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Tabp Elisa Report: Standard Table Standard (PPB)

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Eka Saputra

This document reports the results of an ELISA test for detecting furazolidone metabolite in shrimp samples. It presents the standard curve generated from standards of known concentrations, showing a linear relationship. Samples of shrimp were then tested in duplicate and their concentrations calculated based on the standard curve. All sample results were below the detection limit of 0.1 ppb and passed the test. The test was performed using an ELISA kit from Taiwan Advance Bio-Pharm and approved by the listed operator and supervisor.

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© All Rights Reserved
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Download as docx, pdf, or txt

Tabp Elisa Report: Standard Table Standard (PPB)

Uploaded by

Eka Saputra
60 views8 pages
This document reports the results of an ELISA test for detecting furazolidone metabolite in shrimp samples. It presents the standard curve generated from standards of known concentrations, showing a linear relationship. Samples of shrimp were then tested in duplicate and their concentrations calculated based on the standard curve. All sample results were below the detection limit of 0.1 ppb and passed the test. The test was performed using an ELISA kit from Taiwan Advance Bio-Pharm and approved by the listed operator and supervisor.

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AOZ

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This document reports the results of an ELISA test for detecting furazolidone metabolite in shrimp samples. It presents the standard curve generated from standards of known concentrations, showing a linear relationship. Samples of shrimp were then tested in duplicate and their concentrations calculated based on the standard curve. All sample results were below the detection limit of 0.1 ppb and passed the test. The test was performed using an ELISA kit from Taiwan Advance Bio-Pharm and approved by the listed operator and supervisor.

Copyright:

© All Rights Reserved
Download as DOCX, PDF, TXT or read online from Scribd
Download as docx, pdf, or txt
60 views8 pages

Tabp Elisa Report: Standard Table Standard (PPB)

Uploaded by

Eka Saputra
This document reports the results of an ELISA test for detecting furazolidone metabolite in shrimp samples. It presents the standard curve generated from standards of known concentrations, showing a linear relationship. Samples of shrimp were then tested in duplicate and their concentrations calculated based on the standard curve. All sample results were below the detection limit of 0.1 ppb and passed the test. The test was performed using an ELISA kit from Taiwan Advance Bio-Pharm and approved by the listed operator and supervisor.

Copyright:

© All Rights Reserved
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Download as docx, pdf, or txt
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TABP ELISA Report Experiment: AOZ(Furazolidone metabolite)_Shrimp

2017/9/8 15:07 Operator: Eka Safutra Approved by: Melani Safitri Lot: A2405002B
Method: Enzyme-Linked Immunosorbent Assay (ELISA) Kit Developer: Taiwan Advence Bio-Pharm.(www.biotest.com.tw)

Standard Linear Regression


Standard Table
ln Calculate Recovery 6,00
Standard(ppb) Repeat 1 Repeat 2 Average CV% (B/B0 %) Diff. ppb %
4,00

ln(B/B0 %)
1 0 3,175 3,175 3,175 0,00 4,61 0,010
2 0,025 2,073 2,073 2,073 0,00 4,18 0,43 0,031 123,9 2,00
3 0,05 1,860 1,860 1,860 0,00 4,07 0,11 0,042 83,3
0,00
4 0,1 1,412 1,412 1,412 0,00 3,79 0,28 0,088 88,4
ppb: 0,01 0,1 1
5 0,2 1,056 1,056 1,056 0,00 3,50 0,29 0,195 97,6
6 0,5 0,717 0,717 0,717 0,00 3,12 0,39 0,562 112,3 y = -0,366Ln(x) + 2,906 Intercept= 2,906
7 Slope= -0,366 Correlation(R)= 0,9902
Sample Analysis ND=shall Not be Detected↓ Input or not↓ Input or not↓
ln Calculate Sensitiv. Cut Point Result Spike Recovery Revise Correction
No. Sample Repeat 1 Repeat 2 Average CV% (B/B0 %) ppb
Dilution ppb
Interpret Date
ppb ppb ppb ppb % Ratio

1 2,805
216, 217, 218, 219, 220 2,805 2,805 0,00 4,48 0,014 4 0,100 0,5 0,054 0,054 Pass
2 2,805
221, 222, 223, 224, 225 2,805 2,805 0,00 4,48 0,014 4 0,100 0,5 0,054 0,054 Pass
3 226, 227, 228, 229 2,805 2,805 2,805 0,00 4,48 0,014 4 0,100 0,5 0,054 0,054 Pass
4 230, 231, 232, 233 2,805 2,805 2,805 0,00 4,48 0,014 4 0,100 0,5 0,054 0,054 Pass

