GENERAL  ARTICLE

Biosensors
S Chauhan, Vibhuti Rai and H B Singh

Biosensors function by coupling a biological sensing ele-

ment with a detector system using a transducer. They are
widely used in diagnostics, pharmaceutical research, fer-
mentation-based industries and environmental and pollu-
tion monitoring.

Introduction
Biosensors consist of a biological entity that can be an enzyme,
antibody, or nucleic acid that interacts with an analyte and (left) Swarna Baish is
produces a signal that is measured electronically. Each biosen- currently working as
sor, therefore, has a biological component that acts as the sensor Senior Research Fellow in
Plant Pathology Labora-
and an electronic component to transduce and detect the signal.
tory, National Botanical
A variety of substances including nucleic acids, proteins (par- Research Institute,
ticularly antibodies and enzymes), lectins (plant proteins that Lucknow. She is working
bind sugar moieties) and complex materials (organelles, tissue on isolation and character-
ization of thermotolerant
slices, microorganism), can be used as the biological compo-
enzymes from thermo-
nents. In each case it is the specificity of the biological compo- philic fungi.
nents for an analyte (or group of related analytes) that makes the
biomolecules attractive as sensing component. For example, a (right) Vibhuti Rai is
single strand of DNA can be used as a biomolecular sensor that Head, School of Life
Sciences, Pt. Ravi Shankar
will hybridize only to its complementary strand under appropri- University, Raipur. He
ate conditions. The signal, which can be electrical, optical or specializes in the field of
enzymes from thermo-
thermal, is converted by means of a suitable transducer into a
philic fungi.
measurable electrical parameter such as current or voltage (Fig-
ures 1a, 1b). Biosensor probes are attaining increasing sophisti- (center) B Singh is Head
and Scientist, Plant
cation because of the fusion of two technologies: microelectron-
Pathology Division,
ics and biotechnology. Biosensors provide a useful means for National Botanical
measuring a wide spectrum of analytes (e.g., gases, ions and Research Institute,
organic compounds, or even bacteria) and are suitable for stud- Lucknow. He specializes
in the field of biocontrol of
ies of complex microbial environments.
plant diseases.

In 1956, Leland C Clark Jr., who is known as the father of

Biosensors, published his definitive paper on the oxygen

RESONANCE  December 2004 33

 GENERAL  ARTICLE

Figure1. electrode in which he described the fabrication of electrochemi-

(a) (top): Construction and
cal sensors. He carried this out by using an enzyme transducer.
mode of operation of a bio-
Clark’s oxygen electrode was the enzyme glucose oxidase, en-
sensor.
(b) (bottom): Schematic out- trapped in dialysis membrane. The method of detection is based
line of biosensor. on the decrease in oxygen concentration that is proportional to
glucose concentration.

Guilbault and Montalvo were the first to provide a detailed

description of potentiometric enzyme electrode, i.e. the urea
sensor, based on urease, immobilized at an ammonium–selec-
tive liquid membrane electrode. The use of thermal transduc-
ers for biosensors was proposed in 1974 and the new devices
were christened thermal enzyme probes. In 1975, Divis sug-
Keywords
gested that bacteria could be harnessed as the biological ele-
Electrochemistry, optical/ piezo
electric devices, microbial sen-
ment in microbial electrodes for the measurement of alcohol
sor. content. Lubbers and Opitz coined the term ‘optode’ to

34 RESONANCE  December 2004

 GENERAL  ARTICLE

Enzyme Gel (5µm)

Tip of fibre optic
enzyme sensor Carbon black (5µm)

Silicone indicator
140 µm (25µm)

Polyester support
(110µm)

describe a fiber-optic sensor with an immobilized indicator to Figure 2. Enzyme optrodes

measure carbon dioxide or oxygen (Figure 2). schematic structure.

Biosensors can be broadly classified as follows, based on the

principle involved.

A. Electrochemical Sensors

In this configuration, sensing molecules are either coated onto

or covalently bonded to a probe surface. A membrane holds the
sensing molecules in place, excluding interfering species from
the analyte solution. The sensing molecules react specifically
with compounds to be detected, sparking an electrical signal
proportional to the concentration of the analyte. The bio-mol-
ecules may also respond to an entire class of compounds such as
opiates and their metabolites. The most common detection
method for electrochemical biosensors involves measurement of
current, voltage, conductance, capacitance and impedance.

