410

Bulgarian Journal of Agricultural Science, 25 (No 2) 2019, 410–417

Characteristics and antimicrobial activity of dangke whey fermentation

with sugar addition
Fatma Maruddin1*, Ratmawati Malaka1, Muhammad Taufik2
1
Faculty of Animal Science, Hasanuddin University, Jalan Perintis Kemerdekaan Km.10.Tamalanrea, Makassar,
South Sulawesi 90245, Indonesia
2
Agriculture Development Polytechnic of Gowa, South Sulawesi, Indonesia
*
Corresponding author: [email protected]; [email protected]

Abstract

Maruddin, F., Malaka, R., & Taufik, M. (2019). Characteristics and antimicrobial activity of dangke whey fermen-
tation with sugar addition. Bulgarian Journal of Agricultural Science, 25(2), 410–417

This research was purposed to analyze the chemical and biological characteristics and antimicrobial activity of fermented
whey made from dangke whey as affected by cane sugar. Whey was prepared by mixing dangke whey with sugar at three con-
centrations (9, 12 and 15% w/v). Tapioca was added (about 0.7% w/v) to increase the viscosity of the fermented whey. Lacto-
bacillus plantarum FNCC 0047 was added (about 3% v/v). Increasing the sugar concentration in the dangke whey affected the
solid content of the mixture and the lactose, sucrose, starch and protein content at the end of fermentation was also affected.
The use of sugar concentrations above 9% caused a decrease in total plate count of L. plantarum FNCC 0047 and lactic acid
content. Changes in lactic acid at the end caused changes in the pH. The addition of above 9% sugar caused a decrease in
antimicrobial activity against Escherichia coli and Staphylococcus aureus. The use of sugar up to a concentration of 9% in the
production generated the desired characteristics in terms of nutrition and antimicrobial activity.

Keywords: antimicrobial activity; biological characteristics; chemical characteristics; dangke whey; fermentation; sugar
Abbreviations: ATCC – American Type Culture Collection; MRS – De Man, Rogosa and Sharpe; MRSA – De Man,
Rogosa and Sharpe Agar; FNCC – Food and Nutrition Culture Collection; LBS – Lactobacillus Selection Agar;
pH – Power of hydrogene; CFU – Colony Forming Unit; NA – Nutrient Agar; °C – Degree of Celcius; μL – Mi-
croliter; v/v – Volume per volume; w/v – Weight per volume; ml – Mililiter; mmol – Milimolar; % – Percentage;
mm – Milimeter; SE – Standard of error; ± – Approximately; g – Gram

Introduction such as lactose, soluble proteins, fats, water-soluble vita-

mins and mineral salts (Vinderola et al., 2000). Lactose in
Dangke whey is a by-product of dangke production. dangke whey can be used as a raw material for fermented
Dangke is processed without fermentation but involves products. Lactose can be utilized by bacteria to produce
the coagulation of milk protein using papain enzyme both probiotic and non-probiotic products as nutrients for
(Mukhlisah et al., 2017). The production of cheese from growth (Panesar et al., 2007; Bovo et al., 2014). Whey
10 liters of milk results in approximately 6-9 liters of also has bioactive components (immunoglobulin, lyso-
whey, depending on the type of cheese (Almeida et al, zyme, and lactoferrin) which can inhibit the growth of
2008). Dangke whey has not been fully utilized to date. some pathogenic bacteria (Ramos et al., 2012).
Whey contains about 55% of the total nutrients from milk, The most widely used probiotics include lactic acid
Characteristics and antimicrobial activity of dangke whey fermentation with sugar addition 411

