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    Granucult™ VRB (Violet Red Bile Lactose) Agar Acc. Iso 4832 and Fda-Bam

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    Descripcion de agar rojo violeta de Merck

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    Granucult™ VRB (Violet Red Bile Lactose) Agar Acc. Iso 4832 and Fda-Bam

    Uploaded by

    noe
    72 views4 pages
    Descripcion de agar rojo violeta de Merck

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    Merck Rebrand_101406_1906 (1)

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    Descripcion de agar rojo violeta de Merck

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    72 views4 pages

    Granucult™ VRB (Violet Red Bile Lactose) Agar Acc. Iso 4832 and Fda-Bam

    Uploaded by

    noe
    Descripcion de agar rojo violeta de Merck

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 4

    Technical Data Sheet

    GranuCult™ VRB (Violet Red Bile Lactose) Agar


    acc. ISO 4832 and FDA-BAM
    Ordering number: 1.01406.0500 / 1.01406.5000

    For the detection and colony counting of coliform bacteria from food and animal feed, water
    and other materials.
    This culture medium complies with the specifications given by ISO 4832, FDA-BAM and APHA.
    Violet Red Bile (VRB) agar is also called Violet Red Bile Lactose (VRBL) agar or Crystal Violet
    Neutral red Bile Lactose agar.
    Mode of Action
    Crystal violet and bile salts inhibit growth primarily of the Gram-positive accompanying
    bacterial flora. Degradation of lactose to acid is indicated by the pH indicator neutral red which
    changes its color to red and by precipitation of bile acids. Enzymatic digest of animal tissue
    provides carbon and nitrogen sources for the growth and yeast extract primarily supplies
    the B-complex vitamins whilst agar is the solidifying agent.
    Typical Composition

    GranuCultTM VRB Agar


    acc. ISO 4832 and
    Specified by ISO 4832 FDA-BAM M174
    FDA-BAM

    Enzymatic
    Enzymatic Digest Peptone or
    7 g/l 7 g/l Digest of Animal 7 g/l
    of Animal Tissues Gelysate
    Tissues
    Yeast Extract 3 g/l Yeast Extract 3 g/l Yeast Extract 3 g/l
    Bile Salts or Bile
    Bile Salts 1.5 g/l 1.5 g/l Bile Salts 1.5 g/l
    Salts No. 3
    NaCl 5 g/l NaCl 5 g/l NaCl 5 g/l
    Lactose 10 g/l Lactose 10 g/l Lactose 10 g/l
    Neutral Red 0.03 g/l Neutral Red 0.03 g/l Neutral Red 0.03 g/l
    Crystal Violet 0.002 g/l Crystal Violet 0.002 g/l Crystal Violet 0.002 g/l
    Agar 12-18 g/l Agar 15 g/l Agar-Agar* 13 g/l
    Water 1000 ml/l Water 1000 ml/l Water n/a
    pH at 25 °C 7.2 ± 0.2 pH at 25 °C 7.2 ± 0.2 pH at 25 °C 7.2 ± 0.2

    * Agar-agar is equivalent to other different terms of agar.

    1.01406.0500 / 1.01406.5000 Page 1 of 4


    Preparation

    Dissolve 39.5 g in 1 l of purified water. Heat in boiling water and agitate frequently until
    completely dissolved. Afterwards do not boil for more than 2 minutes.

    Do not autoclave. Do not overheat!

    If the medium is to be used immediately for poured plate technique, cool it to 44-47 °C in a
    water bath before use. Use the molten medium as soon as possible, it should not be retained
    for more than 4 h, as specified by ISO 4832 and EN ISO 11133.

    If the medium is used for surface plating technique, there should be no visible moisture on
    the plates before use. When moisture is present, the plates should be dried for the
    minimum time required to remove visible moisture, following the procedure as described by
    EN ISO 11133.

    The prepared medium is clear and red. The pH value at 25 °C is in the range of 7.0-7.4.

    Experimental Procedure and Evaluation

    Depend on the purpose for which the medium is used. VRB agar is usually inoculated by
    poured plate techniques, e.g. as described by ISO 4832.

    Incubate the inoculated plates under aerobic conditions. e.g. acc. to EN ISO 4832 at 29-31 °
    C or at 36-38 °C for 22-26 h.

    Coliform bacteria will produce purplish-red colonies of at least 0.5 mm diameter, sometimes
    surrounded by a reddish zone of precipitated bile. These are considered as typical colonies of
    coliforms and do not require further confirmation.

    The appearance of a reddish zone of precipitated bile around the colonies depends on the
    type of coliforms.

