSF
UIF
CSJHIUOFTT
PO
UIF
JOUFOTJUZ
SPUBSZ
LOPC

IP0 for 5x e.g. NPlan 5x Objective with eyepoint B

IP1 for 10x e.g. NPlan 10 x Objectives with eyepoint B and

for 20x e.g. NPlan L 20 x Objectives with eyepoint C

IP2 for 40x e.g. HCX PL FL L 40 x Objectives with eyepoint C

IP3 for 63x e.g. PL FL 63x/0.70 Objectives with eyepoint C

76
5. OPERATION - TRANSMITTED LIGHT CONTRAST METHOD

Leica DMi8 with motorized Transmitted Light method

t
4FMFDU
UIF
PH (Phase Contrast) contrast method.

The correct light ring on the motorized condenser is

swung inwards.

t
*OTFSU
B
5SBOTNJUUFE
-JHIU
TQFDJNFO

t
4FMFDU
B
TVJUBCMF
PCKFDUJWF
JO
UIF
NBHOJmDBUJPO
NFOV

(eyepoint B or C).

t
*G
OFDFTTBSZ
SFNPWF
UIF
DPWFS
PG
UIF
IPMEFS
BOE
JOTFSU

the Phase Contrast module evenly from the front on the
left side of the microscope.

t
4MJEF
UIF
1IBTF
$POUSBTU
NPEVMF
JOUP
UIF
DPSSFDU
FZFQPJOU

B or C.

t
'PDVT
UIF
JNBHF
VTJOH
UIF
SPUBSZ
LOPC
PO
UIF
-FJDB

SmartMove or using the focus dial.

Note:
When selecting the Phase Contrast method, the aperture
diaphragm is opened fully and can not be adjusted.

Note:
For centering the phase ring 20x-63x, refer to the Installa-
tion Manual.

77
5. OPERATION - TRANSMITTED LIGHT CONTRAST METHOD

5.13.7 Integrated Modulation Contrast – IMC (TL) IMC module for tiltable Transmitted Light arm
Leica DMi8 with manual Transmitted Light method 1 Position for objectives with eyepoint C
2 Position for objectives with eyepoint B
t
*G
OFDFTTBSZ
TFU
UIF
5-
#SJHIUmFME
QPTJUJPO
PS
BO
FNQUZ

position on the fluo turret, or select the "DAPI-TL" filter 3 Brightfield position
cube. 4 Height adjustment

t
4FMFDU
B
#SJHIUmFME
PCKFDUJWF
XJUI
eyepoint B or C. 5 Fine adjustment

3
t
4XJOH
BMM
SFNBJOJOH
PQUJDBM
DPNQPOFOUT
TVDI
BT
UIF
BOB- 2
1
lyzer or IC prisms from the beam path.

Inserting the IMC module:

t
*G
OFDFTTBSZ
SFNPWF
UIF
DPWFS
PG
UIF
IPMEFS
BOE
JOTFSU

the IMC module evenly from the front on the left side of 4
the microscope. 5

For a tiltable Transmitted Light arm: IMC module for fixed Transmitted Light arm
t
4MJEF
UIF
*.$
NPEVMF
JOUP
UIF
DPSSFDU
FZFQPJOU
#
PS
$

1 IMC module
For a fixed Transmitted Light arm: 2 IMC modulator
t
*OTFSU
UIF
*.$
NPEVMBUPS
JOUP
UIF
*.$
NPEVMF
GSPN
UIF

3 Fine adjustment
side according to the eyepoint B, C or D of the objec-
tive. 4 Positioning depending on eyepoint B, C or D
Ensure that the "TOP" marking faces upwards.
1
t
&OHBHF
UIF
TMJEF
JO
UIF
*.$
QPTJUJPO 2
(Push in for IMC, pull out for the Brightfield).

3
4

IMC module used for fixed Transmitted Light arm

78
5. OPERATION - TRANSMITTED LIGHT CONTRAST METHOD

Setting the IMC slit diaphragms:

For a tiltable Transmitted Light arm: Swinging in polarizer
t
0O
UIF
DPOEFOTFS
TFMFDU
UIF
DPSSFTQPOEJOH
TMJU
JMMVNJ-
nation for the current magnification.

t
4XJOH
UIF
polarizer on the condenser into the beam

path.