B. Optical Sensors

In optical biosensors, the optical fibers allow detection of analytes

on the basis of absorption, fluorescence or light scattering. Since
they are non-electrical, optical biosensors have the advantages of
lending themselves to in vivo applications and allowing multiple
analytes to be detected by using different monitoring wave-
lengths. The versatility of fiber optics probes is due to their
capacity to transmit signals that reports on changes in wave-
length, wave propagation, time, intensity, distribution of the
spectrum, or polarity of the light. In general, acquisition of the

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 GENERAL  ARTICLE

Luciferase Optical
signal from these devices is accomplished through flexible cables,
Electrode which can transmit light to the biological component.

Optrodes use fiber optics for performing optical measurement

A luciferase systems light
emitting reaction proceeds away from the measuring locations (e.g., intra–arterial determi-
via the reaction of mo- nation using FIA systems). A powerful and sensitive analytical
lecular oxygen with re- methodology has been constructed based on the luciferin/lu-
duce flavin (FMNH) and ciferase bioluminescence reaction.
aliphatic aldehyde as fol-
lows: C. Piezoelectric Sensors

FMNH2 + RCHO + O 2 → In this mode, sensing molecules are attached to a piezoelectric

FMN + RCOOH + H 2O + surface – a mass to frequency transducer – in which interactions
hν between the analyte and the sensing molecules set up mechani-
The system may be regen- cal vibrations that can be translated into an electrical signal
erated by supplying FMN proportional to the amount of the analyte (Figure 3). Example of
reductase together with such a sensor is quartz crystal micro or nano balance.
NAD(P)H.
D. Field Effect Transistor (FET)

This method makes use of an ion-sensitive field effect transistor

Figure 3. Piezo sensor
based on molecular recog-
(ISFET) built on standard technology that produces source,
nition by specific molecule drain and gate regions. The gate uses an ion sensitive membrane
attachment which leads to that renders ISFET capable of biochemical recognition in the
mechanical vibrations. presence of the analyte with the increase in local ion concentra-

B C
ANTIGENS

A A A (specific Molecule)
Frequency transducer
(converts biological B B (Non specific)
signals to mechanical
vibrations) C (Non specific)
A A

C Molecule
specific site
B
A
C C
B

36 RESONANCE  December 2004

 GENERAL  ARTICLE

Immobilized enzyme
membrane
10µm Inactivated enzyme
membrane

Si3N4
n+ P n+ P SiO2

350µm
Sapphire

Au

tion. Microelectrodes are created on a silicon nitride surface Figure 4. Ion sensitive field
using vapour deposition method and partially insulated by tita- effect transistor.
nium oxide (Figure 4). The hardware component consists of an
electrode system that could either be a conventional platinum or
silver–silver chloride microelectrode and a field effect transistor
with an ion sensitive gate or gas sensing electrode.

Biosensing Method

The essence of the biosensor is matching the appropriate bio-

logical and electronic components to produce a relevant signal
during analysis. Isolation of the biological component is neces-
sary to ensure that only the molecule of interest is bound or
immobilized on the electronic component or the transducer.
The stability of the biological component is critical, since it is
being used outside its normal biological environment.

Attachment of the biological component to the electronic com-

ponent is vital for the success of these devices. If the biological
component is destroyed in the process of binding or if it binds
with the active site unavailable to the analyte, the biosensor will
not function. Attachment can be accomplished in a variety of
ways, such as covalent binding of the molecule to the detector

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 GENERAL  ARTICLE

(usually through a molecular cross-bridge), adsorption onto the

surface entrapment in porous material, or micro encapsulation.
Ultra-thin applications of biological material are usually depos-
ited on transducers by using the Langmuir – Blodgett or mo-
lecular self-assembly technique.

Types of Biosensors

BOD Sensor: A biosensor consisting of immobilised yeast,

Trichosporon cutaneum and an oxygen probe was developed for
BOD estimation. The BOD biosensor includes an oxygen elec-
trode that consists of a platinum cathode and an alluminium
anode bathing in saturated KCl solution and a Teflon mem-
brane. Yeast cells are immobilized on a porous membrane and
are trapped between the pores and the Teflon membranes. Oxy-
gen consumption by the immobilized microorganisms causes a
decrease in current until a steady state is reached. The BOD
biosensor measures BOD at 3-60 mg/L.

