Figure 2: UV-Vis Spectra Recorded As A Function of Reaction Time of Silver Nanoparticles

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Initially, the stock cultures of bacteria were revived by inoculating broth media and grown at 37ºC for 18

hrs. The agar plates were poured by muller hinton media and wells were made in the plate. Each plate was
inoculated with 18 h old cultures (100 μl, 10-4cfu) and spread evenly on the plate. After 20 min, the wells
were filled with desired quantity (20, 40, 60, 80 and 100 μl) of nanoparticles solution. All the plates were
incubated at 37ºC for 24 h and the diameter of inhibition zone was noted.

I. Results and discussion


When leaf extract of P.glabrum was added to aqueous 1mM silver nitrate solution which leads to
the appearance of dark brown colour within 10 min. The colour change was arising due to the property of
quantum confinement which is a size dependent property of nanoparticles which affects the optical property
of the nanoparticles 30. UV-Visible absorption spectroscopy is widely used to study the optical properties and
electronic structure of nanoparticles. The process of bio reduction of silver ions was gradually monitored in
UV- Vis spectroscopy Figure 2 depicts a series of absorption spectra recorded from the solution of silver
nanoparticles at different time intervals. The absorption spectra showed an intense peak at 429 nm due to the
surface Plasmon resonance (SPR) band of silver nanoparticles. Noble metals exhibit unique optical
properties due to the property of surface plasmon resonance (SPR) which is the collective oscillation of the
conduction electrons induced by the interacting electromagnetic field. The size and shape of metal
nanoparticles determine the spectral position of plasmon band absorption as well as its width 31 .The
appearance of single prominent peak shows that the particles are spherical and uniform in size 32 which is
further confirmed by SEM photograph.

2.2

48hrs
2.0

1.8

1.6 12hrs 6hrs 4hrs


24hrs
2hrs
Absorbance

1.4

1.2

1.0

0.8

0.6

0.4

0.2

400 500 600 700 800

wavelength (nm)

Figure 2: UV-Vis spectra recorded as a function of reaction time of silver nanoparticles

1.2

250nm

0.8
absorbance

0.4

0.0

-0.4
200 250 300
/nm

Figure 3: UV-Vis spectrum recorded at lower wavelength of silver nanoparticles

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