bacteria, specifically Lactobacillus and Bifidobacterium form. Dried cultures were activated with 3 consecutive
species (Rocha et al., 2014). Lactobacillus plantarum is transfers in MRS broth (24 h incubation at 37°C) before
commonly used in the manufacture of fermented milk use (Sieladie et al., 2011). The total bacteria of activated
(Vasiee et al., 2014). L. plantarum can inhibit pathogenic culture were determined before further use. The active
bacteria that are harmful to human health (Kaushik et al., culture in MRS broth was stored in a refrigerator (5°C)
2009; Gaudana et al., 2010; Setyawardani et al., 2014). until use. The culture was propagated every 2 weeks. Sub-
The application of L. plantarum in the manufacture of fer- culture in the form of the frozen solution was stored in
mented beverages can increase the value of dangke whey. Eppendorf tubes at -20°C. The sub-culture consisted of a
Fermentation products can be treated with sweeten- starter culture suspended in MRS broth and glycerol (1:1).
ers such as sugar, fructose, glucose, sucralose or glycerol The frozen culture was thawed in tap water for 10 minutes
(Thaweboon et al., 2011). Sweetener commonly used for before use. The thawed culture can be directly inoculated
milk fermentation products is sugar. Sugars can be sourced into media (2% v/v) and incubated at 37°C (Olson & Ary-
from sugar cane and contain sucrose; their presence is ana, 2008).
responsible for consumer acceptance and the character- Production of fermented whey. Dangke whey was
istics of fermented beverages (Widyastuti et al., 2014). mixed with starch at a concentration of 0.7%, and the ini-
The high sugar content may adversely affect the growth tial volume was determined (initial volume before heat-
of lactic acid bacteria. Strains of bacteria commonly used ing). The whey mixture was then heated and sugar was
in the fermentation process may show a different toler- added (9, 12 or 15%) while stirring for 5 minutes at 70°C
ance to sugar (Thaweboon et al., 2011). In the production (dissolved completely). Heated whey was added to dis-
of fermented products from milk, too high concentrations tilled water to obtain the initial volume. Then, the whey
of sugar before inoculation or incubation periods lead to was pasteurized at 80°C for 30 minutes (Alakali et al.,
unfavorable effects on the fermentation process. This oc- 2008, with modifications), cooled and inoculated with 5%
curs because of changes in the osmotic pressure of milk. of bacterial starter, before being incubated at 37°C for 18
Factors that affect the activity of Lactobacillus in the fer- hours (based on optimum growth).
mentation products include strains of probiotic bacteria, Lactic acid bacteria population (pour plate method).
concentrations of inoculant, incubation temperature, du- Samples (1 ml) were added to 9 ml of sterile water. This
ration of fermentation, storage conditions, pH, sugar con- solution was further diluted stepwise yielding a diluted
centration (osmotic pressure), solids content of the milk, solution with a concentration of 10-1 to 10-8 times lower
the interaction of species used, the factors supporting and than the stock solution. Each dilution 10-6 to 10-8 (1 ml)
inhibiting the growth, storage temperature, and nutrient was poured into a Petri dish (Duplo) and mixed with 15
availability (Vasiee et al., 2014). Therefore, sugar addi- mL of deMan, Rogosa, and Sharp agar (MRSA) media.
tion must be determined precisely to obtain the desired The samples were homogenized and allowed to solidify.
effects and avoid any negative ones. This study aimed Each Petri dish was then incubated at 37°C for 24–48
to analyze the antimicrobial and characteristics of a fer- hours (Othman et al., 2012).
mented beverage made from dangke whey with various Maintenance of bacterial culture for antimicrobial
concentrations of sugar added. test. Escherichia coli FNCC 0091 and S. aureus FNCC
0047 were maintained in Tryptone Soy Broth (Oxoid,
Materials and Methods UK) media. All indicator strains were subcultured twice
prior to use in each experiment to obtain active cultures.
Materials used in the study were dangke whey (dan- Additionally, a sub-culture was prepared in Eppendorf
gke by-product), tapioca (rose brand®, Indonesia), sugar tubes and stored at -20°C, grown in Tryptone Soy broth
(gulaku®, Indonesia), distilled water, and buffer at pH of media, inoculated to use 2% (v/v), and incubated at 37°C
4 or 7. Bacterial media were purchased from Oxoid, UK, (Kar & Misra, 1999).
while other analytical chemicals were obtained from Sig- Total antimicrobial activity of fermented whey. The
ma, USA. The bacteria used as a starter were L. plantarum total antimicrobial activity of fermented whey was as-
FNCC 0027, E. coli FNCC 0091, and S. aureus FNCC sessed using well diffusion method (Seydim & Sarikus,
0047, obtained from the Center for the Study of Food and 2006, with modifications) at different concentrations. E.
Nutrition, Gadjah Mada University, Yogyakarta. coli and S. aureus were used as representatives of patho-
Maintenance of starter cultures. The bacterium L. genic bacteria. The cultures were propagated every two
plantarum FNCC 0027 (dried culture) was obtained in dry weeks. The stock culture was diluted to achieve a popula-
412 Fatma Maruddin, Ratmawati Malaka, Muhammad Taufik

