Scribd
Upload
38 views1 page

Revision questions-AGRI 2042

The document discusses several topics related to DNA extraction and analysis techniques including the main steps of DNA extraction, techniques like gel electrophoresis, PCR, Southern blotting and DNA sequencing, principles of gene cloning, differences between genomic and cDNA libraries, the importance of genes within the Ti plasmid of Agrobacterium tumefaciens, the principles and characteristics of binary vectors, and animal transformation.

Uploaded by

grace meunier
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOC, PDF, TXT or read online on Scribd
38 views1 page

Revision questions-AGRI 2042

The document discusses several topics related to DNA extraction and analysis techniques including the main steps of DNA extraction, techniques like gel electrophoresis, PCR, Southern blotting and DNA sequencing, principles of gene cloning, differences between genomic and cDNA libraries, the importance of genes within the Ti plasmid of Agrobacterium tumefaciens, the principles and characteristics of binary vectors, and animal transformation.

Uploaded by

grace meunier
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOC, PDF, TXT or read online on Scribd
You are on page 1/ 1

Revision questions AGRI 2042

1.What are the main steps involved in DNA extraction and what is the strategy
involved to achieve each objective?
2. Techniques of DNA Analysis:Gel Electrophoresis, PCR technique, Southern
Blotting, DNA sequencing, RT-PCR :
-Main steps involved in each technique
-Compare and contrast main strategy involved in DNA sequencing/Southern
Blotting:PCR/RT-PCR
-Compare and contrast research questions which can be answered using each
technique
-Application of each technique in Agriculture
3. Principles of Gene Cloning
-Characteristics of a good cloning vector
-Characteristics of each cloning vector being used
-Suitability of each vector
4. DNA libraries: Differences between Genomic library and cDNA library
5. Agrobacterium tumefaciens-Importance of each gene within Ti Plasmid
-Vir Gene-Functions of different genes
6.Binary vectors:-Principles on which it was based
-Characteristics of Binary vectors
-Optimisation of vectors for effective genetic transformation
7.Animal transformation

You might also like