Note:
The position of the polarizer influences the appearance of
the relief. The best relief images are obtained in the extinc-
tion position; the relief disappears at a 90° position. Condenser setting for tiltable Transmitted Light arm - con-
figuring slit diaphragm
For a fixed Transmitted Light arm:
t
*G
OFDFTTBSZ
SFNPWF
UIF
Phase Contrast slider in the IMC slit diaphragm slider
Transmitted Light arm.

t
)PME
UIF
*.$
TMJEF
TP
UIBU
UIF
NBSLJOH
501
-&'5
JT
BU

the top left and the rest of the marking faces forwards.
The click stops are on the front longitudinal side of the
slide.
(Depending on whether the S40/0.45 or S80/0.30 con-
denser is used, various IMC slit diaphragm sliders ex-
ist).

t
*OTFSU
UIF
EJBQISBHN
TMJEF
GSPN
UIF
SJHIU
JOUP
UIF

Transmitted Light arm.

t
&OHBHF
UIF
TMJEF
JO
UIF
*.$
QPTJUJPO
5IF
SPVOE
PQFO-
ing is the Brightfield position.
1 "TOP LEFT" marking on the diaphragm slide
2 Click stops
Note:
3 IMC slit diaphragm slider for S80/0.30
The IMC slit diaphragm slider is encoded. As soon as
the slider is taken out of the Brightfield position (avail- 4 IMC slit diaphragm slider for S40/0.45
able opening) and engaged in the IMC position, the 5 Adjusting screw on the IMC slit diaphragm slider
brightness increases. Conversely, switching from IMC
to Brightfield decreases the brightness. 3
1
t
To adjust the slit width, adjust the slider of the IMC slit 2 5
2
diaphragm slider to the position allocated on the ob- 1
jective, e.g. to the 10x marking for the 10x objective.
5
2 2
4

79
5. OPERATION - TRANSMITTED LIGHT CONTRAST METHOD

t
*OTFSU
B
TQFDJNFO

t

'PDVT
UIF
JNBHF
VTJOH
UIF
GPDVT
EJBMT

t

$POmHVSF
UIF
CSJHIUOFTT
PO
UIF
JOUFOTJUZ
SPUBSZ
LOPC

Fine adjustment:
For a tiltable Transmitted Light arm: IMC module for tiltable Transmitted Light arm
t
5IF
mOF
BEKVTUNFOU
JT
NBEF
VTJOH
UIF
LOVSMFE
TDSFX

on the IMC modulator and the polarizer.

t
6TJOH
UIF
TMJEFS
ZPV
DBO
BMTP
PGGTFU
UIF
IFJHIU
UP
BE-
just the slit diaphragms to the focal plane. This setting
depends on the specimen being observed.

Note:
The position of the polarizer influences the appearance of
the relief. The best relief images are obtained in the extinc-
tion position; the relief disappears at a 90° position.

For a fixed Transmitted Light arm:

t
5IF fine adjustment occurs via the knurled screw on IMC module for fixed Transmitted Light arm
the IMC modulator.

Note:
For adjusting the aperture diaphragms, refer to the Installa-
tion Manual.

80
5. OPERATION - FLUORESCENCE

Leica DMi8 with motorized Transmitted Light method

t
$BVUJPO
'PS
UIF
*OUFHSBUFE
.PEVMBUJPO
$POUSBTU
mSTU
FO-
able the DIC button!

The correct slit diaphragm is configured and the polarizer

is automatically swung inwards on the motorized con-
denser.

t
*OTFSU
B
TQFDJNFO

t
4FMFDU
B
TVJUBCMF
PCKFDUJWF
JO
UIF
NBHOJmDBUJPO
NFOV

(eyepoint B or C).

t
*G
OFDFTTBSZ
SFNPWF
UIF
DPWFS
PG
UIF
IPMEFS
BOE
JOTFSU
IMC module for tiltable Transmitted Light arm
the IMC module evenly from the front on the left side of
1 Position for objectives with eyepoint C
the microscope.
2 Position for objectives with eyepoint B
t
4MJEF
UIF
*.$
NPEVMF
JOUP
UIF
DPSSFDU
eyepoint B or C. 3 Brightfield position

t
'PDVT
UIF
JNBHF
VTJOH
UIF
SPUBSZ
LOPC
PO
UIF
-FJDB
4 Height adjustment
SmartMove or using the focus dial. 5 Fine adjustment

t

$POmHVSF
UIF
CSJHIUOFTT
PO
UIF
JOUFOTJUZ
SPUBSZ
LOPC 3
2
1
t
5IF
fine adjustment is made using the knurled screw (5)
and the polarizer.