tion of 106 CFU/mL in nutrient agar, denoted as ready- tained glucose multiplied by 0.9 (AOAC, 2005).
to-use cultures. Then, 1 mL of each culture was poured Statistical analysis. Data were analyzed according
into the Petri dish, followed by the addition of 25 mL of to the one-way ANOVA procedure using SPSS 16.0. All
Nutrient Agar (NA). After solidification of the medium, 2 samples were analyzed in duplicate, and all experiments
wells were created in the middle of each Petri dish by us- were conducted in five replicates. The data were present-
ing a stainless steel ring with a 9.6 mm diameter. Approxi- ed as means and standard deviations. The significance
mately 200 μL of fermented whey sample with different of their variance was verified using Duncan’s Multiple
concentrations of sugar (0, 9, 12 and 15%) was poured Range Test (Nahartyo, 2016).
into the good hole and kept at 10°C for 30 minutes before
being incubated at 37°C for 24 hours. The inhibition zone, Results
which was a clear area around the well, was measured by
using a caliper three times in three different places and the Physicochemical characteristics of fermentation me-
result was averaged. The test was measured three times in dia and whey. Different concentrations of sugar in the
duplicate replications. manufacture of fermentation media only affected total
Determination of pH and titratable acidity. The pH solid and sucrose levels (p < 0.05). Our experiments re-
of fermented beverages was measured using a pH meter vealed that increasing the sugar concentration in the fer-
(Hanna). Titratable acidity was measured by a titration mentation medium led to changes in the solids. The re-
method. The lactic acid percentage was calculated using sults shows that the incorporation of 9, 12 and 15% of
the following formula (Othman et al., 2012): sugar (sweeteners) besides results in sugar contents of
only 6.40%, 9.48%, and 12.35%, respectively (Fig. 1).
V × 0.01 × 90.08 × 100
Lactic acid (%) = ––––––––––––––––––––, Lactose, sugar, starch, and protein present in the fer-
10g mentation media declined at the end of fermentation.
where: V is volume of titration. Their reduction was dissimilar, as a result of different
Proximate analysis. Proximate analysis was per- initial sugar concentrations (p < 0.05). However, the fat
formed to determine protein and fat in fermented whey contained in each media with the presence of sugar was
and the analysis were conducted according to AOAC pro- not significant (p > 0.05) (Fig. 2).
cedures (AOAC, 2005). L. plantarum FNCC 0047 cell counts and viability.
Total solids. By directly forced air oven drying, the A decline in the amount of L. plantarum in the fermented
analysis was performed to determine total solids. Total whey was a result of an increase in the sugar concentra-
solids were determined by weighing fermented whey, tion. The activity of L. plantarum FNCC 0047 was depen-
drying, and then weighing the fermented whey residue. dent on growth medium. The most desirable conditions
Experimental samples were dried for 4 h at 100±1°C in for L. plantarum growth were media containing 9% sugar
the oven. The total solids content of fermented beverages and media without sugar addition. In media with more
was the weight of dried fermented beverages residue ex- than 12% sugar, a decrease in L. plantarum FNCC 0047
pressed as % of original fermented beverages test portion
weight (AOAC, 2005).
Lactose. The Munson-Walker gravimetric analysis
was performed to determine lactose according to AOAC
procedures (AOAC, 2005).
Sucrose. IDF_ISO_AOAC Method analysis was per-
formed to determine sugar. The percentage of sugar was
calculated using the following formula, with corrected di-
rect invert readings S={100(-a-b)/[142.35-(t/2)]}×(26/w);
where S = sugar in the test sample; a = the corrected direct
polarization; t = temperature of the polarized solution;
and w = weight test portion taken. The analysis was con-
ducted according to AOAC procedures (AOAC, 2005). Fig. 1. Physicochemical characteristics of fermentation
Starch. Starch content was determined using direct media
acid hydrolysis. The method first determined the weight Different letters in the same line indicate significant
of glucose. Starch by weight was the weight of the ob- difference (p < 0.05)
Characteristics and antimicrobial activity of dangke whey fermentation with sugar addition 413

