Lab Write Up
Lab Write Up
Hour 5
12-21-10
solution of Bromothymol Blue (C27H28Br2O5S) has a pH range of 6.0 to 7.6. When Carbon Dioxide is
released from the pond snail through cell respiration into the solution, the Bromothymol Blue will slowly
reach a pH balance of 7.0, this occurs when the mixture is neutral and the color present is Green
Dioxide from the pond snail is added to Bromothymol when the solution is Green or Neutral (pH of 7.0)
then it will produce Carbonic Acid and turn the Bromothymol Blue to a yellow color due to the Acid
produced through Photosynthesis from the Elodea, then the pH balance will increase toward 7.0 or
neutral again.
Step 2: Hypothesis:
If the Elodea, which is being placed in light, gives off Oxygen, Then the snail will use this oxygen
and go through cell respiration, because the outputs of Photosynthesis (C6H12O6 + 6O2) are the inputs for
Cellular Respiration and the outputs of Cellular Respiration (6CO2 + 6H2O + Energy) are the inputs for
Photosynthesis.
Dependent Variables: The Color of the Bromothymol Blue solution because it depends on the
Step 4: Constants
Temperature
Air Pressure
Time
Type of Elodea
Amount of light
If the Hypothesis is correct, the test tube of only the snail will turn the Bromothymol yellow due
to the Carbonic Acid it should produce, the test tube of only the Elodea will keep the
Bromothymol blue do to the Oxygen it is producing and the test tube of the Snail and Elodea will
turn the Bromothymol Green do the equalization of the Carbon Dioxide and Oxygen being
Step 6: Procedure
2. Put the 30mL of Water (H2O) into a test tube (Repeat 3 more times)
3. Place 15 drops of Bromothymol Blue into each of the four test tubes
4. In test tube one, leave it as it is, this will be our Control experiment
5. In test tube two, add one pond snail into the test tube
6. In test tube three, add one piece of Elodea into the test tube
7. In test tube four, add one pond snail and one piece of Elodea into the test tube
10. After the 24 hours is over, observe the test tubes, record the colors of the test tubes, put
tubes, put them once more into a light environment for a final 24 hours
12. After the period of 24 hours is complete, observe the test tubes, record the colors of the
test tubes.
Elodea
Color of Bromothymol Blue Light Blue Light Blue
Elodea
Color of Bromothymol Blue Green Green
Blue for Elodea only
Hours 24 48 72
This data shows that in a light environment, the color of the Bromothymol blue only had a color
change when the test tube consisted of Elodea. This is also true for the data from the Dark
Environment table; they experienced the same aspect as we did. This was when the Elodea was
either alone or with the pond snail, The Bromothymol blue changed colors. In both cases, there
was no difference in the test tube with only the pond snail, it remained blue.
Step 9: Conclusion
In the experiment the purpose was to find if the Bromothymol blue would change when
different variables (snail, Elodea, snail and Elodea) were added to the test tube and observed
over 72 hours. The results mostly did not support the hypothesis because of the data we
observed. In the hypothesis, the prediction was that the test tube with the snail would turn the
Bromothymol into a yellow due to the Carbonic acid it would produce. Also, the end result was
the same from the beginning which meant that the solution did not change color at all. However
when the Elodea and snail were added to the test tube, we predicted that the Bromothymol
would turn green. After the 72 hours of observations, both test tubes in both experiments
turned to either a green or a near green. This observation did support the hypothesis despite
Based on the results we gathered from the experiments, there was no significant difference
between the experiment that took place in light and the one that took place in the dark. We believe that
the snail inside the test tube with only Bromothymol died during the process. We believe this because
after 72 hours of observations, there was no color difference in both experiments. This led our
hypothesis to be partially incorrect. However, both groups did get the same result for the test tube with
the Elodea and that was a color change to Green, Both of switch we predicted. It turned green do to the
oxygen the Elodea produces through Photosynthesis. When the Elodea and the pond snail were added,
both light and dark groups the solution turned into a green color. This is due to the equalization of
Photosynthesis by the Elodea and the Cellular Respiration by the pond snail. In a future experiment, we
would do two experiments in either light or dark because that would give a better indication on what
"Bromthymol Blue, 30ml (1 Oz.) for Sale. Buy from the Science Company." The Science
Company Lab Supplies, Chemicals, Microscopes and Weather Instruments. Web. 18 Dec. 2010.
<http://secure.sciencecompany.com/Bromthymol-blue-30ml-1-oz-P6363.aspx>.
<http://antoine.frostburg.edu/chem/senese/101/acidbase/indicators.shtml>.
"Bromothymol Blue: Information from Answers.com." Answers.com: Wiki Q&A Combined with
<http://www.answers.com/topic/bromothymol-blue-1>.