TABP ELISA Report Experiment: AOZ(Furazolidone metabolite)_Shrimp


2017/8/11 0:00 Operator: Eka Safutra Approved by: Melani Safitri Lot: A2405002B
Method: Enzyme-Linked Immunosorbent Assay (ELISA) Kit Developer: Taiwan Advence Bio-Pharm.(www.biotest.com.tw)

Standard Linear Regression


Standard Table
ln Calculate Recovery 6,00
Standard(ppb) Repeat 1 Repeat 2 Average CV% (B/B0 %) Diff. ppb %
4,00
ln(B/B0 %)

1 0 3,175 3,175 3,175 0,00 4,61 0,010


2 0,025 2,073 2,073 2,073 0,00 4,18 0,43 0,031 123,9 2,00
3 0,05 1,860 1,860 1,860 0,00 4,07 0,11 0,042 83,3
0,00
4 0,1 1,412 1,412 1,412 0,00 3,79 0,28 0,088 88,4
ppb: 0,01 0,1 1
5 0,2 1,056 1,056 1,056 0,00 3,50 0,29 0,195 97,6
6 0,5 0,717 0,717 0,717 0,00 3,12 0,39 0,562 112,3 y = -0,366Ln(x) + 2,906 Intercept= 2,906
7 Slope= -0,366 Correlation(R)= 0,9902
Sample Analysis ND=shall Not be Detected↓ Input or not↓ Input or not↓
ln Calculate Sensitiv. Cut Point Result Spike Recovery Revise Correction
No. Sample Repeat 1 Repeat 2 Average CV% ppb
Dilution ppb
Interpret Date
(B/B0 %) ppb ppb ppb ppb % Ratio

1 216 2,839 2,839 2,839 0,00 4,49 0,013 4 0,100 0,5 0,053 0,053 Pass
2 217 2,805 2,805 2,805 0,00 4,48 0,014 4 0,100 0,5 0,054 0,054 Pass
TABP ELISA Report Experiment: AOZ(Furazolidone metabolite)_Shrimp
2017/8/13 0:00 Operator: Eka Safutra Approved by: Melani Safitri Lot: A2405002B
Method: Enzyme-Linked Immunosorbent Assay (ELISA) Kit Developer: Taiwan Advence Bio-Pharm.(www.biotest.com.tw)

Standard Linear Regression


Standard Table
ln Calculate Recovery 6,00
Standard(ppb) Repeat 1 Repeat 2 Average CV% (B/B0 %) Diff. ppb %
4,00

ln(B/B0 %)
1 0 3,175 3,175 3,175 0,00 4,61 0,010
2 0,025 2,073 2,073 2,073 0,00 4,18 0,43 0,031 123,9 2,00
3 0,05 1,860 1,860 1,860 0,00 4,07 0,11 0,042 83,3
0,00
4 0,1 1,412 1,412 1,412 0,00 3,79 0,28 0,088 88,4
ppb: 0,01 0,1 1
5 0,2 1,056 1,056 1,056 0,00 3,50 0,29 0,195 97,6
6 0,5 0,717 0,717 0,717 0,00 3,12 0,39 0,562 112,3 y = -0,366Ln(x) + 2,906 Intercept= 2,906
7 Slope= -0,366 Correlation(R)= 0,9902
Sample Analysis ND=shall Not be Detected↓ Input or not↓ Input or not↓
ln Calculate Sensitiv. Cut Point Result Spike Recovery Revise Correction
No. Sample Repeat 1 Repeat 2 Average CV% (B/B0 %) ppb
Dilution ppb
Interpret Date
ppb ppb ppb ppb % Ratio

1 218 2,805 2,805 2,805 0,00 4,48 0,014 4 0,100 0,5 0,054 0,054 Pass
2 219 2,713 2,713 2,713 0,00 4,45 0,015 4 0,100 0,5 0,059 0,059 Pass

TABP ELISA Report Experiment: AOZ(Furazolidone metabolite)_Shrimp


2017/8/14 0:00 Operator: Eka Safutra Approved by: Melani Safitri Lot: A2405002B
Method: Enzyme-Linked Immunosorbent Assay (ELISA) Kit Developer: Taiwan Advence Bio-Pharm.(www.biotest.com.tw)

