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    Objective: in This Virtual Lab, You Will Identify The Resources and Process of DNA Extraction

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    videet
    1. This virtual lab document outlines the steps and objectives of a DNA extraction lab. The goal is to isolate DNA from cheek cells for purposes like genetic testing and forensic analysis. The main steps involve using lysis solution to burst open cells and release DNA, salt solution and centrifugation to separate DNA from other cell debris, and alcohol precipitation to isolate purified DNA. Proper centrifuge balancing is important to avoid disrupting the separation process. Relating it to hair washing, shampoo performs a similar function of removing contaminants from hair and cell membranes.

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    Objective: in This Virtual Lab, You Will Identify The Resources and Process of DNA Extraction

    Uploaded by

    videet
    55 views2 pages
    1. This virtual lab document outlines the steps and objectives of a DNA extraction lab. The goal is to isolate DNA from cheek cells for purposes like genetic testing and forensic analysis. The main steps involve using lysis solution to burst open cells and release DNA, salt solution and centrifugation to separate DNA from other cell debris, and alcohol precipitation to isolate purified DNA. Proper centrifuge balancing is important to avoid disrupting the separation process. Relating it to hair washing, shampoo performs a similar function of removing contaminants from hair and cell membranes.

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    VIRTUAL_DNA_EXTRACTION_LAB

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    1. This virtual lab document outlines the steps and objectives of a DNA extraction lab. The goal is to isolate DNA from cheek cells for purposes like genetic testing and forensic analysis. The main steps involve using lysis solution to burst open cells and release DNA, salt solution and centrifugation to separate DNA from other cell debris, and alcohol precipitation to isolate purified DNA. Proper centrifuge balancing is important to avoid disrupting the separation process. Relating it to hair washing, shampoo performs a similar function of removing contaminants from hair and cell membranes.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    55 views2 pages

    Objective: in This Virtual Lab, You Will Identify The Resources and Process of DNA Extraction

    Uploaded by

    videet
    1. This virtual lab document outlines the steps and objectives of a DNA extraction lab. The goal is to isolate DNA from cheek cells for purposes like genetic testing and forensic analysis. The main steps involve using lysis solution to burst open cells and release DNA, salt solution and centrifugation to separate DNA from other cell debris, and alcohol precipitation to isolate purified DNA. Proper centrifuge balancing is important to avoid disrupting the separation process. Relating it to hair washing, shampoo performs a similar function of removing contaminants from hair and cell membranes.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 2

    NAME Videet Mehta Date 11/11/20 Period 7

    VIRTUAL DNA EXTRACTION LAB

    Objective: In this virtual lab, you will identify the resources and process of DNA Extraction.

    Introduction:

    1. Name two (2) reasons scientists isolate DNA.


    Genetic Testing
    Body Identification
    Analyze forensic evidence

    Step One: Collect Cheek Cells

    2. What is contained inside the nucleus of every cheek cell?

    DNA

    Step Two: Burst Cells Open to Release DNA

    3. “Lysis” is a Greek word that means what? __To separate__

    4. The Lysis solution you added to your Eppendorf tube and placed in the warm water bath contained detergent and
    enzymes.

    a) What was the purpose of the detergent?

    Disrupts the cell membrane and nuclear envelope, causing the cells to burst open and release their DNA

    b) What was the purpose of the proteinase K?

    This enzyme cuts apart the histones to free the DNA

    Step Three: Separate DNA from Proteins and Debris

    5. What is the function of the salt solution?

    The salt causes proteins and other cellular debris to clump together

    6. What is the function of the centrifuge step?

    The heavy clumps of protein and cellular debris sink to the bottom while the strands of DNA stay at the top.

    Step Four: Isolate Concentrated DNA

    7. Isopropyl alcohol is used to precipitate out the DNA because it is not soluble. What does this mean?

    The DNA will come out of the solution because DNA is not soluble

    8. Why must the new tube be centrifuged?

    The DNA goes to the bottom and we can extract the remaining liquid.

    Analysis and Conclusions

    9. Why do you think it is important to have a “balanced” centrifuge?


    NAME Videet Mehta Date 11/11/20 Period 7
    It can mess up the test tubes. In addition, if it is not balanced, it will have an irregular spin and will mess up the solution.

    10. Relate how shampoo performs the same function on your hair as it does on cell membranes in this lab.

    Shampoo takes away the oil and dirt from your hair just like how it takes away the DNA from the cells.

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