Brilian Pamungkas (6008201006)

example 2.3
Cs r Cs/r 1/r 1/Cs r/Cs
1 0.2 5 5 1 0.2
2 0.22 9.090909090909 4.5454545455 0.5 0.11
3 0.3 10 3.3333333333 0.3333333333333 0.1
5 0.45 11.11111111111 2.2222222222 0.2 0.09
7 0.41 17.07317073171 2.4390243902 0.1428571428571 0.058571
10 0.5 20 2 0.1 0.05
15 0.4 37.5 2.5 0.0666666666667 0.026667
20 0.33 60.60606060606 3.0303030303 0.05 0.0165

Langmuir Plot

Langmuir-Plot (All Data) La

70 25
60

50 f(x) = 2.71753349145469 x − 0.102919802732182 20

f(x) = 1.5866048
40
Cs/r

15
30

20 10
10

0 5
0 5 10 15 20 25
Cs 0
0 2

Cs/r = KM/rmax + Cs/rmax

slope = 1/rmax
intercept = KM/rmax
rmax = 1/slope
KM= intercept/slope

slope 2.7175
intercept -0.1029
All Data
rmax 0.367985280588776
Km -0.037865685372585

Lineweaver-Burk Plot

Lineweaver-Burk Plot (All Data) Lineweave

6
f(x) = 3.03867281573321 x + 2.224903446203 6
5
5 f(x) = 3.4575111518788
4
 Lineweaver-Burk Plot (All Data) Lineweave
6
f(x) = 3.03867281573321 x + 2.224903446203 6
5
5 f(x) = 3.4575111518788
4
4
3
1/r

3
2

1 2

0 1
0 0.2 0.4 0.6 0.8 1 1.2
1/Cs 0
0 0.2 0.4

1/r = 1/rmax + (KM/rmax*1/Cs)

slope = KM/rmax
intercept = 1/rmax
rmax= 1/intercept
KM = slope/intercept

slope 3.0387
intercept 2.2249
All Data
rmax 0.449458402624837
Km 0.20201285569007

The Eadie-Hostfee Plot

The Eadie-Hostfee plot (All Data) The Ead

0.6
0.6
0.5
0.5
0.4 f(x) = − 1.21143558034183 x + 0.449942339704455
0.4 f(x) = − 1.8923207707
0.3
r

0.2 0.3

0.1 0.2

0 0.1
0 0.05 0.1 0.15 0.2 0.25
r/Cs 0
0.04 0.06 0.08

r = rmax - (KM*(r/Cs)) r = rmax/(1+KM/Cs)

slope = -KM
intercept= rmax

All Data Cs <= 10

 rmax= 0.4499 rmax= 0.5386
KM= 1.2114 KM= 1.8923

nonlinear regresion
rnon linear = rmax*Cs/(KM+Cs)
eror = [(r-rnonlinear)^2]
Cs r rnonlinear (all data) eror (all data) rnonlinear eror
1 0.2 0.200324352841265 1.05204766E-07 0.1604984837 0.0015603697871
2 0.22 0.279087203708977 0.003491297642 0.256186524 0.0013094645181
3 0.3 0.321180796447198 0.000448626138 0.3197259629 0.000389113612
5 0.45 0.365252432676777 0.007182150167 0.3988677845 0.002614503462
7 0.41 0.38807410599517 0.000480744828 0.4462029366 0.00131065262
10 0.5 0.407153925268407 0.008620393593 0.4897975343 0.0001040903062
15 0.4 0.423342437107733 0.00054486937
20 0.33 0.431929228233973 0.010389567568
SSE 0.031157754512 0.0072881943053

misal
rmax = 1
KM= 1

Hasil Dari Solver

All Data Cs <= 10
rmax= 0.459915 rmax= 0.6344278243
KM= 1.295852 KM= 2.9528586783

Data Yng didapat

Cs r r langmuir r lineweaver r eadie r nonlinear
1 0.2 5 0.189984041341 0.203445781 0.2003243528413
2 0.22 4.54545454545455 0.267076183481 0.2801893255 0.279087203709
3 0.3 3.33333333333333 0.308851689419 0.3204872489 0.3211807964472
5 0.45 2.22222222222222 0.353027564392 0.3621566797 0.3652524326768
7 0.41 2.4390243902439 0.376081233546 0.3835277785 0.3880741059952
10 0.5 2 0.395449170941 0.4012879747 0.4071539252684
15 0.4 2.5 0.411949840987 0.4162811355 0.4233424371077
20 0.33 3.03030303030303 0.420727564177 0.4242058516 0.431929228234