Standard Linear Regression


Standard Table
ln Calculate Recovery 6,00
Standard(ppb) Repeat 1 Repeat 2 Average CV% (B/B0 %) Diff. ppb %
4,00
ln(B/B0 %)

1 0 3,175 3,175 3,175 0,00 4,61 0,010


2 0,025 2,073 2,073 2,073 0,00 4,18 0,43 0,031 123,9 2,00
3 0,05 1,860 1,860 1,860 0,00 4,07 0,11 0,042 83,3
0,00
4 0,1 1,412 1,412 1,412 0,00 3,79 0,28 0,088 88,4
ppb: 0,01 0,1 1
5 0,2 1,056 1,056 1,056 0,00 3,50 0,29 0,195 97,6
6 0,5 0,717 0,717 0,717 0,00 3,12 0,39 0,562 112,3 y = -0,366Ln(x) + 2,906 Intercept= 2,906
7 Slope= -0,366 Correlation(R)= 0,9902
Sample Analysis ND=shall Not be Detected↓ Input or not↓ Input or not↓

ln Calculate Sensitiv. Cut Point Result Spike Recovery Revise Correction


No. Sample Repeat 1 Repeat 2 Average CV% Dilution Interpret Date
(B/B0 %) ppb ppb ppb ppb ppb % Ratio ppb

1 220 2,873 2,873 2,873 0,00 4,51 0,013 4 0,100 0,5 0,051 0,051 Pass
TABP ELISA Report Experiment: AOZ(Furazolidone metabolite)_Shrimp
2017/8/15 0:00 Operator: Eka Safutra Approved by: Melani Safitri Lot: A2405002B
Method: Enzyme-Linked Immunosorbent Assay (ELISA) Kit Developer: Taiwan Advence Bio-Pharm.(www.biotest.com.tw)

Standard Linear Regression


Standard Table
ln Calculate Recovery 6,00
Standard(ppb) Repeat 1 Repeat 2 Average CV% (B/B0 %) Diff. ppb %
4,00

ln(B/B0 %)
1 0 3,175 3,175 3,175 0,00 4,61 0,010
2 0,025 2,073 2,073 2,073 0,00 4,18 0,43 0,031 123,9 2,00
3 0,05 1,860 1,860 1,860 0,00 4,07 0,11 0,042 83,3
0,00
4 0,1 1,412 1,412 1,412 0,00 3,79 0,28 0,088 88,4
ppb: 0,01 0,1 1
5 0,2 1,056 1,056 1,056 0,00 3,50 0,29 0,195 97,6
6 0,5 0,717 0,717 0,717 0,00 3,12 0,39 0,562 112,3 y = -0,366Ln(x) + 2,906 Inte rcept= 2,906
7 Slope= -0,366 Correlation(R)= 0,9902
Sample Analysis ND=shall Not be Detect ed↓ Input or not↓ Input or not↓
ln Calculate Sensitiv. Cut Point Result Spike Recovery Revise Correction
No. Sample Repeat 1 Repeat 2 Average CV% ppb
Dilution ppb
Interpret Date
(B/B0 %) ppb ppb ppb ppb % Ratio

1 221 2,853 2,853 2,853 0,00 4,50 0,013 4 0,100 0,500 0,052 0,052 Pass
2 222 2,835 2,835 2,835 0,00 4,49 0,013 4 0,100 0,500 0,053 0,053 Pass

TABP ELISA Report Experiment: AOZ(Furazolidone metabolite)_Shrimp


2017/8/18 0:00 Operator: Eka Safutra Approved by: Melani Safitri Lot: A2405002B
Method: Enzyme-Linked Immunosorbent Assay (ELISA) Kit Developer: Taiwan Advence Bio-Pharm.(www.biotest.com.tw)

Standard Linear Regression


Standard Table
ln Calculate Recovery 6,00
Standard(ppb) Repeat 1 Repeat 2 Average CV% (B/B0 %) Diff. ppb %
4,00
ln(B/B0 %)