4
Note:
5
The position of the polarizer influences the appearance of
the relief. The best relief images are obtained in the extinc-
tion position; the relief disappears at a 90° position.

t
6TJOH
UIF
TMJEFS


ZPV
DBO
BMTP
PGGTFU
UIF
IFJHIU
UP
BE-
just the slit diaphragms to the focal plane. This setting de-
pends on the specimen being observed.

Note:
For adjusting the aperture diaphragms, refer to the Installa-
tion Manual.

81
5. OPERATION - TRANSMITTED LIGHT CONTRAST METHOD

5.14 Fluorescence Manual integrated fluorescence axis

1 Manual filter change
Note: 2 Analyzer
To replace filter cubes, refer to Chapter 5.11, Filters o. S. 3 Manual shutter
63
4 Manual setting for FIM
Leica DMi8 with manual fluorescence

t
*G
OFDFTTBSZ
mSTU
TXJUDI
PWFS
UP
UIF
fluorescence axis

(Fluor) by opening the shutter (pull out switch rod (3) for
10
manual shutter or push shutter button for motorized shut-
ter). 4

t
*G
OFDFTTBSZ
QVMM
PVU
UIF
BOBMZ[FS
TMJEFS


DPNQMFUFMZ
UP
3
open the beam path.

t
4FU
UIF
MJHIU
JOUFOTJUZ
PO
UIF
Leica EL6000 compact light 1 2

source.
Manual external fluorescence axis

Note: 3 Manual shutter

If the Leica DMi8 is equipped with a 2 port infinity port (oS. 44) 4 Manual setting for FIM
switch over to the current port .oS. 58 5 Connection for light guide
6 Diaphragm module
t
8IFO
VTJOH
UIF
Fluorescence Intensity Manager (FIM):
The brightness is adjusted in 5 defined increments on the 7 Receptacle for EFW or structured illumination
FIM slider (4).
100% / 55% / 30% / 17% / 10%

t
5IF
filter cubes are swiveled manually into the beam path

by turning the fluorescent turret disk (1).

t
"
DPMPS
NBSL
PO
UIF
FEHF
PG
UIF
EJTL
JOEJDBUFT
UIF
DVS-
rent filter cube. If the shipped configuration is changed by
inserting another filter cube, note the color of the position
where the filter cube was inserted so you can allocate it 5 4 6 7 3
later.

82
5. OPERATION - FLUORESCENCE

Leica DMi8 with manually encoded fluorescent turret disk

t
5IF
filter cubes are swiveled manually into the beam path

by turning the fluorescent turret disk. For encoded fluorescence For motorized fluorescent
The LED allocated to the filter cube and located on the nosepiece disk turret disk
front side of the microscope is illuminated.

Manually encoded Leica DMi8 with motorized fluorescent

turret disk

t
"EKVTU
B
mMUFS
DVCF
CZ
QSFTTJOH
UIF
DPSSFTQPOEJOH
filter

cube changer button on the keypad of the front side of the
microscope.
The LED of the filter cube changer button is illuminated.

Leica DMi8 with motorized fluorescence

t

*G
OFDFTTBSZ
TXJUDI
PWFS
UP
UIF
nVPSFTDFODF
BYJT
Fluor)

with the button TL/Fluor.

Operating the fluorescence via Touch Screen:

t

*G
OFDFTTBSZ
TXJUDI
PWFS
UP
UIF
nVPSFTDFODF
BYJT
'MVPS


with the IL button.

t
3PUBUF
PO
UIF
5PVDI
4DSFFO
WJB
UIF
CVUUPO
for con-

figuring the contrast method.

t
'PS
JODJEFOU
MJHIU
TFMFDU
UIF
DPOUSBTU
NFUIPE
FLUO.

t
*OTFSU
B
TQFDJNFO

t
4FMFDU
B
TVJUBCMF
PCKFDUJWF
JO
UIF
NBHOJmDBUJPO
NFOV

t
5IF
DVSSFOU
nVPSFTDFODF
mMUFS
DVCF
JT
EJTQMBZFE
PO
UIF

Touch Screen. If necessary, push the button for the de-
sired fluorescence filter cube.

