Reddy & Al-Rajab, Cogent Chemistry (2016), 2: 1220055

http://dx.doi.org/10.1080/23312009.2016.1220055

MEDICINAL CHEMISTRY | RESEARCH ARTICLE

Chemical composition, antibacterial and antifungal
activities of Ruta graveolens L. volatile oils
Desam Nagarjuna Reddy1* and Abdul Jabbar Al-Rajab1

Received: 02 June 2016 Abstract: The Ruta graveolens L. (Rutaceae) plant is used medicinally as a homeo-
Accepted: 27 July 2016
pathic remedy in various areas of the world. In this study, volatile oils were extracted
First Published: 04 August 2016
from the areal parts of the plant which was collected from southern India, then
*Corresponding author: Desam
Nagarjuna Reddy, Center for investigated for its chemical constituents and antimicrobial activities. The volatile oils
Environmental Research & Studies, were extracted by hydrodistillation using a Clevenger apparatus and samples were
Jazan University, P.O. Box 114, Jazan,
Saudi Arabia simultaneously analyzed with GC and GC-MS. As a result, a total of 13 chemical con-
E-mail: [email protected]
stituents were characterized. Representing 100% of the total oil with 2-ketones are
Reviewing editor: the major groups. The principal components were identified, undecanone-2 (43.66%),
Youcef Mehellou, University of
Birmingham, UK 2-nonanone (16.09%), 2-acetoxy tetradecanone (14.49%), and nonyl cyclopropan-
ecarboxylate (9.22%), respectively. The extracted volatile oil showed antibacterial
Additional information is available at
the end of the article activity against gram-positive and gram-negative bacteria, resulting in a number of
common human pathogenic bacteria including methicillin-resistant Staphylococcus
aureus and the yeast Candida albicans. The zone of inhibition is from 12.57 ± 0.03 to
27.10 ± 0.02 mm. The minimum inhibitory concentration (MIC) values were within
the antimicrobial activity range and varied between 0.70 ± 0.04 and 1.58 ± 0.05 μg/
mL. The essential oil showed maximum antifungal activity (35.10 ± 0.02 mm) against
Candida albicans. This study indicates that R. graveolens L. essential oils could be used
as a natural medical application in antimicrobial and antifungal treatments.

ABOUT THE AUTHORS PUBLIC INTEREST STATEMENT

Desam Nagarjuna Reddy (assistant professor, The plants of family Rutaceae are big repository
corresponding author), PhD, has research of secondary metabolites. A number of plants
experience in the area for more than five years. from this family are used in traditional system of
His thrust area of research is Natural products. His medicine for cosmetics, ornamental plants, and
work in the field can be evidenced by more than home gardens to repel snakes in south India. Due
15 papers published in National and international to its antibacterial and antifungal properties, Ruta
journals of repute. His current research interest graveolens L. is an important medicinal plant
focuses mainly on the analysis of bioactive in the countries of Indian subcontinent having
natural compounds and their biological activity morphological resemblance. The synthetic drug
determination. possesses toxic effects including carcinogenic
Abdul Jabbar Al-Rajab earned his PhD degree effect. There is intensive search program
in Agronomy from the prestigious University of throughout the world to look for new natural
Lorraine, France in 2007. Then, he worked at antioxidants. In the present study, we are reporting
the research center of Agriculture and Agri- antibacterial and in vitro antifungal potential
Food Canada in London, Ontario, as NSERC with essential oil composition of Ruta graveolens
postdoctoral fellow. He joined Jazan University L. The extracts and essential oil revealed good
(Saudi Arabia) in 2013 as a scientist at Center for antibacterial and antifungal activity. The essential
Environmental Research and Studies. His research oil possesses 2-aliphatic ketones which are major
interest includes understanding the behavior of compounds. Besides its medicinal properties, the
pollutants in the environment and plants, also herb possesses antioxidant potential, thus it may
the natural products as potential anti-agents or be a good source as nutraceutical. It will also be
bio-pesticides. helpful to upgrade the scientific knowledge of
traditional people.

© 2016 The Author(s). This open access article is distributed under a Creative Commons Attribution
(CC-BY) 4.0 license.

