Int. J. Pharm. Sci. Rev. Res., 62(2), May - June 2020; Article No.

07, Pages: 37-39 ISSN 0976 – 044X

Research Article

Formulation and Evaluation of Herbal Hand Sanitizer Using Psidium guajava Leaves Extract

1
Firdous Shaikh*, 2Priyanka Bhosale, 3Snehal More
1 Department of Pharmaceutics, 2Department of Pharmacognosy, 3Department of Pharmaceutics
Satara College of Pharmacy, Satara (Maharashtra), India.
*Corresponding author’s E-mail: [email protected]

Received: 17-03-2020; Revised: 22-05-2020; Accepted: 30-05-2020.

ABSTRACT
This research paper is centered on the effectiveness, bringing to light an optimistic effect of herbal hand sanitizer using Psidium
guajava leaves extract. A large portion of the research has focused on hygiene by controlling the entry of pathogens into the body
through hands. Having run over the positive advantages on reducing the microbes, the aim for the current study is set up. Natural
herbal hand sanitizers are effective, environment friendly, and biodegradable, inexpensive.
Keywords: Hand Sanitizer, Hygiene, Pathogens, Psidium guajava.

INTRODUCTION MATERIALS AND METHODS

H ands are primary mode of spread of microbes

and infections. Hand hygiene is thus the most
vital measure to evade the transmission of
harmful germs and avoid the infections. Hand hygiene
is the single most important, simplest, and least pricey
Extraction procedure for Guava leaves:
1. 50gm of fresh leaves of guava (Psidium guajava)
were collected.
2. Leaves were cut into small pieces.
means of preventing nosocomial infections.1
3. 50 gm of the leaves were soaked in 95% ethanol for
Contaminated hand can give out vectors for the
3 to 4 days.
transmission of microorganisms. Pathogenic
microorganisms responsible for epidemics are spread 4. The extract was filtered with Whatman filter paper
from the hands of the food handler to others, when the & the filtrate was collected. 5
food handler contaminates his/her hands and then
Table 1: Formulation Table
passes these microorganisms to consumers by means
of hand contact with food or drinks. The consumer is Sr. No. Ingredients Quantity
bared following the intake of these microorganisms,
1. Carbopol 0.18 gm
which may cause gastrointestinal illness. Hand contact
with ready-to-eat foods symbolizes a very important 2. Alcohol 18.6 ml
means by which pathogens may enter the food supply. 3. Glycerin 0.69 ml
2
To guard the skin from harmful micro organisms and
to prevent spreading of many communicable diseases, 4. Methyl paraben 0.5 gm
hand washing is absolutely an important safeguard. 3 5. Guava leaves extract 0.5 gm
Before the invention of contemporary medicine, plants 6. Triethanolamine 0.07 ml
were the chief remedy for treating various diseases. 7. Deionised water 9.3 ml
With the arrival of different antibiotics, microbes also
slowly develop resistance to these substances. These 8. Perfume 0.15 ml
bring researchers importance towards the plants Procedure for formulation of Hand Sanitizer:
having antimicrobial properties. They endeavour to
develop the inimitable ability of different secondary 1. Carbopol was added to deionised water with
metabolites to show constant and long-lasting activity constant stirring.
against broad spectrum of microbes. 4 2. After uniform mixing, Triethanolamine was added
Thus, in this research study, we used Psidium guajava with slow stirring to avoid formation of possible air
leaves extract to develop a hand sanitizer due to its bubble in the product.
beneficial effect against pathogenic microbes. 3. Kept aside for 24 hrs.

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Int. J. Pharm. Sci. Rev. Res., 62(2), May - June 2020; Article No. 07, Pages: 37-39 ISSN 0976 – 044X