1 0 3,175 3,175 3,175 0,00 4,61 0,010


2 0,025 2,073 2,073 2,073 0,00 4,18 0,43 0,031 123,9 2,00
3 0,05 1,860 1,860 1,860 0,00 4,07 0,11 0,042 83,3
0,00
4 0,1 1,412 1,412 1,412 0,00 3,79 0,28 0,088 88,4
ppb: 0,01 0,1 1
5 0,2 1,056 1,056 1,056 0,00 3,50 0,29 0,195 97,6
6 0,5 0,717 0,717 0,717 0,00 3,12 0,39 0,562 112,3 y = -0,366Ln(x) + 2,906 Intercept= 2,906
7 Slope= -0,366 Correlation(R)= 0,9902
Sample Analysis ND=shall Not be Detected↓ Input or not↓ Input or not↓
ln Calculate Sensitiv. Cut Point Result Spike Recovery Revise Correction
No. Sample Repeat 1 Repeat 2 Average CV% ppb
Dilution ppb
Interpret Date
(B/B0 %) ppb ppb ppb ppb % Ratio

1 223 2,799 2,799 2,799 0,00 4,48 0,014 4 0,100 0,500 0,055 0,055 Pass
TABP ELISA Report Experiment: AOZ(Furazolidone metabolite)_Shrimp
2017/8/20 0:00 Operator: Eka Safutra Approved by: Melani Safitri Lot: A2405002B
Method: Enzyme-Linked Immunosorbent Assay (ELISA) Kit Developer: Taiwan Advence Bio-Pharm.(www.biotest.com.tw)

Standard Linear Regression


Standard Table
ln Calculate Recovery 6,00
Standard(ppb) Repeat 1 Repeat 2 Average CV% (B/B0 %) Diff. ppb %
4,00

ln(B/B0 %)
1 0 3,175 3,175 3,175 0,00 4,61 0,010
2 0,025 2,073 2,073 2,073 0,00 4,18 0,43 0,031 123,9 2,00
3 0,05 1,860 1,860 1,860 0,00 4,07 0,11 0,042 83,3
0,00
4 0,1 1,412 1,412 1,412 0,00 3,79 0,28 0,088 88,4
ppb: 0,01 0,1 1
5 0,2 1,056 1,056 1,056 0,00 3,50 0,29 0,195 97,6
6 0,5 0,717 0,717 0,717 0,00 3,12 0,39 0,562 112,3 y = -0,366Ln(x) + 2,906 Intercept= 2,906
7 Slope= -0,366 Correlation(R)= 0,9902
Sample Analysis ND=shall Not be Detected↓ Input or not↓ Input or not↓

ln Calculate Sensitiv. Cut Point Result Spike Recovery Revise Correction


No. Sample Repeat 1 Repeat 2 Average CV% (B/B0 %) Dilution Interpret Date
ppb ppb ppb ppb ppb % Ratio ppb

1 224 2,819 2,819 2,819 0,00 4,49 0,013 4 0,100 0,500 0,054 0,054 Pass

TABP ELISA Report Experiment: AOZ(Furazolidone metabolite)_Shrimp


2017/8/22 0:00 Operator: Eka Safutra Approved by: Melani Safitri Lot: A2405002B
Method: Enzyme-Linked Immunosorbent Assay (ELISA) Kit Developer: Taiwan Advence Bio-Pharm.(www.biotest.com.tw)

Standard Linear Regression


Standard Table
ln Calculate Recovery 6,00
Standard(ppb) Repeat 1 Repeat 2 Average CV% (B/B0 %) Diff. ppb %
4,00
ln(B/B0 %)

1 0 3,175 3,175 3,175 0,00 4,61 0,010


2 0,025 2,073 2,073 2,073 0,00 4,18 0,43 0,031 123,9 2,00
3 0,05 1,860 1,860 1,860 0,00 4,07 0,11 0,042 83,3
0,00
4 0,1 1,412 1,412 1,412 0,00 3,79 0,28 0,088 88,4
ppb: 0,01 0,1 1
5 0,2 1,056 1,056 1,056 0,00 3,50 0,29 0,195 97,6
6 0,5 0,717 0,717 0,717 0,00 3,12 0,39 0,562 112,3 y = -0,366Ln(x) + 2,906 Inte rce pt= 2,906
7 Slope= -0,366 Correlation(R)= 0,9902
Sample Analysis ND=shall Not be Detected↓ Input or not↓ Input or not↓
ln Calculat e Sensitiv. Cut Point Result Spike Recovery Revise Correction
No. Sample Repeat 1 Repeat 2 Average CV% (B/B0 %) ppb
Dilution
ppb ppb ppb ppb % Ratio ppb
Interpret Date