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Subjects: Bioscience; Environment & Agriculture; Food Science & Technology; Physical
Sciences

Keywords: Ruta graveolens L; essential oils; GC; GC-MS; antibacterial activity; antifungal
activity; medicinal plant; Chemical Constituents; aliphatic ketones; 2-Undecanone; 2-Nonanone

1. Introduction
The use of medicinal plants in natural remedies has increased dramatically in recent years. As such,
determination of their chemical constituents and potential medicinal applications has become a
priority for pharmacologists and the scientific community. Aromatic and medicinal plants contain
volatile oils with antibacterial, antifungal, and antioxidant activities (Guttridge & Halliwell, 1994;
Halliwel & Gutteridge, 1990, 1998; Hossain, Shah, Sang, & Sakari, 2012; Lee, Shin, Hwang, & Kim,
2003; Mantle, Eddeb, & Pickering, 2000; Maxwell, 1995; Milos, Mastelic, & Jerkovic, 2000; Ruberto &
Baratta, 2000). Many medicinal plants are adopted from the Rutaceae family which contains about
150 genders and over 1,600 species of medicinal importance around the world (Albarici, Vieira,
Fernandes, Silva, & Pirani, 2010; Januário et al., 2009). Essential oils are known for representing wide
variety of secondary metabolites, due to the presence of aromatic compounds presence, which has
attracted the attention of several research groups, regarding the oils’ chemical constituents and bio-
logical importance (Bizzo, Hovell, & Rezende, 2009; Costa et al., 2010; De La Cruz, 2008; Elaissi et al.,
2011). Ruta graveolens L., commonly known as rue or herb of grace (Anonymous, 2003; Lorenzi &
Matos, 2002), has been used in Brazil and other countries in traditional medicine (Al-Qurainy et al.,
2011; Souza, Stamford, Lima, & Trajano, 2007). Essential oils of R. graveolens L. were reported to
have several therapeutic properties which has increased medicinal and pharmacological interest in
this plant (Ahmad, Faisal, Anis, & Aref, 2010; Mejri, Abderrabba, & Mejri, 2010; Ratheesh, Shyni,
Sindhu, & Helen, 2011; Yamashita, Fernandes Neto, Campos, & Guimarães, 2009). Because, synthetic
chemical drugs may cause harmful side effects, many researchers have been examining and looking
for naturally available chemical constituents from plants with antibacterial and antifungal activities.
Historically, essential oils and their derivatives are sources of therapeutic agent, and antibacterial
effects vary among the different plants materials and synthetic drugs (Freiesleben & Jäger, 2014;
Koehn & Carter, 2005; Silver & Bostian, 1990).

The mechanisms of antibacterial effects are different between the plant materials and the syn-
thetic drugs. Essential oils are reported to have significant efficiency in the treatment of resistant
bacterial strains (Gardete & Tomasz, 2014). Moreover, the use of essential oils naturally extends the
half-life of relevant food products, mainly accountable to antibacterial and antioxidant activity.
Essential oils are more commonly known to possess mixtures of complex chemical compounds that
have good smell and that contribute to therapeutic olfactory use, which is synthesized in the plant
organs. Herein, according to the previous literature, and to the best of our knowledge, this paper (or
work) presents a novel study on the R. graveolens L. species and its use in potential applicability in
pharmacology. Therefore, this research reports on the chemical constituents, antibacterial, and an-
tifungal activities of the essential oil extracted from the aerial parts of R. graveolens L.

2. Materials and methods

2.1. Reagents and chemicals

All analytical grade standards, chemicals, solvents, and reagents were supplied by Sigma-Aldrich
(New Delhi, India). High-purity culture media were supplied by Merck (India).

2.2. Sampling and extraction procedure

Aerial parts of the plant materials were sampled during the flowering stage of the plant in November
2015 from Rayachoty, Rayalaseema region, in southern India. The taxonomic identification of plant
materials was identified by plant taxonomist and its herbarium sheet was deposited at the

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postgraduate laboratory of Botany, Sri Krishnadevaraya University, India. According to the method
described by Viuda-Martos et al. (2011), air-dried areal parts of the plant materials were hydrodis-
tilled for 4 h using a Clevenger apparatus. The oil was separated and dehydrated with anhydrous
Na2SO4. Until further analysis, the essential oil was stored at 4°C.

