International Union For The Protection of New Varieties of Plants
International Union For The Protection of New Varieties of Plants
International Union For The Protection of New Varieties of Plants
TG/123/4
ORIGINAL: English
DATE: 2010-03-24
BANANA
GUIDELINES
Alternative Names:*
The purpose of these guidelines (“Test Guidelines”) is to elaborate the principles contained in the General
Introduction (document TG/1/3), and its associated TGP documents, into detailed practical guidance for the
harmonized examination of distinctness, uniformity and stability (DUS) and, in particular, to identify
appropriate characteristics for the examination of DUS and production of harmonized variety descriptions.
ASSOCIATED DOCUMENTS
These Test Guidelines should be read in conjunction with the General Introduction and its
associated TGP documents.
*
These names were correct at the time of the introduction of these Test Guidelines but may be revised or updated.
[Readers are advised to consult the UPOV Code, which can be found on the UPOV Website (www.upov.int), for the
latest information.]
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1.1 These Test Guidelines apply to all varieties of Musa acuminata Colla and
Musa × paradisiaca L. (M. acuminata Colla x M. balbisiana Colla).
1.2 It is noted that cultivated bananas have been derived from wild species Musa acuminata
(A) and Musa balbisiana (B) either alone or in combinations. The cultivated bananas are
classified into botanical groups according to their genome combination. The main groups found
in the edible bananas, natural varieties or hybrids, are AA, AB, AAA, AAB, ABB, AAAA,
AAAB and AABB.
2. Material Required
2.1 The competent authorities decide on the quantity and quality of the plant material required
for testing the variety and when and where it is to be delivered. Applicants submitting material
from a State other than that in which the testing takes place must ensure that all customs
formalities and phytosanitary requirements are complied with.
2.2 The material is to be supplied in the form of corms (whole), rhizomes or in vitro plants.
2.3 The minimum quantity of plant material, to be supplied by the applicant, should be:
2.4 The plant material supplied should be visibly healthy, not lacking in vigor, nor affected by
any important pest or disease.
2.5 The plant material should not have undergone any treatment which would affect the
expression of the characteristics of the variety, unless the competent authorities allow or request
such treatment. If it has been treated, full details of the treatment must be given.
3. Method of Examination
The minimum duration of tests should normally be two independent growing cycles. It is
essential that the plants produce a satisfactory crop of fruit in each of the two growing cycles. In
particular, observations should not be made on the first crop of fruit.
Tests are normally conducted at one place. In the case of tests conducted at more than
one place, guidance is provided in TGP/9 “Examining Distinctness”.
The tests should be carried out under conditions ensuring satisfactory growth for the
expression of the relevant characteristics of the variety and for the conduct of the examination.
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3.4.2 The design of the tests should be such that plants or parts of plants may be removed for
measurement or counting without prejudice to the observations which must be made up to the
end of the growing cycle.
Unless otherwise indicated, all observations should be made on 15 plants or parts taken
from each of 15 plants.
4.1 Distinctness
It is of particular importance for users of these Test Guidelines to consult the General
Introduction prior to making decisions regarding distinctness. However, the following points are
provided for elaboration or emphasis in these Test Guidelines.
The differences observed between varieties may be so clear that more than one growing
cycle is not necessary. In addition, in some circumstances, the influence of the environment is
not such that more than a single growing cycle is required to provide assurance that the
differences observed between varieties are sufficiently consistent. One means of ensuring that a
difference in a characteristic, observed in a growing trial, is sufficiently consistent is to examine
the characteristic in at least two independent growing cycles.
Determining whether a difference between two varieties is clear depends on many factors,
and should consider, in particular, the type of expression of the characteristic being examined,
i.e. whether it is expressed in a qualitative, quantitative, or pseudo-qualitative manner.
Therefore, it is important that users of these Test Guidelines are familiar with the
recommendations contained in the General Introduction prior to making decisions regarding
distinctness.
4.2 Uniformity
4.2.1 It is of particular importance for users of these Test Guidelines to consult the General
Introduction prior to making decisions regarding uniformity. However, the following points are
provided for elaboration or emphasis in these Test Guidelines:
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4.3 Stability
4.3.1 In practice, it is not usual to perform tests of stability that produce results as certain as
those of the testing of distinctness and uniformity. However, experience has demonstrated that,
for many types of variety, when a variety has been shown to be uniform, it can also be
considered to be stable.
