BIOINFORMATICA

APOC4

Grupa M1524
Pricop Ana

Chisinau, 2019
 Exercițiul 1. Asocierea dintre gena de interes și Factor de risk

GENE APOC4 – apolipoprotein C4

Homo sapiens (human)

 Processed peptides: Apolipoprotein C-IV

 Also known as: APO-CIV, APOC-IV
 GeneID: 346
 This gene encodes a lipid-binding protein belonging to the apolipoprotein gene family.
The protein is thought to play a role in lipid metabolism. Polymorphisms in this gene may
influence circulating lipid levels and may be associated with coronary artery disease risk. This
gene is present in a cluster with other related apolipoprotein genes on chromosome 19. Naturally
occurring read-through transcription exists between this gene and the neighboring downstream
apolipoprotein C-II (APOC2) gene.
 Tabel 1. Rezultatele căutării informațiilor pe portalul NCBI
Database category Database name Database description Number of records
Literature PubMedCentral Full-text journal articles 167

NLM Catalog Books,journals and more 11222

in the NLM Collections
PubMed Scientific and medical 8
abstracts or citations
Bookshelf Bools and reports 1
Genes Gene Collected information 1
about gene localization
GEO profiles Gene expression and 15
molecular abundance
profiles
Proteins Structure Protein sequences 419
Genomes BioProject Biological projects 711
providing data to NCBI
SRA High-throughput DNA and 162
RNA sequence read
archive
Genetics dbGaP Genotype and 2
phenotypeinteraction
studies
dbVar Genome structural 1
variations studies
MedGen Medical genetics literature 63
and links

Rezultatele căutării informațiilor pe portalul PubMedCentral

1: ImmGen report: sexual dimorphism in the immune system transcriptome
Shani Talia Gal-Oz, Barbara Maier, Hideyuki Yoshida, Kumba Seddu, Nitzan Elbaz, Charles
Czysz, Or Zuk, Barbara E. Stranger, Hadas Ner-Gaon, Tal Shay
Nat Commun. 2019; 10: 4295. Published online 2019 Sep 20. doi:В 10.1038/s41467-019-
12348-6
PMCID: PMC6754408

2: Possible epigenetic regulatory effect of dysregulated circular RNAs in Alzheimer’s

disease model
Woo-Jin Lee, Jangsup Moon, Daejong Jeon, Yong-Won Shin, Jung-Suk Yoo, Dong-Kyu Park,
Soon-Tae Lee, Keun-Hwa Jung, Kyung-Il Park, Ki-Young Jung, Manho Kim, Sang Kun Lee,
Kon Chu
Sci Rep. 2019; 9: 11956. Published online 2019 Aug 16. doi:В 10.1038/s41598-019-48471-z
PMCID: PMC6697677

3: Plasma ApoE elevations are associated with NAFLD: The PREVEND Study
Eline H. van den Berg, James P. Corsetti, Stephan J. L. Bakker, Robin P. F. Dullaart
PLoS One. 2019; 14(8): e0220659. Published online 2019 Aug 6.
doi:В 10.1371/journal.pone.0220659
PMCID: PMC6684074

4: Gene-based analysis in HRC imputed genome wide association data identifies three novel
genes for Alzheimer’s disease
Emily Baker, Rebecca Sims, Ganna Leonenko, Aura Frizzati, Janet C. Harwood, Detelina
Grozeva, GERAD/PERADES, CHARGE, ADGC, EADI, IGAP consortia, Kevin Morgan, Peter
Passmore, Clive Holmes, John Powell, Carol Brayne, Michael Gill, Simon Mead, Paola
BossГ№, Gianfranco Spalletta, Alison M. Goate, Carlos Cruchaga, Wolfgang Maier, Reinhard
Heun, Frank Jessen, Oliver Peters, Martin Dichgans, Lutz FröLich, Alfredo Ramirez, Lesley
Jones, John Hardy, Dobril Ivanov, Matthew Hill, Peter Holmans, Nicholas D. Allen, B. Paul
Morgan, Sudha Seshadri, Gerard D. Schellenberg, Philippe Amouyel, Julie Williams, Valentina
Escott-Price
PLoS One. 2019; 14(7): e0218111. Published online 2019 Jul 8.
doi:В 10.1371/journal.pone.0218111
PMCID: PMC6613773

5: Multi-Omics Analysis Reveals Up-Regulation of APR Signaling, LXR/RXR and FXR/RXR

Activation Pathways in Holstein Dairy Cows Exposed to High-Altitude Hypoxia
Zhiwei Kong, Chuanshe Zhou, Liang Chen, Ao Ren, Dongjie Zhang, Zhuzha Basang, Zhiliang
Tan, Jinhe Kang, Bin Li
Animals (Basel) 2019 Jul; 9(7): 406. Published online 2019 Jul 1. doi:В 10.3390/ani9070406
PMCID: PMC6680605

6: Proteomic profiling of plasma biomarkers in acute ischemic stroke due to large vessel
occlusion
Chuan Qin, Xin-Ling Zhao, Xiao-Tong Ma, Luo-Qi Zhou, Long-jun Wu, Ke Shang, Wei Wang,
Dai-Shi Tian
J Transl Med. 2019; 17: 214. Published online 2019 Jul 1. doi:В 10.1186/s12967-019-1962-8
PMCID: PMC6604304

7: FunSPU: A versatile and adaptive multiple functional annotation-based association test of

whole-genome sequencing data
Yiding Ma, Peng Wei
PLoS Genet. 2019 Apr; 15(4): e1008081. Published online 2019 Apr 29.
doi:В 10.1371/journal.pgen.1008081
PMCID: PMC6508749

