LABORATORY EXPERIMENT

Physical Chemistry II Lab

Experiment no. 5: Rate of Reaction of Blue Colorant NaOCl

Name:______________ ____________Date Started: February 19, 2020

Group No.: Date Ended: February 26, 2020

I. SPECIFIC OBJECTIVE
1. Determine the rate law from the slopes for each solution.
2. Calculate the concentration of the working solution from Beer’s law

IV. TABULATED DATA AND RESULTS

Working solution: Test tube 1

Wavelength Absorbance (A) lnA

340 -0.51 -0.6733
400 0.155 -1.864
500 0.045 -3.101
600 0.707 -0.3467
700 0.045 -3.101

Table 1: Obtaining
ƛmax for the working solution at wavelength range ƛ 340 – 700

Test tube 1:

Wavelength Absorbance (A) lnA

500 0.045 -3.101
520 0.094 -2.364
540 0.126 -2.071
560 0.208 -1.570
580 0.431 -0.841
600 0.707 -0.346
620 1.272 0.240
640 1.208 0.188
660 0.318 -1.145
680 0.094 -2.364

Table 2: Increments of + 20 were applied for the working solution at ƛmax = 600 from table 1
 Absorbance (lnA) vs. Wavelength (ƛ)
0.5
0
450 500 550 600 650 700
-0.5
Absorbance (lnA)

f(x) = 0.01 x − 7.13

-1 R² = 0.27
-1.5
-2
-2.5
-3
-3.5
Wavelength

Linear () Linear ()

Figure 1: Absorbance (lnA) vs. Wavelength (ƛ) graph of Table 2

At maximum wavelength: 620

Test tube Absorbance (A) lnA

2 1.009 0.0086
3 0.736 -0.3065
4 0.295 -1.2207
5 0.871 -0.1381
Table 3: Absorbance of test tube samples at ƛmax = 620
Water-blue dye solution

Test tube Volume H2O Volume NaOCl blue dye solution

(ml) (ml)
1 2 8
2 4 6
3 6 4
4 8 2
5 5 5

Table 4: Volumetric ratio of Water-NaOCl solutions for test tubes 1-5, with total volume is 10 ml
For 1st run Data:

time Absorbance ln(A) 300 0.34 -1.07881

50 1.3 0.262364 310 0.398 -0.9213

60 1.16 0.14842 320 0.383 -0.95972

70 0.971 -0.02943 330 0.367 -1.00239

80 0.94 -0.06188 340 0.353 -1.04129

90 0.903 -0.10203 350 0.338 -1.08471

100 0.871 -0.13811 360 0.326 -1.12086

110 0.833 -0.18272 370 0.314 -1.15836

120 0.802 -0.22065 380 0.303 -1.19402

130 0.775 -0.25489 390 0.293 -1.22758

140 0.747 -0.29169 400 0.281 -1.2694

150 0.719 -0.32989 410 0.269 -1.31304

160 0.691 -0.36962 420 0.258 -1.3548

170 0.663 -0.41098 430 0.249 -1.3903

180 0.637 -0.45099 440 0.24 -1.42712

190 0.612 -0.49102 450 0.232 -1.46102

200 0.59 -0.52763 460 0.215 -1.53712

210 0.567 -0.5674 470 0.215 -1.53712

220 0.546 -0.60514 480 0.207 -1.57504

230 0.525 -0.64436 490 0.199 -1.61445

240 0.503 -0.68717 500 0.192 -1.65026

250 0.484 -0.72567 510 0.185 -1.6874

260 0.466 -0.76357 520 0.188 -1.67131

270 0.448 -0.80296 530 0.172 -1.76026

280 0.43 -0.84397 540 0.166 -1.79577

290 0.3 -1.20397 550 0.16 -1.83258

 Absorbance(lnA) vs. Time (s)
1st run
0.5
0
0 f(x)100
= − 0 x + 0.24 200 300 400 500 600
Absorbance (lnA) R² = 0.99
-0.5
-1
-1.5
-2
-2.5
Time (s)

Absorbance ln(A) Linear (ln(A))

Figure 2: Graph for first run: Absorbance (lnA) vs. Time (s), and point slope form equation

