Enzymes Worksheet Ch. 6 Section 2: Name: . Date

Name:…………………………………………………….
Date: ……………………
Enzymes Worksheet Ch. 6 Section 2

Read the following paragraph to answer the questions below
What are enzymes?
Enzymes are biological catalysts – they speed up the chemical reactions that take place inside
all cells, but without being used up in the process. There are many thousands of different types
of enzymes, and each one catalyzes a different reaction. Cells create enzymes based on
instructions carried in the cell’s genes (DNA).
What are enzymes made of?
Enzymes are protein molecules, and so are made up of amino acids. Most enzymes contain
between 100 and 1,000 amino acids. These amino acids are joined together in a long chain,
which is folded to produce a unique 3D structure. Different types of enzymes have different
shapes and functions because the sequence and type of amino acids in their structure is
different.
Why are enzymes so specific?
Enzymes are very specific about which reactions they catalyze. Only molecules with exactly the
right shape will bind to the enzyme and react. These are the reactant, or substrate, molecules.
The part of the enzyme to which the reactant binds (attaches) is called the active site.
This is a very specific shape and the most important part of the enzyme.
1. a) Fill in the gaps in the following sentences using the words in the box below.
different catalysts function the same amino acids catalysts
i) Enzymes are biological ………………… that speed up chemical reactions in
living organisms.
ii) Enzymes are protein molecules, which are made up of long chains of ………...……….
iii) The sequence and type of amino acids are ………………… in each protein, so they
produce enzymes with many different shapes and functions.

iv) The shape of an enzyme is very important to its ………………….
b) Enzymes catalyze chemical reactions involved in important processes in the

human body. Name one of these processes. Pg. 159 …………………………
c) Label the image with the following terms: active site, reactant, enzyme. Pg.160
……………….....
………..….......
+ …….…...
………...........
...
© Boardworks Ltd 2011 1

Name:……………………………………………………. Date: ……………………
2. a) Explain what would happen if a reactant molecule with a different shape to the
enzyme came into contact with the enzyme’s active site. Pg. 160
_________________________________________________________________________
__________________________________________________________________
________________________________________________________________________
b) Name two factors that affect the rate of enzyme-catalyzed reactions. Pg. 160
_________________________________________________________________________
…
c) i) Infer: What do you think would happen to an enzyme’s reaction rate if the
temperature or pH changed significantly beyond the enzyme’s optimum level?
_________________________________________________________________________
ii) Would this have a positive or negative affect on the body?
_________________________________________________________________________
3. A group of students decided to carry out an investigation to find out how enzyme
activity is affected by temperature changes. They put samples of salivary amylase
and starch into two test tubes. Salivary amylase is an enzyme that breaks down
starch into maltose. Its optimum (best) temperature for activity is around 37°C.
a) What do you think happened to the rate of reaction when they increased the
temperature of the first test tube to 37°C?
_________________________________________________________________________
b) What do you think happened to the enzyme activity when the students decreased
the temperature of the second test tube to O°C?
_________________________________________________________________________
Read the following paragraph to answer the questions below

Functions of Digestive System
There are 3 main functions of the digestive system. The digestive system ingests food, breaks it
down so nutrients can be absorbed, and eliminates what cannot be digested. Mechanical
digestion involves chewing food to break it down into smaller pieces. It also includes the action
of muscles in the stomach and small intestine to moves and churn (mix) the food as it travels.
Chemical digestion involves the breakdown of macromolecules in food into smaller molecules or
monomers. The smaller the substance, the easier these molecules can be absorbed into the
body’s cells.
What are examples of digestive enzymes?
Enzymes are protein molecules that speed up biological reactions. For digestion, it would be to
speed up the rate of breaking down biomolecules, such as carbohydrates, proteins, and lipids.
When you chew, amylase, and enzyme found in saliva, begins the process of chemical
digestion by breaking down starches (complex carbohydrates) into simple sugars such as

