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The document describes an experiment testing the effects of a snail, Elodea, and their combination on the color of a Bromothymol Blue solution over time. It was hypothesized that the snail alone would acidify the solution and turn it yellow, Elodea alone would keep it basic and blue, and their combination would keep it neutral and green. The results partially supported this, with Elodea changing the solution color but not the snail alone, likely because the snail died. Both light and dark trials produced similar results, with the Elodea and combination turning the solution green.
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0% found this document useful (0 votes)
2K views6 pages

Lab Write Up

The document describes an experiment testing the effects of a snail, Elodea, and their combination on the color of a Bromothymol Blue solution over time. It was hypothesized that the snail alone would acidify the solution and turn it yellow, Elodea alone would keep it basic and blue, and their combination would keep it neutral and green. The results partially supported this, with Elodea changing the solution color but not the snail alone, likely because the snail died. Both light and dark trials produced similar results, with the Elodea and combination turning the solution green.
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You are on page 1/ 6

Christian Roemhildt

Hour 5

12-21-10

Step 1: Background Information:

According to http://secure.sciencecompany.com/Bromthymol-blue-30ml-1-oz-P6363.aspx, the

solution of Bromothymol Blue (C27H28Br2O5S) has a pH range of 6.0 to 7.6. When Carbon Dioxide is

released from the pond snail through cell respiration into the solution, the Bromothymol Blue will slowly

reach a pH balance of 7.0, this occurs when the mixture is neutral and the color present is Green

(http://antoine.frostburg.edu/chem/senese/101/acidbase/indicators.shtml). However, if more Carbon

Dioxide from the pond snail is added to Bromothymol when the solution is Green or Neutral (pH of 7.0)

then it will produce Carbonic Acid and turn the Bromothymol Blue to a yellow color due to the Acid

being produced. http://www.answers.com/topic/bromothymol-blue-1. However, when oxygen is

produced through Photosynthesis from the Elodea, then the pH balance will increase toward 7.0 or

neutral again.

Step 2: Hypothesis:

If the Elodea, which is being placed in light, gives off Oxygen, Then the snail will use this oxygen

and go through cell respiration, because the outputs of Photosynthesis (C6H12O6 + 6O2) are the inputs for

Cellular Respiration and the outputs of Cellular Respiration (6CO2 + 6H2O + Energy) are the inputs for

Photosynthesis.

Step 3: Experimental Design


(BB-Bromothymol Blue)

Experimental Set-up Control Set-up

Four Test tubes – One One test tube of only

test tube has a Snail with Bromothymol Blue

BB, Second test tube has

Elodea and BB, Third Test

tube has a Snail, Elodea

and BB. Fourth test tube

only has BB and this will

be the control variable

Independent Variables: The snails and Elodea in the test tubes

Dependent Variables: The Color of the Bromothymol Blue solution because it depends on the

amount of Carbon Dioxide released.

Control: A test tube with only Bromothylmol Blue

Step 4: Constants

 Temperature

 Air Pressure

 Amount of Bromothymol Blue

 Time

 Type of Pond Snails

 Type of Elodea
 Amount of light

 Test tube Size/Volume

 Gravitational Pull on test tubes

Step 5: Prediction of Results

If the Hypothesis is correct, the test tube of only the snail will turn the Bromothymol yellow due

to the Carbonic Acid it should produce, the test tube of only the Elodea will keep the

Bromothymol blue do to the Oxygen it is producing and the test tube of the Snail and Elodea will

turn the Bromothymol Green do the equalization of the Carbon Dioxide and Oxygen being

produced by both subjects.

