International Journal of Trend in Scientific Research and Development (IJTSRD)

Volume 4 Issue 6, September-October 2020 Available Online: www.ijtsrd.com e-ISSN: 2456 – 6470

The Integration of Flax Seed into Brewing and

Production of Omega-3S Enriched Beer
Durga Dharshini. D, Shirley. D, Abinaya. M
Students of Department of Food Technology,
JCT College of Engineering and Technology, Coimbatore, Tamil Nadu, India

ABSTRACT How to cite this paper: Durga Dharshini.

Brewing is a kind of art that should be done with passion and perfection. D | Shirley. D | Abinaya. M "The
Brewing holds a longest history ever to look after. We all may aware that the Integration of Flax Seed into Brewing and
most consumed and oldest brewing recipe is beer. Beer is nothing but a drink Production of Omega-3S Enriched Beer"
made of fermentation of starch rich barley in water which results in a sweet Published in
beverage with yeast. It provides most convincing and replenishing mouth feel. International Journal
Beer processing being in process since 5000 BC. There were 'n' number of of Trend in Scientific
varieties or flavours of beer you can spend your leisure time with. Now We are Research and
likely to introduce flax seeds into brewing integral to add some extra Development
nourishment to the world’s favorite drink. Brewing of flax seeds after proper (ijtsrd), ISSN: 2456-
processing gives you a unique mouth feel rather than that of a regular beer 6470, Volume-4 | IJTSRD33550
provides to your taste buds flax seeds contain bag full of nutrients. They are Issue-6, October
loaded with Omega 3 fatty acids and with some major vitamins and minerals. 2020, pp.1302-1307, URL:
Therefore brewing of flax seeds serves both on taste and on diet, now you www.ijtsrd.com/papers/ijtsrd33550.pdf
don't need to cheat on your diet to have a tasty beer. Let's have a look over the
processing of beer from nutritious flax seed. Copyright © 2020 by author(s) and
International Journal of Trend in Scientific
KEYWORDS: Beer-flaxseeds-brewing-hobs-fermentation-starch-beverages- Research and Development Journal. This
alcohol-temperature-barley-rice-tradition-neolethic-daily consumption-nutrition- is an Open Access article distributed
refreshment under the terms of
the Creative
Commons Attribution
License (CC BY 4.0)
(http://creativecommons.org/licenses/by/4.0)

INTRODUCTION
Since early times beer production was in practice. Beer have
the longest history among the traditional beverages. It was
said to be in practice during the Neolithic period. The oldest
beer was found in Mesopotamia in South West Asia. The age
of the beer will be around 10000B. C. E. Therefore the arise
of beer culture should be from Mesopotamia according to
archeology. People around the world were always fond of
beer. Different kinds of beer were existed during olden days.
Any cereal containing sugar was used to ferment beers sugar
produce alcoholic beverages during fermentation. Beers
usually brewed in domestic scale which accounts to the need
of the people. During the construction of the great pyramid
of Giza, beer were distributed to labor as daily wages in a
total of five to six liters per day. In Europe beer was
produced with sweet fruits which would not rather called as
beer now. In China beer was made with rice and other
cereals rich in starch. Later improvisation in the recipe
introduced barley into beer processing which made a huge
impact. Even beer has a huge part in culture and tradition of
people. It was served to their goddess called Ninkasi during
summers in order to protect their recipe where only few of Traditional way of Brewing:
them were literate and had knowledge about brewing In the Museum’s Egyptian galleries, you can see models
techniques. After the huge response for beer among the excavated from tombs which show wooden figures of
people it was commercialized during industrial revolution. brewers straining mash through a cloth into ceramic vessels.
And MNCs took beer processing a serious profitable sector This visual clue, alongside the research of Delwen Samuel,
and made premium beer which was appreciated by the led us to use a two-stage mash, which we then left to ferment
consumers worldwide. in a vessel containing a harvested yeast culture. The
advantage of a two-stage mash is its simplicity. The cold