Name:……………………………………………………. Date: ……………………
glucose. The acidic environment in the stomach is favorable to the action of pepsin, an
enzyme involved in the process of the chemical digestion of proteins. Proteins in the food you
eat will be broken down into amino acids which can then be used by your cells to rebuild into
proteins your body requires such as muscle. In the small intestine, you can find lipase, an
enzyme involved in the process of the chemical digestion of lipids (fats). The completion of
chemical digestion depends on accessory organs such as the pancreas, where most of the
digestive enzymes are produced.
4. a) Fill in the missing words in the following text about enzymes and digestion.
_______________ enzymes are produced by specialized cells in
the pancreas and digestive tract. From there, the enzymes pass
out of the cells, into the _______________ and small intestine
where they come into contact with food molecules. Here, they
catalyze the _______________ of large molecules turning them into
monomers, which are then more easily absorbed by the body.
b) Write down the name of the nutrient next to the enzyme that breaks it down.
Use the words in the box below. *Use device to look up if needed
fats sucrose starch proteins carbohydrates hydrochloric acid
i) Carbohydrase is an enzyme that breaks down _______________
ii) Protease is an enzyme that breaks down _______________
iii) Lipase is an enzyme that breaks down _______________
iv) Amylase is an enzyme that breaks down _______________
c) Infer: Lactase is an enzyme that helps your body break down milk sugars called
lactose. A person who does not produce enough lactase eats a bowl of ice cream.
What do you think will be the effect on the person?
…………………………………………………………………………………………………………
Infer: Lactose is found in milk products. It is converted by the body into a usable form in a series
of chemical reactions. The diagram shows the series of reactions that convert lactose into a
usable form.

Name:……………………………………………………. Date: ……………………
If enzyme 2 is denatured (destroyed), the levels of which substance will increase?

____________________
1. The enzyme would most likely

affect reactions involving
a. molecule A, only
b. molecule C, only
c. molecule C and D
d. molecules B and D
e. molecules A and C
Use the graph above to answer the following questions. * a pH scale has been provided as
reference
2. What is the optimal pH for both enzymes?
a. Pepsin _____________
b. Trypsin_____________
3. Predict the reactivity of trypsin at pH 14. ______________________________________
4. When do neither enzyme work? ___________________________________________
5. Compare the rate of the pepsin-catalyzed reaction at pH 3 with the rate of the trypsin-
catalyzed reaction at pH 3. ________________________________________________
______________________________________________________________________
6. Based on the graph, what can you infer about the pH of a stomach and an intestine?
_____________________________________________________________________J
ustify your answer. ______________________________________________________

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What are enzymes made of and what is their structure?

What are enzymes made of and what is their structure?

Why are enzymes so specific?

Why are enzymes so specific?

Name:…………………………………………………….

Enzymes Worksheet Ch. 6 Section 2

different catalysts function the same amino acids catalysts

i) Enzymes are biological ………………… that speed up chemical reactions in

produce enzymes with many different shapes and functions.

b) Enzymes catalyze chemical reactions involved in important processes in the

© Boardworks Ltd 2011 1

ii) Would this have a positive or negative affect on the body?

Read the following paragraph to answer the questions below

© Boardworks Ltd 2011 2

_______________ enzymes are produced by specialized cells in

out of the cells, into the _______________ and small intestine

catalyze the _______________ of large molecules turning them into

monomers, which are then more easily absorbed by the body.

fats sucrose starch proteins carbohydrates hydrochloric acid

i) Carbohydrase is an enzyme that breaks down _______________

ii) Protease is an enzyme that breaks down _______________

iii) Lipase is an enzyme that breaks down _______________

iv) Amylase is an enzyme that breaks down _______________

© Boardworks Ltd 2011 3

If enzyme 2 is denatured (destroyed), the levels of which substance will increase?

1. The enzyme would most likely

3. Predict the reactivity of trypsin at pH 14. ______________________________________

4. When do neither enzyme work? ___________________________________________

© Boardworks Ltd 2011 4

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