was observed (p < 0.05), as excessive sugar concentra-

tions led to changes in osmotic pressure and low water
activity (aw) (Fig. 3).
Lactic acid levels in whey media with 9% sugar were
significantly higher (p < 0.05) than in the absence of sugar
and with other sugar levels (Fig. 4). Increasing sugar con-

Fig. 4. Lactic acid content of whey fermented

with different concentrations of sugar
Different letters in the same bar indicate significant
Fig. 2. Physicochemical characteristics of fermented difference (p < 0.05)
whey
Different letters in the same line indicate significant Antibacterial activity on whey fermentation against
difference (p < 0.05) gram positive and negative bacteria. Antibacterial activ-
tent until optimum amount led to improve production of ity against E. coli FNCC 0091 (Gram-negative) and S. au-
lactic acid in highest point. Furthermore, increased sugar reus FNCC 0047 (Gram-positive bacteria) were marked
content in excess amount (12 and 15 % sugar) would in- by presence of inhibition zone of bacteria. Inhibition zone
hibit lactic acid production (Fig. 4). against E. coli FNCC 0091 in the absence of sugar was
Changes in the pH value of fermented whey are caused greater than that of S. aureus FNCC 0047. Overall, in-
by changes in the amount of lactic acid during fermentation. creased of sugar concentration caused inhibiton zone was
The pH is negatively correlated with the lactic acid level smaller. But, inhibition zone of E. coli between 9 and 12
in the fermented whey. The pH value of the whey medium % sugar were not different significantly (Fig. 6).
treated with 9% of sugar was lower than that in the absence
of sugar and with other sugar treatments (P < 0.05) (Fig. 5).

Fig. 3. Lactobacillus plantarum (log10 CFU/ml) of whey Fig. 5. The pH value of whey fermented with different
fermented with different concentrations of sugar concentrations of sugar
Different letters in the same bar indicate significant Different letters in the same bar indicate significant
difference (p < 0.05) difference (p < 0.05)
414 Fatma Maruddin, Ratmawati Malaka, Muhammad Taufik