4.3.2 Where appropriate, or in cases of doubt, stability may be tested, either by growing a further
generation, or by testing a new plant stock to ensure that it exhibits the same characteristics as
those shown by the previous material supplied.
5.1 The selection of varieties of common knowledge to be grown in the trial with the candidate
varieties and the way in which these varieties are divided into groups to facilitate the assessment
of distinctness are aided by the use of grouping characteristics.
5.2 Grouping characteristics are those in which the documented states of expression, even
where produced at different locations, can be used, either individually or in combination with
other such characteristics: (a) to select varieties of common knowledge that can be excluded
from the growing trial used for examination of distinctness; and (b) to organize the growing trial
so that similar varieties are grouped together.
5.4 Guidance for the use of grouping characteristics, in the process of examining distinctness,
is provided through the General Introduction.
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Standard Test Guidelines characteristics are those which are approved by UPOV for
examination of DUS and from which members of the Union can select those suitable for their
particular circumstances.
Asterisked characteristics (denoted by *) are those included in the Test Guidelines which
are important for the international harmonization of variety descriptions and should always be
examined for DUS and included in the variety description by all members of the Union, except
when the state of expression of a preceding characteristic or regional environmental conditions
render this inappropriate.
States of expression are given for each characteristic to define the characteristic and to
harmonize descriptions. Each state of expression is allocated a corresponding numerical note for
ease of recording of data and for the production and exchange of the description.
Where appropriate, example varieties are provided to clarify the states of expression of
each characteristic.
6.5 Legend
Example Varieties/
Exemples/ Note/
English français deutsch Español
Beispielssorten/ Nota
Variedades ejemplo
QN very short très court sehr kurz muy corta Dwarf Cavendish, 1
Salta do Cacho
very long très long sehr lang muy larga Branca, Gros Michel, 9
Prata, Thap Maeo
Example Varieties/
Exemples/ Note/
English français deutsch Español
Beispielssorten/ Nota
Variedades ejemplo
QN absent or weak absent ou faible fehlend oder gering ausente o débil Grand Nain 1
QN absent or very weak absente ou très faible fehlend oder sehr ausente o muy débil Bluggoe, Figo, 1
gering Figue Pomme Nain
very strong très forte sehr stark muy fuerte Petite Naine 9
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Example Varieties/
Exemples/ Note/
English français deutsch Español
Beispielssorten/ Nota
Variedades ejemplo
10. Plant: compactness Plante : densité de Pflanze: Dichte der Planta: compacidad
of crown la couronne Krone de la corona
(+)
11. Plant: growth habit Plante : port Pflanze: Wuchsform Planta: porte
(*)
(+)
12. Petiole: attitude of Pétiole : port Blattstiel: Haltung Peciolo: porte de las
wings at base des ailes à la base der Flügel an der alas en la base
(+) Basis
QN curved outwards courbé vers auswärts gebogen curvado hacia el French Plantain 1
l’extérieur exterior
slightly curved légèrement courbé leicht einwärts ligeramente curvado Dwarf Cavendish 3
inwards vers l’intérieur gebogen hacia el interior
Example Varieties/
Exemples/ Note/
English français deutsch Español
Beispielssorten/ Nota
Variedades ejemplo
14. Leaf blade: color of Limbe : couleur de Blattspreite: Farbe Limbo: color de la
(*) midrib on lower side la nervure sur der Mittelrippe an nervadura en el envés
la face inférieure der Unterseite
15. Leaf blade: shape of Limbe : forme de Blattspreite: Form Limbo: forma de la
(*) base la base der Basis parte basal
(+)
PQ (a) both sides rounded deux bords arrondis beide Seiten ambos lados Bluggoe 1
abgerundet redondeados
one side rounded and un bord arrondi et eine Seite abgerundet un lado redondeado y Silk 2
one side acute un bord aigu und eine Seite spitz un lado agudo
both sides acute deux bords aigus beide Seiten spitz ambos lados agudos Grand Nain 3