Reaction Rate vs Substrate concentration curves Predicted from Michaelis-Menten Equation with
Values Obtained by Four Different Method
0.6

0.5

0.4
Data
linew
 0.6

0.5

0.4
Data
linew
langm
0.3
Eadie
r

Non

0.2

0.1

0
0 5 10 15 20 25

Cs

Untuk Cs <= 10 mmol/L Semua Data Beda parameter

Metode
rmax KM rmax KM rmax KM
0.630279 2.9255640993319 0.367985280589 -0.037865685
Langmuir -41.61545538178 -101.2943
Lineweaver- 0.514139 1.77763496143959 0.449458402625 0.2020128557
Burk -12.58034068947 -88.63586
0.5386 1.8923 0.4499 1.2114
Eadie-Hostfee -16.46862235425 -35.98267
Nonlinear 0.634428 2.95285867829043 0.45991504351 1.2958518871
Regresion -27.50711335446 -56.11534

Kesimpulan
Dari perhitungan data dengan cara linear diketahui bahwa Lineweaver dan Edie memberikan data yang lebih ba
perbedaan parameter yang tidak begitu jauh antara perhitungan Cs <= 10 maupun dengan semua data Hal ini d
menitik beratkan pada bagain bawah data (Cs < 10) dan pada CS > 10 tidak begitu berpengaruh. Sedangkan pad
parameter yang cukup signifikan bahkan nilai KM nya negatif pada perhitungan semua data yang menunjukan b
tidak dapat diterapkan dengan Langmuir. Sedangkan Cara nonlinear regresion cenderung bagus digunakan di ke
 1/r lineweaver r lineweaver r eadie 1/r langmuir r langmuir
5.2636 0.18998404134053 0.20344578095324 5.435 0.18399264029
3.74425 0.26707618348134 0.28018932552781 4.07625 0.24532352039
3.2378 0.30885168941874 0.32048724889586 3.623333333333 0.27598896044
2.83264 0.35302756439223 0.36215667965354 3.261 0.30665440049
2.659 0.37608123354645 0.38352777845434 3.105714285714 0.32198712052
2.52877 0.39544917094081 0.40128797474 2.98925 0.33453207326
2.42748 0.41194984098736 0.41628113549724 2.898666666667 0.34498620055
2.376835 0.42072756417673 0.42420585157038 2.853375 0.35046217199

Langmuir-Plot (Cs <= 10)

25

20
f(x) = 1.58660484596389 x + 4.64170920778976

15

10

0
0 2 4 6 8 10 12

Slope 1.5866
intercept 4.6417
Cs <= 10
rmax 0.63027858313375
KM 2.9255640993319

Lineweaver-Burk Plot (Cs <= 10)

f(x) = 3.45751115187882 x + 1.94501342267085

 Lineweaver-Burk Plot (Cs <= 10)

f(x) = 3.45751115187882 x + 1.94501342267085

0.2 0.4 0.6 0.8 1 1.2

slope = 3.4575
Intercept = 1.945
Cs <= 10
rmax 0.51413881748072
KM 1.77763496143959

The Eadie-Hostfee plot (Cs <= 10)

0.6

0.5

0.4 f(x) = − 1.89232077075659 x + 0.538602059129121

0.3

0.2

0.1

0
0.04 0.06 0.08 0.1 0.12 0.14 0.16 0.18 0.2 0.22
enten Equation with Parameter

Data awal
lineweaver
 Data awal
lineweaver
langmuir
Eadie
Non linear regresion

25

Metode terbaik

mberikan data yang lebih bagus ditunjukan dengan

engan semua data Hal ini disebabkan kedua metode
rpengaruh. Sedangkan pada Langmuir ada perbedaan
ua data yang menunjukan bahwa Metode Michaelis-Menten
rung bagus digunakan di kedua keadaan Cs <= 10 dan Cs>10
(empty)