electrons was released by the oxidation of glyceraldehyde

3-phosphate acid via glycolysis. The outcome of this pro-
cess is reduced to lactic acid. Finally, the resulted metabo-
lite was responsible for characteristics of the fermentation
product (Panesar et al., 2007).
The proportion of carbohydrate used by L. plantarum
FNCC 0027 varies during fermentation and depends on
the presence of more simple components. However, phys-
iological characteristics, both fermentation media and
whey, show a decrease in all components of carbohydrate
at the end of fermentation. Activity of lactic acid and the
number of bacteria from the largest to the smallest; a row
occurred in LBS media with added glucose, fructose, su-
crose, lactose, and galactose (Srinivas et al., 1990).
Fig. 6. Inhibition zone (mm) in fermented whey against
L. plantarum FNCC 0047 was used in the production
E. coli FNCC 0091 and S. aureus FNCC 0047 with dif-
of fermented whey. Such bacteria were also utilized in
ferent concentrations of sugar. Data are expressed as
the preparation of fermented milk, vegetables, and meat
mean of inhibition zone diameter (mm) ± SE
products (sausages) (Sawitzki et al., 2009). L. plantarum
Different letters in the same bar denote statistical significance
(p < 0.05) FNCC 0047 was classified as homofermentative. The
enzymatic activity of these bacteria during fermentation
metabolized complex nutritional components into sim-
Discussion pler components. Content of lactic acid in fermented milk
products was affected by the amount and type of bacteria
Incorporation of sweetener beside result in sugar con- and their ability to break down the carbohydrate compo-
tent indicates that the sugar did not completely contain su- nent. Increased levels of lactic acid led to a decrease in the
crose. The content of fat, protein, lactose, and tapioca was pH of the product (Tamime, 2002).
from dangke whey. The composition of the fermentation L. plantanarum need sugar in optimum amount to im-
medium was affected by the content of the raw material prove their activity and growth. Addition of sugar in the
and the addition of other ,ingredients in the media (Ayar fermentation product concentrations above 7% (w/v) prior
& Burucu. 2013). Dangke whey was the main ingredient to fermentation should be considered, promoting an inhib-
of fermentation media in this research. The fermentation itory effect on microorganisms (Tamime, 2002). Exces-
medium was used as a growth medium for L. plantarum sive sucrose levels (more than 10%) prior to inoculation
FNCC 0027. or incubation periods demonstrated unfavorable effects on
Previous studies have reported that mixed components fermentation conditions due to alterations in the osmotic
are determinant factors for physicochemical characteris- pressure of milk and lower water activity. Environmental
tics. Acelora contained total solids (14.8-15%), protein conditions that are similar to previous conditions promote
(0.34-0.52%) and fat (0.12-0.14%) in the mixture of whey the proper adaptive activity of microorganisms. Changes
butter, cheese, and juice (Cruz et al., 2009). in environmental conditions led to the adjustment of mi-
L. plantarum utilized various carbohydrates for fer- croorganisms (Tamime & Robinson, 1999).
mentation, and also utilized essential amino acids and vi- Factors affecting the activity of Lactobacillus in yo-
tamins for growth. L. plantarum FNCC 0027 required a gurt, among other strains of probiotic bacteria, were nu-
variety of carbohydrates and protein as an energy source. trient availability, level of inoculation, incubation tem-
These materials were initially reformed into simpler com- perature, time of fermentation, storage conditions, pH,
ponents (Wheater, 1955). Some Lactobacillus have the concentration of sugar (osmotic pressure), content of milk
ability to break down corn starch at a concentration of solids, and temperature storage (Vasiee et al., 2014).
0.1-0.2 mg/ml (Lee et al., 2001). Microorganisms metabo- Lactic acid in fermented whey was a product of the
lized complex carbohydrates into monosaccharides. The breakdown of nutritional components in the media. Lac-
formed monosaccharides were then involved in glycoly- tic acid bacteria metabolized complex carbohydrates into
sis to form pyruvic acid. In the presence of lactate dehy- simple carbohydrates. The end product of carbohydrate
drogenase, pyruvic acid received electron pairs. A pair of metabolism is lactic acid.
Characteristics and antimicrobial activity of dangke whey fermentation with sugar addition 415

