The Effect of Magnetic and Electromagnetic Fields On The Morpho-Anatomical Characteristics of Corn (Zea Mays L.) During Biomass Production
The Effect of Magnetic and Electromagnetic Fields On The Morpho-Anatomical Characteristics of Corn (Zea Mays L.) During Biomass Production
The Effect of Magnetic and Electromagnetic Fields On The Morpho-Anatomical Characteristics of Corn (Zea Mays L.) During Biomass Production
A publication of
There are several sources of raw material that have been identified worldwide with some potential to be used
for the production of biofuels; for this reason hard work has been put into increasing the yields per unit area
and increasing the sugar content (as in the case of corn). There are only a small number of studies that have
analyzed the morpho-anatomical behaviour of crops modified for biomass production. For this reason, this
work studied the morpho-anatomical, developmental and physiochemical changes in Zea mays L. plants when
exposed to magnetic and electromagnetic fields with a view to the large-scale production of biomass for the
generation of biofuels. Seeds were exposed to different fields for 21 days, with a control group of seeds that
3
were not exposed. Sowing was carried out in 100 cm trays using coconut fiber as a substrate. The substrate
had been disinfected and moistened prior to use. The sowing trays were placed in a semi-controlled growth
chamber at a light intensity of 3,000 lux, with light cycles of 12 hours and at a temperature of 23°C. The
variables studied were the growth dynamics of coleoptiles and plants, the complete bromatological analysis
and the anatomical structure. The bromatological analysis included the percentage variation of dry matter,
crude protein, ether extract, ash content, organic matter, crude fibre, neutral detergent fibre (NDF), acid
detergent fibre (ADF) and calcium, lignin and water activity. The anatomical structure was studied to identify
relevant histological changes and mainly focused on the arrangement of parenchymatous tissue and the
shape of the cells of which it is comprised. In addition, evaluations were conducted regarding the development
of the crop based on the following indicators: relative growth rate, net assimilation rate, leaf area index, crop
growth rate, absolute growth rate, leaf area duration and specific leaf area. The best results were obtained on
plants whose seeds were treated with electromagnetic fields. A significant increase was seen in the majority of
the indicators studied. This was most evident in the development of the parenchymal tissue and thus the
chlorophyll content, as was clearly seen in the histological sections. It is important to emphasize that the
histological technique allowed us to identify areas of the greatest concentrations of chlorophyll (shown by
differing shades of green) in plants whose seeds had been subjected to electromagnetic fields. An increase in
the chlorophyll level gives us an indirect measure of the high potential these plants offer for producing
biomass.
1. Introduction
In recent years all countries have increased efforts to search for new energy sources that satisfy the demand
of the people. In fact, the biomass production is important for the production of different products. This will
gradually replace the usual energy supply just like that will reduce the environmental, economic and social
impacts (Rosso et al., 2015). Among this diversification of sources, it’s possible to find the biofuel production
based on forest biomass, energy crops, and agricultural wastes among others. This allows to find a
sustainable and lucrative source of energy (Quijano et al., 2012). Within the energy crops that are considered
efficient for the biomass production is corn. Currently, the corn (Zea mays l.) is not only for the production
Please cite this article as: Suarez Rivero D., Ortiz Aguilar J., Marin Mahecha O., Velásquez Perilla P.E., Acevedo P., Santis Navarro A.M., 2016,
The effect of magnetic and electromagnetic fields on the morpho-anatomical characteristics of corn (zea mays l.) during biomass production,
Chemical Engineering Transactions, 50, 415-420 DOI: 10.3303/CET1650070
416
directed to the food chain, but also as a raw material in the bioethanol production. Additionally, the obtained
fresh biomass can be used as for feeding of animals as a source of fiber and energy.
The search for improved energy crops (not just in terms of increased production and quality) does that they
also increase the technological developments (Dvoretsky et al., 2015), whose aims are to obtain biomass
yields in a quick and efficient way, fast vascular growths and with a few agricultural inputs (fertilisers, water,
etc). Based on the above, this research aims to determine the effect on the morpho-anatomical
characteristics, developmental and physiochemical changes in corn (Zea mays L.) when exposed to magnetic
and electromagnetic fields. Histological techniques and bromatological analysis were used to determine the
potential of plants subjected to stimulus, and get a promising amount of biomass energy as a renewable and
environmental friendly raw material.
the EMF. This behaviour had a variation at the end of the study, being the MF those that induced a high LA.