8: Plasma Protein and MicroRNA Biomarkers of Insulin Resistance: A Network-Based

Integrative -Omics Analysis
Hyungwon Choi, Hiromi W. L. Koh, Lihan Zhou, He Cheng, Tze Ping Loh, Ehsan Parvaresh
Rizi, Sue Anne Toh, Gabriele V. Ronnett, Bevan E. Huang, Chin Meng Khoo
Front Physiol. 2019; 10: 379. Published online 2019 Apr 5. doi:В 10.3389/fphys.2019.00379
PMCID: PMC6460474
9: FoxK1 and FoxK2 in insulin regulation of cellular and mitochondrial metabolism
Masaji Sakaguchi, Weikang Cai, Chih-Hao Wang, Carly T. Cederquist, Marcos Damasio, Erica
P. Homan, Thiago Batista, Alfred K. Ramirez, Manoj K. Gupta, Martin Steger, Nicolai J. Wewer
Albrechtsen, Shailendra Kumar Singh, Eiichi Araki, Matthias Mann, Sven Enerbäck, C. Ronald
Kahn
Nat Commun. 2019; 10: 1582. Published online 2019 Apr 5. doi:В 10.1038/s41467-019-09418-
0
PMCID: PMC6450906

10: Analysis of 1508 Plasma Samples by Capillary-Flow Data-Independent Acquisition Profiles

Proteomics of Weight Loss and Maintenance
Roland Bruderer, Jan Muntel, Sebastian MГјller, Oliver M. Bernhardt, Tejas Gandhi, Ornella
Cominetti, Charlotte Macron, JГ©rГґme Carayol, Oliver Rinner, Arne Astrup, Wim H.M. Saris,
Jörg Hager, Armand Valsesia, Loïc Dayon, Lukas Reiter
Mol Cell Proteomics. 2019 Jun; 18(6): 1242–1254. Published online 2019 Apr 4.
doi:В 10.1074/mcp.RA118.001288
PMCID: PMC6553938

Gene Clinical Signifacance

ZNF 296 Pathogenic
 Exercițiul 2. Date structurale

Official symbol-APOC4.Official Full Name-apolipoprotein C4.Primary source-

HGNC:HGNC:611.Gene type-protein coding.Organism-Homo sapiens.Lineage- Eukaryota;
Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires;
Primates; Haplorrhini; Catarrhini; Hominidae; Homo.Also known as-APO-CIV or APOC-IV.
This gene encodes a lipid-binding protein belonging to the apolipoprotein gene family. The
protein is thought to play a role in lipid metabolism. Polymorphisms in this gene may influence
circulating lipid levels and may be associated with coronary artery disease risk. This gene is
present in a cluster with other related apolipoprotein genes on chromosome 19. Naturally
occurring read-through transcription exists between this gene and the neighboring downstream
apolipoprotein C-II (APOC2) gene.
Restricted expression toward liver.
Location:19q13.32