@ IJTSRD | Unique Paper ID – IJTSRD33550 | Volume – 4 | Issue – 6 | September-October 2020 Page 1302
 International Journal of Trend in Scientific Research and Development (IJTSRD) @ www.ijtsrd.com eISSN: 2456-6470
mash is made using ambient temperature water and a cultivation started over 3000 BC ago. Presently researchers
malted, ground grain. This mash will contain all the active came up with preliminary evidences to encourage the use of
enzymes required to convert starch to sugar. The second flax seeds by acknowledging its value. Now it has been seen
mash, which is processed at the same time, consists of in all foods from crackers to waffles. Then what, why can’t
ground, unmalted grain. This is mixed with hot water and we introduce such a beneficial seed into brewing. That made
further heated. us intimated to introduce flax seeds into brewing. Because
beer is something that has been loved by most of world. It
There is evidence of heat exposure on ceramic brewing has become a traditional and much needed drink during
vessels found in Egypt. It is unlikely that earthenware would social gatherings. Let’s have a look over the nutritional
be heated above 80 degrees (as it would compromise the benefits of flax seeds.
material), so this was the temperature to which we heated  They are highly rich in Omega-3 essential fatty acids
the hot portion of the mash. Heating grain to this which have some extraordinary favourable benefits for
temperature allows the starches present to unravel, but kills an healthy heart. It has been said that each spoon of this
the enzymes. By preparing the two mixtures separately and power seed contains 1. 8 grams of plant Omega -3s.
then combining them, both the accessible starches and the  It also contains lignans which have both plant estrogen
enzymes required to convert them are present in the final and antioxidant qualities. It contains 75 to 800 times
mix. more lignans than any other plant foods. lignans plays
an significant role in prevention of breast cancer and
The hot mash and the cold mash were mixed together and osteoporosis. It has been identified as dietary tool for
left to cool, so that the enzymes could start to convert the promoting health.
starches in the grains to fermentable sugars. When cool, the  They are rich in both soluble and insoluble dietary fibre.
mash was sieved of any residual grain, directly into the A tablespoon of flax seeds contains 3 grams of fibre
terracotta fermenting vessel, which had been pre-inoculated which accounts upto 12% of daily fibre requirement.
with a harvested yeast strain. More warm water was used to The estimated amount of soluble fibres present in flax
rinse remaining starches and sugars form the grains. The seeds is 20-40% and whereas insoluble is 60 to 80%.
vessel was covered with a muslin cloth and left to ferment. this fibre duo gets fermented by the bacteria in the large
The resulting beer would have been drunk while still actively bowel, bulks up stools and result in more regular bowel
fermenting from the ceramic vessel itself. movements.
 Flax seeds reduces bad LDL cholesterol. Regular
Consumption of flax seeds for 3 months significantly
reduces bad cholesterolupto20%. These effects are
possible due to the fibre in flax seeds usually binds up
with the bile salts and it is excreted by the body.
 Flax seeds have a natural ability to reduce or lower
blood pressure. A Canadian study found that eating 30
grams of flax seeds daily for 6 months reasonably lowers
systolic and diastolic blood pressure by 10 mm Hg and 7
mm Hg respectively. A 2 mmHg reduction in blood
pressure can lower the risk of dying from stroke by 10%
and from heart disease by 7%.
 Flax seeds are great source of plant protein. They are
rich in arginine, aspartic acid and glutamic acid flax seed
protein aids in improving immune system, lowering
cholesterol, in prevention of tumors and had anti fungal
properties with them.
 Due to the presence of insoluble fibre they control blood
sugar level in a significant manner. Research has found
that insoluble fibre slows down the release of sugar into
the blood and reduces blood sugar.
 Flax seeds helps in weight control. They reduce the
feeling of hunger and overall appetite and provides
feeling of fullness. It keeps hunger at Bay.