Step 6: Procedure

1. Measure 30mL of Water (H2O) In a graduated cylinder (Repeat 3 more times)

2. Put the 30mL of Water (H2O) into a test tube (Repeat 3 more times)

3. Place 15 drops of Bromothymol Blue into each of the four test tubes

4. In test tube one, leave it as it is, this will be our Control experiment

5. In test tube two, add one pond snail into the test tube

6. In test tube three, add one piece of Elodea into the test tube

7. In test tube four, add one pond snail and one piece of Elodea into the test tube

8. Put a cover on all four test tubes

9. Leave in a light environment for 24 hours

10. After the 24 hours is over, observe the test tubes, record the colors of the test tubes, put

them again in a light environment for 24 years hours


11. After another 24 hours is finished, observe the test tubes, record the colors of the test

tubes, put them once more into a light environment for a final 24 hours

12. After the period of 24 hours is complete, observe the test tubes, record the colors of the

test tubes.

13. Clean up the lab

Step 7: Process of Data

Experiment in Light Environment


Color of Bromothymol Blue Blue Blue

Blue for control


Color of Bromothymol Blue Blue Blue

Blue for Snail only


Color of Bromothymol Light Blue Blue/Green Blue/Green

Blue for Snail and

Elodea
Color of Bromothymol Blue Light Blue Light Blue

Blue for Elodea only


Hours 24 48 72

Experiment in Dark Environment


Color of Bromothymol Blue Blue Blue

Blue for control


Color of Bromothymol Blue Blue Blue

Blue for Snail only


Color of Bromothymol Light Blue Dark Blue/Green Dark Blue/Green

Blue for Snail and

Elodea
Color of Bromothymol Blue Green Green
Blue for Elodea only
Hours 24 48 72

Step 8: Interpretation of Results

This data shows that in a light environment, the color of the Bromothymol blue only had a color

change when the test tube consisted of Elodea. This is also true for the data from the Dark

Environment table; they experienced the same aspect as we did. This was when the Elodea was

either alone or with the pond snail, The Bromothymol blue changed colors. In both cases, there

was no difference in the test tube with only the pond snail, it remained blue.

Step 9: Conclusion

In the experiment the purpose was to find if the Bromothymol blue would change when

different variables (snail, Elodea, snail and Elodea) were added to the test tube and observed

over 72 hours. The results mostly did not support the hypothesis because of the data we

observed. In the hypothesis, the prediction was that the test tube with the snail would turn the

Bromothymol into a yellow due to the Carbonic acid it would produce. Also, the end result was

the same from the beginning which meant that the solution did not change color at all. However

when the Elodea and snail were added to the test tube, we predicted that the Bromothymol

would turn green. After the 72 hours of observations, both test tubes in both experiments

turned to either a green or a near green. This observation did support the hypothesis despite

other observations which did not.

Step 10: Analysis

Based on the results we gathered from the experiments, there was no significant difference

between the experiment that took place in light and the one that took place in the dark. We believe that
the snail inside the test tube with only Bromothymol died during the process. We believe this because

after 72 hours of observations, there was no color difference in both experiments. This led our

hypothesis to be partially incorrect. However, both groups did get the same result for the test tube with

the Elodea and that was a color change to Green, Both of switch we predicted. It turned green do to the

oxygen the Elodea produces through Photosynthesis. When the Elodea and the pond snail were added,

both light and dark groups the solution turned into a green color. This is due to the equalization of

Photosynthesis by the Elodea and the Cellular Respiration by the pond snail. In a future experiment, we

would do two experiments in either light or dark because that would give a better indication on what

one end of the result should be corrected too.

Step 11: Bibliography

"Bromthymol Blue, 30ml (1 Oz.) for Sale. Buy from the Science Company." The Science

Company Lab Supplies, Chemicals, Microscopes and Weather Instruments. Web. 18 Dec. 2010.

<http://secure.sciencecompany.com/Bromthymol-blue-30ml-1-oz-P6363.aspx>.

Senese, Fred. "Acid-Base Indicators." 10 Dec. 2001. Web. 18 Dec. 2010.

<http://antoine.frostburg.edu/chem/senese/101/acidbase/indicators.shtml>.

"Bromothymol Blue: Information from Answers.com." Answers.com: Wiki Q&A Combined with

Free Online Dictionary, Thesaurus, and Encyclopedias. Web. 19 Dec. 2010.

<http://www.answers.com/topic/bromothymol-blue-1>.

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