2.3. Qualitative and quantitative analysis

The compositions of volatile oils extracted from aerial parts of R. graveolens L. were analyzed with
GC and GC-MS. The Agilent GC 6890 N (Agilent, New Delhi, India) was equipped with a flame ioniza-
tion detector FID and a capillary column (HP-5MS 5% 30 m × 0.25 mm × 0.25 μm; Agilent, New Delhi,
India). Gradient temperature program was maintained for the column at 5°C min−1 from 50 to 260°C,
with the carrier gas (Helium, purity 99.99%) at a flow rate of 1.0 mL min−1. The injector temperature
was 300°C, split ratio used was 10:1, and 0.5  μL of each sample was injected manually. For gas
chromatography-mass spectrometry analysis, a GC-MS Agilent 6890  N/5975 (Agilent, India) was
used. The mass detector was operated in the mode of electron impact ionization EI, the mass spec-
tra were obtained with 70 eV in a mass range of 50–500 m z−1 and the GC conditions and column
were as described above. The chemical constituents were identified by their retention time and
compared to the standards and mass spectra referred in the library Wiley 5, Mass-Finder, and Adams
GC/MS libraries (Adams, 2007).

2.4. Antibacterial activity

2.4.1. Sources of microbial strains

The antimicrobial activity of Ruta species essential oils was evaluated using laboratory reference
strains (American Type Culture Collection) “ATCC,” American Research Service (ARC) culture collec-
tion or (NRRL) for Candida albicans and gram-negative and positive; National Museum of Natural
History “NMHN” for filamentous fungi, accessed from Laboratory of microbiology, Centre for Cellular
and Molecular Biology, India. Gram-positive bacteria are: Bacillus cereus (ATCC 10876), Enterobacter
cloacae (ATCC 13047), Enterococcus Faecalis (ATCC 49452), Listeria monocytogenes (ATCC15313),
Staphylococcus aureus (ATCC 25923), Micrococcus flavus (ATCC 25923), Micrococcus luteus (ATCC
9341). Gram-negative bacteria are: Acinetobacter Baumannii (ATCC 19606), Escherichia coli (ATCC
25922), Klebsiella pneumonia (ATCC 700603), Pseudomonas aeruginosa (ATCC 27853), Proteus mira-
bilis (ATCC 35659), Salmonella typhimurium (ATCC 13311), Citrobacter freundii (ATCC 8090),
Enterobacter aerogenes (ATCC 13048). Fungal micro-organisms are: Candida albicans (ATCC 26790),
Aspergillus fumigatus (MNHN 566), Aspergillus flavus (MNHN 994294), Cladosporium herbarum
(MNHN 3369), Fusarium oxysporum (MNHN 963917), Alternaria alternata (MNHN 843390). Strain
numbers and micro-organisms are given in Tables 3 and 4. Strains were stored in fridge at 4°C, and
then 24 h prior to each assay, strains were grown on Luria-Bertani agar. The cultures were diluted
with Mueller–Hinton Broth (MHB) for bacteria which might be used for the antibiotic susceptibility
tests.

2.4.2. Antimicrobial activity by the disc diffusion method

The antimicrobial activity of the extracted volatile oil was determined using the disc diffusion meth-
od according to the NCCLS (NCCLS, 2001; Pfaller, Messer, Karlsson, & Bolmstrom, 1998) using 100.0 μL
of tested micro-organisms suspension, which contains 2 × 108 and 1.5 × 106 CFU/mL for bacteria and
C. albicans, respectively, and 2  ×  105  spores/mL of the tested fungal strains. Sabouraud Dextrose
Agar (SDA) was used to inoculate C. albicans, and bacteria were inoculated overnight at 37°C in
Mueller–Hinton agar (MHA). In order to test the antimicrobial and antifungal activities of the ex-
tracted essential oil, 10.0 μL of the extract was added individually to a 6-mm Whatman filter paper
(Whatman, New Delhi, India), then placed onto the Petri dishes inoculated with the tested micro-
organisms. Treated Petri dishes were kept at 4°C for 2 h. The plates containing bacteria were incu-
bated at 37°C for 24 h, while plates containing C. albicans and fungal strains were incubated at 30°C
for 24 and 48 h, respectively.

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2.4.3. Determination of MIC values

The values of minimum inhibition concentration (MIC) were used to calculate the method of broth
microdilution as described in the guidelines of National Committee for Clinical Laboratory Standards
(NCCLS) for tested bacteria and C. albicans (NCCLS, 2001).