Homo sapiens apolipoprotein C2 (APOC2), RefSeqGene on chromosome 19

NCBI Reference Sequence: NG_008837.1
GenBank Graphics
>NG_008837.1 Homo sapiens apolipoprotein C2 (APOC2), RefSeqGene on chromosome 19
TTGCCCAGGTCACACGGTGAAGAAGTGAGGGATTCGAAGCCCAGGTCCATCTGAAG
CCAGAGTCACCCAG
AGGAGAAAGAGTTGGAATTGAGAACTCAAGGAATGCTTGGAAGTGATCGGGCTCGA
GCCCACCTAGGAAG
AAACAGAGGCTGGAGACATGAGACTGTGTTGCTATTTCCTCTCATCAACCCTTGGGC
CCTATTGAGGCCC
TACCACAAGCCTGGCCCTGCAGCCCAGTGACTAGGAGAAATTAGACACAAGATAAT
AATAACAGCAATGA
TCTTTTTTTTTTTTCTGAGACGGAGTCTTGCTCTTTCGCCCAGGCTGGACTGCAGTGG
CGCGATCTCGGC
TCAATGCAAGCTCCACCTCCCAGGTTCACGCCATTCTCCTGCCTCAGCCTCCCGAGT
AGCTAGGACTACA
GGCGCCTGCCACCACGCCTGGCTAATTTTTCATATTTTTAGTAGAGATGGGGTTTCA
CCGTGTTAGCCAA
GATGGTCTCAATCTCCTGACCTCGTGATCCGCCTGCCTCGGCCTCCCAAAGTGTTGG
GGTTACAGGCATG
AGCCACCGCGCCTGGCCAACAGCAATGATCTTTGAGCACCTATATTGCCAGTCTCCA
CGGTAAGAGCTTT
CTTCATTTTTTGTTTTGTTTTGTTTCAAGACAGAGTCTTGCTCTGTCACCCAGGCTGG
AGTGCAGTGGTG
TGATCGCGGCTCACTGCAGCCTTCACTTCCCGGGTTCAAGCCATTCTCCTGCCTCAGC
CTCCCAAGTAGC
TGGGATTACAGGCACGCATCACTACTTCTGGCTAATTTTTGTATTTTTAGTAGGGAC
AGGGTTTTTCACC
ATGTTGGCCAGGTTGGTCTCAAACTCCTGGCCTCATATGATCTGCCCACCTCGGCCT
CCCAAAGTGCTGG
GATTACAGGCGTGAGCCACTGCGCCTTTCTTTGTATTTGTTCAAGTAATATACTGAA
ATATGTACTGTGC
CTCCCACTTTATGGAGGAGGAAACTGAGGCCAGCAAATGAGGCTGTCATGGGAGGT
GGAGACAGGATTTG
AACCTGCCTCAGTGCAGGAGGCTCAAGAGCCTCTGTCTTCTCTCAGGGCACTGTGTG
GGAGGGTGAGAAG
GAGGGAGGCCCACAGAGGCATGACCTCTGATTGCCACTGTCACCTGGGCCCTGCTCT
CTGAAGTCTCTGC
CAAGCGGGGAGGTGGCCGGGGGAGGGCCCTGCTCTGTGCAGCCTCCCCTCCCCCGG
CCCGCAGAGTTGAG
CACAGAGGGACAGAGGCACGGAACCCCCAGAAATGTCCCTCCTCAGAAACAGGCTC
CAGGCCCTGCCTGC
CCTGTGCCTCTGCGTGCTGGTCCTGGCCTGCATTGGGGGTGAGAAGAAGTGGGTGGA
GGGATGTGGGGCC
CACACCTGGTGGGTGTGAGTGTGGCTGTGTGTCCTGTGGCTCTGTAGCCACGTGAGA
CATGAGTACGGAG
TGTGTGCGTTTCATGGCGTGCGTATGCATGTGCGTGTCGGGGAGTGTGTGTGTCGGT
GGCTGAGAGTGAA
GTGTGAATGTCACATTGGTACAAACTGGGATCATCTGTGTGTGTGCACGTGCGTGCG
TGGAAGTGGGAGT
ATGCAGTCGTGGTAAAAAAGTGCATGTCTGTGTGCATATGTGTATTTGTGTGCACCT
GTCTCTCTGTGGG
GTATGTGTGTGCAAAATATTTGAGTGTGTGGACATGTGTGAGGGGGTGAGTGTGTGC
TGGTGTGTACGTC
TGTGTTTTGCATATGCATTTTTTTTTTTTTTTTTGAGACGGAGTCTCACTCTGTCACCC
AGGCTGGAGTG
CAGTGGTAGCAGTGGTGCGATCTTGGCTCACTGCATCATCCGCCTACCCGTTTCAAG
GGATTCTCCTGCC
TCAGTCTTCAGAGTATTTGGGACTACAGACACACGCCACCATGCCTGGCTAATTTTT
TTTTTTTGAGACG
GAGTCTCGCTCTGTTACCCAGGCTGGAGTGCAGTGGCGTGATCTTGGCTCACTGCAA
CGTCCGCCTCCCG
GGTTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGAGCCCAC
CACCACGCCTGGC
TAATTTTTTGTATTTTTACTAGAGACGGGGTTTCGCCGTGTTAGCCAGGATGGTCTCC
ATATCCTGACCT
CGTGATCCGCCTGCCTCGGCCTTCCAAAGTGCTAGGATTATAGGCGTGAGCCACTGC
GCCTGGCCAATGC
CTGGCTAATTTTTTTATATTTTTGGTAGAGACAGGGTTTTGCCATGTTGCCCAGGCTG
GTCTTGAAATCC
TGACCTCAGGTGATCCGCCCGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGCATGA
GCCACCACGCCCG
GCCATGTACTTTATGTTAAAATGGGATCATATTCTAGATCAGCATTATCCAGTAGAA
ATTTAAATTTTTA
ATACAGGGCCAGGCACGGTGGCTCATGCCTGTAATCCCAGCACTTTCGGAGGCCGA