QN (a) absent or very weak absente ou très faible fehlend oder sehr ausente o muy débil Sucrier 1
gering
Example Varieties/
Exemples/ Note/
English français deutsch Español
Beispielssorten/ Nota
Variedades ejemplo
17. Leaf blade: length Limbe : longueur Blattspreite: Länge Limbo: longitud
18. Leaf blade: width Limbe : largeur Blattspreite: Breite Limbo: anchura
QN (a) weakly elongated faiblement allongé schwach verlängert débilmente elongado Dwarf Cavendish 3
strongly elongated fortement allongé stark verlängert fuertemente elongado Branca, Sucrier 7
20. Leaf blade: Limbe : brillance de Blattspreite: Glanz Limbo: brillo del haz
(*) glossiness of upper la face supérieure der Oberseite
side
Example Varieties/
Exemples/ Note/
English français deutsch Español
Beispielssorten/ Nota
Variedades ejemplo
QN (b) absent or very weak absente ou très faible fehlend oder sehr ausente o muy débil Branca 1
gering
Example Varieties/
Exemples/ Note/
English français deutsch Español
Beispielssorten/ Nota
Variedades ejemplo
(+)
strongly turned up fortement relevé stark aufwärts fuertemente girado Figue Pomme, 3
gebogen hacia arriba IAC 2001, Terra,
Example Varieties/
Exemples/ Note/
English français deutsch Español
Beispielssorten/ Nota
Variedades ejemplo
curved with vertical courbé avec extrémité gekrümmt mit curvado con el extremo Branca, Gros Michel, 3
end verticale senkrechtem Ende vertical Lacatan
horizontal with horizontal avec horizontal mit horizontal con el Mysoure, Sucrier 4
inclined end extrémité inclinée geneigtem Ende extremo inclinado
QN (c) absent or weak absente ou faible fehlend oder gering ausente o débil IAC 2001, Silk, Sucrier 1
Example Varieties/
Exemples/ Note/
English français deutsch Español
Beispielssorten/ Nota
Variedades ejemplo
slightly curved in légèrement courbée leicht gebogen im ligeramente curvado en Lacatan, Nanicão 2
distal part dans la partie distale distalen Teil la parte distal
QN (c) absent or weak absentes ou faibles fehlend oder gering ausentes o débiles IAC 2001, Silk, 1
Sucrier,
Yangambi Km 5
38. Fruit: width Fruit : largeur Frucht: Breite (ohne Fruto: anchura
(*) (excluding ridges) (arêtes exclues) Rippen) (excluyendo las
(+) aristas)
Example Varieties/
Exemples/ Note/
English français deutsch Español
Beispielssorten/ Nota
Variedades ejemplo
39. Fruit: length of Fruit : longueur Frucht: Länge des Fruto: longitud del
pedicel du pédicelle Stiels pedicelo
(+)
40. Fruit: shape of apex Fruit : forme Frucht: Form der Fruto: forma del ápice
(*) du sommet Spitze
(+)
41. Fruit: thickness of Fruit : épaisseur de Frucht: Dicke der Fruto: espesor de la
(*) peel la peau Schale cáscara
(+)
Example Varieties/
Exemples/ Note/
English français deutsch Español
Beispielssorten/ Nota
Variedades ejemplo
42. Fruit: color of peel Fruit : couleur de Frucht: Farbe der Fruto: color de la
(*) (before maturity) la peau (avant Schale (vor der cáscara (antes de la
(+) maturité) Reife) madurez)
dark yellow jaune foncé dunkelgelb amarillo oscuro São Domingos, Sucrier 3
dark green vert foncé dunkelgrün verde oscuro Mysore, Sao Tome 7
43. Fruit: color of peel Fruit : couleur de Frucht: Farbe der Fruto: color de la
(*) la peau Schale cáscara
PQ (d) light yellow jaune clair hellgelb amarillo claro Branca, Gros Michel, 1
Pacovan
dark yellow jaune foncé dunkelgelb amarillo oscuro Pisang Mas, Silk, 5
Sucrier
red orange rouge orangé rotorange naranja rojizo Gren Red, São Tomé 7
Example Varieties/
Exemples/ Note/
English français deutsch Español
Beispielssorten/ Nota
Variedades ejemplo
46. Fruit: color of flesh Fruit : couleur de Frucht: Farbe des Fruto: color de la
(*) la chair Fruchtfleisches cáscara
Example Varieties/
Exemples/ Note/
English français deutsch Español
Beispielssorten/ Nota
Variedades ejemplo
51. Bract: color of inner Bractée : couleur de Deckblatt: Farbe Bráctea: color del
side la face interne der Innenseite envés
Example Varieties/
Exemples/ Note/
English français deutsch Español
Beispielssorten/ Nota
Variedades ejemplo
52. Bract: shape of apex Bractées : forme du Deckblatt: Form der Bráctea: forma del
sommet Spitze ápice
(+)
Characteristics containing the following key in the second column of the Table of
Characteristics should be examined as indicated below:
(a) Unless otherwise stated, all observations on the leaf should be made on the third
leaf from the apex at the moment of inflorescence emerging.