The volatile oils were dissolved in 1% DMSO and all the tests on bacteria and yeast were per-
formed with Mueller–Hinton Broth and Sabouraud Dextrose Broth, respectively. Fresh cultures of
tested strain were prepared and adjusted to 5 × 105 CFU and 2.5 × 106 CFU/mL, for bacteria and C.
albicans, respectively. For bacterial and yeast analysis, all test tubes were inoculated at 37°C and at
30°C, respectively, for 48 h. The essential oils for which micro-organisms did not show visible growth
were determined (MIC). The growth of micro-organism was determined by turbidity, whereas the
MIC values of filamentous fungi were evaluated in agar medium via the dilution method (Soliman &
Badeaa, 2002). Each treatment was used in triplicate. Gentamicin and amphotericin B were used as
the reference compounds to compare antimicrobial, antibacterial, and antifungal activity of the oil.

2.5. Statistical analysis

Experiments in this study were made in triplicates, and data were presented in mean values. The
statistical analysis was conducted by variance test ANOVA (SPSS 21).

3. Results and discussion

3.1. Chemical composition of essential oil

Volatile oil of light yellow color was extracted from the aerial parts of R. graveolens L by hydrodistil-
lation with a 1.29% (v/w). Results of the chemical constituents in the essential oil were determined
and analyzed using GC and GC-MS as shown in Table 1. As a result, a total of 13 chemical constitu-
ents were characterized. Representing 100% of the total oil with 2-ketones are the major groups.
The principal components were identified. Two major compounds are aliphatic 2-ones. The decreas-
ing order of aliphatic ketones is as follows: 2-undecanone (43.66%), 2-nonanone (16.09%), 2-tride-
canone (2.59%), 2-decanone (2.58%), and 2-dodecanone (2.23%).

Table 1. Chemical composition of the essential oil of Ruta graveolens L.

RRI Chemical constituent M.F %
1096 2-Nonanone C9H18O 16.09
1194 2-Decanone C10H20O 2.58
1294 2-Undecanone C11H22O 43.66
1303 2-Undecanol C11H24O 2.19
1315 2-Acetoxy tetradecane C16H32O2 14.49
1331 1-Methyl-5,6-divinyl-1-cyclohexene C11H16 3.38
1397 2-Dodecanone C12H24O 2.23
1498 2-Tridecanone C13H26O 2.59
1552 Nonyl cyclopropanecarboxylate C13H24O2 9.22
1598 2,7-Dimethyl-3,6-dimthylene-1,7-octadiene C12H18 0.63
1828 Ethyl piperonyl acetate C12H14O4 0.43
1866 1,5-Isobutyl-1,3-benzodioxole C11H14O2 0.72
2016 Methyl-3-methylene- C18H22O 1.79
1,2,3,3a,4,4a,4b,5,6,10b-decahydrocyclo-
propa[3,4]cyclohepta[1,2a]naphthalen-8-yl
ether
Total 100.0
Notes: RRI: Relative retention index calculated against n-alkanes on the HP-5MS column; M.F Molecular formula of the
chemical compound; % percentages were calculated from flame ionization detector data.

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Other compounds in the essential oil included, 2-acetoxy tetradecane (14.49%), aliphatic ester
nonyl cyclopropanecarboxylate (9.22%), 1-methyl-5, 6-divinyl-1-cyclohexene (3.38%), 2-Undecanol
(2.19%), methyl-3-methylene-1,2,3,3a,4,4a,4b, 5,6, 10b-decahydrocyclopropa [3,4]cyclohepta
[1,2a]naphthalen-8-yl ether (1.79%), and others that were found in minimal concentration. A com-
parative major chemical constituent of the R. graveolens L., essential oil from various studies, includ-
ing our results, is presented in Table 2.

Our results were not in accord with some published studies regarding the presence of C8 to C13 ali-
phatic 2-ketones along with esters, aldehydes, alcohols, and unsaturated hydrocarbons. From the
Table 2, the profile obtained in the present study was similar to the previous results reported as 2-ali-
phatic ketones. But in the present investigation, the percentage of 2-aliphatic ketones and predomi-
nate constituents (2-undecanone and 2-nonanone) were smaller than the previous results reported
by França Orlanda and Nascimento (2015), Meccia, Rojas, and Usubillaga (2008), were identified
89.94% similarity as aliphatic 2-ketones, predominately, 2-undecanone and 2-nonanone. But the
composition of major constituents are: (França Orlanda & Nascimento, 2015) 2-undecanone
(47.21%) and 2-nonanone (39.17%) and (Meccia et al., 2008) 2-undecanone (43.0%) and 2-nona-
none (33.5%).