GGCGGGTGGATCGC
AAGGTCAGGAGATTTGAGATCATCCTGGCTAACAGATGGGTAAAAACCCATCTCTA
CTAAAAATACAAAA
AATTAGCCATGCATGGTGGCATGCGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGG
CCGGAGAATCACTT
GAACCCGGGAGGCAGAGGTTGCAGTGAGCCGAGATCGCGCCACTGCATTCCAACCT
GGGTGACAGAGCGA
GACTCCGTCTGAAAAAAAAAAAAAATTTAACACGTATGTAGACAATGTGCAAGGCA
CCATTCCATGTGCA
TCGTATGTAGTAACTCTTAATTCTCACGATAACCCTGAGGTAGATATTATTACCCCGT
TCTACAAAAGGA
GAAACAGTCCTGGGGAGACAGGATAAGTCACCGGCCAAGGCACACAGCTAGCTACA
TGTGGCCCCCGCGT
GACGGCTGGTCTCTGTAGGCGAGGCTTTGTCCAGATGCGTGGGTAGAAGGTCTGGCC
CGGAAAGAGGAAC
TGACAGCAAGGCTAAGCCAATGTCTGCCCCTGGGGGCAGAAAGTCACCTCCTGCTCT
CCCTCCACTGTCC
ACAGAGGTAGCTCAGACAGGGTGGGGGTCACAGGAGAACGAAGGGAGAAGGGGGT
AGTTCCTGGGCAGCA
AAATCAGGTGGTGAAGGGAGGCATCAGAGGATGGCAATTAGAGAGGCCATTAGAG
GGGAACCACAGGCAG
ACAGGGTGACAGGAGGGACTACTGACACAAGGTGAAGAGATGGCCCAGCCGGACG
GGGTGGCTCACATCT
GTAATCCCAGCATTTTGGGAGCCCGAGGTGGGTGGATCACTTGAGGTCAGGAGTTC
GAGGCCCCAACATG
GCAAAACCCCATCTCTTCTAAAAATACAAAAATTAGCCGGGCATGATGGCAGATGC
CTGTAATCCCTGCT
ACTCGGGAGGCTGAGGCAGGAAAATTGCCTGAATCCAGGAGGTGGAGGTTGCAATG
AGACGAGATCATGA
CACTGCACTCCACCCTGGGCAACAGAGCAAGAGACTGACTCTGTCTCATAAAAAAA
AAGAAAAAAGAAAA
AAAAAAAGAGATGGCTGATGGTTAAAGAGGGGTTAGCGGTCAGGGGACACATAAG
GGTAAAGGCAGGAGG
CAAGAGGACTGGCAGGGGGCTGCCCCTGGGCCACCGGGAGCGACACAGGATGAGC
ATGGAGGGAAAGGGA
GAAGGGGATTCTAGGGTCCCAGCCTACCCAAGTTGCCCTCTGGTTCCACCTAGCATG
CCAGCCAGAGGCC
CAGGAAGGAACCCTGAGCCCCCCACCAAAGCTAAAGATGAGTCGCTGGAGCCTGGT
GAGGGGCAGGATGA
AGGAGCTGCTGGAGACAGTGGTGAACAGGACCAGAGACGGGTGGCAATGGTTCTGG
TGAGGGTGTGCTGG
GCTGGGTGGTGGGAGGGGACTCCTGGGTCTGAGGGAGGAGGGGCTGGGGCCTGGAC
CCCTGAGTCTCAGG
GAGGAGGAAAGGGTGGGAGTGGGGCTGTGACCCCTAGGTCTGGGAGGAGTGGAGG
GTTAGAGCTGAGAGC
AGGAACTCCTAGGTCACAGAGAGGAGCGGATAAATGGGGCAGAGAACACCTGGGG
AGAGCTGGGGCCTCC
ACTGTGATGTCCTCTCTCCTGTAGGAGCCCGAGCACCTTCCGGGGCTTCATGCAGAC
CTACTATGACGAC
CACCTGAGGGACCTGGGTCCGCTCACCAAGGCCTGGTTCCTCGAATCCAAAGACAG
CCTCTTGAAGAAGA
CCCACAGCCTGTGCCCCAGGCTTGTCTGTGGGGACAAGGACCAGGGTTAAAATGTTC
ATAAAAGCCAGGT
GTGGTTGTGGCGGGTGCCTGTAGTCCCAGCTACTCAGGAGGCTGAGGTAGGATGAT
GGCTTGAGCCCAGG
AGTTCGAGACCAGCCTGGGCAACACAGCGAGATCTCTTGGGGGTAAAACAAAAAGA
AAAAAAAAAGTTCA
TACTTCTCCAATAAATAAAGTCTCACCTGTGTCCCTGTCTGGATCCTTCCCCAGTGTG
GCCAGAAAAAAA
CCCACCCCACTGCCTCCCAGGAATCAATGAGTAGAAGAGGTGACACCTGATGGGGA
AGGAAGAGTAGGGA
GGTCGGGAAGGGTATCAAGGAATAACACCCTATTGTGGGCTTGCGGAGAATGGGGG
ACTTCAAGGCGTGT
CAGTTTCAGGAGGGTGAGGGCAGGAGCGTGGGTGGAGTCAGCAGGTCCCCATGATG
GCCCTCACTGAGAG
CTTCGCCCTTGTCTCCTACAAGCTCTGACTCCATTCCCAGTGGGCACCCAGCACCTCC
AACCCCTCCACA
GCCCCCAACCCAGCCTCTGTCGGAGGCGAATTCTCAGAGTGAGGGTTCCCTGTCACT
TGAGAGAAGGTTC
CCTGTGACGTGACCTTGGGGGACGTCATTGCCCTTTCTGTCCCCACCCACCCCCTCCG
CAGTTCTGTTGG
CCAGGACTTTGGCCTAGACAAAGGATGGGGGTTGTGGCTGTGGAGCGGAAGTGGGT
CTCAACCACTATAA
ATCCTCTCTGTGCCCGTCCGGAGCTGGTGAGGACAGCCTGCCAGAGTCTGGTAAGAA
AGGGACTCAGGGT
GCGGGGACAGGGGGGCGTCAGCAGGGAGAGGGCAAAGATCGATAAAGCAGGAATT
TTAAGAGGCACAATA