1 225 2,783 2,783 2,783 0,00 4,47 0,014 4 0,100 0,500 0,055 0,055 Pass
TABP ELISA Report Experiment: AOZ(Furazolidone metabolite)_Shrimp
2017/8/23 0:00 Operator: Eka Safutra Approved by: Melani Safitri Lot: A2405002B
Method: Enzyme-Linked Immunosorbent Assay (ELISA) Kit Developer: Taiwan Advence Bio-Pharm.(www.biotest.com.tw)

Standard Linear Regression


Standard Table
ln Calculate Recovery 6,00
Standard(ppb) Repeat 1 Repeat 2 Average CV% (B/B0 %) Diff. ppb %
4,00

ln(B/B0 %)
1 0 3,175 3,175 3,175 0,00 4,61 0,010
2 0,025 2,073 2,073 2,073 0,00 4,18 0,43 0,031 123,9 2,00
3 0,05 1,860 1,860 1,860 0,00 4,07 0,11 0,042 83,3
0,00
4 0,1 1,412 1,412 1,412 0,00 3,79 0,28 0,088 88,4
ppb: 0,01 0,1 1
5 0,2 1,056 1,056 1,056 0,00 3,50 0,29 0,195 97,6
6 0,5 0,717 0,717 0,717 0,00 3,12 0,39 0,562 112,3 y = -0,366Ln(x) + 2,906 Intercept= 2,906
7 Slope= -0,366 Correlation(R)= 0,9902
Sample Analysis ND=shall Not be Detected↓ Input or not↓ Input or not↓

ln Calculate Sensitiv. Cut Point Result Spike Recovery Revise Correction


No. Sample Repeat 1 Repeat 2 Average CV% ppb
Dilution ppb
Interpret Date
(B/B0 %) ppb ppb ppb ppb % Ratio

1 226 2,778 2,778 2,778 0,00 4,47 0,014 4 0,100 0,500 0,056 0,056 Pass

TABP ELISA Report Experiment: AOZ(Furazolidone metabolite)_Shrimp


2017/8/25 0:00 Operator: Eka Safutra Approved by: Melani Safitri Lot: A2405002B
Method: Enzyme-Linked Immunosorbent Assay (ELISA) Kit Developer: Taiwan Advence Bio-Pharm.(www.biotest.com.tw)

Standard Linear Regression


Standard Table
ln Calculate Recovery 6,00
Standard(ppb) Repeat 1 Repeat 2 Average CV% (B/B0 %) Diff. ppb %
4,00
ln(B/B0 %)

1 0 3,175 3,175 3,175 0,00 4,61 0,010


2 0,025 2,073 2,073 2,073 0,00 4,18 0,43 0,031 123,9 2,00
3 0,05 1,860 1,860 1,860 0,00 4,07 0,11 0,042 83,3
0,00
4 0,1 1,412 1,412 1,412 0,00 3,79 0,28 0,088 88,4
ppb: 0,01 0,1 1
5 0,2 1,056 1,056 1,056 0,00 3,50 0,29 0,195 97,6
6 0,5 0,717 0,717 0,717 0,00 3,12 0,39 0,562 112,3 y = -0,366Ln(x) + 2,906 Intercept= 2,906
7 Slope= -0,366 Correlation(R)= 0,9902
Sample Analysis ND=shall Not be Detected↓ Input or not↓ Input or not↓
ln Calculate Sensitiv. Cut Point Result Spike Recovery Revise Correction
No. Sample Repeat 1 Repeat 2 Average CV% ppb
Dilution ppb
Interpret Date
(B/B0 %) ppb ppb ppb ppb % Ratio

1 227 2,829 2,829 2,829 0,00 4,49 0,013 4 0,100 0,500 0,053 0,053 Pass
2 228 2,739 2,739 2,739 0,00 4,46 0,014 4 0,100 0,500 0,058 0,058 Pass
TABP ELISA Report Experiment: AOZ(Furazolidone metabolite)_Shrimp
2017/8/27 0:00 Operator: Eka Safutra Approved by: Melani Safitri Lot: A2405002B
Method: Enzyme-Linked Immunosorbent Assay (ELISA) Kit Developer: Taiwan Advence Bio-Pharm.(www.biotest.com.tw)