The essential oil extracted from the same plant in Algeria presented 81.08% of aliphatic ketones
and major compounds 2-undecanone (55.4%) and 2-nonanone (21.62%) (Farah Haddouchi et al.,
2013). Similar results were reported by Tang, Yang, Yang, and Xu, (2011) in the oil extracted from
plants collected from China where the major compounds 2-undecanone (46.15%) and 2-nonanone
(27.01%) and showed mainly aliphatic ketones (80.04%). Another results reported by De Feo, De
Simone, and Senatore (2002) showed mainly the 2-series of aliphatic ketones (83.4%) and the major

Table 2. Comparative study of R. graveolens L. essential oils with previous results

Major compounds Percentage (%) Place Reference
Undecanone-2, 2-Nonanone, 2-Acetoxy 43.66, 16.09 India Present study
tetradecanone, Nonyl cyclopropanecar-
14.49, 9.22
boxylate
2-Nonanone, Chalepensin, 2-Undeca- 8.97, 7.11, 6.81, 6.66, Colombia Stashenko et al. (2000)
none, Chalepin, Bergapten, Psoralen, 5.54, 5.32, 5.14
Hexatriacontane
2-Undecanone, 2-Heptanol acetate, 33.9, 17.5, 11.0, 10.4, North of Iran Soleimani et al. (2009)
1-Dodecanol, Geyrene, 2-Nonanone 8.8,
2-Undecanone, 2-Nonanone, Pregei- 50.93, 16.85, 8.72 Mérida Janne Rojas et al. (2011)
jerene
2-Undecanone, 2-Nonanone, Pregei- 37,80, 28.28, 6,8 Miranda states
jerene
2-Undecanone 2-Nonanone 46.8, 18.8, Italy De Feo et al., 2002
2-Undecanone 2-Nonanone 55.4, 21.62 Algeria Farah Haddouchi et al.
(2013)
2-Undecanone 2-Nonanone, 2-Acetyl- 46.15, 27.01 China Tang et al. (2011)
tridecane
12.73
2-Undecanone 2-Nonanone, 2-Nonylac- 30.73, 18.06 Malaysia Yaacob et al. (1989)
etate, Xanthotoxin
11.03, 7.24
2-Undecanone 2-Nonanone 49.20, 24.70 Egypt Aboutabl et al. (1988)
2-Undecanone 2-Nonanone 43.0, 33.5 Mérida state Meccia et al. (2008)
2-Undecanone 2-Nonanone 50.50, 17.47 Egypt El-Sherbeny et al. (2007)
2-Nonanone, 2-Undecanone followed by 38.66, 27.34, 12.25, 7.71 Tunisia Fredj et al. (2007)
2-Nonanol, 2-Octyl acetate
2-Undecanone 2-Nonanone 47.21, 39.17 Brazil França Orlanda and Nasci-
mento (2015)

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components like 2-undecanone (46.8%) and 2-nonanone (18.8%) in the essential oil from plants
collected from Italy. In addition, the oil extracted from plants collected in Egypt by El-Sherbeny,
Khalil, and Hussein, (2007) presented 2-undecanone (50.50%) and 2-nonanone (17.47%), aliphatic
compounds (74.86%), and mainly 2-ketones. Aboutabl, Elazzouny, and Hammerschmidt (1988) re-
ported that essential oil extracted from plants growing in Egypt contained mainly aliphatic (76.89%)
ketones, but major compounds were 2-undecanone (49.20%) and 2-nonanone (24.70%). Moreover,
the major constituents of essential oil from two samples collected from Merida and Meridia state in
Venezuela by (Rojas et al., 2011) reported 2-undecanone, 2-nonanone, and pregeijerene, but mainly
the aliphatic 2-ketones from Merida and Meridia state were 74.4 and 72.03%, respectively. The es-
sential oil in samples collected from Tunisia by Fredj, Marzouk, Chraief, Boukef, and Marzouk (2007)
reported 69.77% of aliphatic ketones and major compounds 2-undecanone (38.66%) and 2-nona-
none (27.34%).