Nutritional assessment of flax seeds:

You may ask why flax seeds have been chosen by us. But you
will be in wonder if you came to know the nutritional
benefits of flax seeds. It has been called as a power plant
which includes a wholesome of nutrients with it. Flax seeds

@ IJTSRD | Unique Paper ID – IJTSRD33550 | Volume – 4 | Issue – 6 | September-October 2020 Page 1303
 International Journal of Trend in Scientific Research and Development (IJTSRD) @ www.ijtsrd.com eISSN: 2456-6470
Nutrient chart of flax seeds:

Kilning:
 The germinated seed are then killed by slow heating at
80° This process is called kilning.
 The kilning temperature must not harm amylase
enzyme. Furthermore, if kilning temperature is higher,
darker will be the beer produced.

Methodology:
Selection:
Well germitated whole flax seeds are identified and selected.
These whole seeds are sun dried and sauted to remove the
outer shell to get the benefits of both soluble and insoluble
fibers.

Milling
 The dried flax seeds are then crushed between rollers to
produced coarse powder called grist

Malting:
 Flax seeds are first cleaned and then soaked in water for
about 2 days. Then excess water is drained away and the
flax seeds are incubated for 4-5 days to allow
germination
 The germination steps allow the formation of highly
active α-amylase, β-amylase and proteases enzymes as
well as various flavor and color components
 Flax seeds contains considerable amount of protein like
barley. So, if only flax seed are used for beer production,
the final beer will be dark and unstable. Therefore,
protein present in malt should be diluted by adding
additional starch or sugary materials.
 Such sugary or starchy materials are called malt Mashing:
adjuncts and includes dextrose sugar syrup.  Grist is mixed with warm water and the resulting
materials is maintained at 65°C for about 1 hour.
 In doing so, starch is hydrolyzed by amylase enzyme to
produce single sugar, maltose, dextrose etc. similarly,
protein is hydrolyzed by proteolytic enzymes into small
fragments and amino acids.

@ IJTSRD | Unique Paper ID – IJTSRD33550 | Volume – 4 | Issue – 6 | September-October 2020 Page 1304
 International Journal of Trend in Scientific Research and Development (IJTSRD) @ www.ijtsrd.com eISSN: 2456-6470
 The degree of enzymatic hydrolysis is strongly depends
on pH and temperature. β-amylase has optimum activity
at temperature 57-65°C whereas α-amylase has
optimum activity at temperature 70-75°
 The liquid obtained by mashing is called wort. The husks
and other grains residue as well as precipitated proteins
are removed filtration.

Boiling of wort:
 The filtrate is then boiled with stirring for 2-3 hours and
hop flowers are added at various interval during boiling.
 Reasons for boiling of wort:
 For extraction of hop flavor from hop flower Fermentation:
 Boiling coagulate remaining protein and partially  Beer production utilize strain of Saccharomyces
hydrolyze protein and help in removal of protein carlsbergens and S. varum which are bottom yeast and S.
 Boiling inactivates enzymes that were active during cerevisiae which is a top yeast.
mashing, otherwise causes caramelization of sugar  Yeast cells for inoculation are usually recover from
 Boiling also sterilize and concentrate the wort previous fermentation tank by treatment with
phosphoric acid, tartaric acid or ammonium persulphate
to reduce the pH and removed considerable bacterial
contamination.
 Fermentation is usually carried out at 3-4 °C but it may
range from 3- 14° Fermentation usually completes in 14
days.
 During fermentation yeast converts sugar mainly into
ethanol and CO2 plus some amount of glycerol and
acetic acid.
 For fermentation open tank fermenter can be used
however closed fermenter tank is preferred, so that CO2
liberated during fermentation can be collected for later
carbonation step.
 CO2 evolution is maximum by fifth day of fermentation,
there is no evolution of CO2 by 7-9 days because yeast
cells become inactive and flocculate.
 Most beer contains 3. 5-5% alcohol.
Addition of Hops:
 Hops are dried female flower of hop plant
Humuluslupulus. Approximately one quarter pound of
hop flower is added per barrel of beer and up to 2 pound
per barrel of ale.
 Advantages of hop addition in beer are;
 Provide beer with its pungent and aromatic character
 Provide tannin which helps in coagulation of remaining
protein
 Contains α-resin and β-resin which gives bitter flavor as
well as preservative action against gram Positive
bacteria
 Contains pectin which is responsible for foam
characteristic of beer.