2nd run Data:

time Absorbance ln(A) 350 0.607 -0.49923

50 1.233 0.20945 360 0.593 -0.52256
60 1.192 0.175633 370 0.58 -0.54473
70 1.166 0.153579 380 0.567 -0.5674
80 1.14 0.131028 390 0.555 -0.58879
90 1.116 0.109751 400 0.544 -0.60881
100 1.091 0.087095 410 0.532 -0.63111
110 1.066 0.063913 420 0.52 -0.65393
120 1.044 0.043059 430 0.508 -0.67727
130 1.019 0.018822 440 0.496 -0.70118
140 0.997 -0.003 450 0.484 -0.72567
150 0.972 -0.0284 460 0.471 -0.7529
160 0.95 -0.05129 470 0.46 -0.77653
170 0.928 -0.07472 480 0.449 -0.80073
180 0.906 -0.09872 490 0.496 -0.70118
190 0.885 -0.12217 500 0.428 -0.84863
200 0.867 -0.14272 510 0.518 -0.65778
210 0.845 -0.16842 520 0.408 -0.89649
220 0.825 -0.19237 530 0.399 -0.91879
230 0.807 -0.21443 540 0.389 -0.94418
240 0.788 -0.23826 550 0.379 -0.97022
250 0.768 -0.26397 560 0.37 -0.99425
260 0.749 -0.28902 570 0.36 -1.02165
270 0.732 -0.31197 580 0.353 -1.04129
280 0.713 -0.33827 590 0.337 -1.08767
290 0.695 -0.36384 600 0.337 -1.08767
300 0.679 -0.38713 610 0.329 -1.1117
310 0.663 -0.41098 620 0.322 -1.1332
320 0.647 -0.43541 630 0.315 -1.15518
330 0.633 -0.45728 640 0.308 -1.17766
340 0.62 -0.47804 650 0.301 -1.20065
660 0.295 -1.22078
670 0.288 -1.24479
680 0.281 -1.2694
 Absorbance(lnA) vs. Time (s)
2nd run

0.5
Absorbance (lnA)

0 f(x) = − 0 x + 0.32
0 R²100
= 0.99 200 300 400 500 600 700 800
-0.5
-1
-1.5
tme (s)

Absorbance Linear (Absorbance)

ln(A) Linear (ln(A))

Figure 3: Graph for second run: Absorbance (lnA) vs. Time (s), and point slope form equation

V. DISCUSSION OF RESULTS

The total volume for test tube samples 1-5 is 10 ml, each sample have different
concentrations and volumetric ratios of water-blue dye solution (NaOCl) as classified in Table
4. The working solution is taken to be test tube 1 . Using UV-VIS the highest absorbance (0.707) of
this sample lies at ƛmax = 600. This wavelength is used to compute for the concentration of the working
solution which is 5.123 ×10−6 M . Also at wavelength 600 increments and decrements of +20 were
considered to obtain the absorbance listed in Table 2. The maximum absorbance is 1.272 at wavelength
620. At wavelength 620, the readings for test tubes 2,3,4 and 5 were taken and is listed from Table 3.
To determine the rate law, slopes for the 1st run and 2nd run must be calculated from the graph.
For the 1st run the slope is found to be -0.0038 as seen from figure 2. The 2nd run has a slope of
-0.0023 as seen from figure 3, and both result means the reactions are first order.

VI. CONCLUSION

In this experiment the order of reaction with respect to NaOCl is tested and is determined to be
first order for both the 1st and the 2nd run.

VII. APPENDICES:

VII.I CALCULATIONS

a. Concentration of working solution: Test tube 1

A=Ɛbc
 A = ƛmax Ɛ =1.38 ×105 b=0.01 m

c= A / Ɛb ¿( 0.707)/¿ )(1 cm )¿ 5.123 ×10−6 M

b. Raw datum:
VIII. ABSTRACT OF THE EXPERIMENT

In this experiment the reaction of blue dye with NaOCl was tested to determine the order of
reaction from the graph plotted at different time intervals. Initially at 50s and followed by 20s.
Absorbance was taken by UV-VIS by 2 trials, one is the working solution—test tube 1 and the
other diluted at half NaOCl concentration.