83
5. OPERATION - FLUORESCENCE

t
:PV
NBZ
QSPUFDU
ZPVS
TQFDJNFO
GSPN
GBEJOH
CZ
DMPTJOH
UIF

incident light shutter.
To do so, push the IL shutter button.

t
'PDVT
UIF
JNBHF
VTJOH
UIF
SPUBSZ
LOPC
PO
UIF
-FJDB

SmartMove or using the focus dial.

t
4FU
UIF
MJHIU
JOUFOTJUZ
PO
UIF
Leica EL6000 compact light

source.
(See separate Instructions for Use).

t
8IFO
VTJOH
UIF
Fluorescence Intensity Manager (FIM):

The brightness is adjusted in 5 defined increments on the
brightness adjusting knob:
100% / 55% / 30% / 17% / 10%

or alternatively, on the Touch Screen via the button FIM

84
5. OPERATION - COMBINATION METHOD

5.15 Combination method

Leica DMi8 with motorized Transmitted Light method and
motorized fluorescence:

Depending on the configuration of your microscope, up to

two combination methods are possible:

FLUO/PH and FLUO/DIC

t
0O
UIF
5PVDI
4DSFFO
VTF
UIF
key to configure the

contrast method.

t
*OTFSU
B
TQFDJNFO

t
4FMFDU
B
TVJUBCMF
PCKFDUJWF
JO
UIF
NBHOJmDBUJPO
NFOV

t
'PS
UIF
DPNCJOBUJPO
TFMFDU
UIF
DPOUSBTU
NFUIPE
FLUO-PH
or Fluo-DIC.

FLUO-PH:
t

5IF
DVSSFOU
nVPSFTDFODF
mMUFS
DVCF
JT
EJTQMBZFE
PO
UIF

Touch Screen. If necessary, push the button for the de-
sired fluorescence filter cube.

t
5IF
JMMVNJOBUJPO
TFUUJOHT
GPS
UIF
nVPSFTDFODF
BOE
5SBOT-
mitted Light axis can be adjusted separately.
To do so, toggle between the using the TL/Fluor button.

FLUO-DIC:
t
4XJUDI
CFUXFFO
UIF
UXP
DPOUSBTU
NFUIPET
CZ
QSFTTJOH

the FLUO-DIC key.

t
*O
'-60
NPEF
UIF
DVSSFOU
nVPSFTDFODF
mMUFS
DVCF
JT
EJT-
played on the Touch Screen. If necessary, push the but-
ton for the desired fluorescence filter cube.

t
*O
%*$
NPEF
UIF
%*$
mOF
BEKVTUNFOU
JT
NBEF
VTJOH
UIF
+
and – key.

Note:
The manual analyzer must be used for the FLUO/DIC meth-
od.

85
6. TROUBLESHOOTING

6. Troubleshooting
Problem Cause/remedy

Stand
The microscope does not respond. Ensure that the AC outlet has power.
Make sure that the electronics box or the microscope is
connected to the power supply.
Check the cable connections.
Inform Service and have the supply unit fuse checked.

Illumination
The image is completely dark. Open the shutter.
Check the connection of the lamp housing to the micro-
scope (Transmitted Light).
Ensure that the lamps are not defective.
Check the connection of the EL6000 compact light source
(fluorescence).
Switch over to the active port.
Inform the service department and have them check
whether a fuse is defective.

The image is not uniformly illuminated. Remove all unneeded filters from the light path.

Brightfield
The specimen cannot be brought into focus.  Use the correct immersion medium.
Place the specimen with the cover slip downward onto
the stage.
Make sure that the thickness of the cover slip is correct
and corresponds to the specifications on the objective.
Make sure that you are using an objective with cover slip
correction.
Adjust the correction ring if present on the objective.

86
6. TROUBLESHOOTING

Problem Cause/remedy

Darkfield
No definite DF contrast is possible. Be sure that a DF objective is being used.
The objective aperture setting is too high:
Maximum 0.7 for condenser S1
Maximal 0.4 for condenser S23/28/40
If necessary, reduce the objective aperture using the iris
diaphragm on the objective.
Check the condenser centering.

The image is not uniformly illuminated. The magnification is too weak.

Use a higher magnification.
If necessary, remove the condenser lenses or the con-
denser head.