Standard Linear Regression


Standard Table
ln Calculate Recovery 6,00
Standard(ppb) Repeat 1 Repeat 2 Average CV% (B/B0 %) Diff. ppb %
4,00

ln(B/B0 %)
1 0 3,175 3,175 3,175 0,00 4,61 0,010
2 0,025 2,073 2,073 2,073 0,00 4,18 0,43 0,031 123,9 2,00
3 0,05 1,860 1,860 1,860 0,00 4,07 0,11 0,042 83,3
0,00
4 0,1 1,412 1,412 1,412 0,00 3,79 0,28 0,088 88,4
ppb: 0,01 0,1 1
5 0,2 1,056 1,056 1,056 0,00 3,50 0,29 0,195 97,6
6 0,5 0,717 0,717 0,717 0,00 3,12 0,39 0,562 112,3 y = -0,366Ln(x) + 2,906 Intercept= 2,906
7 Slope= -0,366 Correlation(R)= 0,9902
Sample Analysis ND=shall Not be Detected↓ Input or not↓ Input or not↓

ln Calculate Sensitiv. Cut Point Result Spike Recovery Revise Correction


No. Sample Repeat 1 Repeat 2 Average CV% (B/B0 %) ppb
Dilution
ppb ppb ppb ppb % Ratio ppb
Interpret Date

1 229 2,765 2,765 2,765 0,00 4,47 0,014 4 0,100 0,500 0,056 0,056 Pass

TABP ELISA Report Experiment: AOZ(Furazolidone metabolite)_Shrimp


2017/8/28 0:00 Operator: Eka Safutra Approved by: Melani Safitri Lot: A2405002B
Method: Enzyme-Linked Immunosorbent Assay (ELISA) Kit Developer: Taiwan Advence Bio-Pharm.(www.biotest.com.tw)

Standard Linear Regression


Standard Table
ln Calculate Recovery 6,00
Standard(ppb) Repeat 1 Repeat 2 Average CV% (B/B0 %) Diff. ppb %
4,00
ln(B/B0 %)

1 0 3,175 3,175 3,175 0,00 4,61 0,010


2 0,025 2,073 2,073 2,073 0,00 4,18 0,43 0,031 123,9 2,00
3 0,05 1,860 1,860 1,860 0,00 4,07 0,11 0,042 83,3
0,00
4 0,1 1,412 1,412 1,412 0,00 3,79 0,28 0,088 88,4
ppb: 0,01 0,1 1
5 0,2 1,056 1,056 1,056 0,00 3,50 0,29 0,195 97,6
6 0,5 0,717 0,717 0,717 0,00 3,12 0,39 0,562 112,3 y = -0,366Ln(x) + 2,906 Intercept= 2,906
7 Slope= -0,366 Correlation(R)= 0,9902
Sample Analysis ND=shall Not be Detected↓ Input or not↓ Input or not↓
ln Calculate Sensitiv. Cut Point Result Spike Recovery Revise Correction
No. Sample Repeat 1 Repeat 2 Average CV% ppb
Dilution ppb
Interpret Date
(B/B0 %) ppb ppb ppb ppb % Ratio

1 230 2,814 2,814 2,814 0,00 4,48 0,013 4 0,100 0,500 0,054 0,054 Pass
TABP ELISA Report Experiment: AOZ(Furazolidone metabolite)_Shrimp
2017/8/29 0:00 Operator: Eka Safutra Approved by: Melani Safitri Lot: A2405002B
Method: Enzyme-Linked Immunosorbent Assay (ELISA) Kit Developer: Taiwan Advence Bio-Pharm.(www.biotest.com.tw)

Standard Linear Regression


Standard Table
ln Calculate Recovery 6,00
Standard(ppb) Repeat 1 Repeat 2 Average CV% (B/B0 %) Diff. ppb %
4,00

ln(B/B0 %)
1 0 3,175 3,175 3,175 0,00 4,61 0,010
2 0,025 2,073 2,073 2,073 0,00 4,18 0,43 0,031 123,9 2,00
3 0,05 1,860 1,860 1,860 0,00 4,07 0,11 0,042 83,3
0,00
4 0,1 1,412 1,412 1,412 0,00 3,79 0,28 0,088 88,4
ppb: 0,01 0,1 1
5 0,2 1,056 1,056 1,056 0,00 3,50 0,29 0,195 97,6
6 0,5 0,717 0,717 0,717 0,00 3,12 0,39 0,562 112,3 y = -0,366Ln(x) + 2,906 Intercept= 2,906
7 Slope= -0,366 Correlation(R)= 0,9902
Sample Analysis ND=shall Not be Detected↓ Input or not↓ Input or not↓

ln Calculate Sensitiv. Cut Point Result Spike Recovery Revise Correction


No. Sample Repeat 1 Repeat 2 Average CV% (B/B0 %) ppb
Dilution ppb
Interpret Date
ppb ppb ppb ppb % Ratio