This can be more related to expansion processes of the foliar laminae along with accumulation processes.
3.3 Fresh and Dry Matter
The fresh and dry matter values showed that these were more stimulated when they were applied on a
vegetal material growing with the MF. When determining the ratio between the fresh matter and dry matter, it
was evident that this is more significant for the EMF exposed plants and control plants. In addition, Figure 2
showed that the highest values of dry matter at the end of cycle were occurred in the plants which were
exposed to the EMF
Table 1: Growth Indices used in the physiology of plant and crop, adapted of Santos et al. (2010)
Growth rate Symbol Instantaneous Average value in a time interval Units
value (T2-T1)
Relative Growth Rate RGR 1 ( 2− 1) g/gd
=
( 2 − 1)
Net Assimilation Rate NAR 1 ( 2 − 1) g/cm2d
( 2 − 1)
=
( 2− 1)
( 2 − 1)
Leaf Area Index LAI ( 2 + 1) Dimensionless
= 2 according to the
1
units
Growth rate Symbol Instantaneous
Average value in a time interval Units
value (T2-T1)
Crop Growth Rate CGR 1 1 ( 2 − 1) g/cm2d
= ×
( 2 − 1)
Absolute Growth Rate AGR 2− 1 g/d
=
2− 1
Leaf Area Duration LAD - ( 2 + 1) × ( 2 − 1) cm/d
=
2
Specific Leaf Area SLA 2
+ 1
1 cm2/g
= 2
2
(dw/dt= Derived of the function, LA=leaf area, FA= area of the floor, DL= dry mass foliar, T= time, W= dry
mass).
60
INITIAL FA
50 FINAL FA
40
Leaf Area (cm2)
30
20
10
0
CONTROL MF EMF
Treatment
INITIAL FM
INITIAL DM
4 FINAL FM
FINAL DM
Fresh matter-FM (g) /
Dry matter-DM (g)
0
CONTROL MF EMF
Treatment
.
18
Chlorophyll a
16
Total Chlorrophyll / Carotenoids (µg/ml)
Chlorophyll b
Total Chlorophyll
Chlorophyll a / Chlorophyll b /
14 Carotenoids
12
10
0
CONTROL MF EMF
Treatment
should be noted that the NAR was the variable with the highest difference with regard to the control and the
magnetic field. The maximum values of NAR were translated in a higher photosynthetic efficiency and
therefore a high biomass accumulation
70
60 Crude fibre
NDF
ADF
Crude fibre/ NDF / ADF (%)
50
40
30
20
10
0
CONTROL MF EMF
Treatment
Figure 4: Crude fibre content (CF), neutral detergent fibre (NDF), and acid detergent fibre (FDA)
When making the analysis of the aqueous activity (Aw) in the figure 5 it is certain that the values are similar for
different treatments and the control (0.95 for the CEM, 0.93 in CM and 0.92 for plant them Control). The rest of
variables showed in the same figure also show similar behaviours, standing out the content of protein that
tends to be high in the plants treated with the CEM and that can increase the nutritional value of the produced
biomass.
420
8
Nitrogen
Crude Protein
Ether Extract
Ether Extract / Water Activity (%)
Water Activity
6
Nitrogen / Crude Protein /
0
CONTROL MF EMF
Treatment
4. Conclusions
During the experiment has been found that plants exposed to EMF produced more biomass quantity together
with high quality. All this was based on the bromatological analysis, especially for by the low obtained contents
of NDF and ADF. The NDF and ADF contain lignin and this is associated with the quality of the biomass used
mainly for animal feeding.
High contents of chlorophylls and carotenoids were obtained in plants exposed to the EMF. Chlorophylls are
responsible for the synthesis processes. And this, it seems to be related to the increased production of
biomass and protein synthesis obtained in the bromatological analysis.
From the performed analysis it is possible to observe the benefits (especially in the biomass production) that a
large scale application of the EMF during the growth and development of corn offers.
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