TTAGAAGCCCGTGTTGGAACCATGACTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
GTGTGTGTGTGTG
AGAGAGAGAGAGAGGGAGATGGAGTCTCGCTATGTAGCCCAGGCTAGACTCAAACT
CCTGGGCTCAAGCA
ATCCTCCTGCCTCAGCCTCCCCAGTAGCTGGGACTACAGGTGCACCACCACACTCCA
CAAATCACAGAAT
TTAGAACTGTAGACTATTTGAGCTTCTGCTTAGAGTTAGGGTGGCTGAGGTGGGGAG
GATCCCTTGAGCC
CAGGAGTTTGAGGATGCAGTGAGCTGTGATCTTGCCACCGTGTTCCAGCCTGGGTGA
CAGAGAAACCCCA
TTTCTAAAAAAGAGAAAGAAAAAGGGATAGGTACAATGGCTCATGCCTGTAATCCC
AGCACTCTGGGAGG
CCGAGGCGGGTGGATCACTTGAGGTCAGGAGTTCGACACCAGCCTTACCAGCATGG
TGAAACCGCATCTA
TACTAAAAATACAAAAATTGGCCGGGTGTGGTAGCATATGCCTGTAATCCCAGCTAT
TCCAGAGGCTGAG
ACAGGAGAATTGCTTGAACCCAGGAAGCGGAGGTTGCAGTGAGCCCAGATCGTGCC
ACTGTACTCTAGCC
TGGGTGACAGAGCAAGACTCAGTCTTGGCGGAAAAAAAGAATGAAAAAATTTAAA
AAACTAAAAAAGAAC
TGTAGGCTGGGCGTGGTGGCTTACACTTGTAATCCAAACGCTTTGGGAGGCCAAGGC
AAACGGATCACTT
GATGTCAGGAGTTGGAGACCAGCCTGGCCAACATGGTGAAACCCCGTCTCTACTAA
AAATACAAAAATTA
GACAGGCATGGTGGTGCATGCTTGTATTTCCAGTTACTCAGGAGGCTGAGGCAGGA
GAATCGCTCGAACC
CGGAAGACAGAGGTTGCGGTGAGCCAAAATTGCGCCATCGCACTCCAGCCTGGGCG
AGAGAACAAGACCT
TGTCTTGGAAAAAAAAAAAGAATTGTAGACCATTTGCTTGTGTTCTTTCTCCGGGGA
TCAGATCTCACCC
TCTTTCTGCCGTACTTCCTCATCTCCTACGTGTGGATGATGATATTGTGCCCTGTGCA
TGTTCTTCGTCA
CCAAAAGTGCCTCTCTCATAGAGCAGGTGAGAACTCAGTGAGGAGATGCAGGGACA
TGAGGTCTGACTTA
GGGCAGAGCCCTAAGGTAACACATTTGATCTACTGTAGGTCCTTAATGGTGTCTGCA
GAGCACCTCCCTG
CACTGACTCAGCCTTAGCAAAGGGCAGAGGCTTTGCTGTGTTCCCTGCTGGGCCCAG
AACTGTTTAGGTG
CTCAAGAAAGCCTTCTAGGCTGGGCTCAGTGGCTCACACCTGTACTCCCAGCACCCT
GGGGAGGCCGAGA
TGGGAGGATCGCTTGAGCCCAGGAGTTCCAGACCAGCCTGGGCAACAAAACAAGTC
TCCCATCTCTACAA
AAGAATAAAAATTAGCAGCTGGGCATGGTGGCTCATGCCTGTAATTCCAGCACTTTG
GGAGGCCAAGGCA
GGCAAATCACTTGAGGTTAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACCC
CATCTCTACTAAAA
ATACAAAAATCAGGTGGGGCACAGTGGCTCAAGCCTGTAATCCTAGCACTTTGGGA
GGCCAAGGTGGGCG
GATCACGAGGTCAGAAGTTCGAGACCAGCCTGGCCAGCATGGTGAAACCCCATCTC
TACTAAAAATACAA
AATATTAGCCGGGCATGGTGGCAGGTGCTTGTGATTCCAGCTCCTTGGGAGGCTGAG
GCAGAAGAATTGC
TAGAACCCTGGAGGCAGAGGTTGCAGTGAGCCGAGAACACGCCACTGCACTCCAGC
CTGGGTGACAGAGC
GAGACTCCATCTCAAAAAATACGAAAACAAAAATCAGCCGGGTGGTGGCGGGTGCC
TGTAATCCCAGCTA
CTGGGGAGGCTGAGGCAGGAGAATTGCTTGAACCTGGGAGGTGGGGGTTGCAGTGA
GCCAAGATTGCACC
ACTGCACTCCAGCCTGGGCAACAGAGTGAGATTCCATCTCAAAAAAGAAAAAAATA
ATAATTAAAATGTT
AAAATCAGGAGTAGAATCACAGAATGTTGGAAAGTGAGGCCCAAGAAGGGGGCTG
TGTCCAAGTCCATGC
ATGGGAAACTTGACTGGGACACCGAGCTCACACAGAGCAGGATCTCAGTCCCCCCC
ACCAGAGTGGGGCG
TGACCACAGGAACAGCCGCCTCCAGTCAGCCTGCCACATGACACCCCCTCAATGTTC
CAGGTCTCTGGAC
ACTATGGGCACACGACTCCTCCCAGCTCTGTTTCTTGTCCTCCTGGTATTGGGATTTG
GTGAGTGTGGGC
TTCCGGGGAGGGAAGCCTTGGGGAGGGGAATGAGCTCCAAGCATCTTCCCAGCCCA
GGCCCTTCTTACCT
CTGCCTCTGCCCTCTCCTCTTCTTCCTTCCTCCTTTCCCCCTGCTGCAGCCCCACGGGC
TCTCCTGACAC
ACTCTCCCCCTGCAGAGGTCCAGGGGACCCAACAGCCCCAGCAAGATGAGATGCCT
AGCCCGACCTTCCT
CACCCAGGTGAAGGAATCTCTCTCCAGTTACTGGGAGTCAGCAAAGACAGCCGCCC
AGAACCTGTACGAG
AAGACATACCTGCCCGCTGTAGATGAGAAACTCAGGTAGCACCTGCCCCTGGAGAA