(b) Observations on the fruit bunch should be done at fruit maturity (harvest time).
(c) Observations on inflorescence and flower should be made at the time of full
flowering.
(d) Observations on the fruit should be made on the third hand on a median standard
fruit of the inner cluster, at stage 6 for ripe fruit.
suckers
Ad. 1: Ploidy
Used for musa:
Chromosome counts from root tips of plants. Briefly, fresh root tips were pretreated for
2 h in 0.036% 8-hydroxyquinoline and then fixed in 3:1 ethanol-acetic acid. The meristematic
zones were digested at 37° C in an enzyme mixture consisting of 5% cellulase (Sigma
Chemicals), 1% pectinase and 1% pectolyase Y23 (Karlan Research, Santa Rosa, Calif) made
in a citrate buffer, pH 4.5.The enzyme solution was removed and the meristems were washed
with water several times. A single meristem was placed on a glass slide, the excess water
removed with a paper towel and 1 or 2 drops of freshly prepared 3:1 ethanol-acetic acid
placed over it. The meristem was macerated and the cells smeared over the slide with a fine
forceps. The slide was observed in a phase contrast microscope. When the cells began to
adhere to the slide, several drops of the 3:1 was placed over one end of the slide and allowed
to flow over the cells. The slide was air-dried and stained with Leishman's stain as described
by Singh (1993).
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suckers
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The length of the pseudostem should be observed from the ground level to the crown of
the peduncle, at the beginning of flowering.
The diameter of the pseudostem should be observed at a consistent height above ground
level for all varieties (e.g. 0.3 meters above ground) at the beginning of flowering.
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1 2 3
absent or weak medium strong
3 5 7
weak medium strong
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3 7
loose compact
1 2 3
upright spreading drooping
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1 2 3
curved outwards straight slightly curved inwards
4 5
moderately curved inward overlapping
1 2 3
both sides rounded one side rounded and both sides acute
one side acute
The length of the peduncle should be determined from the attachment point of the
bunch to the first hand.
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The diameter of the peduncle should be assessed in the middle point between the
attachment point of the bunch and the first hand.
1 3 5 7
absent or very weak weak medium strong
The length of the bunch should be measured from the attachment point of the first hand
to the last hand.
The diameter of the bunch should be measured at the midpoint between the attachment
of the first hand and the attachment of the last hand.
1 2 3
cylindrical irregular conical
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1 2 3
horizontal to slightly turned up moderately turned up strongly turned up
hand
1 2 3 4
vertical inclined curved with vertical horizontal with
end inclined end
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1 3
weak strong
1 3
absent or weak strong
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1 2 3 4
straight slightly curved in evenly curved S-shaped
distal part
To be observed on the outer cluster of the third hand on the middle fruit.
1 2 3
absent or weak moderate strong
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The length of the fruit should be determined on the outer (convex) side from where the
fruit widens at the stalk end to the apical point.
Length of fruit
1 2 3 4
rounded truncate bottle-necked pointed
The color of the peel should be observed when the fruit has developed to its full size.
1 9
absent present
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1 9
absent present
Should be assessed in cross section at harvest time. Only for varieties with “Male
inflorescence: persistence: present”.
1 2 3 4
lanceolate narrow ovate medium ovate broad ovate
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1 2 3
closed or slightly open moderately open very open
1 2 3 4 5
narrow acute broad acute right angle obtuse emarginate
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9. Literature
Dadzie, B.K., Orchad, J. E., 1997: Routine Post Harvest Screening of Banana/Plantain
Hybrids: Criteria and Methods. INIBAP Technical Guidelines 2. International Plant Genetic
Resources Institute, Rome, Italy; International Network for the Improvement of Banana and
Plantain. Montpellier, FR
De Langhe, E., 1969: Bananas, Outlines of perennial crop breeding in the tropics.
Miscellaneous papers 4, Landbouwhogeschool, Wageningen, NL, pp. 53-78.