In the present study, percentage of aliphatic 2-ketone and major constituents (2-undecanone and
2-nonane) was greater than the values reported from Malaysia (Yaacob, Abdullah, & Joulain, 1989),
North of Iran (Soleimani, Aberoomand-Azar, Saber-Tehrani, & Rustaiyan, 2009), and Colombia
(Stashenko, Acosta, & Martı́nez, 2000) which reported mainly 2-aliphatic ketones (53.24%), (47.13%),
and (18.61%), respectively. Our results were in accord with previous reported results which showed
that aliphatic ketones, which present the major components, were as following: 2-undecanone
43.66% and 2-nonanone 16.09% and others are esters followed by 2-acetoxy tetradecane (14.49%)
and nonyl cyclopropanecarboxylate (9.22%). Abundance of aliphatic ketones in the essential oils
was also in accord with results reported in previous studies. However, the percentage of aliphatic
ketones was comparable with values presented in previous studies. It may be due to the climatic
effect, seasonal genotype, growth location, rainfall, geographic conditions, soil salinity, harvest
­period (Corduan & Reinhard, 1972; Pala-Paul et al., 2005), and the light effect on the composition of
the essential oil.

3.2. Antimicrobial activity

The extracted essential oil was employed in vitro under controlled conditions against micro-­
organisms to analyze its antimicrobial activity. For this assessment, the parameters examined were
the zone of inhibition, zone diameter (mm), and MIC values.

The antibacterial activities of the essential oil against bacteria are shown in Table 3. The present
method (Weerakkody, Caffin, Turner, & Dykes, 2010) measured the zone of inhibition present on the
disk that contained the antibacterial agent. The results in Table 3 display the inhibition zones includ-
ing size of the filter paper (6 mm). A significant variation was observed in the antibacterial properties
of the volatile oil against different bacteria. The volatile oil showed maximum, intermediate, m ­ inimal
(or no) antibacterial activity in inhibition zone >20, <15–20, and >15 mm, respectively. Based on the
zone of inhibition diameter, the volatile oil showed maximum antibacterial activity against
E. Faecalis, B. cereus, S. aureus, P. mirabilis, and M. flavus, with respect to inhibition zones of
27.10 ± 0.02, 26.60 ± 0.03, 23.40 ± 0.06, 22.00 ± 0.02, and 21.20 ± 0.02 mm (p ≤ 0.01). Intermediate
activity was observed against M. luteus, A. baumannii, L. monocytogenes, K. pneumonia, E. cloacae, E.
coli, and C. freundii with 19.06  ±  0.05, 18.60  ±  0.02, 18.30  ±  0.04, 18.10  ±  0.04, 18.00  ±  0.05,
17.70 ± 0.02, and 16.02 ± 0.03 mm inhibition zones (p ≤ 0.01), respectively. Minimal or no activity was
observed against P. aeruginosa and E. aerogenes with 15.30  ±  0.06 and 12.57  ±  0  mm inhibition
zones (p ≤ 0.01), respectively.

By observing Table 3, it can be suggested that this essential oil is a potential antibacterial agent,
because the organisms corresponding to each value of MIC were low and ranged from 0.70 ± 0.04 to
1.58  ±  0.05  μg/mL, with the exception of the standard strain E. aerogenes which required
72.0 ± 0.09 μg/mL to become sensitive to the oil.

The essential oil has a maximum value of MIC at 500 μg/mL, intermediate at MIC 600.0–1,500.0 μg/
mL, and minimum above 1,500 μg/mL (Aligiannis, Kalpoutzakis, Mitaku, & Chinou, 2001). While, the

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Table 3. Zones of growth inhibition (mm) and MIC, showing antibacterial activity of R.
graveolens L. essential oil
Microbial Strains Zone inhibition (mm)a ± standard MIC (μg/mL)b
deviation
Essential oil RAb Essential oil
Gram-positive
Bacillus cereus (ATCC 10876) 26.60 ± 0.03 22.20 ± 0.02 1.0 ± 0.06
Enterobacter cloacae (ATCC 13047) 18.00 ± 0.05 20.04 ± 0.03 0.89 ± 0.05
Enterococcus faecalis (ATCC 49452) 27.10 ± 0.02 23.85 ± 0.05 1.0 ± 0.08
Listeria monocytogenes (ATCC15313) 18.30 ± 0.04 23.40 ± 0.02 0.70 ± 0.04
Staphylococcus aureus (ATCC 25923) 23.40 ± 0.06 19.46 ± 0.04 1.0 ± 0.02
Micrococcus flavus (ATCC 25923) 21.20 ± 0.02 17.06 ± 0.06 1.48 ± 0.09
Micrococcus luteus (ATCC 9341) 19.06 ± 0.05 24.02 ± 0.02 1.0 ± 0.03
Gram-negative
Acinetobacter baumannii (ATCC 19606) 18.60 ± 0.05 19.00 ± 0.08 1.22 ± 0.06
Escherichia coli (ATCC 25922) 17.70 ± 0.02 20.40 ± 0.02 0.65 ± 0.04
Klebsiella pneumonia (ATCC 700603) 18.10 ± 0.04 19.48 ± 0.03 1.58 ± 0.05
Pseudomonas aeruginosa (ATCC 27853) 15.30 ± 0.06 18.24 ± 0.06 1.0 ± 0.07
Proteus mirabilis (ATCC 35659) 22.00 ± 0.02 15.23 ± 0.03 0.76 ± 0.04
Salmonella typhimurium (ATCC 13311) 13.03 ± 0.03 17.04 ± 0.05 1.34 ± 0.05
Citrobacter freundii (ATCC 8090) 16.02 ± 0.03 18.80 ± 0.06 1.0 ± 0.03
Enterobacter aerogenes (ATCC 13048) 12.57 ± 0.03 16.88 ± 0.02 72.0 ± 0.09
a
Values represent means ± standard deviations for triplicate experiments (p < 0.05).
b
 A = reference of antibiotics gentamicin for gram-positive bacteria and mikacin for gram-negative bacteria used was
R
30 μg/dick.