ATGGGGTCTGGCCC
ATACCACCGACTGCATCCAGGACCCAGAAGTTCAGGCCCCAGCCCCTCCTCCCTCAG
ACCCAGGAGTCCA
GGCCTCAGCCCCTCCTCCCTCAGACCCAGGAGTCCAGGCCCCCAGCCCGTCCTCCCT
CAGACCCAGGAGT
CCAGGCCCCCAGCCCCTCCTCCCTCAGACCCAGGAGTCCAGGTCCCCAGACCCTCCT
CCCTCAGACCCAG
GAGTCCAGGCCCCCAGCCCCTCCTCCCTCTAACCATCTGTGCTTTCTCCCCAGGGACT
TGTACAGCAAAA
GCACAGCAGCCATGAGCACTTACACAGGCATTTTTACTGACCAAGTTCTTTCTGTGC
TGAAGGGAGAGGA
GTAACAGCCAGACCCCCCATCAGTGGACAAGGGGAGAGTCCCCTACTCCCCTGATC
CCCCAGGTTCAGAC
TGAGCTCCCCCTTCCCAGTAGCTCTTGCATCCTCCTCCCAACTCTAGCCTGAATTCTT
TTCAATAAAAAA
TACAATTCAAGTTGCTTCTCATGGATGGCACTGCTTTTCTGAGGACTCAAGGGCCAA
GATGGAGGGGCTG
ACTCAGTCCAGCCAACATTTAATGAGCACCTACTTTATGTATGGAGCTCTAACCCAT
GGGTCCATGGGAA
TAAAGCAGTGAATAGTAACAATAAATAATCGTAACAGCAATTAGAGACTAATCTTT
ATTGAAGTCCGGCT
TTCTCTCTTTTATTTTTTTATTTTTTGAGACAGGGTTTCACTCTGTCACCCAGGCTGGA
CAGCAGTGGTG
CAATCTCGGCTCACTGCACCCTCCGCCTCCCAGGTTCCAGCGATTCTGTGTGCCTCA
GCCTCTGGAGTAG
CTGGGATTATGGGCATGTGCCACTGTGCACGGCTAATGTTTGTATTTTTAGTAGGGT
TTTGCCAGTTGGC
TAGGCTGGTCTCAAACTTCTGACCTCAAGTGATCCACCCGCCTCAGCCTCCCAAAGT
ACTGGGGTGACAG
GCATGAGCCACCGCGCCATGCCAAAATCCAGCCTTCTCATTTGTAAAATAACAAATC
ATAGACACAGCTA
GTCCACAGTGGCCTCTGACAGTCTCCAGTTGTTAACGGTGAATGGTGTCCAGGTTTT
TGGCGTCCTGAAC
AAAGAACTGGACAAAACGCACAAACAAAGCAAGGAAGGAATGAAGGGATTTATTG
AAAACGAACATACAC
TAAACAGTGTGGGAGCGGGCCCAAGCATATGGGTTCAAAAGCCCTGTTACAGAATT
TTTGGGAGTTTAAA
TTCCCCCTAGAGGATTCCCCTTCCACTGGTTACTTCTGGTACACCCTATGTAAATGGA
GAGGATGAAGAA
AAGTTACAAAGTCATTTATGGCCTACCGGAGAGGATGTTTCCTGTTATAGCTGAAGT
GAATTGGCCTTAT
GTTCCCTGCCTCCCTATTTTCCTGCCTTACAGTTGCTGGACTGACACATTTTTTATTTT
ATTTATTTTGT
TTTGAGATAAGGTCTTGCTCTGTCGCCCAGGCTGAAGTGCAGTGGTGAGATCTTGGC
TTACTGTAGCCTT
GACCTTTTGGGCTCAAGAGATCCTCCCATCTCAGCCTCCCAAGTAGTTGGGACTAGG
GATGCCCACTGCC
ACACCTGGCTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTCGTCAAGTTGCCTAGG
CTAGTCTCCAAT
TCCTGGGCTCAAGCGATCTGCCCACCTTGCCCTTCCAAAGTGTTGGGATTACGGGTG
TGAACCACCGCAC
CAGGCCTGGGCTGACACTTTAAACGTGCCATTGCAATCAGTGTATAATGGTCCCGTG
GCAGAGGGAGAAT
GGTGCCCAAGTCAGTACTTCTTGGCTGGGACCCAAGAAGACAAACCTCTAGGGCCG
GCGTGGTGGCTCAC
GCCTGTAATCCCAGCACTTTGGTTGGCTGAGGTGGGCAGATCATCTGAGGTCAGGAG
TTCAAGACCAGCC
TGGCCAACATGGTGAAACCTTGTCTCTAATAAAAACACAAAAATTAGCTAGGGGTG
GCCCGCGCCTGTAA
TCCCAGTTACCTGGAGGCTGAGGCAGGAGAATTGCTTGTACCCTGGAGGCGGAGAT
AGTGCCACTGCACT
CCAGCCTGGGCAGCAGAGTGAGACTCCGTCTCTAAAAAAAAAAAAGAAAAACTTAT
TAAAAAAAAATTGC
AAGCAAAAAGCACAGAAGGCAGAAGGTGCAAATAAGCAGAGGCTCCATGGTCTGA
AGGAGGCCAGTGCTG
GGACCATGAATGAAGGGGGCTGGGGGCCAAGATTCCTGTATCCTGAGGGAGTCCAG
GGTGGTACTGCTCC
CCAAGTCCCATGGGTGGGGCTATTGGTCTCTGGGGATCCACTGCTTGTATTTCTCCTA
ATCTGCTTGGCA
TTAACCTCCTGCCAGGCTTTCAACACCCTTGGGGCAGTTTTCCCAGGTCCTGAGGCT
GGGCAGCTGAGTC
CAGTAAAAAACAGGGGCCCTGGAGAGGGACTGTGGGCAATGCCCCCGAGTCCCCGG
GGCCTGGGAAGAAC
AGAGGCTGCCAAGTGCAGGGGGTGGTGGGGAGGGGAAGACCCTGGAGTAACAATC
CCTGCTGTGTCCACT
TGGTTCCCACGGACTCAAGCCAAGCCAAAGCACTGCCCGGACCAGTTCGGCTTCCA
ACCAATCCTGGTGC
TGGCCT