Unwanted stray light. Clean the specimen and neighboring lens surfaces.

Phase Contrast
Phase Contrast cannot be adjusted. The specimen is too thick, too thin or too brightly stained.
Refractive indexes of the mounting medium and specimen
are identical, so that there is no phase jump.
The cover glass is not placed uniformly.
Check that the correct light ring is positioned.
Check that the correct light slider is positioned.
The S40/0.45 condenser has been switched with the
S80/0.30 condenser.
Open the aperture diaphragm completely.
IMC modulator in IMC position.
Check the centering of the light rings.

Polarization
Polarization contrast cannot be adjusted. Bring the polarizer and analyzer into cross position until
they reach maximum darkness (without specimen).

87
6. TROUBLESHOOTING

Problem Cause/remedy

Transmitted-light interference contrast

No Transmitted Light interference contrast is possible. The specimen is too thick or too thin.
The mounting medium or specimen are of birefringent
material. Rotate the specimen.
The difference in the refractive indices of the specimen
and the mounting medium is too small.
The cover slip is too thick.
Check the Koehler illumination.
Bring the polarizer and analyzer into cross position until
they reach maximum darkness (without specimen).
Check whether the compatible condenser prism and the
corresponding objective prism are configured.
Check the correct placement of the IC condenser prisms.

Integrated Modulation Contrast

IMC cannot be adjusted. Check that the correct objective is positioned (eyepoint C
or D).
Check the position of the aperture diaphragm.
For a fixed Transmitted Light arm:
Check that the IMC modulator and the IMC slit diaphragm
slider are inserted correctly and engaged in the IMC posi-
tion.
For a tiltable Transmitted Light arm:
Check that the IMC module is inserted correctly and en-
gaged in the position compatible to the objective.
Check that the correct condenser (S40/0.45 or S80/0.30) is
positioned and that the condenser height is correct.
Switch off the fluorescence filter.
Open the aperture diaphragm completely.
IMC modulator in IMC position.

Fluorescence
The image is completely dark (no fluorescence). Open the shutter.
Check the antigen-antibody combination.
Switch over to the active port (or Infinity Port).

The fluorescence is too weak. Unsuitable specimen (stored incorrectly, too old,
bleached).
Unspecific filter combination.
Objective with too low of a numerical aperture.
Eyepiece magnification too high.
Room where microscope is located too bright.
Incorrect beamsplit on the trinocular tube.
Secondary light due to reflection at condenser.

88
7. CARE OF THE MICROSCOPE

7. Care of the Microscope

7.2 Cleaning
Caution!
Unplug the power supply before performing cleaning
! Caution:

and maintenance work! Residual fiber and dust can create unwanted background
Protect electrical components from moisture! fluorescence during fluorescence microscopy.

Cleaning coated parts

Microscopes in warm and warm-damp climatic zones re-
quire special care in order to prevent the build up of fungus. Dust and loose dirt particles can be removed with a soft
The microscope should be cleaned after each use, and the brush or lint-free cotton cloth.
microscope optics should be kept painstakingly clean.
Clinging dirt can be cleaned as necessary with a low-con-
centrated soap solution, petroleum ether or ethyl alcohol.
7.1 Dust Cover For cleaning coated parts, use a linen or leather cloth that
is moistened with one of these substances.

! Caution:
Note: Thinners containing acetone, xylene or nitrogen can
To protect against dust, cover the microscope and acces- harm the microscope and thus must not be used.
sories with the dust cover after each use.
Test cleaning solutions of unknown composition on a less
visible area of the unit first. Be sure that coated or plastic
surfaces do not become matted or etched.
CAUTION
Cleaning the specimen stage
Let the microscope and lamp housings cool down Remove light-colored spots on the stage by rubbing with
before covering the stand with a dust cover. The dust paraffin oil or acid-free Vaseline.
cover is not heat-resistant. In addition, condensation
may occur.

89
7. CARE OF THE MICROSCOPE

Cleaning glass surfaces and objectives Removing immersion oil

The cleaning of glass surfaces and objectives in particular
should be carried out exclusively as outlined in the bro-
Caution!
chure "Cleaning of Microscope Optics". The information can
be downloaded at: Follow safety notes for immersion oil!

h t t p : / / w w w. l e i c a - m i c r o s y s t e m s . c o m / p r o d u c t s / First, wipe off the immersion oil with a clean cotton cloth,
light-microscopes/life-science-research/inverted-micro- and then re-wipe the surface several times with ethyl alco-
scopes hol.