essential oil showed a higher value of MIC for E. aerogenes at 72.0 μg/mL, it generally showed strong
activity for all analyzed bacteria. Further, it showed the lowest antibacterial activity against E. aero-
genes which may be accountable to the high resistance level of gram-negative organisms to the
tested essential oil. Our findings were in accordance with previous studies (Angienda, Onyango, &
Hill, 2010; Gilles, Zhao, An, & Agboola, 2010; Kivrak et al., 2009; Oyedeji, Lawal, Shode, & Oyedeji,
2009) which reported that the volatile oils showed higher efficiency inhibiting the growth of gram-
positive organisms rather than gram-negative organisms.

3.3. Antifungal activity

The essential oil was spiked at a rate of 10 μL per filter paper and demonstrated significant antifun-
gal effects for all tested strains (Table 4). Activity against fungi was compared to that of ampho-
tericin B, a synthetic antifungal drug.

The volatile oil showed maximum, intermediate, and no antifungal activity in zones > 30, <20–30,
and  > 15 mm, respectively. Based on the diameter of the inhibition zones, the essential oil proved to
exhibit the greatest antifungal activity against C. albicans with an inhibition zone of 35.10 ± 0.02 mm
(p ≤ 0.01).

In addition, C. herbarum, A. alternaria, A. flavus, and F. oxysporum (26.06  ±  0.05, 25.60  ±  0.02,
22.30 ± 0.04, and 20.02 ± 0.03 mm) showed intermediate inhibition growth (p ≤ 0.01). Against the oil,
while minimal activity was reported against A. fumigates (14.30 ± 0.06 mm, p ≤ 0.01). On the other
hand, the most significant antifungal activity of the R. graveolens L. extracted oil was reported
against C. albicans with inhibition zones of 35.10 ± 0.02 mm. R. graveolens essential oil activity of the

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Table 4. In vitro antifungal activity of essential oils

Essential oil strains Inhibitory diametersa(mm)
R grievances. L. (10 μL/disc) Amphotericin B. (20 μg/disc)
Alternaria alternata (MNHN 22.30 ± 0.04 25.01 ± 0.04
843390)
Aspergillus flavus (MNHN 25.60 ± 0.02 19.04 ± 0.06
994294)
Aspergillus fumigates (MNHN 14.30 ± 0.06 26.02 ± 0.07
566)
Candida albicans (ATCC 26790) 35.10 ± 0.02 24.00 ± 0.04
Cladosporium herbarum (MNHN 26.06 ± 0.05 34.03 ± 0.09
3369)
Fusarium oxyporum (MNHN 20.02 ± 0.06 11.01 ± 0.05
963917)
Disc diameter (mm) included.
a

fungi F. oxysporum, A. alternaria, A. flavus, and C. albicans was similar to that of amphotericin B.
Table 5 shows MIC values of the essential oil and amphotericin B that correspond to their inhibitory
effects. Against C. albicans, R. graveolens essential oil proved the most inhibition (22.00 ± 0.09 μg/
mL) compared to the other strains, yet amphotericin B had showed greater activity than the oil.
Amphotericin B ranged between 1.00 ± 0.01 and 6.00 ± 0.05 μg/mL, and then greatly increased for
F. oxysporum (25.00  ±  0.09  μg/mL). It is significant to note that compared to the other strains,
A. fumigates proved to be the most sensitive strain to the essential oil.