    Atypical colonies (e.g. smaller size than 0.5 mm) should be counted and confirmed, e.g.
    following the procedure as described by ISO 4832 by using BRILA broth (article number
    1.05454.0500).

    When testing products containing sugars other than lactose, presence of these sugars may
    lead to false positive results, in which case colonies should be confirmed.

    Storage

    Store at +15 °C to +25 °C, dry and tightly closed. Do not use clumped or discolored
    medium. Protect from UV light (including sun light). For in vitro use only.

    According to Corry et al. (2012), self-prepared plates can be stored at +2 °C to +8 °C in


    the dark and protected against evaporation for up to 5 days.

    1.01406.0500 / 1.01406.5000 Page 2 of 4


    Quality Control

    Reference Method of Expected


    Function Control strains Incubation
    medium control results
    Escherichia Recovery ≥
    coli ATCC® 50 %,
    8739 22-26 h at purplish-red
    Escherichia Tryptic Soy
    Productivity 29-31 ° Quantitative colonies with
    coli ATCC® Agar (TSA)
    C or without
    25922
    Enterobacter aerobic precipitation
    cloacae ATCC® halo
    13047
    Enterococcus
    22-26 h at
    faecalis ATCC® Total
    Selectivity 29-31 °C - Qualitative
    19433
    Enterococcus inhibition
    aerobic
    faecalis ATCC®
    29212 No limit for
    recovery
    Pseudomona 22-26 h at
    specified,
    Specificity s aeruginosa 29-31 ° - Qualitative
    colorless to
    ATCC® 27853 C
    beige
    aerobic
    colonies

    Please refer to the actual batch related Certificate of Analysis.

    The performance test is in accordance with the current version of EN ISO 11133. A

    recovery rate of 50 % is equivalent to a productivity value of 0.5.

    Escherichia Salmonella typhimurium


    coli ATCC® ATCC® 14028
    11775

    1.01406.0500 / 1.01406.5000 Page 3 of 4


    Literature

    APHA (2015) Compendium of Methods for the Microbiological Examination of Foods. 5th ed.
    American Public Health Association, Washington, D.C.

    APHA (2004) Standard Methods for the Examination of Dairy Products. 17th ed. American
    Public Health Association, Washington, D.C.

    Corry, J.E.L., Curtis, G.D.W. and Baird, R.M. (2012): Handbook of Culture Media for Food
    and Water Microbiology, pp. 959 - 961. Royal Society of Chemistry, Cambridge, UK.

    FDA-BAM (2002): Chapter No. 4: Enumeration of Escherichia coli and the Coliform Bacteria.
    U.S. Food and Drug Administration - Bacteriological Analytical Manual.

    ISO International Standardisation Organisation. Microbiology of food and animal


    feeding stuffs -- Horizontal method for the enumeration of coliforms - Colony-count
    technique. ISO 4832:2006.

    ISO International Standardisation Organisation. Microbiology of food, animal feed


    and water - Preparation, production, storage and performance testing of culture media.
    EN ISO 11133:2014.

    Ordering Information

    Product Cat. No. Pack size


    GranuCult™ VRB (Violet Red Bile Lactose)
    1.01406.0500 500 g
    Agar acc. ISO 4832 and FDA-BAM
    GranuCult™ VRB (Violet Red Bile Lactose)
    1.01406.5000 5 kg
    Agar acc. ISO 4832 and FDA-BAM
    ReadyTube™ 200 VRBL Agar 1.46423.0006 6 x 200 ml bottles
    GranuCult™ BRILA (Brilliant-Green Bile Lactose)
    1.05454.0500 500 g
    Broth acc. ISO 4831, ISO 4832 and FDA-BAM
    GranuCult™ BRILA (Brilliant-Green Bile Lactose)
    1.05454.5000 5 kg
    Broth acc. ISO 4831, ISO 4832 and FDA-BAM

    Merck KGaA Find contact information for your


    Frankfurter Strasse 250 64293 country at:
    Darmstadt, Germany Fax: +49 www.merckmillipore.com/offices
    (0) 61 51 / 72-60 80
    For Technical Service, please visit:
    www.merckmillipore.com/techservice
    For more information, visit
    www.merckmillipore.com/biomonitoring

    GranuCult, Merck, Millipore, and Sigma-Aldrich are trademarks of Merck KGaA,


    Darmstadt, Germany or its affiliates. Detailed information on trademarks is available
    via publicly accessible resources.
    © 2019 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

    1.01406.0500 / 1.01406.5000 Page 4 of 4

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