@ IJTSRD | Unique Paper ID – IJTSRD33550 | Volume – 4 | Issue – 6 | September-October 2020 Page 1305
 International Journal of Trend in Scientific Research and Development (IJTSRD) @ www.ijtsrd.com eISSN: 2456-6470
Merits of flax beer:
 The fermented flax seed helps in improving gut health. A
study has shown that the fermentation of flaxseed fibers
in the gut changes the micro biota to improve metabolic
health, and could challenge obesity. Obviously we are
using fermented flax seed in brewing which will
simultaneously helps in maintaining healthy gut.
 Flax seeds are generally rich in omega 3 fatty acids
which has heart healthy effects. And excessive
consumption of this beer wouldn’t affect your Health
much than regular ones. omega 3s helps in maintaining
heart’s natural rhythm.
 Flax seeds are effective in reduction of bad LDL
Finishing, Ageing, Maturation and Carbonation: cholesterol and provides good cholesterol.
 The young and green beer is stored in vat at 0°C for  Regular barley beer includes severe health risk such as
several weeks to several months. During this period, flushing, confusion, trouble controlling emotions,
precipitation of protein, yeast, resin and other blackouts, loss of coordination, seizures, drowsiness,
undesirable substances take place and beer become trouble breathing, hypothermia, low blood sugar,
clear. vomiting, diarrhea, bleeding, irregular heartbeat, and
 Ester and other compounds are also produced during others.
ageing which gives taste and aroma.  Such severe health risks are avoided by flax seed beer.
 After ageing, the beer is carbonated by carbon dioxide of Flax seeds contains ‘n’ number of health beneficial
0. 45-0. 52%. nutrients and fibers bestowed with it. Therefore these
 The beer is then cooled, clarified, filtered and packed in side effects can be massively avoided by consuming our
bottles, barrels and cans. flax seed beer.
 Consumption of regular beer includes many
disadvantages such as weight gain, fatigue, irregular
sleep, increasing blood pressure, heart burn, liver
damage etc. Such major drawbacks are heavily
controlled in our flax seed

Conclusion:
Innovations in our daily essentials are much needed part of
life. It improves our way of living. In the modern world
which runs behind money and fame we eventually got no
time to spend or look over our health. Artificial flavoring
beverages and ready to eat foods are taking huge part in our
Overall diagram: daily routine. In order to save time on cooking and to have
some delicious taste we need to sacrifice our health. One of
such drink is beer. It accompanies every family gatherings
and parties. Simultaneously it includes severe health risk.
That’s why we have developed a flax beer which exactly
delivers you a feel of having a delicious beer at the same time
it doesn’t cost your health. Isn’t sounds good??. Now you
don’t need sacrifice your health to have a chill with your
loved ones. flax seeds beer nourishes both your taste buds
and health as well as like a proverb says health is wealth.

Reference:
[1] Gaetano, G.; Costanzo, S.; Di Castelnuovo, A.; Badimon,
L.; Bejko, D.; Alkerwi, A.; Chiva-Blanch, G.; Estruch, R.;
La Vecchia, C.; Panico, S.; et al. Effect of moderate beer
consumption on health and disease: A consensus