Methane Biosensor: This biosensor consists of immobilized

methanotrophic bacteria (Methylomonas flagellata) in contact
with an oxygen electrode. The immobilized bacteria use meth-
ane as well as oxygen according to the following reaction.

CH4 + NADH2 + O2 → CH3OH + NAD + H2O,

where NAD is nicotinamide adenine dinucleotide and NADH2

is the reduced form of the coenzyme. Oxygen consumption leads
to a decrease in current, which is proportional to the methane
concentration in the sample.

Ammonia and Nitrate Biosensors: Ammonia biosensor, based

on amperometry, consists of immobilized nitrifying bacteria
(e.g., Nitrosomonas europaea) and a modified oxygen electrode.
This biosensor, with a lifetime of approximately 2 weeks was
used for ammonia determination in waste waters based on the
conversion of nitrate to N2O by an immobilized denitrifying
bacterium Agrobacterium sp. The nitrate biosensor has been used
to measure nitrate profiles in biofilms in environment samples.

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Microbial Biosensor: A microbial sensor consists of a microor-

ganism immobilized on a membrane and an electrode. The
principle of a microorganism sensor is based on either the
change in respiration or the amount of metabolites produced as
a result of the assimilation of substrates by the microorganism.
Recently, microbial sensors using thermophilic bacteria have
been developed (Table 1). They reduce contamination of other Table 1. Examples of mi-
microorganisms by the use of high temperature. For example crobial biosensors.

Substances measured Microorganism employed Detected Useful concentration

(Class and Example) substance* measured

Alcohol Trichosporon brassicae O2 Below 22mg litre–1

Ethanol (or Acetobacter xylinium)

Amino acid
L-arginine Streptococcus faecalis NH 3 10 –5 ×5×10 –5 mol/litre
L-glutamate Escherichia coli CO2 10 –3 –10 –5 mol/litre

Antibiotics
Nystatin Yeast cells O2 0.5 to 80 units/ml
Cephalosporin Citrobacter freundii H+ Below 22 mg/litre

Co-factors
NAD+ Escherichia coli/NADase NH 3 8×10–4 to 5×10–5 mol/litre

Gases
Methane Methylomonas flagelata O2 Upto 6.6 × 10 –3 mol/litre

Organic acids
Formate Clostridium butyricum Fuel cell Upto 1.0 g/litre

Salts
Nitrate and nitrite Azotobacteria vinelandii NH 3 8×10 –4 to 10 –5 mol/litre

Sugars
General Bacteria from human H+ 10 –4 to 10 –5 mol/litre
dental plaque

Vitamins
Nicotinic acid Lactobacillus arabinosus H+ 5×10 –8 to 5×10 –6 gm/ml

* Usually detected by potentiometric or amperometric methods

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 GENERAL  ARTICLE

BOD and carbon dioxide sensors are constructed by using

thermophilic bacteria isolated from a hot spring.

Microbiosensors have many advantages:

1. They can be implanted in the human body and are suitable

for in vivo detection.

2. They can be integrated on one chip and are useful for measur-
ing various substrates in a small amount of sample solution
simultaneously.

3. Semiconductor fabrication technology can be applied to

microbiosensors. It is possible to develop disposable trans-
ducers for biosensors through mass production.

Microbiosensors are based on ion sensitive field effect transistor

(ISFET) and were first reported by Bergveld (1970). Matsuo et
al. (1974) improved the ISFET using silicon nitride as the gate
insulator to construct micro pH sensitive devices. They show
rapid response, low power consumption and low noise.

Urea Sensor: A urea sensor consists of urease immobilized on

membrane and a pH electrode. Urease-catalyzed reaction cause
pH changes so that ISFET can be used as a transducer. The urea
sensor gives the linear relationship between the initial rate of the
output gate voltage and the logarithm value of urea concentra-
tion in the range 16.7 to 167 mM and can be used for 20 days with
slight degradation of the enzyme activity.

Alcohol Sensor: This system consists of membrane bound alco-

hol dehydrogenase (ADH), aldehyde dehydrogenase (ALDH)
and an electron transfer system which can be used in conjunc-
tion with an ISFET.

