Name _________________________ Period _______

Date ________________
Regents Biology

ENZYME REVIEW

1. An enzyme and four different molecules are shown in the diagram below.

Enzyme

Molecules: 0 (>
(A) (B) (C) ( D)

The enzyme would most likely affect reactions involving

(1) molecule A, only

@molecule C, only

(3) molecules Band 0

(4) molecules A and C

2. Base your answers to questions 2 through 4 on the diagram below that represents a human
enzyme and four types of molecules present in a solution in a flask.

Enzyme Molecules

o
A B c 0

Which molecule would most likely react with the enzyme?

3. Explain your answer to question 2. What principle about how enzymes work does the question
illustrate?

L Oc..~ ~ '?~ ':M e, .e \1\""f.., '( Wl~'S 0. C.:h V e Si±( 1.-10.0 0­

S ~ee t'\Ac.vt YV'~ .1 $u \.? {t'l' a U 1>.

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Name __________________________
Regents Biology

4. Match the enzymes with their substrates and functions.

'6 A. amylase 1. synthesizes DNA

~ B. protease 2. digests sugar in beer (maltose)

-6- c. lactase 3. digests starch (amylose)

-.L D. DNA polymerase 4. synthesizes ATP

'2- E. maltase 5. digests milk sugar (lactose)

~ F. ATP synthase 6. digests proteins

5. Base your answers to the following questions on the graph below and on your knowledge of
biology .

Pepsin Trypsin
(digests (dIgests
proteinlO protein in
stomach) Intestine)

o 1 2 3 4 5 6 7 8 9 10 11 12 13 14

pH

6 . What is the optimal pH for pepsin? _-=3=--___________________________

7. Is this pH acid or basic? ___ __G-\__
CA 'a_'_U
______________
_
8. In what organ of the digestive system does pepsin work? S-roVv'O\CV1
9. What is the optimal pH fortrypSin? __ e,'-'~ _____________________
10. In what organ of the digestive system does trypsin work?
.
11. Is this pH acid or basic? ----..:'alLA~S:>-ll~U~-----------------
12. Neither enzyme works at a pHs of --.J\w2~\~\--=:~--',_\_4~____________

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Name _____________________ Regents Biology

13. An incomplete graph is shown below. What two internal body conditions could appropriately
be used to replace letter Z on the axis?

Effect of Z on Enzyme Activity

z
pH 1-e~pe~tt1VV0
14. What kind of organic molecule is an enzyme? .;::p,-,V\)~-,-tc.....:::..-·,"---'\1''---_ _ _ _ _ _ _ __
15. List 2 internal environmental factors that affect how well enzymes function.

y~ -~~~-p~---------
16. What happens to water when you heat it to 100°C?

\\ \00'\ s
17. What happens to proteins dissolved in that water when you heat it to 100°C?

Th~'t \? e cOVY\ e, clt V'CtN r (d

18. What specific change happens to an enzyme that stops it from working when you heat it?

19. Explain why changing the shape of an enzyme could affect the ability of the enzyme to
function.

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 Name _____________________ Regents Biology

20. Draw a generalized graph of the action of an enzyme from the human body as the
temperature changes from O°C to 100°C. Mark the temperature of optimal enzyme activity.

/ '\
/ 1\
II \
J \
/ 1
1
II
I ,
\
J 1\
/ '~
o ,0 20 30 40 ~ bO -10 00 "tu '00 ....-\
((yau V'~ ) ..-u-W\{? 0 c ("0\\ h· '1J
21. What most likely happens to the rate of reaction of a human enzyme when the temperature
is increased gradually from 10°C to 30°C. Explain your answer.

\rI\D V1 V1~

yY\Oy~ \ i l4.tL\A to \V\tc..Va.ct.

\
22. What most likely happens to the rate of reaction of a human enzyme when the temperature
is increased gradually from 40°C to 90°C. Explain your answer.

23. What is the optimal temperature for the functionality of a human enzyme? ___________

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