@ IJTSRD | Unique Paper ID – IJTSRD33550 | Volume – 4 | Issue – 6 | September-October 2020 Page 1306
 International Journal of Trend in Scientific Research and Development (IJTSRD) @ www.ijtsrd.com eISSN: 2456-6470
document. Nutr. Metab. Cardiovas. 2016, 26, 443–467. [8] Oladokun, O.; Tarrega, A.; James, S.; Smart, K.; Hort, J.;
[Google Scholar] [CrossRef] [PubMed] Cook, D. The impact of hop bitter acid and polyphenol
profiles on the perceived bitterness of beer. Food
[2] Moura-Nunes, N.; Brito, T. C.; Fonseca, N. D.; de
Chem. 2016, 205, 212–220. [Google Scholar]
Aguiar, P. F.; Monteiro, M.; Perrone, D.; Torres, A. G.
[CrossRef] [PubMed][Green Version]
Phenolic compounds of Brazilian beers from different
types and styles and application of chemometrics for [9] Juric, A.; Coric, N.; Odak, A.; Herceg, Z.; Tisma, M.
modeling antioxidant capacity. Food Chem. 2016, 199, Analysis of total polyphenols, bitterness and haze in
105–113. [Google Scholar] [CrossRef] [PubMed] pale and dark lager beers produced under different
mashing and boiling conditions. J. Inst. Brew.
[3] Capece, A.; Romaniello, R.; Pietrafesa, A.; Siesto, G.;
2015, 121, 541–547. [Google Scholar]
Pietrafesa, R.; Zambuto, M.; Romano, P. Use
[CrossRef][Green Version]
of Saccharomyces cerevisiae var. boulardii in co-
fermentations with S. cerevisiae for the production of [10] Piazzon, A.; Forte, M.; Nardini, M. Characterization of
craft beers with potential healthy value-added. Int. J. phenolics content and antioxidant activity of different
Food Microbiol. 2018, 284, 22–30. [Google Scholar] beer types. J. Agric. Food Chem. 2010, 58, 10677–
[CrossRef] 10683. [Google Scholar] [CrossRef]
[4] Budroni, M.; Zara, G.; Ciani, M.; Comitini, F. [11] Zhao, H.; Li, H.; Sun, G.; Yang, B.; Zhao, M. Assessment
Saccharomyces and Non-Saccharomyces Starter of endogenous antioxidative compounds and
Yeasts. In Brewing Technology; Kanauchi, M., Ed.; antioxidant activities of lager beers. J. Sci. Food Agric.
Intechopen: London, UK, 2017; pp. 81–100. [Google 2013, 93, 910–917. [Google Scholar] [CrossRef]
Scholar]
[12] Quifer-Rada, P.; Vallverdú-Queralt, A.; Martínez-
[5] Gómez-Corona, C.; Lelievre-Desmas, M.; Buendía, H. E. Huélamo, M.; Chiva-Blanch, G.; Jáuregui, O.; Estruch,
B.; Chollet, S.; Valentin, D. Craft beer representation R.; Lamuela-Raventós, R. A comprehensive
amongst men in two different cultures. Food Qual. characterisation of beer polyphenols by high-
Preferences 2016, 53, 19–28. [Google Scholar] resolution mass spectrometry (LC-ESI-LTQ-Orbitrap-
[CrossRef] MS). Food Chem. 2015, 169, 336–343. [Google Scholar]
[CrossRef] [PubMed]
[6] Estela-Escalante, W. D.; Rosales-Mendoza, S.;
Moscosa-Santillán, M.; González-Ramírez, J. E. [13] Aquilani, B.; Laureti, T.; Poponi, S.; Secondi, L. Beer
Evaluation of the fermentative potential of Candida choice and consumption determinants when craft
zemplinina yeasts for craft beer fermentation. J. Inst. beers are tasted: An exploratory study of consumer
Brew. 2016, 122, 530–535. [Google Scholar] preferences. Food Qual. Preferences 2015, 41, 214–
[CrossRef] 224. [Google Scholar] [CrossRef]
[7] Giacalone, D.; Ribeiro, L. M.; Frost, M. B. Perception [14] Ferreira, R. H.; Vasconcelos, R. L.; Judice, V. M. M.;
and description of premium beers by panels with Neves, J. T. R. Inovação na fabricação de cervejas
different degrees of product expertise. especiais na região de Belo Horizonte. Perspect. Ciênc.
Beverages 2016, 2, 5. [Google Scholar] [CrossRef] Inf. 2011, 16, 171–191. [Google Scholar]
[CrossRef][Green Version]

@ IJTSRD | Unique Paper ID – IJTSRD33550 | Volume – 4 | Issue – 6 | September-October 2020 Page 1307