Descriptors for Banana [Musa spp] (revised). IBPGR/ICRISAT, Rome, IT, 1984.
Moreira, R. S., Saes, L. A., 1984: Considerações sobre o banco de germoplasma de banana
do IAC. In: Congresso brasileiro de fruticultura, 7. Anais. SBF, Florianópolis (SC), v.1:
220-236.
Moreira, R. S., Hiroce, R., Saes, L. A.: 1986: An Analysis of Twelve Nutrients in the
Internal and External Leaf Sample of Fifty Banana Cultivars. In: International Group on
Mineral Nutrition of Bananas, 3d meeting. Nelspruit. South Africa, August 1982. Fruits, v.
41(11): 669-673.
Moreira, R. S., 1999: Banana – Teoria e prática de cultivo. 2ª ed. São Paulo, SP: Fundação
Cargill, CD Rom nº 222, com 140 MB.
Pillay M., Hartman J., Dimkpa C., Makumbi D., 2003: Establishing the Genome of 'Sukali
Ndizi'. African Crop Science Journal, Vol. 11. No. 2, International Institute of Tropical
Agriculture, Eastern and Southern Africa Regional Center, Kampala, UG, pp. 119-124
Samson, J.A., 1980: Tropical Fruits. Longman. London, GB, pp. 133-138.
Silva, S.S., Shepherd, K., Dantas, J.L.L., Souza, A.S., Carneiro, M.S, 1999: Germoplasma.
In: Alves, E.J. (org.). A cultura da banana. 2. ed., rev. - Brasília-DF: Embrapa-SPI / Cruz das
Almas: Embrapa-CNPMF, pp. 61-84.
Simmonds, N.W., 1966: Bananas. 2nd ed., Longmans Green. London, GB, pp. 44-128.
Singh, R.J. 1993: Plant Cytogenetics. CRC Press, Inc., Boca Raton. 391 pp.
Stover, R.H., 1988: Variation and Cultivar Nomenclature in Musa, AAA Group, Cavendish
Subgroup. Fruits d'Outre-mer, Vol. 43, No. 6, FR, pp. 353-357.
Turner, D.W. and Hunt, N., 1984: Growth, yield and leaf nutrient composition of 30 banana
varieties in subtropical New South Wales. Dept. of Agriculture New South Wales, AU,
Technical Bulletin 31, pp. 1-36.
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Application date:
(not to be filled in by the applicant)
TECHNICAL QUESTIONNAIRE
to be completed in connection with an application for plant breeders’ rights
2. Applicant
Name
Address
Telephone No.
Fax No.
E-mail address
Breeder
(if different from applicant)
Breeder’s reference
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#
4. Information on the breeding scheme and propagation of the variety
4.1.1 Crossing
4.1.2 Mutation [ ]
(please state parent variety)
4.1.4 Other [ ]
(please provide details)
4.2.2 Seed [ ]
4.2.3 Other [ ]
(please provide details)
#
Authorities may allow certain of this information to be provided in a confidential section of the Technical
Questionnaire.
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long Pacovan 7[ ]
broad D’Angola, 7[ ]
Dwarf Cavendish,
IAC 2001
medium Dwarf Cavendish, 5[ ]
IAC 2001, Williams
orange 6[ ]
Comments:
#
7. Additional information which may help in the examination of the variety
7.1 In addition to the information provided in sections 5 and 6, are there any additional
characteristics which may help to distinguish the variety?
Yes [ ] No [ ]
(If yes, please provide details)
7.2 Are there any special conditions for growing the variety or conducting the examination?
Yes [ ] No [ ]
(If yes, please provide details)
#
Authorities may allow certain of this information to be provided in a confidential section of the Technical Questionnaire.
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(a) Does the variety require prior authorization for release under legislation
concerning the protection of the environment, human and animal health?
Yes [ ] No [ ]
Yes [ ] No [ ]
9.2 The plant material should not have undergone any treatment which would affect the
expression of the characteristics of the variety, unless the competent authorities allow or
request such treatment. If the plant material has undergone such treatment, full details of the
treatment must be given. In this respect, please indicate below, to the best of your knowledge,
if the plant material to be examined has been subjected to:
……………………………………………………………
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10. I hereby declare that, to the best of my knowledge, the information provided in this form
is correct:
Applicant’s name:
Signature Date:
[End of document]