Apolipoprotein C-IV precursor [Homo sapiens]

NCBI Reference Sequence: NP_001637.1
GenPept Identical Proteins Graphics
>NP_001637.1 apolipoprotein C-IV precursor [Homo sapiens]
MSLLRNRLQALPALCLCVLVLACIGACQPEAQEGTLSPPPKLKMSRWSLVRGRMKELLE
TVVNRTRDGWQ
WFWSPSTFRGFMQTYYDDHLRDLGPLTKAWFLESKDSLLKKTHSLCPRLVCGDKDQG

2JQ3: Structure and Dynamics of Human Apolipoprotein C-IIII

Citation: 
Structure and dynamics of human apolipoprotein CIII
Gangabadage CS, Zdunek J, Tessari M, Nilsson S, Olivecrona G, Wijmenga SS
J. Biol. Chem. (2008) 283 p.17416-27
Abstract
Human apolipoprotein CIII (apoCIII) is a surface component of chylomicrons, very low density
lipoproteins, and high density lipoproteins. ApoCIII inhibits lipoprotein lipase as well as binding
of lipoproteins to cell surface heparan sulfate proteoglycans and receptors. High levels of
apoCIII are often correlated with elevated levels of blood lipids (hypertriglyceridemia). Here, we
report the three-dimensional NMR structure and dynamics of human apo-CIII in complex with
SDS micelles, mimicking its natural lipid-bound state. Thanks to residual dipolar coupling data,
the first detailed view is obtained of the structure and dynamics of an intact apolipoprotein in its
lipid-bound state. ApoCIII wraps around the micelle surface as a necklace of six approximately
10-residue amphipathic helices, which are curved and connected via semiflexible hinges. Three
positively charged (Lys) residues line the polar faces of helices 1 and 2. Interestingly, their three-
dimensional conformation is similar to that of the low density lipoprotein receptor binding motifs
of apoE/B and the receptor-associated protein. At the C-terminal side of apoCIII, an array of
negatively charged residues lines the polar faces of helices 4 and 5 and the adjacent flexible loop.
Sequence comparison shows that this asymmetric charge distribution along the solvent-exposed
face of apoCIII as well as other structural features are conserved among mammals. This structure
provides a template for exploration of molecular mechanisms by which human apoCIII inhibits
lipoprotein lipase and receptor binding.
PDB ID: 2JQ3 Download
MMDB ID: 64092 
PDB Deposition Date: 2007/5/28
Updated in MMDB: 2009/07
Experimental
solution nmr 
Method:
Source Organism: Homo sapiens
Similar Structures: VAST
Name of the transcript Transcript length, bp Protein length, aa UniProt ID
APOC4-202 616 127aa P55056
APOC4-201 348 73aa K7EMC3 -

Tabel. Procese biologice în care este implicate APOC4

Accession ID Term Definition of the biological process
GO:0006629 lipid metabolic process The chemical reactions and
pathways involving lipids,
compounds soluble in an
organic solvent but not, or
sparingly, in an aqueous
solvent. Includes fatty acids;
 neutral fats, other fatty-acid
esters, and soaps; long-chain
(fatty) alcohols and waxes;
sphingoids and other long-
chain bases; glycolipids,
phospholipids and
sphingolipids; and carotenes,
polyprenols, sterols, terpenes
and other isoprenoids.
GO:0006869 lipid transport The directed movement of
lipids into, out of or within a
cell, or between cells, by
means of some agent such as a
transporter or pore. Lipids are
compounds soluble in an
organic solvent but not, or
sparingly, in an aqueous
solvent.
GO:0010890 positive regulation of Any process that increases the
sequestering of triglyceride rate, frequency or extent of
sequestering of triglyceride.
Triglyceride sequestration is
the process of binding or
confining any triester of
glycerol such that it is
separated from other
components of a biological
system.
GO:0034379 very-low-density lipoprotein The non-covalent aggregation
particle assembly and arrangement of proteins
and lipids in the liver to form a
very-low-density lipoprotein
particle.
GO:0034447 very-low-density lipoprotein The process in which a very-
particle clearance low-density lipoprotein
 particle is removed from the
blood via receptor-mediated
endocytosis and its constituent
parts degraded.
GO:0070328 Any process involved in the
triglyceride homeostasis
maintenance of an internal
steady state of triglyceride
within an organism or cell.

Table. Funcții moleculare ale APOC4

Accession ID Term Definition of the molecular
process
GO:0005319 lipid transporter activity Enables the directed movement
of lipids into, out of or within a
cell, or between cells.