Hypoxanthine and Inosine Sensor: Hypoxanthine is measured

on the basis of the reaction catalyzed by xanthine oxidase (XO).
The pH change caused by uric acid is detected by using a Si
ISFET. The oxidation of hypoxanthine to uric acid by xanthine
oxidase is initiated immediately after injection. The response to
inosine, however, has a time lag of 90 sec after injection. This

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Hypoxanthine and Inosine Sensor

To maintain quality, evaluation of freshness is important in the fish industry. When a fish dies, adenosine
5’ triphosphate (ATP) decomposition in the fish meat occurs and adenosine 5’ diphosphate (ADP) and
adenosine 5’ monophosphate (AMP) and related compounds are generated where IMP, HxR, Hx, X and
U stands for inosine 5’ monophosphate, inosine, hypoxanthine, xanthine and uric acid respectively.There
comes the use of hypoxanthine and inosine sensor.

ATP→ ADP→AMP→ IMP→HxR→Hx→X→U,

where IMP, HxR, Hx, X and U stands for inosine 5’ monophosphate, inosine, hypoxanthine, xanthine and
Uric acid, respectively, consequently, Hx accumulation with an increase in storage time can be used as an
indicator of fish meat freshness. Therefore, simple and rapid methods for the determination of Hx and
HxR are required in the seafood industry.

phenomenon is attributed to the three-step reaction. On the

basis of this time lag the sensor can determine inosine and hypo-
xanthine simultaneously.

Glucose and Carbon Dioxide Sensors using Micro-

oxygen Electrode

The Glucose Sensor

The glucose sensor is fabricated by immobilizing glucose oxi-

dase (GOD) on the membrane of the oxygen electrode. The
glucose sensor responds as soon as the glucose solution is in-
jected into the buffer solution and reaches a steady state in 5 to
10 min. The sensor responds almost linearly for glucose concen-
tration between 0.2 and 2mM, which is comparable to conven-
tional glucose sensors.

A microbial CO2 sensor using this oxygen electrode was con-

structed by Suzuki et al. The autotrophic bacterium S17, which
can grow with only carbonate as the carbon source was used.
Bacterial cells are immobilized on a micro oxygen electrode.
The sensitive area of the oxygen electrode is immersed in 0.2%
sodium alginate solution containing S17 whole cells, then
removed and immediately immersed in 5% CaCl2 solution to
form bacteria immobilized calcium alginate gel. The response

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 GENERAL  ARTICLE

time is 2 to 3 min. Carbon dioxide was supplied by acidification

of NaHCO3, the concentration of which can be related to CO2
concentration. Linear relationship is obtained between the cur-
rent decrease and NaHCO3 concentration in the range 0.5 to
3.5mM. The lowest detection limit is 0.5mM NaHCO3 within
the margin of the noise amplitude (Figure 5).

Integrated Multibiosensor

These biosensors are based on the ISFETs and electrodes, coated

with enzyme immobilized membrane. An enzyme-immobilized
membrane should be precisely deposited onto a gate region or
small working electrode. Different enzyme membranes can be
prepared without mixing.

Applications

Figure 5. Structure of mi- Biosensors have many uses in clinical analysis, general health
cro oxygen electrode show- care monitoring, veterinary and agricultural applications,
ing different parts. industrial processing and monitoring, and environmental pollu-

2 Gas permeable membrane

a Agarose gel +0.1 M kcl a'

a

SiO 2 layer

b b'
15 cm

Gold b b'
Electrode Gold Electrode

c c'

c Silicon indicator c'

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 GENERAL  ARTICLE

Nucleic Acid Biosensor

It depends on the ability of a single stranded nucleic acid to hybridize with another fragment of DNA by
complementary base pairing. Technological innovation is introduced in the manner in which the nucleic
acid oligomer is attached to the surface of the detector and the manner in which the hybridized nucleic acid
is detected and transduced into a measurable signal. Ammonia derivatised oligonucleotides can be
detected can be attached to glass (SiO2) surfaces such as fiber-optic cables, glass beads or microscopic
slides through covalent bonding with a chemical linker.

A nucleic acid biosensor that utilizes evanescent wave technology by using short fragments of nucleic
acids that are small enough to reside within the field of the evanescent wave. They were able to detect
fluorescent – labeled DNA hybridizing to their complementary immobilized probes in a flow cell.
Fluorescence was monitored and reported as a change in the output voltage. Nucleic acid biosensors are
potentially useful in the field of rapid DNA sequencing as well as in clinical applications.