The correlation between the percentage of the chemical constituents, the most naturally abun-
dant compound and the interactions between them and the antibacterial activity was reported in
previous studies (Delaquis, Stanich, Girard, & Mazza, 2002; Dorman & Deans, 2000). Greater presence
of free heteroatoms such as oxygen-containing components has exhibited contribution to maxi-
mum antibacterial activity (Dorman & Deans, 2000; Lambert, Skandamis, Coote, & Nychas, 2001).
According to (Griffin, Wyllie, Markham, & Leach, 1999; Mailhebiau, 1994), oxygenated terpenes also
show greater antibacterial activity compared normal terpene hydrocarbons. The present study has
further extended knowledge on the contribution of the chemical constituents of volatile oils to anti-
agent properties.

Regarding antibacterial properties (Ben-Bnina, Hammami, Daamii-remadi, Ben-Jannet, & Mighri,

2010; Proestos, Boziaris, Nychas, & Komaitis, 2006), many studies have expressed that essential oils
from Ruta species display minimal potency. The present study shows more antibacterial activity of
the oil toward gram-positive than gram-negative bacteria, due to the natural abundance of ketones
and alcohols in the essential oils. According to Belghazi et al. (2002), Mentha pulegium volatile oil
shows highest antifungal activity against Penicillium and Mucor, because of a major component,
ketone.

Table 5. In vitro MIC values (μg/mL) of essential oils against filamentous fungi and yeast
Essential oil strain R. graveolens L. Amphotericin B
Alternaria alternata (MNHN 843390) 9.80 ± 0.05 1.00 ± 0.01
Aspergillus flavus (MNHN 994294) 9.05 ± 0.03 6.00 ± 0.05
Aspergillus fumigates (MNHN 566) 4.50 ± 0.07 3.51 ± 0.02
Candida albicans (ATCC 26790) 22.00 ± 0.09 3.00 ± 0.02
Cladosporium herbarum (MNHN 3369) 8.50 ± 0.03 3.03 ± 0.06
Fusarium oxyporum (MNHN 963917) 9.06 ± 0.04 25.01 ± 0.09

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4. Conclusion
This study showed that R. graveolens L. chemical compounds have significant antibacterial and an-
tifungal activity properties. Our findings suggest that Ruta graveolens L. essential oils could be used
as a natural source of medicinal application for antimicrobial and antifungal activities. However,
further investigations on other species of the family Rutaceae are encouraged to determine their
potential anti-agent activities.

Funding Ben-Bnina, E., Hammami, S., Daamii-remadi, M., Ben-Jannet, H.,

The authors received no direct funding for this research. & Mighri, Z. (2010). Chemical composition and antimicrobial
effects of Tunisian Ruta chalepensis L. essential oils. Journal
Author details de la Société Chimique de Tunisie, 12, 1–9.
Desam Nagarjuna Reddy1 Bizzo, H. R., Hovell, A. M. C., & Rezende, C. M. (2009). Óleos
E-mail: [email protected] essenciais no Brasil: aspectos gerais, desenvolvimento
ORCID ID: http://orcid.org/0000-0002-5939-9285 e perspectivas [Essential oils in Brazil: General aspects,
development and prospects]. Química Nova, 32, 588–594.
Abdul Jabbar Al-Rajab1
http://dx.doi.org/10.1590/S0100-40422009000300005
E-mail: [email protected]
Corduan, G., & Reinhard, E. (1972). Synthesis of volatile oils in
1
Center for Environmental Research & Studies, Jazan
tissue cultures of Ruta graveolens. Phytochemistry, 11,
University, P.O. Box 114, Jazan, Saudi Arabia.
917–922.
http://dx.doi.org/10.1016/S0031-9422(00)88433-2
Citation information
Costa, J. F. O., Juiz, P., Pedro, A. S., David, J. P. D.,
Cite this article as: Chemical composition, antibacterial
David, L., Giulietti, J. M., … Soares, M. B. P. (2010).
and antifungal activities of Ruta graveolens L. volatile oils,
Immunomodulatory and antibacterial activities of
Desam Nagarjuna Reddy & Abdul Jabbar Al-Rajab, Cogent
extracts from Rutaceae species. Brazilian Journal of
Chemistry (2016), 2: 1220055.
Pharmacognosy, 20, 502–505.
De Feo, V., De Simone, F., & Senatore, F. (2002). Potential
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