Phenotypes, diseases and traits associated with this gene

Speciality(ies)/objective(s) / Test method Number
Alzheimer's disease or HDL levels 16
(pleiotropy)
Blood protein levels 2
Cerebrospinal AB1-42 levels in Alzheimer's 1
disease dementia
Cerebrospinal AB1-42 levels in mild 1
cognitive impairment
Cerebrospinal AB1-42 levels in normal 1
cognition
Cerebrospinal fluid AB1-42 levels 1
Cerebrospinal fluid p-tau levels 1

Cerebrospinal fluid p-tau levels in mild 1

cognitive impairment
Test Purpose(s) Number
Antenatal diagnosis 9

Codul SNP: rs1132899 

Alleles: A/G

Ancestral form: A

MAF: 0.11
Phenotype data: Absent

EUR   A: 0.961 (967) A|A: 0.924 (465) Show

G: 0.039 (39) A|G: 0.074 (37)
G|G: 0.002 (1)
  CEU   A: 0.975 (193) A|A: 0.949 (94) Show
G: 0.025 (5) A|G: 0.051 (5)
  FIN   A: 0.919 (182) A|A: 0.848 (84) Show
G: 0.081 (16) A|G: 0.141 (14)
G|G: 0.010 (1)
  GBR   A: 0.978 (178) A|A: 0.956 (87) Show
G: 0.022 (4) A|G: 0.044 (4)
  IBS   A: 0.991 (212) A|A: 0.981 (105) Show
G: 0.009 (2) A|G: 0.019 (2)
  TSI   A: 0.944 (202) A|A: 0.888 (95) Show
G: 0.056 (12) A|G: 0.112 (12)

EUR A G
CEU 97% 3%
FIN 92% 8%
GBR 98% 2%
IBS 99% 1%
TSI 94% 6%

EUR   A: 0.961 (967) A|A: 0.924 (465) Show

G: 0.039 (39) A|G: 0.074 (37)
G|G: 0.002 (1)
  CEU   A: 0.975 (193) A|A: 0.949 (94) Show
G: 0.025 (5) A|G: 0.051 (5)
  FIN   A: 0.919 (182) A|A: 0.848 (84) Show
G: 0.081 (16) A|G: 0.141 (14)
G|G: 0.010 (1)
  GBR   A: 0.978 (178) A|A: 0.956 (87) Show
G: 0.022 (4) A|G: 0.044 (4)
  IBS   A: 0.991 (212) A|A: 0.981 (105) Show
G: 0.009 (2) A|G: 0.019 (2)
  TSI   A: 0.944 (202) A|A: 0.888 (95) Show
G: 0.056 (12) A|G: 0.112 (12)

EUR AA AG GG
CEU 95% 5%
FIN 84% 14% 1%
GBR 96% 4%
IBS 98% 2%
TSI 89% 11%
 Exercițiul 3.
de ce Dstră, în calitate de doctor, ați avea nevoie de cunoștințe în Bioinformatică? Care
resurse și instrumente (din cele utilizate în exerciții sau identificate individual) le considerați
utile / inutile în practica medicală?
Eu,fiind in calitate de doctor,consider ca as avea nevoie de cunostinte in Bioinformatica.Este o
ramura importanta a medicinii contemporane,care ne ofera acces la baza de date medicale in
orice colt al lume nu am fi si cind nu am fi dorit.Bioinformatica este deosebit de importanta
pentru medicii de profil ingust,cum ar fi medicii-geneticieni.Ei folosesc aceasta informatie
pentru a-i oferi pacientului in tratament individual conform necesitatilor.Sau pentru a determina
si a preveni o boala.Datele statistice sunt utilizate pe larg in ziua de azi.Bioinformatica ne ofera
aceste informatii foarte structurat si calitativ.
care va fi rolul Dstră în medicina de viitor în contextul conceptului de Medicină
Personalizată?
Eu fiind doctor intr-un anumit domeniu o sa folosesc bazele de date,precum si cele statistice
pentru a face tratamentul pacientilor cit mai efectiv si rapid.Fiecare pacient trebuie sa aiba un
tratament individual si un rol important pentru elaborarea lui il joaca baza de date globala si
datele statistice.In asa mod vom putea scadea procentul reactiilor adverse la tratament sau
tratament inefficient,pentru a imbunatati starea pacientilor cit mai efectiv posibil.
- cum ați utiliza informația genomică pentru a lucra cu pacienții?
Eu as utiliza informatia genomica pentru a gasi tratamentul eficient si individual pentru fiecare
pacient in parte,ce semnificativ ar creste productivitatea si calitatea lucrului efectuat.
- ce a fost ușor și ce complicat pentru înțelegerea voastră a informațiilor referitor la
profilarea moleculară, medicina genomică, medicina personalizată și bioinformatică?
Cu medicina moleculara am facut cunstinta inca in anul intii,atunci a fost ceva extraordinar de
interesant si neobisnuit.Daca Bioinformatica ar fi fost un curs aparte – as putea mai bine gasi si
folosi informatia de care am nevoie.
- ce ar fi necesar (concret!) pentru a îmbunătăți înțelegerea materialului respectiv?
Eu cconsider ca ar fi bine sa fie create o baza de date pentru Republica in care traim.in care sa fie
introduce sistematizat genele in care cel mai des se produc schimbari patologiile care duc la
maladii.Medicii trebuie sa cunoasca aceasta informative pentru a-I oferi pacientilor diagnosticul
precoce si tratamenul patologiei date.
explicați definiția “un pacient este ...” în opinia Dstră, din punct de vedere profesional,
dar și în calitate de pacient ?
Un pacient este in primul rind-o individualitate,care necesita atentie deosebita si individuala fata
de medic.Fiecare pacient are drepturile si obligatiunile sale,care trebuie respectate.Totodata
pacientul este partea slaba,care necesita ajutorul nostru,necunoscind informatia pe care o are
medicul si pe care el o poate folosi in viata ,bazindu-se pe logica,cunostinte si experienta
acumulata,gindire clinica.