1 231 2,790 2,790 2,790 0,00 4,48 0,014 4 0,100 0,500 0,055 0,055 Pass

TABP ELISA Report Experiment: AOZ(Furazolidone metabolite)_Shrimp


2017/9/4 0:00 Operator: Eka Safutra Approved by: Melani Safitri Lot: A2405002B
Method: Enzyme-Linked Immunosorbent Assay (ELISA) Kit Developer: Taiwan Advence Bio-Pharm.(www.biotest.com.tw)

Standard Linear Regression


Standard Table
ln Calculate Recovery 6,00
Standard(ppb) Repeat 1 Repeat 2 Average CV% (B/B0 %) Diff. ppb %
4,00
ln(B/B0 %)

1 0 3,175 3,175 3,175 0,00 4,61 0,010


2 0,025 2,073 2,073 2,073 0,00 4,18 0,43 0,031 123,9 2,00
3 0,05 1,860 1,860 1,860 0,00 4,07 0,11 0,042 83,3
0,00
4 0,1 1,412 1,412 1,412 0,00 3,79 0,28 0,088 88,4
ppb: 0,01 0,1 1
5 0,2 1,056 1,056 1,056 0,00 3,50 0,29 0,195 97,6
6 0,5 0,717 0,717 0,717 0,00 3,12 0,39 0,562 112,3 y = -0,366Ln(x) + 2,906 Intercept= 2,906
7 Slope= -0,366 Correlation(R)= 0,9902
Sample Analysis ND=shall Not be Detected↓ Input or not↓ Input or not↓
ln Calculate Sensitiv. Cut Point Result Spike Recovery Revise Correction
No. Sample Repeat 1 Repeat 2 Average CV% ppb
Dilution ppb
Interpret Date
(B/B0 %) ppb ppb ppb ppb % Ratio

1 232 2,789 2,789 2,789 0,00 4,48 0,014 4 0,100 0,500 0,055 0,055 Pass
TABP ELISA Report Experiment: AOZ(Furazolidone metabolite)_Shrimp
2017/9/7 0:00 Operator: Eka Safutra Approved by: Melani Safitri Lot: A2405002B
Method: Enzyme-Linked Immunosorbent Assay (ELISA) Kit Developer: Taiwan Advence Bio-Pharm.(www.biotest.com.tw)

Standard Linear Regression


Standard Table
ln Calculate Recovery 6,00
Standard(ppb) Repeat 1 Repeat 2 Average CV% (B/B0 %) Diff. ppb %
4,00

ln(B/B0 %)
1 0 3,175 3,175 3,175 0,00 4,61 0,010
2 0,025 2,073 2,073 2,073 0,00 4,18 0,43 0,031 123,9 2,00
3 0,05 1,860 1,860 1,860 0,00 4,07 0,11 0,042 83,3
0,00
4 0,1 1,412 1,412 1,412 0,00 3,79 0,28 0,088 88,4
ppb: 0,01 0,1 1
5 0,2 1,056 1,056 1,056 0,00 3,50 0,29 0,195 97,6
6 0,5 0,717 0,717 0,717 0,00 3,12 0,39 0,562 112,3 y = -0,366Ln(x) + 2,906 Intercept= 2,906
7 Slope= -0,366 Correlation(R)= 0,9902
Sample Analysis ND=shall Not be Detected↓ Input or not↓ Input or not↓

ln Calculate Sensitiv. Cut Point Result Spike Recovery Revise Correction


No. Sample Repeat 1 Repeat 2 Average CV% (B/B0 %) Dilution Interpret Date
ppb ppb ppb ppb ppb % Ratio ppb

1 233 2,715 2,715 2,715 0,00 4,45 0,015 4 0,100 0,500 0,059 0,059 Pass

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