The Biosensor described by Eggers et al. integrates microelectronics, molecular biology and computa-
tional sciences in an optical electrode format. Their device can detect hybridization and report on the
spatial address of the hybridization signal on a glass surface or a silicon wafer, to which the DNA probes
are attached. Several different DNA oligomers can be attached to the optical electrode at different
locations. The DNA on the biosensor is then hybridized to DNA that is free in solution. The free DNA must
be labeled, usually with a fluorescent, luminescent, and radioisotope decay 32 P signal. The signal is
detected by charge coupled device (CCD), which is extremely sensitive. The computer identifies the
location of the affected pixels and forms the signals into a recognizable array not only in this technology
suitable for rapid DNA sequencing, but it is also applicable to the rapid detection of many different gene
sequences from DNA extracted from a consortium.

tion control. The advantages are likely to include low cost, small
size, quick and easy use, as well as a sensitivity and selectivity
greater than the current instruments.

The advent of cheap, user-friendly biosensors will revolutionize

the practice of healthcare monitoring and enables more in-depth
diagnosis on a metabolic basis. The introduction of suitable
biosensors would have considerable impact in the following
areas:

Clinical and Diagnostic Applications: Bench top biosensors of

the electrochemical variety are used now in clinical biochemis-
try laboratories for measuring glucose and lactic acid. A key
feature of this is the ability for direct measurement on undiluted
blood samples. Consumer self-testing, especially self-monitor-

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 GENERAL  ARTICLE

Suggested Reading ing of blood components is another important area of clinical

medicine and healthcare to be impacted by commercial
[1] A F Collings and F Caruso,
Biosensors: recent advances,
biosensors. Current methods are based on colorimetric dry
Rep. Prog. Phys. Vol.60, reagent chemistry often in conjunction with a portable reflec-
pp.1397-1445,1997. tance meter.
[2] A P F Turner, I Karube and
G S Wilson (Eds.), Biosen- Biosensors offer the potential of reusable systems and other
sors: Fundamentals and Ap-
advantages by employing electrochemical detection rather than
plications, Oxford University
Press, New York.
colour changes to help alleviate the problems of those with poor
[3] W Gopel, T A Jones, M eyesight (some of them diabetics who are often heavy users of
Kleitz, J Lundstrom and T biosensors for glucose determination). Reusable sensors also
Seiyama (Eds.) , Sensors: A
permit calibration and quality control unlike the present dispos-
comprehensive survey; Chem-
ical and Biochemical sensors
able sticks where only one measurement can be carried out. Such
(Weinheim:VCH), Vols. 2 testing will improve the efficiency of patient care, replacing the
and 3. 1991. often slow and labour intensive present tests. It will bring
[4] R S Sethi. Transducer as-
clinical medicine closer to bedside, facilitating rapid clinical
pects of biosensor, Biosens.
Bioelectronics, Vol.9, pp. 243-
decision-making. Examples of potential biosensor forms and
264, 1994. their uses in diagnostic medicine are:
[5] J Wang, Analytical Electro-
chemistry, Second Edition, Industrial Applications: Along with conventional industrial
John Wiley & Sons, 2000. fermentation producing materials, many new products are being
produced by large-scale bacterial and eukaryotes cell culture.
The monitoring of these delicate and expensive processes is
essential for minimizing the costs of production; specific
biosensors can be designed to measure the generation of a
fermentation product.

Environmental Monitoring: Environmental water moni-

Address for Correspondence toring is an area in which whole cell biosensors may have
S Chauhan, Vibhuti Rai and
substantial advantages for combating the increasing num-
H B Singh
School of Life Sciences ber of pollutants finding their way into the groundwater
Pt. Ravishankar Shukla systems and hence into drinking water. Important targets
University for pollution biosensors now include anionic pollutants
Raipur 492 010, India.
such as nitrates and phosphates.
*
National Botanical Research
Institute
The area of biosensor development is of great importance to
Rana Pratap Marg
Lucknow226 001, India. military and defense applications such as detection of chemical
Email: [email protected] and biological species used in weapons.

44 RESONANCE  December 2004