.
Select the microscope type and go to the "Download" page. 7.3 Handling acids and bases
For examinations using acids or other aggressive chemi-
In case of questions, please contact our Technical Service. cals, particular caution must be taken.

! Caution:
Never allow the optics and mechanical parts to come
into direct contact with these chemicals.

90
8. ESSENTIAL CONSUMABLE AND SPARE PARTS

8. Essential Consumable and Spare Parts

Order No.
Material No. Name Used for

Screw cap for unused objective receptacles

020-422-570-000 Screw cap M 25 Objective nosepiece

Cover for unoccupied objective-DIC-disk opening

11 090-144-020-088 DIC cover microscope stand

Dust and light stop for analyzer opening

11 020-437-101-013 Analyzer opening cover microscope stand

Dust and light stop for camera port openings

11 020-387-556-009 Analyzer opening cover microscope stand

Replacement eyecup (antiglare protection) for HC PLAN eyepiece

021-500-017-005 HC PLAN eyecup 10x/25 eyepiece
021-264-520-018 C PLAN eyecup 10x/22 eyepiece
021-264-520-018 HC PLAN eyecup 10x/20 eyepiece

Immersion oil in accordance with DIN/ISO standards, fluorescence-free

11 513 859 Type F, ISO 8036, OIL and IMM objectives
Very low self-fluorescence and oil condenser heads
Expressly recommended for
fluorescence applications and
all APO objectives, 10 ml
11 513 860 Type N, ISO 8036,
Low self-fluorescence, 20 ml
11 513 861 Type N, ISO 8036,
Low self-fluorescence, 250 ml

Screws for the fluorescence nosepiece cover

11 703 121 630 000 DIN 912 Allen screw Cover of the fluo turret
M4x6 lens head disk for laser microscope systems

Additional spare parts such as cover caps, stickers, screws, dust covers etc., on request.

91
9. APPENDIX – ASSIGNING THE VARIABLE FUNCTION KEYS

9. Appendix
9.1 Assigning the variable function keys
The variable function keys can always be defined by the
user via the LAS X software.

Button 2
Button 1
Depending on the configuration, useful functions are al-
ready assigned to the function key upon delivery.
Button 4
Button 3

Preassigning the function keys:

Biological applications Biological applications

Microscope with Magnification Changer: Microscope without Magnification Changer:
Button 1 TL Button 1 Switch through objectives to the right
Button 2 Photo Button 2 Photo
Button 3 Toggle Magnification Changer Button 3 Switch through objectives to the left
Toggle bottom port (if present) Toggle bottom port (if present)
Button 4 Button 4
Otherwise: Port switchover Otherwise: Port switchover

Industry applications Industry applications

Microscope with Magnification Changer: Microscope without Magnification Changer:
Button 1 TL Button 1 Switch through objectives to the right
Button 2 Photo Button 2 Photo
Button 3 Toggle Magnification Changer Button 3 Switch through objectives to the left
Button 4 Toggle ports Button 4 Toggle ports

92
9. APPENDIX – ASSIGNING THE VARIABLE FUNCTION KEYS

Possible assignments of the variable function keys to the stand and SmartMove:

Function button Function

BF Brightfield Transmitted Light
PH Phase Contrast Transmitted Light
ICT Interference contrast, Transmitted Light
DF Darkfield Transmitted Light
POL Polarization Transmitted Light
CHANGE TL Switches through all Transmitted Light contrast methods
INT n Increases the brightness (Transmitted Light)
INT p Reduces the brightness (Transmitted Light)
AP n Opens aperture diaphragm (Transmitted Light)
AP p Closes the aperture diaphragm (Transmitted Light)
FD n  Opens the field diaphragm (Transmitted Light)
FD p Closes the field diaphragm (Transmitted Light)
SHT TL Opens/closes the Transmitted Light shutter
CHG FLT Switch TL filter
FLUO Fluorescence (last filter cube)
CUBE 1-6 Chooses the fluorescence cube at position 1-6
CUBE Switches through the fluorescence cube clockwise (1 o6)
CUBE Switches through the fluorescence cube counterclockwise (6 o1)
CHG FLUO Switches through all fluorescence cubes
INT FLUO n Increases the brightness (fluorescence)
INT FLUO p Reduces the brightness (fluorescence)
FD FLUO n Opens the field diaphragm (fluorescence)
FD FLUO p Closes the field diaphragm (fluorescence)
CHG FW Toggles the filter wheel functions
IFW Activates the internal filter wheel
SHT FL Opens/closes the fluo shutter
ExMan Activates the excitation manager
FIM Activates the fluorescence intensity manager
COMBI Combination method (PH fluorescence or DIC fluorescence)
CHG CB Switches through all combination methods
CHG OBJ Switches through objectives clockwise
CHG OBJ Switches through objectives counterclockwise
DRY/IMM Switch dry/immersion
CHG Mag.-Chg. Switches through the Magnification Changer clockwise
XY PRECISE Activate stage precisely
1x Tube lens Activate lens 1x
Z COARSE Enable coarse focus
XY PRECISE Activate stage precisely
XY FAST Activate stage quickly
Bottom P Bottom port on/off
AFC ON/OFF Switches AFC on/off
AFC HOLD Holds the current position
Acquire Image/Photo Capture an image with the camera

Additional abbreviations for objective names, filter name, magnifications.

93
9. APPENDIX – ABBREVIATION GLOSSARY

9.2 Abbreviation glossary

AFC Adaptive Focus Control

AP Aperture diaphragm
BF Brightfield
COMBI Combination methods
CUBE Fluorescence cube
DF Darkfield incident / Transmitted Light
DIC Differential Interference Contrast
ExMan Excitation manager
FD Field diaphragm
FIM Fluorescence intensity manager
FLUO/Fluor Fluorescence axis (incident light)
ICR Interference contrast, incident light
ICT Interference contrast, Transmitted Light
IFW Internal filter wheel
IL Incident light
INT Intensity
IMC Integrated Modulation Contrast
IPH Integrated Phase Contrast
PH Phase Contrast
POL Polarization, incident / Transmitted Light
TL Transmitted Light
SET Set focus position or lower threshold
SHT Shutter
—
n Z
Focus position
p
— Z
Lower threshold

94
9. APPENDIX – OVERVIEW OF TOUCHSCREEN MENUS

9.3 Overview of Touch Screen pictograms

Navigation bar Observation ports

Basic microscope settings 100% eyepiece

Contrast method 100% camera

Magnification Eyepiece/camera splitting

Stage and focus controls

100% to lower, left or right port
Touch screen settings
Top port: eyepiece/camera splitting
Menus

Lower port/left or right port split

Status/illumination

Memory function
Left or right port split/top port with further split-
ting
Focus operation
Top port/rear port split
Stage operation
Control
AFC (Adaptive Focus Control)

Z-drive Toggle button

Saving
Focus position
Delete
Lower threshold For the table: reset working area to the maxi-
mum positioning range of the stage
Objectives
Reload
Valid dry objective For the table: move the current stored position
Valid immersion objective
Various
Shutter
Touch Screen calibration
Incident-light shutter open

Transmitted-light shutter closed Call up the version number

95
9. APPENDIX – ADDITIONAL INFORMATION

9.4 Additional information

General microscopy Objectives
Refer to the Leica Science Lab For information on the objectives such as the objective
classes and the marking of objectives, refer to
http://www.leica-microsystems.com/science-lab/
http://www.leica-microsystems.com/de/produkte/objektive/
to find additional useful information and instructions on the
microscope methods.
Accessories
Under the category "Basics in Microscopy", you can find, The "Live on Stage" brochure provides an overview of the
for instance: accessories for live cell microscopy including the various
specimen stages, specimen holders and inserts.
t
5IF
JOTUSVDUJPOT
GPS
TFUUJOH
UIF
DPSSFDU
Koehler illumina-
tion You can download the brochure from
http://www.leica-microsystems.com/de/produkte/Lichtmi-
t
5IF
QSPDFEVSF
GPS
DPOUSBTU
NFUIPET
TVDI
BT kroskope/Zubehör/Inkubationssysteme für Leica inverse
Phase Contrast Mikroskope.
Modulation contrast
Differential Interference Contrast

t
'MVPSFTDFODF
UFDIOJRVFT

t
5IF
JOTUSVDUJPOT
GPS
DMFBOJOH
UIF
NJDSPTDPQF
PQUJDT

and much more.

96