Absorbance Versus Concentration of P-Nitrophenol

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Concentration Absorbance

0 0
0 0.001
0.05 0.05
0.1 0.09
0.2 0.212 Absorbance versus Concentra
0.3 0.399 0.45
0.4
0.35 f(x) = 1.27815028901734 x − 0.01313294797687
R² = 0.977301305954845
0.3

Absorbance
0.25
0.2
0.15
0.1
0.05
0
0 0.05 0.1 0.15
Absorbance versus Concentration of p-nitrophenol

f(x) = 1.27815028901734 x − 0.013132947976879


R² = 0.977301305954845

0 0.05 0.1 0.15 0.2 0.25 0.3 0.35


Concentration of p -nitrophenol (μmol)

Unknown absorbance 0.32


unknown concentration 0.25718
pH enzymatic activity
4 0.00115
6 0.00121
9 0.02024
13 0.00167
Graph of Enzymatic Activity versu
0.025

Enzymatic Activity (μmol min-1)


0.02

0.015

0.01

0.005

0
2 4 6 pH 8 10
nzymatic Activity versus pH

6 pH 8 10 12 14
Temperature Enzymatic Activity

E n z y m a ti c A cti v ity (μ m o l m in -1 m g -1 )
5°C 0.00136
29°C 0.0217
37°C 0.0205
50°C 0.0198
Enzymatic Activity
0.025

0.02

Graph of Enzymatic Activity Versus Te


0.015
0.025

0.02
0.01
0.015

0.005
0.01

0.005
0
0.5 1 1.5 2 2.5 3
0
5°C 29°C 37
Temperature
Enzymatic Activity

ymatic Activity Versus Temperature

1.5 2 2.5 3 3.5 4 4.5


29°C 37°C 50°C
Temperature

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