The lactic acid content of this study is higher than that of the acids formed (Rocha et al., 2015).
of the study using L. acidophilus CRL 636, with media An inhibition zone against E. coli FNCC 0091 and
whey protein concentrates (Pescuma et al., 2010). Lac- S. aureus FNCC 0047 also resulted from the presence of
tic acid content at fermentation times of 12 and 24 hours bacteriocin, a type of secondary metabolite. These sec-
were 32 mmol/ml (0.17%) and 40 mmol/ml (0.22%), re- ondary metabolites are compounds that are synthesized by
spectively. microbes but not the basic physiological needs. L. planta-
Increased acidity basically enhanced the concentra- rum FNCC 0027 is a probiotic bacteria and is Gram-pos-
tion of hydrogen ions, leading to a reduction of pH value itive (Daems et al., 2016). Bacteriocins from Gram-posi-
(Panesar et al., 2007). Whey fermentation in this study tive bacteria contain 30 to 60 amino acids, with activities
(18 hours of incubation) resulted in an average pH value varying from a narrow to a broad spectrum. Bacteriocin
of 4.61. This finding was dissimilar to a previous report can inhibit Gram positive and negative bacteria (Cotelo
which using whey protein concentrate (fermented us- et al., 2013). Research on the inhibition of bacteriocins
ing L. acidophilus CRL 636 and incubated for 12 and 24 from L. plantarum Lp9 and L. plantarum C5CC5276 was
hours), where the pH value was 5.5 and 4.8 (Pescuma et reported previously (Kaushik et al., 2009). The antibacte-
al., 2010). The pH of soygurt added lactose (incubated for rial properties not only inhibit E. coli but also retard the
18 hours) using S. thermophilus and L. bulgaricus was growth of B. cereus, Listeria monocytogenes, Salmonella
around 3.96. These discrepant data may result from differ- typhi and S. aureus. L. plantarum ATCC 8014 can inhibit
ences in material or growth media, type and strain of bac- Shigella dysentery, S. aureus ATCC 6538, Pseudomonas
teria, the concentration of inoculum, incubation time, and aeruginosa ATCC 5668, Salmonella typhi and E. coli S5
the availability of nutrients (Yusmarini & Efendi, 2004). (Gaudana et al., 2010).
Factors that influencing the activity of Lactobacillus in Bioactive components contained in the whey, includ-
yogurt included the strain of probiotic bacteria, nutrient ing lysozyme, lactoperoxidase, and lactoferrin among
availability, concentration of inoculation, incubation tem- others, also contribute to the inhibition of some bacte-
perature, fermentation time, storage conditions, pH, con- rial pathogens. Lactoferrin was an iron-binding glyco-
centration of sugar (osmotic pressure), solids content of
protein. Most microorganisms need iron for growth. The
the milk, and storage temperature (Vasiee et al., 2014).
inhibitory activity of bacteria by lactoferrin is through the
Zone of inhibition was observed in previous report,
limitation of iron for growth. A plausible mechanism of
against Gram-positive and negative bacteria, including S.
bacterial inactivation by lysozymes is disruption of the
aureus, L. monocytogenes, Salmonella typhmurium, Shi-
glycosidic bond formation between the two components
gella flexneri, E. coli, Klebsiella pneumoniae, Bacillus
of the peptidoglycan found in the cell walls of bacteria.
cereus, Pseudomonas aeruginosa, and Enterobacter spp.
Bacteria were inhibited by lactoperoxidase through the
Brucella abortus (Mkrtchyan et al., 2010). Additionally,
oxidation of sulfhydryl groups of the cell membrane. Lac-
inhibitory effect of pure isolates L. plantarum L. p9 and
toperoxidase generally shows bacteriostatic properties
L. plantarum C5CC5276 against E. coli, resulting in in-
against Gram positive and Gram negative bacteria in the
hibitory zones of 16 and 20 mm, respectively (Kaushik et
al., 2009). form of bacteriolysis (Murata et al., 2013).
The inhibition zone against E. coli FNCC 009 and
S. aureus FNCC 0047 was the result of the antibacterial Conclusion
activity of fermented whey, which may result from pri-
mary metabolites such as lactic acid. Lactic acid is the The application of various sugar concentrations af-
end-product of carbohydrate metabolism of L. plantarum fected the chemical and biological characteristics and an-
FNCC 0047 in whey beverage products. Lactic acid at- tibacterial activity of fermented whey. Whey fermented
tenuated the pH values and inhibited microbial growth. with sugar concentrations above 9% reduced antibacte-
Primary metabolites are chemical compounds produced rial activity against E. coli and S. aureus and altered their
by microbes, and are needed for growth. One of the pri- chemical and biological characteristics.
mary metabolites was lactic acid. Mostly, pH condition
for the optimum growth of microorganisms ranged from Acknowledgements
6.6 to 7.5 (neutral) (McNeil & Harvey, 2008). Food acidi- Authors would like to thank to Directorate of Higher
fication, at least, may provide double antimicrobial prop- Education for funding this research and Research Insti-
erties: effect on pH and the nature of the typical inhibition tutes and community service of Hasanuddin University
416 Fatma Maruddin, Ratmawati Malaka, Muhammad Taufik

for cooperation and assistance. We also thank to Faculty Limaki, H. K. (2010). Purification, characterisation and
of Animal Husbandry for providing research facilities. identification of acidocin LCHV, an antimicrobial peptide
produced by Lactobacillus acidophilus nv Er 317/402 strain
Narine. International Journal дf Antimicrobial Agents,
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Received: 18.01.2018; Accepted: 04.04.2018