4. The extract of guava leaves and Carbopol were two plates were kept for incubation for 24 hrs at 370 C.
added to denaturing alcohol with glycerin, Poly After the incubation period, the zone of inhibition was
sorbate 20, were mixed with aqueous phase. found on the petri plate. 7
5. Finally, methyl paraben was added as a The zone of inhibition of herbal hand sanitizer and pure
preservative and perfume was added. form of antibiotic Gentamycin was appeared. In image A,
the zone of inhibition of E. coli was appeared and in image
6. Mixed with slow stirring to obtain uniform
B the zone of inhibition of Bacillus subtilis was seen. Result
product. 6
of antibacterial test of herbal hand sanitizer against
Evaluation Parameters: standard of pure antibiotic Gentamycin was measured in
mm by scale.
1. Organoleptic Properties:-Tests like Colour, Odour
and clarity were carried out. RESULTS AND DISCUSSION
2. Physical properties:- RESULTS
a) pH: The pH was determined by using pH paper. Evaluation Parameters:
b) Irritancy test: 5 healthy volunteers were selected. 1. Organoleptic Evaluation:
The herbal hand sanitizer was applied on palm and time
1. Colour: - Green
was noted. Irritancy, redness, dryness and itching were
checked. 2. Odour: - Characteristics
c) Evaporation rate: 5 healthy volunteers were 3. Clarity: - Opaque
selected. The herbal hand sanitizer was applied on their
2. Physical properties:
palm while rubbing the sanitizer on palm. Evaporation
took place and that time was noted. Evaporation rate a) pH: - 4 to 6
was below 1 min.
b) Irritancy test:- No irritancy
d) Antibacterial Test: The antibacterial activity of
herbal hand sanitizer using different solvents against
strains of aerobic and anaerobic micro-organism was
evaluated by standard cup plate method. For this
standard cup plate method, the nutrient agar medium
was used as a culture media.
Table 2: Composition of Agar medium
Sr. Ingredients Quantity
No. (gm)
1. Peptic digest of animal tissue 5 Figure 1: Irritancy test
2. Sodium chloride 5 In this particular evaluation test, the antibacterial of
3. Beef extract 1.50 herbal hand sanitizer was found to be safe and on the
basis of this information, we can say that the herbal
4. Yeast extract 1.50
hand sanitizer shows somewhat lesser activity than
5. Agar 8.5 pure antibiotic Gentamycin. But, definitely the
To perform antibacterial test, the pre-sterilized petri formulated herbal hand sanitizer has anti-microbial
plate was used. To pre-sterilize, the petri plate was activity against bacterial species like E. coli and Bacillus
incubated for 24 hrs at 37˚C. Then next to this, the agar subtilis.
culture media was poured on the petri plate uniformly
in aseptic condition. After spreading, the agar medium
was covered with another petri plate and kept aside for
24 hrs in refrigerator to solidify the agar medium. After
this, the plate was removed and on these plates the cup
was formed. On two particular plates, the standard
solution which contains microorganism strains i.e. E.
coli and Bacillus subtilis were uniformly spread in
aseptic condition. Now, in which the cup was formed
on two petri plates, the standard which is pure
antibiotic Gentamycin; 1ml was added by the pipette
and in the next cup, the formulated herbal hand
sanitizer was added in aseptic condition. Then these Figure 2: Image A (E. coli)

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Int. J. Pharm. Sci. Rev. Res., 62(2), May - June 2020; Article No. 07, Pages: 37-39 ISSN 0976 – 044X

evaporates usually requires only 15 to 30 seconds.

Whereas vigorous friction, rinsing with water, and drying
with a towel are not needed like hand disinfectants or
soaps. 8
CONCLUSION
Hands are the most common mode of transmission of
pathogens to patients and proper hand hygiene can
prevent health care-associated infections and the spread
of antibacterial resistance. Scientific proof and easiness of
use support alcohol-based hand sanitizers during patient
care. It may be concluded that herbal hand sanitizer has a
significant bacterial effect on the specified
Figure 3: Image B (B. subtilis) microorganisms. Thus, there is immense potential in
establishing the use of antibacterial herbal products as a
Table 3: Results measure to control the multidrug resistant microbes as
Sr. Parameters Results well as check their spread through hands from one
No. geographical region to another. Herbal hand sanitizer is
based alternative for chemically prepared containing
1. Organoleptic properties Green
active silver nitrates. Natural herbal hand sanitizers are
2. a) Colour Characteristic effective, environment friendly, and biodegradable,
3. b) Odour Opaque inexpensive.
4. c) Clarity 4 to 6 REFERENCES
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Source of Support: Nil, Conflict of Interest: None.

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